ECT2 cDNA ORF clone, Mesitornis unicolor(brown roatelo)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECT2
Entrez Gene ID	104546612
Full Name	epithelial cell transforming 2
Gene Type	protein-coding
Organism	Mesitornis unicolor(brown roatelo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010192047.1	XP_010190349.1	protein ECT2 isoform X1
XM_010192048.1	XP_010190350.1	protein ECT2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ECT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe84070	XM_010192047.1 Latest version!	Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMe84071	XM_010192048.1 Latest version!	Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECT2 ( XM_010192047.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010190349.1 Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe84070
Clone ID Related Accession (Same CDS sequence)	XM_010192047.1
Accession Version	XM_010192047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	protein ECT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010207202.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACAGTTTGGT GGTGTCTGAA ACCGGGAGAA GCCTCTTGGC TGATTCTTCT 
GTTCTTGATT CAAAAATTAT AGAAACCTCC AAAGAGAATG CATTGCCTGT ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA GGAAGCAGGG 
AAACATGAGG AGCTTCTGAA AGCCTTGGAG GAAATTAAAG TGCCCTACAT AAAGACAGAC 
ACGATAGAAG AGCTTGGAGA TTCTGATTCC CCAGAATTTG AGACCATCTT TGTTGTGTCA 
GACTTCCAAG ACTCTATCTT CAACAACCTC TGCAAAGCAG ATTGCCGTGT CATTGGACCT 
CCAGTAGTAC TGCACTGTGC ACAAAAAGGA GAGCCTTTAC CTTTCTCCTG TCGTCCACTG 
TACTGTGCAT GTATGTTGAA CTTGGTGCTG TGCTTTACGG GATTCAGAAA GAAGGATGAA 
TTAGTTAATC TCGTGACCTT AGTTCATCAT ATGGGTGGAA TTATTCGAAA GGACTTCAGC 
TCGAAAGTTA CACATCTGGT GGCTAACTCA ACACATGGAG ACAAATTCAG AATTGCTGTA 
AGCCTTGGTA CTCCTATCGT GAAAGCTGAG TGGATTTATA AAGCTTGGGA GAAAAGGAAT 
GAAATTAATT TCTGTGCAGC TGAAGATTAC TTTAGAAATC AGTTCAAGGT TCTTCCCTTC 
CAGGATTGCA TGTTAAGTTT CCTTGGATTC TCTGATGATG AGAAAGCTAA TATGGAAGAA 
ATGACAGAAA TGCAAGGAGG ATGTTATTTA CCAGTTGGTG ATGAAAGGTG TACACACTTA 
GTGGTTGAAG AGAGTACAGT AAAAGATCTT CCATTTGAAC CTTTAAAAAA ACTTTATGTT 
GTAAAGCAAG AGTGGTTCTG GGGAAGCATA CAAATGGATG CCAGAGCTGG AGAGTCCATG 
TATTTATTTG AGAAGTCAGA AAGTCCTGGC CTCAAGAAGT CTGTGTCACT ACTTTCTCTG 
AATACTCCAA ACAGCAATCG CAAAAGACGT CGTTTGAAAG AGACCCTTGC ACAACTGACC 
