RBX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RBX1
Entrez Gene ID	9978
Full Name	ring-box 1
Synonyms	BA554C12.1,RNF75,ROC1
General protein information	Preferred Names ring-box 1 Names E3 ubiquitin-protein ligase RBX1 RING finger protein 75 RING-box protein 1 ZYP protein regulator of cullins 1 ring-box 1, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.2
Summary	This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010].
Disorder MIM:	603814

mRNA and Protein(s)

mRNA	Protein	Name
NM_014248.4	NP_055063.1	E3 ubiquitin-protein ligase RBX1
NM_014248.3	NP_055063.1	E3 ubiquitin-protein ligase RBX1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RBX1	NP_055063.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005202	XP_001688524.1
Arabidopsis thaliana (thale cress)	RBX1	NP_001154725.1
Xenopus tropicalis (tropical clawed frog)	rbx1	XP_002934772.2
Pan troglodytes (chimpanzee)	RBX1	XP_515153.2
Canis lupus familiaris (dog)	RBX1	XP_531722.2
Mus musculus (house mouse)	Gm9840	XP_006525295.1
Danio rerio (zebrafish)	rbx1	NP_001077013.1
Bos taurus (cattle)	RBX1	NP_001039706.1
Drosophila melanogaster (fruit fly)	Roc1a	NP_569852.1
Magnaporthe oryzae (rice blast fungus)	MGG_08844	XP_003713864.1
Gallus gallus (chicken)	RBX1	NP_001186179.1
Schizosaccharomyces pombe (fission yeast)	rbx1	NP_593388.1
	LOC710252	XP_001098854.2
Caenorhabditis elegans (roundworm)	rbx-1	NP_505496.1

Gene Ontology
Bibliography

Related articles in PubMed

The Cullin-3-Rbx1-KCTD10 complex controls endothelial barrier function via K63 ubiquitination of RhoB.
Kova?evi? I, Sakaue T, Majole? J, Pronk MC, Maekawa M, Geerts D, Fernandez-Borja M, Higashiyama S, Hordijk PL
The Journal of cell biology(2018 Jan)

RBX1-mediated ubiquitination of SESN2 promotes cell death upon prolonged mitochondrial damage in SH-SY5Y neuroblastoma cells.
Kumar A, Shaha C
Molecular and cellular biochemistry(2018 Jan)

The relation between Ring Box-1 protein overexpression and tumor grade and stage in bladder urothelial cell carcinoma.
Celik ZE, Kaynar M, Karabagli P, Gergerlioglu N, Goktas S
Cancer biomarkers : section A of Disease markers20(4)389-394(2017 Dec)

NOTCH2 Hajdu-Cheney Mutations Escape SCF
Fukushima H, Shimizu K, Watahiki A, Hoshikawa S, Kosho T, Oba D, Sakano S, Arakaki M, Yamada A, Nagashima K, Okabe K, Fukumoto S, Jimi E, Bigas A, Nakayama KI, Nakayama K, Aoki Y, Wei W, Inuzuka H
Molecular cell68(4)645-658.e5(2017 Nov)

The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs.
Garzia A, Jafarnejad SM, Meyer C, Chapat C, Gogakos T, Morozov P, Amiri M, Shapiro M, Molina H, Tuschl T, Sonenberg N
Nature communications816056(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

p97 negatively regulates NRF2 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex.
Title: p97 Negatively Regulates NRF2 by Extracting Ubiquitylated NRF2 from the KEAP1-CUL3 E3 Complex.

Regulation of substrate ubiquitination and polyubiquitination takes place through Rbx1 rotations.
Title: Cullin neddylation may allosterically tune polyubiquitin chain length and topology.

High RBX1 expression was related to poor tumor differentiation, advanced TNM stage, and lymph node metastasis in non-small cell lung cancer.
Title: RBX1 expression is an unfavorable prognostic factor in patients with non-small cell lung cancer.

ROC1 has an important role in the malignant progression of bladder transitional cell carcinoma via the mTOR/DEPTOR pathway.
Title: Regulator of cullins-1 expression knockdown suppresses the malignant progression of muscle-invasive transitional cell carcinoma by regulating mTOR/DEPTOR pathway.

Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8.
Title: Gln40 deamidation blocks structural reconfiguration and activation of SCF ubiquitin ligase complex by Nedd8.

The following RBX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55616	NM_014248.4 Latest version!	Homo sapiens ring-box 1 (RBX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55616	NM_014248.3	Homo sapiens ring-box 1 (RBX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RBX1 ( NM_014248.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055063.1 Homo sapiens ring-box 1 (RBX1), mRNA.

Price & Availability↑

CloneID	OHu55616
Clone ID Related Accession (Same CDS sequence)	NM_014248.4 , NM_014248.3
Accession Version	NM_014248.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RBX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA353856.1, AF140598.1, DN602016.1 and AL080242.11. On May 17, 2019 this sequence version replaced NM_014248.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.60101.1, SRR1803614.52727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216225.9/ ENSP00000216225.8 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGATGGATGT GGATACCCCG AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TGAAAAAGTG GAATGCAGTA GCCCTCTGGG CCTGGGATAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGCA TAGAATGTCA AGCTAACCAG 
GCGTCCGCTA CTTCAGAAGA GTGTACTGTC GCATGGGGAG TCTGTAACCA TGCTTTTCAC 
TTCCACTGCA TCTCTCGCTG GCTCAAAACA CGACAGGTGT GTCCATTGGA CAACAGAGAG 
TGGGAATTCC AAAAGTATGG GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055063.1
CDS	22..348
Translation	MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Target ORF information:

RefSeq Version	NM_014248.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring-box 1 (RBX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014248.4

ORF Insert Sequence:

ATGGCGGCAG CGATGGATGT GGATACCCCG AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TGAAAAAGTG GAATGCAGTA GCCCTCTGGG CCTGGGATAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGCA TAGAATGTCA AGCTAACCAG 
GCGTCCGCTA CTTCAGAAGA GTGTACTGTC GCATGGGGAG TCTGTAACCA TGCTTTTCAC 
TTCCACTGCA TCTCTCGCTG GCTCAAAACA CGACAGGTGT GTCCATTGGA CAACAGAGAG 
TGGGAATTCC AAAAGTATGG GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBX1 ( NM_014248.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055063.1 Homo sapiens ring-box 1 (RBX1), mRNA.

Price & Availability↑

CloneID	OHu55616
Clone ID Related Accession (Same CDS sequence)	NM_014248.4 , NM_014248.3
Accession Version	NM_014248.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RBX1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA353856.1, AF140598.1 and DN602016.1. On Sep 21, 2010 this sequence version replaced NM_014248.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.136464.1, SRR1660809.269295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAG CGATGGATGT GGATACCCCG AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TGAAAAAGTG GAATGCAGTA GCCCTCTGGG CCTGGGATAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGCA TAGAATGTCA AGCTAACCAG 
GCGTCCGCTA CTTCAGAAGA GTGTACTGTC GCATGGGGAG TCTGTAACCA TGCTTTTCAC 
TTCCACTGCA TCTCTCGCTG GCTCAAAACA CGACAGGTGT GTCCATTGGA CAACAGAGAG 
TGGGAATTCC AAAAGTATGG GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055063.1
CDS	53..379
Translation	MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

Target ORF information:

RefSeq Version	NM_014248.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring-box 1 (RBX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014248.3

ORF Insert Sequence:

ATGGCGGCAG CGATGGATGT GGATACCCCG AGCGGCACCA ACAGCGGCGC GGGCAAGAAG 
CGCTTTGAAG TGAAAAAGTG GAATGCAGTA GCCCTCTGGG CCTGGGATAT TGTGGTTGAT 
AACTGTGCCA TCTGCAGGAA CCACATTATG GATCTTTGCA TAGAATGTCA AGCTAACCAG 
GCGTCCGCTA CTTCAGAAGA GTGTACTGTC GCATGGGGAG TCTGTAACCA TGCTTTTCAC 
TTCCACTGCA TCTCTCGCTG GCTCAAAACA CGACAGGTGT GTCCATTGGA CAACAGAGAG 
TGGGAATTCC AAAAGTATGG GCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Cullin-3-Rbx1-KCTD10 complex controls endothelial barrier function via K63 ubiquitination of RhoB.
The Journal of cell biology(2018 Jan)
Kova?evi? I,Sakaue T,Majole? J,Pronk MC,Maekawa M,Geerts D,Fernandez-Borja M,Higashiyama S,Hordijk PL

RBX1-mediated ubiquitination of SESN2 promotes cell death upon prolonged mitochondrial damage in SH-SY5Y neuroblastoma cells.
Molecular and cellular biochemistry(2018 Jan)
Kumar A,Shaha C

The relation between Ring Box-1 protein overexpression and tumor grade and stage in bladder urothelial cell carcinoma.
Cancer biomarkers : section A of Disease markers20(4)389-394(2017 Dec)
Celik ZE,Kaynar M,Karabagli P,Gergerlioglu N,Goktas S

NOTCH2 Hajdu-Cheney Mutations Escape SCF
Molecular cell68(4)645-658.e5(2017 Nov)
Fukushima H,Shimizu K,Watahiki A,Hoshikawa S,Kosho T,Oba D,Sakano S,Arakaki M,Yamada A,Nagashima K,Okabe K,Fukumoto S,Jimi E,Bigas A,Nakayama KI,Nakayama K,Aoki Y,Wei W,Inuzuka H

The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs.
Nature communications816056(2017 Jul)
Garzia A,Jafarnejad SM,Meyer C,Chapat C,Gogakos T,Morozov P,Amiri M,Shapiro M,Molina H,Tuschl T,Sonenberg N

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RBX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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