LOC25495021 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25495021
Entrez Gene ID	25495021
Full Name	phosphatidylinositol-4-phosphate 5-kinase family protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013598540.1	XP_013453994.1	phosphatidylinositol-4-phosphate 5-kinase family protein
XM_024784090.1	XP_024639858.1	phosphatidylinositol 4-phosphate 5-kinase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25495021 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25495021 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe182201	XM_013598540.1 Latest version!	Medicago truncatula phosphatidylinositol-4-phosphate 5-kinase family protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe265176	XM_024784090.1 Latest version!	Medicago truncatula phosphatidylinositol 4-phosphate 5-kinase 1 (LOC25495021), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25495021 ( XM_013598540.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013453994.1 Medicago truncatula phosphatidylinositol-4-phosphate 5-kinase family protein partial mRNA.

Price & Availability↑

CloneID	OMe182201
Clone ID Related Accession (Same CDS sequence)	XM_013598540.1
Accession Version	XM_013598540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	phosphatidylinositol-4-phosphate 5-kinase family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016411). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGATA CAGTGGTGAA CGATGAAGTT GTTGAGGTTG TTAGTTCAAC GAAGAACAAC 
ATTCCGGAGC CGGAGAAGGA AAGCAAACCT TCTCCACCGG CGGTGATTGC TGGAAGAAGC 
CGTTCACAAG GAGGAGGTAG AAGAGTGACA CCGACGAGCT TCACGGTGGT TTCTGATGGT 
AGATCCGTTG TTGAGAGAGT ACTTCCGAAC GGAGATTTCT ATGCCGGAAG TTTCTCCGGT 
AACGTACCGA ATGGATCCGG GAAGTATCTA TGGACCGACG GTTGCATGTA CGAAGGTGAA 
TGGAAACGAG GTAAAGCTTC AGGGAAAGGG AAATTTTCAT GGCCTTCAGG CGCGACTTAC 
GAGGGAGAGT TCAAGTCGGG TCGAATGGAA GGATTCGGAA CATTCGTTGG ATCCGACGGT 
GATACATATC GTGGGTCATG GAGCTCCGAT AGAAAACACG GGTTCGGTCA GAAACGTTAC 
GCGAACGGTG ATTTATACGA AGGGTGGTGG AAGCGTAACG TTCAAGATGG TCAAGGACGT 
TACGTTTGGA AGAATGGGAA TGAGTATGTT GGTGAATGGC GTAACGGCGT TATATCTGGG 
AAGGGTTCGT TAGTTTGGGT TAACGGAAAC CGTTTGGAGG GTTTGTGGGA GAATGGTGTT 
CCGAAAGGAC AAGGTGTTAT GAAGATTCAA CATGGTTTTG GTGGGTTTGT TTGGAATGAC 
AATTTTGCCC CTGTGATGAT GAGGAAACGT TCTTCTGTTG ATACTACTGT TAGTGGTAGA 
GGGAGTTTGA ATGAAAAGAA TTTTCCTAGG ATTTGTATTT GGGAATCTGA AGGTGAAGCT 
GGTGATATTA CTTGTGATAT TGTTGATAAT GTTGAAGCTT CCATGTTTTA CCGCGATGGG 
ACGTGTTCCG ATCCTGATGA GTTTCGAAAA CATCCTTGTT GTTTCTCTTC TGAGGTTAAG 
AGACCTGGTC AAACTATTTC TAAAGGGCAT AAGAATTATG ATTTGATGCT TAATTTGCAA 
TTGGGGATTA GATACACGGT TGGGAAGGAA GCATCGAAGT TGCGAGAGCT TAAACCTAGT 
GATTTTGATC CTAAGGAGAA GTTTTGGACA AGGTTTCCGA GTGAAGGATC CAAGATTACC 
CCGCCTCATC AAACCGCGGA GTTTCGATGG AAAGATTACT GTCCTGTTGT TTTTAGACAT 
TTGAGGAAGC TGTTTCAGGT TGATCCTGCT GATTATATGT TGGCTATATG TGGGGATGAT 
GCTCTCAGAG AACTTTCCTC TCCCGGGAAA AGCGGAAGTG TTTTCTACTT GACCCAAGAT 
GACAGATTCA TGATTAAGAC AGTGAAGAAA TCTGAAGTCA AGGTGCTTCT TCGGATGCTT 
CGAAGCTATT ACAAACACGT GTCCAAATAT GAGAATTCAC TGGTGACAAA ATTCTTTGGG 
GTACATTGCG TGAAACCAAT TGGAGGCCAA AAGATCCGGT TTATTGTGAT GGGCAATCTT 
TTCTGCTCTG AATATCCAAT TCATAGACGA TTTGATTTGA AAGGATCTTC ACATGGTCGT 
GCAACAGATA AACCTGAGGA TGAGATTGAT GAAACAACCA CCCTCAAAGA CCTTGATCTG 
AACTATGTGT TTCGTGTACA GAGAAATTGG TACCAGGATC TTATTAAACA AATTGAGCGG 
GATTGTGAGT TCTTGGAAAA CGAGAAAATT ATGGACTACA GTCTTTTGGT TGGCCTTCAT 
TTTCGTGATG ACAACACTTA CGACAAAATG GGGTTGTCAC CTTTTCTTCT ACGCACTGGC 
ACTCAGGATT CATATCATAC TGAGAAGTTC ATGCGTGGTT ACCGCTTTCT CGAAGCAGAG 
CTGCAAGATA GGGATCGAGT TAAATCTGGA AGGAAATCAT TAATCAGGCT AGGAGCCAAT 
ATGCCAGCAA GAGCGGAACG ATTGGCAAGG AGGAGTGATT TTGATCAATA CACCAATGGT 
GGAATCAGTC ATTTGAATCC TTATAGCAGT GGGGAAGCTT ATGATTGCGT TCTATATTTT 
GGGGTTATCG ATATTTTGCA AGACTATGAT ATCAGTAAGA AGCTTGAGCA TGCTTACAAG 
TCTTTACAAG TTGACTCTAC TTCAATTTCT GCTGTTGATC CAAAGCTATA CTCAAAAAGG 
TTTCGAGATT TTATTGGAAG AATATTCATT GAAGATTGGA AAAATATTTG GAAGTTTGAA 
ACCCACTTGC AAAGGAGGGA CCTTAGAGTT GACTGTTCAT CAGATCACTT GAAAGTGAGT 
GTGTTGGACT TGAAAAAGAG TTTTATTAGA CGACATAAAA AGCAGAGAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013453994.1
CDS	1..2334
Translation	MSDTVVNDEVVEVVSSTKNNIPEPEKESKPSPPAVIAGRSRSQGGGRRVTPTSFTVVSDGRSVVERVLPNGDFYAGSFSGNVPNGSGKYLWTDGCMYEGEWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGTFVGSDGDTYRGSWSSDRKHGFGQKRYANGDLYEGWWKRNVQDGQGRYVWKNGNEYVGEWRNGVISGKGSLVWVNGNRLEGLWENGVPKGQGVMKIQHGFGGFVWNDNFAPVMMRKRSSVDTTVSGRGSLNEKNFPRICIWESEGEAGDITCDIVDNVEASMFYRDGTCSDPDEFRKHPCCFSSEVKRPGQTISKGHKNYDLMLNLQLGIRYTVGKEASKLRELKPSDFDPKEKFWTRFPSEGSKITPPHQTAEFRWKDYCPVVFRHLRKLFQVDPADYMLAICGDDALRELSSPGKSGSVFYLTQDDRFMIKTVKKSEVKVLLRMLRSYYKHVSKYENSLVTKFFGVHCVKPIGGQKIRFIVMGNLFCSEYPIHRRFDLKGSSHGRATDKPEDEIDETTTLKDLDLNYVFRVQRNWYQDLIKQIERDCEFLENEKIMDYSLLVGLHFRDDNTYDKMGLSPFLLRTGTQDSYHTEKFMRGYRFLEAELQDRDRVKSGRKSLIRLGANMPARAERLARRSDFDQYTNGGISHLNPYSSGEAYDCVLYFGVIDILQDYDISKKLEHAYKSLQVDSTSISAVDPKLYSKRFRDFIGRIFIEDWKNIWKFETHLQRRDLRVDCSSDHLKVSVLDLKKSFIRRHKKQRK

Target ORF information:

RefSeq Version	XM_013598540.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula phosphatidylinositol-4-phosphate 5-kinase family protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013598540.1

ORF Insert Sequence:

ATGAGTGATA CAGTGGTGAA CGATGAAGTT GTTGAGGTTG TTAGTTCAAC GAAGAACAAC 
ATTCCGGAGC CGGAGAAGGA AAGCAAACCT TCTCCACCGG CGGTGATTGC TGGAAGAAGC 
CGTTCACAAG GAGGAGGTAG AAGAGTGACA CCGACGAGCT TCACGGTGGT TTCTGATGGT 
AGATCCGTTG TTGAGAGAGT ACTTCCGAAC GGAGATTTCT ATGCCGGAAG TTTCTCCGGT 
AACGTACCGA ATGGATCCGG GAAGTATCTA TGGACCGACG GTTGCATGTA CGAAGGTGAA 
TGGAAACGAG GTAAAGCTTC AGGGAAAGGG AAATTTTCAT GGCCTTCAGG CGCGACTTAC 
GAGGGAGAGT TCAAGTCGGG TCGAATGGAA GGATTCGGAA CATTCGTTGG ATCCGACGGT 
GATACATATC GTGGGTCATG GAGCTCCGAT AGAAAACACG GGTTCGGTCA GAAACGTTAC 
GCGAACGGTG ATTTATACGA AGGGTGGTGG AAGCGTAACG TTCAAGATGG TCAAGGACGT 
TACGTTTGGA AGAATGGGAA TGAGTATGTT GGTGAATGGC GTAACGGCGT TATATCTGGG 
AAGGGTTCGT TAGTTTGGGT TAACGGAAAC CGTTTGGAGG GTTTGTGGGA GAATGGTGTT 
CCGAAAGGAC AAGGTGTTAT GAAGATTCAA CATGGTTTTG GTGGGTTTGT TTGGAATGAC 
AATTTTGCCC CTGTGATGAT GAGGAAACGT TCTTCTGTTG ATACTACTGT TAGTGGTAGA 
GGGAGTTTGA ATGAAAAGAA TTTTCCTAGG ATTTGTATTT GGGAATCTGA AGGTGAAGCT 
GGTGATATTA CTTGTGATAT TGTTGATAAT GTTGAAGCTT CCATGTTTTA CCGCGATGGG 
ACGTGTTCCG ATCCTGATGA GTTTCGAAAA CATCCTTGTT GTTTCTCTTC TGAGGTTAAG 
AGACCTGGTC AAACTATTTC TAAAGGGCAT AAGAATTATG ATTTGATGCT TAATTTGCAA 
TTGGGGATTA GATACACGGT TGGGAAGGAA GCATCGAAGT TGCGAGAGCT TAAACCTAGT 
GATTTTGATC CTAAGGAGAA GTTTTGGACA AGGTTTCCGA GTGAAGGATC CAAGATTACC 
CCGCCTCATC AAACCGCGGA GTTTCGATGG AAAGATTACT GTCCTGTTGT TTTTAGACAT 
TTGAGGAAGC TGTTTCAGGT TGATCCTGCT GATTATATGT TGGCTATATG TGGGGATGAT 
GCTCTCAGAG AACTTTCCTC TCCCGGGAAA AGCGGAAGTG TTTTCTACTT GACCCAAGAT 
GACAGATTCA TGATTAAGAC AGTGAAGAAA TCTGAAGTCA AGGTGCTTCT TCGGATGCTT 
CGAAGCTATT ACAAACACGT GTCCAAATAT GAGAATTCAC TGGTGACAAA ATTCTTTGGG 
GTACATTGCG TGAAACCAAT TGGAGGCCAA AAGATCCGGT TTATTGTGAT GGGCAATCTT 
TTCTGCTCTG AATATCCAAT TCATAGACGA TTTGATTTGA AAGGATCTTC ACATGGTCGT 
GCAACAGATA AACCTGAGGA TGAGATTGAT GAAACAACCA CCCTCAAAGA CCTTGATCTG 
AACTATGTGT TTCGTGTACA GAGAAATTGG TACCAGGATC TTATTAAACA AATTGAGCGG 
GATTGTGAGT TCTTGGAAAA CGAGAAAATT ATGGACTACA GTCTTTTGGT TGGCCTTCAT 
TTTCGTGATG ACAACACTTA CGACAAAATG GGGTTGTCAC CTTTTCTTCT ACGCACTGGC 
ACTCAGGATT CATATCATAC TGAGAAGTTC ATGCGTGGTT ACCGCTTTCT CGAAGCAGAG 
CTGCAAGATA GGGATCGAGT TAAATCTGGA AGGAAATCAT TAATCAGGCT AGGAGCCAAT 
ATGCCAGCAA GAGCGGAACG ATTGGCAAGG AGGAGTGATT TTGATCAATA CACCAATGGT 
GGAATCAGTC ATTTGAATCC TTATAGCAGT GGGGAAGCTT ATGATTGCGT TCTATATTTT 
GGGGTTATCG ATATTTTGCA AGACTATGAT ATCAGTAAGA AGCTTGAGCA TGCTTACAAG 
TCTTTACAAG TTGACTCTAC TTCAATTTCT GCTGTTGATC CAAAGCTATA CTCAAAAAGG 
TTTCGAGATT TTATTGGAAG AATATTCATT GAAGATTGGA AAAATATTTG GAAGTTTGAA 
ACCCACTTGC AAAGGAGGGA CCTTAGAGTT GACTGTTCAT CAGATCACTT GAAAGTGAGT 
GTGTTGGACT TGAAAAAGAG TTTTATTAGA CGACATAAAA AGCAGAGAAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25495021 ( XM_024784090.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024639858.1 Medicago truncatula phosphatidylinositol 4-phosphate 5-kinase 1 (LOC25495021), mRNA.

Price & Availability↑

CloneID	OMe265176
Clone ID Related Accession (Same CDS sequence)	XM_024784090.1
Accession Version	XM_024784090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	phosphatidylinositol 4-phosphate 5-kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016411.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGATA CAGTGGTGAA CGATGAAGTT GTTGAGGTTG TTAGTTCAAC GAAGAACAAC 
ATTCCGGAGC CGGAGAAGGA AAGCAAACCT TCTCCACCGG CGGTGATTGC TGGAAGAAGC 
CGTTCACAAG GAGGAGGTAG AAGAGTGACA CCGACGAGCT TCACGGTGGT TTCTGATGGT 
AGATCCGTTG TTGAGAGAGT ACTTCCGAAC GGAGATTTCT ATGCCGGAAG TTTCTCCGGT 
AACGTACCGA ATGGATCCGG GAAGTATCTA TGGACCGACG GTTGCATGTA CGAAGGTGAA 
TGGAAACGAG GTAAAGCTTC AGGGAAAGGG AAATTTTCAT GGCCTTCAGG CGCGACTTAC 
GAGGGAGAGT TCAAGTCGGG TCGAATGGAA GGATTCGGAA CATTCGTTGG ATCCGACGGT 
GATACATATC GTGGGTCATG GAGCTCCGAT AGAAAACACG GGTTCGGTCA GAAACGTTAC 
GCGAACGGTG ATTTATACGA AGGGTGGTGG AAGCGTAACG TTCAAGATGG TCAAGGACGT 
TACGTTTGGA AGAATGGGAA TGAGTATGTT GGTGAATGGC GTAACGGCGT TATATCTGGG 
AAGGGTTCGT TAGTTTGGGT TAACGGAAAC CGTTTGGAGG GTTTGTGGGA GAATGGTGTT 
CCGAAAGGAC AAGGTGTTAT GAAGATTCAA CATGGTTTTG GTGGGTTTGT TTGGAATGAC 
AATTTTGCCC CTGTGATGAT GAGGAAACGT TCTTCTGTTG ATACTACTGT TAGTGGTAGA 
GGGAGTTTGA ATGAAAAGAA TTTTCCTAGG ATTTGTATTT GGGAATCTGA AGGTGAAGCT 
GGTGATATTA CTTGTGATAT TGTTGATAAT GTTGAAGCTT CCATGTTTTA CCGCGATGGG 
ACGTGTTCCG ATCCTGATGA GTTTCGAAAA CATCCTTGTT GTTTCTCTTC TGAGGTTAAG 
AGACCTGGTC AAACTATTTC TAAAGGGCAT AAGAATTATG ATTTGATGCT TAATTTGCAA 
TTGGGGATTA GATACACGGT TGGGAAGGAA GCATCGAAGT TGCGAGAGCT TAAACCTAGT 
GATTTTGATC CTAAGGAGAA GTTTTGGACA AGGTTTCCGA GTGAAGGATC CAAGATTACC 
CCGCCTCATC AAACCGCGGA GTTTCGATGG AAAGATTACT GTCCTGTTGT TTTTAGACAT 
TTGAGGAAGC TGTTTCAGGT TGATCCTGCT GATTATATGT TGGCTATATG TGGGGATGAT 
GCTCTCAGAG AACTTTCCTC TCCCGGGAAA AGCGGAAGTG TTTTCTACTT GACCCAAGAT 
GACAGATTCA TGATTAAGAC AGTGAAGAAA TCTGAAGTCA AGGTGCTTCT TCGGATGCTT 
CGAAGCTATT ACAAACACGT GTCCAAATAT GAGAATTCAC TGGTGACAAA ATTCTTTGGG 
GTACATTGCG TGAAACCAAT TGGAGGCCAA AAGATCCGGT TTATTGTGAT GGGCAATCTT 
TTCTGCTCTG AATATCCAAT TCATAGACGA TTTGATTTGA AAGGATCTTC ACATGGTCGT 
GCAACAGATA AACCTGAGGA TGAGATTGAT GAAACAACCA CCCTCAAAGA CCTTGATCTG 
AACTATGTGT TTCGTGTACA GAGAAATTGG TACCAGGATC TTATTAAACA AATTGAGCGG 
GATTGTGAGT TCTTGGAAAA CGAGAAAATT ATGGACTACA GTCTTTTGGT TGGCCTTCAT 
TTTCGTGATG ACAACACTTA CGACAAAATG GGGTTGTCAC CTTTTCTTCT ACGCACTGGC 
ACTCAGGATT CATATCATAC TGAGAAGTTC ATGCGTGGTT ACCGCTTTCT CGAAGCAGAG 
CTGCAAGATA GGGATCGAGT TAAATCTGGA AGGAAATCAT TAATCAGGCT AGGAGCCAAT 
ATGCCAGCAA GAGCGGAACG ATTGGCAAGG AGGAGTGATT TTGATCAATA CACCAATGGT 
GGAATCAGTC ATTTGAATCC TTATAGCAGT GGGGAAGCTT ATGATTGCGT TCTATATTTT 
GGGGTTATCG ATATTTTGCA AGACTATGAT ATCAGTAAGA AGCTTGAGCA TGCTTACAAG 
TCTTTACAAG TTGACTCTAC TTCAATTTCT GCTGTTGATC CAAAGCTATA CTCAAAAAGG 
TTTCGAGATT TTATTGGAAG AATATTCATT GAAGATTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024639858.1
CDS	202..2403
Translation	MSDTVVNDEVVEVVSSTKNNIPEPEKESKPSPPAVIAGRSRSQGGGRRVTPTSFTVVSDGRSVVERVLPNGDFYAGSFSGNVPNGSGKYLWTDGCMYEGEWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGTFVGSDGDTYRGSWSSDRKHGFGQKRYANGDLYEGWWKRNVQDGQGRYVWKNGNEYVGEWRNGVISGKGSLVWVNGNRLEGLWENGVPKGQGVMKIQHGFGGFVWNDNFAPVMMRKRSSVDTTVSGRGSLNEKNFPRICIWESEGEAGDITCDIVDNVEASMFYRDGTCSDPDEFRKHPCCFSSEVKRPGQTISKGHKNYDLMLNLQLGIRYTVGKEASKLRELKPSDFDPKEKFWTRFPSEGSKITPPHQTAEFRWKDYCPVVFRHLRKLFQVDPADYMLAICGDDALRELSSPGKSGSVFYLTQDDRFMIKTVKKSEVKVLLRMLRSYYKHVSKYENSLVTKFFGVHCVKPIGGQKIRFIVMGNLFCSEYPIHRRFDLKGSSHGRATDKPEDEIDETTTLKDLDLNYVFRVQRNWYQDLIKQIERDCEFLENEKIMDYSLLVGLHFRDDNTYDKMGLSPFLLRTGTQDSYHTEKFMRGYRFLEAELQDRDRVKSGRKSLIRLGANMPARAERLARRSDFDQYTNGGISHLNPYSSGEAYDCVLYFGVIDILQDYDISKKLEHAYKSLQVDSTSISAVDPKLYSKRFRDFIGRIFIEDW

Target ORF information:

RefSeq Version	XM_024784090.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula phosphatidylinositol 4-phosphate 5-kinase 1 (LOC25495021), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024784090.1

ORF Insert Sequence:

ATGAGTGATA CAGTGGTGAA CGATGAAGTT GTTGAGGTTG TTAGTTCAAC GAAGAACAAC 
ATTCCGGAGC CGGAGAAGGA AAGCAAACCT TCTCCACCGG CGGTGATTGC TGGAAGAAGC 
CGTTCACAAG GAGGAGGTAG AAGAGTGACA CCGACGAGCT TCACGGTGGT TTCTGATGGT 
AGATCCGTTG TTGAGAGAGT ACTTCCGAAC GGAGATTTCT ATGCCGGAAG TTTCTCCGGT 
AACGTACCGA ATGGATCCGG GAAGTATCTA TGGACCGACG GTTGCATGTA CGAAGGTGAA 
TGGAAACGAG GTAAAGCTTC AGGGAAAGGG AAATTTTCAT GGCCTTCAGG CGCGACTTAC 
GAGGGAGAGT TCAAGTCGGG TCGAATGGAA GGATTCGGAA CATTCGTTGG ATCCGACGGT 
GATACATATC GTGGGTCATG GAGCTCCGAT AGAAAACACG GGTTCGGTCA GAAACGTTAC 
GCGAACGGTG ATTTATACGA AGGGTGGTGG AAGCGTAACG TTCAAGATGG TCAAGGACGT 
TACGTTTGGA AGAATGGGAA TGAGTATGTT GGTGAATGGC GTAACGGCGT TATATCTGGG 
AAGGGTTCGT TAGTTTGGGT TAACGGAAAC CGTTTGGAGG GTTTGTGGGA GAATGGTGTT 
CCGAAAGGAC AAGGTGTTAT GAAGATTCAA CATGGTTTTG GTGGGTTTGT TTGGAATGAC 
AATTTTGCCC CTGTGATGAT GAGGAAACGT TCTTCTGTTG ATACTACTGT TAGTGGTAGA 
GGGAGTTTGA ATGAAAAGAA TTTTCCTAGG ATTTGTATTT GGGAATCTGA AGGTGAAGCT 
GGTGATATTA CTTGTGATAT TGTTGATAAT GTTGAAGCTT CCATGTTTTA CCGCGATGGG 
ACGTGTTCCG ATCCTGATGA GTTTCGAAAA CATCCTTGTT GTTTCTCTTC TGAGGTTAAG 
AGACCTGGTC AAACTATTTC TAAAGGGCAT AAGAATTATG ATTTGATGCT TAATTTGCAA 
TTGGGGATTA GATACACGGT TGGGAAGGAA GCATCGAAGT TGCGAGAGCT TAAACCTAGT 
GATTTTGATC CTAAGGAGAA GTTTTGGACA AGGTTTCCGA GTGAAGGATC CAAGATTACC 
CCGCCTCATC AAACCGCGGA GTTTCGATGG AAAGATTACT GTCCTGTTGT TTTTAGACAT 
TTGAGGAAGC TGTTTCAGGT TGATCCTGCT GATTATATGT TGGCTATATG TGGGGATGAT 
GCTCTCAGAG AACTTTCCTC TCCCGGGAAA AGCGGAAGTG TTTTCTACTT GACCCAAGAT 
GACAGATTCA TGATTAAGAC AGTGAAGAAA TCTGAAGTCA AGGTGCTTCT TCGGATGCTT 
CGAAGCTATT ACAAACACGT GTCCAAATAT GAGAATTCAC TGGTGACAAA ATTCTTTGGG 
GTACATTGCG TGAAACCAAT TGGAGGCCAA AAGATCCGGT TTATTGTGAT GGGCAATCTT 
TTCTGCTCTG AATATCCAAT TCATAGACGA TTTGATTTGA AAGGATCTTC ACATGGTCGT 
GCAACAGATA AACCTGAGGA TGAGATTGAT GAAACAACCA CCCTCAAAGA CCTTGATCTG 
AACTATGTGT TTCGTGTACA GAGAAATTGG TACCAGGATC TTATTAAACA AATTGAGCGG 
GATTGTGAGT TCTTGGAAAA CGAGAAAATT ATGGACTACA GTCTTTTGGT TGGCCTTCAT 
TTTCGTGATG ACAACACTTA CGACAAAATG GGGTTGTCAC CTTTTCTTCT ACGCACTGGC 
ACTCAGGATT CATATCATAC TGAGAAGTTC ATGCGTGGTT ACCGCTTTCT CGAAGCAGAG 
CTGCAAGATA GGGATCGAGT TAAATCTGGA AGGAAATCAT TAATCAGGCT AGGAGCCAAT 
ATGCCAGCAA GAGCGGAACG ATTGGCAAGG AGGAGTGATT TTGATCAATA CACCAATGGT 
GGAATCAGTC ATTTGAATCC TTATAGCAGT GGGGAAGCTT ATGATTGCGT TCTATATTTT 
GGGGTTATCG ATATTTTGCA AGACTATGAT ATCAGTAAGA AGCTTGAGCA TGCTTACAAG 
TCTTTACAAG TTGACTCTAC TTCAATTTCT GCTGTTGATC CAAAGCTATA CTCAAAAAGG 
TTTCGAGATT TTATTGGAAG AATATTCATT GAAGATTGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25495021 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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