MTR_7g090470 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_7g090470
Entrez Gene ID	11414821
Full Name	triacylglycerol lipase SDP1
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003625008.3	XP_003625056.1	triacylglycerol lipase SDP1
XM_003625008.2	XP_003625056.1	triacylglycerol lipase SDP1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_7g090470 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_7g090470 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe173385	XM_003625008.3 Latest version!	Medicago truncatula triacylglycerol lipase SDP1 (LOC11414821), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OMe173385	XM_003625008.2	Medicago truncatula triacylglycerol lipase SDP1 mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_7g090470 ( XM_003625008.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003625056.1 Medicago truncatula triacylglycerol lipase SDP1 (LOC11414821), mRNA.

Price & Availability↑

CloneID	OMe173385
Clone ID Related Accession (Same CDS sequence)	XM_003625008.3 , XM_003625008.2
Accession Version	XM_003625008.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	triacylglycerol lipase SDP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016413.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003625008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCATA TTAGTAATGA AGCTGGGGTA GGCCGTTTTC CAATTGGTCC TTCAGACATT 
CTTGGTAAGA CAATAGCTTT CAGGGTTCTA TTTTGCAAGT CTATGTCACA TTTGAGCTAT 
CAAATATTTC AACTCTTGTT AGGATTCTTG TATAAGTTTA GAGGGGTGTT GAAATTGAAA 
CCCTTTTTAT CATGGTTCCA TCCAAGAAAC CCACAAGGGA TATTAGCATT GATGACAATA 
GTTGCACTCT TTTTGAAACG TTACACAAAT GTGAAAGTTA GGGCTGAAAT GGCTTATAGG 
AGAAAATTTT GGAGAAATAT GATGAGAAGT GCTTTGACCT ATGATGAGTG GGCTCATGGA 
GCTAAGATGC TTGATAAGTT GACTCCAAAG ATGAACGAAT CAGATCTTTA TGACGTTGAA 
TTGGTTGGGA ACAAGCTTCA AGAGCTTCGT AGCCGTCGAC AAGAGGGGTC TCTTAGAGAT 
ATTATGTTTT GTATGCGTGC TGATCTTGTT CGAAATTTAG GTAATATGTG TAACCCGGAA 
CTACACAAGG GTAGGCTTCA AGTCCCTAAG TTAATCAAGG AGTACCTTGA TGAGGTAACG 
ACGCAATTGA GAATGGTTTG TGACTCGAAT TCGGAGGAGT TATCATTGGA AGAAAAGCTT 
GCGTTCATGC ATGAAACAAG ACATGCTTTT GGGAGAACAG CTTTGCTTTT AAGTGGTGGT 
GCTTCTCTTG GAGCTTTTCA TGTTGGTGTA GTGAAGACAT TGGTAAAACA TAAACTCTTG 
CCTAGGATAA TTGCTGGTTC AAGTGTTGGA TCTATTATAA GTGCTATTGT GGCCACTAGA 
TCATGGCCAG AGCTTCAGAG TTTCTTCGAG GATTCGTTGC ATTCGTTGCA ATTTTTTGAT 
CAAATGGGTG GGATTTTCGC GGTTGTCAAG AGGGTTGCAA CACGCGGCGC TGTTCATGAG 
ATCAGGCAGT TGCAAATGTT GCTGAGGCAT TTAACAAACA ACCTTACATT TCAAGAAGCT 
TATGATATGA CAGGTAGAGT TCTTGGAATT ACGGTTTGCT CTCCAAGGAA GCACGAACCT 
CCTAGATGTC TTAACTACTT GACATCGCCT CATGTCGTTA TATGGAGTGC GGTGACAGCT 
TCTTGCGCAT TCCCTGGCCT TTTTGAAGCT CAAGAATTGA TGGCAAAGAA TAGAAGTGGA 
GATATTGTTC CTTATCATCC TCCTTTTAAT CTAGGTCCTG AAGAAGGCTC CACGCCGTCG 
CGTCGTTGGA GGGATGGTAG CTTAGAGATT GACTTACCTA TGATGCAGTT GAAAGAGCTA 
TTCAATGTCA ATCATTTCAT TGTCAGTCAG GCCAACCCTC ACATTGCACC ATTGTTGAGA 
TTGAAAGAGT TTGTAAGAAC TTATGGAGGC AATTTCGCTG CCAAGCTTGC TCACCTTGTG 
GTGTTGGAGG TGAAACATAG GTGTAATCAA GTACTAGAAC TTGGCTTTCC CTTAGGCGGA 
CTTGCCAAAC TGTTTGCTCA AGAATGGGAA GGTGATGTCA CTATTGTTAT GCCTGCTACC 
CTTGCTCAGT ACTCAAAAAT TATACAGAAC CCTTCGTATG GCGAGCTTCA AAAAGCAGCC 
AACCAAGGGA GAAGATGTAG CTGGGAGAAG CTTTCAGCCA TAAAAGCAAA TTGTGAAATT 
GAACTTGCTC TTGATGAATC TGTGGCAATT CTCAATCATA TGAGAAGACT AAGAAGAATT 
GCTGAAAGAA CTTCTGCTTC AGTTTCTCCC AGTTTATCTA GTACTGTCAA ATTCAGTGCT 
TCAAGAAGAA TTCCTTCATG GAATTGCATG GCACGAGAGA ATTCATCAGG CTCTCTTGAA 
GACCTTACAG AAGCTTCTTC CTCGTTGCAT CAAACCATTG GCAGTGACTC TGAAAATGTT 
GATTTGATTT CTTGGACTAA ATCTGGTGGC CCTTTGATGA GAACCGCTTC GGCAAATACG 
TTTGTTGACT TTCTCCGAAA CTTAGAGGTT GATACCGAAC TAAATAGAGG CTCTGTAGCT 
TATGCCAGCC CCCGTGATTT TCAGTATCAT AGTTTCAGAC TCACAGGACC AGATAGGAAC 
TCGGAATCCG AGCAGAAGGA AATTGACAAC AGGGTTGTGA ATGGATCTAG CATACTGGTA 
ACTGAAGGCG ATCTTTTGCA GACTGAAAAG ATTCCTAATG GGATTGTGTT TAATGTTGTC 
AAGAAAGAAG CCTTAACGCC TTCAAATAGG TGTCTTGATT TTGGAAATTA CAACAATGAA 
GTTGTTGAAT GTGATCAAAT TGGTTGTCCA GGGAAGGAAA CCGATACTGT TAGCTCTGAT 
TCTGACCATG AAAATGACGA ATCCGCACCA GCTTTGAGCA TGGATCAAAG CATTGTTGAT 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003625056.1
CDS	555..2960
Translation	MDHISNEAGVGRFPIGPSDILGKTIAFRVLFCKSMSHLSYQIFQLLLGFLYKFRGVLKLKPFLSWFHPRNPQGILALMTIVALFLKRYTNVKVRAEMAYRRKFWRNMMRSALTYDEWAHGAKMLDKLTPKMNESDLYDVELVGNKLQELRSRRQEGSLRDIMFCMRADLVRNLGNMCNPELHKGRLQVPKLIKEYLDEVTTQLRMVCDSNSEELSLEEKLAFMHETRHAFGRTALLLSGGASLGAFHVGVVKTLVKHKLLPRIIAGSSVGSIISAIVATRSWPELQSFFEDSLHSLQFFDQMGGIFAVVKRVATRGAVHEIRQLQMLLRHLTNNLTFQEAYDMTGRVLGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGDIVPYHPPFNLGPEEGSTPSRRWRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKEFVRTYGGNFAAKLAHLVVLEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTIVMPATLAQYSKIIQNPSYGELQKAANQGRRCSWEKLSAIKANCEIELALDESVAILNHMRRLRRIAERTSASVSPSLSSTVKFSASRRIPSWNCMARENSSGSLEDLTEASSSLHQTIGSDSENVDLISWTKSGGPLMRTASANTFVDFLRNLEVDTELNRGSVAYASPRDFQYHSFRLTGPDRNSESEQKEIDNRVVNGSSILVTEGDLLQTEKIPNGIVFNVVKKEALTPSNRCLDFGNYNNEVVECDQIGCPGKETDTVSSDSDHENDESAPALSMDQSIVDS

Target ORF information:

RefSeq Version	XM_003625008.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula triacylglycerol lipase SDP1 (LOC11414821), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003625008.3

ORF Insert Sequence:

ATGGATCATA TTAGTAATGA AGCTGGGGTA GGCCGTTTTC CAATTGGTCC TTCAGACATT 
CTTGGTAAGA CAATAGCTTT CAGGGTTCTA TTTTGCAAGT CTATGTCACA TTTGAGCTAT 
CAAATATTTC AACTCTTGTT AGGATTCTTG TATAAGTTTA GAGGGGTGTT GAAATTGAAA 
CCCTTTTTAT CATGGTTCCA TCCAAGAAAC CCACAAGGGA TATTAGCATT GATGACAATA 
GTTGCACTCT TTTTGAAACG TTACACAAAT GTGAAAGTTA GGGCTGAAAT GGCTTATAGG 
AGAAAATTTT GGAGAAATAT GATGAGAAGT GCTTTGACCT ATGATGAGTG GGCTCATGGA 
GCTAAGATGC TTGATAAGTT GACTCCAAAG ATGAACGAAT CAGATCTTTA TGACGTTGAA 
TTGGTTGGGA ACAAGCTTCA AGAGCTTCGT AGCCGTCGAC AAGAGGGGTC TCTTAGAGAT 
ATTATGTTTT GTATGCGTGC TGATCTTGTT CGAAATTTAG GTAATATGTG TAACCCGGAA 
CTACACAAGG GTAGGCTTCA AGTCCCTAAG TTAATCAAGG AGTACCTTGA TGAGGTAACG 
ACGCAATTGA GAATGGTTTG TGACTCGAAT TCGGAGGAGT TATCATTGGA AGAAAAGCTT 
GCGTTCATGC ATGAAACAAG ACATGCTTTT GGGAGAACAG CTTTGCTTTT AAGTGGTGGT 
GCTTCTCTTG GAGCTTTTCA TGTTGGTGTA GTGAAGACAT TGGTAAAACA TAAACTCTTG 
CCTAGGATAA TTGCTGGTTC AAGTGTTGGA TCTATTATAA GTGCTATTGT GGCCACTAGA 
TCATGGCCAG AGCTTCAGAG TTTCTTCGAG GATTCGTTGC ATTCGTTGCA ATTTTTTGAT 
CAAATGGGTG GGATTTTCGC GGTTGTCAAG AGGGTTGCAA CACGCGGCGC TGTTCATGAG 
ATCAGGCAGT TGCAAATGTT GCTGAGGCAT TTAACAAACA ACCTTACATT TCAAGAAGCT 
TATGATATGA CAGGTAGAGT TCTTGGAATT ACGGTTTGCT CTCCAAGGAA GCACGAACCT 
CCTAGATGTC TTAACTACTT GACATCGCCT CATGTCGTTA TATGGAGTGC GGTGACAGCT 
TCTTGCGCAT TCCCTGGCCT TTTTGAAGCT CAAGAATTGA TGGCAAAGAA TAGAAGTGGA 
GATATTGTTC CTTATCATCC TCCTTTTAAT CTAGGTCCTG AAGAAGGCTC CACGCCGTCG 
CGTCGTTGGA GGGATGGTAG CTTAGAGATT GACTTACCTA TGATGCAGTT GAAAGAGCTA 
TTCAATGTCA ATCATTTCAT TGTCAGTCAG GCCAACCCTC ACATTGCACC ATTGTTGAGA 
TTGAAAGAGT TTGTAAGAAC TTATGGAGGC AATTTCGCTG CCAAGCTTGC TCACCTTGTG 
GTGTTGGAGG TGAAACATAG GTGTAATCAA GTACTAGAAC TTGGCTTTCC CTTAGGCGGA 
CTTGCCAAAC TGTTTGCTCA AGAATGGGAA GGTGATGTCA CTATTGTTAT GCCTGCTACC 
CTTGCTCAGT ACTCAAAAAT TATACAGAAC CCTTCGTATG GCGAGCTTCA AAAAGCAGCC 
AACCAAGGGA GAAGATGTAG CTGGGAGAAG CTTTCAGCCA TAAAAGCAAA TTGTGAAATT 
GAACTTGCTC TTGATGAATC TGTGGCAATT CTCAATCATA TGAGAAGACT AAGAAGAATT 
GCTGAAAGAA CTTCTGCTTC AGTTTCTCCC AGTTTATCTA GTACTGTCAA ATTCAGTGCT 
TCAAGAAGAA TTCCTTCATG GAATTGCATG GCACGAGAGA ATTCATCAGG CTCTCTTGAA 
GACCTTACAG AAGCTTCTTC CTCGTTGCAT CAAACCATTG GCAGTGACTC TGAAAATGTT 
GATTTGATTT CTTGGACTAA ATCTGGTGGC CCTTTGATGA GAACCGCTTC GGCAAATACG 
TTTGTTGACT TTCTCCGAAA CTTAGAGGTT GATACCGAAC TAAATAGAGG CTCTGTAGCT 
TATGCCAGCC CCCGTGATTT TCAGTATCAT AGTTTCAGAC TCACAGGACC AGATAGGAAC 
TCGGAATCCG AGCAGAAGGA AATTGACAAC AGGGTTGTGA ATGGATCTAG CATACTGGTA 
ACTGAAGGCG ATCTTTTGCA GACTGAAAAG ATTCCTAATG GGATTGTGTT TAATGTTGTC 
AAGAAAGAAG CCTTAACGCC TTCAAATAGG TGTCTTGATT TTGGAAATTA CAACAATGAA 
GTTGTTGAAT GTGATCAAAT TGGTTGTCCA GGGAAGGAAA CCGATACTGT TAGCTCTGAT 
TCTGACCATG AAAATGACGA ATCCGCACCA GCTTTGAGCA TGGATCAAAG CATTGTTGAT 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_7g090470 ( XM_003625008.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003625056.1 Medicago truncatula triacylglycerol lipase SDP1 mRNA.

Price & Availability↑

CloneID	OMe173385
Clone ID Related Accession (Same CDS sequence)	XM_003625008.3 , XM_003625008.2
Accession Version	XM_003625008.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	triacylglycerol lipase SDP1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016413). On Aug 25, 2015 this sequence version replaced XM_003625008.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGATCATA TTAGTAATGA AGCTGGGGTA GGCCGTTTTC CAATTGGTCC TTCAGACATT 
CTTGGTAAGA CAATAGCTTT CAGGGTTCTA TTTTGCAAGT CTATGTCACA TTTGAGCTAT 
CAAATATTTC AACTCTTGTT AGGATTCTTG TATAAGTTTA GAGGGGTGTT GAAATTGAAA 
CCCTTTTTAT CATGGTTCCA TCCAAGAAAC CCACAAGGGA TATTAGCATT GATGACAATA 
GTTGCACTCT TTTTGAAACG TTACACAAAT GTGAAAGTTA GGGCTGAAAT GGCTTATAGG 
AGAAAATTTT GGAGAAATAT GATGAGAAGT GCTTTGACCT ATGATGAGTG GGCTCATGGA 
GCTAAGATGC TTGATAAGTT GACTCCAAAG ATGAACGAAT CAGATCTTTA TGACGTTGAA 
TTGGTTGGGA ACAAGCTTCA AGAGCTTCGT AGCCGTCGAC AAGAGGGGTC TCTTAGAGAT 
ATTATGTTTT GTATGCGTGC TGATCTTGTT CGAAATTTAG GTAATATGTG TAACCCGGAA 
CTACACAAGG GTAGGCTTCA AGTCCCTAAG TTAATCAAGG AGTACCTTGA TGAGGTAACG 
ACGCAATTGA GAATGGTTTG TGACTCGAAT TCGGAGGAGT TATCATTGGA AGAAAAGCTT 
GCGTTCATGC ATGAAACAAG ACATGCTTTT GGGAGAACAG CTTTGCTTTT AAGTGGTGGT 
GCTTCTCTTG GAGCTTTTCA TGTTGGTGTA GTGAAGACAT TGGTAAAACA TAAACTCTTG 
CCTAGGATAA TTGCTGGTTC AAGTGTTGGA TCTATTATAA GTGCTATTGT GGCCACTAGA 
TCATGGCCAG AGCTTCAGAG TTTCTTCGAG GATTCGTTGC ATTCGTTGCA ATTTTTTGAT 
CAAATGGGTG GGATTTTCGC GGTTGTCAAG AGGGTTGCAA CACGCGGCGC TGTTCATGAG 
ATCAGGCAGT TGCAAATGTT GCTGAGGCAT TTAACAAACA ACCTTACATT TCAAGAAGCT 
TATGATATGA CAGGTAGAGT TCTTGGAATT ACGGTTTGCT CTCCAAGGAA GCACGAACCT 
CCTAGATGTC TTAACTACTT GACATCGCCT CATGTCGTTA TATGGAGTGC GGTGACAGCT 
TCTTGCGCAT TCCCTGGCCT TTTTGAAGCT CAAGAATTGA TGGCAAAGAA TAGAAGTGGA 
GATATTGTTC CTTATCATCC TCCTTTTAAT CTAGGTCCTG AAGAAGGCTC CACGCCGTCG 
CGTCGTTGGA GGGATGGTAG CTTAGAGATT GACTTACCTA TGATGCAGTT GAAAGAGCTA 
TTCAATGTCA ATCATTTCAT TGTCAGTCAG GCCAACCCTC ACATTGCACC ATTGTTGAGA 
TTGAAAGAGT TTGTAAGAAC TTATGGAGGC AATTTCGCTG CCAAGCTTGC TCACCTTGTG 
GTGTTGGAGG TGAAACATAG GTGTAATCAA GTACTAGAAC TTGGCTTTCC CTTAGGCGGA 
CTTGCCAAAC TGTTTGCTCA AGAATGGGAA GGTGATGTCA CTATTGTTAT GCCTGCTACC 
CTTGCTCAGT ACTCAAAAAT TATACAGAAC CCTTCGTATG GCGAGCTTCA AAAAGCAGCC 
AACCAAGGGA GAAGATGTAG CTGGGAGAAG CTTTCAGCCA TAAAAGCAAA TTGTGAAATT 
GAACTTGCTC TTGATGAATC TGTGGCAATT CTCAATCATA TGAGAAGACT AAGAAGAATT 
GCTGAAAGAA CTTCTGCTTC AGTTTCTCCC AGTTTATCTA GTACTGTCAA ATTCAGTGCT 
TCAAGAAGAA TTCCTTCATG GAATTGCATG GCACGAGAGA ATTCATCAGG CTCTCTTGAA 
GACCTTACAG AAGCTTCTTC CTCGTTGCAT CAAACCATTG GCAGTGACTC TGAAAATGTT 
GATTTGATTT CTTGGACTAA ATCTGGTGGC CCTTTGATGA GAACCGCTTC GGCAAATACG 
TTTGTTGACT TTCTCCGAAA CTTAGAGGTT GATACCGAAC TAAATAGAGG CTCTGTAGCT 
TATGCCAGCC CCCGTGATTT TCAGTATCAT AGTTTCAGAC TCACAGGACC AGATAGGAAC 
TCGGAATCCG AGCAGAAGGA AATTGACAAC AGGGTTGTGA ATGGATCTAG CATACTGGTA 
ACTGAAGGCG ATCTTTTGCA GACTGAAAAG ATTCCTAATG GGATTGTGTT TAATGTTGTC 
AAGAAAGAAG CCTTAACGCC TTCAAATAGG TGTCTTGATT TTGGAAATTA CAACAATGAA 
GTTGTTGAAT GTGATCAAAT TGGTTGTCCA GGGAAGGAAA CCGATACTGT TAGCTCTGAT 
TCTGACCATG AAAATGACGA ATCCGCACCA GCTTTGAGCA TGGATCAAAG CATTGTTGAT 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003625056.1
CDS	593..2998
Translation	MDHISNEAGVGRFPIGPSDILGKTIAFRVLFCKSMSHLSYQIFQLLLGFLYKFRGVLKLKPFLSWFHPRNPQGILALMTIVALFLKRYTNVKVRAEMAYRRKFWRNMMRSALTYDEWAHGAKMLDKLTPKMNESDLYDVELVGNKLQELRSRRQEGSLRDIMFCMRADLVRNLGNMCNPELHKGRLQVPKLIKEYLDEVTTQLRMVCDSNSEELSLEEKLAFMHETRHAFGRTALLLSGGASLGAFHVGVVKTLVKHKLLPRIIAGSSVGSIISAIVATRSWPELQSFFEDSLHSLQFFDQMGGIFAVVKRVATRGAVHEIRQLQMLLRHLTNNLTFQEAYDMTGRVLGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKNRSGDIVPYHPPFNLGPEEGSTPSRRWRDGSLEIDLPMMQLKELFNVNHFIVSQANPHIAPLLRLKEFVRTYGGNFAAKLAHLVVLEVKHRCNQVLELGFPLGGLAKLFAQEWEGDVTIVMPATLAQYSKIIQNPSYGELQKAANQGRRCSWEKLSAIKANCEIELALDESVAILNHMRRLRRIAERTSASVSPSLSSTVKFSASRRIPSWNCMARENSSGSLEDLTEASSSLHQTIGSDSENVDLISWTKSGGPLMRTASANTFVDFLRNLEVDTELNRGSVAYASPRDFQYHSFRLTGPDRNSESEQKEIDNRVVNGSSILVTEGDLLQTEKIPNGIVFNVVKKEALTPSNRCLDFGNYNNEVVECDQIGCPGKETDTVSSDSDHENDESAPALSMDQSIVDS

Target ORF information:

RefSeq Version	XM_003625008.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula triacylglycerol lipase SDP1 mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003625008.2

ORF Insert Sequence:

ATGGATCATA TTAGTAATGA AGCTGGGGTA GGCCGTTTTC CAATTGGTCC TTCAGACATT 
CTTGGTAAGA CAATAGCTTT CAGGGTTCTA TTTTGCAAGT CTATGTCACA TTTGAGCTAT 
CAAATATTTC AACTCTTGTT AGGATTCTTG TATAAGTTTA GAGGGGTGTT GAAATTGAAA 
CCCTTTTTAT CATGGTTCCA TCCAAGAAAC CCACAAGGGA TATTAGCATT GATGACAATA 
GTTGCACTCT TTTTGAAACG TTACACAAAT GTGAAAGTTA GGGCTGAAAT GGCTTATAGG 
AGAAAATTTT GGAGAAATAT GATGAGAAGT GCTTTGACCT ATGATGAGTG GGCTCATGGA 
GCTAAGATGC TTGATAAGTT GACTCCAAAG ATGAACGAAT CAGATCTTTA TGACGTTGAA 
TTGGTTGGGA ACAAGCTTCA AGAGCTTCGT AGCCGTCGAC AAGAGGGGTC TCTTAGAGAT 
ATTATGTTTT GTATGCGTGC TGATCTTGTT CGAAATTTAG GTAATATGTG TAACCCGGAA 
CTACACAAGG GTAGGCTTCA AGTCCCTAAG TTAATCAAGG AGTACCTTGA TGAGGTAACG 
ACGCAATTGA GAATGGTTTG TGACTCGAAT TCGGAGGAGT TATCATTGGA AGAAAAGCTT 
GCGTTCATGC ATGAAACAAG ACATGCTTTT GGGAGAACAG CTTTGCTTTT AAGTGGTGGT 
GCTTCTCTTG GAGCTTTTCA TGTTGGTGTA GTGAAGACAT TGGTAAAACA TAAACTCTTG 
CCTAGGATAA TTGCTGGTTC AAGTGTTGGA TCTATTATAA GTGCTATTGT GGCCACTAGA 
TCATGGCCAG AGCTTCAGAG TTTCTTCGAG GATTCGTTGC ATTCGTTGCA ATTTTTTGAT 
CAAATGGGTG GGATTTTCGC GGTTGTCAAG AGGGTTGCAA CACGCGGCGC TGTTCATGAG 
ATCAGGCAGT TGCAAATGTT GCTGAGGCAT TTAACAAACA ACCTTACATT TCAAGAAGCT 
TATGATATGA CAGGTAGAGT TCTTGGAATT ACGGTTTGCT CTCCAAGGAA GCACGAACCT 
CCTAGATGTC TTAACTACTT GACATCGCCT CATGTCGTTA TATGGAGTGC GGTGACAGCT 
TCTTGCGCAT TCCCTGGCCT TTTTGAAGCT CAAGAATTGA TGGCAAAGAA TAGAAGTGGA 
GATATTGTTC CTTATCATCC TCCTTTTAAT CTAGGTCCTG AAGAAGGCTC CACGCCGTCG 
CGTCGTTGGA GGGATGGTAG CTTAGAGATT GACTTACCTA TGATGCAGTT GAAAGAGCTA 
TTCAATGTCA ATCATTTCAT TGTCAGTCAG GCCAACCCTC ACATTGCACC ATTGTTGAGA 
TTGAAAGAGT TTGTAAGAAC TTATGGAGGC AATTTCGCTG CCAAGCTTGC TCACCTTGTG 
GTGTTGGAGG TGAAACATAG GTGTAATCAA GTACTAGAAC TTGGCTTTCC CTTAGGCGGA 
CTTGCCAAAC TGTTTGCTCA AGAATGGGAA GGTGATGTCA CTATTGTTAT GCCTGCTACC 
CTTGCTCAGT ACTCAAAAAT TATACAGAAC CCTTCGTATG GCGAGCTTCA AAAAGCAGCC 
AACCAAGGGA GAAGATGTAG CTGGGAGAAG CTTTCAGCCA TAAAAGCAAA TTGTGAAATT 
GAACTTGCTC TTGATGAATC TGTGGCAATT CTCAATCATA TGAGAAGACT AAGAAGAATT 
GCTGAAAGAA CTTCTGCTTC AGTTTCTCCC AGTTTATCTA GTACTGTCAA ATTCAGTGCT 
TCAAGAAGAA TTCCTTCATG GAATTGCATG GCACGAGAGA ATTCATCAGG CTCTCTTGAA 
GACCTTACAG AAGCTTCTTC CTCGTTGCAT CAAACCATTG GCAGTGACTC TGAAAATGTT 
GATTTGATTT CTTGGACTAA ATCTGGTGGC CCTTTGATGA GAACCGCTTC GGCAAATACG 
TTTGTTGACT TTCTCCGAAA CTTAGAGGTT GATACCGAAC TAAATAGAGG CTCTGTAGCT 
TATGCCAGCC CCCGTGATTT TCAGTATCAT AGTTTCAGAC TCACAGGACC AGATAGGAAC 
TCGGAATCCG AGCAGAAGGA AATTGACAAC AGGGTTGTGA ATGGATCTAG CATACTGGTA 
ACTGAAGGCG ATCTTTTGCA GACTGAAAAG ATTCCTAATG GGATTGTGTT TAATGTTGTC 
AAGAAAGAAG CCTTAACGCC TTCAAATAGG TGTCTTGATT TTGGAAATTA CAACAATGAA 
GTTGTTGAAT GTGATCAAAT TGGTTGTCCA GGGAAGGAAA CCGATACTGT TAGCTCTGAT 
TCTGACCATG AAAATGACGA ATCCGCACCA GCTTTGAGCA TGGATCAAAG CATTGTTGAT 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_7g090470 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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