PYGB cDNA ORF clone, Leptonychotes weddellii(Weddell seal)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGB
Entrez Gene ID	102746309
Full Name	glycogen phosphorylase B
Gene Type	protein-coding
Organism	Leptonychotes weddellii(Weddell seal)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006745448.2	XP_006745511.1	glycogen phosphorylase, brain form
XM_006745446.1	XP_006745509.1	glycogen phosphorylase, brain form isoform X1
XM_006745447.1	XP_006745510.1	glycogen phosphorylase, brain form isoform X2
XM_006745448.1	XP_006745511.1	glycogen phosphorylase, brain form isoform X3

Pathways from BioSystems
Gene Ontology

The following PYGB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLe113503	XM_006745446.1 Latest version!	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OLe113504	XM_006745447.1 Latest version!	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OLe113505	XM_006745448.2 Latest version!	Leptonychotes weddellii glycogen phosphorylase B (PYGB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OLe113505	XM_006745448.1	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PYGB ( XM_006745446.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006745509.1 Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe113503
Clone ID Related Accession (Same CDS sequence)	XM_006745446.1
Accession Version	XM_006745446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	glycogen phosphorylase, brain form isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006385059.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGTTGGGGT TGGACTTGGA AGAACTGGAG GAAATAGAAG AGGACGCTGG CCTCGGGAAC 
GGAGGCTTGG GGAGGCTGGC AGCGTGCTTC CTTGACTCGA TGGCCACGTT GGGCCTGGCA 
GCATATGGCT ATGGAATCCG CTATGAGTTT GGGATTTTTA ACCAGAAGAT TGTCGATGGC 
TGGCAGGTGG AGGAGGCCGA TGACTGGCTG CGCTACGGCA ACCCCTGGGA GAAGGCCCGG 
CCGGAGTACA TGCTGCCTGT GCACTTCTAC GGGCGGGTGG AGCACTGCCT GGAGGGCGTC 
AAGTGGCTGG ACACCCAGGT CGTGCTGGCC ATGCCCTACG ACACCCCGGT GCCCGGCTAC 
AGGAACAACA CCGTCAACAC TATGAGGCTA TGGTCCGCCA AGGCACCCAA CGACTTCAAG 
CTCCATGACT TTAATGTGGG TGGCTACATC GAGGCAGTCC TGGACAGGAA CCTGGCCGAG 
AACATCTCCA GAGTCCTGTA TCCAAATGAT AATTTCTTCG AGGGGAAGGA GCTCCGCCTG 
AAGCAGGAGT ACTTTGTGGT GGCTGCCACC CTCCAGGACA TCATTCGACG CTTTAAGTCC 
TCCAAGTTCG GCTGCCGGGA CCCAGTGAGA ACCTGTTTTG AGACGTTCCC AGACAAGGTT 
GCCATCCAGC TGAACGACAC CCACCCAGCC CTTGCCATCC CTGAGCTCAT GCGGATACTG 
GTGGACGTGG AGAAAGTGGA CTGGGACAAG GCCTGGGAAA TCACAAAGAA GACCTGTGCA 
TACACCAACC ACACCGTGTT ACCCGAGGCC TTAGAGCGCT GGCCCGTGTC CATGTTTGAG 
AAGCTCCTGC CACGGCACCT GGAGATCATC TACGCCATCA ACCAGAGGCA CCTGGATCAC 
GTGGCCGCCC TGTTCCCTGG GGACGTTGAC CGCCTGCGGA GGATGTCTGT GATCGAGGAA 
GGGGACTGCA AGCGGATTAA CATGGCCCAC CTGTGTGTGA TCGGGTCCCA TGCTGTCAAC 
GGCGTGGCGA GGATCCACTC GGAGATTGTG AAGCAGTCAG TCTTTAAGGA CTTTTATGAG 
CTGGAGCCAG AGAAGTTCCA GAATAAGACA AATGGCATCA CACCCCGACG GTGGCTGCTG 
CTGTGTAACC CGGGGCTGGC CGGCACCATC GCGGAGAAAA TTGGGGAGGG TTTCCTGACT 
GACCTGAGCC AACTGAAGAA GCTGTTGCCA TTGGTTGATG ACGAGGCACT CATCAGGGAT 
GTGGCCAAGG TCAAGCAGGA AAACAAGCTG AAGTTCTCTG CCTTGCTGGA GAAGGAATAT 
AAGGTGAAAA TCAACCCTTC CTCCATGTTC GACGTGCACG TGAAGAGGAT CCATGAGTAC 
AAGCGGCAGC TGCTGAACTG CCTGCATATC GTCACCCTGT ACAACCGAAT AAAGAAGGAC 
CCAGCCAGAG CCTTTGTGCC CAGGACTGTC ATGATCGGGG GCAAGGCAGC CCCCGGTTAC 
CACATGGCGA AGATGATCAT CAAGCTGGTC ACATCCATCG GCAGTGTTGT CAACCACGAT 
CCAGTTGTGG GCGACAAGCT CAAAGTGATC TTCCTGGAAA ACTACCGAGT GTCTTTGGCT 
GAGAAAGTGA TCCCCGCAGC AGACCTTTCC CAGCAGATCT CCACTGCGGG CACCGAGGCC 
TCGGGCACGG GCAACATGAA GTTCATGCTC AACGGGGCCC TCACCATTGG TACCATGGAT 
GGAGCCAACG TGGAGATGGC CGAGGAAGCC GGGGCTGAGA ACCTCTTCAT CTTTGGCATG 
CGTGTGGAGG ACGTCGAGGC CCTGGACCAG AAAGGGTACA ATGCCAGAGA ATACTATGAC 
CACCTGCCCG AGCTGAAGCA GGCCGTGGAC CAGATCAGCA GCGGCTTCTT CTCTCCCAAG 
GAACCAGACT GCTTCAGGGA CATGGTCAAC ATGCTGCTGA ACCACGACAG GTTCAAGGTG 
TTTGCAGATT ATGAAGCGTA CGTGGCATGC CAGGCCCAGG TGGACCAGCT GTACCGGAAC 
CCCAGGGAGT GGACCAAGAA GGTCATCAGG AACATCGCCT GCTCGGGCAA GTTCTCCAGT 
GACCGGACCA TCACAGAGTA CGCCCGGGAC ATCTGGGGCG TGGAGCCCTC CGACCTGCAG 
ATCCCGCCCC CCAACCTCCC CAGGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006745509.1
CDS	1..2430
Translation	MAKPLTDSEKRKQISVRGIAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVDGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHCLEGVKWLDTQVVLAMPYDTPVPGYRNNTVNTMRLWSAKAPNDFKLHDFNVGGYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALAIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLAGTIAEKIGEGFLTDLSQLKKLLPLVDDEALIRDVAKVKQENKLKFSALLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHIVTLYNRIKKDPARAFVPRTVMIGGKAAPGYHMAKMIIKLVTSIGSVVNHDPVVGDKLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGMRVEDVEALDQKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFRDMVNMLLNHDRFKVFADYEAYVACQAQVDQLYRNPREWTKKVIRNIACSGKFSSDRTITEYARDIWGVEPSDLQIPPPNLPRD

