GCC2 cDNA ORF clone, Manacus vitellinus(golden-collared manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCC2
Entrez Gene ID	103756129
Full Name	GRIP and coiled-coil domain containing 2
Gene Type	protein-coding
Organism	Manacus vitellinus(golden-collared manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018084170.1	XP_017939659.1	GRIP and coiled-coil domain-containing protein 2 isoform X2
XM_018084173.1	XP_017939662.1	GRIP and coiled-coil domain-containing protein 2 isoform X5
XM_018084169.1	XP_017939658.1	GRIP and coiled-coil domain-containing protein 2 isoform X1
XM_018084171.1	XP_017939660.1	GRIP and coiled-coil domain-containing protein 2 isoform X2
XM_018084172.2	XP_017939661.1	GRIP and coiled-coil domain-containing protein 2 isoform X3
XM_018084170.2	XP_017939659.1	GRIP and coiled-coil domain-containing protein 2 isoform X1
XM_018084173.2	XP_017939662.1	GRIP and coiled-coil domain-containing protein 2 isoform X4
XM_018084172.1	XP_017939661.1	GRIP and coiled-coil domain-containing protein 2 isoform X4

Pathways from BioSystems
Gene Ontology

The following GCC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa214686	XM_018084170.2 Latest version!	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa214687	XM_018084171.1 Latest version!	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa214688	XM_018084172.2 Latest version!	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa214689	XM_018084173.2 Latest version!	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa214685	XM_018084169.1 Latest version!	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OMa214686	XM_018084170.1	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa214689	XM_018084173.1	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OMa214688	XM_018084172.1	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCC2 ( XM_018084170.1 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939659.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

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CloneID	OMa214686
Clone ID Related Accession (Same CDS sequence)	XM_018084170.1 , XM_018084170.2
Accession Version	XM_018084170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-24
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020340930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGTGCCGT GTTTGTTAGA ATGTTTCCTA GAAAATGTCA CCCTTGCCCA AAGAGTTTAT 
AAGCTTAGGA CCAATTTTTA TCTTCTATCA GCCTGCCTTC AGCCCAAGCC TAAGGAGGGC 
ATAATAATAA TAATAATAAT AATAAAGATT CTGCTGTCAC TATGCAGAGT ATATTTATTA 
ATTAAAAACC AAAAATGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939659.1
CDS	39..5282
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRETFSGDTAACCISPQIPSPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQVPCLLECFLENVTLAQRVYKLRTNFYLLSACLQPKPKEGIIIIIIIIKILLSLCRVYLLIKNQKWE

Target ORF information:

RefSeq Version	XM_018084170.1
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084170.1

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGTGCCGT GTTTGTTAGA ATGTTTCCTA GAAAATGTCA CCCTTGCCCA AAGAGTTTAT 
AAGCTTAGGA CCAATTTTTA TCTTCTATCA GCCTGCCTTC AGCCCAAGCC TAAGGAGGGC 
ATAATAATAA TAATAATAAT AATAAAGATT CTGCTGTCAC TATGCAGAGT ATATTTATTA 
ATTAAAAACC AAAAATGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084170.2 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939659.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

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CloneID	OMa214686
Clone ID Related Accession (Same CDS sequence)	XM_018084170.1 , XM_018084170.2
Accession Version	XM_018084170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021940813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 19, 2019 this sequence version replaced XM_018084170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGTGCCGT GTTTGTTAGA ATGTTTCCTA GAAAATGTCA CCCTTGCCCA AAGAGTTTAT 
AAGCTTAGGA CCAATTTTTA TCTTCTATCA GCCTGCCTTC AGCCCAAGCC TAAGGAGGGC 
ATAATAATAA TAATAATAAT AATAAAGATT CTGCTGTCAC TATGCAGAGT ATATTTATTA 
ATTAAAAACC AAAAATGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939659.1
CDS	32..5275
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRETFSGDTAACCISPQIPSPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQVPCLLECFLENVTLAQRVYKLRTNFYLLSACLQPKPKEGIIIIIIIIKILLSLCRVYLLIKNQKWE

Target ORF information:

RefSeq Version	XM_018084170.2
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084170.2

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGTGCCGT GTTTGTTAGA ATGTTTCCTA GAAAATGTCA CCCTTGCCCA AAGAGTTTAT 
AAGCTTAGGA CCAATTTTTA TCTTCTATCA GCCTGCCTTC AGCCCAAGCC TAAGGAGGGC 
ATAATAATAA TAATAATAAT AATAAAGATT CTGCTGTCAC TATGCAGAGT ATATTTATTA 
ATTAAAAACC AAAAATGGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084171.1 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939660.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa214687
Clone ID Related Accession (Same CDS sequence)	XM_018084171.1
Accession Version	XM_018084171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021940813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGT CTTCTGGCAC ACCAGGATCA GGCAAATCAA AGCTGGACAC ATTGCCCAAA 
GAAGACCTCA TTAAGTTTGC AAAAAAGCAG ATGATGCTTA TGCAGAAAGT GAAGTCAAGA 
TGTACAGAAC TGGAGAAAGA GATTGAAGAA CTCAGATCTA AAGCAGCTAC TGCAGGAGCT 
GATGATATTA TTCAGGCTCT CACAGAAAGA CTGGACGTTG TACTTCTGGA AAAAGCTGAA 
AGTCAGCAGC AGTGCATTGC TCTGAGAAAA GAAAATATTC AAATAAAGCA AGAAGGGGAG 
GCTGCAGTGG CTAAGACAGA AGAATTGCAG AAAATACTGG AACAATCAAG TATTGACTCT 
CTGGAAGAAA TAAAAGCTTT GAAGTGTGAA TTAGCAAATG CACAATGCAA GCACAATGAG 
GATTTAAAAA AGCTGAAAAT GGAATTAGAT GAACAAGTGA AAAAACAAAT GGAGCTGATG 
GAACAGGTTG AACGTCATAG TGACAGTCAA AAAGAAGTTA CAAGATTACA AGATGATGTC 
CAGAGAATGA AATCTACCTA TGAGGAGCAA ATTTTGTGTC TGAGCAAGCA GTTGGAAACT 
GTGACCGAAG ACAAAACCAA AGAAGTGACA AGTCTGCAAG AAACTATTAA AAACAACTCT 
CAATGTTACC ATAATGAAAT AAAAAATCTG AATGAAGAGC TTAAAAAATT AAAAATTGCC 
CATCAGGAAG AGGTGTCAGA ATTGATGCAT CGGATTGAAA TATCATCTAA AGAAAATGAA 
GAAAAGCAAA ATCAAATAAA TGAGTTACAG CACAATTTGG CAGAGAAAAA TGCAAAGGAT 
GAAATGCATT GTCATACTGA CCAGCGCGAA TACGACTTGG AGCAGCTGAG AGAAGTCTTA 
AATAAAAATG TAGAAAACAA GATAGATATT GCAGATACAC CAGAAGAACC TTACATAGAA 
GCAAAAATGG AAGCAAAGGT TAGGTACCTG GAGCATAGTT TAGAAGAACT CCAGTCCCAA 
CATAGTATAT TAAAGGATGA GTTAACTTAC ATGAGCAATG TCAAACTAAA GCTGGAGGAG 
GAAATCCATC GCATAAAGGA TGAGTACTTT CATGAGCGGG AAGATTTGGA CTTTAAGATA 
AATGAATTGC AGCTTGCTAA AGAAGATTAC TGCTGTGTAA TTGAAAAACT AAAATTGGAG 
CTTAAAGCAG CAAGACAGCA CTATGAAGCT GATTTAGAAG AGCATAAATT AGAGATTCAA 
ACTCTGAAAG AGCAACACAA GACAGAAATT TCTGAATTAA ATGAAACTTT ATTATCTAGT 
TCTGAAAAAG AAAAGATGGT ATTAGTTTTT GAAATACAGG AACTTAGAGA ACAACTTGAA 
AAGCTAACTC AGGAGAAAGA AGAAGCAGTG TCCAATTACA ATAGCCTGAG AGAAACAATG 
GAAACTCTCC AGGCTGAGCT AGGCGAATCT GCTGGAAAGA TCAGCCAGGA GTTTGAATCA 
ATGAAACAAC AGCAAGCTTC TGATGTCAGT CAACTGCAAC AGAAACTCCG AGCTGCTTTC 
AATGAAAGGG ATGTTCTTCT TGAAACTGTA AATCGCCTCC AGGAAGAAAT AGAAAAATTG 
CCATCTAATC AGCTAGAGAT AGAAGAACTT AAATGCAAAA TTGTTAGTCT TCAGGAGGAC 
AATAACGCAA TAAGAAGCTC CATCAACCAA AAAGAGATTA CTGTAAAAGA ACTGGAAGAG 
AAGATAGTTG CTCTTACTGA TCAAAACAAG GATATTTTAA ATGATGTAAA ATGTTTGGGT 
GAAGAGAGAG AAACCCTTCA GGAAAGGTGC AAGCAAGAAC AAGTTAAAAT TCAGGAACTT 
CAGCAAGAAG TAGATGTTGC TAACCAGAAC AATAATGACC TGACGAAAAA GGTAGAGGAA 
TTAACAGAGA GACTGAATGA AGCTTTAACT ACAAAGACTG AAAATGCTCA AATGCTAGAG 
CAACTGGAAA AACAGATTGA ATCTCTGGTA CATGATAGAG AGCAGCTTTC ATCTGAAGTG 
TATACTCTTC ATGAGGAAAA TAAGAAAATC ATTCAGGAAA AAGGTGAATT AAGTGAAGAG 
CTGGGAAAGA TTACATCTGA AAAAGATGGT TGGTTAGTGT TGAAAGAGCA GTCTGAAAAC 
TTAGAAAAGA AACTACAAAC GATGACTGCA GAAAAAGACC ATGTATCAAC ATTACTCGAA 
AATGAACAAA GGCACACGTC CCTTGTAAGA ACTCAACTGT ACCACCTAGT TGAGAAAATG 
GGGTCCGACA TTTCAGAGTC TAATGAAGAA TATGATTCTG TTAACTTGTT AAAAATTGCA 
AATGAATGCC TGTCAAAAAT TAAAGAAAAG CAGTGCCTTG CTCTACAAAA TGAGGAGGAA 
GTAATTCATT TGCAGCGGGA AGTTGAGAGA CTAGAGGAAG AACATGCTGC TCAATATAGA 
GAACATAGAT CCCTGATTCA GGATTTTGAA AAAGAAAAGG CTCTCTTGAG AGAGGAACTG 
GAAGGAGTGT TGTCTGAAAA GGAAGCTCTT CAGCATGATA TTCAGGAGCT GAAGAATGCC 
AGTGAGAAAA CAAGGATTGA AAATCAAGAT CTTTTAGCTA ATATTGAAGA GATCACTCAA 
AAACTTGCTT TTTATGAAAG TCCAATGCAA GAACAGGAAA AAGGATCAGA AAAAAAAGAC 
GACTTAAGTT GCATTCTGGA ACAAAAGGAA ACTGAACTTA GAAATGTGAA AGACGAACTG 
AGTTCTCTAA AGAATTTAAT GGAGACAATG ACTGAGAAAA CTGATCAGCA GTCTTCAGTA 
GCAGAACTTC AAGAAAAAAT TGGGAGGCTG GAAAAAGAAT CTGCAGAAAA AGGAGAGAAG 
CTAAATAAAA TTAAGGTGGT TGCTGTGAAA GCAAAAAAGG AATTAGATGC CAGCCGAAAA 
GAGATGCAAG CTCTGAACGA AGAATTGGAG TTGGTTCGAT CAGAAAAAGA TCAGTTGTCT 
GCTTCAATGA AAGACGTTAT TCAAGGAGCT GAAAGCTATA AGAATCTTTT GATGGAATAT 
GATAAGCAAG GAGAACAGCT GGATTCTGAA AAAGGCAGAG CAAATAATCT TGAACGTCAG 
ATAGATGACC TCACAAGACA GCTACAGGTG TCATCCCAGC AGCATGATCA ATTACGCTCT 
GCTAATGAAG ACCTCCTGGC TCGTGTTGAA ACACTGCAAA ATAATGCTAA GCTGCTGGAA 
ACTCAGATAT TGGAGATGCA AAGAGCCAAG GCAAAGGCTG ACAAAGAACT AGAAGCTGAA 
AGGCTTTTAA GAGAACAGAA AACAAAGGAA CATAGTGGTG CTCTCCGAGA AATGGAGGAG 
CTTCAGATGC AACTTCAGAA GGAAAAGAAA CATCTGCAGA AAACTATGTT AGAACTAGAG 
CTGGCCAGAA AGGATGCTCA AAAGAGTACA CTAATGGATA TGGAAATAGC TGATTATGAA 
AGGCTGGTAA AAGAACTTAA TCAGAAGATT ACTGATAAAG ACAGTAAAAT AGAAGATCTT 
GAGCAAGAAA CGAGTATTCA GAAACAGAAG CAAGAGACCC TACAGGAAGA AATAAAATTC 
CTTCAGTCAA CTATGCAACA GGATGAGGAA AGAAATGCCA AGATAAAACA ACTCCTAGTA 
AAAACCAAAA AAGAACTGGC TGATTCAAAA CAAGCTGAAA ATGATCATCT AATGTTGCAG 
GAATCCTTGA AAGGGGAATT GGAAGCCACT CAACAGCAAG TGGAAGCCTA TAAGATTCAA 
GTGGCTGTGC TGACATCAGA AAAGCATAAA GTTCAGGAAC AGCTGCGAAC GTCTTCGGAG 
CAACACCAGC GTATGATGAG TGCTTGCCAG CAAAAAATTG CAACCCTGCA AGAAGAGTGC 
AGAGCAGCCC AGGCTGAACA AGCATCTGTT ACGTCAGAAT TTGAGAGCTA CAAAGTCCGT 
GTTCATAATG TTCTAAAACA GCAAAAGAAT AAATCTGCTG CTCGGACAGA GTCTGAAGGA 
GCCAAACAAG AGAGAGAACA GTTGGAAATG GTGATAGATG AACTTAAAGT TAAGCTGCAA 
GATGCTCAGC ATAACTCACA GATGAATGCA TCTGAACTCC AGGCACTGCA GTCTGAACAC 
GACACCCTGC TGGAAAGACA TAATAAGATG CTGCAAGAGA CTGTTGCAAA AGAAGCAGAA 
CTCCGTGAAA AACTCTGCAC AATACAGTCT GAGAACATGG TCATGAAAAC AGAGCACGCC 
CAGATGTTGA GTCAGCTGAC AGCCCAGAAT GAAGCTCTTC GGAACAGTTT CAGAGATCAA 
GTCAGGAACC TGCAAGAAGA GCACAGGAAA ACAGTAGAGA CACTTCAGCA GCAACTCTCC 
AGAGTAGAAG CTCAGCTCTT CCAACTCAAG AGTGAACCAA GTACTAGAGA AACCTTTTCT 
GGAGACACTG CAGCGTGCTG TATCAGTCCA CAGATACCAA GTCCAGCTGT TTCAAATCCA 
GGAACGAAGA ACTTGAGAGA ACGGCGAAAC ACTGACCTTC CTGTTCTCGA TGTGCACACT 
GTAGCTAGGG AAGAGGGAGA AGGTATGGAA ACAACAGACA CAGAGTCTGT CTCTTCAGCC 
AGCACCTATG TACAATCCTT GGAACAACTT CTGAACTCAT CAGAAGCAAA ACCTGAACCA 
TCTCAGTGGC AAGCAGAACT GACCAAGGAT GAACTGATTC AGAAACTAAA CACAACAGCG 
AAGAGTGCTG ATCACTTAAA TGAATTACTT CGTGAATCAG AAGCAACCAA TGCAATCCTA 
ATGGAACAAA TAAAGCTTCT TAAGAATGAA ATAAGAAGAC TGGAAAGAAA TCAAGAGAGA 
GAAAAGTCTG TGGCTAATTT AGAATACTTG AAGAATGTTC TACTGCAGTT CATATTTCTA 
AAATCAGGAA GCGAGAAAGA GCGGCTGCTC CCAGTAATAG ACACCATGTT GCAGCTCAGC 
CCTGAAGAGA AGGGGAAGCT GGTTGCAATT GCCCAAGTGC CGTGTTTGTT AGAATGTTTC 
CTAGAAAATG TCACCCTTGC CCAAAGAGTT TATAAGCTTA GGACCAATTT TTATCTTCTA 
TCAGCCTGCC TTCAGCCCAA GCCTAAGGAG GGCATAATAA TAATAATAAT AATAATAAAG 
ATTCTGCTGT CACTATGCAG AGTATATTTA TTAATTAAAA ACCAAAAATG GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939660.1
CDS	63..5279
Translation	MVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRETFSGDTAACCISPQIPSPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQVPCLLECFLENVTLAQRVYKLRTNFYLLSACLQPKPKEGIIIIIIIIKILLSLCRVYLLIKNQKWE

Target ORF information:

RefSeq Version	XM_018084171.1
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084171.1

ORF Insert Sequence:

ATGGTGGCGT CTTCTGGCAC ACCAGGATCA GGCAAATCAA AGCTGGACAC ATTGCCCAAA 
GAAGACCTCA TTAAGTTTGC AAAAAAGCAG ATGATGCTTA TGCAGAAAGT GAAGTCAAGA 
TGTACAGAAC TGGAGAAAGA GATTGAAGAA CTCAGATCTA AAGCAGCTAC TGCAGGAGCT 
GATGATATTA TTCAGGCTCT CACAGAAAGA CTGGACGTTG TACTTCTGGA AAAAGCTGAA 
AGTCAGCAGC AGTGCATTGC TCTGAGAAAA GAAAATATTC AAATAAAGCA AGAAGGGGAG 
GCTGCAGTGG CTAAGACAGA AGAATTGCAG AAAATACTGG AACAATCAAG TATTGACTCT 
CTGGAAGAAA TAAAAGCTTT GAAGTGTGAA TTAGCAAATG CACAATGCAA GCACAATGAG 
GATTTAAAAA AGCTGAAAAT GGAATTAGAT GAACAAGTGA AAAAACAAAT GGAGCTGATG 
GAACAGGTTG AACGTCATAG TGACAGTCAA AAAGAAGTTA CAAGATTACA AGATGATGTC 
CAGAGAATGA AATCTACCTA TGAGGAGCAA ATTTTGTGTC TGAGCAAGCA GTTGGAAACT 
GTGACCGAAG ACAAAACCAA AGAAGTGACA AGTCTGCAAG AAACTATTAA AAACAACTCT 
CAATGTTACC ATAATGAAAT AAAAAATCTG AATGAAGAGC TTAAAAAATT AAAAATTGCC 
CATCAGGAAG AGGTGTCAGA ATTGATGCAT CGGATTGAAA TATCATCTAA AGAAAATGAA 
GAAAAGCAAA ATCAAATAAA TGAGTTACAG CACAATTTGG CAGAGAAAAA TGCAAAGGAT 
GAAATGCATT GTCATACTGA CCAGCGCGAA TACGACTTGG AGCAGCTGAG AGAAGTCTTA 
AATAAAAATG TAGAAAACAA GATAGATATT GCAGATACAC CAGAAGAACC TTACATAGAA 
GCAAAAATGG AAGCAAAGGT TAGGTACCTG GAGCATAGTT TAGAAGAACT CCAGTCCCAA 
CATAGTATAT TAAAGGATGA GTTAACTTAC ATGAGCAATG TCAAACTAAA GCTGGAGGAG 
GAAATCCATC GCATAAAGGA TGAGTACTTT CATGAGCGGG AAGATTTGGA CTTTAAGATA 
AATGAATTGC AGCTTGCTAA AGAAGATTAC TGCTGTGTAA TTGAAAAACT AAAATTGGAG 
CTTAAAGCAG CAAGACAGCA CTATGAAGCT GATTTAGAAG AGCATAAATT AGAGATTCAA 
ACTCTGAAAG AGCAACACAA GACAGAAATT TCTGAATTAA ATGAAACTTT ATTATCTAGT 
TCTGAAAAAG AAAAGATGGT ATTAGTTTTT GAAATACAGG AACTTAGAGA ACAACTTGAA 
AAGCTAACTC AGGAGAAAGA AGAAGCAGTG TCCAATTACA ATAGCCTGAG AGAAACAATG 
GAAACTCTCC AGGCTGAGCT AGGCGAATCT GCTGGAAAGA TCAGCCAGGA GTTTGAATCA 
ATGAAACAAC AGCAAGCTTC TGATGTCAGT CAACTGCAAC AGAAACTCCG AGCTGCTTTC 
AATGAAAGGG ATGTTCTTCT TGAAACTGTA AATCGCCTCC AGGAAGAAAT AGAAAAATTG 
CCATCTAATC AGCTAGAGAT AGAAGAACTT AAATGCAAAA TTGTTAGTCT TCAGGAGGAC 
AATAACGCAA TAAGAAGCTC CATCAACCAA AAAGAGATTA CTGTAAAAGA ACTGGAAGAG 
AAGATAGTTG CTCTTACTGA TCAAAACAAG GATATTTTAA ATGATGTAAA ATGTTTGGGT 
GAAGAGAGAG AAACCCTTCA GGAAAGGTGC AAGCAAGAAC AAGTTAAAAT TCAGGAACTT 
CAGCAAGAAG TAGATGTTGC TAACCAGAAC AATAATGACC TGACGAAAAA GGTAGAGGAA 
TTAACAGAGA GACTGAATGA AGCTTTAACT ACAAAGACTG AAAATGCTCA AATGCTAGAG 
CAACTGGAAA AACAGATTGA ATCTCTGGTA CATGATAGAG AGCAGCTTTC ATCTGAAGTG 
TATACTCTTC ATGAGGAAAA TAAGAAAATC ATTCAGGAAA AAGGTGAATT AAGTGAAGAG 
CTGGGAAAGA TTACATCTGA AAAAGATGGT TGGTTAGTGT TGAAAGAGCA GTCTGAAAAC 
TTAGAAAAGA AACTACAAAC GATGACTGCA GAAAAAGACC ATGTATCAAC ATTACTCGAA 
AATGAACAAA GGCACACGTC CCTTGTAAGA ACTCAACTGT ACCACCTAGT TGAGAAAATG 
GGGTCCGACA TTTCAGAGTC TAATGAAGAA TATGATTCTG TTAACTTGTT AAAAATTGCA 
AATGAATGCC TGTCAAAAAT TAAAGAAAAG CAGTGCCTTG CTCTACAAAA TGAGGAGGAA 
GTAATTCATT TGCAGCGGGA AGTTGAGAGA CTAGAGGAAG AACATGCTGC TCAATATAGA 
GAACATAGAT CCCTGATTCA GGATTTTGAA AAAGAAAAGG CTCTCTTGAG AGAGGAACTG 
GAAGGAGTGT TGTCTGAAAA GGAAGCTCTT CAGCATGATA TTCAGGAGCT GAAGAATGCC 
AGTGAGAAAA CAAGGATTGA AAATCAAGAT CTTTTAGCTA ATATTGAAGA GATCACTCAA 
AAACTTGCTT TTTATGAAAG TCCAATGCAA GAACAGGAAA AAGGATCAGA AAAAAAAGAC 
GACTTAAGTT GCATTCTGGA ACAAAAGGAA ACTGAACTTA GAAATGTGAA AGACGAACTG 
AGTTCTCTAA AGAATTTAAT GGAGACAATG ACTGAGAAAA CTGATCAGCA GTCTTCAGTA 
GCAGAACTTC AAGAAAAAAT TGGGAGGCTG GAAAAAGAAT CTGCAGAAAA AGGAGAGAAG 
CTAAATAAAA TTAAGGTGGT TGCTGTGAAA GCAAAAAAGG AATTAGATGC CAGCCGAAAA 
GAGATGCAAG CTCTGAACGA AGAATTGGAG TTGGTTCGAT CAGAAAAAGA TCAGTTGTCT 
GCTTCAATGA AAGACGTTAT TCAAGGAGCT GAAAGCTATA AGAATCTTTT GATGGAATAT 
GATAAGCAAG GAGAACAGCT GGATTCTGAA AAAGGCAGAG CAAATAATCT TGAACGTCAG 
ATAGATGACC TCACAAGACA GCTACAGGTG TCATCCCAGC AGCATGATCA ATTACGCTCT 
GCTAATGAAG ACCTCCTGGC TCGTGTTGAA ACACTGCAAA ATAATGCTAA GCTGCTGGAA 
ACTCAGATAT TGGAGATGCA AAGAGCCAAG GCAAAGGCTG ACAAAGAACT AGAAGCTGAA 
AGGCTTTTAA GAGAACAGAA AACAAAGGAA CATAGTGGTG CTCTCCGAGA AATGGAGGAG 
CTTCAGATGC AACTTCAGAA GGAAAAGAAA CATCTGCAGA AAACTATGTT AGAACTAGAG 
CTGGCCAGAA AGGATGCTCA AAAGAGTACA CTAATGGATA TGGAAATAGC TGATTATGAA 
AGGCTGGTAA AAGAACTTAA TCAGAAGATT ACTGATAAAG ACAGTAAAAT AGAAGATCTT 
GAGCAAGAAA CGAGTATTCA GAAACAGAAG CAAGAGACCC TACAGGAAGA AATAAAATTC 
CTTCAGTCAA CTATGCAACA GGATGAGGAA AGAAATGCCA AGATAAAACA ACTCCTAGTA 
AAAACCAAAA AAGAACTGGC TGATTCAAAA CAAGCTGAAA ATGATCATCT AATGTTGCAG 
GAATCCTTGA AAGGGGAATT GGAAGCCACT CAACAGCAAG TGGAAGCCTA TAAGATTCAA 
GTGGCTGTGC TGACATCAGA AAAGCATAAA GTTCAGGAAC AGCTGCGAAC GTCTTCGGAG 
CAACACCAGC GTATGATGAG TGCTTGCCAG CAAAAAATTG CAACCCTGCA AGAAGAGTGC 
AGAGCAGCCC AGGCTGAACA AGCATCTGTT ACGTCAGAAT TTGAGAGCTA CAAAGTCCGT 
GTTCATAATG TTCTAAAACA GCAAAAGAAT AAATCTGCTG CTCGGACAGA GTCTGAAGGA 
GCCAAACAAG AGAGAGAACA GTTGGAAATG GTGATAGATG AACTTAAAGT TAAGCTGCAA 
GATGCTCAGC ATAACTCACA GATGAATGCA TCTGAACTCC AGGCACTGCA GTCTGAACAC 
GACACCCTGC TGGAAAGACA TAATAAGATG CTGCAAGAGA CTGTTGCAAA AGAAGCAGAA 
CTCCGTGAAA AACTCTGCAC AATACAGTCT GAGAACATGG TCATGAAAAC AGAGCACGCC 
CAGATGTTGA GTCAGCTGAC AGCCCAGAAT GAAGCTCTTC GGAACAGTTT CAGAGATCAA 
GTCAGGAACC TGCAAGAAGA GCACAGGAAA ACAGTAGAGA CACTTCAGCA GCAACTCTCC 
AGAGTAGAAG CTCAGCTCTT CCAACTCAAG AGTGAACCAA GTACTAGAGA AACCTTTTCT 
GGAGACACTG CAGCGTGCTG TATCAGTCCA CAGATACCAA GTCCAGCTGT TTCAAATCCA 
GGAACGAAGA ACTTGAGAGA ACGGCGAAAC ACTGACCTTC CTGTTCTCGA TGTGCACACT 
GTAGCTAGGG AAGAGGGAGA AGGTATGGAA ACAACAGACA CAGAGTCTGT CTCTTCAGCC 
AGCACCTATG TACAATCCTT GGAACAACTT CTGAACTCAT CAGAAGCAAA ACCTGAACCA 
TCTCAGTGGC AAGCAGAACT GACCAAGGAT GAACTGATTC AGAAACTAAA CACAACAGCG 
AAGAGTGCTG ATCACTTAAA TGAATTACTT CGTGAATCAG AAGCAACCAA TGCAATCCTA 
ATGGAACAAA TAAAGCTTCT TAAGAATGAA ATAAGAAGAC TGGAAAGAAA TCAAGAGAGA 
GAAAAGTCTG TGGCTAATTT AGAATACTTG AAGAATGTTC TACTGCAGTT CATATTTCTA 
AAATCAGGAA GCGAGAAAGA GCGGCTGCTC CCAGTAATAG ACACCATGTT GCAGCTCAGC 
CCTGAAGAGA AGGGGAAGCT GGTTGCAATT GCCCAAGTGC CGTGTTTGTT AGAATGTTTC 
CTAGAAAATG TCACCCTTGC CCAAAGAGTT TATAAGCTTA GGACCAATTT TTATCTTCTA 
TCAGCCTGCC TTCAGCCCAA GCCTAAGGAG GGCATAATAA TAATAATAAT AATAATAAAG 
ATTCTGCTGT CACTATGCAG AGTATATTTA TTAATTAAAA ACCAAAAATG GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084172.2 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939661.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.

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CloneID	OMa214688
Clone ID Related Accession (Same CDS sequence)	XM_018084172.2 , XM_018084172.1
Accession Version	XM_018084172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021940813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 19, 2019 this sequence version replaced XM_018084172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGGTGAGG AAGAGAGTAC CTCACGGCCC TCTGGGTGGG CTTCGTACCT CCACAGCTGG 
TCAGGACTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939661.1
CDS	32..5146
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRETFSGDTAACCISPQIPSPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQGEEESTSRPSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_018084172.2
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084172.2

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGGTGAGG AAGAGAGTAC CTCACGGCCC TCTGGGTGGG CTTCGTACCT CCACAGCTGG 
TCAGGACTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084172.1 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939661.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.

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CloneID	OMa214688
Clone ID Related Accession (Same CDS sequence)	XM_018084172.2 , XM_018084172.1
Accession Version	XM_018084172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-24
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020340930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGGTGAGG AAGAGAGTAC CTCACGGCCC TCTGGGTGGG CTTCGTACCT CCACAGCTGG 
TCAGGACTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939661.1
CDS	39..5153
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRETFSGDTAACCISPQIPSPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQGEEESTSRPSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_018084172.1
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084172.1

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGAAAC CTTTTCTGGA GACACTGCAG CGTGCTGTAT CAGTCCACAG 
ATACCAAGTC CAGCTGTTTC AAATCCAGGA ACGAAGAACT TGAGAGAACG GCGAAACACT 
GACCTTCCTG TTCTCGATGT GCACACTGTA GCTAGGGAAG AGGGAGAAGG TATGGAAACA 
ACAGACACAG AGTCTGTCTC TTCAGCCAGC ACCTATGTAC AATCCTTGGA ACAACTTCTG 
AACTCATCAG AAGCAAAACC TGAACCATCT CAGTGGCAAG CAGAACTGAC CAAGGATGAA 
CTGATTCAGA AACTAAACAC AACAGCGAAG AGTGCTGATC ACTTAAATGA ATTACTTCGT 
GAATCAGAAG CAACCAATGC AATCCTAATG GAACAAATAA AGCTTCTTAA GAATGAAATA 
AGAAGACTGG AAAGAAATCA AGAGAGAGAA AAGTCTGTGG CTAATTTAGA ATACTTGAAG 
AATGTTCTAC TGCAGTTCAT ATTTCTAAAA TCAGGAAGCG AGAAAGAGCG GCTGCTCCCA 
GTAATAGACA CCATGTTGCA GCTCAGCCCT GAAGAGAAGG GGAAGCTGGT TGCAATTGCC 
CAAGGTGAGG AAGAGAGTAC CTCACGGCCC TCTGGGTGGG CTTCGTACCT CCACAGCTGG 
TCAGGACTTC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084173.1 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939662.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X5, mRNA.

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CloneID	OMa214689
Clone ID Related Accession (Same CDS sequence)	XM_018084173.1 , XM_018084173.2
Accession Version	XM_018084173.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-24
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020340930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGGTCC AGCTGTTTCA AATCCAGGAA CGAAGAACTT GAGAGAACGG 
CGAAACACTG ACCTTCCTGT TCTCGATGTG CACACTGTAG CTAGGGAAGA GGGAGAAGGT 
ATGGAAACAA CAGACACAGA GTCTGTCTCT TCAGCCAGCA CCTATGTACA ATCCTTGGAA 
CAACTTCTGA ACTCATCAGA AGCAAAACCT GAACCATCTC AGTGGCAAGC AGAACTGACC 
AAGGATGAAC TGATTCAGAA ACTAAACACA ACAGCGAAGA GTGCTGATCA CTTAAATGAA 
TTACTTCGTG AATCAGAAGC AACCAATGCA ATCCTAATGG AACAAATAAA GCTTCTTAAG 
AATGAAATAA GAAGACTGGA AAGAAATCAA GAGAGAGAAA AGTCTGTGGC TAATTTAGAA 
TACTTGAAGA ATGTTCTACT GCAGTTCATA TTTCTAAAAT CAGGAAGCGA GAAAGAGCGG 
CTGCTCCCAG TAATAGACAC CATGTTGCAG CTCAGCCCTG AAGAGAAGGG GAAGCTGGTT 
GCAATTGCCC AAGGTGAGGA AGAGAGTACC TCACGGCCCT CTGGGTGGGC TTCGTACCTC 
CACAGCTGGT CAGGACTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939662.1
CDS	39..5102
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRGPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQGEEESTSRPSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_018084173.1
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084173.1

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGGTCC AGCTGTTTCA AATCCAGGAA CGAAGAACTT GAGAGAACGG 
CGAAACACTG ACCTTCCTGT TCTCGATGTG CACACTGTAG CTAGGGAAGA GGGAGAAGGT 
ATGGAAACAA CAGACACAGA GTCTGTCTCT TCAGCCAGCA CCTATGTACA ATCCTTGGAA 
CAACTTCTGA ACTCATCAGA AGCAAAACCT GAACCATCTC AGTGGCAAGC AGAACTGACC 
AAGGATGAAC TGATTCAGAA ACTAAACACA ACAGCGAAGA GTGCTGATCA CTTAAATGAA 
TTACTTCGTG AATCAGAAGC AACCAATGCA ATCCTAATGG AACAAATAAA GCTTCTTAAG 
AATGAAATAA GAAGACTGGA AAGAAATCAA GAGAGAGAAA AGTCTGTGGC TAATTTAGAA 
TACTTGAAGA ATGTTCTACT GCAGTTCATA TTTCTAAAAT CAGGAAGCGA GAAAGAGCGG 
CTGCTCCCAG TAATAGACAC CATGTTGCAG CTCAGCCCTG AAGAGAAGGG GAAGCTGGTT 
GCAATTGCCC AAGGTGAGGA AGAGAGTACC TCACGGCCCT CTGGGTGGGC TTCGTACCTC 
CACAGCTGGT CAGGACTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084173.2 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939662.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.

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CloneID	OMa214689
Clone ID Related Accession (Same CDS sequence)	XM_018084173.1 , XM_018084173.2
Accession Version	XM_018084173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021940813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 19, 2019 this sequence version replaced XM_018084173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGGTCC AGCTGTTTCA AATCCAGGAA CGAAGAACTT GAGAGAACGG 
CGAAACACTG ACCTTCCTGT TCTCGATGTG CACACTGTAG CTAGGGAAGA GGGAGAAGGT 
ATGGAAACAA CAGACACAGA GTCTGTCTCT TCAGCCAGCA CCTATGTACA ATCCTTGGAA 
CAACTTCTGA ACTCATCAGA AGCAAAACCT GAACCATCTC AGTGGCAAGC AGAACTGACC 
AAGGATGAAC TGATTCAGAA ACTAAACACA ACAGCGAAGA GTGCTGATCA CTTAAATGAA 
TTACTTCGTG AATCAGAAGC AACCAATGCA ATCCTAATGG AACAAATAAA GCTTCTTAAG 
AATGAAATAA GAAGACTGGA AAGAAATCAA GAGAGAGAAA AGTCTGTGGC TAATTTAGAA 
TACTTGAAGA ATGTTCTACT GCAGTTCATA TTTCTAAAAT CAGGAAGCGA GAAAGAGCGG 
CTGCTCCCAG TAATAGACAC CATGTTGCAG CTCAGCCCTG AAGAGAAGGG GAAGCTGGTT 
GCAATTGCCC AAGGTGAGGA AGAGAGTACC TCACGGCCCT CTGGGTGGGC TTCGTACCTC 
CACAGCTGGT CAGGACTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939662.1
CDS	32..5095
Translation	MEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRGPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQGEEESTSRPSGWASYLHSWSGLR

Target ORF information:

RefSeq Version	XM_018084173.2
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084173.2

ORF Insert Sequence:

ATGGAGGTTC AAGATGCTGG GCCGGAGATG GTGGCGTCTT CTGGCACACC AGGATCAGGC 
AAATCAAAGC TGGACACATT GCCCAAAGAA GACCTCATTA AGTTTGCAAA AAAGCAGATG 
ATGCTTATGC AGAAAGTGAA GTCAAGATGT ACAGAACTGG AGAAAGAGAT TGAAGAACTC 
AGATCTAAAG CAGCTACTGC AGGAGCTGAT GATATTATTC AGGCTCTCAC AGAAAGACTG 
GACGTTGTAC TTCTGGAAAA AGCTGAAAGT CAGCAGCAGT GCATTGCTCT GAGAAAAGAA 
AATATTCAAA TAAAGCAAGA AGGGGAGGCT GCAGTGGCTA AGACAGAAGA ATTGCAGAAA 
ATACTGGAAC AATCAAGTAT TGACTCTCTG GAAGAAATAA AAGCTTTGAA GTGTGAATTA 
GCAAATGCAC AATGCAAGCA CAATGAGGAT TTAAAAAAGC TGAAAATGGA ATTAGATGAA 
CAAGTGAAAA AACAAATGGA GCTGATGGAA CAGGTTGAAC GTCATAGTGA CAGTCAAAAA 
GAAGTTACAA GATTACAAGA TGATGTCCAG AGAATGAAAT CTACCTATGA GGAGCAAATT 
TTGTGTCTGA GCAAGCAGTT GGAAACTGTG ACCGAAGACA AAACCAAAGA AGTGACAAGT 
CTGCAAGAAA CTATTAAAAA CAACTCTCAA TGTTACCATA ATGAAATAAA AAATCTGAAT 
GAAGAGCTTA AAAAATTAAA AATTGCCCAT CAGGAAGAGG TGTCAGAATT GATGCATCGG 
ATTGAAATAT CATCTAAAGA AAATGAAGAA AAGCAAAATC AAATAAATGA GTTACAGCAC 
AATTTGGCAG AGAAAAATGC AAAGGATGAA ATGCATTGTC ATACTGACCA GCGCGAATAC 
GACTTGGAGC AGCTGAGAGA AGTCTTAAAT AAAAATGTAG AAAACAAGAT AGATATTGCA 
GATACACCAG AAGAACCTTA CATAGAAGCA AAAATGGAAG CAAAGGTTAG GTACCTGGAG 
CATAGTTTAG AAGAACTCCA GTCCCAACAT AGTATATTAA AGGATGAGTT AACTTACATG 
AGCAATGTCA AACTAAAGCT GGAGGAGGAA ATCCATCGCA TAAAGGATGA GTACTTTCAT 
GAGCGGGAAG ATTTGGACTT TAAGATAAAT GAATTGCAGC TTGCTAAAGA AGATTACTGC 
TGTGTAATTG AAAAACTAAA ATTGGAGCTT AAAGCAGCAA GACAGCACTA TGAAGCTGAT 
TTAGAAGAGC ATAAATTAGA GATTCAAACT CTGAAAGAGC AACACAAGAC AGAAATTTCT 
GAATTAAATG AAACTTTATT ATCTAGTTCT GAAAAAGAAA AGATGGTATT AGTTTTTGAA 
ATACAGGAAC TTAGAGAACA ACTTGAAAAG CTAACTCAGG AGAAAGAAGA AGCAGTGTCC 
AATTACAATA GCCTGAGAGA AACAATGGAA ACTCTCCAGG CTGAGCTAGG CGAATCTGCT 
GGAAAGATCA GCCAGGAGTT TGAATCAATG AAACAACAGC AAGCTTCTGA TGTCAGTCAA 
CTGCAACAGA AACTCCGAGC TGCTTTCAAT GAAAGGGATG TTCTTCTTGA AACTGTAAAT 
CGCCTCCAGG AAGAAATAGA AAAATTGCCA TCTAATCAGC TAGAGATAGA AGAACTTAAA 
TGCAAAATTG TTAGTCTTCA GGAGGACAAT AACGCAATAA GAAGCTCCAT CAACCAAAAA 
GAGATTACTG TAAAAGAACT GGAAGAGAAG ATAGTTGCTC TTACTGATCA AAACAAGGAT 
ATTTTAAATG ATGTAAAATG TTTGGGTGAA GAGAGAGAAA CCCTTCAGGA AAGGTGCAAG 
CAAGAACAAG TTAAAATTCA GGAACTTCAG CAAGAAGTAG ATGTTGCTAA CCAGAACAAT 
AATGACCTGA CGAAAAAGGT AGAGGAATTA ACAGAGAGAC TGAATGAAGC TTTAACTACA 
AAGACTGAAA ATGCTCAAAT GCTAGAGCAA CTGGAAAAAC AGATTGAATC TCTGGTACAT 
GATAGAGAGC AGCTTTCATC TGAAGTGTAT ACTCTTCATG AGGAAAATAA GAAAATCATT 
CAGGAAAAAG GTGAATTAAG TGAAGAGCTG GGAAAGATTA CATCTGAAAA AGATGGTTGG 
TTAGTGTTGA AAGAGCAGTC TGAAAACTTA GAAAAGAAAC TACAAACGAT GACTGCAGAA 
AAAGACCATG TATCAACATT ACTCGAAAAT GAACAAAGGC ACACGTCCCT TGTAAGAACT 
CAACTGTACC ACCTAGTTGA GAAAATGGGG TCCGACATTT CAGAGTCTAA TGAAGAATAT 
GATTCTGTTA ACTTGTTAAA AATTGCAAAT GAATGCCTGT CAAAAATTAA AGAAAAGCAG 
TGCCTTGCTC TACAAAATGA GGAGGAAGTA ATTCATTTGC AGCGGGAAGT TGAGAGACTA 
GAGGAAGAAC ATGCTGCTCA ATATAGAGAA CATAGATCCC TGATTCAGGA TTTTGAAAAA 
GAAAAGGCTC TCTTGAGAGA GGAACTGGAA GGAGTGTTGT CTGAAAAGGA AGCTCTTCAG 
CATGATATTC AGGAGCTGAA GAATGCCAGT GAGAAAACAA GGATTGAAAA TCAAGATCTT 
TTAGCTAATA TTGAAGAGAT CACTCAAAAA CTTGCTTTTT ATGAAAGTCC AATGCAAGAA 
CAGGAAAAAG GATCAGAAAA AAAAGACGAC TTAAGTTGCA TTCTGGAACA AAAGGAAACT 
GAACTTAGAA ATGTGAAAGA CGAACTGAGT TCTCTAAAGA ATTTAATGGA GACAATGACT 
GAGAAAACTG ATCAGCAGTC TTCAGTAGCA GAACTTCAAG AAAAAATTGG GAGGCTGGAA 
AAAGAATCTG CAGAAAAAGG AGAGAAGCTA AATAAAATTA AGGTGGTTGC TGTGAAAGCA 
AAAAAGGAAT TAGATGCCAG CCGAAAAGAG ATGCAAGCTC TGAACGAAGA ATTGGAGTTG 
GTTCGATCAG AAAAAGATCA GTTGTCTGCT TCAATGAAAG ACGTTATTCA AGGAGCTGAA 
AGCTATAAGA ATCTTTTGAT GGAATATGAT AAGCAAGGAG AACAGCTGGA TTCTGAAAAA 
GGCAGAGCAA ATAATCTTGA ACGTCAGATA GATGACCTCA CAAGACAGCT ACAGGTGTCA 
TCCCAGCAGC ATGATCAATT ACGCTCTGCT AATGAAGACC TCCTGGCTCG TGTTGAAACA 
CTGCAAAATA ATGCTAAGCT GCTGGAAACT CAGATATTGG AGATGCAAAG AGCCAAGGCA 
AAGGCTGACA AAGAACTAGA AGCTGAAAGG CTTTTAAGAG AACAGAAAAC AAAGGAACAT 
AGTGGTGCTC TCCGAGAAAT GGAGGAGCTT CAGATGCAAC TTCAGAAGGA AAAGAAACAT 
CTGCAGAAAA CTATGTTAGA ACTAGAGCTG GCCAGAAAGG ATGCTCAAAA GAGTACACTA 
ATGGATATGG AAATAGCTGA TTATGAAAGG CTGGTAAAAG AACTTAATCA GAAGATTACT 
GATAAAGACA GTAAAATAGA AGATCTTGAG CAAGAAACGA GTATTCAGAA ACAGAAGCAA 
GAGACCCTAC AGGAAGAAAT AAAATTCCTT CAGTCAACTA TGCAACAGGA TGAGGAAAGA 
AATGCCAAGA TAAAACAACT CCTAGTAAAA ACCAAAAAAG AACTGGCTGA TTCAAAACAA 
GCTGAAAATG ATCATCTAAT GTTGCAGGAA TCCTTGAAAG GGGAATTGGA AGCCACTCAA 
CAGCAAGTGG AAGCCTATAA GATTCAAGTG GCTGTGCTGA CATCAGAAAA GCATAAAGTT 
CAGGAACAGC TGCGAACGTC TTCGGAGCAA CACCAGCGTA TGATGAGTGC TTGCCAGCAA 
AAAATTGCAA CCCTGCAAGA AGAGTGCAGA GCAGCCCAGG CTGAACAAGC ATCTGTTACG 
TCAGAATTTG AGAGCTACAA AGTCCGTGTT CATAATGTTC TAAAACAGCA AAAGAATAAA 
TCTGCTGCTC GGACAGAGTC TGAAGGAGCC AAACAAGAGA GAGAACAGTT GGAAATGGTG 
ATAGATGAAC TTAAAGTTAA GCTGCAAGAT GCTCAGCATA ACTCACAGAT GAATGCATCT 
GAACTCCAGG CACTGCAGTC TGAACACGAC ACCCTGCTGG AAAGACATAA TAAGATGCTG 
CAAGAGACTG TTGCAAAAGA AGCAGAACTC CGTGAAAAAC TCTGCACAAT ACAGTCTGAG 
AACATGGTCA TGAAAACAGA GCACGCCCAG ATGTTGAGTC AGCTGACAGC CCAGAATGAA 
GCTCTTCGGA ACAGTTTCAG AGATCAAGTC AGGAACCTGC AAGAAGAGCA CAGGAAAACA 
GTAGAGACAC TTCAGCAGCA ACTCTCCAGA GTAGAAGCTC AGCTCTTCCA ACTCAAGAGT 
GAACCAAGTA CTAGAGGTCC AGCTGTTTCA AATCCAGGAA CGAAGAACTT GAGAGAACGG 
CGAAACACTG ACCTTCCTGT TCTCGATGTG CACACTGTAG CTAGGGAAGA GGGAGAAGGT 
ATGGAAACAA CAGACACAGA GTCTGTCTCT TCAGCCAGCA CCTATGTACA ATCCTTGGAA 
CAACTTCTGA ACTCATCAGA AGCAAAACCT GAACCATCTC AGTGGCAAGC AGAACTGACC 
AAGGATGAAC TGATTCAGAA ACTAAACACA ACAGCGAAGA GTGCTGATCA CTTAAATGAA 
TTACTTCGTG AATCAGAAGC AACCAATGCA ATCCTAATGG AACAAATAAA GCTTCTTAAG 
AATGAAATAA GAAGACTGGA AAGAAATCAA GAGAGAGAAA AGTCTGTGGC TAATTTAGAA 
TACTTGAAGA ATGTTCTACT GCAGTTCATA TTTCTAAAAT CAGGAAGCGA GAAAGAGCGG 
CTGCTCCCAG TAATAGACAC CATGTTGCAG CTCAGCCCTG AAGAGAAGGG GAAGCTGGTT 
GCAATTGCCC AAGGTGAGGA AGAGAGTACC TCACGGCCCT CTGGGTGGGC TTCGTACCTC 
CACAGCTGGT CAGGACTTCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCC2 ( XM_018084169.1 ) cDNA ORF clone, Manacus vitellinus(golden-collared manakin) -> XP_017939658.1 Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

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CloneID	OMa214685
Clone ID Related Accession (Same CDS sequence)	XM_018084169.1
Accession Version	XM_018084169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-24
Organism	Manacus vitellinus(golden-collared manakin)
Product	GRIP and coiled-coil domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020340930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Manacus vitellinus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CGGAAGCGGG CCGGGGAGGA TGTAGCGCGC TGACAGCGGC CTGCAGCGCC 
GCGACCTGCA CGCCCCTGCC GCCGGCCGGC CCCGCCATGG AGGTTCAAGA TGCTGGGCCG 
GAGATGGTGG CGTCTTCTGG CACACCAGGA TCAGGCAAAT CAAAGCTGGA CACATTGCCC 
AAAGAAGACC TCATTAAGTT TGCAAAAAAG CAGATGATGC TTATGCAGAA AGTGAAGTCA 
AGATGTACAG AACTGGAGAA AGAGATTGAA GAACTCAGAT CTAAAGCAGC TACTGCAGGA 
GCTGATGATA TTATTCAGGC TCTCACAGAA AGACTGGACG TTGTACTTCT GGAAAAAGCT 
GAAAGTCAGC AGCAGTGCAT TGCTCTGAGA AAAGAAAATA TTCAAATAAA GCAAGAAGGG 
GAGGCTGCAG TGGCTAAGAC AGAAGAATTG CAGAAAATAC TGGAACAATC AAGTATTGAC 
TCTCTGGAAG AAATAAAAGC TTTGAAGTGT GAATTAGCAA ATGCACAATG CAAGCACAAT 
GAGGATTTAA AAAAGCTGAA AATGGAATTA GATGAACAAG TGAAAAAACA AATGGAGCTG 
ATGGAACAGG TTGAACGTCA TAGTGACAGT CAAAAAGAAG TTACAAGATT ACAAGATGAT 
GTCCAGAGAA TGAAATCTAC CTATGAGGAG CAAATTTTGT GTCTGAGCAA GCAGTTGGAA 
ACTGTGACCG AAGACAAAAC CAAAGAAGTG ACAAGTCTGC AAGAAACTAT TAAAAACAAC 
TCTCAATGTT ACCATAATGA AATAAAAAAT CTGAATGAAG AGCTTAAAAA ATTAAAAATT 
GCCCATCAGG AAGAGGTGTC AGAATTGATG CATCGGATTG AAATATCATC TAAAGAAAAT 
GAAGAAAAGC AAAATCAAAT AAATGAGTTA CAGCACAATT TGGCAGAGAA AAATGCAAAG 
GATGAAATGC ATTGTCATAC TGACCAGCGC GAATACGACT TGGAGCAGCT GAGAGAAGTC 
TTAAATAAAA ATGTAGAAAA CAAGATAGAT ATTGCAGATA CACCAGAAGA ACCTTACATA 
GAAGCAAAAA TGGAAGCAAA GGTTAGGTAC CTGGAGCATA GTTTAGAAGA ACTCCAGTCC 
CAACATAGTA TATTAAAGGA TGAGTTAACT TACATGAGCA ATGTCAAACT AAAGCTGGAG 
GAGGAAATCC ATCGCATAAA GGATGAGTAC TTTCATGAGC GGGAAGATTT GGACTTTAAG 
ATAAATGAAT TGCAGCTTGC TAAAGAAGAT TACTGCTGTG TAATTGAAAA ACTAAAATTG 
GAGCTTAAAG CAGCAAGACA GCACTATGAA GCTGATTTAG AAGAGCATAA ATTAGAGATT 
CAAACTCTGA AAGAGCAACA CAAGACAGAA ATTTCTGAAT TAAATGAAAC TTTATTATCT 
AGTTCTGAAA AAGAAAAGAT GGTATTAGTT TTTGAAATAC AGGAACTTAG AGAACAACTT 
GAAAAGCTAA CTCAGGAGAA AGAAGAAGCA GTGTCCAATT ACAATAGCCT GAGAGAAACA 
ATGGAAACTC TCCAGGCTGA GCTAGGCGAA TCTGCTGGAA AGATCAGCCA GGAGTTTGAA 
TCAATGAAAC AACAGCAAGC TTCTGATGTC AGTCAACTGC AACAGAAACT CCGAGCTGCT 
TTCAATGAAA GGGATGTTCT TCTTGAAACT GTAAATCGCC TCCAGGAAGA AATAGAAAAA 
TTGCCATCTA ATCAGCTAGA GATAGAAGAA CTTAAATGCA AAATTGTTAG TCTTCAGGAG 
GACAATAACG CAATAAGAAG CTCCATCAAC CAAAAAGAGA TTACTGTAAA AGAACTGGAA 
GAGAAGATAG TTGCTCTTAC TGATCAAAAC AAGGATATTT TAAATGATGT AAAATGTTTG 
GGTGAAGAGA GAGAAACCCT TCAGGAAAGG TGCAAGCAAG AACAAGTTAA AATTCAGGAA 
CTTCAGCAAG AAGTAGATGT TGCTAACCAG AACAATAATG ACCTGACGAA AAAGGTAGAG 
GAATTAACAG AGAGACTGAA TGAAGCTTTA ACTACAAAGA CTGAAAATGC TCAAATGCTA 
GAGCAACTGG AAAAACAGAT TGAATCTCTG GTACATGATA GAGAGCAGCT TTCATCTGAA 
GTGTATACTC TTCATGAGGA AAATAAGAAA ATCATTCAGG AAAAAGGTGA ATTAAGTGAA 
GAGCTGGGAA AGATTACATC TGAAAAAGAT GGTTGGTTAG TGTTGAAAGA GCAGTCTGAA 
AACTTAGAAA AGAAACTACA AACGATGACT GCAGAAAAAG ACCATGTATC AACATTACTC 
GAAAATGAAC AAAGGCACAC GTCCCTTGTA AGAACTCAAC TGTACCACCT AGTTGAGAAA 
ATGGGGTCCG ACATTTCAGA GTCTAATGAA GAATATGATT CTGTTAACTT GTTAAAAATT 
GCAAATGAAT GCCTGTCAAA AATTAAAGAA AAGCAGTGCC TTGCTCTACA AAATGAGGAG 
GAAGTAATTC ATTTGCAGCG GGAAGTTGAG AGACTAGAGG AAGAACATGC TGCTCAATAT 
AGAGAACATA GATCCCTGAT TCAGGATTTT GAAAAAGAAA AGGCTCTCTT GAGAGAGGAA 
CTGGAAGGAG TGTTGTCTGA AAAGGAAGCT CTTCAGCATG ATATTCAGGA GCTGAAGAAT 
GCCAGTGAGA AAACAAGGAT TGAAAATCAA GATCTTTTAG CTAATATTGA AGAGATCACT 
CAAAAACTTG CTTTTTATGA AAGTCCAATG CAAGAACAGG AAAAAGGATC AGAAAAAAAA 
GACGACTTAA GTTGCATTCT GGAACAAAAG GAAACTGAAC TTAGAAATGT GAAAGACGAA 
CTGAGTTCTC TAAAGAATTT AATGGAGACA ATGACTGAGA AAACTGATCA GCAGTCTTCA 
GTAGCAGAAC TTCAAGAAAA AATTGGGAGG CTGGAAAAAG AATCTGCAGA AAAAGGAGAG 
AAGCTAAATA AAATTAAGGT GGTTGCTGTG AAAGCAAAAA AGGAATTAGA TGCCAGCCGA 
AAAGAGATGC AAGCTCTGAA CGAAGAATTG GAGTTGGTTC GATCAGAAAA AGATCAGTTG 
TCTGCTTCAA TGAAAGACGT TATTCAAGGA GCTGAAAGCT ATAAGAATCT TTTGATGGAA 
TATGATAAGC AAGGAGAACA GCTGGATTCT GAAAAAGGCA GAGCAAATAA TCTTGAACGT 
CAGATAGATG ACCTCACAAG ACAGCTACAG GTGTCATCCC AGCAGCATGA TCAATTACGC 
TCTGCTAATG AAGACCTCCT GGCTCGTGTT GAAACACTGC AAAATAATGC TAAGCTGCTG 
GAAACTCAGA TATTGGAGAT GCAAAGAGCC AAGGCAAAGG CTGACAAAGA ACTAGAAGCT 
GAAAGGCTTT TAAGAGAACA GAAAACAAAG GAACATAGTG GTGCTCTCCG AGAAATGGAG 
GAGCTTCAGA TGCAACTTCA GAAGGAAAAG AAACATCTGC AGAAAACTAT GTTAGAACTA 
GAGCTGGCCA GAAAGGATGC TCAAAAGAGT ACACTAATGG ATATGGAAAT AGCTGATTAT 
GAAAGGCTGG TAAAAGAACT TAATCAGAAG ATTACTGATA AAGACAGTAA AATAGAAGAT 
CTTGAGCAAG AAACGAGTAT TCAGAAACAG AAGCAAGAGA CCCTACAGGA AGAAATAAAA 
TTCCTTCAGT CAACTATGCA ACAGGATGAG GAAAGAAATG CCAAGATAAA ACAACTCCTA 
GTAAAAACCA AAAAAGAACT GGCTGATTCA AAACAAGCTG AAAATGATCA TCTAATGTTG 
CAGGAATCCT TGAAAGGGGA ATTGGAAGCC ACTCAACAGC AAGTGGAAGC CTATAAGATT 
CAAGTGGCTG TGCTGACATC AGAAAAGCAT AAAGTTCAGG AACAGCTGCG AACGTCTTCG 
GAGCAACACC AGCGTATGAT GAGTGCTTGC CAGCAAAAAA TTGCAACCCT GCAAGAAGAG 
TGCAGAGCAG CCCAGGCTGA ACAAGCATCT GTTACGTCAG AATTTGAGAG CTACAAAGTC 
CGTGTTCATA ATGTTCTAAA ACAGCAAAAG AATAAATCTG CTGCTCGGAC AGAGTCTGAA 
GGAGCCAAAC AAGAGAGAGA ACAGTTGGAA ATGGTGATAG ATGAACTTAA AGTTAAGCTG 
CAAGATGCTC AGCATAACTC ACAGATGAAT GCATCTGAAC TCCAGGCACT GCAGTCTGAA 
CACGACACCC TGCTGGAAAG ACATAATAAG ATGCTGCAAG AGACTGTTGC AAAAGAAGCA 
GAACTCCGTG AAAAACTCTG CACAATACAG TCTGAGAACA TGGTCATGAA AACAGAGCAC 
GCCCAGATGT TGAGTCAGCT GACAGCCCAG AATGAAGCTC TTCGGAACAG TTTCAGAGAT 
CAAGTCAGGA ACCTGCAAGA AGAGCACAGG AAAACAGTAG AGACACTTCA GCAGCAACTC 
TCCAGAGTAG AAGCTCAGCT CTTCCAACTC AAGAGTGAAC CAAGTACTAG AGGTCCAGCT 
GTTTCAAATC CAGGAACGAA GAACTTGAGA GAACGGCGAA ACACTGACCT TCCTGTTCTC 
GATGTGCACA CTGTAGCTAG GGAAGAGGGA GAAGGTATGG AAACAACAGA CACAGAGTCT 
GTCTCTTCAG CCAGCACCTA TGTACAATCC TTGGAACAAC TTCTGAACTC ATCAGAAGCA 
AAACCTGAAC CATCTCAGTG GCAAGCAGAA CTGACCAAGG ATGAACTGAT TCAGAAACTA 
AACACAACAG CGAAGAGTGC TGATCACTTA AATGAATTAC TTCGTGAATC AGAAGCAACC 
AATGCAATCC TAATGGAACA AATAAAGCTT CTTAAGAATG AAATAAGAAG ACTGGAAAGA 
AATCAAGAGA GAGAAAAGTC TGTGGCTAAT TTAGAATACT TGAAGAATGT TCTACTGCAG 
TTCATATTTC TAAAATCAGG AAGCGAGAAA GAGCGGCTGC TCCCAGTAAT AGACACCATG 
TTGCAGCTCA GCCCTGAAGA GAAGGGGAAG CTGGTTGCAA TTGCCCAAGT GCCGTGTTTG 
TTAGAATGTT TCCTAGAAAA TGTCACCCTT GCCCAAAGAG TTTATAAGCT TAGGACCAAT 
TTTTATCTTC TATCAGCCTG CCTTCAGCCC AAGCCTAAGG AGGGCATAAT AATAATAATA 
ATAATAATAA AGATTCTGCT GTCACTATGC AGAGTATATT TATTAATTAA AAACCAAAAA 
TGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017939658.1
CDS	1..5289
Translation	MTEAEAGRGGCSALTAACSAATCTPLPPAGPAMEVQDAGPEMVASSGTPGSGKSKLDTLPKEDLIKFAKKQMMLMQKVKSRCTELEKEIEELRSKAATAGADDIIQALTERLDVVLLEKAESQQQCIALRKENIQIKQEGEAAVAKTEELQKILEQSSIDSLEEIKALKCELANAQCKHNEDLKKLKMELDEQVKKQMELMEQVERHSDSQKEVTRLQDDVQRMKSTYEEQILCLSKQLETVTEDKTKEVTSLQETIKNNSQCYHNEIKNLNEELKKLKIAHQEEVSELMHRIEISSKENEEKQNQINELQHNLAEKNAKDEMHCHTDQREYDLEQLREVLNKNVENKIDIADTPEEPYIEAKMEAKVRYLEHSLEELQSQHSILKDELTYMSNVKLKLEEEIHRIKDEYFHEREDLDFKINELQLAKEDYCCVIEKLKLELKAARQHYEADLEEHKLEIQTLKEQHKTEISELNETLLSSSEKEKMVLVFEIQELREQLEKLTQEKEEAVSNYNSLRETMETLQAELGESAGKISQEFESMKQQQASDVSQLQQKLRAAFNERDVLLETVNRLQEEIEKLPSNQLEIEELKCKIVSLQEDNNAIRSSINQKEITVKELEEKIVALTDQNKDILNDVKCLGEERETLQERCKQEQVKIQELQQEVDVANQNNNDLTKKVEELTERLNEALTTKTENAQMLEQLEKQIESLVHDREQLSSEVYTLHEENKKIIQEKGELSEELGKITSEKDGWLVLKEQSENLEKKLQTMTAEKDHVSTLLENEQRHTSLVRTQLYHLVEKMGSDISESNEEYDSVNLLKIANECLSKIKEKQCLALQNEEEVIHLQREVERLEEEHAAQYREHRSLIQDFEKEKALLREELEGVLSEKEALQHDIQELKNASEKTRIENQDLLANIEEITQKLAFYESPMQEQEKGSEKKDDLSCILEQKETELRNVKDELSSLKNLMETMTEKTDQQSSVAELQEKIGRLEKESAEKGEKLNKIKVVAVKAKKELDASRKEMQALNEELELVRSEKDQLSASMKDVIQGAESYKNLLMEYDKQGEQLDSEKGRANNLERQIDDLTRQLQVSSQQHDQLRSANEDLLARVETLQNNAKLLETQILEMQRAKAKADKELEAERLLREQKTKEHSGALREMEELQMQLQKEKKHLQKTMLELELARKDAQKSTLMDMEIADYERLVKELNQKITDKDSKIEDLEQETSIQKQKQETLQEEIKFLQSTMQQDEERNAKIKQLLVKTKKELADSKQAENDHLMLQESLKGELEATQQQVEAYKIQVAVLTSEKHKVQEQLRTSSEQHQRMMSACQQKIATLQEECRAAQAEQASVTSEFESYKVRVHNVLKQQKNKSAARTESEGAKQEREQLEMVIDELKVKLQDAQHNSQMNASELQALQSEHDTLLERHNKMLQETVAKEAELREKLCTIQSENMVMKTEHAQMLSQLTAQNEALRNSFRDQVRNLQEEHRKTVETLQQQLSRVEAQLFQLKSEPSTRGPAVSNPGTKNLRERRNTDLPVLDVHTVAREEGEGMETTDTESVSSASTYVQSLEQLLNSSEAKPEPSQWQAELTKDELIQKLNTTAKSADHLNELLRESEATNAILMEQIKLLKNEIRRLERNQEREKSVANLEYLKNVLLQFIFLKSGSEKERLLPVIDTMLQLSPEEKGKLVAIAQVPCLLECFLENVTLAQRVYKLRTNFYLLSACLQPKPKEGIIIIIIIIKILLSLCRVYLLIKNQKWE

Target ORF information:

RefSeq Version	XM_018084169.1
Organism	Manacus vitellinus(golden-collared manakin)
Definition	Manacus vitellinus GRIP and coiled-coil domain containing 2 (GCC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018084169.1

ORF Insert Sequence:

ATGACGGAGG CGGAAGCGGG CCGGGGAGGA TGTAGCGCGC TGACAGCGGC CTGCAGCGCC 
GCGACCTGCA CGCCCCTGCC GCCGGCCGGC CCCGCCATGG AGGTTCAAGA TGCTGGGCCG 
GAGATGGTGG CGTCTTCTGG CACACCAGGA TCAGGCAAAT CAAAGCTGGA CACATTGCCC 
AAAGAAGACC TCATTAAGTT TGCAAAAAAG CAGATGATGC TTATGCAGAA AGTGAAGTCA 
AGATGTACAG AACTGGAGAA AGAGATTGAA GAACTCAGAT CTAAAGCAGC TACTGCAGGA 
GCTGATGATA TTATTCAGGC TCTCACAGAA AGACTGGACG TTGTACTTCT GGAAAAAGCT 
GAAAGTCAGC AGCAGTGCAT TGCTCTGAGA AAAGAAAATA TTCAAATAAA GCAAGAAGGG 
GAGGCTGCAG TGGCTAAGAC AGAAGAATTG CAGAAAATAC TGGAACAATC AAGTATTGAC 
TCTCTGGAAG AAATAAAAGC TTTGAAGTGT GAATTAGCAA ATGCACAATG CAAGCACAAT 
GAGGATTTAA AAAAGCTGAA AATGGAATTA GATGAACAAG TGAAAAAACA AATGGAGCTG 
ATGGAACAGG TTGAACGTCA TAGTGACAGT CAAAAAGAAG TTACAAGATT ACAAGATGAT 
GTCCAGAGAA TGAAATCTAC CTATGAGGAG CAAATTTTGT GTCTGAGCAA GCAGTTGGAA 
ACTGTGACCG AAGACAAAAC CAAAGAAGTG ACAAGTCTGC AAGAAACTAT TAAAAACAAC 
TCTCAATGTT ACCATAATGA AATAAAAAAT CTGAATGAAG AGCTTAAAAA ATTAAAAATT 
GCCCATCAGG AAGAGGTGTC AGAATTGATG CATCGGATTG AAATATCATC TAAAGAAAAT 
GAAGAAAAGC AAAATCAAAT AAATGAGTTA CAGCACAATT TGGCAGAGAA AAATGCAAAG 
GATGAAATGC ATTGTCATAC TGACCAGCGC GAATACGACT TGGAGCAGCT GAGAGAAGTC 
TTAAATAAAA ATGTAGAAAA CAAGATAGAT ATTGCAGATA CACCAGAAGA ACCTTACATA 
GAAGCAAAAA TGGAAGCAAA GGTTAGGTAC CTGGAGCATA GTTTAGAAGA ACTCCAGTCC 
CAACATAGTA TATTAAAGGA TGAGTTAACT TACATGAGCA ATGTCAAACT AAAGCTGGAG 
GAGGAAATCC ATCGCATAAA GGATGAGTAC TTTCATGAGC GGGAAGATTT GGACTTTAAG 
ATAAATGAAT TGCAGCTTGC TAAAGAAGAT TACTGCTGTG TAATTGAAAA ACTAAAATTG 
GAGCTTAAAG CAGCAAGACA GCACTATGAA GCTGATTTAG AAGAGCATAA ATTAGAGATT 
CAAACTCTGA AAGAGCAACA CAAGACAGAA ATTTCTGAAT TAAATGAAAC TTTATTATCT 
AGTTCTGAAA AAGAAAAGAT GGTATTAGTT TTTGAAATAC AGGAACTTAG AGAACAACTT 
GAAAAGCTAA CTCAGGAGAA AGAAGAAGCA GTGTCCAATT ACAATAGCCT GAGAGAAACA 
ATGGAAACTC TCCAGGCTGA GCTAGGCGAA TCTGCTGGAA AGATCAGCCA GGAGTTTGAA 
TCAATGAAAC AACAGCAAGC TTCTGATGTC AGTCAACTGC AACAGAAACT CCGAGCTGCT 
TTCAATGAAA GGGATGTTCT TCTTGAAACT GTAAATCGCC TCCAGGAAGA AATAGAAAAA 
TTGCCATCTA ATCAGCTAGA GATAGAAGAA CTTAAATGCA AAATTGTTAG TCTTCAGGAG 
GACAATAACG CAATAAGAAG CTCCATCAAC CAAAAAGAGA TTACTGTAAA AGAACTGGAA 
GAGAAGATAG TTGCTCTTAC TGATCAAAAC AAGGATATTT TAAATGATGT AAAATGTTTG 
GGTGAAGAGA GAGAAACCCT TCAGGAAAGG TGCAAGCAAG AACAAGTTAA AATTCAGGAA 
CTTCAGCAAG AAGTAGATGT TGCTAACCAG AACAATAATG ACCTGACGAA AAAGGTAGAG 
GAATTAACAG AGAGACTGAA TGAAGCTTTA ACTACAAAGA CTGAAAATGC TCAAATGCTA 
GAGCAACTGG AAAAACAGAT TGAATCTCTG GTACATGATA GAGAGCAGCT TTCATCTGAA 
GTGTATACTC TTCATGAGGA AAATAAGAAA ATCATTCAGG AAAAAGGTGA ATTAAGTGAA 
GAGCTGGGAA AGATTACATC TGAAAAAGAT GGTTGGTTAG TGTTGAAAGA GCAGTCTGAA 
AACTTAGAAA AGAAACTACA AACGATGACT GCAGAAAAAG ACCATGTATC AACATTACTC 
GAAAATGAAC AAAGGCACAC GTCCCTTGTA AGAACTCAAC TGTACCACCT AGTTGAGAAA 
ATGGGGTCCG ACATTTCAGA GTCTAATGAA GAATATGATT CTGTTAACTT GTTAAAAATT 
GCAAATGAAT GCCTGTCAAA AATTAAAGAA AAGCAGTGCC TTGCTCTACA AAATGAGGAG 
GAAGTAATTC ATTTGCAGCG GGAAGTTGAG AGACTAGAGG AAGAACATGC TGCTCAATAT 
AGAGAACATA GATCCCTGAT TCAGGATTTT GAAAAAGAAA AGGCTCTCTT GAGAGAGGAA 
CTGGAAGGAG TGTTGTCTGA AAAGGAAGCT CTTCAGCATG ATATTCAGGA GCTGAAGAAT 
GCCAGTGAGA AAACAAGGAT TGAAAATCAA GATCTTTTAG CTAATATTGA AGAGATCACT 
CAAAAACTTG CTTTTTATGA AAGTCCAATG CAAGAACAGG AAAAAGGATC AGAAAAAAAA 
GACGACTTAA GTTGCATTCT GGAACAAAAG GAAACTGAAC TTAGAAATGT GAAAGACGAA 
CTGAGTTCTC TAAAGAATTT AATGGAGACA ATGACTGAGA AAACTGATCA GCAGTCTTCA 
GTAGCAGAAC TTCAAGAAAA AATTGGGAGG CTGGAAAAAG AATCTGCAGA AAAAGGAGAG 
AAGCTAAATA AAATTAAGGT GGTTGCTGTG AAAGCAAAAA AGGAATTAGA TGCCAGCCGA 
AAAGAGATGC AAGCTCTGAA CGAAGAATTG GAGTTGGTTC GATCAGAAAA AGATCAGTTG 
TCTGCTTCAA TGAAAGACGT TATTCAAGGA GCTGAAAGCT ATAAGAATCT TTTGATGGAA 
TATGATAAGC AAGGAGAACA GCTGGATTCT GAAAAAGGCA GAGCAAATAA TCTTGAACGT 
CAGATAGATG ACCTCACAAG ACAGCTACAG GTGTCATCCC AGCAGCATGA TCAATTACGC 
TCTGCTAATG AAGACCTCCT GGCTCGTGTT GAAACACTGC AAAATAATGC TAAGCTGCTG 
GAAACTCAGA TATTGGAGAT GCAAAGAGCC AAGGCAAAGG CTGACAAAGA ACTAGAAGCT 
GAAAGGCTTT TAAGAGAACA GAAAACAAAG GAACATAGTG GTGCTCTCCG AGAAATGGAG 
GAGCTTCAGA TGCAACTTCA GAAGGAAAAG AAACATCTGC AGAAAACTAT GTTAGAACTA 
GAGCTGGCCA GAAAGGATGC TCAAAAGAGT ACACTAATGG ATATGGAAAT AGCTGATTAT 
GAAAGGCTGG TAAAAGAACT TAATCAGAAG ATTACTGATA AAGACAGTAA AATAGAAGAT 
CTTGAGCAAG AAACGAGTAT TCAGAAACAG AAGCAAGAGA CCCTACAGGA AGAAATAAAA 
TTCCTTCAGT CAACTATGCA ACAGGATGAG GAAAGAAATG CCAAGATAAA ACAACTCCTA 
GTAAAAACCA AAAAAGAACT GGCTGATTCA AAACAAGCTG AAAATGATCA TCTAATGTTG 
CAGGAATCCT TGAAAGGGGA ATTGGAAGCC ACTCAACAGC AAGTGGAAGC CTATAAGATT 
CAAGTGGCTG TGCTGACATC AGAAAAGCAT AAAGTTCAGG AACAGCTGCG AACGTCTTCG 
GAGCAACACC AGCGTATGAT GAGTGCTTGC CAGCAAAAAA TTGCAACCCT GCAAGAAGAG 
TGCAGAGCAG CCCAGGCTGA ACAAGCATCT GTTACGTCAG AATTTGAGAG CTACAAAGTC 
CGTGTTCATA ATGTTCTAAA ACAGCAAAAG AATAAATCTG CTGCTCGGAC AGAGTCTGAA 
GGAGCCAAAC AAGAGAGAGA ACAGTTGGAA ATGGTGATAG ATGAACTTAA AGTTAAGCTG 
CAAGATGCTC AGCATAACTC ACAGATGAAT GCATCTGAAC TCCAGGCACT GCAGTCTGAA 
CACGACACCC TGCTGGAAAG ACATAATAAG ATGCTGCAAG AGACTGTTGC AAAAGAAGCA 
GAACTCCGTG AAAAACTCTG CACAATACAG TCTGAGAACA TGGTCATGAA AACAGAGCAC 
GCCCAGATGT TGAGTCAGCT GACAGCCCAG AATGAAGCTC TTCGGAACAG TTTCAGAGAT 
CAAGTCAGGA ACCTGCAAGA AGAGCACAGG AAAACAGTAG AGACACTTCA GCAGCAACTC 
TCCAGAGTAG AAGCTCAGCT CTTCCAACTC AAGAGTGAAC CAAGTACTAG AGGTCCAGCT 
GTTTCAAATC CAGGAACGAA GAACTTGAGA GAACGGCGAA ACACTGACCT TCCTGTTCTC 
GATGTGCACA CTGTAGCTAG GGAAGAGGGA GAAGGTATGG AAACAACAGA CACAGAGTCT 
GTCTCTTCAG CCAGCACCTA TGTACAATCC TTGGAACAAC TTCTGAACTC ATCAGAAGCA 
AAACCTGAAC CATCTCAGTG GCAAGCAGAA CTGACCAAGG ATGAACTGAT TCAGAAACTA 
AACACAACAG CGAAGAGTGC TGATCACTTA AATGAATTAC TTCGTGAATC AGAAGCAACC 
AATGCAATCC TAATGGAACA AATAAAGCTT CTTAAGAATG AAATAAGAAG ACTGGAAAGA 
AATCAAGAGA GAGAAAAGTC TGTGGCTAAT TTAGAATACT TGAAGAATGT TCTACTGCAG 
TTCATATTTC TAAAATCAGG AAGCGAGAAA GAGCGGCTGC TCCCAGTAAT AGACACCATG 
TTGCAGCTCA GCCCTGAAGA GAAGGGGAAG CTGGTTGCAA TTGCCCAAGT GCCGTGTTTG 
TTAGAATGTT TCCTAGAAAA TGTCACCCTT GCCCAAAGAG TTTATAAGCT TAGGACCAAT 
TTTTATCTTC TATCAGCCTG CCTTCAGCCC AAGCCTAAGG AGGGCATAAT AATAATAATA 
ATAATAATAA AGATTCTGCT GTCACTATGC AGAGTATATT TATTAATTAA AAACCAAAAA 
TGGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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GCC2 cDNA ORF clone, Manacus vitellinus(golden-collared manakin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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