LOC100303687 cDNA ORF clone, Meleagris gallopavo(turkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100303687
Entrez Gene ID	100303687
Full Name	interleukin-4
Synonyms	IL-13
Gene Type	protein-coding
Organism	Meleagris gallopavo(turkey)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001303181.1	NP_001290110.1	interleukin-4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13.
Powell F, Rothwell L, Clarkson M, Kaiser P
Veterinary immunology and immunopathology147(1-2)97-103(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively
Title: Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13.

The following LOC100303687 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100303687 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe44342	NM_001303181.1 Latest version!	Meleagris gallopavo interleukin-4 (LOC100303687), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100303687 ( NM_001303181.1 ) cDNA ORF clone, Meleagris gallopavo(turkey) -> NP_001290110.1 Meleagris gallopavo interleukin-4 (LOC100303687), mRNA.

Price & Availability↑

CloneID	OMe44342
Clone ID Related Accession (Same CDS sequence)	NM_001303181.1
Accession Version	NM_001303181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-14
Organism	Meleagris gallopavo(turkey)
Product	interleukin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM493431.1. On Dec 19, 2014 this sequence version replaced XM_003210483.2. ##Evidence-Data-START## Transcript exon combination :: AM493431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03010136, SAMN03010138 [ECO:0000348] ##Evidence-Data-END##

ATGCACCGCA CACTGAAGGC TGCCCTTGCT CTCCTCTGTC TGGCAGAGCT CATTGCCTCC 
ACACCGCTGC CGACGAGCTC CATGCCCAAG ATGAAGCTGT CGGACATCAC TCGAAGCATC 
CAGCAGCTCA ACAAAGGAGA GCAGGTCCCT TGCAATGACA CCAGAGTGGC ACAGGTGGCT 
TTCACGGATC GGAAGCTGTC GGAGCAGGAA CTGCTGTGCC AGGCTGCCAC AGTGCTGACC 
AAGGTGACCC GCTGCAAGAA GGACTACGAG CCCCTCATCA CCAGTCTGCA GAGCCTGCAC 
GGCCAGATGA GCTGCCAGCT GAGCACCGAC AACGAGATCT ACCTGCGGAA CTTCTTGCCA 
ACGCTGGGGA ACTTCACCCA GGCGCTGTAC CGGCGGAACA CAGCCACAGC TGCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290110.1
CDS	1..420
Translation	MHRTLKAALALLCLAELIASTPLPTSSMPKMKLSDITRSIQQLNKGEQVPCNDTRVAQVAFTDRKLSEQELLCQAATVLTKVTRCKKDYEPLITSLQSLHGQMSCQLSTDNEIYLRNFLPTLGNFTQALYRRNTATAAN

Target ORF information:

RefSeq Version	NM_001303181.1
Organism	Meleagris gallopavo(turkey)
Definition	Meleagris gallopavo interleukin-4 (LOC100303687), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303181.1

ORF Insert Sequence:

ATGCACCGCA CACTGAAGGC TGCCCTTGCT CTCCTCTGTC TGGCAGAGCT CATTGCCTCC 
ACACCGCTGC CGACGAGCTC CATGCCCAAG ATGAAGCTGT CGGACATCAC TCGAAGCATC 
CAGCAGCTCA ACAAAGGAGA GCAGGTCCCT TGCAATGACA CCAGAGTGGC ACAGGTGGCT 
TTCACGGATC GGAAGCTGTC GGAGCAGGAA CTGCTGTGCC AGGCTGCCAC AGTGCTGACC 
AAGGTGACCC GCTGCAAGAA GGACTACGAG CCCCTCATCA CCAGTCTGCA GAGCCTGCAC 
GGCCAGATGA GCTGCCAGCT GAGCACCGAC AACGAGATCT ACCTGCGGAA CTTCTTGCCA 
ACGCTGGGGA ACTTCACCCA GGCGCTGTAC CGGCGGAACA CAGCCACAGC TGCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development of reagents to study the turkey's immune response: cloning and characterisation of two turkey cytokines, interleukin (IL)-10 and IL-13.
Veterinary immunology and immunopathology147(1-2)97-103(2012 Jun)
Powell F,Rothwell L,Clarkson M,Kaiser P