Target ORF information:

RefSeq Version	XM_006745446.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006745446.1

ORF Insert Sequence:

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGTTGGGGT TGGACTTGGA AGAACTGGAG GAAATAGAAG AGGACGCTGG CCTCGGGAAC 
GGAGGCTTGG GGAGGCTGGC AGCGTGCTTC CTTGACTCGA TGGCCACGTT GGGCCTGGCA 
GCATATGGCT ATGGAATCCG CTATGAGTTT GGGATTTTTA ACCAGAAGAT TGTCGATGGC 
TGGCAGGTGG AGGAGGCCGA TGACTGGCTG CGCTACGGCA ACCCCTGGGA GAAGGCCCGG 
CCGGAGTACA TGCTGCCTGT GCACTTCTAC GGGCGGGTGG AGCACTGCCT GGAGGGCGTC 
AAGTGGCTGG ACACCCAGGT CGTGCTGGCC ATGCCCTACG ACACCCCGGT GCCCGGCTAC 
AGGAACAACA CCGTCAACAC TATGAGGCTA TGGTCCGCCA AGGCACCCAA CGACTTCAAG 
CTCCATGACT TTAATGTGGG TGGCTACATC GAGGCAGTCC TGGACAGGAA CCTGGCCGAG 
AACATCTCCA GAGTCCTGTA TCCAAATGAT AATTTCTTCG AGGGGAAGGA GCTCCGCCTG 
AAGCAGGAGT ACTTTGTGGT GGCTGCCACC CTCCAGGACA TCATTCGACG CTTTAAGTCC 
TCCAAGTTCG GCTGCCGGGA CCCAGTGAGA ACCTGTTTTG AGACGTTCCC AGACAAGGTT 
GCCATCCAGC TGAACGACAC CCACCCAGCC CTTGCCATCC CTGAGCTCAT GCGGATACTG 
GTGGACGTGG AGAAAGTGGA CTGGGACAAG GCCTGGGAAA TCACAAAGAA GACCTGTGCA 
TACACCAACC ACACCGTGTT ACCCGAGGCC TTAGAGCGCT GGCCCGTGTC CATGTTTGAG 
AAGCTCCTGC CACGGCACCT GGAGATCATC TACGCCATCA ACCAGAGGCA CCTGGATCAC 
GTGGCCGCCC TGTTCCCTGG GGACGTTGAC CGCCTGCGGA GGATGTCTGT GATCGAGGAA 
GGGGACTGCA AGCGGATTAA CATGGCCCAC CTGTGTGTGA TCGGGTCCCA TGCTGTCAAC 
GGCGTGGCGA GGATCCACTC GGAGATTGTG AAGCAGTCAG TCTTTAAGGA CTTTTATGAG 
CTGGAGCCAG AGAAGTTCCA GAATAAGACA AATGGCATCA CACCCCGACG GTGGCTGCTG 
CTGTGTAACC CGGGGCTGGC CGGCACCATC GCGGAGAAAA TTGGGGAGGG TTTCCTGACT 
GACCTGAGCC AACTGAAGAA GCTGTTGCCA TTGGTTGATG ACGAGGCACT CATCAGGGAT 
GTGGCCAAGG TCAAGCAGGA AAACAAGCTG AAGTTCTCTG CCTTGCTGGA GAAGGAATAT 
AAGGTGAAAA TCAACCCTTC CTCCATGTTC GACGTGCACG TGAAGAGGAT CCATGAGTAC 
AAGCGGCAGC TGCTGAACTG CCTGCATATC GTCACCCTGT ACAACCGAAT AAAGAAGGAC 
CCAGCCAGAG CCTTTGTGCC CAGGACTGTC ATGATCGGGG GCAAGGCAGC CCCCGGTTAC 
CACATGGCGA AGATGATCAT CAAGCTGGTC ACATCCATCG GCAGTGTTGT CAACCACGAT 
CCAGTTGTGG GCGACAAGCT CAAAGTGATC TTCCTGGAAA ACTACCGAGT GTCTTTGGCT 
GAGAAAGTGA TCCCCGCAGC AGACCTTTCC CAGCAGATCT CCACTGCGGG CACCGAGGCC 
TCGGGCACGG GCAACATGAA GTTCATGCTC AACGGGGCCC TCACCATTGG TACCATGGAT 
GGAGCCAACG TGGAGATGGC CGAGGAAGCC GGGGCTGAGA ACCTCTTCAT CTTTGGCATG 
CGTGTGGAGG ACGTCGAGGC CCTGGACCAG AAAGGGTACA ATGCCAGAGA ATACTATGAC 
CACCTGCCCG AGCTGAAGCA GGCCGTGGAC CAGATCAGCA GCGGCTTCTT CTCTCCCAAG 
GAACCAGACT GCTTCAGGGA CATGGTCAAC ATGCTGCTGA ACCACGACAG GTTCAAGGTG 
TTTGCAGATT ATGAAGCGTA CGTGGCATGC CAGGCCCAGG TGGACCAGCT GTACCGGAAC 
CCCAGGGAGT GGACCAAGAA GGTCATCAGG AACATCGCCT GCTCGGGCAA GTTCTCCAGT 
GACCGGACCA TCACAGAGTA CGCCCGGGAC ATCTGGGGCG TGGAGCCCTC CGACCTGCAG 
ATCCCGCCCC CCAACCTCCC CAGGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGB ( XM_006745447.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006745510.1 Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLe113504
Clone ID Related Accession (Same CDS sequence)	XM_006745447.1
Accession Version	XM_006745447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	glycogen phosphorylase, brain form isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006385059.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGAAGATTG TCGATGGCTG GCAGGTGGAG GAGGCCGATG ACTGGCTGCG CTACGGCAAC 
