map3k4 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k4
Entrez Gene ID	104940016
Full Name	mitogen-activated protein kinase kinase kinase 4
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019275673.2	XP_019131218.2	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_019275674.1	XP_019131219.1	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_019275675.1	XP_019131220.1	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_019275675.2	XP_019131220.2	mitogen-activated protein kinase kinase kinase 4 isoform X4
XM_019275673.1	XP_019131218.1	mitogen-activated protein kinase kinase kinase 4 isoform X2
XM_019275672.2	XP_019131217.2	mitogen-activated protein kinase kinase kinase 4 isoform X1
XM_019275674.2	XP_019131219.2	mitogen-activated protein kinase kinase kinase 4 isoform X3
XM_019275672.1	XP_019131217.1	mitogen-activated protein kinase kinase kinase 4 isoform X1

Pathways from BioSystems
Gene Ontology

The following map3k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa109667	XM_019275672.1 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa109668	XM_019275673.1 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa109669	XM_019275674.1 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa109670	XM_019275675.1 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa218133	XM_019275672.2 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa218134	XM_019275673.2 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa218135	XM_019275674.2 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OLa218136	XM_019275675.2 Latest version!	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k4 ( XM_019275672.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131217.1 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa109667
Clone ID Related Accession (Same CDS sequence)	XM_019275672.1
Accession Version	XM_019275672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCTCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGAG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCGCTA CTGACTCTGA CTGGGCTCAG TGGGAAGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCTAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGCTCTCG AAGTCTCCCC TGCGACCTCC GGAACCAGCT GTTCCCCAAC 
GGCCCTCGCC CCGTCCCTCA GGGCCCGGGG GAACAAAGCA ACACCAAAGC ACCCAGCAGC 
AGCCCCAATG ACGTCAGGGG ATCCAGTTTC CATGAGAATG ACCGCCTGTC GTCAGTTGCA 
GCAGAGTTGC ATTTTAAATC TCTGAGCCGC CACTCCAGCC CCACAGAGGA CAGAGAAGAA 
CCCTCTTATC CTAAGGGGGA TCCTAACAGC ACTGCACGCC GAAGCTGGGA GCTTCGCAAC 
TTTATCAGCC AGTCCAAGGA CACAGCAGCA AGGCAAAGCC CCATGGAGGC TGTCCGTCGC 
TCCATTCGCA AGTTCGAGGA CAAACGCTAT GCTGTGATGA AGCAGCGCAA CATCATCGGC 
CAAGTGTGTC ACACACCCAA ATCCTATGAC AATGTCATGC ATGTCGGGCT CAGGAAGGTG 
ACCTTTAAGT GGCAGAGAGG CAACAAGATA GGTGAGGGCC AGTATGGGAA AGTGTACACC 
TGCATCAATG TTGACACTGG AGAACTAATG GCCATGAAGG AGATCCGATT CCAGCCGAAT 
GATCACAAAA CAATCAAGGA GACTGCAGAT GAGCTGAAAA TATTTGAAGG CATTAAACAC 
CCAAACTTGG TGCGATACTT TGGAGTGGAG CTCCATCGGG AGGAGATGTA CATCTTCATG 
GAGTACTGTG ACGAGGGCAC CCTGGAGGAG GTGTCCAGAC TGGGGCTGCA GGAACATGTC 
ATCAGGCTCT ACAGTAAACA GATCACTACA GCCATCAATG TCCTCCATGA GCATGGCATT 
GTCCACCGCG ATATCAAGGG AGCCAACATC TTTTTGACAT CGTCTGGCCT GATTAAGCTG 
GGTGACTTTG GCTGTTCAGT GAAATTGAGG AACAACACAC ACACCATGCC AGGGGAAGTG 
AACAGCACGC TGGGAACAGC AGCATACATG GCACCTGAAG TCATCACCAG AGCAAAAGGG 
GAAGGTCACG GACGAGCGGC GGACATCTGG AGTTTGGGCT GTGTTCTCAT TGAGATGGTG 
ACTGGAAAGA GGCCCTGGCA CGAGTATGAG CACAACTTCC AGATCATGTA CAAAGTGGGA 
ATGGGCCATA AACCCCCCAT CCCAGAGAAA CTCAGCACAG AGGGGAAGGA CTTCCTTGGT 
CACTGTCTGG AGAGTGAACC CAAACGCCGC TGGACAGCTA GCATGCTGCT AGACCACCCG 
TTTGTCAAGG TTTGTACGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131217.1
CDS	302..5071
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGSFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWEDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSATDSDWAQWEGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275672.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275672.1

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCTCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGAG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCGCTA CTGACTCTGA CTGGGCTCAG TGGGAAGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCTAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGCTCTCG AAGTCTCCCC TGCGACCTCC GGAACCAGCT GTTCCCCAAC 
GGCCCTCGCC CCGTCCCTCA GGGCCCGGGG GAACAAAGCA ACACCAAAGC ACCCAGCAGC 
AGCCCCAATG ACGTCAGGGG ATCCAGTTTC CATGAGAATG ACCGCCTGTC GTCAGTTGCA 
GCAGAGTTGC ATTTTAAATC TCTGAGCCGC CACTCCAGCC CCACAGAGGA CAGAGAAGAA 
CCCTCTTATC CTAAGGGGGA TCCTAACAGC ACTGCACGCC GAAGCTGGGA GCTTCGCAAC 
TTTATCAGCC AGTCCAAGGA CACAGCAGCA AGGCAAAGCC CCATGGAGGC TGTCCGTCGC 
TCCATTCGCA AGTTCGAGGA CAAACGCTAT GCTGTGATGA AGCAGCGCAA CATCATCGGC 
CAAGTGTGTC ACACACCCAA ATCCTATGAC AATGTCATGC ATGTCGGGCT CAGGAAGGTG 
ACCTTTAAGT GGCAGAGAGG CAACAAGATA GGTGAGGGCC AGTATGGGAA AGTGTACACC 
TGCATCAATG TTGACACTGG AGAACTAATG GCCATGAAGG AGATCCGATT CCAGCCGAAT 
GATCACAAAA CAATCAAGGA GACTGCAGAT GAGCTGAAAA TATTTGAAGG CATTAAACAC 
CCAAACTTGG TGCGATACTT TGGAGTGGAG CTCCATCGGG AGGAGATGTA CATCTTCATG 
GAGTACTGTG ACGAGGGCAC CCTGGAGGAG GTGTCCAGAC TGGGGCTGCA GGAACATGTC 
ATCAGGCTCT ACAGTAAACA GATCACTACA GCCATCAATG TCCTCCATGA GCATGGCATT 
GTCCACCGCG ATATCAAGGG AGCCAACATC TTTTTGACAT CGTCTGGCCT GATTAAGCTG 
GGTGACTTTG GCTGTTCAGT GAAATTGAGG AACAACACAC ACACCATGCC AGGGGAAGTG 
AACAGCACGC TGGGAACAGC AGCATACATG GCACCTGAAG TCATCACCAG AGCAAAAGGG 
GAAGGTCACG GACGAGCGGC GGACATCTGG AGTTTGGGCT GTGTTCTCAT TGAGATGGTG 
ACTGGAAAGA GGCCCTGGCA CGAGTATGAG CACAACTTCC AGATCATGTA CAAAGTGGGA 
ATGGGCCATA AACCCCCCAT CCCAGAGAAA CTCAGCACAG AGGGGAAGGA CTTCCTTGGT 
CACTGTCTGG AGAGTGAACC CAAACGCCGC TGGACAGCTA GCATGCTGCT AGACCACCCG 
TTTGTCAAGG TTTGTACGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275673.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131218.1 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa109668
Clone ID Related Accession (Same CDS sequence)	XM_019275673.1
Accession Version	XM_019275673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG ACCTTAGCCC ATCTGGCCTG 
AGAGAAAGTC CCAGGCCAGT GGAGACCCCT GAAGAGCACA GCTACAAGCA GGGCAAGAAG 
CAGAGGGCCA CACTGCGCTC CACGGAGAGA GACCACAAGA AGACCTTTGA AGGCTCCTTC 
ATGCTGGACC CACTCTCAAA GTCGACTCAT TTCGGTTCCC TCAATATGGA TCCCAGGAAG 
CATTACTTGA GCTTGGGTTG CAGCAGTGGC AAACTTCCTG TGTCTATGCC CCATATGGCT 
CGGACCCACC GCCAGACCTC CAGGACAGAC TGTCCTGCTG ACCGCCTCAA GTTCTTTGAA 
ACTCTGCGGC TGCTCCTCAA GCTCACGTCT ATGTCCTCCA AGAGGAAGGA GAAGGAGCAG 
AGAGGCCTGG AGAACATGGC CTTCATGGGT CACAACAACG AGGTCATTTG GTTGGAGCTG 
CAGGCTTGGC ATGCACGGCG CTCCACCGGC GACCAAGACC TTTTTCTTTT TACTGCCCGT 
CAAGCCATCC CTGACATCAT CAATGAGGTG CTGCACTTTA AGGTCAACTA TGCCAGCCTG 
AGAGGGGCCC AGTGTTCAGT CGAGGGGGAA TATCAGAGTG TCCCAGATCT AGTCTGTGGA 
GAGGAGAGAG AGCCTGCCGT AGCAGAGACC AACCACTGTG GAGTAGATCC CTGGGGCTTT 
AGTGCCTTCC CCTCATCAGC AATGAATGCA GCAGAGCCTC TGGGCTCTGG CGCTGTTTGC 
AGGGAGCATC TTCAGCGCCA GCGGCTGGCA TTTGAGCAGG TCAAACATGT TATAGAGTTG 
CTGGAGTCAG TGGAGGCTTT GTACCCCTCT TTGCAGGCCC TGCAGAAGGA CTATGAAAAG 
TATGCAGCAC GAGATTTCCA GGGCAGGGTG CAGGCGCTCT GTCTGTGGCT CAATATCACC 
CAGGACCTCA ACCAGAAGCT GCGTGTTATG GCAACTGTGC TGGGCCTCCA TGATCTATCT 
CGTATCGGGT GGCCTGTCTT TGAAATCCCC TCACCTCGCT GTTCCCGTGG CAATGAAGAA 
GAGGACAATG AGGAAGACGA GGAGAATGAC TCAACAGCCA CTTTTACAGC TGAAAGTGAT 
GGAGAGGACA GGGATGCTGA GGAGGAGGAG GAGGAGGAGG AGGTGGTAGT ATTGGGTCAC 
ATAGCAGAGG GAGAGTTGTC TCCCTCCCTG ACTCCTAAAT TTGCCCGATT GTTGTCAGAG 
GACGAGTTTC TCCCAACTGC TAACGCAGTA AGTGCAGAAG TGATTGCGGG AGGGGGAGTA 
TTCTGCCCCA CAGCCATCTA CAGACCGTTT GTGGATAAAG CTCTGAAGCA GATGGGGCTG 
CGTAAACTGA TCCTCAGACT GCACAAACTG ATGGACCGCT CTCTTCAGAG GTCCAGAGCA 
GCACTGCTCC GTCACACTCC TGCACTGGAG TTTGCAGATT TCCCTGACCC CATGCTGTAC 
AGTGATTACC TGCCAGAGCT ATCGCGCCAT GAGTCCTGCA GTGGTCCGGC ACATCCGGAG 
CTTGGGGCAG ACCAGGTTTC CTGGGAGGAT TTGCTGGACA TGGATCTCCC GTCCTTTCGG 
CCAGCGTTCC TTGTGCTGTG CAGAGTCCTT CTAAACGTCA TTCACGAATG CCTTAAACTG 
CGACTTGAAC AGAGGCCTGC TGGGGAGCCT TCACTGCTCA GCATCAAACA GTTGGTGCGT 
GAGTGTAAGG AGGTTCTTAA AGGTGGTCTT CTGATGAAGC AGTATTATCA GTTCATGCTG 
CGAGGCGTGG TGACTGATGC TCAGGGCTTG CAGACGAATG CCAACATTGA TGAGTTTGAA 
GAGGATCTTC ACAAGATGCT GGTGGTCTAC TTTGATTACA TGCACAGTTG GATCCAGATG 
TTGCAGCAGC TGCCTCAGGC CTCTCACAGC TTAAAGAACC TGTTGGAGGA GGAGTGGAAC 
TTCACCAAGG TCATCACTCC TTACATTCGA GGAGGGGAGG CCCAGTCCGG GAAGCTTTTT 
TGTGACATCG CTGGGATGCT GCTTAAATCC ACTGGAGAGT TCCTTGATGC CGGCCTGCAA 
AAGAGCGGTA ATGAATTCTG GGAAACTGCA GATGACAGCA CCGCCTCAGA TGAGATCAGG 
CGGTCAGTTA TCGAGACTAG TCGGTCGCTC AAAGAGCTGT TCCACGAGGC CAGGGAACGA 
GCGTCCAAGG CTCTCGGCTT TGCCAAGATG CTTCGCAAGG ACTTGGAAGT TGCTGCAGAT 
TTCAGCATCA CTAATGGGGT GACTTGTCTC CTGGAGGCAC TGAAGAAGAG AAACTATGTC 
AAGGTTCAGA TTCCAGGCTT GGAGGAGCTG CAGGTTTTTG TCCCTTGTGG CTTGATGGGT 
CAGCGGCCTC TCATCCTGCA GCTTCTCAAT GCTGCTGCTG GCAAAGACTG CTCCAAGGAG 
CCTGATGAAA TTGCAGAGGA CGAGGCCTAC CTGCTTATGA GTAAACACGG AGCTGGGGAT 
TCCGCTACTG ACTCTGACTG GGCTCAGTGG GAAGGAGAGC TGCTCAAACT GGTTCCCCAG 
ATGGAAACTG TTGACACTTT AAGGGCCATG AAGGTAGAGA ATGTGCTCTT GATTGTGATG 
CAGTCTGCTC ACCTGGTGGC CCAGCGAAAG GCCTTTCAGC AGTCCATGGA GGATGTGCTC 
ACTCTTAGCC GGGAGCAGAC ATCTAGTCAG CCACTCATTG CGAGTGCGCT GGAGGAACTC 
AAGGATGAGG CACTACAGCT ATGCATAAAG ATCAGCACTG CCATTGACCG AGTGGAGTTC 
ATGTTCACCA CAGAGTTTGA GGCAGAGGTG GAGGAGTCTG AGTCAGCCAC TCTGCATCAG 
TACTACAGGG AGGCAATGAT ACAGGGCTAC AATTTTGCCT TCGAGTACCA CAAGGAGGTG 
GTGCGGCTAA TGTCAGGGGA GTTCAGACAA AGGATCGGGG AGCGCTACAT TGCTTTTGCC 
CGCAAATGGA TGACCTATGT CCTGACCAAA TGTGAGAGTG GCAGGGGCAC CAAGCCCAGG 
TGGGCGACTC AGGGGTTTGA CTTTCTTCAG GCTATCGAAC CTGGCTTTAT ATCAGCATTG 
CCAGAGGATG ACTTCTTGAA TTTGCAAGCT TTGATGAATG AATGTATTGG ACATGTGATT 
GGAAAACCTC ATAGCCCAGT CACCGGCCTG TACATAGCTC CTAGAAACAG TCCTCGACCT 
GTAAAGGTGC CTCGCTGCCA TAGTGACCCA CCAAACCCCA ACCTGTTCAT CCCTAATGCT 
GAAGGTTTCA GCTCTCGAAG TCTCCCCTGC GACCTCCGGA ACCAGCTGTT CCCCAACGGC 
CCTCGCCCCG TCCCTCAGGG CCCGGGGGAA CAAAGCAACA CCAAAGCACC CAGCAGCAGC 
CCCAATGACG TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC AGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACTTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131218.1
CDS	302..5068
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKDLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGSFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWEDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSATDSDWAQWEGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275673.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275673.1

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG ACCTTAGCCC ATCTGGCCTG 
AGAGAAAGTC CCAGGCCAGT GGAGACCCCT GAAGAGCACA GCTACAAGCA GGGCAAGAAG 
CAGAGGGCCA CACTGCGCTC CACGGAGAGA GACCACAAGA AGACCTTTGA AGGCTCCTTC 
ATGCTGGACC CACTCTCAAA GTCGACTCAT TTCGGTTCCC TCAATATGGA TCCCAGGAAG 
CATTACTTGA GCTTGGGTTG CAGCAGTGGC AAACTTCCTG TGTCTATGCC CCATATGGCT 
CGGACCCACC GCCAGACCTC CAGGACAGAC TGTCCTGCTG ACCGCCTCAA GTTCTTTGAA 
ACTCTGCGGC TGCTCCTCAA GCTCACGTCT ATGTCCTCCA AGAGGAAGGA GAAGGAGCAG 
AGAGGCCTGG AGAACATGGC CTTCATGGGT CACAACAACG AGGTCATTTG GTTGGAGCTG 
CAGGCTTGGC ATGCACGGCG CTCCACCGGC GACCAAGACC TTTTTCTTTT TACTGCCCGT 
CAAGCCATCC CTGACATCAT CAATGAGGTG CTGCACTTTA AGGTCAACTA TGCCAGCCTG 
AGAGGGGCCC AGTGTTCAGT CGAGGGGGAA TATCAGAGTG TCCCAGATCT AGTCTGTGGA 
GAGGAGAGAG AGCCTGCCGT AGCAGAGACC AACCACTGTG GAGTAGATCC CTGGGGCTTT 
AGTGCCTTCC CCTCATCAGC AATGAATGCA GCAGAGCCTC TGGGCTCTGG CGCTGTTTGC 
AGGGAGCATC TTCAGCGCCA GCGGCTGGCA TTTGAGCAGG TCAAACATGT TATAGAGTTG 
CTGGAGTCAG TGGAGGCTTT GTACCCCTCT TTGCAGGCCC TGCAGAAGGA CTATGAAAAG 
TATGCAGCAC GAGATTTCCA GGGCAGGGTG CAGGCGCTCT GTCTGTGGCT CAATATCACC 
CAGGACCTCA ACCAGAAGCT GCGTGTTATG GCAACTGTGC TGGGCCTCCA TGATCTATCT 
CGTATCGGGT GGCCTGTCTT TGAAATCCCC TCACCTCGCT GTTCCCGTGG CAATGAAGAA 
GAGGACAATG AGGAAGACGA GGAGAATGAC TCAACAGCCA CTTTTACAGC TGAAAGTGAT 
GGAGAGGACA GGGATGCTGA GGAGGAGGAG GAGGAGGAGG AGGTGGTAGT ATTGGGTCAC 
ATAGCAGAGG GAGAGTTGTC TCCCTCCCTG ACTCCTAAAT TTGCCCGATT GTTGTCAGAG 
GACGAGTTTC TCCCAACTGC TAACGCAGTA AGTGCAGAAG TGATTGCGGG AGGGGGAGTA 
TTCTGCCCCA CAGCCATCTA CAGACCGTTT GTGGATAAAG CTCTGAAGCA GATGGGGCTG 
CGTAAACTGA TCCTCAGACT GCACAAACTG ATGGACCGCT CTCTTCAGAG GTCCAGAGCA 
GCACTGCTCC GTCACACTCC TGCACTGGAG TTTGCAGATT TCCCTGACCC CATGCTGTAC 
AGTGATTACC TGCCAGAGCT ATCGCGCCAT GAGTCCTGCA GTGGTCCGGC ACATCCGGAG 
CTTGGGGCAG ACCAGGTTTC CTGGGAGGAT TTGCTGGACA TGGATCTCCC GTCCTTTCGG 
CCAGCGTTCC TTGTGCTGTG CAGAGTCCTT CTAAACGTCA TTCACGAATG CCTTAAACTG 
CGACTTGAAC AGAGGCCTGC TGGGGAGCCT TCACTGCTCA GCATCAAACA GTTGGTGCGT 
GAGTGTAAGG AGGTTCTTAA AGGTGGTCTT CTGATGAAGC AGTATTATCA GTTCATGCTG 
CGAGGCGTGG TGACTGATGC TCAGGGCTTG CAGACGAATG CCAACATTGA TGAGTTTGAA 
GAGGATCTTC ACAAGATGCT GGTGGTCTAC TTTGATTACA TGCACAGTTG GATCCAGATG 
TTGCAGCAGC TGCCTCAGGC CTCTCACAGC TTAAAGAACC TGTTGGAGGA GGAGTGGAAC 
TTCACCAAGG TCATCACTCC TTACATTCGA GGAGGGGAGG CCCAGTCCGG GAAGCTTTTT 
TGTGACATCG CTGGGATGCT GCTTAAATCC ACTGGAGAGT TCCTTGATGC CGGCCTGCAA 
AAGAGCGGTA ATGAATTCTG GGAAACTGCA GATGACAGCA CCGCCTCAGA TGAGATCAGG 
CGGTCAGTTA TCGAGACTAG TCGGTCGCTC AAAGAGCTGT TCCACGAGGC CAGGGAACGA 
GCGTCCAAGG CTCTCGGCTT TGCCAAGATG CTTCGCAAGG ACTTGGAAGT TGCTGCAGAT 
TTCAGCATCA CTAATGGGGT GACTTGTCTC CTGGAGGCAC TGAAGAAGAG AAACTATGTC 
AAGGTTCAGA TTCCAGGCTT GGAGGAGCTG CAGGTTTTTG TCCCTTGTGG CTTGATGGGT 
CAGCGGCCTC TCATCCTGCA GCTTCTCAAT GCTGCTGCTG GCAAAGACTG CTCCAAGGAG 
CCTGATGAAA TTGCAGAGGA CGAGGCCTAC CTGCTTATGA GTAAACACGG AGCTGGGGAT 
TCCGCTACTG ACTCTGACTG GGCTCAGTGG GAAGGAGAGC TGCTCAAACT GGTTCCCCAG 
ATGGAAACTG TTGACACTTT AAGGGCCATG AAGGTAGAGA ATGTGCTCTT GATTGTGATG 
CAGTCTGCTC ACCTGGTGGC CCAGCGAAAG GCCTTTCAGC AGTCCATGGA GGATGTGCTC 
ACTCTTAGCC GGGAGCAGAC ATCTAGTCAG CCACTCATTG CGAGTGCGCT GGAGGAACTC 
AAGGATGAGG CACTACAGCT ATGCATAAAG ATCAGCACTG CCATTGACCG AGTGGAGTTC 
ATGTTCACCA CAGAGTTTGA GGCAGAGGTG GAGGAGTCTG AGTCAGCCAC TCTGCATCAG 
TACTACAGGG AGGCAATGAT ACAGGGCTAC AATTTTGCCT TCGAGTACCA CAAGGAGGTG 
GTGCGGCTAA TGTCAGGGGA GTTCAGACAA AGGATCGGGG AGCGCTACAT TGCTTTTGCC 
CGCAAATGGA TGACCTATGT CCTGACCAAA TGTGAGAGTG GCAGGGGCAC CAAGCCCAGG 
TGGGCGACTC AGGGGTTTGA CTTTCTTCAG GCTATCGAAC CTGGCTTTAT ATCAGCATTG 
CCAGAGGATG ACTTCTTGAA TTTGCAAGCT TTGATGAATG AATGTATTGG ACATGTGATT 
GGAAAACCTC ATAGCCCAGT CACCGGCCTG TACATAGCTC CTAGAAACAG TCCTCGACCT 
GTAAAGGTGC CTCGCTGCCA TAGTGACCCA CCAAACCCCA ACCTGTTCAT CCCTAATGCT 
GAAGGTTTCA GCTCTCGAAG TCTCCCCTGC GACCTCCGGA ACCAGCTGTT CCCCAACGGC 
CCTCGCCCCG TCCCTCAGGG CCCGGGGGAA CAAAGCAACA CCAAAGCACC CAGCAGCAGC 
CCCAATGACG TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC AGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACTTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275674.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131219.1 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

