LOC25501732 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25501732
Entrez Gene ID	25501732
Full Name	mannosyl-oligosaccharide glucosidase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013591101.1	XP_013446555.1	mannosyl-oligosaccharide glucosidase
XM_013591101.2	XP_013446555.1	mannosyl-oligosaccharide glucosidase GCS1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25501732 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25501732 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe166452	XM_013591101.2 Latest version!	Medicago truncatula mannosyl-oligosaccharide glucosidase GCS1 (LOC25501732), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMe166452	XM_013591101.1	Medicago truncatula mannosyl-oligosaccharide glucosidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25501732 ( XM_013591101.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013446555.1 Medicago truncatula mannosyl-oligosaccharide glucosidase mRNA.

Price & Availability↑

CloneID	OMe166452
Clone ID Related Accession (Same CDS sequence)	XM_013591101.1 , XM_013591101.2
Accession Version	XM_013591101.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	mannosyl-oligosaccharide glucosidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGACCGGAA GCGGAAGAAC AACCGCTCGG AGCAGAGTCA GATCTTCCTC TGAACCCGAC 
GCCGGCGAAG GAAGGCCGAA ATTTAACCGC CGCAGAGCCA CAAGCAAATA CGGAGGCAGC 
ATTCCGTTAT TCGACAATGT CAGCATCAAA ATTGTTCTCG CTATCAGCTT CATCGCATTC 
TTCATTATTT TGTTTCTCAT TCGTCACTTA GTTAATTCCG TTGCTGAACC TCGTCTTCCT 
CGCGCTGTTA CGCCGCTTCC CGCGCCAAAG ATCATGGACC TCGATCAGTT TCAAGGTGAG 
CACAAAGAGA GTTTGTATTG GGGAACATAT CGTCCTTATC TCTATCTCGG AATTCGTGCC 
AGGACTCCAC AATCTTTGAT GGCTGGATTA ATGTGGATTG GTGTGAAGGA CGGTAGATAT 
CACTTGAGGC ATGTTTGTAG ACACGAGGAT GACTTAAGTA CTTATGGTTG GAAAAAGCAT 
AATGGACGTG ATTATGGGCA CCAAGTACTA GTTGACCATG GCATGATATT GACTACCGAG 
TTTTTAAAAT CAAAGGGTGA TGACAGTGGT TATGGAGGGG ACTGGGCAGT TCGAATTGAT 
GTGCAAGTTG ATAAGTCTAA GTGGAATGAA GAATTTGGGA GAGGTGCCCA GCTCTTTTTC 
TATTTGGCCG ATGAAGGTGG TAATGTCCTA GATGTAAGTA GAGAAAACTT GGACAGTCGT 
GAGAGTTCTT TACTTGCATC TGGCTCTCGA TCAGACATTG GAGATTGGCA GCTACATTTA 
AAGTCCAAGG ATGATCTGAA ACTGCATTAT TCTGGATTTC ACACACCTCA CTTTCACAAT 
TTGTCTGATC TTGTTGAGGA AAATCTTGCA TCACAAATAA GAAAGCATGC TCAGCTGCAA 