AGAGAAACAG ATATGTCACC ATTCCCTCCT CGGAAACGCC CATCAGCAGA ACATTCACTT 
TCTATTGGGT CCTTGCTGGA CATTTCTAAC ACTCCAGAGT CAAGCACTAT CAGTGGAGAA 
ACACCAAAAT CCTGTGCAAG GCCTTCCAAA AATTCAACTC CTCTTCCAGT AAAGCAGTCT 
GCAAGATGGC AGGTTGCAAA GGAATTGTAT CAGACAGAAA GTAACTATGT TGACATTCTA 
ACAACAATCA TTCAGTTATT TCAAGTTCCA TTGGAGAAGG AAGGACAACT TGGAGGACCA 
ATCCTTGCAC AGGAAGAGAT TAAGACAATA TTTGGCAGCA TTCCAGATAT TCTTGATGTG 
CATACTAAAA TCAAGGAAGA TTTGGAAGAT CTTATGATTA ATTGGACTGA AAGCAAGAGT 
ATTGGTGATA TAATTCTTAA ATATTCAAAA GACTTGTTGA AAACATACCC TCCATTTGTG 
AATTTCTTTG AAATGAGCAA GGAGACTATT ACTAGATGTG AAAAACAGAA GCCAAGGTTT 
CATGCATTCC TAAAGATAAA CCAAGCTAAA CCTGAATGTG GCCGCCAAAG CCTAGCTGAA 
CTTCTCATCC GTCCTGTTCA AAGGCTGCCT AGTGTGGCTC TCTTACTAAA TGATATTAAG 
AAGCATACAG CAGAAGAGAA CCCAGATAAA ATAACTCTAG AGAGAGCTAT TGAATCATTA 
AAGGAGGTGA TGACACATAT TAATGAAGAC AAAAGAAAAA CAGAGGCACA AAGGCAGTTC 
TTTGATGTTG TCTATGAAGT TGATGGATGT CCAGCCAATC TCTTGTCCTC TCATCGAAGC 
TTAGTTCAGC GTTTGGAAAC CATAGCACTC GGCGATGACC TCTGTGACAG GGGAGAACAG 
GTCACACTCT TGTTCAATGA TTGCCTAGAG ATTGCGAGGA AACGGCATAA AGTTATTGGT 
GCCTTCAAGA GTCCGCATGG CCACACAAGG CCTCCTGCAT CTCTCAAACA TGTTGTTCTC 
ATGCCTCTCT CTCAGATCAA GAAAGTCTTA GACATCAGGG AAACAGAAGA TTGCCATAAA 
GCTTTTGCCT TAGTTGTGCG GCCACCTACA GAACTCAACA ACAAGCTACT CAGTTTCCAG 
ATGACAACTG AAGAACCTCC TAAAGAAGAT TGGTTGAAGA TGTTGTGTCG GCATGTGGCG 
AACACTATTT GTAAAGCGGA TGCAGAAAAC CTCATTTACG TTGCTGATCC TGATTCAGTT 
GAGGTAAATA CTAAAGATAT GGACAGTACT TTAAGCAGAG CATCCAGAGC AATAAAGAAA 
ACATCAAAAA AGGTTACAAG AGCATTTTCT TTCACAAAAA CACCAAAGAG AGCTCTTCGA 
AGGGCTTTAA TGTCGCACAG TGCAACAGAA GGAAGAAGTC CCAGCTCAGC TAATGAGAGC 
TGTATAGGCA GCCGACTGAC TAGTGCGTCG TCATTAGCGG GTATTCCTTC TCCGTCCTTG 
GTCAGTCTTC CTTCAATCTT TGAAAAAAGG AGTCATACAT TAAGTCGTTC AACAACCCAC 
TTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010190349.1
CDS	1..2649
Translation	MSDNSLVVSETGRSLLADSSVLDSKIIETSKENALPVSVLDVEEEMPQIETRVVLVQEAGKHEELLKALEEIKVPYIKTDTIEELGDSDSPEFETIFVVSDFQDSIFNNLCKADCRVIGPPVVLHCAQKGEPLPFSCRPLYCACMLNLVLCFTGFRKKDELVNLVTLVHHMGGIIRKDFSSKVTHLVANSTHGDKFRIAVSLGTPIVKAEWIYKAWEKRNEINFCAAEDYFRNQFKVLPFQDCMLSFLGFSDDEKANMEEMTEMQGGCYLPVGDERCTHLVVEESTVKDLPFEPLKKLYVVKQEWFWGSIQMDARAGESMYLFEKSESPGLKKSVSLLSLNTPNSNRKRRRLKETLAQLTRETDMSPFPPRKRPSAEHSLSIGSLLDISNTPESSTISGETPKSCARPSKNSTPLPVKQSARWQVAKELYQTESNYVDILTTIIQLFQVPLEKEGQLGGPILAQEEIKTIFGSIPDILDVHTKIKEDLEDLMINWTESKSIGDIILKYSKDLLKTYPPFVNFFEMSKETITRCEKQKPRFHAFLKINQAKPECGRQSLAELLIRPVQRLPSVALLLNDIKKHTAEENPDKITLERAIESLKEVMTHINEDKRKTEAQRQFFDVVYEVDGCPANLLSSHRSLVQRLETIALGDDLCDRGEQVTLLFNDCLEIARKRHKVIGAFKSPHGHTRPPASLKHVVLMPLSQIKKVLDIRETEDCHKAFALVVRPPTELNNKLLSFQMTTEEPPKEDWLKMLCRHVANTICKADAENLIYVADPDSVEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFTKTPKRALRRALMSHSATEGRSPSSANESCIGSRLTSASSLAGIPSPSLVSLPSIFEKRSHTLSRSTTHLI

Target ORF information:

RefSeq Version	XM_010192047.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010192047.1

ORF Insert Sequence:

ATGTCTGACA ACAGTTTGGT GGTGTCTGAA ACCGGGAGAA GCCTCTTGGC TGATTCTTCT 
GTTCTTGATT CAAAAATTAT AGAAACCTCC AAAGAGAATG CATTGCCTGT ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA GGAAGCAGGG 
AAACATGAGG AGCTTCTGAA AGCCTTGGAG GAAATTAAAG TGCCCTACAT AAAGACAGAC 
ACGATAGAAG AGCTTGGAGA TTCTGATTCC CCAGAATTTG AGACCATCTT TGTTGTGTCA 
GACTTCCAAG ACTCTATCTT CAACAACCTC TGCAAAGCAG ATTGCCGTGT CATTGGACCT 
CCAGTAGTAC TGCACTGTGC ACAAAAAGGA GAGCCTTTAC CTTTCTCCTG TCGTCCACTG 
TACTGTGCAT GTATGTTGAA CTTGGTGCTG TGCTTTACGG GATTCAGAAA GAAGGATGAA 
TTAGTTAATC TCGTGACCTT AGTTCATCAT ATGGGTGGAA TTATTCGAAA GGACTTCAGC 
TCGAAAGTTA CACATCTGGT GGCTAACTCA ACACATGGAG ACAAATTCAG AATTGCTGTA 
AGCCTTGGTA CTCCTATCGT GAAAGCTGAG TGGATTTATA AAGCTTGGGA GAAAAGGAAT 
GAAATTAATT TCTGTGCAGC TGAAGATTAC TTTAGAAATC AGTTCAAGGT TCTTCCCTTC 
CAGGATTGCA TGTTAAGTTT CCTTGGATTC TCTGATGATG AGAAAGCTAA TATGGAAGAA 
ATGACAGAAA TGCAAGGAGG ATGTTATTTA CCAGTTGGTG ATGAAAGGTG TACACACTTA 
GTGGTTGAAG AGAGTACAGT AAAAGATCTT CCATTTGAAC CTTTAAAAAA ACTTTATGTT 
GTAAAGCAAG AGTGGTTCTG GGGAAGCATA CAAATGGATG CCAGAGCTGG AGAGTCCATG 
TATTTATTTG AGAAGTCAGA AAGTCCTGGC CTCAAGAAGT CTGTGTCACT ACTTTCTCTG 
AATACTCCAA ACAGCAATCG CAAAAGACGT CGTTTGAAAG AGACCCTTGC ACAACTGACC 
AGAGAAACAG ATATGTCACC ATTCCCTCCT CGGAAACGCC CATCAGCAGA ACATTCACTT 
TCTATTGGGT CCTTGCTGGA CATTTCTAAC ACTCCAGAGT CAAGCACTAT CAGTGGAGAA 
ACACCAAAAT CCTGTGCAAG GCCTTCCAAA AATTCAACTC CTCTTCCAGT AAAGCAGTCT 
GCAAGATGGC AGGTTGCAAA GGAATTGTAT CAGACAGAAA GTAACTATGT TGACATTCTA 
ACAACAATCA TTCAGTTATT TCAAGTTCCA TTGGAGAAGG AAGGACAACT TGGAGGACCA 
ATCCTTGCAC AGGAAGAGAT TAAGACAATA TTTGGCAGCA TTCCAGATAT TCTTGATGTG 
CATACTAAAA TCAAGGAAGA TTTGGAAGAT CTTATGATTA ATTGGACTGA AAGCAAGAGT 
ATTGGTGATA TAATTCTTAA ATATTCAAAA GACTTGTTGA AAACATACCC TCCATTTGTG 
AATTTCTTTG AAATGAGCAA GGAGACTATT ACTAGATGTG AAAAACAGAA GCCAAGGTTT 
CATGCATTCC TAAAGATAAA CCAAGCTAAA CCTGAATGTG GCCGCCAAAG CCTAGCTGAA 
CTTCTCATCC GTCCTGTTCA AAGGCTGCCT AGTGTGGCTC TCTTACTAAA TGATATTAAG 
AAGCATACAG CAGAAGAGAA CCCAGATAAA ATAACTCTAG AGAGAGCTAT TGAATCATTA 
AAGGAGGTGA TGACACATAT TAATGAAGAC AAAAGAAAAA CAGAGGCACA AAGGCAGTTC 
TTTGATGTTG TCTATGAAGT TGATGGATGT CCAGCCAATC TCTTGTCCTC TCATCGAAGC 
TTAGTTCAGC GTTTGGAAAC CATAGCACTC GGCGATGACC TCTGTGACAG GGGAGAACAG 
GTCACACTCT TGTTCAATGA TTGCCTAGAG ATTGCGAGGA AACGGCATAA AGTTATTGGT 
GCCTTCAAGA GTCCGCATGG CCACACAAGG CCTCCTGCAT CTCTCAAACA TGTTGTTCTC 
ATGCCTCTCT CTCAGATCAA GAAAGTCTTA GACATCAGGG AAACAGAAGA TTGCCATAAA 
GCTTTTGCCT TAGTTGTGCG GCCACCTACA GAACTCAACA ACAAGCTACT CAGTTTCCAG 
ATGACAACTG AAGAACCTCC TAAAGAAGAT TGGTTGAAGA TGTTGTGTCG GCATGTGGCG 
AACACTATTT GTAAAGCGGA TGCAGAAAAC CTCATTTACG TTGCTGATCC TGATTCAGTT 
GAGGTAAATA CTAAAGATAT GGACAGTACT TTAAGCAGAG CATCCAGAGC AATAAAGAAA 
ACATCAAAAA AGGTTACAAG AGCATTTTCT TTCACAAAAA CACCAAAGAG AGCTCTTCGA 
AGGGCTTTAA TGTCGCACAG TGCAACAGAA GGAAGAAGTC CCAGCTCAGC TAATGAGAGC 
TGTATAGGCA GCCGACTGAC TAGTGCGTCG TCATTAGCGG GTATTCCTTC TCCGTCCTTG 
GTCAGTCTTC CTTCAATCTT TGAAAAAAGG AGTCATACAT TAAGTCGTTC AACAACCCAC 
TTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECT2 ( XM_010192048.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010190350.1 Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe84071
Clone ID Related Accession (Same CDS sequence)	XM_010192048.1
Accession Version	XM_010192048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	protein ECT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010207202.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA ACAGTTTGGT GGTGTCTGAA ACCGGGAGAA GCCTCTTGGC TGATTCTTCT 
GTTCTTGATT CAAAAATTAT AGAAACCTCC AAAGAGAATG CATTGCCTGT ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA GGAAGCAGGG 
AAACATGAGG AGCTTCTGAA AGCCTTGGAG ACCATTAAGA TAATGGAAGT ACCAGTTATA 
AGGATAAAGA AGACTAGCCC TGGTAAATCA GAAGAAAAAC TAATAAAAAG TATTATTCAT 
ATGGAAATTA AAGTGCCCTA CATAAAGACA GACACGATAG AAGAGCTTGG AGATTCTGAT 
TCCCCAGAAT TTGAGACCAT CTTTGTTGTG TCAGACTTCC AAGACTCTAT CTTCAACAAC 
CTCTGCAAAG CAGATTGCCG TGTCATTGGA CCTCCAGTAG TACTGCACTG TGCACAAAAA 
GGAGAGCCTT TACCTTTCTC CTGTCGTCCA CTGTACTGTG CATGTATGTT GAACTTGGTG 
CTGTGCTTTA CGGGATTCAG AAAGAAGGAT GAATTAGTTA ATCTCGTGAC CTTAGTTCAT 
CATATGGGTG GAATTATTCG AAAGGACTTC AGCTCGAAAG TTACACATCT GGTGGCTAAC 
TCAACACATG GAGACAAATT CAGAATTGCT GTAAGCCTTG GTACTCCTAT CGTGAAAGCT 
GAGTGGATTT ATAAAGCTTG GGAGAAAAGG AATGAAATTA ATTTCTGTGC AGCTGAAGAT 
TACTTTAGAA ATCAGTTCAA GGTTCTTCCC TTCCAGGATT GCATGTTAAG TTTCCTTGGA 
TTCTCTGATG ATGAGAAAGC TAATATGGAA GAAATGACAG AAATGCAAGG AGGATGTTAT 
TTACCAGTTG GTGATGAAAG GTGTACACAC TTAGTGGTTG AAGAGAGTAC AGTAAAAGAT 
CTTCCATTTG AACCTTTAAA AAAACTTTAT GTTGTAAAGC AAGAGTGGTT CTGGGGAAGC 
ATACAAATGG ATGCCAGAGC TGGAGAGTCC ATGTATTTAT TTGAGAAGTC AGAAAGTCCT 
GGCCTCAAGA AGTCTGTGTC ACTACTTTCT CTGAATACTC CAAACAGCAA TCGCAAAAGA 
CGTCGTTTGA AAGAGACCCT TGCACAACTG ACCAGAGAAA CAGATATGTC ACCATTCCCT 
CCTCGGAAAC GCCCATCAGC AGAACATTCA CTTTCTATTG GGTCCTTGCT GGACATTTCT 
AACACTCCAG AGTCAAGCAC TATCAGTGGA GAAACACCAA AATCCTGTGC AAGGCCTTCC 
AAAAATTCAA CTCCTCTTCC AGTAAAGCAG TCTGCAAGAT GGCAGGTTGC AAAGGAATTG 
TATCAGACAG AAAGTAACTA TGTTGACATT CTAACAACAA TCATTCAGTT ATTTCAAGTT 
CCATTGGAGA AGGAAGGACA ACTTGGAGGA CCAATCCTTG CACAGGAAGA GATTAAGACA 
ATATTTGGCA GCATTCCAGA TATTCTTGAT GTGCATACTA AAATCAAGGA AGATTTGGAA 
GATCTTATGA TTAATTGGAC TGAAAGCAAG AGTATTGGTG ATATAATTCT TAAATATTCA 
AAAGACTTGT TGAAAACATA CCCTCCATTT GTGAATTTCT TTGAAATGAG CAAGGAGACT 
ATTACTAGAT GTGAAAAACA GAAGCCAAGG TTTCATGCAT TCCTAAAGAT AAACCAAGCT 
AAACCTGAAT GTGGCCGCCA AAGCCTAGCT GAACTTCTCA TCCGTCCTGT TCAAAGGCTG 
CCTAGTGTGG CTCTCTTACT AAATGATATT AAGAAGCATA CAGCAGAAGA GAACCCAGAT 
AAAATAACTC TAGAGAGAGC TATTGAATCA TTAAAGGAGG TGATGACACA TATTAATGAA 
GACAAAAGAA AAACAGAGGC ACAAAGGCAG TTCTTTGATG TTGTCTATGA AGTTGATGGA 
TGTCCAGCCA ATCTCTTGTC CTCTCATCGA AGCTTAGTTC AGCGTTTGGA AACCATAGCA 
CTCGGCGATG ACCTCTGTGA CAGGGGAGAA CAGGTCACAC TCTTGTTCAA TGATTGCCTA 
GAGATTGCGA GGAAACGGCA TAAAGTTATT GGTGCCTTCA AGAGTCCGCA TGGCCACACA 
AGGCCTCCTG CATCTCTCAA ACATGTTGTT CTCATGCCTC TCTCTCAGAT CAAGAAAGTC 
TTAGACATCA GGGAAACAGA AGATTGCCAT AAAGCTTTTG CCTTAGTTGT GCGGCCACCT 
ACAGAACTCA ACAACAAGCT ACTCAGTTTC CAGATGACAA CTGAAGAACC TCCTAAAGAA 
GATTGGTTGA AGATGTTGTG TCGGCATGTG GCGAACACTA TTTGTAAAGC GGATGCAGAA 
AACCTCATTT ACGTTGCTGA TCCTGATTCA GTTGAGGTAA ATACTAAAGA TATGGACAGT 
ACTTTAAGCA GAGCATCCAG AGCAATAAAG AAAACATCAA AAAAGGTTAC AAGAGCATTT 
TCTTTCACAA AAACACCAAA GAGAGCTCTT CGAAGGGCTT TAATGTCGCA CAGTGCAACA 
GAAGGAAGAA GTCCCAGCTC AGCTAATGAG AGCTGTATAG GCAGCCGACT GACTAGTGCG 
TCGTCATTAG CGGGTATTCC TTCTCCGTCC TTGGTCAGTC TTCCTTCAAT CTTTGAAAAA 
AGGAGTCATA CATTAAGTCG TTCAACAACC CACTTGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010190350.1
CDS	16..2757
Translation	MSDNSLVVSETGRSLLADSSVLDSKIIETSKENALPVSVLDVEEEMPQIETRVVLVQEAGKHEELLKALETIKIMEVPVIRIKKTSPGKSEEKLIKSIIHMEIKVPYIKTDTIEELGDSDSPEFETIFVVSDFQDSIFNNLCKADCRVIGPPVVLHCAQKGEPLPFSCRPLYCACMLNLVLCFTGFRKKDELVNLVTLVHHMGGIIRKDFSSKVTHLVANSTHGDKFRIAVSLGTPIVKAEWIYKAWEKRNEINFCAAEDYFRNQFKVLPFQDCMLSFLGFSDDEKANMEEMTEMQGGCYLPVGDERCTHLVVEESTVKDLPFEPLKKLYVVKQEWFWGSIQMDARAGESMYLFEKSESPGLKKSVSLLSLNTPNSNRKRRRLKETLAQLTRETDMSPFPPRKRPSAEHSLSIGSLLDISNTPESSTISGETPKSCARPSKNSTPLPVKQSARWQVAKELYQTESNYVDILTTIIQLFQVPLEKEGQLGGPILAQEEIKTIFGSIPDILDVHTKIKEDLEDLMINWTESKSIGDIILKYSKDLLKTYPPFVNFFEMSKETITRCEKQKPRFHAFLKINQAKPECGRQSLAELLIRPVQRLPSVALLLNDIKKHTAEENPDKITLERAIESLKEVMTHINEDKRKTEAQRQFFDVVYEVDGCPANLLSSHRSLVQRLETIALGDDLCDRGEQVTLLFNDCLEIARKRHKVIGAFKSPHGHTRPPASLKHVVLMPLSQIKKVLDIRETEDCHKAFALVVRPPTELNNKLLSFQMTTEEPPKEDWLKMLCRHVANTICKADAENLIYVADPDSVEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFTKTPKRALRRALMSHSATEGRSPSSANESCIGSRLTSASSLAGIPSPSLVSLPSIFEKRSHTLSRSTTHLI

Target ORF information:

RefSeq Version	XM_010192048.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010192048.1

ORF Insert Sequence:

ATGTCTGACA ACAGTTTGGT GGTGTCTGAA ACCGGGAGAA GCCTCTTGGC TGATTCTTCT 
GTTCTTGATT CAAAAATTAT AGAAACCTCC AAAGAGAATG CATTGCCTGT ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA GGAAGCAGGG 
AAACATGAGG AGCTTCTGAA AGCCTTGGAG ACCATTAAGA TAATGGAAGT ACCAGTTATA 
AGGATAAAGA AGACTAGCCC TGGTAAATCA GAAGAAAAAC TAATAAAAAG TATTATTCAT 
ATGGAAATTA AAGTGCCCTA CATAAAGACA GACACGATAG AAGAGCTTGG AGATTCTGAT 
TCCCCAGAAT TTGAGACCAT CTTTGTTGTG TCAGACTTCC AAGACTCTAT CTTCAACAAC 
CTCTGCAAAG CAGATTGCCG TGTCATTGGA CCTCCAGTAG TACTGCACTG TGCACAAAAA 
GGAGAGCCTT TACCTTTCTC CTGTCGTCCA CTGTACTGTG CATGTATGTT GAACTTGGTG 
CTGTGCTTTA CGGGATTCAG AAAGAAGGAT GAATTAGTTA ATCTCGTGAC CTTAGTTCAT 
CATATGGGTG GAATTATTCG AAAGGACTTC AGCTCGAAAG TTACACATCT GGTGGCTAAC 
TCAACACATG GAGACAAATT CAGAATTGCT GTAAGCCTTG GTACTCCTAT CGTGAAAGCT 
GAGTGGATTT ATAAAGCTTG GGAGAAAAGG AATGAAATTA ATTTCTGTGC AGCTGAAGAT 
TACTTTAGAA ATCAGTTCAA GGTTCTTCCC TTCCAGGATT GCATGTTAAG TTTCCTTGGA 
TTCTCTGATG ATGAGAAAGC TAATATGGAA GAAATGACAG AAATGCAAGG AGGATGTTAT 
TTACCAGTTG GTGATGAAAG GTGTACACAC TTAGTGGTTG AAGAGAGTAC AGTAAAAGAT 
CTTCCATTTG AACCTTTAAA AAAACTTTAT GTTGTAAAGC AAGAGTGGTT CTGGGGAAGC 
ATACAAATGG ATGCCAGAGC TGGAGAGTCC ATGTATTTAT TTGAGAAGTC AGAAAGTCCT 
GGCCTCAAGA AGTCTGTGTC ACTACTTTCT CTGAATACTC CAAACAGCAA TCGCAAAAGA 
CGTCGTTTGA AAGAGACCCT TGCACAACTG ACCAGAGAAA CAGATATGTC ACCATTCCCT 
CCTCGGAAAC GCCCATCAGC AGAACATTCA CTTTCTATTG GGTCCTTGCT GGACATTTCT 
AACACTCCAG AGTCAAGCAC TATCAGTGGA GAAACACCAA AATCCTGTGC AAGGCCTTCC 
AAAAATTCAA CTCCTCTTCC AGTAAAGCAG TCTGCAAGAT GGCAGGTTGC AAAGGAATTG 
TATCAGACAG AAAGTAACTA TGTTGACATT CTAACAACAA TCATTCAGTT ATTTCAAGTT 
CCATTGGAGA AGGAAGGACA ACTTGGAGGA CCAATCCTTG CACAGGAAGA GATTAAGACA 
ATATTTGGCA GCATTCCAGA TATTCTTGAT GTGCATACTA AAATCAAGGA AGATTTGGAA 
GATCTTATGA TTAATTGGAC TGAAAGCAAG AGTATTGGTG ATATAATTCT TAAATATTCA 
AAAGACTTGT TGAAAACATA CCCTCCATTT GTGAATTTCT TTGAAATGAG CAAGGAGACT 
ATTACTAGAT GTGAAAAACA GAAGCCAAGG TTTCATGCAT TCCTAAAGAT AAACCAAGCT 
AAACCTGAAT GTGGCCGCCA AAGCCTAGCT GAACTTCTCA TCCGTCCTGT TCAAAGGCTG 
CCTAGTGTGG CTCTCTTACT AAATGATATT AAGAAGCATA CAGCAGAAGA GAACCCAGAT 
AAAATAACTC TAGAGAGAGC TATTGAATCA TTAAAGGAGG TGATGACACA TATTAATGAA 
GACAAAAGAA AAACAGAGGC ACAAAGGCAG TTCTTTGATG TTGTCTATGA AGTTGATGGA 
TGTCCAGCCA ATCTCTTGTC CTCTCATCGA AGCTTAGTTC AGCGTTTGGA AACCATAGCA 
CTCGGCGATG ACCTCTGTGA CAGGGGAGAA CAGGTCACAC TCTTGTTCAA TGATTGCCTA 
GAGATTGCGA GGAAACGGCA TAAAGTTATT GGTGCCTTCA AGAGTCCGCA TGGCCACACA 
AGGCCTCCTG CATCTCTCAA ACATGTTGTT CTCATGCCTC TCTCTCAGAT CAAGAAAGTC 
TTAGACATCA GGGAAACAGA AGATTGCCAT AAAGCTTTTG CCTTAGTTGT GCGGCCACCT 
ACAGAACTCA ACAACAAGCT ACTCAGTTTC CAGATGACAA CTGAAGAACC TCCTAAAGAA 
GATTGGTTGA AGATGTTGTG TCGGCATGTG GCGAACACTA TTTGTAAAGC GGATGCAGAA 
AACCTCATTT ACGTTGCTGA TCCTGATTCA GTTGAGGTAA ATACTAAAGA TATGGACAGT 
ACTTTAAGCA GAGCATCCAG AGCAATAAAG AAAACATCAA AAAAGGTTAC AAGAGCATTT 
TCTTTCACAA AAACACCAAA GAGAGCTCTT CGAAGGGCTT TAATGTCGCA CAGTGCAACA 
GAAGGAAGAA GTCCCAGCTC AGCTAATGAG AGCTGTATAG GCAGCCGACT GACTAGTGCG 
TCGTCATTAG CGGGTATTCC TTCTCCGTCC TTGGTCAGTC TTCCTTCAAT CTTTGAAAAA 
AGGAGTCATA CATTAAGTCG TTCAACAACC CACTTGATAT GA