CCCTGGGAGA AGGCCCGGCC GGAGTACATG CTGCCTGTGC ACTTCTACGG GCGGGTGGAG 
CACTGCCTGG AGGGCGTCAA GTGGCTGGAC ACCCAGGTCG TGCTGGCCAT GCCCTACGAC 
ACCCCGGTGC CCGGCTACAG GAACAACACC GTCAACACTA TGAGGCTATG GTCCGCCAAG 
GCACCCAACG ACTTCAAGCT CCATGACTTT AATGTGGGTG GCTACATCGA GGCAGTCCTG 
GACAGGAACC TGGCCGAGAA CATCTCCAGA GTCCTGTATC CAAATGATAA TTTCTTCGAG 
GGGAAGGAGC TCCGCCTGAA GCAGGAGTAC TTTGTGGTGG CTGCCACCCT CCAGGACATC 
ATTCGACGCT TTAAGTCCTC CAAGTTCGGC TGCCGGGACC CAGTGAGAAC CTGTTTTGAG 
ACGTTCCCAG ACAAGGTTGC CATCCAGCTG AACGACACCC ACCCAGCCCT TGCCATCCCT 
GAGCTCATGC GGATACTGGT GGACGTGGAG AAAGTGGACT GGGACAAGGC CTGGGAAATC 
ACAAAGAAGA CCTGTGCATA CACCAACCAC ACCGTGTTAC CCGAGGCCTT AGAGCGCTGG 
CCCGTGTCCA TGTTTGAGAA GCTCCTGCCA CGGCACCTGG AGATCATCTA CGCCATCAAC 
CAGAGGCACC TGGATCACGT GGCCGCCCTG TTCCCTGGGG ACGTTGACCG CCTGCGGAGG 
ATGTCTGTGA TCGAGGAAGG GGACTGCAAG CGGATTAACA TGGCCCACCT GTGTGTGATC 
GGGTCCCATG CTGTCAACGG CGTGGCGAGG ATCCACTCGG AGATTGTGAA GCAGTCAGTC 
TTTAAGGACT TTTATGAGCT GGAGCCAGAG AAGTTCCAGA ATAAGACAAA TGGCATCACA 
CCCCGACGGT GGCTGCTGCT GTGTAACCCG GGGCTGGCCG GCACCATCGC GGAGAAAATT 
GGGGAGGGTT TCCTGACTGA CCTGAGCCAA CTGAAGAAGC TGTTGCCATT GGTTGATGAC 
GAGGCACTCA TCAGGGATGT GGCCAAGGTC AAGCAGGAAA ACAAGCTGAA GTTCTCTGCC 
TTGCTGGAGA AGGAATATAA GGTGAAAATC AACCCTTCCT CCATGTTCGA CGTGCACGTG 
AAGAGGATCC ATGAGTACAA GCGGCAGCTG CTGAACTGCC TGCATATCGT CACCCTGTAC 
AACCGAATAA AGAAGGACCC AGCCAGAGCC TTTGTGCCCA GGACTGTCAT GATCGGGGGC 
AAGGCAGCCC CCGGTTACCA CATGGCGAAG ATGATCATCA AGCTGGTCAC ATCCATCGGC 
AGTGTTGTCA ACCACGATCC AGTTGTGGGC GACAAGCTCA AAGTGATCTT CCTGGAAAAC 
TACCGAGTGT CTTTGGCTGA GAAAGTGATC CCCGCAGCAG ACCTTTCCCA GCAGATCTCC 
ACTGCGGGCA CCGAGGCCTC GGGCACGGGC AACATGAAGT TCATGCTCAA CGGGGCCCTC 
ACCATTGGTA CCATGGATGG AGCCAACGTG GAGATGGCCG AGGAAGCCGG GGCTGAGAAC 
CTCTTCATCT TTGGCATGCG TGTGGAGGAC GTCGAGGCCC TGGACCAGAA AGGGTACAAT 
GCCAGAGAAT ACTATGACCA CCTGCCCGAG CTGAAGCAGG CCGTGGACCA GATCAGCAGC 
GGCTTCTTCT CTCCCAAGGA ACCAGACTGC TTCAGGGACA TGGTCAACAT GCTGCTGAAC 
CACGACAGGT TCAAGGTGTT TGCAGATTAT GAAGCGTACG TGGCATGCCA GGCCCAGGTG 
GACCAGCTGT ACCGGAACCC CAGGGAGTGG ACCAAGAAGG TCATCAGGAA CATCGCCTGC 
TCGGGCAAGT TCTCCAGTGA CCGGACCATC ACAGAGTACG CCCGGGACAT CTGGGGCGTG 
GAGCCCTCCG ACCTGCAGAT CCCGCCCCCC AACCTCCCCA GGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006745510.1
CDS	1..2268
Translation	MAKPLTDSEKRKQISVRGIAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKKIVDGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHCLEGVKWLDTQVVLAMPYDTPVPGYRNNTVNTMRLWSAKAPNDFKLHDFNVGGYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALAIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLAGTIAEKIGEGFLTDLSQLKKLLPLVDDEALIRDVAKVKQENKLKFSALLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHIVTLYNRIKKDPARAFVPRTVMIGGKAAPGYHMAKMIIKLVTSIGSVVNHDPVVGDKLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGMRVEDVEALDQKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFRDMVNMLLNHDRFKVFADYEAYVACQAQVDQLYRNPREWTKKVIRNIACSGKFSSDRTITEYARDIWGVEPSDLQIPPPNLPRD

