EEF2 cDNA ORF clone, Mesitornis unicolor(brown roatelo)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF2
Entrez Gene ID	104544388
Full Name	eukaryotic translation elongation factor 2
Gene Type	protein-coding
Organism	Mesitornis unicolor(brown roatelo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010189248.1	XP_010187550.1	elongation factor 2

Pathways from BioSystems
Gene Ontology

The following EEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe82758	XM_010189248.1 Latest version!	Mesitornis unicolor eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2 ( XM_010189248.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010187550.1 Mesitornis unicolor eukaryotic translation elongation factor 2 (EEF2), mRNA.

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CloneID	OMe82758
Clone ID Related Accession (Same CDS sequence)	XM_010189248.1
Accession Version	XM_010189248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	elongation factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010198724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAAA AGGCCAACAT CAGAAACATG TCTGTGATTG CCCACGTTGA TCACGACAAA 
TCAACCTTGA CTGATTCTCT GGTATGCAAA GCTGGTATCA TCGCCTCTGC CCGCGCGGGG 
GAGACCCGTT TCACCGACAC AAGGAAGGAT GAGCAGGAGC GGTGCATTAC CATCAAATCA 
ACAGCTATTT CTCTATTTTA TGAACTCTCT GAAAACGATT TGGCCTTCAT CAAGCAGAGC 
AAGGATGGTT CTGGTTTCTT GATCAACCTC ATTGACTCTC CTGGTCACGT AGACTTCTCT 
TCAGAGGTCA CTGCTGCTCT TCGTGTCACT GATGGTGCCC TGGTTGTTGT AGACTGTGTC 
TCTGGCGTGT GTGTGCAGAC AGAGACTGTG CTGCGTCAGG CCATTGCCGA GAGGATCAAG 
CCTGTCCTGA TGATGAACAA GATGGACCGA GCGCTGCTGG AACTGCAGCT GGAGCCAGAA 
GAGCTGTACC AGACCTTCCA GCGCATTGTG GAAAACGTGA ACGTCATCAT CTCCACGTAT 
GGAGAAGGAG AAAGTGGCCC CATGGGAAAC ATCATGATTG ATCCAGTGCT TGGTACTGTC 
GGCTTTGGTT CTGGCCTGCA CGGCTGGGCT TTCACTCTGA AACAGTTTGC GGAAATGTAC 
GTTGCAAAGT TTGCTGCCAA GGGCGATGCC CAGCTGAATC CATCCGAGCG TGCCAAGAAA 
GTAGAAGACA TGATGAAGAA GCTGTGGGGA GACAGATATT TTGATCCTGC TACTGGCAAG 
TTCAGCAAAT CTGCTACCAG CCCTGATGGA AAGAAACTGC CCAGAACGTT CTGCCAGCTC 
ATCCTTGACC CCATCTTCAA GGTTTTTGAT GCAATCATGA CCTTCAAGAA AGAGGAGGCG 
GCTAAACTGA TTGAGAAACT GGACATCAAG CTTGACAGTG AAGATAAGGA CAAGGAGGGC 
AAACCCCTGC TGAAGGCCGT GATGAGGCGG TGGCTGCCTG CTGGAGATGC CCTGCTGCAG 
ATGATCACCA TTCACCTGCC TTCTCCTGTC ACAGCCCAGA AGTACCGCTG TGAGCTGCTC 
TACGAGGGAC CCCCTGATGA TGAGGCTGCC ATAGGTATTA AGAACTGTGA CCCCAAAGGC 
CCCCTGATGA TGTACATCTC TAAAATGGTG CCAACCTCTG ACAAGGGACG TTTCTACGCT 
TTTGGACGTG TCTTCTCTGG TCTCGTTTCA ACTGGCTTGA AAGTCAGAAT CATGGGACCA 
AACTACACCC CTGGCAAGAA GGAAGATCTG TACCTGAAGC CAATTCAAAG GACCATTCTG 
ATGATGGGCC GCTATGTTGA ACCCATCGAG GACGTGCCTT GTGGAAACAT TGTTGGTCTG 
GTTGGCGTCG ACCAGTTCCT TGTGAAGACT GGAACCATCA CCACCTTTGA GCACGCTCAC 
AACATGAGAG TCATGAAGTT CAGTGTCAGC CCCGTTGTGC GCGTGGCTGT GGAAGCCAAG 
AACCCAGCTG ACCTGCCCAA GCTGGTTGAG GGCCTGAAGC GTCTTGCCAA GTCTGACCCT 
ATGGTGCAGT GCATCATTGA GGAGTCTGGG GAACACATCA TTGCTGGGGC AGGGGAGCTG 
CATCTGGAGA TCTGCCTGAA GGATTTGGAA GAGGACCACG CGTGCATCCC TATTAAGAAA 
TCAGATCCTG TTGTGTCCTA CCGGGAGACA GTCAGCGAGG AGTCCAGTGT GATGTGCCTT 
TCCAAGTCCC CCAACAAACA CAACAGGCTG TACATGAAGG CCCGGCCCTT CCCAGATGGT 
TTGGCCGAAG ACATTGACAA GGGTGAGGTC TCTGCCCGTC AGGAGCTGAA GCAGCGAGCT 
CGTTACCTGG CTGAGAAGTA CGAGTGGGAT GTCACCGAAG CCAGGAAAAT CTGGTGCTTT 
GGTCCTGACG GCACTGGCCC CAACATCCTG ACTGACATCA CCAAGGGAGT GCAGTACCTG 
AATGAGATCA AGGACAGCGT GGTGGCTGGC TTCCAGTGGG CAACAAAGGA GGGGGTCCTG 
TGTGAGGAGA ACATGCGTGG TGTGCGTTTT GATGTGCACG ATGTCACCTT GCACGCTGAT 
GCCATCCACC GTGGAGGTGG GCAGATCATT CCAACTGCCA GGAGATGCCT GTACGCCTGT 
GTGCTCACCG CTCAGCCCAG GCTCATGGAG CCCATCTACC TTGTGGAGAT CCAGTGCCCA 
GAGCAGGTGG TGGGTGGCAT CTACGGTGTG CTGAACAGGA AACGTGGCCA CGTGTTTGAG 
GAGACCCAGG TGGCTGGCAC CCCCATGTTC GTGGTCAAGG CGTACCTGCC CGTCAACGAG 
TCCTTTGGTT TCACAGCAGA TTTGAGATCC AACACAGGGG GCCAGGCCTT CCCCCAGTGT 
GTGTTCGACC ACTGGCAGAT CCTGCCCGGG GATCCCTTTG ACAGCGCCAG CCGCCCCTGC 
ACCGTGGTGA ATGAGACCCG CAAACGCAAA GGGCTGAAGG AAGGCATCCC TGCTCTTGAC 
AACTTCCTTG ACAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010187550.1
CDS	50..2590
Translation	MDKKANIRNMSVIAHVDHDKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLAFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGDAQLNPSERAKKVEDMMKKLWGDRYFDPATGKFSKSATSPDGKKLPRTFCQLILDPIFKVFDAIMTFKKEEAAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAIGIKNCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESSVMCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVTEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGVLCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYACVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEETQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSASRPCTVVNETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	XM_010189248.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010189248.1