CTATCTGACT CATCAGACGA ATCTTCTAAC GTGTTGGCTT TTCAGATTCT TGGTGGCTTT 
CCTTTCACTA CAGATATTGT GCTAATATCA GGAACTGATT CCGGAAGTTC AAGAGTGGAA 
GAGCGCATCA ATAGTCTTAC AGGAGCCTCA CTGTCCAATG AACTGAAATA TAAAGAACAA 
GCATTTGATG AAAAGATTGA GAAAGTTTTC AATTTGACAG AGAAGGTGGA CTCAGAATCC 
GTTTCTGCTG GTAAGGCTGC TATTGGAAGC TTATTAGGTG GCATCGGTTA CTTCTATGGC 
CAATCAAAAA TTGCTCTGTC AAGAACCCTT AATTTTAGGG AACATGTTGA TTATGTATCA 
TATTGGCCAG CTGAGCTTTA TACAGCTGTA CCAAGTCGGT CTTTCTTTCC AAGAGGATTT 
CTATGGGATG AAGGTTTTCA TCAACTTCTA ATCTGGCGTT GGGATATTCG TATTTCGTTG 
GATATCATCG GTCACTGGTT AGATTTGATG AACGTTGATG GATGGATACC ACGTGAACAA 
ATTCTGGGGG CTGAAGCATT AAGTAGGGTC CCAGATGAAT TTGTTCCTCA GCATCCAACA 
AATGGAAATC CTCCAACTAT GTTTTTAGCA TTAAGTGACA TAATTACTGG CTTGAAGAAT 
AATGAGTTCA CTGCAAAGGA TAGAAGTGAG ATCTCTATGT TCCTAGAACG CTCCTTTGTT 
CGATTAGAAG CATGGTTCCA ATGGTTCAAT ACAACTCAGT CAGGCAAACA AACGAGCAGC 
TACTACTGGC ATGGACGAGA TAACAGAACA ATGCGTGAAT TAAATCCCAA GTCACTGTCC 
TCTGGATTGG ATGATTACCC ACGTGCTTCA CACCCAAGTG AAGATGAACG CCACTTGGAT 
CTTAGATGCT GGATGTTACT AGCAGCAGAC TGTATGCATT CCATTGAGAA GTTGTTGGAT 
AAAGAAACCA GGCCTGGCAA GAATTATGGA TCTACTGCTA AATTGCTATC AGATCATGAG 
TTACTGAACC AGATGCATTT TGATGATGCT TATGGTGCTT ACTTTGATTT TGGAAATCAT 
ACAGAAAAGG CTCAACTAAA ATGGAAAGAG ATGGAGACTG GACAAAATTA TGCTTCTCGT 
CAGCTTGTTC GGGATGTTTC GAAGAGGCCT GTATTAAGAT TCGTTCCTCA CATTGGTTAT 
GTTAGTCTGT TCCCATTCAT GGGGAGAATC ATTCCACCTG GATCATGGAT TCTGGAAAAG 
CAGCTTGAAC TCATTTCAAA TAACAGCCTC TTATGGACTG ACTATGGACT ACGTTCTCTT 
GCCAAAACAA GTTCCATGTA CATGAAACGC AACACTGAGC ATGATCCTCC GTATTGGAGA 
GGTCCTATTT GGATAAACAT GAATTACAGA ATTCTTTCAG CGCTCCATCA TTACTCCAAA 
GAGAATGGAC CATATCAGGA CAAAGCCAAA GCTATTTACA CAGAATTGAG AAGCAATTTA 
ATTAGAAATA TAGTACGTAA TTATCAAGAG ACTGGATTTT TTTGGGAGCA GTACGATCAG 
GTTAAAGGCA AGGGAAAAGG AGCACGTCCA TTTACGGGTT GGACATCACT TGTAGTATTA 
ATCATGGCAG AAGCATATGG AACAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013446555.1
CDS	179..2728