Target ORF information:

RefSeq Version	XM_006745447.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006745447.1

ORF Insert Sequence:

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGAAGATTG TCGATGGCTG GCAGGTGGAG GAGGCCGATG ACTGGCTGCG CTACGGCAAC 
CCCTGGGAGA AGGCCCGGCC GGAGTACATG CTGCCTGTGC ACTTCTACGG GCGGGTGGAG 
CACTGCCTGG AGGGCGTCAA GTGGCTGGAC ACCCAGGTCG TGCTGGCCAT GCCCTACGAC 
ACCCCGGTGC CCGGCTACAG GAACAACACC GTCAACACTA TGAGGCTATG GTCCGCCAAG 
GCACCCAACG ACTTCAAGCT CCATGACTTT AATGTGGGTG GCTACATCGA GGCAGTCCTG 
GACAGGAACC TGGCCGAGAA CATCTCCAGA GTCCTGTATC CAAATGATAA TTTCTTCGAG 
GGGAAGGAGC TCCGCCTGAA GCAGGAGTAC TTTGTGGTGG CTGCCACCCT CCAGGACATC 
ATTCGACGCT TTAAGTCCTC CAAGTTCGGC TGCCGGGACC CAGTGAGAAC CTGTTTTGAG 
ACGTTCCCAG ACAAGGTTGC CATCCAGCTG AACGACACCC ACCCAGCCCT TGCCATCCCT 
GAGCTCATGC GGATACTGGT GGACGTGGAG AAAGTGGACT GGGACAAGGC CTGGGAAATC 
ACAAAGAAGA CCTGTGCATA CACCAACCAC ACCGTGTTAC CCGAGGCCTT AGAGCGCTGG 
CCCGTGTCCA TGTTTGAGAA GCTCCTGCCA CGGCACCTGG AGATCATCTA CGCCATCAAC 
CAGAGGCACC TGGATCACGT GGCCGCCCTG TTCCCTGGGG ACGTTGACCG CCTGCGGAGG 
ATGTCTGTGA TCGAGGAAGG GGACTGCAAG CGGATTAACA TGGCCCACCT GTGTGTGATC 
GGGTCCCATG CTGTCAACGG CGTGGCGAGG ATCCACTCGG AGATTGTGAA GCAGTCAGTC 
TTTAAGGACT TTTATGAGCT GGAGCCAGAG AAGTTCCAGA ATAAGACAAA TGGCATCACA 
CCCCGACGGT GGCTGCTGCT GTGTAACCCG GGGCTGGCCG GCACCATCGC GGAGAAAATT 
GGGGAGGGTT TCCTGACTGA CCTGAGCCAA CTGAAGAAGC TGTTGCCATT GGTTGATGAC 
GAGGCACTCA TCAGGGATGT GGCCAAGGTC AAGCAGGAAA ACAAGCTGAA GTTCTCTGCC 
TTGCTGGAGA AGGAATATAA GGTGAAAATC AACCCTTCCT CCATGTTCGA CGTGCACGTG 
AAGAGGATCC ATGAGTACAA GCGGCAGCTG CTGAACTGCC TGCATATCGT CACCCTGTAC 
AACCGAATAA AGAAGGACCC AGCCAGAGCC TTTGTGCCCA GGACTGTCAT GATCGGGGGC 
AAGGCAGCCC CCGGTTACCA CATGGCGAAG ATGATCATCA AGCTGGTCAC ATCCATCGGC 
AGTGTTGTCA ACCACGATCC AGTTGTGGGC GACAAGCTCA AAGTGATCTT CCTGGAAAAC 
TACCGAGTGT CTTTGGCTGA GAAAGTGATC CCCGCAGCAG ACCTTTCCCA GCAGATCTCC 
ACTGCGGGCA CCGAGGCCTC GGGCACGGGC AACATGAAGT TCATGCTCAA CGGGGCCCTC 
ACCATTGGTA CCATGGATGG AGCCAACGTG GAGATGGCCG AGGAAGCCGG GGCTGAGAAC 
CTCTTCATCT TTGGCATGCG TGTGGAGGAC GTCGAGGCCC TGGACCAGAA AGGGTACAAT 
GCCAGAGAAT ACTATGACCA CCTGCCCGAG CTGAAGCAGG CCGTGGACCA GATCAGCAGC 
GGCTTCTTCT CTCCCAAGGA ACCAGACTGC TTCAGGGACA TGGTCAACAT GCTGCTGAAC 
CACGACAGGT TCAAGGTGTT TGCAGATTAT GAAGCGTACG TGGCATGCCA GGCCCAGGTG 
GACCAGCTGT ACCGGAACCC CAGGGAGTGG ACCAAGAAGG TCATCAGGAA CATCGCCTGC 
TCGGGCAAGT TCTCCAGTGA CCGGACCATC ACAGAGTACG CCCGGGACAT CTGGGGCGTG 
GAGCCCTCCG ACCTGCAGAT CCCGCCCCCC AACCTCCCCA GGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGB ( XM_006745448.2 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006745511.1 Leptonychotes weddellii glycogen phosphorylase B (PYGB), mRNA.