ORF Insert Sequence:

ATGGACAAAA AGGCCAACAT CAGAAACATG TCTGTGATTG CCCACGTTGA TCACGACAAA 
TCAACCTTGA CTGATTCTCT GGTATGCAAA GCTGGTATCA TCGCCTCTGC CCGCGCGGGG 
GAGACCCGTT TCACCGACAC AAGGAAGGAT GAGCAGGAGC GGTGCATTAC CATCAAATCA 
ACAGCTATTT CTCTATTTTA TGAACTCTCT GAAAACGATT TGGCCTTCAT CAAGCAGAGC 
AAGGATGGTT CTGGTTTCTT GATCAACCTC ATTGACTCTC CTGGTCACGT AGACTTCTCT 
TCAGAGGTCA CTGCTGCTCT TCGTGTCACT GATGGTGCCC TGGTTGTTGT AGACTGTGTC 
TCTGGCGTGT GTGTGCAGAC AGAGACTGTG CTGCGTCAGG CCATTGCCGA GAGGATCAAG 
CCTGTCCTGA TGATGAACAA GATGGACCGA GCGCTGCTGG AACTGCAGCT GGAGCCAGAA 
GAGCTGTACC AGACCTTCCA GCGCATTGTG GAAAACGTGA ACGTCATCAT CTCCACGTAT 
GGAGAAGGAG AAAGTGGCCC CATGGGAAAC ATCATGATTG ATCCAGTGCT TGGTACTGTC 
GGCTTTGGTT CTGGCCTGCA CGGCTGGGCT TTCACTCTGA AACAGTTTGC GGAAATGTAC 
GTTGCAAAGT TTGCTGCCAA GGGCGATGCC CAGCTGAATC CATCCGAGCG TGCCAAGAAA 
GTAGAAGACA TGATGAAGAA GCTGTGGGGA GACAGATATT TTGATCCTGC TACTGGCAAG 
TTCAGCAAAT CTGCTACCAG CCCTGATGGA AAGAAACTGC CCAGAACGTT CTGCCAGCTC 
ATCCTTGACC CCATCTTCAA GGTTTTTGAT GCAATCATGA CCTTCAAGAA AGAGGAGGCG 
GCTAAACTGA TTGAGAAACT GGACATCAAG CTTGACAGTG AAGATAAGGA CAAGGAGGGC 
AAACCCCTGC TGAAGGCCGT GATGAGGCGG TGGCTGCCTG CTGGAGATGC CCTGCTGCAG 
ATGATCACCA TTCACCTGCC TTCTCCTGTC ACAGCCCAGA AGTACCGCTG TGAGCTGCTC 
TACGAGGGAC CCCCTGATGA TGAGGCTGCC ATAGGTATTA AGAACTGTGA CCCCAAAGGC 
CCCCTGATGA TGTACATCTC TAAAATGGTG CCAACCTCTG ACAAGGGACG TTTCTACGCT 
TTTGGACGTG TCTTCTCTGG TCTCGTTTCA ACTGGCTTGA AAGTCAGAAT CATGGGACCA 
AACTACACCC CTGGCAAGAA GGAAGATCTG TACCTGAAGC CAATTCAAAG GACCATTCTG 
ATGATGGGCC GCTATGTTGA ACCCATCGAG GACGTGCCTT GTGGAAACAT TGTTGGTCTG 
GTTGGCGTCG ACCAGTTCCT TGTGAAGACT GGAACCATCA CCACCTTTGA GCACGCTCAC 
AACATGAGAG TCATGAAGTT CAGTGTCAGC CCCGTTGTGC GCGTGGCTGT GGAAGCCAAG 
AACCCAGCTG ACCTGCCCAA GCTGGTTGAG GGCCTGAAGC GTCTTGCCAA GTCTGACCCT 
ATGGTGCAGT GCATCATTGA GGAGTCTGGG GAACACATCA TTGCTGGGGC AGGGGAGCTG 
CATCTGGAGA TCTGCCTGAA GGATTTGGAA GAGGACCACG CGTGCATCCC TATTAAGAAA 
TCAGATCCTG TTGTGTCCTA CCGGGAGACA GTCAGCGAGG AGTCCAGTGT GATGTGCCTT 
TCCAAGTCCC CCAACAAACA CAACAGGCTG TACATGAAGG CCCGGCCCTT CCCAGATGGT 
TTGGCCGAAG ACATTGACAA GGGTGAGGTC TCTGCCCGTC AGGAGCTGAA GCAGCGAGCT 
CGTTACCTGG CTGAGAAGTA CGAGTGGGAT GTCACCGAAG CCAGGAAAAT CTGGTGCTTT 
GGTCCTGACG GCACTGGCCC CAACATCCTG ACTGACATCA CCAAGGGAGT GCAGTACCTG 
AATGAGATCA AGGACAGCGT GGTGGCTGGC TTCCAGTGGG CAACAAAGGA GGGGGTCCTG 
TGTGAGGAGA ACATGCGTGG TGTGCGTTTT GATGTGCACG ATGTCACCTT GCACGCTGAT 
GCCATCCACC GTGGAGGTGG GCAGATCATT CCAACTGCCA GGAGATGCCT GTACGCCTGT 
GTGCTCACCG CTCAGCCCAG GCTCATGGAG CCCATCTACC TTGTGGAGAT CCAGTGCCCA 
GAGCAGGTGG TGGGTGGCAT CTACGGTGTG CTGAACAGGA AACGTGGCCA CGTGTTTGAG 
GAGACCCAGG TGGCTGGCAC CCCCATGTTC GTGGTCAAGG CGTACCTGCC CGTCAACGAG 
TCCTTTGGTT TCACAGCAGA TTTGAGATCC AACACAGGGG GCCAGGCCTT CCCCCAGTGT 
GTGTTCGACC ACTGGCAGAT CCTGCCCGGG GATCCCTTTG ACAGCGCCAG CCGCCCCTGC 
ACCGTGGTGA ATGAGACCCG CAAACGCAAA GGGCTGAAGG AAGGCATCCC TGCTCTTGAC 
AACTTCCTTG ACAAACTCTA A