SMG7 cDNA ORF clone, Homo sapiens(human)

The following SMG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu04620 NM_001174061.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu63457 XM_017002970.1
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu63457 XM_017002969.1
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu102343 NM_001350222.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu34882 NM_001375584.1
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu34886 NM_001375585.1
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu90882 XM_017002968.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu02898 NM_201568.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu07205 NM_173156.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu22107 NM_201569.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34879 NM_001350220.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34883 NM_001350221.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34883 NM_001331007.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu63455 NM_001350219.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu34879 XM_011510205.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34879 XM_005245649.4
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34882 XM_005245652.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34883 XM_011510206.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34884 XM_011510207.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34884 XM_005245653.5
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34886 XM_006711678.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu63454 XM_011510208.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu63455 XM_011510209.3
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu63456 XM_011510210.2
Latest version!
Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu34883 NM_001331007.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34883 NM_001350221.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34883 XM_011510206.2 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34884 XM_011510207.2 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu02898 NM_201568.2 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu04620 NM_001174061.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu34879 NM_001350220.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu90882 XM_017002968.1 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu63455 NM_001350219.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu22107 NM_201569.2 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu07205 NM_173156.2 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu34879 XM_011510205.2 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu34879 XM_005245649.3 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu63454 XM_011510208.2 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu102343 NM_001350222.1 Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu63455 XM_011510209.2 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu34884 XM_005245653.4 Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu04620
    Clone ID Related Accession (Same CDS sequence) NM_001174061.1 , NM_001174061.2
    Accession Version NM_001174061.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AK299178.1, AB085674.1 and CN479993.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant also contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT
    GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG
    TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA
    AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT
    AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA
    GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT
    TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT
    CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG
    CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA
    CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA
    GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT
    CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG
    CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT
    GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC
    CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG
    GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC
    CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA
    CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001167532.1
    CDS345..3782
    Translation

    Target ORF information:

    RefSeq Version NM_001174061.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001174061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT
    GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG
    TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA
    AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT
    AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA
    GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT
    TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT
    CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG
    CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA
    CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA
    GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT
    CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG
    CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT
    GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC
    CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG
    GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC
    CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA
    CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu04620
    Clone ID Related Accession (Same CDS sequence) NM_001174061.1 , NM_001174061.2
    Accession Version NM_001174061.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AK299178.1, AB085674.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001174061.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. This variant also contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT
    GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG
    TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA
    AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT
    AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA
    GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT
    TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT
    CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG
    CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA
    CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA
    GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT
    CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG
    CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT
    GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC
    CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG
    GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC
    CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA
    CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001167532.1
    CDS217..3654
    Translation

    Target ORF information:

    RefSeq Version NM_001174061.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001174061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT GAGAAGGGAC TATAGCAAAG GAATAACTGT AACTAAGAAT
    GATGGAAAGA AGGACAACAA CAAGAGGAAA ACTGAAACCA AGAAATGCAC CTTAGAAAAG
    TTACAGGAAA CAGGAAAGCA GAATGTGGCA GTGCAGGTAA AATCCCAGAC AGAACTAAGA
    AAGACTCCAG TGTCTGAAGC CAGAAAAACA CCTGTAACTC AAACCCCAAC TCAAGCAAGT
    AACTCCCAGT TCATCCCCAT TCATCACCCT GGAGCCTTCC CTCCTCTTCC CAGCAGGCCA
    GGGTTTCCGC CCCCAACATA TGTTATCCCC CCGCCTGTGG CATTTTCTAT GGGCTCAGGT
    TACACCTTCC CAGCTGGTGT TTCTGTCCCA GGAACCTTTC TTCAGCCTAC AGCTCACTCT
    CCAGCAGGAA ACCAGGTGCA AGCTGGGAAA CAGTCCCACA TTCCTTACAG CCAGCAACGG
    CCCTCTGGAC CAGGGCCAAT GAACCAGGGA CCTCAACAAT CACAGCCACC TTCCCAGCAA
    CCCCTTACAT CTTTACCAGC TCAGCCAACA GCACAGTCTA CAAGCCAGCT GCAGGTTCAA
    GCTCTAACTC AGCAACAACA ATCCCCTACA AAAGCTGTGC CGGCTTTGGG GAAAAGCCCG
    CCTCACCACT CTGGATTCCA GCAGTATCAA CAGGCAGATG CCTCCAAACA GCTGTGGAAT
    CCCCCTCAGG TTCAAGGCCC ATTAGGGAAA ATTATGCCTG TGAAACAGCC CTACTACCTT
    CAGACCCAAG ACCCCATAAA ACTGTTTGAG CCGTCATTGC AACCTCCTGT AATGCAGCAG
    CAGCCTCTAG AAAAAAAAAT GAAGCCTTTT CCCATGGAGC CATATAACCA TAATCCCTCA
    GAAGTCAAGG TCCCAGAATT CTACTGGGAT TCTTCCTACA GCATGGCTGA TAACAGATCT
    GTAATGGCAC AGCAAGCAAA CATAGACCGC AGGGGCAAAC GGTCACCAGG AGTCTTCCGT
    CCAGAGCAGG ATCCTGTACC CAGAATGCCG TTTGAGGACC CCAAGAGCTC CCCTCTGCTT
    CCTCCGGACC TGTTAAAGAG TCTGGCTGCC TTGGAGGAAG AGGAAGAGCT GATTTTTTCT
    AACCCTCCTG ATCTTTACCC GGCTCTGCTG GGGCCTCTCG CCTCTCTTCC TGGACGAAGC
    CTTTTTAAAT CCTTATTGGA GAAGCCCTCA GAGCTCATGT CACATTCATC CTCTTTCCTG
    TCCCTCACCG GATTCTCTCT CAATCAGGAA AGATACCCAA ATAATAGTAT GTTCAATGAG
    GTATATGGGA AAAACCTGAC ATCCAGCTCC AAAGCAGAAC TCAGTCCCTC AATGGCCCCC
    CAGGAAACAT CTCTGTATTC CCTTTTTGAA GGGACTCCGT GGTCTCCATC ACTTCCTGCC
    AGTTCAGATC ATTCAACACC AGCCAGCCAG TCTCCTCATT CCTCTAACCC AAGCAGCCTA
    CCCAGCTCTC CTCCAACACA CAACCATAAT TCTGTTCCAT TCTCCAATTT TGGACCCATT
    GGGACTCCAG ATAACAGGGA TAGAAGGACT GCAGATCGGT GGAAAACTGA TAAGCCAGCC
    ATGGGTGGGT TTGGCATTGA TTATCTCTCA GCAACGTCAT CCTCTGAGAG CAGTTGGCAT
    CAGGCCAGCA CTCCGAGTGG CACCTGGACA GGCCATGGCC CTTCCATGGA GGATTCCTCT
    GCTGTCCTCA TGGAAAGCCT AAAGTCTATC TGGTCCAGTT CCATGATGCA TCCTGGACCT
    TCTGCTCTGG AGCAGCTGTT AATGCAGCAG AAGCAGAAAC AGCAACGGGG ACAAGGCACC
    ATGAACCCTC CACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63457
    Clone ID Related Accession (Same CDS sequence) XM_017002969.1 , XM_017002970.1
    Accession Version XM_017002969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA
    GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC
    AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT
    CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA
    TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG
    CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT
    GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC
    AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA
    CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG
    TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA
    AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA
    CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA
    TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG
    TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA
    TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC
    CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA
    CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858458.1
    CDS1318..3876
    Translation

    Target ORF information:

    RefSeq Version XM_017002969.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA
    GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC
    AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT
    CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA
    TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG
    CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT
    GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC
    AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA
    CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG
    TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA
    AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA
    CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA
    TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG
    TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA
    TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC
    CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA
    CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63457
    Clone ID Related Accession (Same CDS sequence) XM_017002969.1 , XM_017002970.1
    Accession Version XM_017002970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA
    GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC
    AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT
    CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA
    TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG
    CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT
    GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC
    AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA
    CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG
    TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA
    AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA
    CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA
    TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG
    TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA
    TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC
    CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA
    CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858459.1
    CDS1242..3800
    Translation

    Target ORF information:

    RefSeq Version XM_017002970.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TGAGAAGGGA CTATAGCAAA
    GGAATAACTG TAACTAAGAA TGATGGAAAG AAGGACAACA ACAAGAGGAA AACTGAAACC
    AAGAAATGCA CCTTAGAAAA GTTACAGGAA ACAGGAAAGC AGAATGTGGC AGTGCAGGTA
    AAATCCCAGA CAGAACTAAG AAAGACTCCA GTGTCTGAAG CCAGAAAAAC ACCTGTAACT
    CAAACCCCAA CTCAAGCAAG TAACTCCCAG TTCATCCCCA TTCATCACCC TGGAGCCTTC
    CCTCCTCTTC CCAGCAGGCC AGGGTTTCCG CCCCCAACAT ATGTTATCCC CCCGCCTGTG
    GCATTTTCTA TGGGCTCAGG TTACACCTTC CCAGCTGGTG TTTCTGTCCC AGGAACCTTT
    CTTCAGCCTA CAGCTCACTC TCCAGCAGGA AACCAGGTGC AAGCTGGGAA ACAGTCCCAC
    ATTCCTTACA GCCAGCAACG GCCCTCTGGA CCAGGGCCAA TGAACCAGGG ACCTCAACAA
    TCACAGCCAC CTTCCCAGCA ACCCCTTACA TCTTTACCAG CTCAGCCAAC AGCACAGTCT
    ACAAGCCAGC TGCAGGTTCA AGCTCTAACT CAGCAACAAC AATCCCCTAC AAAAGCTGTG
    CCGGCTTTGG GGAAAAGCCC GCCTCACCAC TCTGGATTCC AGCAGTATCA ACAGGCAGAT
    GCCTCCAAAC AGCTGTGGAA TCCCCCTCAG GTTCAAGGCC CATTAGGGAA AATTATGCCT
    GTGAAACAGC CCTACTACCT TCAGACCCAA GACCCCATAA AACTGTTTGA GCCGTCATTG
    CAACCTCCTG TAATGCAGCA GCAGCCTCTA GAAAAAAAAA TGAAGCCTTT TCCCATGGAG
    CCATATAACC ATAATCCCTC AGAAGTCAAG GTCCCAGAAT TCTACTGGGA TTCTTCCTAC
    AGCATGGCTG ATAACAGATC TGTAATGGCA CAGCAAGCAA ACATAGACCG CAGGGGCAAA
    CGGTCACCAG GAGTCTTCCG TCCAGAGCAG GATCCTGTAC CCAGAATGCC GTTTGAGGAC
    CCCAAGAGCT CCCCTCTGCT TCCTCCGGAC CTGTTAAAGA GTCTGGCTGC CTTGGAGGAA
    GAGGAAGAGC TGATTTTTTC TAACCCTCCT GATCTTTACC CGGCTCTGCT GGGGCCTCTC
    GCCTCTCTTC CTGGACGAAG CCTTTTTAAA TCCTTATTGG AGAAGCCCTC AGAGCTCATG
    TCACATTCAT CCTCTTTCCT GTCCCTCACC GGATTCTCTC TCAATCAGGA AAGATACCCA
    AATAATAGTA TGTTCAATGA GGTATATGGG AAAAACCTGA CATCCAGCTC CAAAGCAGAA
    CTCAGTCCCT CAATGGCCCC CCAGGAAACA TCTCTGTATT CCCTTTTTGA AGGGACTCCG
    TGGTCTCCAT CACTTCCTGC CAGTTCAGAT CATTCAACAC CAGCCAGCCA GTCTCCTCAT
    TCCTCTAACC CAAGCAGCCT ACCCAGCTCT CCTCCAACAC ACAACCATAA TTCTGTTCCA
    TTCTCCAATT TTGGACCCAT TGGGACTCCA GATAACAGGG ATAGAAGGAC TGCAGATCGG
    TGGAAAACTG ATAAGCCAGC CATGGGTGGG TTTGGCATTG ATTATCTCTC AGCAACGTCA
    TCCTCTGAGA GCAGTTGGCA TCAGGCCAGC ACTCCGAGTG GCACCTGGAC AGGCCATGGC
    CCTTCCATGG AGGATTCCTC TGCTGTCCTC ATGGAAAGCC TAAAGTCTAT CTGGTCCAGT
    TCCATGATGC ATCCTGGACC TTCTGCTCTG GAGCAGCTGT TAATGCAGCA GAAGCAGAAA
    CAGCAACGGG GACAAGGCAC CATGAACCCT CCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102343
    Clone ID Related Accession (Same CDS sequence) NM_001350222.2 , NM_001350222.1
    Accession Version NM_001350222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, DA773831.1, AB085674.1, BC036381.1, AK299178.1, DA629659.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350222.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2850010.1, SRR1660807.228673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA
    AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG
    CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG
    CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT
    CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC
    CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG
    CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA
    TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC
    CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337151.1
    CDS1190..3610
    Translation

    Target ORF information:

    RefSeq Version NM_001350222.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA
    AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG
    CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG
    CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT
    CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC
    CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG
    CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA
    TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC
    CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102343
    Clone ID Related Accession (Same CDS sequence) NM_001350222.2 , NM_001350222.1
    Accession Version NM_001350222.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, DA773831.1, AB085674.1, BC036381.1, AK299178.1, DA629659.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002971.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.228673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA
    AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG
    CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG
    CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT
    CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC
    CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG
    CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA
    TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC
    CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337151.1
    CDS1318..3738
    Translation

    Target ORF information:

    RefSeq Version NM_001350222.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGTCTTTTC TTGGCATCCT GTGCAAGTGT CCTCTACAGA ATGAGTCTCA GGAGGAGTCC 
    TACAATGCCT ATCCTCTTCC AGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCAGACCC
    AGGGTCTTTC AGGAGGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GTTGATTTCT
    CTTCTGAATA GTTTCCATCC CCATGAAGAG GACCTCTCAA GTATTAGTGC GACACCACTT
    CCAGAGGAGT TTGAATTACA AGGATTTTTG GCATTGAGAC CTTCTTTCAG GAACTTGGAT
    TTTTCCAAAG GTCACCAGGG TATTACAGGG GACAAAGAAG GCCAGCAACG ACGAATACGA
    CAGCAACGCT TGATCTCTAT AGGCAAATGG ATTGCTGATA ATCAGCCAAG GCTGATTCAG
    TGTGAAAATG AGGTAGGGAA ATTGTTGTTT ATCACAGAAA TCCCAGAATT AATACTGGAA
    GACCCCAGTG AAGCCAAAGA GAACCTCATT CTGCAAGAAA CATCTGTGAT AGAGTCGCTG
    GCTGCAGATG GGAGCCCAGG GCTAAAATCA GTGCTATCTA CAAGCCGAAA TTTAAGCAAC
    AACTGTGACA CAGGAGAGAA GCCAGTGGTT ACCTTCAAAG AAAACATTAA GACACGAGAA
    GTGAACAGAG ACCAAGGAAG AAGTTTTCCT CCCAAAGAGG TAAAATCCCA GACAGAACTA
    AGAAAGACTC CAGTGTCTGA AGCCAGAAAA ACACCTGTAA CTCAAACCCC AACTCAAGCA
    AGTAACTCCC AGTTCATCCC CATTCATCAC CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG
    CCAGGGTTTC CGCCCCCAAC ATATGTTATC CCCCCGCCTG TGGCATTTTC TATGGGCTCA
    GGTTACACCT TCCCAGCTGG TGTTTCTGTC CCAGGAACCT TTCTTCAGCC TACAGCTCAC
    TCTCCAGCAG GAAACCAGGT GCAAGCTGGG AAACAGTCCC ACATTCCTTA CAGCCAGCAA
    CGGCCCTCTG GACCAGGGCC AATGAACCAG GGACCTCAAC AATCACAGCC ACCTTCCCAG
    CAACCCCTTA CATCTTTACC AGCTCAGCCA ACAGCACAGT CTACAAGCCA GCTGCAGGTT
    CAAGCTCTAA CTCAGCAACA ACAATCCCCT ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC
    CCGCCTCACC ACTCTGGATT CCAGCAGTAT CAACAGGCAG ATGCCTCCAA ACAGCTGTGG
    AATCCCCCTC AGGTTCAAGG CCCATTAGGG AAAATTATGC CTGTGAAACA GCCCTACTAC
    CTTCAGACCC AAGACCCCAT AAAACTGTTT GAGCCGTCAT TGCAACCTCC TGTAATGCAG
    CAGCAGCCTC TAGAAAAAAA AATGAAGCCT TTTCCCATGG AGCCATATAA CCATAATCCC
    TCAGAAGTCA AGGTCCCAGA ATTCTACTGG GATTCTTCCT ACAGCATGGC TGATAACAGA
    TCTGTAATGG CACAGCAAGC AAACATAGAC CGCAGGGGCA AACGGTCACC AGGAGTCTTC
    CGTCCAGAGC AGGATCCTGT ACCCAGAATG CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG
    CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT GCCTTGGAGG AAGAGGAAGA GCTGATTTTT
    TCTAACCCTC CTGATCTTTA CCCGGCTCTG CTGGGGCCTC TCGCCTCTCT TCCTGGACGA
    AGCCTTTTTA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34882
    Clone ID Related Accession (Same CDS sequence) XM_005245652.3 , NM_001375584.1
    Accession Version NM_001375584.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 10
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Oct 29, 2019 this sequence version replaced XM_005245652.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.221341.1, SRR1660807.81080.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC
    AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG
    GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC
    AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA
    GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362513.1
    CDS123..3686
    Translation

    Target ORF information:

    RefSeq Version NM_001375584.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375584.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC
    AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG
    GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC
    AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA
    GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34886
    Clone ID Related Accession (Same CDS sequence) NM_001375585.1 , XM_006711678.3
    Accession Version NM_001375585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 11
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Oct 29, 2019 this sequence version replaced XM_006711678.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2891567.1, SRR1803615.167462.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG
    CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001362514.1
    CDS123..3548
    Translation

    Target ORF information:

    RefSeq Version NM_001375585.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001375585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG
    CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90882
    Clone ID Related Accession (Same CDS sequence) XM_017002968.1 , XM_017002968.2
    Accession Version XM_017002968.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858457.1
    CDS220..3369
    Translation

    Target ORF information:

    RefSeq Version XM_017002968.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90882
    Clone ID Related Accession (Same CDS sequence) XM_017002968.1 , XM_017002968.2
    Accession Version XM_017002968.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858457.1
    CDS214..3363
    Translation

    Target ORF information:

    RefSeq Version XM_017002968.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002968.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02898
    Clone ID Related Accession (Same CDS sequence) NM_201568.3 , NM_201568.2
    Accession Version NM_201568.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, D87437.2 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_201568.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2287091.1, SRR7346977.2824553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA
    CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT
    AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC
    AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG
    TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC
    TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC
    TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG
    AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC
    TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT
    TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC
    ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG
    CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963862.1
    CDS123..3398
    Translation

    Target ORF information:

    RefSeq Version NM_201568.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201568.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA
    CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT
    AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC
    AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG
    TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC
    TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC
    TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG
    AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC
    TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT
    TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC
    ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG
    CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02898
    Clone ID Related Accession (Same CDS sequence) NM_201568.3 , NM_201568.2
    Accession Version NM_201568.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, D87437.2 and CN479993.1. On Mar 23, 2010 this sequence version replaced NM_201568.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87437.2, SRR1803614.66164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA
    CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT
    AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC
    AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG
    TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC
    TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC
    TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG
    AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC
    TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT
    TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC
    ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG
    CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963862.1
    CDS251..3526
    Translation

    Target ORF information:

    RefSeq Version NM_201568.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201568.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGAAATCC TTATTGGAGA AGCCCTCAGA GCTCATGTCA
    CATTCATCCT CTTTCCTGTC CCTCACCGGA TTCTCTCTCA ATCAGGAAAG ATACCCAAAT
    AATAGTATGT TCAATGAGGT ATATGGGAAA AACCTGACAT CCAGCTCCAA AGCAGAACTC
    AGTCCCTCAA TGGCCCCCCA GGAAACATCT CTGTATTCCC TTTTTGAAGG GACTCCGTGG
    TCTCCATCAC TTCCTGCCAG TTCAGATCAT TCAACACCAG CCAGCCAGTC TCCTCATTCC
    TCTAACCCAA GCAGCCTACC CAGCTCTCCT CCAACACACA ACCATAATTC TGTTCCATTC
    TCCAATTTTG GACCCATTGG GACTCCAGAT AACAGGGATA GAAGGACTGC AGATCGGTGG
    AAAACTGATA AGCCAGCCAT GGGTGGGTTT GGCATTGATT ATCTCTCAGC AACGTCATCC
    TCTGAGAGCA GTTGGCATCA GGCCAGCACT CCGAGTGGCA CCTGGACAGG CCATGGCCCT
    TCCATGGAGG ATTCCTCTGC TGTCCTCATG GAAAGCCTAA AGTCTATCTG GTCCAGTTCC
    ATGATGCATC CTGGACCTTC TGCTCTGGAG CAGCTGTTAA TGCAGCAGAA GCAGAAACAG
    CAACGGGGAC AAGGCACCAT GAACCCTCCA CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07205
    Clone ID Related Accession (Same CDS sequence) NM_173156.2 , NM_173156.3
    Accession Version NM_173156.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, AY386363.1 and CN479993.1. On Mar 23, 2010 this sequence version replaced NM_173156.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.233309.1, AB085674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG
    CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT
    CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC
    AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT
    TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC
    AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC
    CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA
    AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT
    CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC
    TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG
    TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG
    CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_775179.1
    CDS251..3664
    Translation

    Target ORF information:

    RefSeq Version NM_173156.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG
    CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT
    CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC
    AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT
    TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC
    AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC
    CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA
    AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT
    CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC
    TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG
    TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG
    CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu07205
    Clone ID Related Accession (Same CDS sequence) NM_173156.2 , NM_173156.3
    Accession Version NM_173156.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3414bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, DC380923.1, AY386363.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_173156.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2306630.1, SRR7346977.2233726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG
    CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT
    CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC
    AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT
    TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC
    AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC
    CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA
    AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT
    CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC
    TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG
    TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG
    CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_775179.1
    CDS123..3536
    Translation

    Target ORF information:

    RefSeq Version NM_173156.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173156.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGAAATCCTT ATTGGAGAAG
    CCCTCAGAGC TCATGTCACA TTCATCCTCT TTCCTGTCCC TCACCGGATT CTCTCTCAAT
    CAGGAAAGAT ACCCAAATAA TAGTATGTTC AATGAGGTAT ATGGGAAAAA CCTGACATCC
    AGCTCCAAAG CAGAACTCAG TCCCTCAATG GCCCCCCAGG AAACATCTCT GTATTCCCTT
    TTTGAAGGGA CTCCGTGGTC TCCATCACTT CCTGCCAGTT CAGATCATTC AACACCAGCC
    AGCCAGTCTC CTCATTCCTC TAACCCAAGC AGCCTACCCA GCTCTCCTCC AACACACAAC
    CATAATTCTG TTCCATTCTC CAATTTTGGA CCCATTGGGA CTCCAGATAA CAGGGATAGA
    AGGACTGCAG ATCGGTGGAA AACTGATAAG CCAGCCATGG GTGGGTTTGG CATTGATTAT
    CTCTCAGCAA CGTCATCCTC TGAGAGCAGT TGGCATCAGG CCAGCACTCC GAGTGGCACC
    TGGACAGGCC ATGGCCCTTC CATGGAGGAT TCCTCTGCTG TCCTCATGGA AAGCCTAAAG
    TCTATCTGGT CCAGTTCCAT GATGCATCCT GGACCTTCTG CTCTGGAGCA GCTGTTAATG
    CAGCAGAAGC AGAAACAGCA ACGGGGACAA GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22107
    Clone ID Related Accession (Same CDS sequence) NM_201569.2 , NM_201569.3
    Accession Version NM_201569.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, BC036381.1, AB085674.1, AK299178.1, AL137800.12 and CN479993.1. This sequence is a reference standard in the RefSeqGene project. On Mar 23, 2010 this sequence version replaced NM_201569.1. Transcript Variant: This variant (4) has multiple differences in the coding region compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA
    GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC
    AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG
    GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963863.2
    CDS251..3787
    Translation

    Target ORF information:

    RefSeq Version NM_201569.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA
    GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC
    AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG
    GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22107
    Clone ID Related Accession (Same CDS sequence) NM_201569.2 , NM_201569.3
    Accession Version NM_201569.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, BC036381.1, AB085674.1, AK299178.1, AL137800.12 and CN479993.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_201569.2. Transcript Variant: This variant (4) has multiple differences in the coding region compared to variant 1, one of which results in a translational frameshift. The resulting protein (isoform 4) has a distinct C-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA
    GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC
    AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG
    GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963863.2
    CDS123..3659
    Translation

    Target ORF information:

    RefSeq Version NM_201569.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201569.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGAAGCAACA GCATGGGGTC CAGCAGTTGG GGCCCAAAAG ACAGTCTGAA
    GAGGAAGGAA GCAGCAGTAT CTGCGTAGCC CACAGAGGGC CCAGGCCCCT GCCCAGCTGC
    AGTCTCCCAG CCTCCACTTT CAGAGTGAAA TTCAAGGCAG CACGGACATG TGCCCATCAG
    GCACAGAAGA AAACACGACG TCGTCCATTT TGGAAGAGAC GAAAGAAAGG AAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version NM_001350220.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, CR995963.1, DA238539.1, DA057117.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_005245648.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.223290.1, SRR1803612.198442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337149.1
    CDS440..4090
    Translation

    Target ORF information:

    RefSeq Version NM_001350220.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version NM_001350220.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, CR995963.1, DA238539.1, DA057117.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350220.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.223290.1, SRR1803612.198442.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337149.1
    CDS312..3962
    Translation

    Target ORF information:

    RefSeq Version NM_001350220.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350220.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version NM_001331007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Jun 2, 2019 this sequence version replaced NM_001331007.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, uses an alternate in-frame splice junction, and contains an alternate in-frame exon compared to variant 1. The resulting isoform (6) has a longer and distinct N-terminus, contains an alternate internal segment, and lacks another alternate internal segment compared to isoform 1. Variants 6 and 9 encode the same protein (isoform 6). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.202323.1, SRR1803611.163447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317936.1
    CDS175..3687
    Translation

    Target ORF information:

    RefSeq Version NM_001331007.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001331007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version NM_001350221.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, DA096344.1 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002966.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.147809.1, SRR1803614.253492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337150.1
    CDS440..3952
    Translation

    Target ORF information:

    RefSeq Version NM_001350221.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version NM_001331007.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL449223.7 and AL137800.12. On Sep 3, 2016 this sequence version replaced XM_006711676.3. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, uses an alternate in-frame splice junction, and contains an alternate in-frame exon compared to variant 1. The resulting isoform (6) has a longer and distinct N-terminus, contains an alternate internal segment, and lacks another alternate internal segment compared to isoform 1. Variants 6 and 9 encode the same protein (isoform 6). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.172321.1, SRR1803611.163447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317936.1
    CDS303..3815
    Translation

    Target ORF information:

    RefSeq Version NM_001331007.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001331007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version NM_001350221.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2, DA096344.1 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350221.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.147809.1, SRR1803614.253492.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337150.1
    CDS312..3824
    Translation

    Target ORF information:

    RefSeq Version NM_001350221.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350221.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63455
    Clone ID Related Accession (Same CDS sequence) NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
    Accession Version NM_001350219.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-27
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2 and CN479993.1. On Apr 10, 2017 this sequence version replaced XM_017002967.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337148.1
    CDS440..3802
    Translation

    Target ORF information:

    RefSeq Version NM_001350219.1
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63455
    Clone ID Related Accession (Same CDS sequence) NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
    Accession Version NM_001350219.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product protein SMG7 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB223038.1, AB085674.1, AL137800.12, DC341780.1, D87437.2 and CN479993.1. On Jun 2, 2019 this sequence version replaced NM_001350219.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001337148.1
    CDS312..3674
    Translation

    Target ORF information:

    RefSeq Version NM_001350219.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001350219.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version XM_011510205.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508507.1
    CDS1027..4677
    Translation

    Target ORF information:

    RefSeq Version XM_011510205.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version XM_005245649.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245706.1
    CDS712..4362
    Translation

    Target ORF information:

    RefSeq Version XM_005245649.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245649.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version XM_011510205.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508507.1
    CDS996..4646
    Translation

    Target ORF information:

    RefSeq Version XM_011510205.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510205.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34879
    Clone ID Related Accession (Same CDS sequence) NM_001350220.1 , XM_011510205.2 , NM_001350220.2 , XM_005245649.3 , XM_011510205.3 , XM_005245649.4
    Accession Version XM_005245649.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245706.1
    CDS681..4331
    Translation

    Target ORF information:

    RefSeq Version XM_005245649.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245649.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGG ACCCCAAGAG CTCCCCTCTG CTTCCTCCGG ACCTGTTAAA GAGTCTGGCT
    GCCTTGGAGG AAGAGGAAGA GCTGATTTTT TCTAACCCTC CTGATCTTTA CCCGGCTCTG
    CTGGGGCCTC TCGCCTCTCT TCCTGGACGA AGCCTTTTTA AATCCTTATT GGAGAAGCCC
    TCAGAGCTCA TGTCACATTC ATCCTCTTTC CTGTCCCTCA CCGGATTCTC TCTCAATCAG
    GAAAGATACC CAAATAATAG TATGTTCAAT GAGGTATATG GGAAAAACCT GACATCCAGC
    TCCAAAGCAG AACTCAGTCC CTCAATGGCC CCCCAGGAAA CATCTCTGTA TTCCCTTTTT
    GAAGGGACTC CGTGGTCTCC ATCACTTCCT GCCAGTTCAG ATCATTCAAC ACCAGCCAGC
    CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC CTACCCAGCT CTCCTCCAAC ACACAACCAT
    AATTCTGTTC CATTCTCCAA TTTTGGACCC ATTGGGACTC CAGATAACAG GGATAGAAGG
    ACTGCAGATC GGTGGAAAAC TGATAAGCCA GCCATGGGTG GGTTTGGCAT TGATTATCTC
    TCAGCAACGT CATCCTCTGA GAGCAGTTGG CATCAGGCCA GCACTCCGAG TGGCACCTGG
    ACAGGCCATG GCCCTTCCAT GGAGGATTCC TCTGCTGTCC TCATGGAAAG CCTAAAGTCT
    ATCTGGTCCA GTTCCATGAT GCATCCTGGA CCTTCTGCTC TGGAGCAGCT GTTAATGCAG
    CAGAAGCAGA AACAGCAACG GGGACAAGGC ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34882
    Clone ID Related Accession (Same CDS sequence) XM_005245652.3 , NM_001375584.1
    Accession Version XM_005245652.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245652.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC
    AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG
    GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC
    AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA
    GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245709.1
    CDS120..3683
    Translation

    Target ORF information:

    RefSeq Version XM_005245652.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245652.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTGAGA AGGGACTATA GCAAAGGAAT AACTGTAACT
    AAGAATGATG GAAAGAAGGA CAACAACAAG AGGAAAACTG AAACCAAGAA ATGCACCTTA
    GAAAAGTTAC AGGAAACAGG AAAGCAGAAT GTGGCAGTGC AGGTAAAATC CCAGACAGAA
    CTAAGAAAGA CTCCAGTGTC TGAAGCCAGA AAAACACCTG TAACTCAAAC CCCAACTCAA
    GCAAGTAACT CCCAGTTCAT CCCCATTCAT CACCCTGGAG CCTTCCCTCC TCTTCCCAGC
    AGGCCAGGGT TTCCGCCCCC AACATATGTT ATCCCCCCGC CTGTGGCATT TTCTATGGGC
    TCAGGTTACA CCTTCCCAGC TGGTGTTTCT GTCCCAGGAA CCTTTCTTCA GCCTACAGCT
    CACTCTCCAG CAGGAAACCA GGTGCAAGCT GGGAAACAGT CCCACATTCC TTACAGCCAG
    CAACGGCCCT CTGGACCAGG GCCAATGAAC CAGGGACCTC AACAATCACA GCCACCTTCC
    CAGCAACCCC TTACATCTTT ACCAGCTCAG CCAACAGCAC AGTCTACAAG CCAGCTGCAG
    GTTCAAGCTC TAACTCAGCA ACAACAATCC CCTACAAAAG CTGTGCCGGC TTTGGGGAAA
    AGCCCGCCTC ACCACTCTGG ATTCCAGCAG TATCAACAGG CAGATGCCTC CAAACAGCTG
    TGGAATCCCC CTCAGGTTCA AGGCCCATTA GGGAAAATTA TGCCTGTGAA ACAGCCCTAC
    TACCTTCAGA CCCAAGACCC CATAAAACTG TTTGAGCCGT CATTGCAACC TCCTGTAATG
    CAGCAGCAGC CTCTAGAAAA AAAAATGAAG CCTTTTCCCA TGGAGCCATA TAACCATAAT
    CCCTCAGAAG TCAAGGTCCC AGAATTCTAC TGGGATTCTT CCTACAGCAT GGCTGATAAC
    AGATCTGTAA TGGCACAGCA AGCAAACATA GACCGCAGGG GCAAACGGTC ACCAGGAGTC
    TTCCGTCCAG AGCAGGATCC TGTACCCAGA ATGCCGTTTG AGGACCCCAA GAGCTCCCCT
    CTGCTTCCTC CGGACCTGTT AAAGAGTCTG GCTGCCTTGG AGGAAGAGGA AGAGCTGATT
    TTTTCTAACC CTCCTGATCT TTACCCGGCT CTGCTGGGGC CTCTCGCCTC TCTTCCTGGA
    CGAAGCCTTT TTAAATCCTT ATTGGAGAAG CCCTCAGAGC TCATGTCACA TTCATCCTCT
    TTCCTGTCCC TCACCGGATT CTCTCTCAAT CAGGAAAGAT ACCCAAATAA TAGTATGTTC
    AATGAGGTAT ATGGGAAAAA CCTGACATCC AGCTCCAAAG CAGAACTCAG TCCCTCAATG
    GCCCCCCAGG AAACATCTCT GTATTCCCTT TTTGAAGGGA CTCCGTGGTC TCCATCACTT
    CCTGCCAGTT CAGATCATTC AACACCAGCC AGCCAGTCTC CTCATTCCTC TAACCCAAGC
    AGCCTACCCA GCTCTCCTCC AACACACAAC CATAATTCTG TTCCATTCTC CAATTTTGGA
    CCCATTGGGA CTCCAGATAA CAGGGATAGA AGGACTGCAG ATCGGTGGAA AACTGATAAG
    CCAGCCATGG GTGGGTTTGG CATTGATTAT CTCTCAGCAA CGTCATCCTC TGAGAGCAGT
    TGGCATCAGG CCAGCACTCC GAGTGGCACC TGGACAGGCC ATGGCCCTTC CATGGAGGAT
    TCCTCTGCTG TCCTCATGGA AAGCCTAAAG TCTATCTGGT CCAGTTCCAT GATGCATCCT
    GGACCTTCTG CTCTGGAGCA GCTGTTAATG CAGCAGAAGC AGAAACAGCA ACGGGGACAA
    GGCACCATGA ACCCTCCACA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version XM_011510206.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508508.1
    CDS1027..4539
    Translation

    Target ORF information:

    RefSeq Version XM_011510206.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34883
    Clone ID Related Accession (Same CDS sequence) XM_011510206.2 , NM_001331007.2 , NM_001350221.1 , NM_001331007.1 , NM_001350221.2 , XM_011510206.3
    Accession Version XM_011510206.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508508.1
    CDS996..4508
    Translation

    Target ORF information:

    RefSeq Version XM_011510206.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510206.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GGACCCCAAG
    AGCTCCCCTC TGCTTCCTCC GGACCTGTTA AAGAGTCTGG CTGCCTTGGA GGAAGAGGAA
    GAGCTGATTT TTTCTAACCC TCCTGATCTT TACCCGGCTC TGCTGGGGCC TCTCGCCTCT
    CTTCCTGGAC GAAGCCTTTT TAAATCCTTA TTGGAGAAGC CCTCAGAGCT CATGTCACAT
    TCATCCTCTT TCCTGTCCCT CACCGGATTC TCTCTCAATC AGGAAAGATA CCCAAATAAT
    AGTATGTTCA ATGAGGTATA TGGGAAAAAC CTGACATCCA GCTCCAAAGC AGAACTCAGT
    CCCTCAATGG CCCCCCAGGA AACATCTCTG TATTCCCTTT TTGAAGGGAC TCCGTGGTCT
    CCATCACTTC CTGCCAGTTC AGATCATTCA ACACCAGCCA GCCAGTCTCC TCATTCCTCT
    AACCCAAGCA GCCTACCCAG CTCTCCTCCA ACACACAACC ATAATTCTGT TCCATTCTCC
    AATTTTGGAC CCATTGGGAC TCCAGATAAC AGGGATAGAA GGACTGCAGA TCGGTGGAAA
    ACTGATAAGC CAGCCATGGG TGGGTTTGGC ATTGATTATC TCTCAGCAAC GTCATCCTCT
    GAGAGCAGTT GGCATCAGGC CAGCACTCCG AGTGGCACCT GGACAGGCCA TGGCCCTTCC
    ATGGAGGATT CCTCTGCTGT CCTCATGGAA AGCCTAAAGT CTATCTGGTC CAGTTCCATG
    ATGCATCCTG GACCTTCTGC TCTGGAGCAG CTGTTAATGC AGCAGAAGCA GAAACAGCAA
    CGGGGACAAG GCACCATGAA CCCTCCACAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34884
    Clone ID Related Accession (Same CDS sequence) XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
    Accession Version XM_011510207.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508509.1
    CDS892..4401
    Translation

    Target ORF information:

    RefSeq Version XM_011510207.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510207.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34884
    Clone ID Related Accession (Same CDS sequence) XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
    Accession Version XM_011510207.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508509.1
    CDS861..4370
    Translation

    Target ORF information:

    RefSeq Version XM_011510207.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510207.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34884
    Clone ID Related Accession (Same CDS sequence) XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
    Accession Version XM_005245653.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245653.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245710.1
    CDS276..3785
    Translation

    Target ORF information:

    RefSeq Version XM_005245653.4
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245653.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34884
    Clone ID Related Accession (Same CDS sequence) XM_011510207.2 , XM_011510207.3 , XM_005245653.4 , XM_005245653.5
    Accession Version XM_005245653.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245653.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245710.1
    CDS245..3754
    Translation

    Target ORF information:

    RefSeq Version XM_005245653.5
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245653.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGACAGATT CTAAGCTGGG TCCAGCTGAA GTCTGGACAT CCAGGCAGGC TCTGCAGGAC 
    CTGTACCAGA AAATGCTAGT TACCGATTTG GAATACGCTT TAGACAAGAA AGTAGAACAG
    GATCTCTGGA ATCACGCCTT TAAGAATCAG ATCACAACAC TGCAAGGCCA GGCAAAGAAT
    CGAGCAAATC CGAATCGGAG TGAAGTTCAG GCAAACCTTT CTCTGTTCCT AGAGGCAGCT
    AGTGGCTTCT ATACTCAGTT ATTACAAGAA CTGTGTACAG TATTTAATGT AGATTTACCA
    TGCCGTGTGA AGTCTTCCCA ATTGGGAATT ATCAGCAATA AACAGACGCA TACCAGCGCC
    ATAGTGAAGC CACAGTCTAG CTCCTGTTCC TATATCTGCC AGCACTGCCT CGTCCACCTT
    GGAGACATTG CTCGATACAG AAACCAGACC AGCCAGGCAG AGTCCTACTA TAGGCATGCA
    GCTCAGCTTG TCCCCTCCAA TGGTCAGCCT TATAATCAGT TGGCTATCTT AGCTTCTTCC
    AAAGGAGACC ATCTGACCAC AATTTTCTAC TACTGCAGAA GCATTGCTGT GAAGTTCCCT
    TTCCCAGCTG CCTCCACTAA TCTGCAAAAA GCACTTTCTA AAGCACTGGA AAGCCGAGAT
    GAGGTGAAAA CCAAGTGGGG TGTTTCTGAC TTCATCAAGG CCTTTATTAA ATTCCACGGT
    CATGTGTACC TGAGTAAGAG CTTGGAAAAG TTGAGCCCTC TTCGAGAGAA ATTGGAAGAA
    CAGTTTAAGA GGCTGCTATT CCAAAAAGCT TTCAACTCTC AGCAGTTAGT TCATGTCACT
    GTCATTAACC TGTTTCAACT TCATCACCTT CGTGACTTTA GCAATGAAAC CGAGCAGCAC
    ACTTATAGCC AAGATGAGCA GCTATGTTGG ACACAGTTGC TGGCCCTCTT TATGTCTTTT
    CTTGGCATCC TGTGCAAGTG TCCTCTACAG AATGAGTCTC AGGAGGAGTC CTACAATGCC
    TATCCTCTTC CAGCAGTCAA GGTCTCCATG GACTGGCTAA GACTCAGACC CAGGGTCTTT
    CAGGAGGCAG TGGTGGATGA AAGACAGTAC ATTTGGCCCT GGTTGATTTC TCTTCTGAAT
    AGTTTCCATC CCCATGAAGA GGACCTCTCA AGTATTAGTG CGACACCACT TCCAGAGGAG
    TTTGAATTAC AAGGATTTTT GGCATTGAGA CCTTCTTTCA GGAACTTGGA TTTTTCCAAA
    GGTCACCAGG GTATTACAGG GGACAAAGAA GGCCAGCAAC GACGAATACG ACAGCAACGC
    TTGATCTCTA TAGGCAAATG GATTGCTGAT AATCAGCCAA GGCTGATTCA GTGTGAAAAT
    GAGGTAGGGA AATTGTTGTT TATCACAGAA ATCCCAGAAT TAATACTGGA AGACCCCAGT
    GAAGCCAAAG AGAACCTCAT TCTGCAAGAA ACATCTGTGA TAGAGTCGCT GGCTGCAGAT
    GGGAGCCCAG GGCTAAAATC AGTGCTATCT ACAAGCCGAA ATTTAAGCAA CAACTGTGAC
    ACAGGAGAGA AGCCAGTGGT TACCTTCAAA GAAAACATTA AGACACGAGA AGTGAACAGA
    GACCAAGGAA GAAGTTTTCC TCCCAAAGAG GTGAGAAGGG ACTATAGCAA AGGAATAACT
    GTAACTAAGA ATGATGGAAA GAAGGACAAC AACAAGAGGA AAACTGAAAC CAAGAAATGC
    ACCTTAGAAA AGTTACAGGA AACAGGAAAG CAGAATGTGG CAGTGCAGGT AAAATCCCAG
    ACAGAACTAA GAAAGACTCC AGTGTCTGAA GCCAGAAAAA CACCTGTAAC TCAAACCCCA
    ACTCAAGCAA GTAACTCCCA GTTCATCCCC ATTCATCACC CTGGAGCCTT CCCTCCTCTT
    CCCAGCAGGC CAGGGTTTCC GCCCCCAACA TATGTTATCC CCCCGCCTGT GGCATTTTCT
    ATGGGCTCAG GTTACACCTT CCCAGCTGGT GTTTCTGTCC CAGGAACCTT TCTTCAGCCT
    ACAGCTCACT CTCCAGCAGG AAACCAGGTG CAAGCTGGGA AACAGTCCCA CATTCCTTAC
    AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA ATGAACCAGG GACCTCAACA ATCACAGCCA
    CCTTCCCAGC AACCCCTTAC ATCTTTACCA GCTCAGCCAA CAGCACAGTC TACAAGCCAG
    CTGCAGGTTC AAGCTCTAAC TCAGCAACAA CAATCCCCTA CAAAAGCTGT GCCGGCTTTG
    GGGAAAAGCC CGCCTCACCA CTCTGGATTC CAGCAGTATC AACAGGCAGA TGCCTCCAAA
    CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC CCATTAGGGA AAATTATGCC TGTGAAACAG
    CCCTACTACC TTCAGACCCA AGACCCCATA AAACTGTTTG AGCCGTCATT GCAACCTCCT
    GTAATGCAGC AGCAGCCTCT AGAAAAAAAA ATGAAGCCTT TTCCCATGGA GCCATATAAC
    CATAATCCCT CAGAAGTCAA GGTCCCAGAA TTCTACTGGG ATTCTTCCTA CAGCATGGCT
    GATAACAGAT CTGTAATGGC ACAGCAAGCA AACATAGACC GCAGGGGCAA ACGGTCACCA
    GGAGTCTTCC GTCCAGAGCA GGATCCTGTA CCCAGAATGC CGTTTGAGGA CCCCAAGAGC
    TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG
    CTGATTTTTT CTAACCCTCC TGATCTTTAC CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT
    CCTGGACGAA GCCTTTTTAA ATCCTTATTG GAGAAGCCCT CAGAGCTCAT GTCACATTCA
    TCCTCTTTCC TGTCCCTCAC CGGATTCTCT CTCAATCAGG AAAGATACCC AAATAATAGT
    ATGTTCAATG AGGTATATGG GAAAAACCTG ACATCCAGCT CCAAAGCAGA ACTCAGTCCC
    TCAATGGCCC CCCAGGAAAC ATCTCTGTAT TCCCTTTTTG AAGGGACTCC GTGGTCTCCA
    TCACTTCCTG CCAGTTCAGA TCATTCAACA CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC
    CCAAGCAGCC TACCCAGCTC TCCTCCAACA CACAACCATA ATTCTGTTCC ATTCTCCAAT
    TTTGGACCCA TTGGGACTCC AGATAACAGG GATAGAAGGA CTGCAGATCG GTGGAAAACT
    GATAAGCCAG CCATGGGTGG GTTTGGCATT GATTATCTCT CAGCAACGTC ATCCTCTGAG
    AGCAGTTGGC ATCAGGCCAG CACTCCGAGT GGCACCTGGA CAGGCCATGG CCCTTCCATG
    GAGGATTCCT CTGCTGTCCT CATGGAAAGC CTAAAGTCTA TCTGGTCCAG TTCCATGATG
    CATCCTGGAC CTTCTGCTCT GGAGCAGCTG TTAATGCAGC AGAAGCAGAA ACAGCAACGG
    GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34886
    Clone ID Related Accession (Same CDS sequence) NM_001375585.1 , XM_006711678.3
    Accession Version XM_006711678.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG
    CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006711741.1
    CDS120..3545
    Translation

    Target ORF information:

    RefSeq Version XM_006711678.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006711678.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCCTGC AGAGCGCGCA GTACCTCCGG CAGGCAGAAG TCCTGAAGGC TGACATGACA 
    GATTCTAAGC TGGGTCCAGC TGAAGTCTGG ACATCCAGGC AGGCTCTGCA GGACCTGTAC
    CAGAAAATGC TAGTTACCGA TTTGGAATAC GCTTTAGACA AGAAAGTAGA ACAGGATCTC
    TGGAATCACG CCTTTAAGAA TCAGATCACA ACACTGCAAG GCCAGGCAAA GAATCGAGCA
    AATCCGAATC GGAGTGAAGT TCAGGCAAAC CTTTCTCTGT TCCTAGAGGC AGCTAGTGGC
    TTCTATACTC AGTTATTACA AGAACTGTGT ACAGTATTTA ATGTAGATTT ACCATGCCGT
    GTGAAGTCTT CCCAATTGGG AATTATCAGC AATAAACAGA CGCATACCAG CGCCATAGTG
    AAGCCACAGT CTAGCTCCTG TTCCTATATC TGCCAGCACT GCCTCGTCCA CCTTGGAGAC
    ATTGCTCGAT ACAGAAACCA GACCAGCCAG GCAGAGTCCT ACTATAGGCA TGCAGCTCAG
    CTTGTCCCCT CCAATGGTCA GCCTTATAAT CAGTTGGCTA TCTTAGCTTC TTCCAAAGGA
    GACCATCTGA CCACAATTTT CTACTACTGC AGAAGCATTG CTGTGAAGTT CCCTTTCCCA
    GCTGCCTCCA CTAATCTGCA AAAAGCACTT TCTAAAGCAC TGGAAAGCCG AGATGAGGTG
    AAAACCAAGT GGGGTGTTTC TGACTTCATC AAGGCCTTTA TTAAATTCCA CGGTCATGTG
    TACCTGAGTA AGAGCTTGGA AAAGTTGAGC CCTCTTCGAG AGAAATTGGA AGAACAGTTT
    AAGAGGCTGC TATTCCAAAA AGCTTTCAAC TCTCAGCAGT TAGTTCATGT CACTGTCATT
    AACCTGTTTC AACTTCATCA CCTTCGTGAC TTTAGCAATG AAACCGAGCA GCACACTTAT
    AGCCAAGATG AGCAGCTATG TTGGACACAG TTGCTGGCCC TCTTTATGTC TTTTCTTGGC
    ATCCTGTGCA AGTGTCCTCT ACAGAATGAG TCTCAGGAGG AGTCCTACAA TGCCTATCCT
    CTTCCAGCAG TCAAGGTCTC CATGGACTGG CTAAGACTCA GACCCAGGGT CTTTCAGGAG
    GCAGTGGTGG ATGAAAGACA GTACATTTGG CCCTGGTTGA TTTCTCTTCT GAATAGTTTC
    CATCCCCATG AAGAGGACCT CTCAAGTATT AGTGCGACAC CACTTCCAGA GGAGTTTGAA
    TTACAAGGAT TTTTGGCATT GAGACCTTCT TTCAGGAACT TGGATTTTTC CAAAGGTCAC
    CAGGGTATTA CAGGGGACAA AGAAGGCCAG CAACGACGAA TACGACAGCA ACGCTTGATC
    TCTATAGGCA AATGGATTGC TGATAATCAG CCAAGGCTGA TTCAGTGTGA AAATGAGGTA
    GGGAAATTGT TGTTTATCAC AGAAATCCCA GAATTAATAC TGGAAGACCC CAGTGAAGCC
    AAAGAGAACC TCATTCTGCA AGAAACATCT GTGATAGAGT CGCTGGCTGC AGATGGGAGC
    CCAGGGCTAA AATCAGTGCT ATCTACAAGC CGAAATTTAA GCAACAACTG TGACACAGGA
    GAGAAGCCAG TGGTTACCTT CAAAGAAAAC ATTAAGACAC GAGAAGTGAA CAGAGACCAA
    GGAAGAAGTT TTCCTCCCAA AGAGGTAAAA TCCCAGACAG AACTAAGAAA GACTCCAGTG
    TCTGAAGCCA GAAAAACACC TGTAACTCAA ACCCCAACTC AAGCAAGTAA CTCCCAGTTC
    ATCCCCATTC ATCACCCTGG AGCCTTCCCT CCTCTTCCCA GCAGGCCAGG GTTTCCGCCC
    CCAACATATG TTATCCCCCC GCCTGTGGCA TTTTCTATGG GCTCAGGTTA CACCTTCCCA
    GCTGGTGTTT CTGTCCCAGG AACCTTTCTT CAGCCTACAG CTCACTCTCC AGCAGGAAAC
    CAGGTGCAAG CTGGGAAACA GTCCCACATT CCTTACAGCC AGCAACGGCC CTCTGGACCA
    GGGCCAATGA ACCAGGGACC TCAACAATCA CAGCCACCTT CCCAGCAACC CCTTACATCT
    TTACCAGCTC AGCCAACAGC ACAGTCTACA AGCCAGCTGC AGGTTCAAGC TCTAACTCAG
    CAACAACAAT CCCCTACAAA AGCTGTGCCG GCTTTGGGGA AAAGCCCGCC TCACCACTCT
    GGATTCCAGC AGTATCAACA GGCAGATGCC TCCAAACAGC TGTGGAATCC CCCTCAGGTT
    CAAGGCCCAT TAGGGAAAAT TATGCCTGTG AAACAGCCCT ACTACCTTCA GACCCAAGAC
    CCCATAAAAC TGTTTGAGCC GTCATTGCAA CCTCCTGTAA TGCAGCAGCA GCCTCTAGAA
    AAAAAAATGA AGCCTTTTCC CATGGAGCCA TATAACCATA ATCCCTCAGA AGTCAAGGTC
    CCAGAATTCT ACTGGGATTC TTCCTACAGC ATGGCTGATA ACAGATCTGT AATGGCACAG
    CAAGCAAACA TAGACCGCAG GGGCAAACGG TCACCAGGAG TCTTCCGTCC AGAGCAGGAT
    CCTGTACCCA GAATGCCGTT TGAGGACCCC AAGAGCTCCC CTCTGCTTCC TCCGGACCTG
    TTAAAGAGTC TGGCTGCCTT GGAGGAAGAG GAAGAGCTGA TTTTTTCTAA CCCTCCTGAT
    CTTTACCCGG CTCTGCTGGG GCCTCTCGCC TCTCTTCCTG GACGAAGCCT TTTTAAATCC
    TTATTGGAGA AGCCCTCAGA GCTCATGTCA CATTCATCCT CTTTCCTGTC CCTCACCGGA
    TTCTCTCTCA ATCAGGAAAG ATACCCAAAT AATAGTATGT TCAATGAGGT ATATGGGAAA
    AACCTGACAT CCAGCTCCAA AGCAGAACTC AGTCCCTCAA TGGCCCCCCA GGAAACATCT
    CTGTATTCCC TTTTTGAAGG GACTCCGTGG TCTCCATCAC TTCCTGCCAG TTCAGATCAT
    TCAACACCAG CCAGCCAGTC TCCTCATTCC TCTAACCCAA GCAGCCTACC CAGCTCTCCT
    CCAACACACA ACCATAATTC TGTTCCATTC TCCAATTTTG GACCCATTGG GACTCCAGAT
    AACAGGGATA GAAGGACTGC AGATCGGTGG AAAACTGATA AGCCAGCCAT GGGTGGGTTT
    GGCATTGATT ATCTCTCAGC AACGTCATCC TCTGAGAGCA GTTGGCATCA GGCCAGCACT
    CCGAGTGGCA CCTGGACAGG CCATGGCCCT TCCATGGAGG ATTCCTCTGC TGTCCTCATG
    GAAAGCCTAA AGTCTATCTG GTCCAGTTCC ATGATGCATC CTGGACCTTC TGCTCTGGAG
    CAGCTGTTAA TGCAGCAGAA GCAGAAACAG CAACGGGGAC AAGGCACCAT GAACCCTCCA
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63454
    Clone ID Related Accession (Same CDS sequence) XM_011510208.2 , XM_011510208.3
    Accession Version XM_011510208.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508510.1
    CDS1027..4527
    Translation

    Target ORF information:

    RefSeq Version XM_011510208.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510208.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63454
    Clone ID Related Accession (Same CDS sequence) XM_011510208.2 , XM_011510208.3
    Accession Version XM_011510208.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508510.1
    CDS996..4496
    Translation

    Target ORF information:

    RefSeq Version XM_011510208.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510208.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTGAGAAGG
    GACTATAGCA AAGGAATAAC TGTAACTAAG AATGATGGAA AGAAGGACAA CAACAAGAGG
    AAAACTGAAA CCAAGAAATG CACCTTAGAA AAGTTACAGG AAACAGGAAA GCAGAATGTG
    GCAGTGCAGG TAAAATCCCA GACAGAACTA AGAAAGACTC CAGTGTCTGA AGCCAGAAAA
    ACACCTGTAA CTCAAACCCC AACTCAAGCA AGTAACTCCC AGTTCATCCC CATTCATCAC
    CCTGGAGCCT TCCCTCCTCT TCCCAGCAGG CCAGGGTTTC CGCCCCCAAC ATATGTTATC
    CCCCCGCCTG TGGCATTTTC TATGGGCTCA GGTTACACCT TCCCAGCTGG TGTTTCTGTC
    CCAGGAACCT TTCTTCAGCC TACAGCTCAC TCTCCAGCAG GAAACCAGGT GCAAGCTGGG
    AAACAGTCCC ACATTCCTTA CAGCCAGCAA CGGCCCTCTG GACCAGGGCC AATGAACCAG
    GGACCTCAAC AATCACAGCC ACCTTCCCAG CAACCCCTTA CATCTTTACC AGCTCAGCCA
    ACAGCACAGT CTACAAGCCA GCTGCAGGTT CAAGCTCTAA CTCAGCAACA ACAATCCCCT
    ACAAAAGCTG TGCCGGCTTT GGGGAAAAGC CCGCCTCACC ACTCTGGATT CCAGCAGTAT
    CAACAGGCAG ATGCCTCCAA ACAGCTGTGG AATCCCCCTC AGGTTCAAGG CCCATTAGGG
    AAAATTATGC CTGTGAAACA GCCCTACTAC CTTCAGACCC AAGACCCCAT AAAACTGTTT
    GAGCCGTCAT TGCAACCTCC TGTAATGCAG CAGCAGCCTC TAGAAAAAAA AATGAAGCCT
    TTTCCCATGG AGCCATATAA CCATAATCCC TCAGAAGTCA AGGTCCCAGA ATTCTACTGG
    GATTCTTCCT ACAGCATGGC TGATAACAGA TCTGTAATGG CACAGCAAGC AAACATAGAC
    CGCAGGGGCA AACGGTCACC AGGAGTCTTC CGTCCAGAGC AGGATCCTGT ACCCAGAATG
    CCGTTTGAGA AATCCTTATT GGAGAAGCCC TCAGAGCTCA TGTCACATTC ATCCTCTTTC
    CTGTCCCTCA CCGGATTCTC TCTCAATCAG GAAAGATACC CAAATAATAG TATGTTCAAT
    GAGGTATATG GGAAAAACCT GACATCCAGC TCCAAAGCAG AACTCAGTCC CTCAATGGCC
    CCCCAGGAAA CATCTCTGTA TTCCCTTTTT GAAGGGACTC CGTGGTCTCC ATCACTTCCT
    GCCAGTTCAG ATCATTCAAC ACCAGCCAGC CAGTCTCCTC ATTCCTCTAA CCCAAGCAGC
    CTACCCAGCT CTCCTCCAAC ACACAACCAT AATTCTGTTC CATTCTCCAA TTTTGGACCC
    ATTGGGACTC CAGATAACAG GGATAGAAGG ACTGCAGATC GGTGGAAAAC TGATAAGCCA
    GCCATGGGTG GGTTTGGCAT TGATTATCTC TCAGCAACGT CATCCTCTGA GAGCAGTTGG
    CATCAGGCCA GCACTCCGAG TGGCACCTGG ACAGGCCATG GCCCTTCCAT GGAGGATTCC
    TCTGCTGTCC TCATGGAAAG CCTAAAGTCT ATCTGGTCCA GTTCCATGAT GCATCCTGGA
    CCTTCTGCTC TGGAGCAGCT GTTAATGCAG CAGAAGCAGA AACAGCAACG GGGACAAGGC
    ACCATGAACC CTCCACACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63455
    Clone ID Related Accession (Same CDS sequence) NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
    Accession Version XM_011510209.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508511.1
    CDS996..4358
    Translation

    Target ORF information:

    RefSeq Version XM_011510209.3
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510209.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63455
    Clone ID Related Accession (Same CDS sequence) NM_001350219.1 , NM_001350219.2 , XM_011510209.3 , XM_011510209.2
    Accession Version XM_011510209.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508511.1
    CDS1027..4389
    Translation

    Target ORF information:

    RefSeq Version XM_011510209.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7, nonsense mediated mRNA decay factor (SMG7), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510209.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGAAATT GGATGTTCTT GCAGATAGTC ATTGTGGGAG AAAACGTTTC ATTTAAGTCC 
    CAGATGAGGA CCGAAAACTT GAAATCAGAG GAGCATCTGA AATCAAGTAA TATTAGGCAG
    GCAGAAGTCC TGAAGGCTGA CATGACAGAT TCTAAGCTGG GTCCAGCTGA AGTCTGGACA
    TCCAGGCAGG CTCTGCAGGA CCTGTACCAG AAAATGCTAG TTACCGATTT GGAATACGCT
    TTAGACAAGA AAGTAGAACA GGATCTCTGG AATCACGCCT TTAAGAATCA GATCACAACA
    CTGCAAGGCC AGGCAAAGAA TCGAGCAAAT CCGAATCGGA GTGAAGTTCA GGCAAACCTT
    TCTCTGTTCC TAGAGGCAGC TAGTGGCTTC TATACTCAGT TATTACAAGA ACTGTGTACA
    GTATTTAATG TAGATTTACC ATGCCGTGTG AAGTCTTCCC AATTGGGAAT TATCAGCAAT
    AAACAGACGC ATACCAGCGC CATAGTGAAG CCACAGTCTA GCTCCTGTTC CTATATCTGC
    CAGCACTGCC TCGTCCACCT TGGAGACATT GCTCGATACA GAAACCAGAC CAGCCAGGCA
    GAGTCCTACT ATAGGCATGC AGCTCAGCTT GTCCCCTCCA ATGGTCAGCC TTATAATCAG
    TTGGCTATCT TAGCTTCTTC CAAAGGAGAC CATCTGACCA CAATTTTCTA CTACTGCAGA
    AGCATTGCTG TGAAGTTCCC TTTCCCAGCT GCCTCCACTA ATCTGCAAAA AGCACTTTCT
    AAAGCACTGG AAAGCCGAGA TGAGGTGAAA ACCAAGTGGG GTGTTTCTGA CTTCATCAAG
    GCCTTTATTA AATTCCACGG TCATGTGTAC CTGAGTAAGA GCTTGGAAAA GTTGAGCCCT
    CTTCGAGAGA AATTGGAAGA ACAGTTTAAG AGGCTGCTAT TCCAAAAAGC TTTCAACTCT
    CAGCAGTTAG TTCATGTCAC TGTCATTAAC CTGTTTCAAC TTCATCACCT TCGTGACTTT
    AGCAATGAAA CCGAGCAGCA CACTTATAGC CAAGATGAGC AGCTATGTTG GACACAGTTG
    CTGGCCCTCT TTATGTCTTT TCTTGGCATC CTGTGCAAGT GTCCTCTACA GAATGAGTCT
    CAGGAGGAGT CCTACAATGC CTATCCTCTT CCAGCAGTCA AGGTCTCCAT GGACTGGCTA
    AGACTCAGAC CCAGGGTCTT TCAGGAGGCA GTGGTGGATG AAAGACAGTA CATTTGGCCC
    TGGTTGATTT CTCTTCTGAA TAGTTTCCAT CCCCATGAAG AGGACCTCTC AAGTATTAGT
    GCGACACCAC TTCCAGAGGA GTTTGAATTA CAAGGATTTT TGGCATTGAG ACCTTCTTTC
    AGGAACTTGG ATTTTTCCAA AGGTCACCAG GGTATTACAG GGGACAAAGA AGGCCAGCAA
    CGACGAATAC GACAGCAACG CTTGATCTCT ATAGGCAAAT GGATTGCTGA TAATCAGCCA
    AGGCTGATTC AGTGTGAAAA TGAGGTAGGG AAATTGTTGT TTATCACAGA AATCCCAGAA
    TTAATACTGG AAGACCCCAG TGAAGCCAAA GAGAACCTCA TTCTGCAAGA AACATCTGTG
    ATAGAGTCGC TGGCTGCAGA TGGGAGCCCA GGGCTAAAAT CAGTGCTATC TACAAGCCGA
    AATTTAAGCA ACAACTGTGA CACAGGAGAG AAGCCAGTGG TTACCTTCAA AGAAAACATT
    AAGACACGAG AAGTGAACAG AGACCAAGGA AGAAGTTTTC CTCCCAAAGA GGTAAAATCC
    CAGACAGAAC TAAGAAAGAC TCCAGTGTCT GAAGCCAGAA AAACACCTGT AACTCAAACC
    CCAACTCAAG CAAGTAACTC CCAGTTCATC CCCATTCATC ACCCTGGAGC CTTCCCTCCT
    CTTCCCAGCA GGCCAGGGTT TCCGCCCCCA ACATATGTTA TCCCCCCGCC TGTGGCATTT
    TCTATGGGCT CAGGTTACAC CTTCCCAGCT GGTGTTTCTG TCCCAGGAAC CTTTCTTCAG
    CCTACAGCTC ACTCTCCAGC AGGAAACCAG GTGCAAGCTG GGAAACAGTC CCACATTCCT
    TACAGCCAGC AACGGCCCTC TGGACCAGGG CCAATGAACC AGGGACCTCA ACAATCACAG
    CCACCTTCCC AGCAACCCCT TACATCTTTA CCAGCTCAGC CAACAGCACA GTCTACAAGC
    CAGCTGCAGG TTCAAGCTCT AACTCAGCAA CAACAATCCC CTACAAAAGC TGTGCCGGCT
    TTGGGGAAAA GCCCGCCTCA CCACTCTGGA TTCCAGCAGT ATCAACAGGC AGATGCCTCC
    AAACAGCTGT GGAATCCCCC TCAGGTTCAA GGCCCATTAG GGAAAATTAT GCCTGTGAAA
    CAGCCCTACT ACCTTCAGAC CCAAGACCCC ATAAAACTGT TTGAGCCGTC ATTGCAACCT
    CCTGTAATGC AGCAGCAGCC TCTAGAAAAA AAAATGAAGC CTTTTCCCAT GGAGCCATAT
    AACCATAATC CCTCAGAAGT CAAGGTCCCA GAATTCTACT GGGATTCTTC CTACAGCATG
    GCTGATAACA GATCTGTAAT GGCACAGCAA GCAAACATAG ACCGCAGGGG CAAACGGTCA
    CCAGGAGTCT TCCGTCCAGA GCAGGATCCT GTACCCAGAA TGCCGTTTGA GAAATCCTTA
    TTGGAGAAGC CCTCAGAGCT CATGTCACAT TCATCCTCTT TCCTGTCCCT CACCGGATTC
    TCTCTCAATC AGGAAAGATA CCCAAATAAT AGTATGTTCA ATGAGGTATA TGGGAAAAAC
    CTGACATCCA GCTCCAAAGC AGAACTCAGT CCCTCAATGG CCCCCCAGGA AACATCTCTG
    TATTCCCTTT TTGAAGGGAC TCCGTGGTCT CCATCACTTC CTGCCAGTTC AGATCATTCA
    ACACCAGCCA GCCAGTCTCC TCATTCCTCT AACCCAAGCA GCCTACCCAG CTCTCCTCCA
    ACACACAACC ATAATTCTGT TCCATTCTCC AATTTTGGAC CCATTGGGAC TCCAGATAAC
    AGGGATAGAA GGACTGCAGA TCGGTGGAAA ACTGATAAGC CAGCCATGGG TGGGTTTGGC
    ATTGATTATC TCTCAGCAAC GTCATCCTCT GAGAGCAGTT GGCATCAGGC CAGCACTCCG
    AGTGGCACCT GGACAGGCCA TGGCCCTTCC ATGGAGGATT CCTCTGCTGT CCTCATGGAA
    AGCCTAAAGT CTATCTGGTC CAGTTCCATG ATGCATCCTG GACCTTCTGC TCTGGAGCAG
    CTGTTAATGC AGCAGAAGCA GAAACAGCAA CGGGGACAAG GCACCATGAA CCCTCCACAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu63456
    Clone ID Related Accession (Same CDS sequence) XM_011510210.2
    Accession Version XM_011510210.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product protein SMG7 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510210.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGGA CCCCAAGAGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG
    AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC
    CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGAA GCCTTTTTAA ATCCTTATTG
    GAGAAGCCCT CAGAGCTCAT GTCACATTCA TCCTCTTTCC TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAATAGT ATGTTCAATG AGGTATATGG GAAAAACCTG
    ACATCCAGCT CCAAAGCAGA ACTCAGTCCC TCAATGGCCC CCCAGGAAAC ATCTCTGTAT
    TCCCTTTTTG AAGGGACTCC GTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TACCCAGCTC TCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAT TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGAAGGA CTGCAGATCG GTGGAAAACT GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GATTATCTCT CAGCAACGTC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTCCGAGT
    GGCACCTGGA CAGGCCATGG CCCTTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTAAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCTGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA ACAGCAACGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508512.1
    CDS216..3515
    Translation

    Target ORF information:

    RefSeq Version XM_011510210.2
    Organism Homo sapiens(human)
    Definition Homo sapiens SMG7 nonsense mediated mRNA decay factor (SMG7), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510210.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTAGTTA CCGATTTGGA ATACGCTTTA GACAAGAAAG TAGAACAGGA TCTCTGGAAT 
    CACGCCTTTA AGAATCAGAT CACAACACTG CAAGGCCAGG CAAAGAATCG AGCAAATCCG
    AATCGGAGTG AAGTTCAGGC AAACCTTTCT CTGTTCCTAG AGGCAGCTAG TGGCTTCTAT
    ACTCAGTTAT TACAAGAACT GTGTACAGTA TTTAATGTAG ATTTACCATG CCGTGTGAAG
    TCTTCCCAAT TGGGAATTAT CAGCAATAAA CAGACGCATA CCAGCGCCAT AGTGAAGCCA
    CAGTCTAGCT CCTGTTCCTA TATCTGCCAG CACTGCCTCG TCCACCTTGG AGACATTGCT
    CGATACAGAA ACCAGACCAG CCAGGCAGAG TCCTACTATA GGCATGCAGC TCAGCTTGTC
    CCCTCCAATG GTCAGCCTTA TAATCAGTTG GCTATCTTAG CTTCTTCCAA AGGAGACCAT
    CTGACCACAA TTTTCTACTA CTGCAGAAGC ATTGCTGTGA AGTTCCCTTT CCCAGCTGCC
    TCCACTAATC TGCAAAAAGC ACTTTCTAAA GCACTGGAAA GCCGAGATGA GGTGAAAACC
    AAGTGGGGTG TTTCTGACTT CATCAAGGCC TTTATTAAAT TCCACGGTCA TGTGTACCTG
    AGTAAGAGCT TGGAAAAGTT GAGCCCTCTT CGAGAGAAAT TGGAAGAACA GTTTAAGAGG
    CTGCTATTCC AAAAAGCTTT CAACTCTCAG CAGTTAGTTC ATGTCACTGT CATTAACCTG
    TTTCAACTTC ATCACCTTCG TGACTTTAGC AATGAAACCG AGCAGCACAC TTATAGCCAA
    GATGAGCAGC TATGTTGGAC ACAGTTGCTG GCCCTCTTTA TGTCTTTTCT TGGCATCCTG
    TGCAAGTGTC CTCTACAGAA TGAGTCTCAG GAGGAGTCCT ACAATGCCTA TCCTCTTCCA
    GCAGTCAAGG TCTCCATGGA CTGGCTAAGA CTCAGACCCA GGGTCTTTCA GGAGGCAGTG
    GTGGATGAAA GACAGTACAT TTGGCCCTGG TTGATTTCTC TTCTGAATAG TTTCCATCCC
    CATGAAGAGG ACCTCTCAAG TATTAGTGCG ACACCACTTC CAGAGGAGTT TGAATTACAA
    GGATTTTTGG CATTGAGACC TTCTTTCAGG AACTTGGATT TTTCCAAAGG TCACCAGGGT
    ATTACAGGGG ACAAAGAAGG CCAGCAACGA CGAATACGAC AGCAACGCTT GATCTCTATA
    GGCAAATGGA TTGCTGATAA TCAGCCAAGG CTGATTCAGT GTGAAAATGA GGTAGGGAAA
    TTGTTGTTTA TCACAGAAAT CCCAGAATTA ATACTGGAAG ACCCCAGTGA AGCCAAAGAG
    AACCTCATTC TGCAAGAAAC ATCTGTGATA GAGTCGCTGG CTGCAGATGG GAGCCCAGGG
    CTAAAATCAG TGCTATCTAC AAGCCGAAAT TTAAGCAACA ACTGTGACAC AGGAGAGAAG
    CCAGTGGTTA CCTTCAAAGA AAACATTAAG ACACGAGAAG TGAACAGAGA CCAAGGAAGA
    AGTTTTCCTC CCAAAGAGGT AAAATCCCAG ACAGAACTAA GAAAGACTCC AGTGTCTGAA
    GCCAGAAAAA CACCTGTAAC TCAAACCCCA ACTCAAGCAA GTAACTCCCA GTTCATCCCC
    ATTCATCACC CTGGAGCCTT CCCTCCTCTT CCCAGCAGGC CAGGGTTTCC GCCCCCAACA
    TATGTTATCC CCCCGCCTGT GGCATTTTCT ATGGGCTCAG GTTACACCTT CCCAGCTGGT
    GTTTCTGTCC CAGGAACCTT TCTTCAGCCT ACAGCTCACT CTCCAGCAGG AAACCAGGTG
    CAAGCTGGGA AACAGTCCCA CATTCCTTAC AGCCAGCAAC GGCCCTCTGG ACCAGGGCCA
    ATGAACCAGG GACCTCAACA ATCACAGCCA CCTTCCCAGC AACCCCTTAC ATCTTTACCA
    GCTCAGCCAA CAGCACAGTC TACAAGCCAG CTGCAGGTTC AAGCTCTAAC TCAGCAACAA
    CAATCCCCTA CAAAAGCTGT GCCGGCTTTG GGGAAAAGCC CGCCTCACCA CTCTGGATTC
    CAGCAGTATC AACAGGCAGA TGCCTCCAAA CAGCTGTGGA ATCCCCCTCA GGTTCAAGGC
    CCATTAGGGA AAATTATGCC TGTGAAACAG CCCTACTACC TTCAGACCCA AGACCCCATA
    AAACTGTTTG AGCCGTCATT GCAACCTCCT GTAATGCAGC AGCAGCCTCT AGAAAAAAAA
    ATGAAGCCTT TTCCCATGGA GCCATATAAC CATAATCCCT CAGAAGTCAA GGTCCCAGAA
    TTCTACTGGG ATTCTTCCTA CAGCATGGCT GATAACAGAT CTGTAATGGC ACAGCAAGCA
    AACATAGACC GCAGGGGCAA ACGGTCACCA GGAGTCTTCC GTCCAGAGCA GGATCCTGTA
    CCCAGAATGC CGTTTGAGGA CCCCAAGAGC TCCCCTCTGC TTCCTCCGGA CCTGTTAAAG
    AGTCTGGCTG CCTTGGAGGA AGAGGAAGAG CTGATTTTTT CTAACCCTCC TGATCTTTAC
    CCGGCTCTGC TGGGGCCTCT CGCCTCTCTT CCTGGACGAA GCCTTTTTAA ATCCTTATTG
    GAGAAGCCCT CAGAGCTCAT GTCACATTCA TCCTCTTTCC TGTCCCTCAC CGGATTCTCT
    CTCAATCAGG AAAGATACCC AAATAATAGT ATGTTCAATG AGGTATATGG GAAAAACCTG
    ACATCCAGCT CCAAAGCAGA ACTCAGTCCC TCAATGGCCC CCCAGGAAAC ATCTCTGTAT
    TCCCTTTTTG AAGGGACTCC GTGGTCTCCA TCACTTCCTG CCAGTTCAGA TCATTCAACA
    CCAGCCAGCC AGTCTCCTCA TTCCTCTAAC CCAAGCAGCC TACCCAGCTC TCCTCCAACA
    CACAACCATA ATTCTGTTCC ATTCTCCAAT TTTGGACCCA TTGGGACTCC AGATAACAGG
    GATAGAAGGA CTGCAGATCG GTGGAAAACT GATAAGCCAG CCATGGGTGG GTTTGGCATT
    GATTATCTCT CAGCAACGTC ATCCTCTGAG AGCAGTTGGC ATCAGGCCAG CACTCCGAGT
    GGCACCTGGA CAGGCCATGG CCCTTCCATG GAGGATTCCT CTGCTGTCCT CATGGAAAGC
    CTAAAGTCTA TCTGGTCCAG TTCCATGATG CATCCTGGAC CTTCTGCTCT GGAGCAGCTG
    TTAATGCAGC AGAAGCAGAA ACAGCAACGG GGACAAGGCA CCATGAACCC TCCACACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.