PIAS4 cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIAS4
Entrez Gene ID	102350892
Full Name	protein inhibitor of activated STAT 4
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014490265.1	XP_014345751.1	E3 SUMO-protein ligase PIAS4

Pathways from BioSystems
Gene Ontology

The following PIAS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIAS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa29989	XM_014490265.1 Latest version!	Latimeria chalumnae protein inhibitor of activated STAT, 4 (PIAS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIAS4 ( XM_014490265.1 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_014345751.1 Latimeria chalumnae protein inhibitor of activated STAT, 4 (PIAS4), mRNA.

Price & Availability↑

CloneID	OLa29989
Clone ID Related Accession (Same CDS sequence)	XM_014490265.1
Accession Version	XM_014490265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	E3 SUMO-protein ligase PIAS4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819511.1) and transcript sequence (GAAA01057050.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 133 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCGGCGG AGCTGGCGGA GGCCGAGGAT ATGGTGTTGG GTTTCAGAGT TTCTGACCTA 
CAGACATTAT TGGGATCTTT TGGGGTCAGT AAAAGTGGAC TCAAACATGA GCTAGTTACC 
AGGGCCTTGA AGCTTGTCCA GTCGTCCTAC AGCCCCGAGC TGCAAAAGAA GATAAAGGAG 
CTTTATGAAT TCCGCGTTAG GAAGAACTCG GATCTACCGC AGCAGCTCAG AACCTCCGAA 
GTGCATTCCA TAAGCAGCAC TTTTGATCCT GCAAATCCTC TCACCGGGTC TGCCGGCACC 
ATTGACTACT CTGGGCAGCA CACGAAATAT GGCAAATACC TCAACGGACT GGGAAAGCCC 
ACCAGCAAGA ACACAAAGCC AGAAGTTCGG CTCGTCTCCC TTCCCTTTTA CGAAGTGTTA 
GATCAGTTGT TGAAGCCTAC GGAATTAGTT CCGCAAAGCA ATGAAAAGTT TCAGGAAAGC 
CCTTGCACCT TTGCACTGAC TCCGAGACAA GTGGAGCTAA TTAGAAATTC CAGGGAACTG 
CAGCCTGGTA TTAAATCTGT TCAGGTAGTG CTTCGAATTT GTTACGCGGA CAGTAGCTGT 
ACTCAAGAAG ATCAGTATCC TCCAAATATA GCTGTGAAGG TGAACCACAG TTACTGTACT 
GTCCCAGGTT ACTACCCCTC CACTAAACCT GGCGTAGAGC CAAAGCGGCC GTGTCGGCCG 
GTCAATCTCA CTCATCTGTT ACACATGTCT TCCACGACTA ACCGTATCAC AGTCACGTGG 
GGAAACTTTG GAAAGAAATA CTCAGTGGCT TTATATCTTG TCAGACAGCT AACGTCTGCC 
GAGTTGCTGC AGAAGTTGAA AACGATCGGA ATCAAACACC CAGAGCTCTG TAAAGCACTT 
ATTCGGGAAA AACTGCGTTT GGACCCAGAC AGTGAAATAA CCACAACTGG TCTTCGAGTT 
TCCCTTATGT GTCCACTTGT GAAAATGCGC TTGTCGGTAC CTTGCCGTGC GGAAACGTGT 
GCACACCTAC AGTGTTTTGA TGCGGTTTTC TACTTGCAAA TGAATGAGAA GAAGCCAACG 
TGGATGTGTC CTGTCTGTGA CAAGCCAGCA ACTTACGACC AGTTGACAAT AGATGGGTGT 
TCTCCGGAGG TTGATGGATT TATATCTGTT CCTGACGCCA ACGCTGTAAA TGGCAAAAGC 
AGCTGCGATG TGGTTGACTT GACAGTAGAC AGTTCGTCGG AGGACGATGA GGAACCCCCG 
CGGAAAAGAG GGCGCTGTGG CTATGACAAA GGGTTGGTTT CTGCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014345751.1
CDS	36..1346
Translation	MAAELAEAEDMVLGFRVSDLQTLLGSFGVSKSGLKHELVTRALKLVQSSYSPELQKKIKELYEFRVRKNSDLPQQLRTSEVHSISSTFDPANPLTGSAGTIDYSGQHTKYGKYLNGLGKPTSKNTKPEVRLVSLPFYEVLDQLLKPTELVPQSNEKFQESPCTFALTPRQVELIRNSRELQPGIKSVQVVLRICYADSSCTQEDQYPPNIAVKVNHSYCTVPGYYPSTKPGVEPKRPCRPVNLTHLLHMSSTTNRITVTWGNFGKKYSVALYLVRQLTSAELLQKLKTIGIKHPELCKALIREKLRLDPDSEITTTGLRVSLMCPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPATYDQLTIDGCSPEVDGFISVPDANAVNGKSSCDVVDLTVDSSSEDDEEPPRKRGRCGYDKGLVSAY

Target ORF information:

RefSeq Version	XM_014490265.1
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae protein inhibitor of activated STAT, 4 (PIAS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014490265.1

ORF Insert Sequence:

ATGGCGGCGG AGCTGGCGGA GGCCGAGGAT ATGGTGTTGG GTTTCAGAGT TTCTGACCTA 
CAGACATTAT TGGGATCTTT TGGGGTCAGT AAAAGTGGAC TCAAACATGA GCTAGTTACC 
AGGGCCTTGA AGCTTGTCCA GTCGTCCTAC AGCCCCGAGC TGCAAAAGAA GATAAAGGAG 
CTTTATGAAT TCCGCGTTAG GAAGAACTCG GATCTACCGC AGCAGCTCAG AACCTCCGAA 
GTGCATTCCA TAAGCAGCAC TTTTGATCCT GCAAATCCTC TCACCGGGTC TGCCGGCACC 
ATTGACTACT CTGGGCAGCA CACGAAATAT GGCAAATACC TCAACGGACT GGGAAAGCCC 
ACCAGCAAGA ACACAAAGCC AGAAGTTCGG CTCGTCTCCC TTCCCTTTTA CGAAGTGTTA 
GATCAGTTGT TGAAGCCTAC GGAATTAGTT CCGCAAAGCA ATGAAAAGTT TCAGGAAAGC 
CCTTGCACCT TTGCACTGAC TCCGAGACAA GTGGAGCTAA TTAGAAATTC CAGGGAACTG 
CAGCCTGGTA TTAAATCTGT TCAGGTAGTG CTTCGAATTT GTTACGCGGA CAGTAGCTGT 
ACTCAAGAAG ATCAGTATCC TCCAAATATA GCTGTGAAGG TGAACCACAG TTACTGTACT 
GTCCCAGGTT ACTACCCCTC CACTAAACCT GGCGTAGAGC CAAAGCGGCC GTGTCGGCCG 
GTCAATCTCA CTCATCTGTT ACACATGTCT TCCACGACTA ACCGTATCAC AGTCACGTGG 
GGAAACTTTG GAAAGAAATA CTCAGTGGCT TTATATCTTG TCAGACAGCT AACGTCTGCC 
GAGTTGCTGC AGAAGTTGAA AACGATCGGA ATCAAACACC CAGAGCTCTG TAAAGCACTT 
ATTCGGGAAA AACTGCGTTT GGACCCAGAC AGTGAAATAA CCACAACTGG TCTTCGAGTT 
TCCCTTATGT GTCCACTTGT GAAAATGCGC TTGTCGGTAC CTTGCCGTGC GGAAACGTGT 
GCACACCTAC AGTGTTTTGA TGCGGTTTTC TACTTGCAAA TGAATGAGAA GAAGCCAACG 
TGGATGTGTC CTGTCTGTGA CAAGCCAGCA ACTTACGACC AGTTGACAAT AGATGGGTGT 
TCTCCGGAGG TTGATGGATT TATATCTGTT CCTGACGCCA ACGCTGTAAA TGGCAAAAGC 
AGCTGCGATG TGGTTGACTT GACAGTAGAC AGTTCGTCGG AGGACGATGA GGAACCCCCG 
CGGAAAAGAG GGCGCTGTGG CTATGACAAA GGGTTGGTTT CTGCTTACTA A