WASHC5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WASHC5
Entrez Gene ID	9897
Full Name	WASH complex subunit 5
Synonyms	KIAA0196,RTSC,RTSC1,SPG8
General protein information	Preferred Names WASH complex subunit 5 Names WASH complex subunit 5 WASH complex subunit strumpellin strumpellin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.13
Summary	This gene encodes a 134 kDa protein named strumpellin that is predicted to have multiple transmembrane domains and a spectrin-repeat-containing domain. This ubiquitously expressed gene has its highest expression in skeletal muscle. The protein is named for Strumpell disease; a form of hereditary spastic paraplegia (HSP). Spastic paraplegias are a diverse group of disorders in which the autosomal dominant forms are characterized by progressive, lower extremity spasticity caused by axonal degeneration in the terminal portions of the longest descending and ascending corticospinal tracts. More than 30 loci (SPG1-33) have been implicated in hereditary spastic paraplegia diseases. [provided by RefSeq, Aug 2009].
Disorder MIM:	610657
Disorder Html:	Spastic paraplegia 8, autosomal dominant, 603563 (3); Ritscher-Schinzel syndrome 1, 220210 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014846.4	NP_055661.3	WASH complex subunit 5 isoform 1
NM_001330609.1	NP_001317538.1	WASH complex subunit 5 isoform 2
XM_017014113.1	XP_016869602.1	WASH complex subunit strumpellin isoform X1
XM_017014113.2	XP_016869602.1	WASH complex subunit 5 isoform X1
NM_014846.3	NP_055661.3	WASH complex subunit 5 isoform 1
XM_011517409.1	XP_011515711.1	WASH complex subunit 5 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KIAA0196	XP_418441.2
Mus musculus (house mouse)	E430025E21Rik	NP_705776.2
Rattus norvegicus (Norway rat)	RGD1564420	XP_002729862.2
Xenopus tropicalis (tropical clawed frog)	kiaa0196	NP_001004970.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007019	XP_308754.3
Caenorhabditis elegans (roundworm)	T05E7.3	NP_491784.1
Oryza sativa (rice)	Os04g0398800	NP_001052675.1
Bos taurus (cattle)	KIAA0196	XP_002692668.1
Homo sapiens (human)	KIAA0196	NP_055661.3
Pan troglodytes (chimpanzee)	KIAA0196	XP_519952.2
	LOC706857	XP_002805529.1
Canis lupus familiaris (dog)	KIAA0196	XP_532327.2
Danio rerio (zebrafish)	strumpellin	NP_956477.1
Drosophila melanogaster (fruit fly)	CG12272	NP_001163454.1

Gene Ontology
Bibliography

Related articles in PubMed

Exome sequencing reveals a novel missense mutation in the KIAA0196 gene in a Japanese patient with SPG8.
Ichinose Y, Koh K, Fukumoto M, Yamashiro N, Kobayashi F, Miwa M, Nagasaka T, Shindo K, Ishiura H, Tsuji S, Takiyama DY
Clinical neurology and neurosurgery14436-8(2016 May)

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
Bartuzi P, Billadeau DD, Favier R, Rong S, Dekker D, Fedoseienko A, Fieten H, Wijers M, Levels JH, Huijkman N, Kloosterhuis N, van der Molen H, Brufau G, Groen AK, Elliott AM, Kuivenhoven JA, Plecko B, Grangl G, McGaughran J, Horton JD, Burstein E, Hofker MH, van de Sluis B
Nature communications710961(2016 Mar)

C9orf72 and UNC13A are shared risk loci for amyotrophic lateral sclerosis and frontotemporal dementia: a genome-wide meta-analysis.
Diekstra FP, Van Deerlin VM, van Swieten JC, Al-Chalabi A, Ludolph AC, Weishaupt JH, Hardiman O, Landers JE, Brown RH Jr, van Es MA, Pasterkamp RJ, Koppers M, Andersen PM, Estrada K, Rivadeneira F, Hofman A, Uitterlinden AG, van Damme P, Melki J, Meininger V, Shatunov A, Shaw CE, Leigh PN, Shaw PJ, Morrison KE, Fogh I, Chi? A, Traynor BJ, Czell D, Weber M, Heutink P, de Bakker PI, Silani V, Robberecht W, van den Berg LH, Veldink JH
Annals of neurology76(1)120-33(2014 Jul)

A novel KIAA0196 (SPG8) mutation in a Chinese family with spastic paraplegia.
Wang X, Yang Y, Wang X, Li C, Jia J
Chinese medical journal127(10)1987-9(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel missense mutation was identified in the KIAA0196 gene in a Japanese patient with SPG8.
Title: Exome sequencing reveals a novel missense mutation in the KIAA0196 gene in a Japanese patient with SPG8.