Price & Availability↑

CloneID	OLe113505
Clone ID Related Accession (Same CDS sequence)	XM_006745448.2 , XM_006745448.1
Accession Version	XM_006745448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Leptonychotes weddellii(Weddell seal)
Product	glycogen phosphorylase, brain form
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006385059.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 30, 2019 this sequence version replaced XM_006745448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Leptonychotes weddellii Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATCT ATTATCTTTC CCTGGAATTC TACATGGGCC GCACACTGCA GAACACAATG 
GTGAACCTGG GTCTTCAGAA TGCTTGTGAT GAAGCCATTT ATCAGTTGGG GTTGGACTTG 
GAAGAACTGG AGGAAATAGA AGAGGACGCT GGCCTCGGGA ACGGAGGCTT GGGGAGGCTG 
GCAGCGTGCT TCCTTGACTC GATGGCCACG TTGGGCCTGG CAGCATATGG CTATGGAATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATTGTCGATG GCTGGCAGGT GGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCGGAGTA CATGCTGCCT 
GTGCACTTCT ACGGGCGGGT GGAGCACTGC CTGGAGGGCG TCAAGTGGCT GGACACCCAG 
GTCGTGCTGG CCATGCCCTA CGACACCCCG GTGCCCGGCT ACAGGAACAA CACCGTCAAC 
ACTATGAGGC TATGGTCCGC CAAGGCACCC AACGACTTCA AGCTCCATGA CTTTAATGTG 
GGTGGCTACA TCGAGGCAGT CCTGGACAGG AACCTGGCCG AGAACATCTC CAGAGTCCTG 
TATCCAAATG ATAATTTCTT CGAGGGGAAG GAGCTCCGCC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATTCGA CGCTTTAAGT CCTCCAAGTT CGGCTGCCGG 
GACCCAGTGA GAACCTGTTT TGAGACGTTC CCAGACAAGG TTGCCATCCA GCTGAACGAC 
ACCCACCCAG CCCTTGCCAT CCCTGAGCTC ATGCGGATAC TGGTGGACGT GGAGAAAGTG 
GACTGGGACA AGGCCTGGGA AATCACAAAG AAGACCTGTG CATACACCAA CCACACCGTG 
TTACCCGAGG CCTTAGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTCCT GCCACGGCAC 
CTGGAGATCA TCTACGCCAT CAACCAGAGG CACCTGGATC ACGTGGCCGC CCTGTTCCCT 
GGGGACGTTG ACCGCCTGCG GAGGATGTCT GTGATCGAGG AAGGGGACTG CAAGCGGATT 
AACATGGCCC ACCTGTGTGT GATCGGGTCC CATGCTGTCA ACGGCGTGGC GAGGATCCAC 
TCGGAGATTG TGAAGCAGTC AGTCTTTAAG GACTTTTATG AGCTGGAGCC AGAGAAGTTC 
CAGAATAAGA CAAATGGCAT CACACCCCGA CGGTGGCTGC TGCTGTGTAA CCCGGGGCTG 
GCCGGCACCA TCGCGGAGAA AATTGGGGAG GGTTTCCTGA CTGACCTGAG CCAACTGAAG 
AAGCTGTTGC CATTGGTTGA TGACGAGGCA CTCATCAGGG ATGTGGCCAA GGTCAAGCAG 
GAAAACAAGC TGAAGTTCTC TGCCTTGCTG GAGAAGGAAT ATAAGGTGAA AATCAACCCT 
TCCTCCATGT TCGACGTGCA CGTGAAGAGG ATCCATGAGT ACAAGCGGCA GCTGCTGAAC 
TGCCTGCATA TCGTCACCCT GTACAACCGA ATAAAGAAGG ACCCAGCCAG AGCCTTTGTG 
CCCAGGACTG TCATGATCGG GGGCAAGGCA GCCCCCGGTT ACCACATGGC GAAGATGATC 
ATCAAGCTGG TCACATCCAT CGGCAGTGTT GTCAACCACG ATCCAGTTGT GGGCGACAAG 
CTCAAAGTGA TCTTCCTGGA AAACTACCGA GTGTCTTTGG CTGAGAAAGT GATCCCCGCA 
GCAGACCTTT CCCAGCAGAT CTCCACTGCG GGCACCGAGG CCTCGGGCAC GGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATT GGTACCATGG ATGGAGCCAA CGTGGAGATG 
GCCGAGGAAG CCGGGGCTGA GAACCTCTTC ATCTTTGGCA TGCGTGTGGA GGACGTCGAG 
GCCCTGGACC AGAAAGGGTA CAATGCCAGA GAATACTATG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGCGGCTTC TTCTCTCCCA AGGAACCAGA CTGCTTCAGG 
GACATGGTCA ACATGCTGCT GAACCACGAC AGGTTCAAGG TGTTTGCAGA TTATGAAGCG 
TACGTGGCAT GCCAGGCCCA GGTGGACCAG CTGTACCGGA ACCCCAGGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACAGAG 
TACGCCCGGG ACATCTGGGG CGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACCTC 
CCCAGGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006745511.1
CDS	120..2651
Translation	MAKPLTDSEKRKQISVRGIAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVDGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHCLEGVKWLDTQVVLAMPYDTPVPGYRNNTVNTMRLWSAKAPNDFKLHDFNVGGYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALAIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLAGTIAEKIGEGFLTDLSQLKKLLPLVDDEALIRDVAKVKQENKLKFSALLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHIVTLYNRIKKDPARAFVPRTVMIGGKAAPGYHMAKMIIKLVTSIGSVVNHDPVVGDKLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGMRVEDVEALDQKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFRDMVNMLLNHDRFKVFADYEAYVACQAQVDQLYRNPREWTKKVIRNIACSGKFSSDRTITEYARDIWGVEPSDLQIPPPNLPRD

Target ORF information:

RefSeq Version	XM_006745448.2
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii glycogen phosphorylase B (PYGB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006745448.2

ORF Insert Sequence:

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATCT ATTATCTTTC CCTGGAATTC TACATGGGCC GCACACTGCA GAACACAATG 
GTGAACCTGG GTCTTCAGAA TGCTTGTGAT GAAGCCATTT ATCAGTTGGG GTTGGACTTG 
GAAGAACTGG AGGAAATAGA AGAGGACGCT GGCCTCGGGA ACGGAGGCTT GGGGAGGCTG 
GCAGCGTGCT TCCTTGACTC GATGGCCACG TTGGGCCTGG CAGCATATGG CTATGGAATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATTGTCGATG GCTGGCAGGT GGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCGGAGTA CATGCTGCCT 
GTGCACTTCT ACGGGCGGGT GGAGCACTGC CTGGAGGGCG TCAAGTGGCT GGACACCCAG 
GTCGTGCTGG CCATGCCCTA CGACACCCCG GTGCCCGGCT ACAGGAACAA CACCGTCAAC 
ACTATGAGGC TATGGTCCGC CAAGGCACCC AACGACTTCA AGCTCCATGA CTTTAATGTG 
GGTGGCTACA TCGAGGCAGT CCTGGACAGG AACCTGGCCG AGAACATCTC CAGAGTCCTG 
TATCCAAATG ATAATTTCTT CGAGGGGAAG GAGCTCCGCC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATTCGA CGCTTTAAGT CCTCCAAGTT CGGCTGCCGG 
GACCCAGTGA GAACCTGTTT TGAGACGTTC CCAGACAAGG TTGCCATCCA GCTGAACGAC 
ACCCACCCAG CCCTTGCCAT CCCTGAGCTC ATGCGGATAC TGGTGGACGT GGAGAAAGTG 
GACTGGGACA AGGCCTGGGA AATCACAAAG AAGACCTGTG CATACACCAA CCACACCGTG 
TTACCCGAGG CCTTAGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTCCT GCCACGGCAC 
CTGGAGATCA TCTACGCCAT CAACCAGAGG CACCTGGATC ACGTGGCCGC CCTGTTCCCT 
GGGGACGTTG ACCGCCTGCG GAGGATGTCT GTGATCGAGG AAGGGGACTG CAAGCGGATT 
AACATGGCCC ACCTGTGTGT GATCGGGTCC CATGCTGTCA ACGGCGTGGC GAGGATCCAC 
TCGGAGATTG TGAAGCAGTC AGTCTTTAAG GACTTTTATG AGCTGGAGCC AGAGAAGTTC 
CAGAATAAGA CAAATGGCAT CACACCCCGA CGGTGGCTGC TGCTGTGTAA CCCGGGGCTG 
GCCGGCACCA TCGCGGAGAA AATTGGGGAG GGTTTCCTGA CTGACCTGAG CCAACTGAAG 
AAGCTGTTGC CATTGGTTGA TGACGAGGCA CTCATCAGGG ATGTGGCCAA GGTCAAGCAG 
GAAAACAAGC TGAAGTTCTC TGCCTTGCTG GAGAAGGAAT ATAAGGTGAA AATCAACCCT 
TCCTCCATGT TCGACGTGCA CGTGAAGAGG ATCCATGAGT ACAAGCGGCA GCTGCTGAAC 
TGCCTGCATA TCGTCACCCT GTACAACCGA ATAAAGAAGG ACCCAGCCAG AGCCTTTGTG 
CCCAGGACTG TCATGATCGG GGGCAAGGCA GCCCCCGGTT ACCACATGGC GAAGATGATC 
ATCAAGCTGG TCACATCCAT CGGCAGTGTT GTCAACCACG ATCCAGTTGT GGGCGACAAG 
CTCAAAGTGA TCTTCCTGGA AAACTACCGA GTGTCTTTGG CTGAGAAAGT GATCCCCGCA 
GCAGACCTTT CCCAGCAGAT CTCCACTGCG GGCACCGAGG CCTCGGGCAC GGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATT GGTACCATGG ATGGAGCCAA CGTGGAGATG 
GCCGAGGAAG CCGGGGCTGA GAACCTCTTC ATCTTTGGCA TGCGTGTGGA GGACGTCGAG 
GCCCTGGACC AGAAAGGGTA CAATGCCAGA GAATACTATG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGCGGCTTC TTCTCTCCCA AGGAACCAGA CTGCTTCAGG 
GACATGGTCA ACATGCTGCT GAACCACGAC AGGTTCAAGG TGTTTGCAGA TTATGAAGCG 
TACGTGGCAT GCCAGGCCCA GGTGGACCAG CTGTACCGGA ACCCCAGGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACAGAG 
TACGCCCGGG ACATCTGGGG CGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACCTC 
CCCAGGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGB ( XM_006745448.1 ) cDNA ORF clone, Leptonychotes weddellii(Weddell seal) -> XP_006745511.1 Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X3, mRNA.

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CloneID	OLe113505
Clone ID Related Accession (Same CDS sequence)	XM_006745448.2 , XM_006745448.1
Accession Version	XM_006745448.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-13
Organism	Leptonychotes weddellii(Weddell seal)
Product	glycogen phosphorylase, brain form isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006385059.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Leptonychotes weddellii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATCT ATTATCTTTC CCTGGAATTC TACATGGGCC GCACACTGCA GAACACAATG 
GTGAACCTGG GTCTTCAGAA TGCTTGTGAT GAAGCCATTT ATCAGTTGGG GTTGGACTTG 
GAAGAACTGG AGGAAATAGA AGAGGACGCT GGCCTCGGGA ACGGAGGCTT GGGGAGGCTG 
GCAGCGTGCT TCCTTGACTC GATGGCCACG TTGGGCCTGG CAGCATATGG CTATGGAATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATTGTCGATG GCTGGCAGGT GGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCGGAGTA CATGCTGCCT 
GTGCACTTCT ACGGGCGGGT GGAGCACTGC CTGGAGGGCG TCAAGTGGCT GGACACCCAG 
GTCGTGCTGG CCATGCCCTA CGACACCCCG GTGCCCGGCT ACAGGAACAA CACCGTCAAC 
ACTATGAGGC TATGGTCCGC CAAGGCACCC AACGACTTCA AGCTCCATGA CTTTAATGTG 
GGTGGCTACA TCGAGGCAGT CCTGGACAGG AACCTGGCCG AGAACATCTC CAGAGTCCTG 
TATCCAAATG ATAATTTCTT CGAGGGGAAG GAGCTCCGCC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATTCGA CGCTTTAAGT CCTCCAAGTT CGGCTGCCGG 
GACCCAGTGA GAACCTGTTT TGAGACGTTC CCAGACAAGG TTGCCATCCA GCTGAACGAC 
ACCCACCCAG CCCTTGCCAT CCCTGAGCTC ATGCGGATAC TGGTGGACGT GGAGAAAGTG 
GACTGGGACA AGGCCTGGGA AATCACAAAG AAGACCTGTG CATACACCAA CCACACCGTG 
TTACCCGAGG CCTTAGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTCCT GCCACGGCAC 
CTGGAGATCA TCTACGCCAT CAACCAGAGG CACCTGGATC ACGTGGCCGC CCTGTTCCCT 
GGGGACGTTG ACCGCCTGCG GAGGATGTCT GTGATCGAGG AAGGGGACTG CAAGCGGATT 
AACATGGCCC ACCTGTGTGT GATCGGGTCC CATGCTGTCA ACGGCGTGGC GAGGATCCAC 
TCGGAGATTG TGAAGCAGTC AGTCTTTAAG GACTTTTATG AGCTGGAGCC AGAGAAGTTC 
CAGAATAAGA CAAATGGCAT CACACCCCGA CGGTGGCTGC TGCTGTGTAA CCCGGGGCTG 
GCCGGCACCA TCGCGGAGAA AATTGGGGAG GGTTTCCTGA CTGACCTGAG CCAACTGAAG 
AAGCTGTTGC CATTGGTTGA TGACGAGGCA CTCATCAGGG ATGTGGCCAA GGTCAAGCAG 
GAAAACAAGC TGAAGTTCTC TGCCTTGCTG GAGAAGGAAT ATAAGGTGAA AATCAACCCT 
TCCTCCATGT TCGACGTGCA CGTGAAGAGG ATCCATGAGT ACAAGCGGCA GCTGCTGAAC 
TGCCTGCATA TCGTCACCCT GTACAACCGA ATAAAGAAGG ACCCAGCCAG AGCCTTTGTG 
CCCAGGACTG TCATGATCGG GGGCAAGGCA GCCCCCGGTT ACCACATGGC GAAGATGATC 
ATCAAGCTGG TCACATCCAT CGGCAGTGTT GTCAACCACG ATCCAGTTGT GGGCGACAAG 
CTCAAAGTGA TCTTCCTGGA AAACTACCGA GTGTCTTTGG CTGAGAAAGT GATCCCCGCA 
GCAGACCTTT CCCAGCAGAT CTCCACTGCG GGCACCGAGG CCTCGGGCAC GGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATT GGTACCATGG ATGGAGCCAA CGTGGAGATG 
GCCGAGGAAG CCGGGGCTGA GAACCTCTTC ATCTTTGGCA TGCGTGTGGA GGACGTCGAG 
GCCCTGGACC AGAAAGGGTA CAATGCCAGA GAATACTATG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGCGGCTTC TTCTCTCCCA AGGAACCAGA CTGCTTCAGG 
GACATGGTCA ACATGCTGCT GAACCACGAC AGGTTCAAGG TGTTTGCAGA TTATGAAGCG 
TACGTGGCAT GCCAGGCCCA GGTGGACCAG CTGTACCGGA ACCCCAGGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACAGAG 
TACGCCCGGG ACATCTGGGG CGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACCTC 
CCCAGGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006745511.1
CDS	13..2544
Translation	MAKPLTDSEKRKQISVRGIAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVDGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHCLEGVKWLDTQVVLAMPYDTPVPGYRNNTVNTMRLWSAKAPNDFKLHDFNVGGYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALAIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLAGTIAEKIGEGFLTDLSQLKKLLPLVDDEALIRDVAKVKQENKLKFSALLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHIVTLYNRIKKDPARAFVPRTVMIGGKAAPGYHMAKMIIKLVTSIGSVVNHDPVVGDKLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGMRVEDVEALDQKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFRDMVNMLLNHDRFKVFADYEAYVACQAQVDQLYRNPREWTKKVIRNIACSGKFSSDRTITEYARDIWGVEPSDLQIPPPNLPRD

Target ORF information:

RefSeq Version	XM_006745448.1
Organism	Leptonychotes weddellii(Weddell seal)
Definition	Leptonychotes weddellii phosphorylase, glycogen; brain (PYGB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006745448.1

ORF Insert Sequence:

ATGGCGAAGC CGCTGACCGA CAGCGAGAAG CGGAAGCAGA TCAGTGTGCG CGGCATCGCG 
GGGCTGGGCG ACGTGGCCGA GGTGCGGAAG AGCTTCAATC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACGCCCCGT GACTACTTCT TCGCGCTGGC TCACACTGTG 
CGGGACCACC TGGTGGGCCG CTGGATCCGC ACGCAGCAGC ACTACTACGA GCGCGACCCC 
AAGCGCATCT ATTATCTTTC CCTGGAATTC TACATGGGCC GCACACTGCA GAACACAATG 
GTGAACCTGG GTCTTCAGAA TGCTTGTGAT GAAGCCATTT ATCAGTTGGG GTTGGACTTG 
GAAGAACTGG AGGAAATAGA AGAGGACGCT GGCCTCGGGA ACGGAGGCTT GGGGAGGCTG 
GCAGCGTGCT TCCTTGACTC GATGGCCACG TTGGGCCTGG CAGCATATGG CTATGGAATC 
CGCTATGAGT TTGGGATTTT TAACCAGAAG ATTGTCGATG GCTGGCAGGT GGAGGAGGCC 
GATGACTGGC TGCGCTACGG CAACCCCTGG GAGAAGGCCC GGCCGGAGTA CATGCTGCCT 
GTGCACTTCT ACGGGCGGGT GGAGCACTGC CTGGAGGGCG TCAAGTGGCT GGACACCCAG 
GTCGTGCTGG CCATGCCCTA CGACACCCCG GTGCCCGGCT ACAGGAACAA CACCGTCAAC 
ACTATGAGGC TATGGTCCGC CAAGGCACCC AACGACTTCA AGCTCCATGA CTTTAATGTG 
GGTGGCTACA TCGAGGCAGT CCTGGACAGG AACCTGGCCG AGAACATCTC CAGAGTCCTG 
TATCCAAATG ATAATTTCTT CGAGGGGAAG GAGCTCCGCC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCCA CCCTCCAGGA CATCATTCGA CGCTTTAAGT CCTCCAAGTT CGGCTGCCGG 
GACCCAGTGA GAACCTGTTT TGAGACGTTC CCAGACAAGG TTGCCATCCA GCTGAACGAC 
ACCCACCCAG CCCTTGCCAT CCCTGAGCTC ATGCGGATAC TGGTGGACGT GGAGAAAGTG 
GACTGGGACA AGGCCTGGGA AATCACAAAG AAGACCTGTG CATACACCAA CCACACCGTG 
TTACCCGAGG CCTTAGAGCG CTGGCCCGTG TCCATGTTTG AGAAGCTCCT GCCACGGCAC 
CTGGAGATCA TCTACGCCAT CAACCAGAGG CACCTGGATC ACGTGGCCGC CCTGTTCCCT 
GGGGACGTTG ACCGCCTGCG GAGGATGTCT GTGATCGAGG AAGGGGACTG CAAGCGGATT 
AACATGGCCC ACCTGTGTGT GATCGGGTCC CATGCTGTCA ACGGCGTGGC GAGGATCCAC 
TCGGAGATTG TGAAGCAGTC AGTCTTTAAG GACTTTTATG AGCTGGAGCC AGAGAAGTTC 
CAGAATAAGA CAAATGGCAT CACACCCCGA CGGTGGCTGC TGCTGTGTAA CCCGGGGCTG 
GCCGGCACCA TCGCGGAGAA AATTGGGGAG GGTTTCCTGA CTGACCTGAG CCAACTGAAG 
AAGCTGTTGC CATTGGTTGA TGACGAGGCA CTCATCAGGG ATGTGGCCAA GGTCAAGCAG 
GAAAACAAGC TGAAGTTCTC TGCCTTGCTG GAGAAGGAAT ATAAGGTGAA AATCAACCCT 
TCCTCCATGT TCGACGTGCA CGTGAAGAGG ATCCATGAGT ACAAGCGGCA GCTGCTGAAC 
TGCCTGCATA TCGTCACCCT GTACAACCGA ATAAAGAAGG ACCCAGCCAG AGCCTTTGTG 
CCCAGGACTG TCATGATCGG GGGCAAGGCA GCCCCCGGTT ACCACATGGC GAAGATGATC 
ATCAAGCTGG TCACATCCAT CGGCAGTGTT GTCAACCACG ATCCAGTTGT GGGCGACAAG 
CTCAAAGTGA TCTTCCTGGA AAACTACCGA GTGTCTTTGG CTGAGAAAGT GATCCCCGCA 
GCAGACCTTT CCCAGCAGAT CTCCACTGCG GGCACCGAGG CCTCGGGCAC GGGCAACATG 
AAGTTCATGC TCAACGGGGC CCTCACCATT GGTACCATGG ATGGAGCCAA CGTGGAGATG 
GCCGAGGAAG CCGGGGCTGA GAACCTCTTC ATCTTTGGCA TGCGTGTGGA GGACGTCGAG 
GCCCTGGACC AGAAAGGGTA CAATGCCAGA GAATACTATG ACCACCTGCC CGAGCTGAAG 
CAGGCCGTGG ACCAGATCAG CAGCGGCTTC TTCTCTCCCA AGGAACCAGA CTGCTTCAGG 
GACATGGTCA ACATGCTGCT GAACCACGAC AGGTTCAAGG TGTTTGCAGA TTATGAAGCG 
TACGTGGCAT GCCAGGCCCA GGTGGACCAG CTGTACCGGA ACCCCAGGGA GTGGACCAAG 
AAGGTCATCA GGAACATCGC CTGCTCGGGC AAGTTCTCCA GTGACCGGAC CATCACAGAG 
TACGCCCGGG ACATCTGGGG CGTGGAGCCC TCCGACCTGC AGATCCCGCC CCCCAACCTC 
CCCAGGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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PYGB cDNA ORF clone, Leptonychotes weddellii(Weddell seal)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム