nek9 cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

Gene
Clones
gRNAs

Summary

Gene Symbol	nek9
Entrez Gene ID	108237350
Full Name	NIMA related kinase 9
Gene Type	protein-coding
Organism	Kryptolebias marmoratus(mangrove rivulus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017418711.2	XP_017274200.1	serine/threonine-protein kinase Nek9
XM_017418711.1	XP_017274200.1	serine/threonine-protein kinase Nek9

Pathways from BioSystems
Gene Ontology

The following nek9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nek9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OKr01391	XM_017418711.2 Latest version!	Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OKr01391	XM_017418711.1	Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nek9 ( XM_017418711.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017274200.1 Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.

Price & Availability↑

CloneID	OKr01391
Clone ID Related Accession (Same CDS sequence)	XM_017418711.2 , XM_017418711.1
Accession Version	XM_017418711.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	serine/threonine-protein kinase Nek9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094241.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017418711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG ATGAGTACGA GAGGCATTTC GACTCGCTGA ACTCGGATTT GGGCGGCGGG 
GCGGTCAGCG AGCGGTCAGC TTCGGGTACG TTTAATGGCG AAGAGGAGAA GCTGCATTAC 
ATTCCCATCC GGATCCTCGG GAGGGGGGCG TTCGGCGAGG CTACTCTGTA CAGAAGAACA 
GAAGACAACT CCCTAGTTGT GTGGAAGGAG GTGGACCTGA ACTCGCTCTC GGAGAAGGAG 
CACCGGGATG TCATGAATGA AATCAGCATC CTCTCCATGC TGGAGCACAA CAACATCATC 
GCCTACTTCA ATCACTTCAT GGATAAAAAC ACTCTGCTCA TTGAGCTGGA GTATTGCAAT 
GGAGGAAATC TGTATGATAA AATTGTTCAG CAGAGGGGGA AGCTTTTCCC TCAGGAGATT 
GTTATATGGT ACCTGTATCA GATCACCTCA GCGGTGGCCC ACATTCACAA GGCTGGAGTC 
ATACACAGAG ATATCAAAAC TCTGAACATT TTCCTGACCA AGACCAACCT CATTAAGCTG 
GGCGACTATG GCCTTGCGAA GAAGCTAGGC TCTGAGTTTT CGATGGCAGA GACTTGTGTA 
GGAACTCCGT ATTACATGTC ACCTGAACTG TGTCAGGGAG ACAAGTACAA CTTCAAATCA 
GACATCTGGG CTGTGGGTTG TGTACTTTTT GAGGTCCTAG TGCTTAAAAG AGCCTTTGAT 
GCAACGAACC GTCTAAACCT CTGTGTGAAA ATAGTCCAGG GAAACTGGAC TATGGACGTG 
AACTCGGACG TTTATTCAAG TGGATTGATC AAGTTGGTGT GTGATTGTCT CAATCAGGAT 
CCCGCAAAGA GGCCGACAGC TGAGGAGATC CTGGATCAGC CTTTTATCTC CTGCTGCCGA 
CAGGAGCTGG AGGAGCAAGT TGTCATGCTT AACTCGGCGA TGAAGAAACC AAAGCTGAGC 
ACAGTGACCG AGACTCCTGT TGCTGTGGTG ACGACGCGCT CGAGGGAGGT GTATTTTTGG 
GGAGGAGGAA AATTTACCCC CCAGAAGCTT GACTCGTTCA AAGGAGGCAG CAGCGCTCAA 
CACGTTTGTG CCGGGGAGAG CCACTTTGCG GTGGTGACGG TGGAGAAGGA GCTGTTTACT 
TGGGCTAATG TGCAAGGTGG GGCCAAGATG GTGGGCCAGC TGGGGCACGG CGACCAGGCC 
TCTTACCGGC AGCCGAAGAG GGTGGAGAAG CTTCAGGGAA AGGCGATCCG GCAGGTGGCA 
TGTGGGGCCG ACTTCACTGC CTGTGTGACC GACGAAGACC AGCTCTACAT GTTCGGGTCA 
GACTACTACG GCTGCATCGG AGTTGAGGGC GAGATGGGGA TGGAGATTTT GGAGCCTGTT 
CTTCTGGAGT TTTTTGAGGA GCGGTGTGTC TACCAGGTCT CCTGTGGAGA CAACCACGTT 
GTGGTGCTGA CTCAGGATGG CAGCATTTTC TCTTGGGGCT GTGGAGAATA TGGACGTCTT 
GGCCTGGAGT GTGAAGATGA CTTCTCTTCT CCAATGCAAG TGGAAATTCC TAGAGGAGCC 
ACCATCTCCT CTGTGTCGTG TGGCTGTGAT GGGACCTTCT TTCTGACAGA GACTGGGAAA 
GTCTTAGCAT GTGGAAACAA TGAGTACAAC AAGCTGGGGC TGAACCAGGG AATCTCTGGT 
CTCAAAAACC ACCCTGAAAA GGGTTACCAG GGAATACCGT ACATCACCAC GCTGACCTTG 
GTTAAACAGC TGTCGCGCTT CAAGATCCAA GTCATAGCTC CAGGGAAGAC TCACACAGCC 
GCCATTGATG GACGAGGTCG TCTGATCACT TTCGGTTGCA ACAAGTATGG GCAGCTGGGT 
GTGAAGGACT TCAAGAAGCA CCAAGGTGTT CAAGTTCTTG TTGGACCATT CGGAGGGAAG 
ACTGTGACCA AAGTGTCCTG TGGAGATGGA TTCACCATTG CAGCCACTGA GGACAATCAT 
ATCTTTGCAT GGGGAAATGC AGGAAACGGG AGACTTGGAA TGCCCGCAGA TAAAGGATTC 
GGTTCAGAGG TTTGCCCTGC CATGCCGAGG CCCATATTTG GTTCCCTCCA CCACGTGCCT 
GATCTCTCCT GCCGCGGCTG GCACACCATT ATCATAATGG AAAAAGTGCT GAATTCAAAA 
ACCATCCGCT CAAACAGCAG TGGACTATCA ATCGGGCTTG GCCAGGAGGC ATCTACGTCC 
ACGGTGGAGC TGGACATAGA GCCTGGACTG GAGACGGACT GTCGCGACGG CAGTCTTGGG 
GGAACACGGG AGATTAACAC AGAGGAGGGT GTCATGGACA CGTCGATGAT GTCATTCGGA 
AATCAGACGG GGGACAGTTC CTGTCCCCTG TGGCTCAAAA AGGAGCTTGA GGACGCAGAG 
TTCATCCCGA TGCCAGAGGA GTCCGACCAG TCCACTCACG ATCAGCTCTC CTCGATCTCC 
GAGAGCGTCA CTCGGCCATA CGAGGAGCTG AAAGAGCTCA AGGCAGCGGC CGAGGCAGTC 
AGCAGTCAGA AAGGCCTATC GCAAACCAGG CAAATGAGCT TTGAGAAGGT CAGAGGGCTG 
GATGACGACG ACGATGACGT CTTTCCAAAA AAAGAATCCG GTGCGTGCTG CAGTGCAAGT 
ATTGAAGTGG CTCAGCTGAG AGAAACGGTT ACCCGTCAAG AGTCGAGGAT CGAGATGCTT 
GAAAAACAGG TCAGTGAGCA GCAGCGGGAG AACGAAAGGC TCTGGGCAGC GATTAATCGT 
TCAGCGCAGC AGGAAGCAGG ACGTGACGGC AACAGGAATC ATGTCTCTGT GGCGAGCAGT 
GGAGGAGGTG AAAGAAGGCT GACGAACCAC GGAGGCAGAT CTGCTGGAGC AAGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017274200.1
CDS	733..3612
Translation	MSLDEYERHFDSLNSDLGGGAVSERSASGTFNGEEEKLHYIPIRILGRGAFGEATLYRRTEDNSLVVWKEVDLNSLSEKEHRDVMNEISILSMLEHNNIIAYFNHFMDKNTLLIELEYCNGGNLYDKIVQQRGKLFPQEIVIWYLYQITSAVAHIHKAGVIHRDIKTLNIFLTKTNLIKLGDYGLAKKLGSEFSMAETCVGTPYYMSPELCQGDKYNFKSDIWAVGCVLFEVLVLKRAFDATNRLNLCVKIVQGNWTMDVNSDVYSSGLIKLVCDCLNQDPAKRPTAEEILDQPFISCCRQELEEQVVMLNSAMKKPKLSTVTETPVAVVTTRSREVYFWGGGKFTPQKLDSFKGGSSAQHVCAGESHFAVVTVEKELFTWANVQGGAKMVGQLGHGDQASYRQPKRVEKLQGKAIRQVACGADFTACVTDEDQLYMFGSDYYGCIGVEGEMGMEILEPVLLEFFEERCVYQVSCGDNHVVVLTQDGSIFSWGCGEYGRLGLECEDDFSSPMQVEIPRGATISSVSCGCDGTFFLTETGKVLACGNNEYNKLGLNQGISGLKNHPEKGYQGIPYITTLTLVKQLSRFKIQVIAPGKTHTAAIDGRGRLITFGCNKYGQLGVKDFKKHQGVQVLVGPFGGKTVTKVSCGDGFTIAATEDNHIFAWGNAGNGRLGMPADKGFGSEVCPAMPRPIFGSLHHVPDLSCRGWHTIIIMEKVLNSKTIRSNSSGLSIGLGQEASTSTVELDIEPGLETDCRDGSLGGTREINTEEGVMDTSMMSFGNQTGDSSCPLWLKKELEDAEFIPMPEESDQSTHDQLSSISESVTRPYEELKELKAAAEAVSSQKGLSQTRQMSFEKVRGLDDDDDDVFPKKESGACCSASIEVAQLRETVTRQESRIEMLEKQVSEQQRENERLWAAINRSAQQEAGRDGNRNHVSVASSGGGERRLTNHGGRSAGASA

Target ORF information:

RefSeq Version	XM_017418711.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017418711.2

ORF Insert Sequence:

ATGTCTCTGG ATGAGTACGA GAGGCATTTC GACTCGCTGA ACTCGGATTT GGGCGGCGGG 
GCGGTCAGCG AGCGGTCAGC TTCGGGTACG TTTAATGGCG AAGAGGAGAA GCTGCATTAC 
ATTCCCATCC GGATCCTCGG GAGGGGGGCG TTCGGCGAGG CTACTCTGTA CAGAAGAACA 
GAAGACAACT CCCTAGTTGT GTGGAAGGAG GTGGACCTGA ACTCGCTCTC GGAGAAGGAG 
CACCGGGATG TCATGAATGA AATCAGCATC CTCTCCATGC TGGAGCACAA CAACATCATC 
GCCTACTTCA ATCACTTCAT GGATAAAAAC ACTCTGCTCA TTGAGCTGGA GTATTGCAAT 
GGAGGAAATC TGTATGATAA AATTGTTCAG CAGAGGGGGA AGCTTTTCCC TCAGGAGATT 
GTTATATGGT ACCTGTATCA GATCACCTCA GCGGTGGCCC ACATTCACAA GGCTGGAGTC 
ATACACAGAG ATATCAAAAC TCTGAACATT TTCCTGACCA AGACCAACCT CATTAAGCTG 
GGCGACTATG GCCTTGCGAA GAAGCTAGGC TCTGAGTTTT CGATGGCAGA GACTTGTGTA 
GGAACTCCGT ATTACATGTC ACCTGAACTG TGTCAGGGAG ACAAGTACAA CTTCAAATCA 
GACATCTGGG CTGTGGGTTG TGTACTTTTT GAGGTCCTAG TGCTTAAAAG AGCCTTTGAT 
GCAACGAACC GTCTAAACCT CTGTGTGAAA ATAGTCCAGG GAAACTGGAC TATGGACGTG 
AACTCGGACG TTTATTCAAG TGGATTGATC AAGTTGGTGT GTGATTGTCT CAATCAGGAT 
CCCGCAAAGA GGCCGACAGC TGAGGAGATC CTGGATCAGC CTTTTATCTC CTGCTGCCGA 
CAGGAGCTGG AGGAGCAAGT TGTCATGCTT AACTCGGCGA TGAAGAAACC AAAGCTGAGC 
ACAGTGACCG AGACTCCTGT TGCTGTGGTG ACGACGCGCT CGAGGGAGGT GTATTTTTGG 
GGAGGAGGAA AATTTACCCC CCAGAAGCTT GACTCGTTCA AAGGAGGCAG CAGCGCTCAA 
CACGTTTGTG CCGGGGAGAG CCACTTTGCG GTGGTGACGG TGGAGAAGGA GCTGTTTACT 
TGGGCTAATG TGCAAGGTGG GGCCAAGATG GTGGGCCAGC TGGGGCACGG CGACCAGGCC 
TCTTACCGGC AGCCGAAGAG GGTGGAGAAG CTTCAGGGAA AGGCGATCCG GCAGGTGGCA 
TGTGGGGCCG ACTTCACTGC CTGTGTGACC GACGAAGACC AGCTCTACAT GTTCGGGTCA 
GACTACTACG GCTGCATCGG AGTTGAGGGC GAGATGGGGA TGGAGATTTT GGAGCCTGTT 
CTTCTGGAGT TTTTTGAGGA GCGGTGTGTC TACCAGGTCT CCTGTGGAGA CAACCACGTT 
GTGGTGCTGA CTCAGGATGG CAGCATTTTC TCTTGGGGCT GTGGAGAATA TGGACGTCTT 
GGCCTGGAGT GTGAAGATGA CTTCTCTTCT CCAATGCAAG TGGAAATTCC TAGAGGAGCC 
ACCATCTCCT CTGTGTCGTG TGGCTGTGAT GGGACCTTCT TTCTGACAGA GACTGGGAAA 
GTCTTAGCAT GTGGAAACAA TGAGTACAAC AAGCTGGGGC TGAACCAGGG AATCTCTGGT 
CTCAAAAACC ACCCTGAAAA GGGTTACCAG GGAATACCGT ACATCACCAC GCTGACCTTG 
GTTAAACAGC TGTCGCGCTT CAAGATCCAA GTCATAGCTC CAGGGAAGAC TCACACAGCC 
GCCATTGATG GACGAGGTCG TCTGATCACT TTCGGTTGCA ACAAGTATGG GCAGCTGGGT 
GTGAAGGACT TCAAGAAGCA CCAAGGTGTT CAAGTTCTTG TTGGACCATT CGGAGGGAAG 
ACTGTGACCA AAGTGTCCTG TGGAGATGGA TTCACCATTG CAGCCACTGA GGACAATCAT 
ATCTTTGCAT GGGGAAATGC AGGAAACGGG AGACTTGGAA TGCCCGCAGA TAAAGGATTC 
GGTTCAGAGG TTTGCCCTGC CATGCCGAGG CCCATATTTG GTTCCCTCCA CCACGTGCCT 
GATCTCTCCT GCCGCGGCTG GCACACCATT ATCATAATGG AAAAAGTGCT GAATTCAAAA 
ACCATCCGCT CAAACAGCAG TGGACTATCA ATCGGGCTTG GCCAGGAGGC ATCTACGTCC 
ACGGTGGAGC TGGACATAGA GCCTGGACTG GAGACGGACT GTCGCGACGG CAGTCTTGGG 
GGAACACGGG AGATTAACAC AGAGGAGGGT GTCATGGACA CGTCGATGAT GTCATTCGGA 
AATCAGACGG GGGACAGTTC CTGTCCCCTG TGGCTCAAAA AGGAGCTTGA GGACGCAGAG 
TTCATCCCGA TGCCAGAGGA GTCCGACCAG TCCACTCACG ATCAGCTCTC CTCGATCTCC 
GAGAGCGTCA CTCGGCCATA CGAGGAGCTG AAAGAGCTCA AGGCAGCGGC CGAGGCAGTC 
AGCAGTCAGA AAGGCCTATC GCAAACCAGG CAAATGAGCT TTGAGAAGGT CAGAGGGCTG 
GATGACGACG ACGATGACGT CTTTCCAAAA AAAGAATCCG GTGCGTGCTG CAGTGCAAGT 
ATTGAAGTGG CTCAGCTGAG AGAAACGGTT ACCCGTCAAG AGTCGAGGAT CGAGATGCTT 
GAAAAACAGG TCAGTGAGCA GCAGCGGGAG AACGAAAGGC TCTGGGCAGC GATTAATCGT 
TCAGCGCAGC AGGAAGCAGG ACGTGACGGC AACAGGAATC ATGTCTCTGT GGCGAGCAGT 
GGAGGAGGTG AAAGAAGGCT GACGAACCAC GGAGGCAGAT CTGCTGGAGC AAGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nek9 ( XM_017418711.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017274200.1 Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.

Price & Availability↑

CloneID	OKr01391
Clone ID Related Accession (Same CDS sequence)	XM_017418711.2 , XM_017418711.1
Accession Version	XM_017418711.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	serine/threonine-protein kinase Nek9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094241.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG ATGAGTACGA GAGGCATTTC GACTCGCTGA ACTCGGATTT GGGCGGCGGG 
GCGGTCAGCG AGCGGTCAGC TTCGGGTACG TTTAATGGCG AAGAGGAGAA GCTGCATTAC 
ATTCCCATCC GGATCCTCGG GAGGGGGGCG TTCGGCGAGG CTACTCTGTA CAGAAGAACA 
GAAGACAACT CCCTAGTTGT GTGGAAGGAG GTGGACCTGA ACTCGCTCTC GGAGAAGGAG 
CACCGGGATG TCATGAATGA AATCAGCATC CTCTCCATGC TGGAGCACAA CAACATCATC 
GCCTACTTCA ATCACTTCAT GGATAAAAAC ACTCTGCTCA TTGAGCTGGA GTATTGCAAT 
GGAGGAAATC TGTATGATAA AATTGTTCAG CAGAGGGGGA AGCTTTTCCC TCAGGAGATT 
GTTATATGGT ACCTGTATCA GATCACCTCA GCGGTGGCCC ACATTCACAA GGCTGGAGTC 
ATACACAGAG ATATCAAAAC TCTGAACATT TTCCTGACCA AGACCAACCT CATTAAGCTG 
GGCGACTATG GCCTTGCGAA GAAGCTAGGC TCTGAGTTTT CGATGGCAGA GACTTGTGTA 
GGAACTCCGT ATTACATGTC ACCTGAACTG TGTCAGGGAG ACAAGTACAA CTTCAAATCA 
GACATCTGGG CTGTGGGTTG TGTACTTTTT GAGGTCCTAG TGCTTAAAAG AGCCTTTGAT 
GCAACGAACC GTCTAAACCT CTGTGTGAAA ATAGTCCAGG GAAACTGGAC TATGGACGTG 
AACTCGGACG TTTATTCAAG TGGATTGATC AAGTTGGTGT GTGATTGTCT CAATCAGGAT 
CCCGCAAAGA GGCCGACAGC TGAGGAGATC CTGGATCAGC CTTTTATCTC CTGCTGCCGA 
CAGGAGCTGG AGGAGCAAGT TGTCATGCTT AACTCGGCGA TGAAGAAACC AAAGCTGAGC 
ACAGTGACCG AGACTCCTGT TGCTGTGGTG ACGACGCGCT CGAGGGAGGT GTATTTTTGG 
GGAGGAGGAA AATTTACCCC CCAGAAGCTT GACTCGTTCA AAGGAGGCAG CAGCGCTCAA 
CACGTTTGTG CCGGGGAGAG CCACTTTGCG GTGGTGACGG TGGAGAAGGA GCTGTTTACT 
TGGGCTAATG TGCAAGGTGG GGCCAAGATG GTGGGCCAGC TGGGGCACGG CGACCAGGCC 
TCTTACCGGC AGCCGAAGAG GGTGGAGAAG CTTCAGGGAA AGGCGATCCG GCAGGTGGCA 
TGTGGGGCCG ACTTCACTGC CTGTGTGACC GACGAAGACC AGCTCTACAT GTTCGGGTCA 
GACTACTACG GCTGCATCGG AGTTGAGGGC GAGATGGGGA TGGAGATTTT GGAGCCTGTT 
CTTCTGGAGT TTTTTGAGGA GCGGTGTGTC TACCAGGTCT CCTGTGGAGA CAACCACGTT 
GTGGTGCTGA CTCAGGATGG CAGCATTTTC TCTTGGGGCT GTGGAGAATA TGGACGTCTT 
GGCCTGGAGT GTGAAGATGA CTTCTCTTCT CCAATGCAAG TGGAAATTCC TAGAGGAGCC 
ACCATCTCCT CTGTGTCGTG TGGCTGTGAT GGGACCTTCT TTCTGACAGA GACTGGGAAA 
GTCTTAGCAT GTGGAAACAA TGAGTACAAC AAGCTGGGGC TGAACCAGGG AATCTCTGGT 
CTCAAAAACC ACCCTGAAAA GGGTTACCAG GGAATACCGT ACATCACCAC GCTGACCTTG 
GTTAAACAGC TGTCGCGCTT CAAGATCCAA GTCATAGCTC CAGGGAAGAC TCACACAGCC 
GCCATTGATG GACGAGGTCG TCTGATCACT TTCGGTTGCA ACAAGTATGG GCAGCTGGGT 
GTGAAGGACT TCAAGAAGCA CCAAGGTGTT CAAGTTCTTG TTGGACCATT CGGAGGGAAG 
ACTGTGACCA AAGTGTCCTG TGGAGATGGA TTCACCATTG CAGCCACTGA GGACAATCAT 
ATCTTTGCAT GGGGAAATGC AGGAAACGGG AGACTTGGAA TGCCCGCAGA TAAAGGATTC 
GGTTCAGAGG TTTGCCCTGC CATGCCGAGG CCCATATTTG GTTCCCTCCA CCACGTGCCT 
GATCTCTCCT GCCGCGGCTG GCACACCATT ATCATAATGG AAAAAGTGCT GAATTCAAAA 
ACCATCCGCT CAAACAGCAG TGGACTATCA ATCGGGCTTG GCCAGGAGGC ATCTACGTCC 
ACGGTGGAGC TGGACATAGA GCCTGGACTG GAGACGGACT GTCGCGACGG CAGTCTTGGG 
GGAACACGGG AGATTAACAC AGAGGAGGGT GTCATGGACA CGTCGATGAT GTCATTCGGA 
AATCAGACGG GGGACAGTTC CTGTCCCCTG TGGCTCAAAA AGGAGCTTGA GGACGCAGAG 
TTCATCCCGA TGCCAGAGGA GTCCGACCAG TCCACTCACG ATCAGCTCTC CTCGATCTCC 
GAGAGCGTCA CTCGGCCATA CGAGGAGCTG AAAGAGCTCA AGGCAGCGGC CGAGGCAGTC 
AGCAGTCAGA AAGGCCTATC GCAAACCAGG CAAATGAGCT TTGAGAAGGT CAGAGGGCTG 
GATGACGACG ACGATGACGT CTTTCCAAAA AAAGAATCCG GTGCGTGCTG CAGTGCAAGT 
ATTGAAGTGG CTCAGCTGAG AGAAACGGTT ACCCGTCAAG AGTCGAGGAT CGAGATGCTT 
GAAAAACAGG TCAGTGAGCA GCAGCGGGAG AACGAAAGGC TCTGGGCAGC GATTAATCGT 
TCAGCGCAGC AGGAAGCAGG ACGTGACGGC AACAGGAATC ATGTCTCTGT GGCGAGCAGT 
GGAGGAGGTG AAAGAAGGCT GACGAACCAC GGAGGCAGAT CTGCTGGAGC AAGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017274200.1
CDS	672..3551
Translation	MSLDEYERHFDSLNSDLGGGAVSERSASGTFNGEEEKLHYIPIRILGRGAFGEATLYRRTEDNSLVVWKEVDLNSLSEKEHRDVMNEISILSMLEHNNIIAYFNHFMDKNTLLIELEYCNGGNLYDKIVQQRGKLFPQEIVIWYLYQITSAVAHIHKAGVIHRDIKTLNIFLTKTNLIKLGDYGLAKKLGSEFSMAETCVGTPYYMSPELCQGDKYNFKSDIWAVGCVLFEVLVLKRAFDATNRLNLCVKIVQGNWTMDVNSDVYSSGLIKLVCDCLNQDPAKRPTAEEILDQPFISCCRQELEEQVVMLNSAMKKPKLSTVTETPVAVVTTRSREVYFWGGGKFTPQKLDSFKGGSSAQHVCAGESHFAVVTVEKELFTWANVQGGAKMVGQLGHGDQASYRQPKRVEKLQGKAIRQVACGADFTACVTDEDQLYMFGSDYYGCIGVEGEMGMEILEPVLLEFFEERCVYQVSCGDNHVVVLTQDGSIFSWGCGEYGRLGLECEDDFSSPMQVEIPRGATISSVSCGCDGTFFLTETGKVLACGNNEYNKLGLNQGISGLKNHPEKGYQGIPYITTLTLVKQLSRFKIQVIAPGKTHTAAIDGRGRLITFGCNKYGQLGVKDFKKHQGVQVLVGPFGGKTVTKVSCGDGFTIAATEDNHIFAWGNAGNGRLGMPADKGFGSEVCPAMPRPIFGSLHHVPDLSCRGWHTIIIMEKVLNSKTIRSNSSGLSIGLGQEASTSTVELDIEPGLETDCRDGSLGGTREINTEEGVMDTSMMSFGNQTGDSSCPLWLKKELEDAEFIPMPEESDQSTHDQLSSISESVTRPYEELKELKAAAEAVSSQKGLSQTRQMSFEKVRGLDDDDDDVFPKKESGACCSASIEVAQLRETVTRQESRIEMLEKQVSEQQRENERLWAAINRSAQQEAGRDGNRNHVSVASSGGGERRLTNHGGRSAGASA

Target ORF information:

RefSeq Version	XM_017418711.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus NIMA related kinase 9 (nek9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017418711.1

ORF Insert Sequence:

ATGTCTCTGG ATGAGTACGA GAGGCATTTC GACTCGCTGA ACTCGGATTT GGGCGGCGGG 
GCGGTCAGCG AGCGGTCAGC TTCGGGTACG TTTAATGGCG AAGAGGAGAA GCTGCATTAC 
ATTCCCATCC GGATCCTCGG GAGGGGGGCG TTCGGCGAGG CTACTCTGTA CAGAAGAACA 
GAAGACAACT CCCTAGTTGT GTGGAAGGAG GTGGACCTGA ACTCGCTCTC GGAGAAGGAG 
CACCGGGATG TCATGAATGA AATCAGCATC CTCTCCATGC TGGAGCACAA CAACATCATC 
GCCTACTTCA ATCACTTCAT GGATAAAAAC ACTCTGCTCA TTGAGCTGGA GTATTGCAAT 
GGAGGAAATC TGTATGATAA AATTGTTCAG CAGAGGGGGA AGCTTTTCCC TCAGGAGATT 
GTTATATGGT ACCTGTATCA GATCACCTCA GCGGTGGCCC ACATTCACAA GGCTGGAGTC 
ATACACAGAG ATATCAAAAC TCTGAACATT TTCCTGACCA AGACCAACCT CATTAAGCTG 
GGCGACTATG GCCTTGCGAA GAAGCTAGGC TCTGAGTTTT CGATGGCAGA GACTTGTGTA 
GGAACTCCGT ATTACATGTC ACCTGAACTG TGTCAGGGAG ACAAGTACAA CTTCAAATCA 
GACATCTGGG CTGTGGGTTG TGTACTTTTT GAGGTCCTAG TGCTTAAAAG AGCCTTTGAT 
GCAACGAACC GTCTAAACCT CTGTGTGAAA ATAGTCCAGG GAAACTGGAC TATGGACGTG 
AACTCGGACG TTTATTCAAG TGGATTGATC AAGTTGGTGT GTGATTGTCT CAATCAGGAT 
CCCGCAAAGA GGCCGACAGC TGAGGAGATC CTGGATCAGC CTTTTATCTC CTGCTGCCGA 
CAGGAGCTGG AGGAGCAAGT TGTCATGCTT AACTCGGCGA TGAAGAAACC AAAGCTGAGC 
ACAGTGACCG AGACTCCTGT TGCTGTGGTG ACGACGCGCT CGAGGGAGGT GTATTTTTGG 
GGAGGAGGAA AATTTACCCC CCAGAAGCTT GACTCGTTCA AAGGAGGCAG CAGCGCTCAA 
CACGTTTGTG CCGGGGAGAG CCACTTTGCG GTGGTGACGG TGGAGAAGGA GCTGTTTACT 
TGGGCTAATG TGCAAGGTGG GGCCAAGATG GTGGGCCAGC TGGGGCACGG CGACCAGGCC 
TCTTACCGGC AGCCGAAGAG GGTGGAGAAG CTTCAGGGAA AGGCGATCCG GCAGGTGGCA 
TGTGGGGCCG ACTTCACTGC CTGTGTGACC GACGAAGACC AGCTCTACAT GTTCGGGTCA 
GACTACTACG GCTGCATCGG AGTTGAGGGC GAGATGGGGA TGGAGATTTT GGAGCCTGTT 
CTTCTGGAGT TTTTTGAGGA GCGGTGTGTC TACCAGGTCT CCTGTGGAGA CAACCACGTT 
GTGGTGCTGA CTCAGGATGG CAGCATTTTC TCTTGGGGCT GTGGAGAATA TGGACGTCTT 
GGCCTGGAGT GTGAAGATGA CTTCTCTTCT CCAATGCAAG TGGAAATTCC TAGAGGAGCC 
ACCATCTCCT CTGTGTCGTG TGGCTGTGAT GGGACCTTCT TTCTGACAGA GACTGGGAAA 
GTCTTAGCAT GTGGAAACAA TGAGTACAAC AAGCTGGGGC TGAACCAGGG AATCTCTGGT 
CTCAAAAACC ACCCTGAAAA GGGTTACCAG GGAATACCGT ACATCACCAC GCTGACCTTG 
GTTAAACAGC TGTCGCGCTT CAAGATCCAA GTCATAGCTC CAGGGAAGAC TCACACAGCC 
GCCATTGATG GACGAGGTCG TCTGATCACT TTCGGTTGCA ACAAGTATGG GCAGCTGGGT 
GTGAAGGACT TCAAGAAGCA CCAAGGTGTT CAAGTTCTTG TTGGACCATT CGGAGGGAAG 
ACTGTGACCA AAGTGTCCTG TGGAGATGGA TTCACCATTG CAGCCACTGA GGACAATCAT 
ATCTTTGCAT GGGGAAATGC AGGAAACGGG AGACTTGGAA TGCCCGCAGA TAAAGGATTC 
GGTTCAGAGG TTTGCCCTGC CATGCCGAGG CCCATATTTG GTTCCCTCCA CCACGTGCCT 
GATCTCTCCT GCCGCGGCTG GCACACCATT ATCATAATGG AAAAAGTGCT GAATTCAAAA 
ACCATCCGCT CAAACAGCAG TGGACTATCA ATCGGGCTTG GCCAGGAGGC ATCTACGTCC 
ACGGTGGAGC TGGACATAGA GCCTGGACTG GAGACGGACT GTCGCGACGG CAGTCTTGGG 
GGAACACGGG AGATTAACAC AGAGGAGGGT GTCATGGACA CGTCGATGAT GTCATTCGGA 
AATCAGACGG GGGACAGTTC CTGTCCCCTG TGGCTCAAAA AGGAGCTTGA GGACGCAGAG 
TTCATCCCGA TGCCAGAGGA GTCCGACCAG TCCACTCACG ATCAGCTCTC CTCGATCTCC 
GAGAGCGTCA CTCGGCCATA CGAGGAGCTG AAAGAGCTCA AGGCAGCGGC CGAGGCAGTC 
AGCAGTCAGA AAGGCCTATC GCAAACCAGG CAAATGAGCT TTGAGAAGGT CAGAGGGCTG 
GATGACGACG ACGATGACGT CTTTCCAAAA AAAGAATCCG GTGCGTGCTG CAGTGCAAGT 
ATTGAAGTGG CTCAGCTGAG AGAAACGGTT ACCCGTCAAG AGTCGAGGAT CGAGATGCTT 
GAAAAACAGG TCAGTGAGCA GCAGCGGGAG AACGAAAGGC TCTGGGCAGC GATTAATCGT 
TCAGCGCAGC AGGAAGCAGG ACGTGACGGC AACAGGAATC ATGTCTCTGT GGCGAGCAGT 
GGAGGAGGTG AAAGAAGGCT GACGAACCAC GGAGGCAGAT CTGCTGGAGC AAGTGCCTGA