A mutation in the WASH component KIAA0196 (strumpellin) is associated with hypercholesterolaemia in humans.
Title: CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.

Study found a novel KIAA0196 (SPG8) mutation in a Chinese family with spastic paraplegia.
Title: A novel KIAA0196 (SPG8) mutation in a Chinese family with spastic paraplegia.

To identify the underlying genetic cause of Ritscher-Schinzel syndrome, affected individuals from a First Nations community in Manitoba underwent mutational analysis. All eight patients were homozygous for a novel splice site mutation in KIAA0196.
Title: A novel mutation in KIAA0196: identification of a gene involved in Ritscher-Schinzel/3C syndrome in a First Nations cohort.

we identified a novel KIAA0196 missense variant in the proband and her daughter expanding the clinical spectrum of hereditary spastic paraplegia 8.
Title: Exome sequencing expands the mutational spectrum of SPG8 in a family with spasticity responsive to L-DOPA treatment.

The following WASHC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WASHC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18519	NM_014846.4 Latest version!	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu18519	XM_017014113.2 Latest version!	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu42641	NM_001330609.1 Latest version!	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu63472	XM_011517409.1 Latest version!	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu18519	XM_017014113.1	Homo sapiens KIAA0196 (KIAA0196), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu18519	NM_014846.3	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WASHC5 ( NM_014846.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055661.3 Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18519
Clone ID Related Accession (Same CDS sequence)	NM_014846.4 , XM_017014113.1 , XM_017014113.2 , NM_014846.3
Accession Version	NM_014846.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	WASH complex subunit 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA185885.1, D83780.1, BC106015.1 and AI266595.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014846.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.160216.1, SRR1803612.28663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000318410.12/ ENSP00000318016.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055661.3
CDS	303..3782
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	NM_014846.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014846.4

ORF Insert Sequence:

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC5 ( NM_014846.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055661.3 Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18519
Clone ID Related Accession (Same CDS sequence)	NM_014846.4 , XM_017014113.1 , XM_017014113.2 , NM_014846.3
Accession Version	NM_014846.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	WASH complex subunit 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA185885.1, D83780.1, BC106015.1 and AI266595.1. This sequence is a reference standard in the RefSeqGene project. On Jan 5, 2007 this sequence version replaced NM_014846.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.17342.1, SRR1660809.105287.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055661.3
CDS	330..3809
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	NM_014846.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014846.3

ORF Insert Sequence:

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC5 ( XM_017014113.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869602.1 Homo sapiens KIAA0196 (KIAA0196), transcript variant X1, mRNA.

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CloneID	OHu18519
Clone ID Related Accession (Same CDS sequence)	NM_014846.4 , XM_017014113.1 , XM_017014113.2 , NM_014846.3
Accession Version	XM_017014113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	WASH complex subunit strumpellin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869602.1
CDS	535..4014
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	XM_017014113.1
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA0196 (KIAA0196), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014113.1

ORF Insert Sequence:

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC5 ( XM_017014113.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869602.1 Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X1, mRNA.

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CloneID	OHu18519
Clone ID Related Accession (Same CDS sequence)	NM_014846.4 , XM_017014113.1 , XM_017014113.2 , NM_014846.3
Accession Version	XM_017014113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	WASH complex subunit 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869602.1
CDS	536..4015
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	XM_017014113.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014113.2

ORF Insert Sequence:

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGAATGGTCT GCCGAAAACC GACCGACCCG GTTGATTGGC CACCACTTGT CCTGGGACTG 
CTCACTCTGC TGAAGCAGTT CCATTCCCGG TACACCGAGC AGTTCCTGGC GCTGATTGGC 
CAGTTTATCT GCTCCACGGT GGAGCAGTGT ACAAGCCAGA AGATACCTGA AATTCCTGCA 
GATGTTGTGG GTGCCCTTCT GTTCCTGGAG GATTATGTTC GGTACACAAA GCTACCCAGG 
AGGGTTGCTG AAGCACATGT GCCTAATTTC ATTTTTGATG AGTTCAGAAC AGTGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC5 ( NM_001330609.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317538.1 Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 2, mRNA.

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CloneID	OHu42641
Clone ID Related Accession (Same CDS sequence)	NM_001330609.1
Accession Version	NM_001330609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-20
Organism	Homo sapiens(human)
Product	WASH complex subunit 5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA189452.1, CX165397.1, AL134860.1, AC009908.13, CX781999.1, CN307656.1 and AA810712.1. On Aug 30, 2016 this sequence version replaced XM_005251120.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.15362.1, SRR1803613.151696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCTACTGG TCATTGACCA AAAGATTGAA GGAGAAGTCA GAGAGAGGAT GCTGGTTTCT 
TACTACCGAT ACAGTGCTGC TCGATCTTCT GCTGATTCAA ATATGGACGA TATTTGTAAG 
CTGCTTCGAA GTACAGGTTA TTCTAGCCAA CCAGGTGCCA AAAGACCATC CAACTATCCC 
GAGAGCTATT TCCAGAGAGT GCCTATCAAC GAATCCTTCA TCAGTATGGT CATTGGTCGA 
CTGAGATCTG ATGATATTTA CAACCAGGTC TCAGCGTATC CTTTGCCGGA GCATCGCAGC 
ACAGCCCTGG CAAACCAAGC TGCCATGCTG TACGTGATTC TCTACTTTGA GCCTTCCATC 
CTTCACACCC ATCAAGCAAA AATGAGAGAG ATAGTGGATA AATACTTTCC AGATAATTGG 
GTAATTAGTA TTTACATGGG GATCACAGTT AATCTAGTAG ATGCTTGGGA ACCTTACAAA 
GCTGCAAAAA CTGCTTTAAA TAATACCCTG GACCTTTCAA ATGTCAGAGA ACAGGCAAGC 
AGATATGCTA CTGTCAGTGA AAGAGTGCAT GCTCAAGTGC AGCAATTTCT AAAAGAAGGT 
TATTTAAGGG AGGAGATGGT TCTGGACAAT ATCCCAAAGC TTCTGAACTG CCTGAGAGAC 
TGCAATGTTG CCATCCGATG GCTGATGCTT CATACAGCAG ACTCAGCCTG TGACCCAAAC 
AACAAACGCC TTCGTCAAAT CAAGGACCAG ATTCTAACAG ACTCTCGGTA CAATCCCAGG 
ATCCTCTTCC AGCTGCTGTT AGATACTGCA CAATTTGAGT TTATACTCAA AGAGATGTTC 
AAGCAAATGC TTTCAGAAAA GCAAACCAAA TGGGAGCATT ACAAGAAAGA GGGTTCGGAG 
CGGATGACTG AGCTTGCTGA TGTCTTTTCA GGAGTGAAAC CCCTAACCAG AGTGGAGAAA 
AATGAAAACC TTCAAGCTTG GTTCAGAGAG ATCTCAAAAC AAATATTGTC TTTAAATTAT 
GATGATTCTA CTGCTGCGGG CAGAAAAACT GTACAACTGA TACAAGCTTT GGAAGAGGTT 
CAAGAATTCC ACCAGTTGGA ATCCAATCTG CAAGTATGTC AGTTTCTTGC CGATACTCGA 
AAGTTTCTTC ATCAAATGAT CAGAACCATT AACATTAAAG AGGAGGTTCT GATCACAATG 
CAGATCGTTG GGGACCTTTC TTTCGCTTGG CAGTTGATTG ACAGTTTCAC ATCCATCATG 
CAAGAAAGCA TAAGGGTAAA TCCATCCATG GTTACTAAAC TCAGAGCTAC CTTCCTAAAG 
CTTGCCTCTG CCCTCGATCT GCCCCTTCTT CGTATTAATC AGGCAAATAG CCCCGACCTG 
CTCAGCGTGT CACAGTACTA TTCTGGAGAG TTGGTATCCT ATGTGAGAAA AGTTTTGCAG 
ATCATCCCAG AAAGCATGTT TACATCTCTT CTAAAGATCA TAAAGCTTCA GACCCACGAC 
ATTATTGAAG TGCCTACCCG CCTGGACAAA GACAAGCTGA GGGACTATGC TCAGCTAGGC 
CCACGATACG AGGTTGCCAA GCTTACTCAT GCTATTTCCA TTTTTACTGA AGGCATCTTA 
ATGATGAAAA CGACTTTGGT TGGCATCATC AAGGTGGATC CAAAGCAGTT GCTGGAAGAT 
GGAATAAGGA AAGAGCTTGT GAAGCGCGTT GCCTTTGCCC TGCATAGGGG ACTGATATTC 
AACCCTCGAG CCAAGCCAAG TGAATTGATG CCCAAGCTGA AAGAGTTGGG AGCGACCATG 
GATGGATTCC ATCGTTCTTT TGAATACATA CAGGACTATG TCAACATTTA TGGTCTGAAG 
ATTTGGCAGG AAGAAGTATC TCGTATCATA AATTACAACG TGGAGCAAGA GTGTAATAAC 
TTTCTAAGAA CGAAGATTCA AGATTGGCAA AGCATGTACC AGTCCACTCA TATTCCAATA 
CCCAAGTTTA CCCCTGTGGA TGAGTCTGTA ACGTTTATTG GTCGACTCTG CAGAGAAATC 
CTGCGGATCA CAGACCCAAA AATGACATGT CACATAGACC AGCTGAACAC TTGGTATGAT 
ATGAAAACTC ATCAGGAAGT GACCAGCAGC CGCCTCTTCT CAGAAATCCA GACCACCTTG 
GGAACCTTTG GTCTAAATGG CTTAGACAGG CTTCTGTGCT TTATGATTGT AAAAGAGTTA 
CAGAATTTCC TCAGTATGTT TCAGAAAATT ATCCTGAGAG ACAGAACTGT TCAGGACACT 
TTAAAAACCC TCATGAATGC TGTCAGTCCC CTAAAAAGTA TTGTCGCAAA TTCAAATAAA 
ATTTATTTTT CCGCCATTGC CAAAACACAG AAGATTTGGA CTGCGTATCT CGAGGCTATA 
ATGAAGGTTG GGCAGATGCA GATTCTGAGA CAACAGATTG CCAATGAATT AAATTATTCT 
TGTCGGTTTG ATTCTAAACA TCTGGCAGCT GCTCTGGAGA ATCTCAATAA GGCTCTCCTA 
GCAGACATTG AAGCCCACTA TCAGGACCCT TCACTTCCTT ACCCCAAAGA AGATAACACA 
CTTTTATATG AAATCACAGC CTATCTGGAG GCAGCTGGCA TTCACAACCC ACTGAATAAG 
ATATACATAA CAACAAAGCG CTTACCCTAT TTTCCAATTG TAAACTTTCT ATTTTTGATC 
GCTCAGTTGC CAAAACTTCA ATACAACAAA AATCTGGGAA TGGTCTGCCG AAAACCGACC 
GACCCGGTTG ATTGGCCACC ACTTGTCCTG GGACTGCTCA CTCTGCTGAA GCAGTTCCAT 
TCCCGGTACA CCGAGCAGTT CCTGGCGCTG ATTGGCCAGT TTATCTGCTC CACGGTGGAG 
CAGTGTACAA GCCAGAAGAT ACCTGAAATT CCTGCAGATG TTGTGGGTGC CCTTCTGTTC 
CTGGAGGATT ATGTTCGGTA CACAAAGCTA CCCAGGAGGG TTGCTGAAGC ACATGTGCCT 
AATTTCATTT TTGATGAGTT CAGAACAGTG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317538.1
CDS	464..3499
Translation	MLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPADVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL

Target ORF information:

RefSeq Version	NM_001330609.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330609.1

ORF Insert Sequence:

ATGCTACTGG TCATTGACCA AAAGATTGAA GGAGAAGTCA GAGAGAGGAT GCTGGTTTCT 
TACTACCGAT ACAGTGCTGC TCGATCTTCT GCTGATTCAA ATATGGACGA TATTTGTAAG 
CTGCTTCGAA GTACAGGTTA TTCTAGCCAA CCAGGTGCCA AAAGACCATC CAACTATCCC 
GAGAGCTATT TCCAGAGAGT GCCTATCAAC GAATCCTTCA TCAGTATGGT CATTGGTCGA 
CTGAGATCTG ATGATATTTA CAACCAGGTC TCAGCGTATC CTTTGCCGGA GCATCGCAGC 
ACAGCCCTGG CAAACCAAGC TGCCATGCTG TACGTGATTC TCTACTTTGA GCCTTCCATC 
CTTCACACCC ATCAAGCAAA AATGAGAGAG ATAGTGGATA AATACTTTCC AGATAATTGG 
GTAATTAGTA TTTACATGGG GATCACAGTT AATCTAGTAG ATGCTTGGGA ACCTTACAAA 
GCTGCAAAAA CTGCTTTAAA TAATACCCTG GACCTTTCAA ATGTCAGAGA ACAGGCAAGC 
AGATATGCTA CTGTCAGTGA AAGAGTGCAT GCTCAAGTGC AGCAATTTCT AAAAGAAGGT 
TATTTAAGGG AGGAGATGGT TCTGGACAAT ATCCCAAAGC TTCTGAACTG CCTGAGAGAC 
TGCAATGTTG CCATCCGATG GCTGATGCTT CATACAGCAG ACTCAGCCTG TGACCCAAAC 
AACAAACGCC TTCGTCAAAT CAAGGACCAG ATTCTAACAG ACTCTCGGTA CAATCCCAGG 
ATCCTCTTCC AGCTGCTGTT AGATACTGCA CAATTTGAGT TTATACTCAA AGAGATGTTC 
AAGCAAATGC TTTCAGAAAA GCAAACCAAA TGGGAGCATT ACAAGAAAGA GGGTTCGGAG 
CGGATGACTG AGCTTGCTGA TGTCTTTTCA GGAGTGAAAC CCCTAACCAG AGTGGAGAAA 
AATGAAAACC TTCAAGCTTG GTTCAGAGAG ATCTCAAAAC AAATATTGTC TTTAAATTAT 
GATGATTCTA CTGCTGCGGG CAGAAAAACT GTACAACTGA TACAAGCTTT GGAAGAGGTT 
CAAGAATTCC ACCAGTTGGA ATCCAATCTG CAAGTATGTC AGTTTCTTGC CGATACTCGA 
AAGTTTCTTC ATCAAATGAT CAGAACCATT AACATTAAAG AGGAGGTTCT GATCACAATG 
CAGATCGTTG GGGACCTTTC TTTCGCTTGG CAGTTGATTG ACAGTTTCAC ATCCATCATG 
CAAGAAAGCA TAAGGGTAAA TCCATCCATG GTTACTAAAC TCAGAGCTAC CTTCCTAAAG 
CTTGCCTCTG CCCTCGATCT GCCCCTTCTT CGTATTAATC AGGCAAATAG CCCCGACCTG 
CTCAGCGTGT CACAGTACTA TTCTGGAGAG TTGGTATCCT ATGTGAGAAA AGTTTTGCAG 
ATCATCCCAG AAAGCATGTT TACATCTCTT CTAAAGATCA TAAAGCTTCA GACCCACGAC 
ATTATTGAAG TGCCTACCCG CCTGGACAAA GACAAGCTGA GGGACTATGC TCAGCTAGGC 
CCACGATACG AGGTTGCCAA GCTTACTCAT GCTATTTCCA TTTTTACTGA AGGCATCTTA 
ATGATGAAAA CGACTTTGGT TGGCATCATC AAGGTGGATC CAAAGCAGTT GCTGGAAGAT 
GGAATAAGGA AAGAGCTTGT GAAGCGCGTT GCCTTTGCCC TGCATAGGGG ACTGATATTC 
AACCCTCGAG CCAAGCCAAG TGAATTGATG CCCAAGCTGA AAGAGTTGGG AGCGACCATG 
GATGGATTCC ATCGTTCTTT TGAATACATA CAGGACTATG TCAACATTTA TGGTCTGAAG 
ATTTGGCAGG AAGAAGTATC TCGTATCATA AATTACAACG TGGAGCAAGA GTGTAATAAC 
TTTCTAAGAA CGAAGATTCA AGATTGGCAA AGCATGTACC AGTCCACTCA TATTCCAATA 
CCCAAGTTTA CCCCTGTGGA TGAGTCTGTA ACGTTTATTG GTCGACTCTG CAGAGAAATC 
CTGCGGATCA CAGACCCAAA AATGACATGT CACATAGACC AGCTGAACAC TTGGTATGAT 
ATGAAAACTC ATCAGGAAGT GACCAGCAGC CGCCTCTTCT CAGAAATCCA GACCACCTTG 
GGAACCTTTG GTCTAAATGG CTTAGACAGG CTTCTGTGCT TTATGATTGT AAAAGAGTTA 
CAGAATTTCC TCAGTATGTT TCAGAAAATT ATCCTGAGAG ACAGAACTGT TCAGGACACT 
TTAAAAACCC TCATGAATGC TGTCAGTCCC CTAAAAAGTA TTGTCGCAAA TTCAAATAAA 
ATTTATTTTT CCGCCATTGC CAAAACACAG AAGATTTGGA CTGCGTATCT CGAGGCTATA 
ATGAAGGTTG GGCAGATGCA GATTCTGAGA CAACAGATTG CCAATGAATT AAATTATTCT 
TGTCGGTTTG ATTCTAAACA TCTGGCAGCT GCTCTGGAGA ATCTCAATAA GGCTCTCCTA 
GCAGACATTG AAGCCCACTA TCAGGACCCT TCACTTCCTT ACCCCAAAGA AGATAACACA 
CTTTTATATG AAATCACAGC CTATCTGGAG GCAGCTGGCA TTCACAACCC ACTGAATAAG 
ATATACATAA CAACAAAGCG CTTACCCTAT TTTCCAATTG TAAACTTTCT ATTTTTGATC 
GCTCAGTTGC CAAAACTTCA ATACAACAAA AATCTGGGAA TGGTCTGCCG AAAACCGACC 
GACCCGGTTG ATTGGCCACC ACTTGTCCTG GGACTGCTCA CTCTGCTGAA GCAGTTCCAT 
TCCCGGTACA CCGAGCAGTT CCTGGCGCTG ATTGGCCAGT TTATCTGCTC CACGGTGGAG 
CAGTGTACAA GCCAGAAGAT ACCTGAAATT CCTGCAGATG TTGTGGGTGC CCTTCTGTTC 
CTGGAGGATT ATGTTCGGTA CACAAAGCTA CCCAGGAGGG TTGCTGAAGC ACATGTGCCT 
AATTTCATTT TTGATGAGTT CAGAACAGTG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC5 ( XM_011517409.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011515711.1 Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X2, mRNA.

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CloneID	OHu63472
Clone ID Related Accession (Same CDS sequence)	XM_011517409.1
Accession Version	XM_011517409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	WASH complex subunit 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGATGCATGT GCCAGCTGTG TATCTGGAAC CATGATGGGA AAAGTAACAA TATAGTTAAG 
GAGATAAGAC TAATATTTAT ACATGAACAT TTAATACTTT ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011515711.1
CDS	307..3594
Translation	MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESKLDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPESYFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIVDKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYLREEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQFEFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHIDQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLKTLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAALENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGCMCQLCIWNHDGKSNNIVKEIRLIFIHEHLILYV

Target ORF information:

RefSeq Version	XM_011517409.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WASH complex subunit 5 (WASHC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517409.1

ORF Insert Sequence:

ATGTTGGACT TTCTAGCCGA GAACAACCTC TGTGGCCAAG CAATCCTAAG GATTGTTTCC 
TGTGGTAATG CCATCATTGC TGAACTTTTG AGACTCTCTG AGTTTATTCC TGCTGTGTTC 
AGGTTAAAAG ACAGAGCTGA TCAACAGAAA TATGGAGATA TCATATTTGA TTTCAGCTAT 
TTTAAGGGTC CAGAATTATG GGAAAGCAAA CTGGATGCTA AGCCAGAGCT ACAGGATTTA 
GATGAAGAAT TTCGTGAAAA CAACATAGAA ATTGTGACCA GATTTTATTT AGCATTTCAA 
AGTGTACATA AATATATTGT AGACTTAAAC AGATATCTAG ATGATCTCAA TGAAGGGGTT 
TATATTCAGC AAACCTTAGA AACTGTGCTT CTCAATGAAG ATGGAAAACA ACTTCTATGT 
GAAGCACTGT ACTTATATGG AGTTATGCTA CTGGTCATTG ACCAAAAGAT TGAAGGAGAA 
GTCAGAGAGA GGATGCTGGT TTCTTACTAC CGATACAGTG CTGCTCGATC TTCTGCTGAT 
TCAAATATGG ACGATATTTG TAAGCTGCTT CGAAGTACAG GTTATTCTAG CCAACCAGGT 
GCCAAAAGAC CATCCAACTA TCCCGAGAGC TATTTCCAGA GAGTGCCTAT CAACGAATCC 
TTCATCAGTA TGGTCATTGG TCGACTGAGA TCTGATGATA TTTACAACCA GGTCTCAGCG 
TATCCTTTGC CGGAGCATCG CAGCACAGCC CTGGCAAACC AAGCTGCCAT GCTGTACGTG 
ATTCTCTACT TTGAGCCTTC CATCCTTCAC ACCCATCAAG CAAAAATGAG AGAGATAGTG 
GATAAATACT TTCCAGATAA TTGGGTAATT AGTATTTACA TGGGGATCAC AGTTAATCTA 
GTAGATGCTT GGGAACCTTA CAAAGCTGCA AAAACTGCTT TAAATAATAC CCTGGACCTT 
TCAAATGTCA GAGAACAGGC AAGCAGATAT GCTACTGTCA GTGAAAGAGT GCATGCTCAA 
GTGCAGCAAT TTCTAAAAGA AGGTTATTTA AGGGAGGAGA TGGTTCTGGA CAATATCCCA 
AAGCTTCTGA ACTGCCTGAG AGACTGCAAT GTTGCCATCC GATGGCTGAT GCTTCATACA 
GCAGACTCAG CCTGTGACCC AAACAACAAA CGCCTTCGTC AAATCAAGGA CCAGATTCTA 
ACAGACTCTC GGTACAATCC CAGGATCCTC TTCCAGCTGC TGTTAGATAC TGCACAATTT 
GAGTTTATAC TCAAAGAGAT GTTCAAGCAA ATGCTTTCAG AAAAGCAAAC CAAATGGGAG 
CATTACAAGA AAGAGGGTTC GGAGCGGATG ACTGAGCTTG CTGATGTCTT TTCAGGAGTG 
AAACCCCTAA CCAGAGTGGA GAAAAATGAA AACCTTCAAG CTTGGTTCAG AGAGATCTCA 
AAACAAATAT TGTCTTTAAA TTATGATGAT TCTACTGCTG CGGGCAGAAA AACTGTACAA 
CTGATACAAG CTTTGGAAGA GGTTCAAGAA TTCCACCAGT TGGAATCCAA TCTGCAAGTA 
TGTCAGTTTC TTGCCGATAC TCGAAAGTTT CTTCATCAAA TGATCAGAAC CATTAACATT 
AAAGAGGAGG TTCTGATCAC AATGCAGATC GTTGGGGACC TTTCTTTCGC TTGGCAGTTG 
ATTGACAGTT TCACATCCAT CATGCAAGAA AGCATAAGGG TAAATCCATC CATGGTTACT 
AAACTCAGAG CTACCTTCCT AAAGCTTGCC TCTGCCCTCG ATCTGCCCCT TCTTCGTATT 
AATCAGGCAA ATAGCCCCGA CCTGCTCAGC GTGTCACAGT ACTATTCTGG AGAGTTGGTA 
TCCTATGTGA GAAAAGTTTT GCAGATCATC CCAGAAAGCA TGTTTACATC TCTTCTAAAG 
ATCATAAAGC TTCAGACCCA CGACATTATT GAAGTGCCTA CCCGCCTGGA CAAAGACAAG 
CTGAGGGACT ATGCTCAGCT AGGCCCACGA TACGAGGTTG CCAAGCTTAC TCATGCTATT 
TCCATTTTTA CTGAAGGCAT CTTAATGATG AAAACGACTT TGGTTGGCAT CATCAAGGTG 
GATCCAAAGC AGTTGCTGGA AGATGGAATA AGGAAAGAGC TTGTGAAGCG CGTTGCCTTT 
GCCCTGCATA GGGGACTGAT ATTCAACCCT CGAGCCAAGC CAAGTGAATT GATGCCCAAG 
CTGAAAGAGT TGGGAGCGAC CATGGATGGA TTCCATCGTT CTTTTGAATA CATACAGGAC 
TATGTCAACA TTTATGGTCT GAAGATTTGG CAGGAAGAAG TATCTCGTAT CATAAATTAC 
AACGTGGAGC AAGAGTGTAA TAACTTTCTA AGAACGAAGA TTCAAGATTG GCAAAGCATG 
TACCAGTCCA CTCATATTCC AATACCCAAG TTTACCCCTG TGGATGAGTC TGTAACGTTT 
ATTGGTCGAC TCTGCAGAGA AATCCTGCGG ATCACAGACC CAAAAATGAC ATGTCACATA 
GACCAGCTGA ACACTTGGTA TGATATGAAA ACTCATCAGG AAGTGACCAG CAGCCGCCTC 
TTCTCAGAAA TCCAGACCAC CTTGGGAACC TTTGGTCTAA ATGGCTTAGA CAGGCTTCTG 
TGCTTTATGA TTGTAAAAGA GTTACAGAAT TTCCTCAGTA TGTTTCAGAA AATTATCCTG 
AGAGACAGAA CTGTTCAGGA CACTTTAAAA ACCCTCATGA ATGCTGTCAG TCCCCTAAAA 
AGTATTGTCG CAAATTCAAA TAAAATTTAT TTTTCCGCCA TTGCCAAAAC ACAGAAGATT 
TGGACTGCGT ATCTCGAGGC TATAATGAAG GTTGGGCAGA TGCAGATTCT GAGACAACAG 
ATTGCCAATG AATTAAATTA TTCTTGTCGG TTTGATTCTA AACATCTGGC AGCTGCTCTG 
GAGAATCTCA ATAAGGCTCT CCTAGCAGAC ATTGAAGCCC ACTATCAGGA CCCTTCACTT 
CCTTACCCCA AAGAAGATAA CACACTTTTA TATGAAATCA CAGCCTATCT GGAGGCAGCT 
GGCATTCACA ACCCACTGAA TAAGATATAC ATAACAACAA AGCGCTTACC CTATTTTCCA 
ATTGTAAACT TTCTATTTTT GATCGCTCAG TTGCCAAAAC TTCAATACAA CAAAAATCTG 
GGATGCATGT GCCAGCTGTG TATCTGGAAC CATGATGGGA AAAGTAACAA TATAGTTAAG 
GAGATAAGAC TAATATTTAT ACATGAACAT TTAATACTTT ATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome sequencing reveals a novel missense mutation in the KIAA0196 gene in a Japanese patient with SPG8.
Clinical neurology and neurosurgery14436-8(2016 May)
Ichinose Y,Koh K,Fukumoto M,Yamashiro N,Kobayashi F,Miwa M,Nagasaka T,Shindo K,Ishiura H,Tsuji S,Takiyama DY

CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
Nature communications710961(2016 Mar)
Bartuzi P,Billadeau DD,Favier R,Rong S,Dekker D,Fedoseienko A,Fieten H,Wijers M,Levels JH,Huijkman N,Kloosterhuis N,van der Molen H,Brufau G,Groen AK,Elliott AM,Kuivenhoven JA,Plecko B,Grangl G,McGaughran J,Horton JD,Burstein E,Hofker MH,van de Sluis B

C9orf72 and UNC13A are shared risk loci for amyotrophic lateral sclerosis and frontotemporal dementia: a genome-wide meta-analysis.
Annals of neurology76(1)120-33(2014 Jul)
Diekstra FP,Van Deerlin VM,van Swieten JC,Al-Chalabi A,Ludolph AC,Weishaupt JH,Hardiman O,Landers JE,Brown RH Jr,van Es MA,Pasterkamp RJ,Koppers M,Andersen PM,Estrada K,Rivadeneira F,Hofman A,Uitterlinden AG,van Damme P,Melki J,Meininger V,Shatunov A,Shaw CE,Leigh PN,Shaw PJ,Morrison KE,Fogh I,Chi? A,Traynor BJ,Czell D,Weber M,Heutink P,de Bakker PI,Silani V,Robberecht W,van den Berg LH,Veldink JH

A novel KIAA0196 (SPG8) mutation in a Chinese family with spastic paraplegia.
Chinese medical journal127(10)1987-9(2014)
Wang X,Yang Y,Wang X,Li C,Jia J

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WASHC5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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