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CloneID	OLa109669
Clone ID Related Accession (Same CDS sequence)	XM_019275674.1
Accession Version	XM_019275674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGC AGGCCAAGCC AGTGCAAGAT GCATCCCAAC AGAACTCTGC GGTGGGTGAA 
TCCTGGGACT CTCCCAGCGA GGAAGAGGAA GCTCTGTATG GCACTTCACC TCCTTATAAC 
CCCCGTCAGA TGAAACGCAT GTCTGGCAAA CATCAAAGGA GCCAAGCCAG GAGTGCTGGT 
CGGTCTCCTA ACAAGGCAGA CCTTAGCCCA TCTGGCCTGA GAGAAAGTCC CAGGCCAGTG 
GAGACCCCTG AAGAGCACAG CTACAAGCAG GGCAAGAAGC AGAGGGCCAC ACTGCGCTCC 
ACGGAGAGAG ACCACAAGAA GACCTTTGAA GGCTCCTTCA TGCTGGACCC ACTCTCAAAG 
TCGACTCATT TCGGTTCCCT CAATATGGAT CCCAGGAAGC ATTACTTGAG CTTGGGTTGC 
AGCAGTGGCA AACTTCCTGT GTCTATGCCC CATATGGCTC GGACCCACCG CCAGACCTCC 
AGGACAGACT GTCCTGCTGA CCGCCTCAAG TTCTTTGAAA CTCTGCGGCT GCTCCTCAAG 
CTCACGTCTA TGTCCTCCAA GAGGAAGGAG AAGGAGCAGA GAGGCCTGGA GAACATGGCC 
TTCATGGGTC ACAACAACGA GGTCATTTGG TTGGAGCTGC AGGCTTGGCA TGCACGGCGC 
TCCACCGGCG ACCAAGACCT TTTTCTTTTT ACTGCCCGTC AAGCCATCCC TGACATCATC 
AATGAGGTGC TGCACTTTAA GGTCAACTAT GCCAGCCTGA GAGGGGCCCA GTGTTCAGTC 
GAGGGGGAAT ATCAGAGTGT CCCAGATCTA GTCTGTGGAG AGGAGAGAGA GCCTGCCGTA 
GCAGAGACCA ACCACTGTGG AGTAGATCCC TGGGGCTTTA GTGCCTTCCC CTCATCAGCA 
ATGAATGCAG CAGAGCCTCT GGGCTCTGGC GCTGTTTGCA GGGAGCATCT TCAGCGCCAG 
CGGCTGGCAT TTGAGCAGGT CAAACATGTT ATAGAGTTGC TGGAGTCAGT GGAGGCTTTG 
TACCCCTCTT TGCAGGCCCT GCAGAAGGAC TATGAAAAGT ATGCAGCACG AGATTTCCAG 
GGCAGGGTGC AGGCGCTCTG TCTGTGGCTC AATATCACCC AGGACCTCAA CCAGAAGCTG 
CGTGTTATGG CAACTGTGCT GGGCCTCCAT GATCTATCTC GTATCGGGTG GCCTGTCTTT 
GAAATCCCCT CACCTCGCTG TTCCCGTGGC AATGAAGAAG AGGACAATGA GGAAGACGAG 
GAGAATGACT CAACAGCCAC TTTTACAGCT GAAAGTGATG GAGAGGACAG GGATGCTGAG 
GAGGAGGAGG AGGAGGAGGA GGTGGTAGTA TTGGGTCACA TAGCAGAGGG AGAGTTGTCT 
CCCTCCCTGA CTCCTAAATT TGCCCGATTG TTGTCAGAGG ACGAGTTTCT CCCAACTGCT 
AACGCAGTAA GTGCAGAAGT GATTGCGGGA GGGGGAGTAT TCTGCCCCAC AGCCATCTAC 
AGACCGTTTG TGGATAAAGC TCTGAAGCAG ATGGGGCTGC GTAAACTGAT CCTCAGACTG 
CACAAACTGA TGGACCGCTC TCTTCAGAGG TCCAGAGCAG CACTGCTCCG TCACACTCCT 
GCACTGGAGT TTGCAGATTT CCCTGACCCC ATGCTGTACA GTGATTACCT GCCAGAGCTA 
TCGCGCCATG AGTCCTGCAG TGGTCCGGCA CATCCGGAGC TTGGGGCAGA CCAGGTTTCC 
TGGGAGGATT TGCTGGACAT GGATCTCCCG TCCTTTCGGC CAGCGTTCCT TGTGCTGTGC 
AGAGTCCTTC TAAACGTCAT TCACGAATGC CTTAAACTGC GACTTGAACA GAGGCCTGCT 
GGGGAGCCTT CACTGCTCAG CATCAAACAG TTGGTGCGTG AGTGTAAGGA GGTTCTTAAA 
GGTGGTCTTC TGATGAAGCA GTATTATCAG TTCATGCTGC GAGGCGTGGT GACTGATGCT 
CAGGGCTTGC AGACGAATGC CAACATTGAT GAGTTTGAAG AGGATCTTCA CAAGATGCTG 
GTGGTCTACT TTGATTACAT GCACAGTTGG ATCCAGATGT TGCAGCAGCT GCCTCAGGCC 
TCTCACAGCT TAAAGAACCT GTTGGAGGAG GAGTGGAACT TCACCAAGGT CATCACTCCT 
TACATTCGAG GAGGGGAGGC CCAGTCCGGG AAGCTTTTTT GTGACATCGC TGGGATGCTG 
CTTAAATCCA CTGGAGAGTT CCTTGATGCC GGCCTGCAAA AGAGCGGTAA TGAATTCTGG 
GAAACTGCAG ATGACAGCAC CGCCTCAGAT GAGATCAGGC GGTCAGTTAT CGAGACTAGT 
CGGTCGCTCA AAGAGCTGTT CCACGAGGCC AGGGAACGAG CGTCCAAGGC TCTCGGCTTT 
GCCAAGATGC TTCGCAAGGA CTTGGAAGTT GCTGCAGATT TCAGCATCAC TAATGGGGTG 
ACTTGTCTCC TGGAGGCACT GAAGAAGAGA AACTATGTCA AGGTTCAGAT TCCAGGCTTG 
GAGGAGCTGC AGGTTTTTGT CCCTTGTGGC TTGATGGGTC AGCGGCCTCT CATCCTGCAG 
CTTCTCAATG CTGCTGCTGG CAAAGACTGC TCCAAGGAGC CTGATGAAAT TGCAGAGGAC 
GAGGCCTACC TGCTTATGAG TAAACACGGA GCTGGGGATT CCGCTACTGA CTCTGACTGG 
GCTCAGTGGG AAGGAGAGCT GCTCAAACTG GTTCCCCAGA TGGAAACTGT TGACACTTTA 
AGGGCCATGA AGGTAGAGAA TGTGCTCTTG ATTGTGATGC AGTCTGCTCA CCTGGTGGCC 
CAGCGAAAGG CCTTTCAGCA GTCCATGGAG GATGTGCTCA CTCTTAGCCG GGAGCAGACA 
TCTAGTCAGC CACTCATTGC GAGTGCGCTG GAGGAACTCA AGGATGAGGC ACTACAGCTA 
TGCATAAAGA TCAGCACTGC CATTGACCGA GTGGAGTTCA TGTTCACCAC AGAGTTTGAG 
GCAGAGGTGG AGGAGTCTGA GTCAGCCACT CTGCATCAGT ACTACAGGGA GGCAATGATA 
CAGGGCTACA ATTTTGCCTT CGAGTACCAC AAGGAGGTGG TGCGGCTAAT GTCAGGGGAG 
TTCAGACAAA GGATCGGGGA GCGCTACATT GCTTTTGCCC GCAAATGGAT GACCTATGTC 
CTGACCAAAT GTGAGAGTGG CAGGGGCACC AAGCCCAGGT GGGCGACTCA GGGGTTTGAC 
TTTCTTCAGG CTATCGAACC TGGCTTTATA TCAGCATTGC CAGAGGATGA CTTCTTGAAT 
TTGCAAGCTT TGATGAATGA ATGTATTGGA CATGTGATTG GAAAACCTCA TAGCCCAGTC 
ACCGGCCTGT ACATAGCTCC TAGAAACAGT CCTCGACCTG TAAAGGTGCC TCGCTGCCAT 
AGTGACCCAC CAAACCCCAA CCTGTTCATC CCTAATGCTG AAGGTTTCAG CTCTCGAAGT 
CTCCCCTGCG ACCTCCGGAA CCAGCTGTTC CCCAACGGCC CTCGCCCCGT CCCTCAGGGC 
CCGGGGGAAC AAAGCAACAC CAAAGCACCC AGCAGCAGCC CCAATGACGT CAGGGGATCC 
AGTTTCCATG AGAATGACCG CCTGTCGTCA GTTGCAGCAG AGTTGCATTT TAAATCTCTG 
AGCCGCCACT CCAGCCCCAC AGAGGACAGA GAAGAACCCT CTTATCCTAA GGGGGATCCT 
AACAGCACTG CACGCCGAAG CTGGGAGCTT CGCAACTTTA TCAGCCAGTC CAAGGACACA 
GCAGCAAGGC AAAGCCCCAT GGAGGCTGTC CGTCGCTCCA TTCGCAAGTT CGAGGACAAA 
CGCTATGCTG TGATGAAGCA GCGCAACATC ATCGGCCAAG TGTGTCACAC ACCCAAATCC 
TATGACAATG TCATGCATGT CGGGCTCAGG AAGGTGACCT TTAAGTGGCA GAGAGGCAAC 
AAGATAGGTG AGGGCCAGTA TGGGAAAGTG TACACCTGCA TCAATGTTGA CACTGGAGAA 
CTAATGGCCA TGAAGGAGAT CCGATTCCAG CCGAATGATC ACAAAACAAT CAAGGAGACT 
GCAGATGAGC TGAAAATATT TGAAGGCATT AAACACCCAA ACTTGGTGCG ATACTTTGGA 
GTGGAGCTCC ATCGGGAGGA GATGTACATC TTCATGGAGT ACTGTGACGA GGGCACCCTG 
GAGGAGGTGT CCAGACTGGG GCTGCAGGAA CATGTCATCA GGCTCTACAG TAAACAGATC 
ACTACAGCCA TCAATGTCCT CCATGAGCAT GGCATTGTCC ACCGCGATAT CAAGGGAGCC 
AACATCTTTT TGACATCGTC TGGCCTGATT AAGCTGGGTG ACTTTGGCTG TTCAGTGAAA 
TTGAGGAACA ACACACACAC CATGCCAGGG GAAGTGAACA GCACGCTGGG AACAGCAGCA 
TACATGGCAC CTGAAGTCAT CACCAGAGCA AAAGGGGAAG GTCACGGACG AGCGGCGGAC 
ATCTGGAGTT TGGGCTGTGT TCTCATTGAG ATGGTGACTG GAAAGAGGCC CTGGCACGAG 
TATGAGCACA ACTTCCAGAT CATGTACAAA GTGGGAATGG GCCATAAACC CCCCATCCCA 
GAGAAACTCA GCACAGAGGG GAAGGACTTC CTTGGTCACT GTCTGGAGAG TGAACCCAAA 
CGCCGCTGGA CAGCTAGCAT GCTGCTAGAC CACCCGTTTG TCAAGGTTTG TACGGATGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131219.1
CDS	273..5018
Translation	MIRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGSFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWEDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSATDSDWAQWEGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275674.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275674.1

ORF Insert Sequence:

ATGATCAGGC AGGCCAAGCC AGTGCAAGAT GCATCCCAAC AGAACTCTGC GGTGGGTGAA 
TCCTGGGACT CTCCCAGCGA GGAAGAGGAA GCTCTGTATG GCACTTCACC TCCTTATAAC 
CCCCGTCAGA TGAAACGCAT GTCTGGCAAA CATCAAAGGA GCCAAGCCAG GAGTGCTGGT 
CGGTCTCCTA ACAAGGCAGA CCTTAGCCCA TCTGGCCTGA GAGAAAGTCC CAGGCCAGTG 
GAGACCCCTG AAGAGCACAG CTACAAGCAG GGCAAGAAGC AGAGGGCCAC ACTGCGCTCC 
ACGGAGAGAG ACCACAAGAA GACCTTTGAA GGCTCCTTCA TGCTGGACCC ACTCTCAAAG 
TCGACTCATT TCGGTTCCCT CAATATGGAT CCCAGGAAGC ATTACTTGAG CTTGGGTTGC 
AGCAGTGGCA AACTTCCTGT GTCTATGCCC CATATGGCTC GGACCCACCG CCAGACCTCC 
AGGACAGACT GTCCTGCTGA CCGCCTCAAG TTCTTTGAAA CTCTGCGGCT GCTCCTCAAG 
CTCACGTCTA TGTCCTCCAA GAGGAAGGAG AAGGAGCAGA GAGGCCTGGA GAACATGGCC 
TTCATGGGTC ACAACAACGA GGTCATTTGG TTGGAGCTGC AGGCTTGGCA TGCACGGCGC 
TCCACCGGCG ACCAAGACCT TTTTCTTTTT ACTGCCCGTC AAGCCATCCC TGACATCATC 
AATGAGGTGC TGCACTTTAA GGTCAACTAT GCCAGCCTGA GAGGGGCCCA GTGTTCAGTC 
GAGGGGGAAT ATCAGAGTGT CCCAGATCTA GTCTGTGGAG AGGAGAGAGA GCCTGCCGTA 
GCAGAGACCA ACCACTGTGG AGTAGATCCC TGGGGCTTTA GTGCCTTCCC CTCATCAGCA 
ATGAATGCAG CAGAGCCTCT GGGCTCTGGC GCTGTTTGCA GGGAGCATCT TCAGCGCCAG 
CGGCTGGCAT TTGAGCAGGT CAAACATGTT ATAGAGTTGC TGGAGTCAGT GGAGGCTTTG 
TACCCCTCTT TGCAGGCCCT GCAGAAGGAC TATGAAAAGT ATGCAGCACG AGATTTCCAG 
GGCAGGGTGC AGGCGCTCTG TCTGTGGCTC AATATCACCC AGGACCTCAA CCAGAAGCTG 
CGTGTTATGG CAACTGTGCT GGGCCTCCAT GATCTATCTC GTATCGGGTG GCCTGTCTTT 
GAAATCCCCT CACCTCGCTG TTCCCGTGGC AATGAAGAAG AGGACAATGA GGAAGACGAG 
GAGAATGACT CAACAGCCAC TTTTACAGCT GAAAGTGATG GAGAGGACAG GGATGCTGAG 
GAGGAGGAGG AGGAGGAGGA GGTGGTAGTA TTGGGTCACA TAGCAGAGGG AGAGTTGTCT 
CCCTCCCTGA CTCCTAAATT TGCCCGATTG TTGTCAGAGG ACGAGTTTCT CCCAACTGCT 
AACGCAGTAA GTGCAGAAGT GATTGCGGGA GGGGGAGTAT TCTGCCCCAC AGCCATCTAC 
AGACCGTTTG TGGATAAAGC TCTGAAGCAG ATGGGGCTGC GTAAACTGAT CCTCAGACTG 
CACAAACTGA TGGACCGCTC TCTTCAGAGG TCCAGAGCAG CACTGCTCCG TCACACTCCT 
GCACTGGAGT TTGCAGATTT CCCTGACCCC ATGCTGTACA GTGATTACCT GCCAGAGCTA 
TCGCGCCATG AGTCCTGCAG TGGTCCGGCA CATCCGGAGC TTGGGGCAGA CCAGGTTTCC 
TGGGAGGATT TGCTGGACAT GGATCTCCCG TCCTTTCGGC CAGCGTTCCT TGTGCTGTGC 
AGAGTCCTTC TAAACGTCAT TCACGAATGC CTTAAACTGC GACTTGAACA GAGGCCTGCT 
GGGGAGCCTT CACTGCTCAG CATCAAACAG TTGGTGCGTG AGTGTAAGGA GGTTCTTAAA 
GGTGGTCTTC TGATGAAGCA GTATTATCAG TTCATGCTGC GAGGCGTGGT GACTGATGCT 
CAGGGCTTGC AGACGAATGC CAACATTGAT GAGTTTGAAG AGGATCTTCA CAAGATGCTG 
GTGGTCTACT TTGATTACAT GCACAGTTGG ATCCAGATGT TGCAGCAGCT GCCTCAGGCC 
TCTCACAGCT TAAAGAACCT GTTGGAGGAG GAGTGGAACT TCACCAAGGT CATCACTCCT 
TACATTCGAG GAGGGGAGGC CCAGTCCGGG AAGCTTTTTT GTGACATCGC TGGGATGCTG 
CTTAAATCCA CTGGAGAGTT CCTTGATGCC GGCCTGCAAA AGAGCGGTAA TGAATTCTGG 
GAAACTGCAG ATGACAGCAC CGCCTCAGAT GAGATCAGGC GGTCAGTTAT CGAGACTAGT 
CGGTCGCTCA AAGAGCTGTT CCACGAGGCC AGGGAACGAG CGTCCAAGGC TCTCGGCTTT 
GCCAAGATGC TTCGCAAGGA CTTGGAAGTT GCTGCAGATT TCAGCATCAC TAATGGGGTG 
ACTTGTCTCC TGGAGGCACT GAAGAAGAGA AACTATGTCA AGGTTCAGAT TCCAGGCTTG 
GAGGAGCTGC AGGTTTTTGT CCCTTGTGGC TTGATGGGTC AGCGGCCTCT CATCCTGCAG 
CTTCTCAATG CTGCTGCTGG CAAAGACTGC TCCAAGGAGC CTGATGAAAT TGCAGAGGAC 
GAGGCCTACC TGCTTATGAG TAAACACGGA GCTGGGGATT CCGCTACTGA CTCTGACTGG 
GCTCAGTGGG AAGGAGAGCT GCTCAAACTG GTTCCCCAGA TGGAAACTGT TGACACTTTA 
AGGGCCATGA AGGTAGAGAA TGTGCTCTTG ATTGTGATGC AGTCTGCTCA CCTGGTGGCC 
CAGCGAAAGG CCTTTCAGCA GTCCATGGAG GATGTGCTCA CTCTTAGCCG GGAGCAGACA 
TCTAGTCAGC CACTCATTGC GAGTGCGCTG GAGGAACTCA AGGATGAGGC ACTACAGCTA 
TGCATAAAGA TCAGCACTGC CATTGACCGA GTGGAGTTCA TGTTCACCAC AGAGTTTGAG 
GCAGAGGTGG AGGAGTCTGA GTCAGCCACT CTGCATCAGT ACTACAGGGA GGCAATGATA 
CAGGGCTACA ATTTTGCCTT CGAGTACCAC AAGGAGGTGG TGCGGCTAAT GTCAGGGGAG 
TTCAGACAAA GGATCGGGGA GCGCTACATT GCTTTTGCCC GCAAATGGAT GACCTATGTC 
CTGACCAAAT GTGAGAGTGG CAGGGGCACC AAGCCCAGGT GGGCGACTCA GGGGTTTGAC 
TTTCTTCAGG CTATCGAACC TGGCTTTATA TCAGCATTGC CAGAGGATGA CTTCTTGAAT 
TTGCAAGCTT TGATGAATGA ATGTATTGGA CATGTGATTG GAAAACCTCA TAGCCCAGTC 
ACCGGCCTGT ACATAGCTCC TAGAAACAGT CCTCGACCTG TAAAGGTGCC TCGCTGCCAT 
AGTGACCCAC CAAACCCCAA CCTGTTCATC CCTAATGCTG AAGGTTTCAG CTCTCGAAGT 
CTCCCCTGCG ACCTCCGGAA CCAGCTGTTC CCCAACGGCC CTCGCCCCGT CCCTCAGGGC 
CCGGGGGAAC AAAGCAACAC CAAAGCACCC AGCAGCAGCC CCAATGACGT CAGGGGATCC 
AGTTTCCATG AGAATGACCG CCTGTCGTCA GTTGCAGCAG AGTTGCATTT TAAATCTCTG 
AGCCGCCACT CCAGCCCCAC AGAGGACAGA GAAGAACCCT CTTATCCTAA GGGGGATCCT 
AACAGCACTG CACGCCGAAG CTGGGAGCTT CGCAACTTTA TCAGCCAGTC CAAGGACACA 
GCAGCAAGGC AAAGCCCCAT GGAGGCTGTC CGTCGCTCCA TTCGCAAGTT CGAGGACAAA 
CGCTATGCTG TGATGAAGCA GCGCAACATC ATCGGCCAAG TGTGTCACAC ACCCAAATCC 
TATGACAATG TCATGCATGT CGGGCTCAGG AAGGTGACCT TTAAGTGGCA GAGAGGCAAC 
AAGATAGGTG AGGGCCAGTA TGGGAAAGTG TACACCTGCA TCAATGTTGA CACTGGAGAA 
CTAATGGCCA TGAAGGAGAT CCGATTCCAG CCGAATGATC ACAAAACAAT CAAGGAGACT 
GCAGATGAGC TGAAAATATT TGAAGGCATT AAACACCCAA ACTTGGTGCG ATACTTTGGA 
GTGGAGCTCC ATCGGGAGGA GATGTACATC TTCATGGAGT ACTGTGACGA GGGCACCCTG 
GAGGAGGTGT CCAGACTGGG GCTGCAGGAA CATGTCATCA GGCTCTACAG TAAACAGATC 
ACTACAGCCA TCAATGTCCT CCATGAGCAT GGCATTGTCC ACCGCGATAT CAAGGGAGCC 
AACATCTTTT TGACATCGTC TGGCCTGATT AAGCTGGGTG ACTTTGGCTG TTCAGTGAAA 
TTGAGGAACA ACACACACAC CATGCCAGGG GAAGTGAACA GCACGCTGGG AACAGCAGCA 
TACATGGCAC CTGAAGTCAT CACCAGAGCA AAAGGGGAAG GTCACGGACG AGCGGCGGAC 
ATCTGGAGTT TGGGCTGTGT TCTCATTGAG ATGGTGACTG GAAAGAGGCC CTGGCACGAG 
TATGAGCACA ACTTCCAGAT CATGTACAAA GTGGGAATGG GCCATAAACC CCCCATCCCA 
GAGAAACTCA GCACAGAGGG GAAGGACTTC CTTGGTCACT GTCTGGAGAG TGAACCCAAA 
CGCCGCTGGA CAGCTAGCAT GCTGCTAGAC CACCCGTTTG TCAAGGTTTG TACGGATGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275675.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131220.1 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

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CloneID	OLa109670
Clone ID Related Accession (Same CDS sequence)	XM_019275675.1
Accession Version	XM_019275675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609438.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCTCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGAG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCGCTA CTGACTCTGA CTGGGCTCAG TGGGAAGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCTAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC AGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACTTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131220.1
CDS	302..4948
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGSFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWEDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSATDSDWAQWEGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275675.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275675.1

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCCCGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCTCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGAG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCGCTA CTGACTCTGA CTGGGCTCAG TGGGAAGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCTAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC AGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACTTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275672.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131217.2 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

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CloneID	OLa218133
Clone ID Related Accession (Same CDS sequence)	XM_019275672.2
Accession Version	XM_019275672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040013.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019275672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCGCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGTG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCACTA CTGACTCTGA CTGGGCTCAG TGGGATGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCCAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGCTCTCG AAGTCTCCCC TGCGACCTCC GGAACCAGCT GTTCCCCAAC 
GGCCCTCGCC CCGTCCCTCA GGGCCCGGGG GAACAAAGCA ACACCAAAGC ACCCAGCAGC 
AGCCCCAATG ACGTCAGGGG ATCCAGTTTC CATGAGAATG ACCGCCTGTC GTCGGTTGCA 
GCAGAGTTGC ATTTTAAATC TCTGAGCCGC CACTCCAGCC CCACAGAGGA CAGAGAAGAA 
CCCTCTTATC CTAAGGGGGA TCCTAACAGC ACTGCACGCC GAAGCTGGGA GCTTCGCAAC 
TTTATCAGCC AGTCCAAGGA CACAGCAGCA AGGCAAAGCC CCATGGAGGC TGTCCGTCGC 
TCCATTCGCA AGTTCGAGGA CAAACGCTAT GCTGTGATGA AGCAGCGCAA CATCATCGGC 
CAAGTGTGTC ACACACCCAA ATCCTATGAC AATGTCATGC ATGTCGGGCT CAGGAAGGTG 
ACCTTTAAGT GGCAGAGAGG CAACAAGATA GGTGAGGGCC AGTATGGGAA AGTGTACACC 
TGCATCAATG TTGACACTGG AGAACTAATG GCCATGAAGG AGATCCGATT CCAGCCGAAT 
GATCACAAAA CAATCAAGGA GACTGCAGAT GAGCTGAAAA TATTTGAAGG CATTAAACAC 
CCAAACCTGG TGCGATACTT TGGAGTGGAG CTCCATCGGG AGGAGATGTA CATCTTCATG 
GAGTACTGTG ACGAGGGCAC CCTGGAGGAG GTGTCCAGAC TGGGGCTGCA GGAACATGTC 
ATCAGGCTCT ACAGTAAACA GATCACTACA GCCATCAATG TCCTCCATGA GCATGGCATT 
GTCCACCGCG ATATCAAGGG AGCCAACATC TTTTTGACAT CGTCTGGCCT GATTAAGCTG 
GGTGACTTTG GCTGTTCAGT GAAATTGAGG AACAACACAC ACACCATGCC AGGGGAAGTG 
AACAGCACGC TGGGAACAGC AGCATACATG GCACCTGAAG TCATCACCAG AGCAAAAGGG 
GAAGGTCACG GACGAGCGGC GGACATCTGG AGTTTGGGCT GTGTTCTCAT TGAGATGGTG 
ACTGGAAAGA GGCCCTGGCA CGAGTATGAG CACAACTTCC AGATCATGTA CAAAGTGGGA 
ATGGGCCATA AACCCCCCAT CCCAGAGAAA CTCAGCACAG AGGGGAAGGA CTTCCTTGGT 
CACTGTCTGG AGAGTGAACC CAAACGCCGC TGGACAGCTA GCATGCTGCT AGACCACCCG 
TTTGTCAAGG TTTGTACGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131217.2
CDS	238..5007
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGAFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWVDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSTTDSDWAQWDGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275672.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275672.2

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCGCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGTG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCACTA CTGACTCTGA CTGGGCTCAG TGGGATGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCCAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGCTCTCG AAGTCTCCCC TGCGACCTCC GGAACCAGCT GTTCCCCAAC 
GGCCCTCGCC CCGTCCCTCA GGGCCCGGGG GAACAAAGCA ACACCAAAGC ACCCAGCAGC 
AGCCCCAATG ACGTCAGGGG ATCCAGTTTC CATGAGAATG ACCGCCTGTC GTCGGTTGCA 
GCAGAGTTGC ATTTTAAATC TCTGAGCCGC CACTCCAGCC CCACAGAGGA CAGAGAAGAA 
CCCTCTTATC CTAAGGGGGA TCCTAACAGC ACTGCACGCC GAAGCTGGGA GCTTCGCAAC 
TTTATCAGCC AGTCCAAGGA CACAGCAGCA AGGCAAAGCC CCATGGAGGC TGTCCGTCGC 
TCCATTCGCA AGTTCGAGGA CAAACGCTAT GCTGTGATGA AGCAGCGCAA CATCATCGGC 
CAAGTGTGTC ACACACCCAA ATCCTATGAC AATGTCATGC ATGTCGGGCT CAGGAAGGTG 
ACCTTTAAGT GGCAGAGAGG CAACAAGATA GGTGAGGGCC AGTATGGGAA AGTGTACACC 
TGCATCAATG TTGACACTGG AGAACTAATG GCCATGAAGG AGATCCGATT CCAGCCGAAT 
GATCACAAAA CAATCAAGGA GACTGCAGAT GAGCTGAAAA TATTTGAAGG CATTAAACAC 
CCAAACCTGG TGCGATACTT TGGAGTGGAG CTCCATCGGG AGGAGATGTA CATCTTCATG 
GAGTACTGTG ACGAGGGCAC CCTGGAGGAG GTGTCCAGAC TGGGGCTGCA GGAACATGTC 
ATCAGGCTCT ACAGTAAACA GATCACTACA GCCATCAATG TCCTCCATGA GCATGGCATT 
GTCCACCGCG ATATCAAGGG AGCCAACATC TTTTTGACAT CGTCTGGCCT GATTAAGCTG 
GGTGACTTTG GCTGTTCAGT GAAATTGAGG AACAACACAC ACACCATGCC AGGGGAAGTG 
AACAGCACGC TGGGAACAGC AGCATACATG GCACCTGAAG TCATCACCAG AGCAAAAGGG 
GAAGGTCACG GACGAGCGGC GGACATCTGG AGTTTGGGCT GTGTTCTCAT TGAGATGGTG 
ACTGGAAAGA GGCCCTGGCA CGAGTATGAG CACAACTTCC AGATCATGTA CAAAGTGGGA 
ATGGGCCATA AACCCCCCAT CCCAGAGAAA CTCAGCACAG AGGGGAAGGA CTTCCTTGGT 
CACTGTCTGG AGAGTGAACC CAAACGCCGC TGGACAGCTA GCATGCTGCT AGACCACCCG 
TTTGTCAAGG TTTGTACGGA TGAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275673.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131218.2 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

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CloneID	OLa218134
Clone ID Related Accession (Same CDS sequence)	XM_019275673.2
Accession Version	XM_019275673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040013.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019275673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG ACCTTAGCCC ATCTGGCCTG 
AGAGAAAGTC CCAGGCCAGT GGAGACCCCT GAAGAGCACA GCTACAAGCA GGGCAAGAAG 
CAGAGGGCCA CACTGCGCTC CACGGAGAGA GACCACAAGA AGACCTTTGA AGGCGCCTTC 
ATGCTGGACC CACTCTCAAA GTCGACTCAT TTCGGTTCCC TCAATATGGA TCCCAGGAAG 
CATTACTTGA GCTTGGGTTG CAGCAGTGGC AAACTTCCTG TGTCTATGCC CCATATGGCT 
CGGACCCACC GCCAGACCTC CAGGACAGAC TGTCCTGCTG ACCGCCTCAA GTTCTTTGAA 
ACTCTGCGGC TGCTCCTCAA GCTCACGTCT ATGTCCTCCA AGAGGAAGGA GAAGGAGCAG 
AGAGGCCTGG AGAACATGGC CTTCATGGGT CACAACAACG AGGTCATTTG GTTGGAGCTG 
CAGGCTTGGC ATGCACGGCG CTCCACCGGC GACCAAGACC TTTTTCTTTT TACTGCCCGT 
CAAGCCATCC CTGACATCAT CAATGAGGTG CTGCACTTTA AGGTCAACTA TGCCAGCCTG 
AGAGGGGCCC AGTGTTCAGT CGAGGGGGAA TATCAGAGTG TCCCAGATCT AGTCTGTGGA 
GAGGAGAGAG AGCCTGCCGT AGCAGAGACC AACCACTGTG GAGTAGATCC CTGGGGCTTT 
AGTGCCTTCC CCTCATCAGC AATGAATGCA GCAGAGCCTC TGGGCTCTGG CGCTGTTTGC 
AGGGAGCATC TTCAGCGCCA GCGGCTGGCA TTTGAGCAGG TCAAACATGT TATAGAGTTG 
CTGGAGTCAG TGGAGGCTTT GTACCCCTCT TTGCAGGCCC TGCAGAAGGA CTATGAAAAG 
TATGCAGCAC GAGATTTCCA GGGCAGGGTG CAGGCGCTCT GTCTGTGGCT CAATATCACC 
CAGGACCTCA ACCAGAAGCT GCGTGTTATG GCAACTGTGC TGGGCCTCCA TGATCTATCT 
CGTATCGGGT GGCCTGTCTT TGAAATCCCC TCACCTCGCT GTTCCCGTGG CAATGAAGAA 
GAGGACAATG AGGAAGACGA GGAGAATGAC TCAACAGCCA CTTTTACAGC TGAAAGTGAT 
GGAGAGGACA GGGATGCTGA GGAGGAGGAG GAGGAGGAGG AGGTGGTAGT ATTGGGTCAC 
ATAGCAGAGG GAGAGTTGTC TCCCTCCCTG ACTCCTAAAT TTGCCCGATT GTTGTCAGAG 
GACGAGTTTC TCCCAACTGC TAACGCAGTA AGTGCAGAAG TGATTGCGGG AGGGGGAGTA 
TTCTGCCCCA CAGCCATCTA CAGACCGTTT GTGGATAAAG CTCTGAAGCA GATGGGGCTG 
CGTAAACTGA TCCTCAGACT GCACAAACTG ATGGACCGCT CTCTTCAGAG GTCCAGAGCA 
GCACTGCTCC GTCACACTCC TGCACTGGAG TTTGCAGATT TCCCTGACCC CATGCTGTAC 
AGTGATTACC TGCCAGAGCT ATCGCGCCAT GAGTCCTGCA GTGGTCCGGC ACATCCGGAG 
CTTGGGGCAG ACCAGGTTTC CTGGGTGGAT TTGCTGGACA TGGATCTCCC GTCCTTTCGG 
CCAGCGTTCC TTGTGCTGTG CAGAGTCCTT CTAAACGTCA TTCACGAATG CCTTAAACTG 
CGACTTGAAC AGAGGCCTGC TGGGGAGCCT TCACTGCTCA GCATCAAACA GTTGGTGCGT 
GAGTGTAAGG AGGTTCTTAA AGGTGGTCTT CTGATGAAGC AGTATTATCA GTTCATGCTG 
CGAGGCGTGG TGACTGATGC TCAGGGCTTG CAGACGAATG CCAACATTGA TGAGTTTGAA 
GAGGATCTTC ACAAGATGCT GGTGGTCTAC TTTGATTACA TGCACAGTTG GATCCAGATG 
TTGCAGCAGC TGCCTCAGGC CTCTCACAGC TTAAAGAACC TGTTGGAGGA GGAGTGGAAC 
TTCACCAAGG TCATCACTCC TTACATTCGA GGAGGGGAGG CCCAGTCCGG GAAGCTTTTT 
TGTGACATCG CTGGGATGCT GCTTAAATCC ACTGGAGAGT TCCTTGATGC CGGCCTGCAA 
AAGAGCGGTA ATGAATTCTG GGAAACTGCA GATGACAGCA CCGCCTCAGA TGAGATCAGG 
CGGTCAGTTA TCGAGACTAG TCGGTCGCTC AAAGAGCTGT TCCACGAGGC CAGGGAACGA 
GCGTCCAAGG CTCTCGGCTT TGCCAAGATG CTTCGCAAGG ACTTGGAAGT TGCTGCAGAT 
TTCAGCATCA CTAATGGGGT GACTTGTCTC CTGGAGGCAC TGAAGAAGAG AAACTATGTC 
AAGGTTCAGA TTCCAGGCTT GGAGGAGCTG CAGGTTTTTG TCCCTTGTGG CTTGATGGGT 
CAGCGGCCTC TCATCCTGCA GCTTCTCAAT GCTGCTGCTG GCAAAGACTG CTCCAAGGAG 
CCTGATGAAA TTGCAGAGGA CGAGGCCTAC CTGCTTATGA GTAAACACGG AGCTGGGGAT 
TCCACTACTG ACTCTGACTG GGCTCAGTGG GATGGAGAGC TGCTCAAACT GGTTCCCCAG 
ATGGAAACTG TTGACACTTT AAGGGCCATG AAGGTAGAGA ATGTGCTCTT GATTGTGATG 
CAGTCTGCTC ACCTGGTGGC CCAGCGAAAG GCCTTTCAGC AGTCCATGGA GGATGTGCTC 
ACTCTTAGCC GGGAGCAGAC ATCTAGTCAG CCACTCATTG CGAGTGCGCT GGAGGAACTC 
AAGGATGAGG CACTACAGCT ATGCATAAAG ATCAGCACTG CCATTGACCG AGTGGAGTTC 
ATGTTCACCA CAGAGTTTGA GGCAGAGGTG GAGGAGTCTG AGTCAGCCAC TCTGCATCAG 
TACTACAGGG AGGCAATGAT ACAGGGCTAC AATTTTGCCT TCGAGTACCA CAAGGAGGTG 
GTGCGGCTAA TGTCAGGGGA GTTCAGACAA AGGATCGGGG AGCGCTACAT TGCTTTTGCC 
CGCAAATGGA TGACCTATGT CCTGACCAAA TGTGAGAGTG GCAGGGGCAC CAAGCCCAGG 
TGGGCGACTC AGGGGTTTGA CTTTCTTCAG GCTATCGAAC CTGGCTTTAT ATCAGCATTG 
CCAGAGGATG ACTTCTTGAA TTTGCAAGCT TTGATGAATG AATGTATTGG ACATGTGATT 
GGAAAACCTC ATAGCCCAGT CACCGGCCTG TACATAGCTC CCAGAAACAG TCCTCGACCT 
GTAAAGGTGC CTCGCTGCCA TAGTGACCCA CCAAACCCCA ACCTGTTCAT CCCTAATGCT 
GAAGGTTTCA GCTCTCGAAG TCTCCCCTGC GACCTCCGGA ACCAGCTGTT CCCCAACGGC 
CCTCGCCCCG TCCCTCAGGG CCCGGGGGAA CAAAGCAACA CCAAAGCACC CAGCAGCAGC 
CCCAATGACG TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC GGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACCTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131218.2
CDS	240..5006
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKDLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGAFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWVDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSTTDSDWAQWDGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275673.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275673.2

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG ACCTTAGCCC ATCTGGCCTG 
AGAGAAAGTC CCAGGCCAGT GGAGACCCCT GAAGAGCACA GCTACAAGCA GGGCAAGAAG 
CAGAGGGCCA CACTGCGCTC CACGGAGAGA GACCACAAGA AGACCTTTGA AGGCGCCTTC 
ATGCTGGACC CACTCTCAAA GTCGACTCAT TTCGGTTCCC TCAATATGGA TCCCAGGAAG 
CATTACTTGA GCTTGGGTTG CAGCAGTGGC AAACTTCCTG TGTCTATGCC CCATATGGCT 
CGGACCCACC GCCAGACCTC CAGGACAGAC TGTCCTGCTG ACCGCCTCAA GTTCTTTGAA 
ACTCTGCGGC TGCTCCTCAA GCTCACGTCT ATGTCCTCCA AGAGGAAGGA GAAGGAGCAG 
AGAGGCCTGG AGAACATGGC CTTCATGGGT CACAACAACG AGGTCATTTG GTTGGAGCTG 
CAGGCTTGGC ATGCACGGCG CTCCACCGGC GACCAAGACC TTTTTCTTTT TACTGCCCGT 
CAAGCCATCC CTGACATCAT CAATGAGGTG CTGCACTTTA AGGTCAACTA TGCCAGCCTG 
AGAGGGGCCC AGTGTTCAGT CGAGGGGGAA TATCAGAGTG TCCCAGATCT AGTCTGTGGA 
GAGGAGAGAG AGCCTGCCGT AGCAGAGACC AACCACTGTG GAGTAGATCC CTGGGGCTTT 
AGTGCCTTCC CCTCATCAGC AATGAATGCA GCAGAGCCTC TGGGCTCTGG CGCTGTTTGC 
AGGGAGCATC TTCAGCGCCA GCGGCTGGCA TTTGAGCAGG TCAAACATGT TATAGAGTTG 
CTGGAGTCAG TGGAGGCTTT GTACCCCTCT TTGCAGGCCC TGCAGAAGGA CTATGAAAAG 
TATGCAGCAC GAGATTTCCA GGGCAGGGTG CAGGCGCTCT GTCTGTGGCT CAATATCACC 
CAGGACCTCA ACCAGAAGCT GCGTGTTATG GCAACTGTGC TGGGCCTCCA TGATCTATCT 
CGTATCGGGT GGCCTGTCTT TGAAATCCCC TCACCTCGCT GTTCCCGTGG CAATGAAGAA 
GAGGACAATG AGGAAGACGA GGAGAATGAC TCAACAGCCA CTTTTACAGC TGAAAGTGAT 
GGAGAGGACA GGGATGCTGA GGAGGAGGAG GAGGAGGAGG AGGTGGTAGT ATTGGGTCAC 
ATAGCAGAGG GAGAGTTGTC TCCCTCCCTG ACTCCTAAAT TTGCCCGATT GTTGTCAGAG 
GACGAGTTTC TCCCAACTGC TAACGCAGTA AGTGCAGAAG TGATTGCGGG AGGGGGAGTA 
TTCTGCCCCA CAGCCATCTA CAGACCGTTT GTGGATAAAG CTCTGAAGCA GATGGGGCTG 
CGTAAACTGA TCCTCAGACT GCACAAACTG ATGGACCGCT CTCTTCAGAG GTCCAGAGCA 
GCACTGCTCC GTCACACTCC TGCACTGGAG TTTGCAGATT TCCCTGACCC CATGCTGTAC 
AGTGATTACC TGCCAGAGCT ATCGCGCCAT GAGTCCTGCA GTGGTCCGGC ACATCCGGAG 
CTTGGGGCAG ACCAGGTTTC CTGGGTGGAT TTGCTGGACA TGGATCTCCC GTCCTTTCGG 
CCAGCGTTCC TTGTGCTGTG CAGAGTCCTT CTAAACGTCA TTCACGAATG CCTTAAACTG 
CGACTTGAAC AGAGGCCTGC TGGGGAGCCT TCACTGCTCA GCATCAAACA GTTGGTGCGT 
GAGTGTAAGG AGGTTCTTAA AGGTGGTCTT CTGATGAAGC AGTATTATCA GTTCATGCTG 
CGAGGCGTGG TGACTGATGC TCAGGGCTTG CAGACGAATG CCAACATTGA TGAGTTTGAA 
GAGGATCTTC ACAAGATGCT GGTGGTCTAC TTTGATTACA TGCACAGTTG GATCCAGATG 
TTGCAGCAGC TGCCTCAGGC CTCTCACAGC TTAAAGAACC TGTTGGAGGA GGAGTGGAAC 
TTCACCAAGG TCATCACTCC TTACATTCGA GGAGGGGAGG CCCAGTCCGG GAAGCTTTTT 
TGTGACATCG CTGGGATGCT GCTTAAATCC ACTGGAGAGT TCCTTGATGC CGGCCTGCAA 
AAGAGCGGTA ATGAATTCTG GGAAACTGCA GATGACAGCA CCGCCTCAGA TGAGATCAGG 
CGGTCAGTTA TCGAGACTAG TCGGTCGCTC AAAGAGCTGT TCCACGAGGC CAGGGAACGA 
GCGTCCAAGG CTCTCGGCTT TGCCAAGATG CTTCGCAAGG ACTTGGAAGT TGCTGCAGAT 
TTCAGCATCA CTAATGGGGT GACTTGTCTC CTGGAGGCAC TGAAGAAGAG AAACTATGTC 
AAGGTTCAGA TTCCAGGCTT GGAGGAGCTG CAGGTTTTTG TCCCTTGTGG CTTGATGGGT 
CAGCGGCCTC TCATCCTGCA GCTTCTCAAT GCTGCTGCTG GCAAAGACTG CTCCAAGGAG 
CCTGATGAAA TTGCAGAGGA CGAGGCCTAC CTGCTTATGA GTAAACACGG AGCTGGGGAT 
TCCACTACTG ACTCTGACTG GGCTCAGTGG GATGGAGAGC TGCTCAAACT GGTTCCCCAG 
ATGGAAACTG TTGACACTTT AAGGGCCATG AAGGTAGAGA ATGTGCTCTT GATTGTGATG 
CAGTCTGCTC ACCTGGTGGC CCAGCGAAAG GCCTTTCAGC AGTCCATGGA GGATGTGCTC 
ACTCTTAGCC GGGAGCAGAC ATCTAGTCAG CCACTCATTG CGAGTGCGCT GGAGGAACTC 
AAGGATGAGG CACTACAGCT ATGCATAAAG ATCAGCACTG CCATTGACCG AGTGGAGTTC 
ATGTTCACCA CAGAGTTTGA GGCAGAGGTG GAGGAGTCTG AGTCAGCCAC TCTGCATCAG 
TACTACAGGG AGGCAATGAT ACAGGGCTAC AATTTTGCCT TCGAGTACCA CAAGGAGGTG 
GTGCGGCTAA TGTCAGGGGA GTTCAGACAA AGGATCGGGG AGCGCTACAT TGCTTTTGCC 
CGCAAATGGA TGACCTATGT CCTGACCAAA TGTGAGAGTG GCAGGGGCAC CAAGCCCAGG 
TGGGCGACTC AGGGGTTTGA CTTTCTTCAG GCTATCGAAC CTGGCTTTAT ATCAGCATTG 
CCAGAGGATG ACTTCTTGAA TTTGCAAGCT TTGATGAATG AATGTATTGG ACATGTGATT 
GGAAAACCTC ATAGCCCAGT CACCGGCCTG TACATAGCTC CCAGAAACAG TCCTCGACCT 
GTAAAGGTGC CTCGCTGCCA TAGTGACCCA CCAAACCCCA ACCTGTTCAT CCCTAATGCT 
GAAGGTTTCA GCTCTCGAAG TCTCCCCTGC GACCTCCGGA ACCAGCTGTT CCCCAACGGC 
CCTCGCCCCG TCCCTCAGGG CCCGGGGGAA CAAAGCAACA CCAAAGCACC CAGCAGCAGC 
CCCAATGACG TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC GGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACCTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275674.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131219.2 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLa218135
Clone ID Related Accession (Same CDS sequence)	XM_019275674.2
Accession Version	XM_019275674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040013.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019275674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGGC AGGCCAAGCC AGTGCAAGAT GCATCCCAAC AGAACTCTGC GGTGGGTGAA 
TCCTGGGACT CTCCCAGCGA GGAAGAGGAA GCTCTGTATG GCACTTCACC TCCTTATAAC 
CCACGTCAGA TGAAACGCAT GTCTGGCAAA CATCAAAGGA GCCAAGCCAG GAGTGCTGGT 
CGGTCTCCTA ACAAGGCAGA CCTTAGCCCA TCTGGCCTGA GAGAAAGTCC CAGGCCAGTG 
GAGACCCCTG AAGAGCACAG CTACAAGCAG GGCAAGAAGC AGAGGGCCAC ACTGCGCTCC 
ACGGAGAGAG ACCACAAGAA GACCTTTGAA GGCGCCTTCA TGCTGGACCC ACTCTCAAAG 
TCGACTCATT TCGGTTCCCT CAATATGGAT CCCAGGAAGC ATTACTTGAG CTTGGGTTGC 
AGCAGTGGCA AACTTCCTGT GTCTATGCCC CATATGGCTC GGACCCACCG CCAGACCTCC 
AGGACAGACT GTCCTGCTGA CCGCCTCAAG TTCTTTGAAA CTCTGCGGCT GCTCCTCAAG 
CTCACGTCTA TGTCCTCCAA GAGGAAGGAG AAGGAGCAGA GAGGCCTGGA GAACATGGCC 
TTCATGGGTC ACAACAACGA GGTCATTTGG TTGGAGCTGC AGGCTTGGCA TGCACGGCGC 
TCCACCGGCG ACCAAGACCT TTTTCTTTTT ACTGCCCGTC AAGCCATCCC TGACATCATC 
AATGAGGTGC TGCACTTTAA GGTCAACTAT GCCAGCCTGA GAGGGGCCCA GTGTTCAGTC 
GAGGGGGAAT ATCAGAGTGT CCCAGATCTA GTCTGTGGAG AGGAGAGAGA GCCTGCCGTA 
GCAGAGACCA ACCACTGTGG AGTAGATCCC TGGGGCTTTA GTGCCTTCCC CTCATCAGCA 
ATGAATGCAG CAGAGCCTCT GGGCTCTGGC GCTGTTTGCA GGGAGCATCT TCAGCGCCAG 
CGGCTGGCAT TTGAGCAGGT CAAACATGTT ATAGAGTTGC TGGAGTCAGT GGAGGCTTTG 
TACCCCTCTT TGCAGGCCCT GCAGAAGGAC TATGAAAAGT ATGCAGCACG AGATTTCCAG 
GGCAGGGTGC AGGCGCTCTG TCTGTGGCTC AATATCACCC AGGACCTCAA CCAGAAGCTG 
CGTGTTATGG CAACTGTGCT GGGCCTCCAT GATCTATCTC GTATCGGGTG GCCTGTCTTT 
GAAATCCCCT CACCTCGCTG TTCCCGTGGC AATGAAGAAG AGGACAATGA GGAAGACGAG 
GAGAATGACT CAACAGCCAC TTTTACAGCT GAAAGTGATG GAGAGGACAG GGATGCTGAG 
GAGGAGGAGG AGGAGGAGGA GGTGGTAGTA TTGGGTCACA TAGCAGAGGG AGAGTTGTCT 
CCCTCCCTGA CTCCTAAATT TGCCCGATTG TTGTCAGAGG ACGAGTTTCT CCCAACTGCT 
AACGCAGTAA GTGCAGAAGT GATTGCGGGA GGGGGAGTAT TCTGCCCCAC AGCCATCTAC 
AGACCGTTTG TGGATAAAGC TCTGAAGCAG ATGGGGCTGC GTAAACTGAT CCTCAGACTG 
CACAAACTGA TGGACCGCTC TCTTCAGAGG TCCAGAGCAG CACTGCTCCG TCACACTCCT 
GCACTGGAGT TTGCAGATTT CCCTGACCCC ATGCTGTACA GTGATTACCT GCCAGAGCTA 
TCGCGCCATG AGTCCTGCAG TGGTCCGGCA CATCCGGAGC TTGGGGCAGA CCAGGTTTCC 
TGGGTGGATT TGCTGGACAT GGATCTCCCG TCCTTTCGGC CAGCGTTCCT TGTGCTGTGC 
AGAGTCCTTC TAAACGTCAT TCACGAATGC CTTAAACTGC GACTTGAACA GAGGCCTGCT 
GGGGAGCCTT CACTGCTCAG CATCAAACAG TTGGTGCGTG AGTGTAAGGA GGTTCTTAAA 
GGTGGTCTTC TGATGAAGCA GTATTATCAG TTCATGCTGC GAGGCGTGGT GACTGATGCT 
CAGGGCTTGC AGACGAATGC CAACATTGAT GAGTTTGAAG AGGATCTTCA CAAGATGCTG 
GTGGTCTACT TTGATTACAT GCACAGTTGG ATCCAGATGT TGCAGCAGCT GCCTCAGGCC 
TCTCACAGCT TAAAGAACCT GTTGGAGGAG GAGTGGAACT TCACCAAGGT CATCACTCCT 
TACATTCGAG GAGGGGAGGC CCAGTCCGGG AAGCTTTTTT GTGACATCGC TGGGATGCTG 
CTTAAATCCA CTGGAGAGTT CCTTGATGCC GGCCTGCAAA AGAGCGGTAA TGAATTCTGG 
GAAACTGCAG ATGACAGCAC CGCCTCAGAT GAGATCAGGC GGTCAGTTAT CGAGACTAGT 
CGGTCGCTCA AAGAGCTGTT CCACGAGGCC AGGGAACGAG CGTCCAAGGC TCTCGGCTTT 
GCCAAGATGC TTCGCAAGGA CTTGGAAGTT GCTGCAGATT TCAGCATCAC TAATGGGGTG 
ACTTGTCTCC TGGAGGCACT GAAGAAGAGA AACTATGTCA AGGTTCAGAT TCCAGGCTTG 
GAGGAGCTGC AGGTTTTTGT CCCTTGTGGC TTGATGGGTC AGCGGCCTCT CATCCTGCAG 
CTTCTCAATG CTGCTGCTGG CAAAGACTGC TCCAAGGAGC CTGATGAAAT TGCAGAGGAC 
GAGGCCTACC TGCTTATGAG TAAACACGGA GCTGGGGATT CCACTACTGA CTCTGACTGG 
GCTCAGTGGG ATGGAGAGCT GCTCAAACTG GTTCCCCAGA TGGAAACTGT TGACACTTTA 
AGGGCCATGA AGGTAGAGAA TGTGCTCTTG ATTGTGATGC AGTCTGCTCA CCTGGTGGCC 
CAGCGAAAGG CCTTTCAGCA GTCCATGGAG GATGTGCTCA CTCTTAGCCG GGAGCAGACA 
TCTAGTCAGC CACTCATTGC GAGTGCGCTG GAGGAACTCA AGGATGAGGC ACTACAGCTA 
TGCATAAAGA TCAGCACTGC CATTGACCGA GTGGAGTTCA TGTTCACCAC AGAGTTTGAG 
GCAGAGGTGG AGGAGTCTGA GTCAGCCACT CTGCATCAGT ACTACAGGGA GGCAATGATA 
CAGGGCTACA ATTTTGCCTT CGAGTACCAC AAGGAGGTGG TGCGGCTAAT GTCAGGGGAG 
TTCAGACAAA GGATCGGGGA GCGCTACATT GCTTTTGCCC GCAAATGGAT GACCTATGTC 
CTGACCAAAT GTGAGAGTGG CAGGGGCACC AAGCCCAGGT GGGCGACTCA GGGGTTTGAC 
TTTCTTCAGG CTATCGAACC TGGCTTTATA TCAGCATTGC CAGAGGATGA CTTCTTGAAT 
TTGCAAGCTT TGATGAATGA ATGTATTGGA CATGTGATTG GAAAACCTCA TAGCCCAGTC 
ACCGGCCTGT ACATAGCTCC CAGAAACAGT CCTCGACCTG TAAAGGTGCC TCGCTGCCAT 
AGTGACCCAC CAAACCCCAA CCTGTTCATC CCTAATGCTG AAGGTTTCAG CTCTCGAAGT 
CTCCCCTGCG ACCTCCGGAA CCAGCTGTTC CCCAACGGCC CTCGCCCCGT CCCTCAGGGC 
CCGGGGGAAC AAAGCAACAC CAAAGCACCC AGCAGCAGCC CCAATGACGT CAGGGGATCC 
AGTTTCCATG AGAATGACCG CCTGTCGTCG GTTGCAGCAG AGTTGCATTT TAAATCTCTG 
AGCCGCCACT CCAGCCCCAC AGAGGACAGA GAAGAACCCT CTTATCCTAA GGGGGATCCT 
AACAGCACTG CACGCCGAAG CTGGGAGCTT CGCAACTTTA TCAGCCAGTC CAAGGACACA 
GCAGCAAGGC AAAGCCCCAT GGAGGCTGTC CGTCGCTCCA TTCGCAAGTT CGAGGACAAA 
CGCTATGCTG TGATGAAGCA GCGCAACATC ATCGGCCAAG TGTGTCACAC ACCCAAATCC 
TATGACAATG TCATGCATGT CGGGCTCAGG AAGGTGACCT TTAAGTGGCA GAGAGGCAAC 
AAGATAGGTG AGGGCCAGTA TGGGAAAGTG TACACCTGCA TCAATGTTGA CACTGGAGAA 
CTAATGGCCA TGAAGGAGAT CCGATTCCAG CCGAATGATC ACAAAACAAT CAAGGAGACT 
GCAGATGAGC TGAAAATATT TGAAGGCATT AAACACCCAA ACCTGGTGCG ATACTTTGGA 
GTGGAGCTCC ATCGGGAGGA GATGTACATC TTCATGGAGT ACTGTGACGA GGGCACCCTG 
GAGGAGGTGT CCAGACTGGG GCTGCAGGAA CATGTCATCA GGCTCTACAG TAAACAGATC 
ACTACAGCCA TCAATGTCCT CCATGAGCAT GGCATTGTCC ACCGCGATAT CAAGGGAGCC 
AACATCTTTT TGACATCGTC TGGCCTGATT AAGCTGGGTG ACTTTGGCTG TTCAGTGAAA 
TTGAGGAACA ACACACACAC CATGCCAGGG GAAGTGAACA GCACGCTGGG AACAGCAGCA 
TACATGGCAC CTGAAGTCAT CACCAGAGCA AAAGGGGAAG GTCACGGACG AGCGGCGGAC 
ATCTGGAGTT TGGGCTGTGT TCTCATTGAG ATGGTGACTG GAAAGAGGCC CTGGCACGAG 
TATGAGCACA ACTTCCAGAT CATGTACAAA GTGGGAATGG GCCATAAACC CCCCATCCCA 
GAGAAACTCA GCACAGAGGG GAAGGACTTC CTTGGTCACT GTCTGGAGAG TGAACCCAAA 
CGCCGCTGGA CAGCTAGCAT GCTGCTAGAC CACCCGTTTG TCAAGGTTTG TACGGATGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131219.2
CDS	214..4959
Translation	MIRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGAFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWVDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSTTDSDWAQWDGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFSSRSLPCDLRNQLFPNGPRPVPQGPGEQSNTKAPSSSPNDVRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275674.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275674.2

ORF Insert Sequence:

ATGATCAGGC AGGCCAAGCC AGTGCAAGAT GCATCCCAAC AGAACTCTGC GGTGGGTGAA 
TCCTGGGACT CTCCCAGCGA GGAAGAGGAA GCTCTGTATG GCACTTCACC TCCTTATAAC 
CCACGTCAGA TGAAACGCAT GTCTGGCAAA CATCAAAGGA GCCAAGCCAG GAGTGCTGGT 
CGGTCTCCTA ACAAGGCAGA CCTTAGCCCA TCTGGCCTGA GAGAAAGTCC CAGGCCAGTG 
GAGACCCCTG AAGAGCACAG CTACAAGCAG GGCAAGAAGC AGAGGGCCAC ACTGCGCTCC 
ACGGAGAGAG ACCACAAGAA GACCTTTGAA GGCGCCTTCA TGCTGGACCC ACTCTCAAAG 
TCGACTCATT TCGGTTCCCT CAATATGGAT CCCAGGAAGC ATTACTTGAG CTTGGGTTGC 
AGCAGTGGCA AACTTCCTGT GTCTATGCCC CATATGGCTC GGACCCACCG CCAGACCTCC 
AGGACAGACT GTCCTGCTGA CCGCCTCAAG TTCTTTGAAA CTCTGCGGCT GCTCCTCAAG 
CTCACGTCTA TGTCCTCCAA GAGGAAGGAG AAGGAGCAGA GAGGCCTGGA GAACATGGCC 
TTCATGGGTC ACAACAACGA GGTCATTTGG TTGGAGCTGC AGGCTTGGCA TGCACGGCGC 
TCCACCGGCG ACCAAGACCT TTTTCTTTTT ACTGCCCGTC AAGCCATCCC TGACATCATC 
AATGAGGTGC TGCACTTTAA GGTCAACTAT GCCAGCCTGA GAGGGGCCCA GTGTTCAGTC 
GAGGGGGAAT ATCAGAGTGT CCCAGATCTA GTCTGTGGAG AGGAGAGAGA GCCTGCCGTA 
GCAGAGACCA ACCACTGTGG AGTAGATCCC TGGGGCTTTA GTGCCTTCCC CTCATCAGCA 
ATGAATGCAG CAGAGCCTCT GGGCTCTGGC GCTGTTTGCA GGGAGCATCT TCAGCGCCAG 
CGGCTGGCAT TTGAGCAGGT CAAACATGTT ATAGAGTTGC TGGAGTCAGT GGAGGCTTTG 
TACCCCTCTT TGCAGGCCCT GCAGAAGGAC TATGAAAAGT ATGCAGCACG AGATTTCCAG 
GGCAGGGTGC AGGCGCTCTG TCTGTGGCTC AATATCACCC AGGACCTCAA CCAGAAGCTG 
CGTGTTATGG CAACTGTGCT GGGCCTCCAT GATCTATCTC GTATCGGGTG GCCTGTCTTT 
GAAATCCCCT CACCTCGCTG TTCCCGTGGC AATGAAGAAG AGGACAATGA GGAAGACGAG 
GAGAATGACT CAACAGCCAC TTTTACAGCT GAAAGTGATG GAGAGGACAG GGATGCTGAG 
GAGGAGGAGG AGGAGGAGGA GGTGGTAGTA TTGGGTCACA TAGCAGAGGG AGAGTTGTCT 
CCCTCCCTGA CTCCTAAATT TGCCCGATTG TTGTCAGAGG ACGAGTTTCT CCCAACTGCT 
AACGCAGTAA GTGCAGAAGT GATTGCGGGA GGGGGAGTAT TCTGCCCCAC AGCCATCTAC 
AGACCGTTTG TGGATAAAGC TCTGAAGCAG ATGGGGCTGC GTAAACTGAT CCTCAGACTG 
CACAAACTGA TGGACCGCTC TCTTCAGAGG TCCAGAGCAG CACTGCTCCG TCACACTCCT 
GCACTGGAGT TTGCAGATTT CCCTGACCCC ATGCTGTACA GTGATTACCT GCCAGAGCTA 
TCGCGCCATG AGTCCTGCAG TGGTCCGGCA CATCCGGAGC TTGGGGCAGA CCAGGTTTCC 
TGGGTGGATT TGCTGGACAT GGATCTCCCG TCCTTTCGGC CAGCGTTCCT TGTGCTGTGC 
AGAGTCCTTC TAAACGTCAT TCACGAATGC CTTAAACTGC GACTTGAACA GAGGCCTGCT 
GGGGAGCCTT CACTGCTCAG CATCAAACAG TTGGTGCGTG AGTGTAAGGA GGTTCTTAAA 
GGTGGTCTTC TGATGAAGCA GTATTATCAG TTCATGCTGC GAGGCGTGGT GACTGATGCT 
CAGGGCTTGC AGACGAATGC CAACATTGAT GAGTTTGAAG AGGATCTTCA CAAGATGCTG 
GTGGTCTACT TTGATTACAT GCACAGTTGG ATCCAGATGT TGCAGCAGCT GCCTCAGGCC 
TCTCACAGCT TAAAGAACCT GTTGGAGGAG GAGTGGAACT TCACCAAGGT CATCACTCCT 
TACATTCGAG GAGGGGAGGC CCAGTCCGGG AAGCTTTTTT GTGACATCGC TGGGATGCTG 
CTTAAATCCA CTGGAGAGTT CCTTGATGCC GGCCTGCAAA AGAGCGGTAA TGAATTCTGG 
GAAACTGCAG ATGACAGCAC CGCCTCAGAT GAGATCAGGC GGTCAGTTAT CGAGACTAGT 
CGGTCGCTCA AAGAGCTGTT CCACGAGGCC AGGGAACGAG CGTCCAAGGC TCTCGGCTTT 
GCCAAGATGC TTCGCAAGGA CTTGGAAGTT GCTGCAGATT TCAGCATCAC TAATGGGGTG 
ACTTGTCTCC TGGAGGCACT GAAGAAGAGA AACTATGTCA AGGTTCAGAT TCCAGGCTTG 
GAGGAGCTGC AGGTTTTTGT CCCTTGTGGC TTGATGGGTC AGCGGCCTCT CATCCTGCAG 
CTTCTCAATG CTGCTGCTGG CAAAGACTGC TCCAAGGAGC CTGATGAAAT TGCAGAGGAC 
GAGGCCTACC TGCTTATGAG TAAACACGGA GCTGGGGATT CCACTACTGA CTCTGACTGG 
GCTCAGTGGG ATGGAGAGCT GCTCAAACTG GTTCCCCAGA TGGAAACTGT TGACACTTTA 
AGGGCCATGA AGGTAGAGAA TGTGCTCTTG ATTGTGATGC AGTCTGCTCA CCTGGTGGCC 
CAGCGAAAGG CCTTTCAGCA GTCCATGGAG GATGTGCTCA CTCTTAGCCG GGAGCAGACA 
TCTAGTCAGC CACTCATTGC GAGTGCGCTG GAGGAACTCA AGGATGAGGC ACTACAGCTA 
TGCATAAAGA TCAGCACTGC CATTGACCGA GTGGAGTTCA TGTTCACCAC AGAGTTTGAG 
GCAGAGGTGG AGGAGTCTGA GTCAGCCACT CTGCATCAGT ACTACAGGGA GGCAATGATA 
CAGGGCTACA ATTTTGCCTT CGAGTACCAC AAGGAGGTGG TGCGGCTAAT GTCAGGGGAG 
TTCAGACAAA GGATCGGGGA GCGCTACATT GCTTTTGCCC GCAAATGGAT GACCTATGTC 
CTGACCAAAT GTGAGAGTGG CAGGGGCACC AAGCCCAGGT GGGCGACTCA GGGGTTTGAC 
TTTCTTCAGG CTATCGAACC TGGCTTTATA TCAGCATTGC CAGAGGATGA CTTCTTGAAT 
TTGCAAGCTT TGATGAATGA ATGTATTGGA CATGTGATTG GAAAACCTCA TAGCCCAGTC 
ACCGGCCTGT ACATAGCTCC CAGAAACAGT CCTCGACCTG TAAAGGTGCC TCGCTGCCAT 
AGTGACCCAC CAAACCCCAA CCTGTTCATC CCTAATGCTG AAGGTTTCAG CTCTCGAAGT 
CTCCCCTGCG ACCTCCGGAA CCAGCTGTTC CCCAACGGCC CTCGCCCCGT CCCTCAGGGC 
CCGGGGGAAC AAAGCAACAC CAAAGCACCC AGCAGCAGCC CCAATGACGT CAGGGGATCC 
AGTTTCCATG AGAATGACCG CCTGTCGTCG GTTGCAGCAG AGTTGCATTT TAAATCTCTG 
AGCCGCCACT CCAGCCCCAC AGAGGACAGA GAAGAACCCT CTTATCCTAA GGGGGATCCT 
AACAGCACTG CACGCCGAAG CTGGGAGCTT CGCAACTTTA TCAGCCAGTC CAAGGACACA 
GCAGCAAGGC AAAGCCCCAT GGAGGCTGTC CGTCGCTCCA TTCGCAAGTT CGAGGACAAA 
CGCTATGCTG TGATGAAGCA GCGCAACATC ATCGGCCAAG TGTGTCACAC ACCCAAATCC 
TATGACAATG TCATGCATGT CGGGCTCAGG AAGGTGACCT TTAAGTGGCA GAGAGGCAAC 
AAGATAGGTG AGGGCCAGTA TGGGAAAGTG TACACCTGCA TCAATGTTGA CACTGGAGAA 
CTAATGGCCA TGAAGGAGAT CCGATTCCAG CCGAATGATC ACAAAACAAT CAAGGAGACT 
GCAGATGAGC TGAAAATATT TGAAGGCATT AAACACCCAA ACCTGGTGCG ATACTTTGGA 
GTGGAGCTCC ATCGGGAGGA GATGTACATC TTCATGGAGT ACTGTGACGA GGGCACCCTG 
GAGGAGGTGT CCAGACTGGG GCTGCAGGAA CATGTCATCA GGCTCTACAG TAAACAGATC 
ACTACAGCCA TCAATGTCCT CCATGAGCAT GGCATTGTCC ACCGCGATAT CAAGGGAGCC 
AACATCTTTT TGACATCGTC TGGCCTGATT AAGCTGGGTG ACTTTGGCTG TTCAGTGAAA 
TTGAGGAACA ACACACACAC CATGCCAGGG GAAGTGAACA GCACGCTGGG AACAGCAGCA 
TACATGGCAC CTGAAGTCAT CACCAGAGCA AAAGGGGAAG GTCACGGACG AGCGGCGGAC 
ATCTGGAGTT TGGGCTGTGT TCTCATTGAG ATGGTGACTG GAAAGAGGCC CTGGCACGAG 
TATGAGCACA ACTTCCAGAT CATGTACAAA GTGGGAATGG GCCATAAACC CCCCATCCCA 
GAGAAACTCA GCACAGAGGG GAAGGACTTC CTTGGTCACT GTCTGGAGAG TGAACCCAAA 
CGCCGCTGGA CAGCTAGCAT GCTGCTAGAC CACCCGTTTG TCAAGGTTTG TACGGATGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k4 ( XM_019275675.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019131220.2 Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OLa218136
Clone ID Related Accession (Same CDS sequence)	XM_019275675.2
Accession Version	XM_019275675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	mitogen-activated protein kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040013.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019275675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCGCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGTG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCACTA CTGACTCTGA CTGGGCTCAG TGGGATGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCCAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC GGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACCTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019131220.2
CDS	246..4892
Translation	MEPPDRNKPSRQAKPVQDASQQNSAVGESWDSPSEEEEALYGTSPPYNPRQMKRMSGKHQRSQARSAGRSPNKADLSPSGLRESPRPVETPEEHSYKQGKKQRATLRSTERDHKKTFEGAFMLDPLSKSTHFGSLNMDPRKHYLSLGCSSGKLPVSMPHMARTHRQTSRTDCPADRLKFFETLRLLLKLTSMSSKRKEKEQRGLENMAFMGHNNEVIWLELQAWHARRSTGDQDLFLFTARQAIPDIINEVLHFKVNYASLRGAQCSVEGEYQSVPDLVCGEEREPAVAETNHCGVDPWGFSAFPSSAMNAAEPLGSGAVCREHLQRQRLAFEQVKHVIELLESVEALYPSLQALQKDYEKYAARDFQGRVQALCLWLNITQDLNQKLRVMATVLGLHDLSRIGWPVFEIPSPRCSRGNEEEDNEEDEENDSTATFTAESDGEDRDAEEEEEEEEVVVLGHIAEGELSPSLTPKFARLLSEDEFLPTANAVSAEVIAGGGVFCPTAIYRPFVDKALKQMGLRKLILRLHKLMDRSLQRSRAALLRHTPALEFADFPDPMLYSDYLPELSRHESCSGPAHPELGADQVSWVDLLDMDLPSFRPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLRGVVTDAQGLQTNANIDEFEEDLHKMLVVYFDYMHSWIQMLQQLPQASHSLKNLLEEEWNFTKVITPYIRGGEAQSGKLFCDIAGMLLKSTGEFLDAGLQKSGNEFWETADDSTASDEIRRSVIETSRSLKELFHEARERASKALGFAKMLRKDLEVAADFSITNGVTCLLEALKKRNYVKVQIPGLEELQVFVPCGLMGQRPLILQLLNAAAGKDCSKEPDEIAEDEAYLLMSKHGAGDSTTDSDWAQWDGELLKLVPQMETVDTLRAMKVENVLLIVMQSAHLVAQRKAFQQSMEDVLTLSREQTSSQPLIASALEELKDEALQLCIKISTAIDRVEFMFTTEFEAEVEESESATLHQYYREAMIQGYNFAFEYHKEVVRLMSGEFRQRIGERYIAFARKWMTYVLTKCESGRGTKPRWATQGFDFLQAIEPGFISALPEDDFLNLQALMNECIGHVIGKPHSPVTGLYIAPRNSPRPVKVPRCHSDPPNPNLFIPNAEGFRGSSFHENDRLSSVAAELHFKSLSRHSSPTEDREEPSYPKGDPNSTARRSWELRNFISQSKDTAARQSPMEAVRRSIRKFEDKRYAVMKQRNIIGQVCHTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPEKLSTEGKDFLGHCLESEPKRRWTASMLLDHPFVKVCTDEE

Target ORF information:

RefSeq Version	XM_019275675.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea mitogen-activated protein kinase kinase kinase 4 (map3k4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019275675.2

ORF Insert Sequence:

ATGGAGCCAC CGGATCGTAA CAAGCCCAGC AGGCAGGCCA AGCCAGTGCA AGATGCATCC 
CAACAGAACT CTGCGGTGGG TGAATCCTGG GACTCTCCCA GCGAGGAAGA GGAAGCTCTG 
TATGGCACTT CACCTCCTTA TAACCCACGT CAGATGAAAC GCATGTCTGG CAAACATCAA 
AGGAGCCAAG CCAGGAGTGC TGGTCGGTCT CCTAACAAGG CAGACCTTAG CCCATCTGGC 
CTGAGAGAAA GTCCCAGGCC AGTGGAGACC CCTGAAGAGC ACAGCTACAA GCAGGGCAAG 
AAGCAGAGGG CCACACTGCG CTCCACGGAG AGAGACCACA AGAAGACCTT TGAAGGCGCC 
TTCATGCTGG ACCCACTCTC AAAGTCGACT CATTTCGGTT CCCTCAATAT GGATCCCAGG 
AAGCATTACT TGAGCTTGGG TTGCAGCAGT GGCAAACTTC CTGTGTCTAT GCCCCATATG 
GCTCGGACCC ACCGCCAGAC CTCCAGGACA GACTGTCCTG CTGACCGCCT CAAGTTCTTT 
GAAACTCTGC GGCTGCTCCT CAAGCTCACG TCTATGTCCT CCAAGAGGAA GGAGAAGGAG 
CAGAGAGGCC TGGAGAACAT GGCCTTCATG GGTCACAACA ACGAGGTCAT TTGGTTGGAG 
CTGCAGGCTT GGCATGCACG GCGCTCCACC GGCGACCAAG ACCTTTTTCT TTTTACTGCC 
CGTCAAGCCA TCCCTGACAT CATCAATGAG GTGCTGCACT TTAAGGTCAA CTATGCCAGC 
CTGAGAGGGG CCCAGTGTTC AGTCGAGGGG GAATATCAGA GTGTCCCAGA TCTAGTCTGT 
GGAGAGGAGA GAGAGCCTGC CGTAGCAGAG ACCAACCACT GTGGAGTAGA TCCCTGGGGC 
TTTAGTGCCT TCCCCTCATC AGCAATGAAT GCAGCAGAGC CTCTGGGCTC TGGCGCTGTT 
TGCAGGGAGC ATCTTCAGCG CCAGCGGCTG GCATTTGAGC AGGTCAAACA TGTTATAGAG 
TTGCTGGAGT CAGTGGAGGC TTTGTACCCC TCTTTGCAGG CCCTGCAGAA GGACTATGAA 
AAGTATGCAG CACGAGATTT CCAGGGCAGG GTGCAGGCGC TCTGTCTGTG GCTCAATATC 
ACCCAGGACC TCAACCAGAA GCTGCGTGTT ATGGCAACTG TGCTGGGCCT CCATGATCTA 
TCTCGTATCG GGTGGCCTGT CTTTGAAATC CCCTCACCTC GCTGTTCCCG TGGCAATGAA 
GAAGAGGACA ATGAGGAAGA CGAGGAGAAT GACTCAACAG CCACTTTTAC AGCTGAAAGT 
GATGGAGAGG ACAGGGATGC TGAGGAGGAG GAGGAGGAGG AGGAGGTGGT AGTATTGGGT 
CACATAGCAG AGGGAGAGTT GTCTCCCTCC CTGACTCCTA AATTTGCCCG ATTGTTGTCA 
GAGGACGAGT TTCTCCCAAC TGCTAACGCA GTAAGTGCAG AAGTGATTGC GGGAGGGGGA 
GTATTCTGCC CCACAGCCAT CTACAGACCG TTTGTGGATA AAGCTCTGAA GCAGATGGGG 
CTGCGTAAAC TGATCCTCAG ACTGCACAAA CTGATGGACC GCTCTCTTCA GAGGTCCAGA 
GCAGCACTGC TCCGTCACAC TCCTGCACTG GAGTTTGCAG ATTTCCCTGA CCCCATGCTG 
TACAGTGATT ACCTGCCAGA GCTATCGCGC CATGAGTCCT GCAGTGGTCC GGCACATCCG 
GAGCTTGGGG CAGACCAGGT TTCCTGGGTG GATTTGCTGG ACATGGATCT CCCGTCCTTT 
CGGCCAGCGT TCCTTGTGCT GTGCAGAGTC CTTCTAAACG TCATTCACGA ATGCCTTAAA 
CTGCGACTTG AACAGAGGCC TGCTGGGGAG CCTTCACTGC TCAGCATCAA ACAGTTGGTG 
CGTGAGTGTA AGGAGGTTCT TAAAGGTGGT CTTCTGATGA AGCAGTATTA TCAGTTCATG 
CTGCGAGGCG TGGTGACTGA TGCTCAGGGC TTGCAGACGA ATGCCAACAT TGATGAGTTT 
GAAGAGGATC TTCACAAGAT GCTGGTGGTC TACTTTGATT ACATGCACAG TTGGATCCAG 
ATGTTGCAGC AGCTGCCTCA GGCCTCTCAC AGCTTAAAGA ACCTGTTGGA GGAGGAGTGG 
AACTTCACCA AGGTCATCAC TCCTTACATT CGAGGAGGGG AGGCCCAGTC CGGGAAGCTT 
TTTTGTGACA TCGCTGGGAT GCTGCTTAAA TCCACTGGAG AGTTCCTTGA TGCCGGCCTG 
CAAAAGAGCG GTAATGAATT CTGGGAAACT GCAGATGACA GCACCGCCTC AGATGAGATC 
AGGCGGTCAG TTATCGAGAC TAGTCGGTCG CTCAAAGAGC TGTTCCACGA GGCCAGGGAA 
CGAGCGTCCA AGGCTCTCGG CTTTGCCAAG ATGCTTCGCA AGGACTTGGA AGTTGCTGCA 
GATTTCAGCA TCACTAATGG GGTGACTTGT CTCCTGGAGG CACTGAAGAA GAGAAACTAT 
GTCAAGGTTC AGATTCCAGG CTTGGAGGAG CTGCAGGTTT TTGTCCCTTG TGGCTTGATG 
GGTCAGCGGC CTCTCATCCT GCAGCTTCTC AATGCTGCTG CTGGCAAAGA CTGCTCCAAG 
GAGCCTGATG AAATTGCAGA GGACGAGGCC TACCTGCTTA TGAGTAAACA CGGAGCTGGG 
GATTCCACTA CTGACTCTGA CTGGGCTCAG TGGGATGGAG AGCTGCTCAA ACTGGTTCCC 
CAGATGGAAA CTGTTGACAC TTTAAGGGCC ATGAAGGTAG AGAATGTGCT CTTGATTGTG 
ATGCAGTCTG CTCACCTGGT GGCCCAGCGA AAGGCCTTTC AGCAGTCCAT GGAGGATGTG 
CTCACTCTTA GCCGGGAGCA GACATCTAGT CAGCCACTCA TTGCGAGTGC GCTGGAGGAA 
CTCAAGGATG AGGCACTACA GCTATGCATA AAGATCAGCA CTGCCATTGA CCGAGTGGAG 
TTCATGTTCA CCACAGAGTT TGAGGCAGAG GTGGAGGAGT CTGAGTCAGC CACTCTGCAT 
CAGTACTACA GGGAGGCAAT GATACAGGGC TACAATTTTG CCTTCGAGTA CCACAAGGAG 
GTGGTGCGGC TAATGTCAGG GGAGTTCAGA CAAAGGATCG GGGAGCGCTA CATTGCTTTT 
GCCCGCAAAT GGATGACCTA TGTCCTGACC AAATGTGAGA GTGGCAGGGG CACCAAGCCC 
AGGTGGGCGA CTCAGGGGTT TGACTTTCTT CAGGCTATCG AACCTGGCTT TATATCAGCA 
TTGCCAGAGG ATGACTTCTT GAATTTGCAA GCTTTGATGA ATGAATGTAT TGGACATGTG 
ATTGGAAAAC CTCATAGCCC AGTCACCGGC CTGTACATAG CTCCCAGAAA CAGTCCTCGA 
CCTGTAAAGG TGCCTCGCTG CCATAGTGAC CCACCAAACC CCAACCTGTT CATCCCTAAT 
GCTGAAGGTT TCAGGGGATC CAGTTTCCAT GAGAATGACC GCCTGTCGTC GGTTGCAGCA 
GAGTTGCATT TTAAATCTCT GAGCCGCCAC TCCAGCCCCA CAGAGGACAG AGAAGAACCC 
TCTTATCCTA AGGGGGATCC TAACAGCACT GCACGCCGAA GCTGGGAGCT TCGCAACTTT 
ATCAGCCAGT CCAAGGACAC AGCAGCAAGG CAAAGCCCCA TGGAGGCTGT CCGTCGCTCC 
ATTCGCAAGT TCGAGGACAA ACGCTATGCT GTGATGAAGC AGCGCAACAT CATCGGCCAA 
GTGTGTCACA CACCCAAATC CTATGACAAT GTCATGCATG TCGGGCTCAG GAAGGTGACC 
TTTAAGTGGC AGAGAGGCAA CAAGATAGGT GAGGGCCAGT ATGGGAAAGT GTACACCTGC 
ATCAATGTTG ACACTGGAGA ACTAATGGCC ATGAAGGAGA TCCGATTCCA GCCGAATGAT 
CACAAAACAA TCAAGGAGAC TGCAGATGAG CTGAAAATAT TTGAAGGCAT TAAACACCCA 
AACCTGGTGC GATACTTTGG AGTGGAGCTC CATCGGGAGG AGATGTACAT CTTCATGGAG 
TACTGTGACG AGGGCACCCT GGAGGAGGTG TCCAGACTGG GGCTGCAGGA ACATGTCATC 
AGGCTCTACA GTAAACAGAT CACTACAGCC ATCAATGTCC TCCATGAGCA TGGCATTGTC 
CACCGCGATA TCAAGGGAGC CAACATCTTT TTGACATCGT CTGGCCTGAT TAAGCTGGGT 
GACTTTGGCT GTTCAGTGAA ATTGAGGAAC AACACACACA CCATGCCAGG GGAAGTGAAC 
AGCACGCTGG GAACAGCAGC ATACATGGCA CCTGAAGTCA TCACCAGAGC AAAAGGGGAA 
GGTCACGGAC GAGCGGCGGA CATCTGGAGT TTGGGCTGTG TTCTCATTGA GATGGTGACT 
GGAAAGAGGC CCTGGCACGA GTATGAGCAC AACTTCCAGA TCATGTACAA AGTGGGAATG 
GGCCATAAAC CCCCCATCCC AGAGAAACTC AGCACAGAGG GGAAGGACTT CCTTGGTCAC 
TGTCTGGAGA GTGAACCCAA ACGCCGCTGG ACAGCTAGCA TGCTGCTAGA CCACCCGTTT 
GTCAAGGTTT GTACGGATGA AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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03-6811-6573
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オンライン注文

map3k4 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム