ALPK1 cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPK1
Entrez Gene ID	102358733
Full Name	alpha kinase 1
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006011359.2	XP_006011421.1	alpha-protein kinase 1

Pathways from BioSystems
Gene Ontology

The following ALPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa42458	XM_006011359.2 Latest version!	Latimeria chalumnae alpha-kinase 1 (ALPK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPK1 ( XM_006011359.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006011421.1 Latimeria chalumnae alpha-kinase 1 (ALPK1), mRNA.

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CloneID	OLa42458
Clone ID Related Accession (Same CDS sequence)	XM_006011359.2
Accession Version	XM_006011359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	alpha-protein kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005821717.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006011359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGAAGTTGT GACCATACTA CAGGAGTGCA AGCAAGCTTT GGACGCGCTC 
TCATCAGGGA CGTCAGAACC AGCCGAGGAG GACAAGAAAG TGTACCAACG GTGTGAAGCT 
TCACTCTCTG ATGATCTGAG GACCCTGCTT CAGGAGGCAA AGGAAATGAA GTGGCCCTTT 
GTGCCAGAGC GATGGCAATA CAAACAAGCC TTATGCCCAG AAGACAAAAC AAATCTGAAA 
GATATGATCA GTGCAAAGCT GCATGAGCTG CTGGTTTATT TGAAAGCCTC CATTGTGGTG 
AAGGATTGCA CTACAGCAGC TGCCGTTGTG TTTTTGATCG ACCGTTTTCT GTACTGGGTA 
GATGCTTCCA GCAGGCTGCT GCAGGTTGCT AAGGGCCTGC ACAAGCTTTG CCCTACAACA 
CCGATCGCGC CACAGGTGGT CATTCGACAG GCCCGCGTCT CTGTAAATTC AGGCAAGCTC 
TTGAAAGCTG AGTACATCCT CAGCAGCCTC ATCAACAACA ACGGTGCGAC AGGATCATGG 
ACATATGCCA AGGAAAGTGA CAGAGTCCTT GTGCAGTCTG TCAGCATTCA GATCAGAGGA 
CAGATTCTAC TAAAACTTGG AATGTGGTAT GAGGCTGCAG AGTTGATTTG GGCCTCGATA 
GTTGGATATT TCGAACTTCC TTTACCAGAT AAAAAGGGCA TAGCAACTTC TTTGGGCATT 
TTGGCTGACA TATTTGTGTC CATGAATGAT AAAGATTACG ACGCGTTTAA AAATAATCCT 
CGGGTTGACT TGTGTCTGCT TAAAGAGTTT GGGCATCGCC TGTTGTCAGC AGCAGAAGCA 
GCCAAGCTGG CTGCTGTGTT CAGCCAGTAC ACGTCGCTCT TTGTGCTCAC TAGTACGAAC 
ATTCGTGGGA CATGTTTATT ATCCTATAGT TTCTCAAGTG ATTGTCCACC AGAGAAGAAA 
AAACATTATC TCTCAGAAGC CAAGGAGGCC TTTGAAGTTA GCCTCCTCAC AAAGAAAGAT 
AATGAAGTAG TTACAAGCAA GCAGGAGCTT CACAGTTTTG TTAAGGCTGC ATTCTGCTTA 
ACGGCAGCAC ACAAGGGCCT CAGCAGTGAA AAAGAAACAC TCCAGGAAGC CAGACAGATG 
TGTAAAGCAG CAATGGAAAA ACTGTACTCC TACGGCACCT TGTCAGAAAC AGTGGAAAAG 
AGGACACTTG CCGAGGAGAT CATGGCGTTG GTGAAGAAAG TTAAAGATCG TTTGCAGGTC 
AGGCCTTTTC CAAATTCAGA TGACAGATCT TATATTCCCG ATAGCTATAA AGTTTGTAAG 
GATAGACCAG TGGTGCATGG CAAAGTGCAA TTTGACACAG TCCTTAAAAA TTATTCACAG 
TATCATACTA CAGTATGCCA GGTGTTTGAA AGTACCTGCA GAAATCACAA GCATGAGAAG 
AATACAGGCT TATCAGGAGC TTGCATAACT GCAATGAAAA CTGAAACAAG AATCCTGGAT 
ACTGTGTGCA TTACAGAAGA CTGTGGCCCA CAAAACAGAC CTGCAAGTTT TTCACCTGGC 
AATGGCTCAG ATAAGGGCAG CTGTGAACTT CAAAACAAAA CGCAAAGAAG AATTCCTCAT 
TTTAATCAAG TCAACACCTC ACTTGATGAA GAAACCAAAA GCAGTGATTT GGATCTGGAG 
GACAATAAAA GAGGTAGATC TCTTAAGGGC AGCCAAAGCT CTTGTGCACA AAAGTCTTGG 
TTCCAGGTTT CTGGGTCAGG CTCTTCCAGC AGCTGGGAGG AAATAGATGA CCAAGGTGGC 
CTTGCAATAC CCAAAGAAAA AAGTGGTTTT GATCAGGATG TTATAGACAC AAGTTGTTCC 
ACAGCTTTCA CTGAAGATGC CAAAGAAGAG GGTCAAGGCT GCGCTGCTCC AGCGTTACCC 
ATGTGCTTTC AGAATCTTTC TGTAAGGGGG GATGTGAGCA CCCATTCTGC TGCTCCTCAT 
AGCCCAATAA GTTTGTCAGG TTGCCTGGTA AAGAGAGAGA TCTCCCCAGG CTCTAGAGAG 
AAGCCTTTGG AAGATAATGA AACTGACTTT CAGCTAGAGA CTACAGAAGA CACAAGTGAT 
GGAACCAACA ACATTAAAGA CCATCCAGGT ATCAACAAAA GACAAGTCTC TCATCCTTCA 
AGAGCTTCTC AGATGTCTTC CAACACCTCG CAGCCCATCC CTTCTCTGAG TTCAAAGACT 
GCTGTCGGAA ATCTTTCTTT AATTATAACT GAGAGTCATT CCTTTGAGAT GGTTGAGGTT 
GACCGTGAAC TGGAAACTGC AAGTGGCATA AAAGGTGCAA GTGATGTCTT AAGTGCCAAC 
TGTGGTGCTG ACAAAAATTG CTCAAGTGGT GTGAGCATAA AAATGTCATC CACAGTAGAA 
GAAAGCAAAT CCTTTAAAGT TGACTCAAAA GCAGAGGCTG CAGATGAGAA TAAGGACATA 
CGTGCAGATG CATTTACCTC TACAGATGAA AAAGTTCCTG CAGGTTCTGC AAACTGTTCA 
GAAGAAGCTA ATCGTCTTTC TGTCAGTAAG GAAGCCTCTC TAGTATCAGA TAAAAATATG 
TCTATTGAAA TGCTTGAAGT TGATCCTGAA GCAGAAACAG CTGATATGTT AGAAGATACT 
CCTGGTAATA TTGAAAGTCG TTCATCTCCT TTCACAAATG TACGTCTTCA CAGCCAACAG 
AGAACTGAGC AGGGGAATGG CAAGACAGAT GGTTCAACTC CAGTTTCAAA CGTGGACAGG 
TTGGAAGAGC TGGAATTCGG CAATTCAGAA ACAACAGAGG AAGACGAGGG CGATAGACTA 
CTGGGCCTTG CTCTCAACAG TAGTAACAGC TCCACCTCTT CCTTTAGGTC GTGGTACAAG 
TCACCATATT TTTCTAGCAG TTTCTCTGAA GTGGAGAGCC CTTCCAGTTT AAATTCCAGT 
GGGAATTCCT TTGTGTTTGT TACTGGAAGG AGCGAAGAAG AGATACACAA AGCTCGCATT 
TTTAATTATG AAGATTACGA AAAACTCTTC AAAGGAGTAA GTCATAGTTG GTTACTGGAA 
CGACTCTGTA ATACTGGAAT ATTTAAGCCT AAGAGACTCC AAAAAGTTTA CACTGCTCTC 
CTTTTAAAAT ATTCGAAGAC ATCTGACAAG TGGACAGCCC AGGAAACACT TGTGCATATT 
GGAGAGTATC TCTCTCTAAA CAAGGCAGGC AAGCAAAGGA ATGCATTCTG GATCCAGTTT 
CTACATCAGG AAGAGACACT GGGAAGGTAT GTTGGAAAAG AGTATAAAGT TCAAAAGGAG 
CTCTCCTTCC ATTTCAACGA CGTAGAACGC CAAATGACTG CACAGCACTA TGTGACACAG 
TTCAACAAGA GACTGTACGA GCAGAAAGTT CCTACACAAA TATTCTATAT ACCTGCGTCA 
GTACTTTTGG TTTTGGAAGA TATGGAAATA AAAGGATGTA TAAGTGTGGA GCCATACATG 
CTTGGAGATT TTGTAAAGTT AACCAACAAT ACAAAGGTAG CAAAAGAGGA ATATGAAGCA 
ACAACGTATG GACTGGCATT TGGACATTTC ACCTATGAAT TTTCTAAATA TAAAGAAGTT 
GTCGTTGATT TACAAGGTTG GGTGACAGGA GACGGAAAAG GACTAATTTA CCTAACAGAC 
CCTCAGATTC ACTCAGTGGA TCAAAGTAAA AAAGTGGGCA CAAACTTCGG ACCAAAAGGC 
ATTAACTACT TTTTTAACTA TCAGCATGTA GAGTGCAATG ACATCTGCCG TAGACTTTCA 
CTAACAAAAT CTTTGAGGCC AAAACCTCTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006011421.1
CDS	258..4070
Translation	MNNQEVVTILQECKQALDALSSGTSEPAEEDKKVYQRCEASLSDDLRTLLQEAKEMKWPFVPERWQYKQALCPEDKTNLKDMISAKLHELLVYLKASIVVKDCTTAAAVVFLIDRFLYWVDASSRLLQVAKGLHKLCPTTPIAPQVVIRQARVSVNSGKLLKAEYILSSLINNNGATGSWTYAKESDRVLVQSVSIQIRGQILLKLGMWYEAAELIWASIVGYFELPLPDKKGIATSLGILADIFVSMNDKDYDAFKNNPRVDLCLLKEFGHRLLSAAEAAKLAAVFSQYTSLFVLTSTNIRGTCLLSYSFSSDCPPEKKKHYLSEAKEAFEVSLLTKKDNEVVTSKQELHSFVKAAFCLTAAHKGLSSEKETLQEARQMCKAAMEKLYSYGTLSETVEKRTLAEEIMALVKKVKDRLQVRPFPNSDDRSYIPDSYKVCKDRPVVHGKVQFDTVLKNYSQYHTTVCQVFESTCRNHKHEKNTGLSGACITAMKTETRILDTVCITEDCGPQNRPASFSPGNGSDKGSCELQNKTQRRIPHFNQVNTSLDEETKSSDLDLEDNKRGRSLKGSQSSCAQKSWFQVSGSGSSSSWEEIDDQGGLAIPKEKSGFDQDVIDTSCSTAFTEDAKEEGQGCAAPALPMCFQNLSVRGDVSTHSAAPHSPISLSGCLVKREISPGSREKPLEDNETDFQLETTEDTSDGTNNIKDHPGINKRQVSHPSRASQMSSNTSQPIPSLSSKTAVGNLSLIITESHSFEMVEVDRELETASGIKGASDVLSANCGADKNCSSGVSIKMSSTVEESKSFKVDSKAEAADENKDIRADAFTSTDEKVPAGSANCSEEANRLSVSKEASLVSDKNMSIEMLEVDPEAETADMLEDTPGNIESRSSPFTNVRLHSQQRTEQGNGKTDGSTPVSNVDRLEELEFGNSETTEEDEGDRLLGLALNSSNSSTSSFRSWYKSPYFSSSFSEVESPSSLNSSGNSFVFVTGRSEEEIHKARIFNYEDYEKLFKGVSHSWLLERLCNTGIFKPKRLQKVYTALLLKYSKTSDKWTAQETLVHIGEYLSLNKAGKQRNAFWIQFLHQEETLGRYVGKEYKVQKELSFHFNDVERQMTAQHYVTQFNKRLYEQKVPTQIFYIPASVLLVLEDMEIKGCISVEPYMLGDFVKLTNNTKVAKEEYEATTYGLAFGHFTYEFSKYKEVVVDLQGWVTGDGKGLIYLTDPQIHSVDQSKKVGTNFGPKGINYFFNYQHVECNDICRRLSLTKSLRPKPL

Target ORF information:

RefSeq Version	XM_006011359.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae alpha-kinase 1 (ALPK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006011359.2

ORF Insert Sequence:

ATGAATAATC AAGAAGTTGT GACCATACTA CAGGAGTGCA AGCAAGCTTT GGACGCGCTC 
TCATCAGGGA CGTCAGAACC AGCCGAGGAG GACAAGAAAG TGTACCAACG GTGTGAAGCT 
TCACTCTCTG ATGATCTGAG GACCCTGCTT CAGGAGGCAA AGGAAATGAA GTGGCCCTTT 
GTGCCAGAGC GATGGCAATA CAAACAAGCC TTATGCCCAG AAGACAAAAC AAATCTGAAA 
GATATGATCA GTGCAAAGCT GCATGAGCTG CTGGTTTATT TGAAAGCCTC CATTGTGGTG 
AAGGATTGCA CTACAGCAGC TGCCGTTGTG TTTTTGATCG ACCGTTTTCT GTACTGGGTA 
GATGCTTCCA GCAGGCTGCT GCAGGTTGCT AAGGGCCTGC ACAAGCTTTG CCCTACAACA 
CCGATCGCGC CACAGGTGGT CATTCGACAG GCCCGCGTCT CTGTAAATTC AGGCAAGCTC 
TTGAAAGCTG AGTACATCCT CAGCAGCCTC ATCAACAACA ACGGTGCGAC AGGATCATGG 
ACATATGCCA AGGAAAGTGA CAGAGTCCTT GTGCAGTCTG TCAGCATTCA GATCAGAGGA 
CAGATTCTAC TAAAACTTGG AATGTGGTAT GAGGCTGCAG AGTTGATTTG GGCCTCGATA 
GTTGGATATT TCGAACTTCC TTTACCAGAT AAAAAGGGCA TAGCAACTTC TTTGGGCATT 
TTGGCTGACA TATTTGTGTC CATGAATGAT AAAGATTACG ACGCGTTTAA AAATAATCCT 
CGGGTTGACT TGTGTCTGCT TAAAGAGTTT GGGCATCGCC TGTTGTCAGC AGCAGAAGCA 
GCCAAGCTGG CTGCTGTGTT CAGCCAGTAC ACGTCGCTCT TTGTGCTCAC TAGTACGAAC 
ATTCGTGGGA CATGTTTATT ATCCTATAGT TTCTCAAGTG ATTGTCCACC AGAGAAGAAA 
AAACATTATC TCTCAGAAGC CAAGGAGGCC TTTGAAGTTA GCCTCCTCAC AAAGAAAGAT 
AATGAAGTAG TTACAAGCAA GCAGGAGCTT CACAGTTTTG TTAAGGCTGC ATTCTGCTTA 
ACGGCAGCAC ACAAGGGCCT CAGCAGTGAA AAAGAAACAC TCCAGGAAGC CAGACAGATG 
TGTAAAGCAG CAATGGAAAA ACTGTACTCC TACGGCACCT TGTCAGAAAC AGTGGAAAAG 
AGGACACTTG CCGAGGAGAT CATGGCGTTG GTGAAGAAAG TTAAAGATCG TTTGCAGGTC 
AGGCCTTTTC CAAATTCAGA TGACAGATCT TATATTCCCG ATAGCTATAA AGTTTGTAAG 
GATAGACCAG TGGTGCATGG CAAAGTGCAA TTTGACACAG TCCTTAAAAA TTATTCACAG 
TATCATACTA CAGTATGCCA GGTGTTTGAA AGTACCTGCA GAAATCACAA GCATGAGAAG 
AATACAGGCT TATCAGGAGC TTGCATAACT GCAATGAAAA CTGAAACAAG AATCCTGGAT 
ACTGTGTGCA TTACAGAAGA CTGTGGCCCA CAAAACAGAC CTGCAAGTTT TTCACCTGGC 
AATGGCTCAG ATAAGGGCAG CTGTGAACTT CAAAACAAAA CGCAAAGAAG AATTCCTCAT 
TTTAATCAAG TCAACACCTC ACTTGATGAA GAAACCAAAA GCAGTGATTT GGATCTGGAG 
GACAATAAAA GAGGTAGATC TCTTAAGGGC AGCCAAAGCT CTTGTGCACA AAAGTCTTGG 
TTCCAGGTTT CTGGGTCAGG CTCTTCCAGC AGCTGGGAGG AAATAGATGA CCAAGGTGGC 
CTTGCAATAC CCAAAGAAAA AAGTGGTTTT GATCAGGATG TTATAGACAC AAGTTGTTCC 
ACAGCTTTCA CTGAAGATGC CAAAGAAGAG GGTCAAGGCT GCGCTGCTCC AGCGTTACCC 
ATGTGCTTTC AGAATCTTTC TGTAAGGGGG GATGTGAGCA CCCATTCTGC TGCTCCTCAT 
AGCCCAATAA GTTTGTCAGG TTGCCTGGTA AAGAGAGAGA TCTCCCCAGG CTCTAGAGAG 
AAGCCTTTGG AAGATAATGA AACTGACTTT CAGCTAGAGA CTACAGAAGA CACAAGTGAT 
GGAACCAACA ACATTAAAGA CCATCCAGGT ATCAACAAAA GACAAGTCTC TCATCCTTCA 
AGAGCTTCTC AGATGTCTTC CAACACCTCG CAGCCCATCC CTTCTCTGAG TTCAAAGACT 
GCTGTCGGAA ATCTTTCTTT AATTATAACT GAGAGTCATT CCTTTGAGAT GGTTGAGGTT 
GACCGTGAAC TGGAAACTGC AAGTGGCATA AAAGGTGCAA GTGATGTCTT AAGTGCCAAC 
TGTGGTGCTG ACAAAAATTG CTCAAGTGGT GTGAGCATAA AAATGTCATC CACAGTAGAA 
GAAAGCAAAT CCTTTAAAGT TGACTCAAAA GCAGAGGCTG CAGATGAGAA TAAGGACATA 
CGTGCAGATG CATTTACCTC TACAGATGAA AAAGTTCCTG CAGGTTCTGC AAACTGTTCA 
GAAGAAGCTA ATCGTCTTTC TGTCAGTAAG GAAGCCTCTC TAGTATCAGA TAAAAATATG 
TCTATTGAAA TGCTTGAAGT TGATCCTGAA GCAGAAACAG CTGATATGTT AGAAGATACT 
CCTGGTAATA TTGAAAGTCG TTCATCTCCT TTCACAAATG TACGTCTTCA CAGCCAACAG 
AGAACTGAGC AGGGGAATGG CAAGACAGAT GGTTCAACTC CAGTTTCAAA CGTGGACAGG 
TTGGAAGAGC TGGAATTCGG CAATTCAGAA ACAACAGAGG AAGACGAGGG CGATAGACTA 
CTGGGCCTTG CTCTCAACAG TAGTAACAGC TCCACCTCTT CCTTTAGGTC GTGGTACAAG 
TCACCATATT TTTCTAGCAG TTTCTCTGAA GTGGAGAGCC CTTCCAGTTT AAATTCCAGT 
GGGAATTCCT TTGTGTTTGT TACTGGAAGG AGCGAAGAAG AGATACACAA AGCTCGCATT 
TTTAATTATG AAGATTACGA AAAACTCTTC AAAGGAGTAA GTCATAGTTG GTTACTGGAA 
CGACTCTGTA ATACTGGAAT ATTTAAGCCT AAGAGACTCC AAAAAGTTTA CACTGCTCTC 
CTTTTAAAAT ATTCGAAGAC ATCTGACAAG TGGACAGCCC AGGAAACACT TGTGCATATT 
GGAGAGTATC TCTCTCTAAA CAAGGCAGGC AAGCAAAGGA ATGCATTCTG GATCCAGTTT 
CTACATCAGG AAGAGACACT GGGAAGGTAT GTTGGAAAAG AGTATAAAGT TCAAAAGGAG 
CTCTCCTTCC ATTTCAACGA CGTAGAACGC CAAATGACTG CACAGCACTA TGTGACACAG 
TTCAACAAGA GACTGTACGA GCAGAAAGTT CCTACACAAA TATTCTATAT ACCTGCGTCA 
GTACTTTTGG TTTTGGAAGA TATGGAAATA AAAGGATGTA TAAGTGTGGA GCCATACATG 
CTTGGAGATT TTGTAAAGTT AACCAACAAT ACAAAGGTAG CAAAAGAGGA ATATGAAGCA 
ACAACGTATG GACTGGCATT TGGACATTTC ACCTATGAAT TTTCTAAATA TAAAGAAGTT 
GTCGTTGATT TACAAGGTTG GGTGACAGGA GACGGAAAAG GACTAATTTA CCTAACAGAC 
CCTCAGATTC ACTCAGTGGA TCAAAGTAAA AAAGTGGGCA CAAACTTCGG ACCAAAAGGC 
ATTAACTACT TTTTTAACTA TCAGCATGTA GAGTGCAATG ACATCTGCCG TAGACTTTCA 
CTAACAAAAT CTTTGAGGCC AAAACCTCTG TAA