MID2 cDNA ORF clone, Leptonychotes weddellii(Weddell seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID2
Entrez Gene ID	102748333
Full Name	midline 2
Gene Type	protein-coding
Organism	Leptonychotes weddellii(Weddell seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006751004.2	XP_006751067.2	probable E3 ubiquitin-protein ligase MID2
XM_006751003.1	XP_006751066.1	probable E3 ubiquitin-protein ligase MID2-like isoform X1
XM_006751004.1	XP_006751067.1	probable E3 ubiquitin-protein ligase MID2-like isoform X2

Pathways from BioSystems
Gene Ontology

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe118241	XM_006751003.1 Latest version!	Leptonychotes weddellii midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OLe118242	XM_006751004.1 Latest version!	Leptonychotes weddellii midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID2 ( XM_006751003.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006751066.1 Leptonychotes weddellii midline 2 (MID2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe118241
Clone ID Related Accession (Same CDS sequence)	XM_006751003.1
Accession Version	XM_006751003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	probable E3 ubiquitin-protein ligase MID2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006387343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAACTA 
ATACAGATCT GCCAGCAAGT TGAGGTGAAT ACTGCTATGC ATGAGGCAAA ACTCATGGAA 
GAATGTGACG AGTTGGTAGA GATCATCCAG CAGAGGAAAC AAATGATCGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAG TTGGCACAGC AGGTGGCTAA TTGCCGCCAG 
TGTCTTGAGC GGTCAACGGT CCTCATCAAC CAGGCTGAAC ATATTCTGAA GGAAAATGAC 
CAGGCCCGGT TTCTCCAGTC TGCAAAAAAT ATTGCCGAGA GGGTCGCTAT GGCAACAGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ATTGCTGGAG GGGCTAGATT ATTTAACAGC CCCAAACCCA 
CCATCTATCC GAGAGGAGCT CTGTACGGCC TCCCATGACA CCATTACAGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA CAATTCAGTG 
GATAGCTGGA TGATTGTGCC CAACATTAAA CAGAACCACT ACACAGTGCA TGGACTTCAG 
AGCGGGACCC GCTACATTTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCCACCC GACTAAAAAC AAATAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATCTCCAA TGATGGGCTG CAGATGGAGA AGGATGAGAG CTCTCTGAAG 
AAGAGCCATA CCCCGGAGAG GTTTAGTAGT ACGGGCTGCT ACGGAGCAGC GGGAAATATA 
TTCATTGACA GTGGCTGCCA CTACTGGGAG GTGGTCATGG GTTCCTCCAC CTGGTATGCA 
ATTGGTATTG CCTACAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA TGCCTCCTCG 
TGGGTCTTCT CTCGATGCAA CAGTAACTTT GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGATGTGC CCCCACAGTT GAAACGTCTC GGTGTTCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGACGT GACCTTCATT 
CTTCCAGTTT GCCCAACATT CACAATCTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTATCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006751066.1
CDS	9..1484
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006751003.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006751003.1

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAACTA 
ATACAGATCT GCCAGCAAGT TGAGGTGAAT ACTGCTATGC ATGAGGCAAA ACTCATGGAA 
GAATGTGACG AGTTGGTAGA GATCATCCAG CAGAGGAAAC AAATGATCGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAG TTGGCACAGC AGGTGGCTAA TTGCCGCCAG 
TGTCTTGAGC GGTCAACGGT CCTCATCAAC CAGGCTGAAC ATATTCTGAA GGAAAATGAC 
CAGGCCCGGT TTCTCCAGTC TGCAAAAAAT ATTGCCGAGA GGGTCGCTAT GGCAACAGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ATTGCTGGAG GGGCTAGATT ATTTAACAGC CCCAAACCCA 
CCATCTATCC GAGAGGAGCT CTGTACGGCC TCCCATGACA CCATTACAGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGTAAGTC ATGGTGTAGT TGGGGCCTGT GGCCAGAGAT AAGGAAATGT 
AAGGAAGCAG TAAGCTGCTC AAGATTGGCC GGGGCGCCAC GAGGCCTGTA CAATTCAGTG 
GATAGCTGGA TGATTGTGCC CAACATTAAA CAGAACCACT ACACAGTGCA TGGACTTCAG 
AGCGGGACCC GCTACATTTT CATTGTTAAA GCCATAAACC AAGCAGGCAG CCGGAACAGT 
GAACCCACCC GACTAAAAAC AAATAGCCAA CCCTTTAAAC TGGATCCCAA AATGACTCAC 
AAGAAGTTGA AGATCTCCAA TGATGGGCTG CAGATGGAGA AGGATGAGAG CTCTCTGAAG 
AAGAGCCATA CCCCGGAGAG GTTTAGTAGT ACGGGCTGCT ACGGAGCAGC GGGAAATATA 
TTCATTGACA GTGGCTGCCA CTACTGGGAG GTGGTCATGG GTTCCTCCAC CTGGTATGCA 
ATTGGTATTG CCTACAAATC AGCTCCCAAG AATGAATGGA TTGGCAAGAA TGCCTCCTCG 
TGGGTCTTCT CTCGATGCAA CAGTAACTTT GTAGTGAGAC ATAACAACAA GGAAATGCTG 
GTGGATGTGC CCCCACAGTT GAAACGTCTC GGTGTTCTTC TGGATTATGA CAACAACATG 
CTGTCTTTCT ATGACCCAGC TAACTCTCTC CATCTTCATA CTTTTGACGT GACCTTCATT 
CTTCCAGTTT GCCCAACATT CACAATCTGG AACAAATCCC TAATGATCCT GTCTGGCTTG 
CCTGCCCCAG ATTTTATTGA TTATCCTGAG CGGCAGGAAT GCAACTGCAG GCCTCAAGAA 
TCCCCTTATG TTTCTGGGAT GAAAGCTTGC CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_006751004.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006751067.1 Leptonychotes weddellii midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLe118242
Clone ID Related Accession (Same CDS sequence)	XM_006751004.1
Accession Version	XM_006751004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	probable E3 ubiquitin-protein ligase MID2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006387343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAACTA 
ATACAGATCT GCCAGCAAGT TGAGGTGAAT ACTGCTATGC ATGAGGCAAA ACTCATGGAA 
GAATGTGACG AGTTGGTAGA GATCATCCAG CAGAGGAAAC AAATGATCGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAG TTGGCACAGC AGGTGGCTAA TTGCCGCCAG 
TGTCTTGAGC GGTCAACGGT CCTCATCAAC CAGGCTGAAC ATATTCTGAA GGAAAATGAC 
CAGGCCCGGT TTCTCCAGTC TGCAAAAAAT ATTGCCGAGA GGGTCGCTAT GGCAACAGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ATTGCTGGAG GGGCTAGATT ATTTAACAGC CCCAAACCCA 
CCATCTATCC GAGAGGAGCT CTGTACGGCC TCCCATGACA CCATTACAGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA CAATTCAGTG GATAGCTGGA TGATTGTGCC CAACATTAAA 
CAGAACCACT ACACAGTGCA TGGACTTCAG AGCGGGACCC GCTACATTTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCCACCC GACTAAAAAC AAATAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATCTCCAA TGATGGGCTG 
CAGATGGAGA AGGATGAGAG CTCTCTGAAG AAGAGCCATA CCCCGGAGAG GTTTAGTAGT 
ACGGGCTGCT ACGGAGCAGC GGGAAATATA TTCATTGACA GTGGCTGCCA CTACTGGGAG 
GTGGTCATGG GTTCCTCCAC CTGGTATGCA ATTGGTATTG CCTACAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA TGCCTCCTCG TGGGTCTTCT CTCGATGCAA CAGTAACTTT 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGATGTGC CCCCACAGTT GAAACGTCTC 
GGTGTTCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGACGT GACCTTCATT CTTCCAGTTT GCCCAACATT CACAATCTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTATCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006751067.1
CDS	9..1394
Translation	MNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSSTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006751004.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006751004.1

ORF Insert Sequence:

ATGAACCTCA CCAACCTGGT TAAGCGCAAC AGTGAACTAG AAAATCAAAT GGCCAAACTA 
ATACAGATCT GCCAGCAAGT TGAGGTGAAT ACTGCTATGC ATGAGGCAAA ACTCATGGAA 
GAATGTGACG AGTTGGTAGA GATCATCCAG CAGAGGAAAC AAATGATCGC TGTCAAAATC 
AAAGAGACAA AGGTTATGAA ACTGAGAAAG TTGGCACAGC AGGTGGCTAA TTGCCGCCAG 
TGTCTTGAGC GGTCAACGGT CCTCATCAAC CAGGCTGAAC ATATTCTGAA GGAAAATGAC 
CAGGCCCGGT TTCTCCAGTC TGCAAAAAAT ATTGCCGAGA GGGTCGCTAT GGCAACAGCA 
TCTTCTCAAG TTCTGATTCC AGACATCAAT TTTAATGATG CCTTTGAAAA CTTTGCTTTA 
GATTTTTCCA GAGAAAAGAA ATTGCTGGAG GGGCTAGATT ATTTAACAGC CCCAAACCCA 
CCATCTATCC GAGAGGAGCT CTGTACGGCC TCCCATGACA CCATTACAGT CCACTGGATC 
TCGGATGATG AGTTCAGCAT CAGCTCCTAT GAGCTTCAGT ACACCATATT CACTGGCCAG 
GCTAACTTCA TCAGCCTGTA CAATTCAGTG GATAGCTGGA TGATTGTGCC CAACATTAAA 
CAGAACCACT ACACAGTGCA TGGACTTCAG AGCGGGACCC GCTACATTTT CATTGTTAAA 
GCCATAAACC AAGCAGGCAG CCGGAACAGT GAACCCACCC GACTAAAAAC AAATAGCCAA 
CCCTTTAAAC TGGATCCCAA AATGACTCAC AAGAAGTTGA AGATCTCCAA TGATGGGCTG 
CAGATGGAGA AGGATGAGAG CTCTCTGAAG AAGAGCCATA CCCCGGAGAG GTTTAGTAGT 
ACGGGCTGCT ACGGAGCAGC GGGAAATATA TTCATTGACA GTGGCTGCCA CTACTGGGAG 
GTGGTCATGG GTTCCTCCAC CTGGTATGCA ATTGGTATTG CCTACAAATC AGCTCCCAAG 
AATGAATGGA TTGGCAAGAA TGCCTCCTCG TGGGTCTTCT CTCGATGCAA CAGTAACTTT 
GTAGTGAGAC ATAACAACAA GGAAATGCTG GTGGATGTGC CCCCACAGTT GAAACGTCTC 
GGTGTTCTTC TGGATTATGA CAACAACATG CTGTCTTTCT ATGACCCAGC TAACTCTCTC 
CATCTTCATA CTTTTGACGT GACCTTCATT CTTCCAGTTT GCCCAACATT CACAATCTGG 
AACAAATCCC TAATGATCCT GTCTGGCTTG CCTGCCCCAG ATTTTATTGA TTATCCTGAG 
CGGCAGGAAT GCAACTGCAG GCCTCAAGAA TCCCCTTATG TTTCTGGGAT GAAAGCTTGC 
CATTAA