CDKL5 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDKL5
Entrez Gene ID	108495996
Full Name	cyclin dependent kinase like 5
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017812288.1	XP_017667777.1	cyclin-dependent kinase-like 5 isoform X1
XM_017812289.1	XP_017667778.1	cyclin-dependent kinase-like 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following CDKL5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDKL5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe56492	XM_017812288.1 Latest version!	Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLe56493	XM_017812289.1 Latest version!	Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDKL5 ( XM_017812288.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017667777.1 Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe56492
Clone ID Related Accession (Same CDS sequence)	XM_017812288.1
Accession Version	XM_017812288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	cyclin-dependent kinase-like 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690215.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG AGATCCTTGG GGTTGTAGGT 
GAAGGAGCCT ATGGCGTTGT ACTTAAATGC AGACACAAGG AGACACATGA AATTGTGGCT 
ATCAAGAAAT TCAAAGACAG TGAAGAAAAT GAAGAAGTCA AAGAAACCAC TTTACGAGAG 
CTTAAAATGC TTCGCACTTT GAAACAGGAG AACATTGTGG AGTTGAAGGA AGCCTTCAGA 
AGAAGAGGAA AATTATACTT AGTTTTTGAA TATGTGGAAA AAAATATGCT TGAATTGCTA 
GAAGAAATGC CAAATGGAGT TCCACCAGAA AAAGTGAAAA GCTACATCTA CCAGCTAATT 
AAAGCAATTC ATTGGTGCCA TAAGAATGAT ATTGTTCATA GAGATATCAA ACCAGAGAAT 
CTCTTAATAA GCCACAATGA TGTTCTGAAG TTGTGTGACT TTGGCTTTGC TCGTAACCTC 
TCTGAAGGAA GCAATGCTAA CTATACAGAA TATGTGGCTA CCAGATGGTA CCGCTCACCT 
GAACTCTTGC TTGGAGCCCC TTATGGAAAG GCTGTGGATA TGTGGTCTGT AGGCTGTATC 
CTGGGAGAGC TGAGTGATGG GCAGCCCTTG TTTCCTGGTG AGAGTGAGAT TGACCAGCTC 
TTTACCATTC AGAAGGTGCT GGGCCCGCTG CCAGCTGAGC AGATGAAGCT CTTCTATAGC 
AACCCACGCT TCCATGGCTT GAGGTTTCCA GCAGTCAATC ATCCACAGTC TTTAGAGAGA 
AGATACTTGG GAATTTTAAG TGGTGTATTG CTTGACCTTA TGAAGAACCT ACTGAAGTTA 
GATCCAGCAG ATAGATATTT GACAGAACAA TGTTTGAACC ATCCTTCGTT TCAAACCCAA 
AGACTCTTGG ATCGCTGTGG AAGTTCCCCT TCACGGTCAG CTAAGAGAAA ACCTTACCAT 
GCAGACAGCA ACACATTATC TAACAGAAAT CAAGCCAGCA AAAGTTCTGC TTTGCAGTCA 
CACCACAGAT CAAACAGCAA GGATATGCAG ACACTAGGGG CTGGCGGGCC AAGAGAAGAG 
GGTCTTCCAG CAAATGAAAG CTTTTTAAAT GGAAACCTTC CTGGACCTGC AATTAGTCCA 
ATGCATGCAA AAAAACCAAG CAACACACCT GGATCTGGCA ACAAGGATAT AGCCAATAAT 
AACATGCCAC CACACCTTCT CAGCCCAAAG GACACAAAGG GAAAAACAGA ATTTGATTTT 
AATGTGGACA CCAAAAGTTC TGACAGTTCA GGTACAAAGT ACCTGAAGTC CAACACCAGA 
TCTCAACAGA ACCGGCACTC ATTTATGGAA ACCTCTCAAA GCAAAACTGG GACGCTGCAG 
CCCGGCGACA AGCACAGCCG CCACAGCTAC ATCGACACTA TCCCACAGTC TTCTAAGAGT 
CCTTCCTATC GAACAAAGTC AAAGAGCCAT GGAGTTCTGA CAGACTCTAA ATCCGTGGGC 
AACCTTTCTG AGGCCAGGGC CCAAGCTGCA GAACCAAACA CCAGTAGGTA TTTTCCATCC 
AGCTGTATGG ACTTGAACTC TCCTACCAGT CCAAGTGCTC CCCGACACGG TGATAACAGA 
ACTTTGCTCA GTCCGTCTGG GAGGAATAAC CGCAGTGAGG GCACCCTGGA CACCCGAAGA 
CCATCAACTA GACACTCCAA AACAATGGAG GAGTTAAAAC TGCCAGATCA CATGGATGGA 
AGCCATTCCC ACTCTCTATC TGCTCCACAT GAATCTTTTT CCTATGGTCT AGGTTATACC 
AGTCCTTTTT CTTCACAGCA GCGCCCTCAT AGACACTCTA TGTATGTGAG CCGGGACAGA 
GTGAGGTCAA AGGGCTCAGA GGGTGGCTTA AGCATAGGGC AAGGGATGGC AGCCAGAGCA 
AACAGCCTGC AACTGTTATC TCCACAGGTG CAGCATAAGT CACTCACAAG ATCTGTTGGC 
TCATCACGAG AAGACTGTAC GGAAGATTCT AATAGGGGTG GAGCATATCA TGATCCACAC 
ACAGAAGATG GAGCATCTAC TAAGGAAAAT AGAATTCTGT ATAATGACCC TGTTCCGAGA 
AGAGTTGGCA GCTTTTACAG AGTTCCATCT CCGCGTCCAG AGAGTACGTA CATAGAAAAC 
AACGTGTCAA ACAGAGCCTC TGTGTTACCA GCAGACAGCA GCACGGTGGC AAACCACTCA 
AAAAGACAAA CCACCTTTGA TACCTGGAAA AGTCCTGAAA ACCTTAACTC AGAACAGCTC 
AAGGAGAAAG AAAAGCAAGG TTTTTTCAGG GCAATAAAAA AGAAAAAGAA GAAATCTCAA 
ACTACAGACT CAACCAACGG GGAGAATCCA AGCATCAAGA AATCCCTCTT TCCTCTTTTT 
AATTCAAAGA ATAATTTAAA GCATAGTTCT TCTTTGAAAA AGCTTCCTGT AGTCACTCTA 
CCCATGATGC CTAACACTGA TGGTCAGGAT CTCTTGGCAT TACAGAAAGC CATTCATTCT 
TCTAATCACC AGAGCAGCAG GCAGAAGGAT TGGCATCCTG AGAAGATGAC AGAAATCCAG 
CCTCATAGCC AGCCATTAAA ATCTCTTCGA AAGCTCTTAC ACCTCTCTTC AAACCATCCT 
GTCCCTGCTG AGCCCCGCTT CCAGCCCTTA CCAAGTCAGC CAACCAAAGC TTCTTTTTCT 
GAAGTGCGGA TTCACCCCAT GAGTCAGAGC TCCAGCAGCG GCGGCAGCAG CAACGTGCGG 
CAGGAGGCTC CGCCCAAAAG CAGGCCAACA CTGCAGATCC CGAGCCAGCT GGAACCCACC 
TGGCACGTCT CCCCCGTGAA CAGGAGCGTG AGTGATGGGC CTCCCTACTC CGACCAGCTG 
CCTTCCAAGA GCGGACAGAA CGGGCACACG TACACCCGGA CAAACCGGTC GCGAATGCCA 
AACCTGAATG ACTTAAAAGA GACAGCCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017667777.1
CDS	230..3202
Translation	MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGSNANYTEYVATRWYRSPELLLGAPYGKAVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPAEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILSGVLLDLMKNLLKLDPADRYLTEQCLNHPSFQTQRLLDRCGSSPSRSAKRKPYHADSNTLSNRNQASKSSALQSHHRSNSKDMQTLGAGGPREEGLPANESFLNGNLPGPAISPMHAKKPSNTPGSGNKDIANNNMPPHLLSPKDTKGKTEFDFNVDTKSSDSSGTKYLKSNTRSQQNRHSFMETSQSKTGTLQPGDKHSRHSYIDTIPQSSKSPSYRTKSKSHGVLTDSKSVGNLSEARAQAAEPNTSRYFPSSCMDLNSPTSPSAPRHGDNRTLLSPSGRNNRSEGTLDTRRPSTRHSKTMEELKLPDHMDGSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVSRDRVRSKGSEGGLSIGQGMAARANSLQLLSPQVQHKSLTRSVGSSREDCTEDSNRGGAYHDPHTEDGASTKENRILYNDPVPRRVGSFYRVPSPRPESTYIENNVSNRASVLPADSSTVANHSKRQTTFDTWKSPENLNSEQLKEKEKQGFFRAIKKKKKKSQTTDSTNGENPSIKKSLFPLFNSKNNLKHSSSLKKLPVVTLPMMPNTDGQDLLALQKAIHSSNHQSSRQKDWHPEKMTEIQPHSQPLKSLRKLLHLSSNHPVPAEPRFQPLPSQPTKASFSEVRIHPMSQSSSSGGSSNVRQEAPPKSRPTLQIPSQLEPTWHVSPVNRSVSDGPPYSDQLPSKSGQNGHTYTRTNRSRMPNLNDLKETAL

Target ORF information:

RefSeq Version	XM_017812288.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017812288.1

ORF Insert Sequence:

ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG AGATCCTTGG GGTTGTAGGT 
GAAGGAGCCT ATGGCGTTGT ACTTAAATGC AGACACAAGG AGACACATGA AATTGTGGCT 
ATCAAGAAAT TCAAAGACAG TGAAGAAAAT GAAGAAGTCA AAGAAACCAC TTTACGAGAG 
CTTAAAATGC TTCGCACTTT GAAACAGGAG AACATTGTGG AGTTGAAGGA AGCCTTCAGA 
AGAAGAGGAA AATTATACTT AGTTTTTGAA TATGTGGAAA AAAATATGCT TGAATTGCTA 
GAAGAAATGC CAAATGGAGT TCCACCAGAA AAAGTGAAAA GCTACATCTA CCAGCTAATT 
AAAGCAATTC ATTGGTGCCA TAAGAATGAT ATTGTTCATA GAGATATCAA ACCAGAGAAT 
CTCTTAATAA GCCACAATGA TGTTCTGAAG TTGTGTGACT TTGGCTTTGC TCGTAACCTC 
TCTGAAGGAA GCAATGCTAA CTATACAGAA TATGTGGCTA CCAGATGGTA CCGCTCACCT 
GAACTCTTGC TTGGAGCCCC TTATGGAAAG GCTGTGGATA TGTGGTCTGT AGGCTGTATC 
CTGGGAGAGC TGAGTGATGG GCAGCCCTTG TTTCCTGGTG AGAGTGAGAT TGACCAGCTC 
TTTACCATTC AGAAGGTGCT GGGCCCGCTG CCAGCTGAGC AGATGAAGCT CTTCTATAGC 
AACCCACGCT TCCATGGCTT GAGGTTTCCA GCAGTCAATC ATCCACAGTC TTTAGAGAGA 
AGATACTTGG GAATTTTAAG TGGTGTATTG CTTGACCTTA TGAAGAACCT ACTGAAGTTA 
GATCCAGCAG ATAGATATTT GACAGAACAA TGTTTGAACC ATCCTTCGTT TCAAACCCAA 
AGACTCTTGG ATCGCTGTGG AAGTTCCCCT TCACGGTCAG CTAAGAGAAA ACCTTACCAT 
GCAGACAGCA ACACATTATC TAACAGAAAT CAAGCCAGCA AAAGTTCTGC TTTGCAGTCA 
CACCACAGAT CAAACAGCAA GGATATGCAG ACACTAGGGG CTGGCGGGCC AAGAGAAGAG 
GGTCTTCCAG CAAATGAAAG CTTTTTAAAT GGAAACCTTC CTGGACCTGC AATTAGTCCA 
ATGCATGCAA AAAAACCAAG CAACACACCT GGATCTGGCA ACAAGGATAT AGCCAATAAT 
AACATGCCAC CACACCTTCT CAGCCCAAAG GACACAAAGG GAAAAACAGA ATTTGATTTT 
AATGTGGACA CCAAAAGTTC TGACAGTTCA GGTACAAAGT ACCTGAAGTC CAACACCAGA 
TCTCAACAGA ACCGGCACTC ATTTATGGAA ACCTCTCAAA GCAAAACTGG GACGCTGCAG 
CCCGGCGACA AGCACAGCCG CCACAGCTAC ATCGACACTA TCCCACAGTC TTCTAAGAGT 
CCTTCCTATC GAACAAAGTC AAAGAGCCAT GGAGTTCTGA CAGACTCTAA ATCCGTGGGC 
AACCTTTCTG AGGCCAGGGC CCAAGCTGCA GAACCAAACA CCAGTAGGTA TTTTCCATCC 
AGCTGTATGG ACTTGAACTC TCCTACCAGT CCAAGTGCTC CCCGACACGG TGATAACAGA 
ACTTTGCTCA GTCCGTCTGG GAGGAATAAC CGCAGTGAGG GCACCCTGGA CACCCGAAGA 
CCATCAACTA GACACTCCAA AACAATGGAG GAGTTAAAAC TGCCAGATCA CATGGATGGA 
AGCCATTCCC ACTCTCTATC TGCTCCACAT GAATCTTTTT CCTATGGTCT AGGTTATACC 
AGTCCTTTTT CTTCACAGCA GCGCCCTCAT AGACACTCTA TGTATGTGAG CCGGGACAGA 
GTGAGGTCAA AGGGCTCAGA GGGTGGCTTA AGCATAGGGC AAGGGATGGC AGCCAGAGCA 
AACAGCCTGC AACTGTTATC TCCACAGGTG CAGCATAAGT CACTCACAAG ATCTGTTGGC 
TCATCACGAG AAGACTGTAC GGAAGATTCT AATAGGGGTG GAGCATATCA TGATCCACAC 
ACAGAAGATG GAGCATCTAC TAAGGAAAAT AGAATTCTGT ATAATGACCC TGTTCCGAGA 
AGAGTTGGCA GCTTTTACAG AGTTCCATCT CCGCGTCCAG AGAGTACGTA CATAGAAAAC 
AACGTGTCAA ACAGAGCCTC TGTGTTACCA GCAGACAGCA GCACGGTGGC AAACCACTCA 
AAAAGACAAA CCACCTTTGA TACCTGGAAA AGTCCTGAAA ACCTTAACTC AGAACAGCTC 
AAGGAGAAAG AAAAGCAAGG TTTTTTCAGG GCAATAAAAA AGAAAAAGAA GAAATCTCAA 
ACTACAGACT CAACCAACGG GGAGAATCCA AGCATCAAGA AATCCCTCTT TCCTCTTTTT 
AATTCAAAGA ATAATTTAAA GCATAGTTCT TCTTTGAAAA AGCTTCCTGT AGTCACTCTA 
CCCATGATGC CTAACACTGA TGGTCAGGAT CTCTTGGCAT TACAGAAAGC CATTCATTCT 
TCTAATCACC AGAGCAGCAG GCAGAAGGAT TGGCATCCTG AGAAGATGAC AGAAATCCAG 
CCTCATAGCC AGCCATTAAA ATCTCTTCGA AAGCTCTTAC ACCTCTCTTC AAACCATCCT 
GTCCCTGCTG AGCCCCGCTT CCAGCCCTTA CCAAGTCAGC CAACCAAAGC TTCTTTTTCT 
GAAGTGCGGA TTCACCCCAT GAGTCAGAGC TCCAGCAGCG GCGGCAGCAG CAACGTGCGG 
CAGGAGGCTC CGCCCAAAAG CAGGCCAACA CTGCAGATCC CGAGCCAGCT GGAACCCACC 
TGGCACGTCT CCCCCGTGAA CAGGAGCGTG AGTGATGGGC CTCCCTACTC CGACCAGCTG 
CCTTCCAAGA GCGGACAGAA CGGGCACACG TACACCCGGA CAAACCGGTC GCGAATGCCA 
AACCTGAATG ACTTAAAAGA GACAGCCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDKL5 ( XM_017812289.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017667778.1 Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLe56493
Clone ID Related Accession (Same CDS sequence)	XM_017812289.1
Accession Version	XM_017812289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	cyclin-dependent kinase-like 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690215.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG AGATCCTTGG GGTTGTAGGT 
GAAGGAGCCT ATGGCGTTGT ACTTAAATGC AGACACAAGG AGACACATGA AATTGTGGCT 
ATCAAGAAAT TCAAAGACAG TGAAGAAAAT GAAGAAGTCA AAGAAACCAC TTTACGAGAG 
CTTAAAATGC TTCGCACTTT GAAACAGGAG AACATTGTGG AGTTGAAGGA AGCCTTCAGA 
AGAAGAGGAA AATTATACTT AGTTTTTGAA TATGTGGAAA AAAATATGCT TGAATTGCTA 
GAAGAAATGC CAAATGGAGT TCCACCAGAA AAAGTGAAAA GCTACATCTA CCAGCTAATT 
AAAGCAATTC ATTGGTGCCA TAAGAATGAT ATTGTTCATA GAGATATCAA ACCAGAGAAT 
CTCTTAATAA GCCACAATGA TGTTCTGAAG TTGTGTGACT TTGGCTTTGC TCGTAACCTC 
TCTGAAGGAA GCAATGCTAA CTATACAGAA TATGTGGCTA CCAGATGGTA CCGCTCACCT 
GAACTCTTGC TTGGAGCCCC TTATGGAAAG GCTGTGGATA TGTGGTCTGT AGGCTGTATC 
CTGGGAGAGC TGAGTGATGG GCAGCCCTTG TTTCCTGGTG AGAGTGAGAT TGACCAGCTC 
TTTACCATTC AGAAGGTGCT GGGCCCGCTG CCAGCTGAGC AGATGAAGCT CTTCTATAGC 
AACCCACGCT TCCATGGCTT GAGGTTTCCA GCAGTCAATC ATCCACAGTC TTTAGAGAGA 
AGATACTTGG GAATTTTAAG TGGTGTATTG CTTGACCTTA TGAAGAACCT ACTGAAGTTA 
GATCCAGCAG ATAGATATTT GACAGAACAA TGTTTGAACC ATCCTTCGTT TCAAACCCAA 
AGACTCTTGG ATCGCTGTGG AAGTTCCCCT TCACGGTCAG CTAAGAGAAA ACCTTACCAT 
GCAGACAGCA ACACATTATC TAACAGAAAT CAAGCCAGCA AAAGTTCTGC TTTGCAGTCA 
CACCACAGAT CAAACAGCAA GGATATGCAG ACACTAGGGG CTGGCGGGCC AAGAGAAGAG 
GGTCTTCCAG CAAATGAAAG CTTTTTAAAT GGAAACCTTC CTGGACCTGC AATTAGTCCA 
ATGCATGCAA AAAAACCAAG CAACACACCT GGATCTGGCA ACAAGGATAT AGCCAATAAT 
AACATGCCAC CACACCTTCT CAGCCCAAAG GACACAAAGG GAAAAACAGA ATTTGATTTT 
AATGTGGACA CCAAAAGTTC TGACAGTTCA GGTACAAAGT ACCTGAAGTC CAACACCAGA 
TCTCAACAGA ACCGGCACTC ATTTATGGAA ACCTCTCAAA GCAAAACTGG GACGCTGCAG 
CCCGGCGACA AGCACAGCCG CCACAGCTAC ATCGACACTA TCCCACAGTC TTCTAAGAGT 
CCTTCCTATC GAACAAAGTC AAAGAGCCAT GGAGTTCTGA CAGACTCTAA ATCCGTGGGC 
AACCTTTCTG AGGCCAGGGC CCAAGCTGCA GAACCAAACA CCAGTAGGTA TTTTCCATCC 
AGCTGTATGG ACTTGAACTC TCCTACCAGT CCAAGTGCTC CCCGACACGG TGATAACAGA 
ACTTTGCTCA GTCCGTCTGG GAGGAATAAC CGCAGTGAGG GCACCCTGGA CACCCGAAGA 
CCATCAACTA GACACTCCAA AACAATGGAG GAGTTAAAAC TGCCAGATCA CATGGATGGA 
AGCCATTCCC ACTCTCTATC TGCTCCACAT GAATCTTTTT CCTATGGTCT AGGTTATACC 
AGTCCTTTTT CTTCACAGCA GCGCCCTCAT AGACACTCTA TGTATGTGAG CCGGGACAGA 
GTGAGGTCAA AGGGCTCAGA GGGTGGCTTA AGCATAGGGC AAGGGATGGC AGCCAGAGCA 
AACAGCCTGC AACTGTTATC TCCACAGGTG CAGCATAAGT CACTCACAAG ATCTGTTGGC 
TCATCACGAG AAGACTGTAC GGAAGATTCT AATAGGGGTG GAGCATATCA TGATCCACAC 
ACAGAAGATG GAGCATCTAC TAAGGAAAAT AGAATTCTGT ATAATGACCC TGTTCCGAGA 
AGAGTTGGCA GCTTTTACAG AGTTCCATCT CCGCGTCCAG AGAGTACGTA CATAGAAAAC 
AACGTGTCAA ACAGAGCCTC TGTGTTACCA GCAGACAGCA GCACGGTGGC AAACCACTCA 
AAAAGACAAA CCACCTTTGA TACCTGGAAA AGTCCTGAAA ACCTTAACTC AGAACAGCTC 
AAGGAGAAAG AAAAGCAAGG TTTTTTCAGG GCAATAAAAA AGAAAAAGAA GAAATCTCAA 
ACTATGCCTA ACACTGATGG TCAGGATCTC TTGGCATTAC AGAAAGCCAT TCATTCTTCT 
AATCACCAGA GCAGCAGGCA GAAGGATTGG CATCCTGAGA AGATGACAGA AATCCAGCCT 
CATAGCCAGC CATTAAAATC TCTTCGAAAG CTCTTACACC TCTCTTCAAA CCATCCTGTC 
CCTGCTGAGC CCCGCTTCCA GCCCTTACCA AGTCAGCCAA CCAAAGCTTC TTTTTCTGAA 
GTGCGGATTC ACCCCATGAG TCAGAGCTCC AGCAGCGGCG GCAGCAGCAA CGTGCGGCAG 
GAGGCTCCGC CCAAAAGCAG GCCAACACTG CAGATCCCGA GCCAGCTGGA ACCCACCTGG 
CACGTCTCCC CCGTGAACAG GAGCGTGAGT GATGGGCCTC CCTACTCCGA CCAGCTGCCT 
TCCAAGAGCG GACAGAACGG GCACACGTAC ACCCGGACAA ACCGGTCGCG AATGCCAAAC 
CTGAATGACT TAAAAGAGAC AGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017667778.1
CDS	230..3079
Translation	MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGSNANYTEYVATRWYRSPELLLGAPYGKAVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPAEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILSGVLLDLMKNLLKLDPADRYLTEQCLNHPSFQTQRLLDRCGSSPSRSAKRKPYHADSNTLSNRNQASKSSALQSHHRSNSKDMQTLGAGGPREEGLPANESFLNGNLPGPAISPMHAKKPSNTPGSGNKDIANNNMPPHLLSPKDTKGKTEFDFNVDTKSSDSSGTKYLKSNTRSQQNRHSFMETSQSKTGTLQPGDKHSRHSYIDTIPQSSKSPSYRTKSKSHGVLTDSKSVGNLSEARAQAAEPNTSRYFPSSCMDLNSPTSPSAPRHGDNRTLLSPSGRNNRSEGTLDTRRPSTRHSKTMEELKLPDHMDGSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVSRDRVRSKGSEGGLSIGQGMAARANSLQLLSPQVQHKSLTRSVGSSREDCTEDSNRGGAYHDPHTEDGASTKENRILYNDPVPRRVGSFYRVPSPRPESTYIENNVSNRASVLPADSSTVANHSKRQTTFDTWKSPENLNSEQLKEKEKQGFFRAIKKKKKKSQTMPNTDGQDLLALQKAIHSSNHQSSRQKDWHPEKMTEIQPHSQPLKSLRKLLHLSSNHPVPAEPRFQPLPSQPTKASFSEVRIHPMSQSSSSGGSSNVRQEAPPKSRPTLQIPSQLEPTWHVSPVNRSVSDGPPYSDQLPSKSGQNGHTYTRTNRSRMPNLNDLKETAL

Target ORF information:

RefSeq Version	XM_017812289.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata cyclin dependent kinase like 5 (CDKL5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017812289.1

ORF Insert Sequence:

ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG AGATCCTTGG GGTTGTAGGT 
GAAGGAGCCT ATGGCGTTGT ACTTAAATGC AGACACAAGG AGACACATGA AATTGTGGCT 
ATCAAGAAAT TCAAAGACAG TGAAGAAAAT GAAGAAGTCA AAGAAACCAC TTTACGAGAG 
CTTAAAATGC TTCGCACTTT GAAACAGGAG AACATTGTGG AGTTGAAGGA AGCCTTCAGA 
AGAAGAGGAA AATTATACTT AGTTTTTGAA TATGTGGAAA AAAATATGCT TGAATTGCTA 
GAAGAAATGC CAAATGGAGT TCCACCAGAA AAAGTGAAAA GCTACATCTA CCAGCTAATT 
AAAGCAATTC ATTGGTGCCA TAAGAATGAT ATTGTTCATA GAGATATCAA ACCAGAGAAT 
CTCTTAATAA GCCACAATGA TGTTCTGAAG TTGTGTGACT TTGGCTTTGC TCGTAACCTC 
TCTGAAGGAA GCAATGCTAA CTATACAGAA TATGTGGCTA CCAGATGGTA CCGCTCACCT 
GAACTCTTGC TTGGAGCCCC TTATGGAAAG GCTGTGGATA TGTGGTCTGT AGGCTGTATC 
CTGGGAGAGC TGAGTGATGG GCAGCCCTTG TTTCCTGGTG AGAGTGAGAT TGACCAGCTC 
TTTACCATTC AGAAGGTGCT GGGCCCGCTG CCAGCTGAGC AGATGAAGCT CTTCTATAGC 
AACCCACGCT TCCATGGCTT GAGGTTTCCA GCAGTCAATC ATCCACAGTC TTTAGAGAGA 
AGATACTTGG GAATTTTAAG TGGTGTATTG CTTGACCTTA TGAAGAACCT ACTGAAGTTA 
GATCCAGCAG ATAGATATTT GACAGAACAA TGTTTGAACC ATCCTTCGTT TCAAACCCAA 
AGACTCTTGG ATCGCTGTGG AAGTTCCCCT TCACGGTCAG CTAAGAGAAA ACCTTACCAT 
GCAGACAGCA ACACATTATC TAACAGAAAT CAAGCCAGCA AAAGTTCTGC TTTGCAGTCA 
CACCACAGAT CAAACAGCAA GGATATGCAG ACACTAGGGG CTGGCGGGCC AAGAGAAGAG 
GGTCTTCCAG CAAATGAAAG CTTTTTAAAT GGAAACCTTC CTGGACCTGC AATTAGTCCA 
ATGCATGCAA AAAAACCAAG CAACACACCT GGATCTGGCA ACAAGGATAT AGCCAATAAT 
AACATGCCAC CACACCTTCT CAGCCCAAAG GACACAAAGG GAAAAACAGA ATTTGATTTT 
AATGTGGACA CCAAAAGTTC TGACAGTTCA GGTACAAAGT ACCTGAAGTC CAACACCAGA 
TCTCAACAGA ACCGGCACTC ATTTATGGAA ACCTCTCAAA GCAAAACTGG GACGCTGCAG 
CCCGGCGACA AGCACAGCCG CCACAGCTAC ATCGACACTA TCCCACAGTC TTCTAAGAGT 
CCTTCCTATC GAACAAAGTC AAAGAGCCAT GGAGTTCTGA CAGACTCTAA ATCCGTGGGC 
AACCTTTCTG AGGCCAGGGC CCAAGCTGCA GAACCAAACA CCAGTAGGTA TTTTCCATCC 
AGCTGTATGG ACTTGAACTC TCCTACCAGT CCAAGTGCTC CCCGACACGG TGATAACAGA 
ACTTTGCTCA GTCCGTCTGG GAGGAATAAC CGCAGTGAGG GCACCCTGGA CACCCGAAGA 
CCATCAACTA GACACTCCAA AACAATGGAG GAGTTAAAAC TGCCAGATCA CATGGATGGA 
AGCCATTCCC ACTCTCTATC TGCTCCACAT GAATCTTTTT CCTATGGTCT AGGTTATACC 
AGTCCTTTTT CTTCACAGCA GCGCCCTCAT AGACACTCTA TGTATGTGAG CCGGGACAGA 
GTGAGGTCAA AGGGCTCAGA GGGTGGCTTA AGCATAGGGC AAGGGATGGC AGCCAGAGCA 
AACAGCCTGC AACTGTTATC TCCACAGGTG CAGCATAAGT CACTCACAAG ATCTGTTGGC 
TCATCACGAG AAGACTGTAC GGAAGATTCT AATAGGGGTG GAGCATATCA TGATCCACAC 
ACAGAAGATG GAGCATCTAC TAAGGAAAAT AGAATTCTGT ATAATGACCC TGTTCCGAGA 
AGAGTTGGCA GCTTTTACAG AGTTCCATCT CCGCGTCCAG AGAGTACGTA CATAGAAAAC 
AACGTGTCAA ACAGAGCCTC TGTGTTACCA GCAGACAGCA GCACGGTGGC AAACCACTCA 
AAAAGACAAA CCACCTTTGA TACCTGGAAA AGTCCTGAAA ACCTTAACTC AGAACAGCTC 
AAGGAGAAAG AAAAGCAAGG TTTTTTCAGG GCAATAAAAA AGAAAAAGAA GAAATCTCAA 
ACTATGCCTA ACACTGATGG TCAGGATCTC TTGGCATTAC AGAAAGCCAT TCATTCTTCT 
AATCACCAGA GCAGCAGGCA GAAGGATTGG CATCCTGAGA AGATGACAGA AATCCAGCCT 
CATAGCCAGC CATTAAAATC TCTTCGAAAG CTCTTACACC TCTCTTCAAA CCATCCTGTC 
CCTGCTGAGC CCCGCTTCCA GCCCTTACCA AGTCAGCCAA CCAAAGCTTC TTTTTCTGAA 
GTGCGGATTC ACCCCATGAG TCAGAGCTCC AGCAGCGGCG GCAGCAGCAA CGTGCGGCAG 
GAGGCTCCGC CCAAAAGCAG GCCAACACTG CAGATCCCGA GCCAGCTGGA ACCCACCTGG 
CACGTCTCCC CCGTGAACAG GAGCGTGAGT GATGGGCCTC CCTACTCCGA CCAGCTGCCT 
TCCAAGAGCG GACAGAACGG GCACACGTAC ACCCGGACAA ACCGGTCGCG AATGCCAAAC 
CTGAATGACT TAAAAGAGAC AGCCTTGTAA