Translation	MTGSGRTTARSRVRSSSEPDAGEGRPKFNRRRATSKYGGSIPLFDNVSIKIVLAISFIAFFIILFLIRHLVNSVAEPRLPRAVTPLPAPKIMDLDQFQGEHKESLYWGTYRPYLYLGIRARTPQSLMAGLMWIGVKDGRYHLRHVCRHEDDLSTYGWKKHNGRDYGHQVLVDHGMILTTEFLKSKGDDSGYGGDWAVRIDVQVDKSKWNEEFGRGAQLFFYLADEGGNVLDVSRENLDSRESSLLASGSRSDIGDWQLHLKSKDDLKLHYSGFHTPHFHNLSDLVEENLASQIRKHAQLQLSDSSDESSNVLAFQILGGFPFTTDIVLISGTDSGSSRVEERINSLTGASLSNELKYKEQAFDEKIEKVFNLTEKVDSESVSAGKAAIGSLLGGIGYFYGQSKIALSRTLNFREHVDYVSYWPAELYTAVPSRSFFPRGFLWDEGFHQLLIWRWDIRISLDIIGHWLDLMNVDGWIPREQILGAEALSRVPDEFVPQHPTNGNPPTMFLALSDIITGLKNNEFTAKDRSEISMFLERSFVRLEAWFQWFNTTQSGKQTSSYYWHGRDNRTMRELNPKSLSSGLDDYPRASHPSEDERHLDLRCWMLLAADCMHSIEKLLDKETRPGKNYGSTAKLLSDHELLNQMHFDDAYGAYFDFGNHTEKAQLKWKEMETGQNYASRQLVRDVSKRPVLRFVPHIGYVSLFPFMGRIIPPGSWILEKQLELISNNSLLWTDYGLRSLAKTSSMYMKRNTEHDPPYWRGPIWINMNYRILSALHHYSKENGPYQDKAKAIYTELRSNLIRNIVRNYQETGFFWEQYDQVKGKGKGARPFTGWTSLVVLIMAEAYGTI

Target ORF information:

RefSeq Version	XM_013591101.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula mannosyl-oligosaccharide glucosidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013591101.1

ORF Insert Sequence:

ATGACCGGAA GCGGAAGAAC AACCGCTCGG AGCAGAGTCA GATCTTCCTC TGAACCCGAC 
GCCGGCGAAG GAAGGCCGAA ATTTAACCGC CGCAGAGCCA CAAGCAAATA CGGAGGCAGC 
ATTCCGTTAT TCGACAATGT CAGCATCAAA ATTGTTCTCG CTATCAGCTT CATCGCATTC 
TTCATTATTT TGTTTCTCAT TCGTCACTTA GTTAATTCCG TTGCTGAACC TCGTCTTCCT 
CGCGCTGTTA CGCCGCTTCC CGCGCCAAAG ATCATGGACC TCGATCAGTT TCAAGGTGAG 
CACAAAGAGA GTTTGTATTG GGGAACATAT CGTCCTTATC TCTATCTCGG AATTCGTGCC 
AGGACTCCAC AATCTTTGAT GGCTGGATTA ATGTGGATTG GTGTGAAGGA CGGTAGATAT 
CACTTGAGGC ATGTTTGTAG ACACGAGGAT GACTTAAGTA CTTATGGTTG GAAAAAGCAT 
AATGGACGTG ATTATGGGCA CCAAGTACTA GTTGACCATG GCATGATATT GACTACCGAG 
TTTTTAAAAT CAAAGGGTGA TGACAGTGGT TATGGAGGGG ACTGGGCAGT TCGAATTGAT 
GTGCAAGTTG ATAAGTCTAA GTGGAATGAA GAATTTGGGA GAGGTGCCCA GCTCTTTTTC 
TATTTGGCCG ATGAAGGTGG TAATGTCCTA GATGTAAGTA GAGAAAACTT GGACAGTCGT 
GAGAGTTCTT TACTTGCATC TGGCTCTCGA TCAGACATTG GAGATTGGCA GCTACATTTA 
AAGTCCAAGG ATGATCTGAA ACTGCATTAT TCTGGATTTC ACACACCTCA CTTTCACAAT 
TTGTCTGATC TTGTTGAGGA AAATCTTGCA TCACAAATAA GAAAGCATGC TCAGCTGCAA 
CTATCTGACT CATCAGACGA ATCTTCTAAC GTGTTGGCTT TTCAGATTCT TGGTGGCTTT 
CCTTTCACTA CAGATATTGT GCTAATATCA GGAACTGATT CCGGAAGTTC AAGAGTGGAA 
GAGCGCATCA ATAGTCTTAC AGGAGCCTCA CTGTCCAATG AACTGAAATA TAAAGAACAA 
GCATTTGATG AAAAGATTGA GAAAGTTTTC AATTTGACAG AGAAGGTGGA CTCAGAATCC 
GTTTCTGCTG GTAAGGCTGC TATTGGAAGC TTATTAGGTG GCATCGGTTA CTTCTATGGC 
CAATCAAAAA TTGCTCTGTC AAGAACCCTT AATTTTAGGG AACATGTTGA TTATGTATCA 
TATTGGCCAG CTGAGCTTTA TACAGCTGTA CCAAGTCGGT CTTTCTTTCC AAGAGGATTT 
CTATGGGATG AAGGTTTTCA TCAACTTCTA ATCTGGCGTT GGGATATTCG TATTTCGTTG 
GATATCATCG GTCACTGGTT AGATTTGATG AACGTTGATG GATGGATACC ACGTGAACAA 
ATTCTGGGGG CTGAAGCATT AAGTAGGGTC CCAGATGAAT TTGTTCCTCA GCATCCAACA 
AATGGAAATC CTCCAACTAT GTTTTTAGCA TTAAGTGACA TAATTACTGG CTTGAAGAAT 
AATGAGTTCA CTGCAAAGGA TAGAAGTGAG ATCTCTATGT TCCTAGAACG CTCCTTTGTT 
CGATTAGAAG CATGGTTCCA ATGGTTCAAT ACAACTCAGT CAGGCAAACA AACGAGCAGC 
TACTACTGGC ATGGACGAGA TAACAGAACA ATGCGTGAAT TAAATCCCAA GTCACTGTCC 
TCTGGATTGG ATGATTACCC ACGTGCTTCA CACCCAAGTG AAGATGAACG CCACTTGGAT 
CTTAGATGCT GGATGTTACT AGCAGCAGAC TGTATGCATT CCATTGAGAA GTTGTTGGAT 
AAAGAAACCA GGCCTGGCAA GAATTATGGA TCTACTGCTA AATTGCTATC AGATCATGAG 
TTACTGAACC AGATGCATTT TGATGATGCT TATGGTGCTT ACTTTGATTT TGGAAATCAT 
ACAGAAAAGG CTCAACTAAA ATGGAAAGAG ATGGAGACTG GACAAAATTA TGCTTCTCGT 
CAGCTTGTTC GGGATGTTTC GAAGAGGCCT GTATTAAGAT TCGTTCCTCA CATTGGTTAT 
GTTAGTCTGT TCCCATTCAT GGGGAGAATC ATTCCACCTG GATCATGGAT TCTGGAAAAG 
CAGCTTGAAC TCATTTCAAA TAACAGCCTC TTATGGACTG ACTATGGACT ACGTTCTCTT 
GCCAAAACAA GTTCCATGTA CATGAAACGC AACACTGAGC ATGATCCTCC GTATTGGAGA 
GGTCCTATTT GGATAAACAT GAATTACAGA ATTCTTTCAG CGCTCCATCA TTACTCCAAA 
GAGAATGGAC CATATCAGGA CAAAGCCAAA GCTATTTACA CAGAATTGAG AAGCAATTTA 
ATTAGAAATA TAGTACGTAA TTATCAAGAG ACTGGATTTT TTTGGGAGCA GTACGATCAG 
GTTAAAGGCA AGGGAAAAGG AGCACGTCCA TTTACGGGTT GGACATCACT TGTAGTATTA 
ATCATGGCAG AAGCATATGG AACAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25501732 ( XM_013591101.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013446555.1 Medicago truncatula mannosyl-oligosaccharide glucosidase GCS1 (LOC25501732), mRNA.

Price & Availability↑

CloneID	OMe166452
Clone ID Related Accession (Same CDS sequence)	XM_013591101.1 , XM_013591101.2
Accession Version	XM_013591101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	mannosyl-oligosaccharide glucosidase GCS1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013591101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGGAA GCGGAAGAAC AACCGCTCGG AGCAGAGTCA GATCTTCCTC TGAACCCGAC 
GCCGGCGAAG GAAGGCCGAA ATTTAACCGC CGCAGAGCCA CAAGCAAATA CGGAGGCAGC 
ATTCCGTTAT TCGACAATGT CAGCATCAAA ATTGTTCTCG CTATCAGCTT CATCGCATTC 
TTCATTATTT TGTTTCTCAT TCGTCACTTA GTTAATTCCG TTGCTGAACC TCGTCTTCCT 
CGCGCTGTTA CGCCGCTTCC CGCGCCAAAG ATCATGGACC TCGATCAGTT TCAAGGTGAG 
CACAAAGAGA GTTTGTATTG GGGAACATAT CGTCCTTATC TCTATCTCGG AATTCGTGCC 
AGGACTCCAC AATCTTTGAT GGCTGGATTA ATGTGGATTG GTGTGAAGGA CGGTAGATAT 
CACTTGAGGC ATGTTTGTAG ACACGAGGAT GACTTAAGTA CTTATGGTTG GAAAAAGCAT 
AATGGACGTG ATTATGGGCA CCAAGTACTA GTTGACCATG GCATGATATT GACTACCGAG 
TTTTTAAAAT CAAAGGGTGA TGACAGTGGT TATGGAGGGG ACTGGGCAGT TCGAATTGAT 
GTGCAAGTTG ATAAGTCTAA GTGGAATGAA GAATTTGGGA GAGGTGCCCA GCTCTTTTTC 
TATTTGGCCG ATGAAGGTGG TAATGTCCTA GATGTAAGTA GAGAAAACTT GGACAGTCGT 
GAGAGTTCTT TACTTGCATC TGGCTCTCGA TCAGACATTG GAGATTGGCA GCTACATTTA 
AAGTCCAAGG ATGATCTGAA ACTGCATTAT TCTGGATTTC ACACACCTCA CTTTCACAAT 
TTGTCTGATC TTGTTGAGGA AAATCTTGCA TCACAAATAA GAAAGCATGC TCAGCTGCAA 
CTATCTGACT CATCAGACGA ATCTTCTAAC GTGTTGGCTT TTCAGATTCT TGGTGGCTTT 
CCTTTCACTA CAGATATTGT GCTAATATCA GGAACTGATT CCGGAAGTTC AAGAGTGGAA 
GAGCGCATCA ATAGTCTTAC AGGAGCCTCA CTGTCCAATG AACTGAAATA TAAAGAACAA 
GCATTTGATG AAAAGATTGA GAAAGTTTTC AATTTGACAG AGAAGGTGGA CTCAGAATCC 
GTTTCTGCTG GTAAGGCTGC TATTGGAAGC TTATTAGGTG GCATCGGTTA CTTCTATGGC 
CAATCAAAAA TTGCTCTGTC AAGAACCCTT AATTTTAGGG AACATGTTGA TTATGTATCA 
TATTGGCCAG CTGAGCTTTA TACAGCTGTA CCAAGTCGGT CTTTCTTTCC AAGAGGATTT 
CTATGGGATG AAGGTTTTCA TCAACTTCTA ATCTGGCGTT GGGATATTCG TATTTCGTTG 
GATATCATCG GTCACTGGTT AGATTTGATG AACGTTGATG GATGGATACC ACGTGAACAA 
ATTCTGGGGG CTGAAGCATT AAGTAGGGTC CCAGATGAAT TTGTTCCTCA GCATCCAACA 
AATGGAAATC CTCCAACTAT GTTTTTAGCA TTAAGTGACA TAATTACTGG CTTGAAGAAT 
AATGAGTTCA CTGCAAAGGA TAGAAGTGAG ATCTCTATGT TCCTAGAACG CTCCTTTGTT 
CGATTAGAAG CATGGTTCCA ATGGTTCAAT ACAACTCAGT CAGGCAAACA AACGAGCAGC 
TACTACTGGC ATGGACGAGA TAACAGAACA ATGCGTGAAT TAAATCCCAA GTCACTGTCC 
TCTGGATTGG ATGATTACCC ACGTGCTTCA CACCCAAGTG AAGATGAACG CCACTTGGAT 
CTTAGATGCT GGATGTTACT AGCAGCAGAC TGTATGCATT CCATTGAGAA GTTGTTGGAT 
AAAGAAACCA GGCCTGGCAA GAATTATGGA TCTACTGCTA AATTGCTATC AGATCATGAG 
TTACTGAACC AGATGCATTT TGATGATGCT TATGGTGCTT ACTTTGATTT TGGAAATCAT 
ACAGAAAAGG CTCAACTAAA ATGGAAAGAG ATGGAGACTG GACAAAATTA TGCTTCTCGT 
CAGCTTGTTC GGGATGTTTC GAAGAGGCCT GTATTAAGAT TCGTTCCTCA CATTGGTTAT 
GTTAGTCTGT TCCCATTCAT GGGGAGAATC ATTCCACCTG GATCATGGAT TCTGGAAAAG 
CAGCTTGAAC TCATTTCAAA TAACAGCCTC TTATGGACTG ACTATGGACT ACGTTCTCTT 
GCCAAAACAA GTTCCATGTA CATGAAACGC AACACTGAGC ATGATCCTCC GTATTGGAGA 
GGTCCTATTT GGATAAACAT GAATTACAGA ATTCTTTCAG CGCTCCATCA TTACTCCAAA 
GAGAATGGAC CATATCAGGA CAAAGCCAAA GCTATTTACA CAGAATTGAG AAGCAATTTA 
ATTAGAAATA TAGTACGTAA TTATCAAGAG ACTGGATTTT TTTGGGAGCA GTACGATCAG 
GTTAAAGGCA AGGGAAAAGG AGCACGTCCA TTTACGGGTT GGACATCACT TGTAGTATTA 
ATCATGGCAG AAGCATATGG AACAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013446555.1
CDS	144..2693
Translation	MTGSGRTTARSRVRSSSEPDAGEGRPKFNRRRATSKYGGSIPLFDNVSIKIVLAISFIAFFIILFLIRHLVNSVAEPRLPRAVTPLPAPKIMDLDQFQGEHKESLYWGTYRPYLYLGIRARTPQSLMAGLMWIGVKDGRYHLRHVCRHEDDLSTYGWKKHNGRDYGHQVLVDHGMILTTEFLKSKGDDSGYGGDWAVRIDVQVDKSKWNEEFGRGAQLFFYLADEGGNVLDVSRENLDSRESSLLASGSRSDIGDWQLHLKSKDDLKLHYSGFHTPHFHNLSDLVEENLASQIRKHAQLQLSDSSDESSNVLAFQILGGFPFTTDIVLISGTDSGSSRVEERINSLTGASLSNELKYKEQAFDEKIEKVFNLTEKVDSESVSAGKAAIGSLLGGIGYFYGQSKIALSRTLNFREHVDYVSYWPAELYTAVPSRSFFPRGFLWDEGFHQLLIWRWDIRISLDIIGHWLDLMNVDGWIPREQILGAEALSRVPDEFVPQHPTNGNPPTMFLALSDIITGLKNNEFTAKDRSEISMFLERSFVRLEAWFQWFNTTQSGKQTSSYYWHGRDNRTMRELNPKSLSSGLDDYPRASHPSEDERHLDLRCWMLLAADCMHSIEKLLDKETRPGKNYGSTAKLLSDHELLNQMHFDDAYGAYFDFGNHTEKAQLKWKEMETGQNYASRQLVRDVSKRPVLRFVPHIGYVSLFPFMGRIIPPGSWILEKQLELISNNSLLWTDYGLRSLAKTSSMYMKRNTEHDPPYWRGPIWINMNYRILSALHHYSKENGPYQDKAKAIYTELRSNLIRNIVRNYQETGFFWEQYDQVKGKGKGARPFTGWTSLVVLIMAEAYGTI

Target ORF information:

RefSeq Version	XM_013591101.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula mannosyl-oligosaccharide glucosidase GCS1 (LOC25501732), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013591101.2

ORF Insert Sequence:

ATGACCGGAA GCGGAAGAAC AACCGCTCGG AGCAGAGTCA GATCTTCCTC TGAACCCGAC 
GCCGGCGAAG GAAGGCCGAA ATTTAACCGC CGCAGAGCCA CAAGCAAATA CGGAGGCAGC 
ATTCCGTTAT TCGACAATGT CAGCATCAAA ATTGTTCTCG CTATCAGCTT CATCGCATTC 
TTCATTATTT TGTTTCTCAT TCGTCACTTA GTTAATTCCG TTGCTGAACC TCGTCTTCCT 
CGCGCTGTTA CGCCGCTTCC CGCGCCAAAG ATCATGGACC TCGATCAGTT TCAAGGTGAG 
CACAAAGAGA GTTTGTATTG GGGAACATAT CGTCCTTATC TCTATCTCGG AATTCGTGCC 
AGGACTCCAC AATCTTTGAT GGCTGGATTA ATGTGGATTG GTGTGAAGGA CGGTAGATAT 
CACTTGAGGC ATGTTTGTAG ACACGAGGAT GACTTAAGTA CTTATGGTTG GAAAAAGCAT 
AATGGACGTG ATTATGGGCA CCAAGTACTA GTTGACCATG GCATGATATT GACTACCGAG 
TTTTTAAAAT CAAAGGGTGA TGACAGTGGT TATGGAGGGG ACTGGGCAGT TCGAATTGAT 
GTGCAAGTTG ATAAGTCTAA GTGGAATGAA GAATTTGGGA GAGGTGCCCA GCTCTTTTTC 
TATTTGGCCG ATGAAGGTGG TAATGTCCTA GATGTAAGTA GAGAAAACTT GGACAGTCGT 
GAGAGTTCTT TACTTGCATC TGGCTCTCGA TCAGACATTG GAGATTGGCA GCTACATTTA 
AAGTCCAAGG ATGATCTGAA ACTGCATTAT TCTGGATTTC ACACACCTCA CTTTCACAAT 
TTGTCTGATC TTGTTGAGGA AAATCTTGCA TCACAAATAA GAAAGCATGC TCAGCTGCAA 
CTATCTGACT CATCAGACGA ATCTTCTAAC GTGTTGGCTT TTCAGATTCT TGGTGGCTTT 
CCTTTCACTA CAGATATTGT GCTAATATCA GGAACTGATT CCGGAAGTTC AAGAGTGGAA 
GAGCGCATCA ATAGTCTTAC AGGAGCCTCA CTGTCCAATG AACTGAAATA TAAAGAACAA 
GCATTTGATG AAAAGATTGA GAAAGTTTTC AATTTGACAG AGAAGGTGGA CTCAGAATCC 
GTTTCTGCTG GTAAGGCTGC TATTGGAAGC TTATTAGGTG GCATCGGTTA CTTCTATGGC 
CAATCAAAAA TTGCTCTGTC AAGAACCCTT AATTTTAGGG AACATGTTGA TTATGTATCA 
TATTGGCCAG CTGAGCTTTA TACAGCTGTA CCAAGTCGGT CTTTCTTTCC AAGAGGATTT 
CTATGGGATG AAGGTTTTCA TCAACTTCTA ATCTGGCGTT GGGATATTCG TATTTCGTTG 
GATATCATCG GTCACTGGTT AGATTTGATG AACGTTGATG GATGGATACC ACGTGAACAA 
ATTCTGGGGG CTGAAGCATT AAGTAGGGTC CCAGATGAAT TTGTTCCTCA GCATCCAACA 
AATGGAAATC CTCCAACTAT GTTTTTAGCA TTAAGTGACA TAATTACTGG CTTGAAGAAT 
AATGAGTTCA CTGCAAAGGA TAGAAGTGAG ATCTCTATGT TCCTAGAACG CTCCTTTGTT 
CGATTAGAAG CATGGTTCCA ATGGTTCAAT ACAACTCAGT CAGGCAAACA AACGAGCAGC 
TACTACTGGC ATGGACGAGA TAACAGAACA ATGCGTGAAT TAAATCCCAA GTCACTGTCC 
TCTGGATTGG ATGATTACCC ACGTGCTTCA CACCCAAGTG AAGATGAACG CCACTTGGAT 
CTTAGATGCT GGATGTTACT AGCAGCAGAC TGTATGCATT CCATTGAGAA GTTGTTGGAT 
AAAGAAACCA GGCCTGGCAA GAATTATGGA TCTACTGCTA AATTGCTATC AGATCATGAG 
TTACTGAACC AGATGCATTT TGATGATGCT TATGGTGCTT ACTTTGATTT TGGAAATCAT 
ACAGAAAAGG CTCAACTAAA ATGGAAAGAG ATGGAGACTG GACAAAATTA TGCTTCTCGT 
CAGCTTGTTC GGGATGTTTC GAAGAGGCCT GTATTAAGAT TCGTTCCTCA CATTGGTTAT 
GTTAGTCTGT TCCCATTCAT GGGGAGAATC ATTCCACCTG GATCATGGAT TCTGGAAAAG 
CAGCTTGAAC TCATTTCAAA TAACAGCCTC TTATGGACTG ACTATGGACT ACGTTCTCTT 
GCCAAAACAA GTTCCATGTA CATGAAACGC AACACTGAGC ATGATCCTCC GTATTGGAGA 
GGTCCTATTT GGATAAACAT GAATTACAGA ATTCTTTCAG CGCTCCATCA TTACTCCAAA 
GAGAATGGAC CATATCAGGA CAAAGCCAAA GCTATTTACA CAGAATTGAG AAGCAATTTA 
ATTAGAAATA TAGTACGTAA TTATCAAGAG ACTGGATTTT TTTGGGAGCA GTACGATCAG 
GTTAAAGGCA AGGGAAAAGG AGCACGTCCA TTTACGGGTT GGACATCACT TGTAGTATTA 
ATCATGGCAG AAGCATATGG AACAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25501732 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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