TCAF1 cDNA ORF clone, Homo sapiens(human)

The following TCAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu01872 NM_001206938.1
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu00303 NM_001206941.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu10892 NM_014719.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $650.30
$929.00
OHu41886 NM_014719.3
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 NM_001206938.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250074.4
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250077.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_006716191.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250075.3
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250076.4
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_017012834.1
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_017012835.1
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_017012836.1
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_006716192.4
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_017012833.2
Latest version!
Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
View Old Accession Versions >>
OHu41886 XM_005250074.3 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250075.2 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41887 XM_017012833.1 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu00303 NM_001206941.1 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu41887 XM_006716192.3 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu41886 XM_005250076.3 Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu01872
    Clone ID Related Accession (Same CDS sequence) NM_001206938.1
    Accession Version NM_001206938.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA317873.1, BC000609.2, AC099548.6 and BU947884.1. Transcript Variant: This variant (2) uses a different splice site in the 3' coding region, which results in the use of an early stop codon, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.19550.1, SRR1803617.58429.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    ATTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193867.1
    CDS174..2933
    Translation

    Target ORF information:

    RefSeq Version NM_001206938.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    ATTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00303
    Clone ID Related Accession (Same CDS sequence) NM_001206941.1 , NM_001206941.2
    Accession Version NM_001206941.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA196926.1, AK299911.1, AB018281.1, AC099548.6 and BU947884.1. Transcript Variant: This variant (3) has several differences in its 5' UTR, one of which results in the use of a downstream in-frame start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK299911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCAGGA AGAGAGGAAA TGTGGAAAAG GGCTGGTTGG CAAAGCTGGG ACCAGATGGT 
    GCAGCTTTCC TGCAGATTCC CGCAGAAGAG ATCCCTGCCT ACATGTCTGT GCATCGACTC
    CTGAGGAAGC TGCTAAGTCG ATATCGGCTT CCAGTAGCAA CCCGAGAGAA CCCTGTTATC
    AATGACTGCT GCAGAGGTGC TATGCTTTCC CTGGCCACAG GGCTGGCCCA CTCTGGAAGC
    GACCTCTCTC TGTTAGTCCC AGAAATTGAA GATATGTACA GCAGCCCCTA TCTGCGCCCC
    TCAGAATCTC CTATCACCGT CGAGGTCAAC TGCACCAATC CAGGCACCAG ATATTGCTGG
    ATGAGTACTG GGCTCTACAT ACCTGGAAGG CAAATTATAG AAGTCTCACT GCCTGAAGCT
    GCTGCCTCTG CCGACCTGAA GATACAGATT GGCTGCCACA CAGATGACCT GACCAGGGCC
    AGCAAGCTTT TCCGAGGCCC ACTCGTAATT AACCGGTGCT GCTTGGACAA ACCCACAAAA
    TCGATCACGT GCCTCTGGGG TGGACTCCTC TATATAATTG TGCCTCAGAA CAGCAAACTG
    GGTTCTGTGC CTGTCACCGT GAAGGGGGCT GTGCATGCTC CATACTACAA GCTGGGGGAG
    ACCACCCTGG AGGAGTGGAA GAGGCGTATC CAGGAGAATC CAGGGCCCTG GGGAGAGCTG
    GCCACGGACA ACATCATTCT GACCGTGCCG ACCGCAAATC TTCGTACTCT GGAGAACCCT
    GAGCCGCTGC TCCGCCTCTG GGATGAGGTG ATGCAGGCTG TGGCGCGACT GGGAGCTGAG
    CCCTTCCCTT TGCGCCTGCC TCAGAGGATT GTTGCCGACG TGCAGATCTC AGTGGGCTGG
    ATGCATGCAG GGTACCCCAT CATGTGCCAT CTGGAGTCAG TGCAGGAGCT CATCAACGAG
    AAGCTCATCA GAACCAAGGG GCTGTGGGGC CCCGTCCATG AGCTGGGCCG CAACCAGCAG
    CGGCAGGAGT GGGAGTTCCC ACCACACACC ACCGAGGCCA CCTGCAACCT GTGGTGTGTG
    TATGTGCATG AGACGGTCTT GGGCATTCCT CGAAGCCGTG CCAATATTGC TCTGTGGCCC
    CCAGTTCGGG AGAAGAGAGT CAGAATCTAC CTGAGCAAGG GTCCCAATGT GAAAAACTGG
    AATGCATGGA CCGCACTGGA AACGTATTTA CAGCTCCAGG AAGCCTTTGG TTGGGAGCCA
    TTCATCCGTC TCTTCACCGA GTACAGGAAC CAGACCAACT TGCCCACAGA AAATGTTGAC
    AAAATGAATC TGTGGGTCAA GATGTTCTCC CACCAAGTGC AGAAGAACCT GGCTCCGTTC
    TTTGAGGCCT GGGCCTGGCC CATCCAGAAG GAAGTGGCTA CCAGCCTGGC CTATCTGCCT
    GAATGGAAGG AAAATATTAT GAAATTGTAC CTCCTCACAC AGATGCCCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193870.1
    CDS817..2310
    Translation

    Target ORF information:

    RefSeq Version NM_001206941.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCAGGA AGAGAGGAAA TGTGGAAAAG GGCTGGTTGG CAAAGCTGGG ACCAGATGGT 
    GCAGCTTTCC TGCAGATTCC CGCAGAAGAG ATCCCTGCCT ACATGTCTGT GCATCGACTC
    CTGAGGAAGC TGCTAAGTCG ATATCGGCTT CCAGTAGCAA CCCGAGAGAA CCCTGTTATC
    AATGACTGCT GCAGAGGTGC TATGCTTTCC CTGGCCACAG GGCTGGCCCA CTCTGGAAGC
    GACCTCTCTC TGTTAGTCCC AGAAATTGAA GATATGTACA GCAGCCCCTA TCTGCGCCCC
    TCAGAATCTC CTATCACCGT CGAGGTCAAC TGCACCAATC CAGGCACCAG ATATTGCTGG
    ATGAGTACTG GGCTCTACAT ACCTGGAAGG CAAATTATAG AAGTCTCACT GCCTGAAGCT
    GCTGCCTCTG CCGACCTGAA GATACAGATT GGCTGCCACA CAGATGACCT GACCAGGGCC
    AGCAAGCTTT TCCGAGGCCC ACTCGTAATT AACCGGTGCT GCTTGGACAA ACCCACAAAA
    TCGATCACGT GCCTCTGGGG TGGACTCCTC TATATAATTG TGCCTCAGAA CAGCAAACTG
    GGTTCTGTGC CTGTCACCGT GAAGGGGGCT GTGCATGCTC CATACTACAA GCTGGGGGAG
    ACCACCCTGG AGGAGTGGAA GAGGCGTATC CAGGAGAATC CAGGGCCCTG GGGAGAGCTG
    GCCACGGACA ACATCATTCT GACCGTGCCG ACCGCAAATC TTCGTACTCT GGAGAACCCT
    GAGCCGCTGC TCCGCCTCTG GGATGAGGTG ATGCAGGCTG TGGCGCGACT GGGAGCTGAG
    CCCTTCCCTT TGCGCCTGCC TCAGAGGATT GTTGCCGACG TGCAGATCTC AGTGGGCTGG
    ATGCATGCAG GGTACCCCAT CATGTGCCAT CTGGAGTCAG TGCAGGAGCT CATCAACGAG
    AAGCTCATCA GAACCAAGGG GCTGTGGGGC CCCGTCCATG AGCTGGGCCG CAACCAGCAG
    CGGCAGGAGT GGGAGTTCCC ACCACACACC ACCGAGGCCA CCTGCAACCT GTGGTGTGTG
    TATGTGCATG AGACGGTCTT GGGCATTCCT CGAAGCCGTG CCAATATTGC TCTGTGGCCC
    CCAGTTCGGG AGAAGAGAGT CAGAATCTAC CTGAGCAAGG GTCCCAATGT GAAAAACTGG
    AATGCATGGA CCGCACTGGA AACGTATTTA CAGCTCCAGG AAGCCTTTGG TTGGGAGCCA
    TTCATCCGTC TCTTCACCGA GTACAGGAAC CAGACCAACT TGCCCACAGA AAATGTTGAC
    AAAATGAATC TGTGGGTCAA GATGTTCTCC CACCAAGTGC AGAAGAACCT GGCTCCGTTC
    TTTGAGGCCT GGGCCTGGCC CATCCAGAAG GAAGTGGCTA CCAGCCTGGC CTATCTGCCT
    GAATGGAAGG AAAATATTAT GAAATTGTAC CTCCTCACAC AGATGCCCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00303
    Clone ID Related Accession (Same CDS sequence) NM_001206941.1 , NM_001206941.2
    Accession Version NM_001206941.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004742.1 and AC099548.6. On May 31, 2019 this sequence version replaced NM_001206941.1. Transcript Variant: This variant (3) has several differences in its 5' UTR, one of which results in the use of a downstream in-frame start codon, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK299911.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCAGGA AGAGAGGAAA TGTGGAAAAG GGCTGGTTGG CAAAGCTGGG ACCAGATGGT 
    GCAGCTTTCC TGCAGATTCC CGCAGAAGAG ATCCCTGCCT ACATGTCTGT GCATCGACTC
    CTGAGGAAGC TGCTAAGTCG ATATCGGCTT CCAGTAGCAA CCCGAGAGAA CCCTGTTATC
    AATGACTGCT GCAGAGGTGC TATGCTTTCC CTGGCCACAG GGCTGGCCCA CTCTGGAAGC
    GACCTCTCTC TGTTAGTCCC AGAAATTGAA GATATGTACA GCAGCCCCTA TCTGCGCCCC
    TCAGAATCTC CTATCACCGT CGAGGTCAAC TGCACCAATC CAGGCACCAG ATATTGCTGG
    ATGAGTACTG GGCTCTACAT ACCTGGAAGG CAAATTATAG AAGTCTCACT GCCTGAAGCT
    GCTGCCTCTG CCGACCTGAA GATACAGATT GGCTGCCACA CAGATGACCT GACCAGGGCC
    AGCAAGCTTT TCCGAGGCCC ACTCGTAATT AACCGGTGCT GCTTGGACAA ACCCACAAAA
    TCGATCACGT GCCTCTGGGG TGGACTCCTC TATATAATTG TGCCTCAGAA CAGCAAACTG
    GGTTCTGTGC CTGTCACCGT GAAGGGGGCT GTGCATGCTC CATACTACAA GCTGGGGGAG
    ACCACCCTGG AGGAGTGGAA GAGGCGTATC CAGGAGAATC CAGGGCCCTG GGGAGAGCTG
    GCCACGGACA ACATCATTCT GACCGTGCCG ACCGCAAATC TTCGTACTCT GGAGAACCCT
    GAGCCGCTGC TCCGCCTCTG GGATGAGGTG ATGCAGGCTG TGGCGCGACT GGGAGCTGAG
    CCCTTCCCTT TGCGCCTGCC TCAGAGGATT GTTGCCGACG TGCAGATCTC AGTGGGCTGG
    ATGCATGCAG GGTACCCCAT CATGTGCCAT CTGGAGTCAG TGCAGGAGCT CATCAACGAG
    AAGCTCATCA GAACCAAGGG GCTGTGGGGC CCCGTCCATG AGCTGGGCCG CAACCAGCAG
    CGGCAGGAGT GGGAGTTCCC ACCACACACC ACCGAGGCCA CCTGCAACCT GTGGTGTGTG
    TATGTGCATG AGACGGTCTT GGGCATTCCT CGAAGCCGTG CCAATATTGC TCTGTGGCCC
    CCAGTTCGGG AGAAGAGAGT CAGAATCTAC CTGAGCAAGG GTCCCAATGT GAAAAACTGG
    AATGCATGGA CCGCACTGGA AACGTATTTA CAGCTCCAGG AAGCCTTTGG TTGGGAGCCA
    TTCATCCGTC TCTTCACCGA GTACAGGAAC CAGACCAACT TGCCCACAGA AAATGTTGAC
    AAAATGAATC TGTGGGTCAA GATGTTCTCC CACCAAGTGC AGAAGAACCT GGCTCCGTTC
    TTTGAGGCCT GGGCCTGGCC CATCCAGAAG GAAGTGGCTA CCAGCCTGGC CTATCTGCCT
    GAATGGAAGG AAAATATTAT GAAATTGTAC CTCCTCACAC AGATGCCCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193870.1
    CDS814..2307
    Translation

    Target ORF information:

    RefSeq Version NM_001206941.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206941.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGGCAGGA AGAGAGGAAA TGTGGAAAAG GGCTGGTTGG CAAAGCTGGG ACCAGATGGT 
    GCAGCTTTCC TGCAGATTCC CGCAGAAGAG ATCCCTGCCT ACATGTCTGT GCATCGACTC
    CTGAGGAAGC TGCTAAGTCG ATATCGGCTT CCAGTAGCAA CCCGAGAGAA CCCTGTTATC
    AATGACTGCT GCAGAGGTGC TATGCTTTCC CTGGCCACAG GGCTGGCCCA CTCTGGAAGC
    GACCTCTCTC TGTTAGTCCC AGAAATTGAA GATATGTACA GCAGCCCCTA TCTGCGCCCC
    TCAGAATCTC CTATCACCGT CGAGGTCAAC TGCACCAATC CAGGCACCAG ATATTGCTGG
    ATGAGTACTG GGCTCTACAT ACCTGGAAGG CAAATTATAG AAGTCTCACT GCCTGAAGCT
    GCTGCCTCTG CCGACCTGAA GATACAGATT GGCTGCCACA CAGATGACCT GACCAGGGCC
    AGCAAGCTTT TCCGAGGCCC ACTCGTAATT AACCGGTGCT GCTTGGACAA ACCCACAAAA
    TCGATCACGT GCCTCTGGGG TGGACTCCTC TATATAATTG TGCCTCAGAA CAGCAAACTG
    GGTTCTGTGC CTGTCACCGT GAAGGGGGCT GTGCATGCTC CATACTACAA GCTGGGGGAG
    ACCACCCTGG AGGAGTGGAA GAGGCGTATC CAGGAGAATC CAGGGCCCTG GGGAGAGCTG
    GCCACGGACA ACATCATTCT GACCGTGCCG ACCGCAAATC TTCGTACTCT GGAGAACCCT
    GAGCCGCTGC TCCGCCTCTG GGATGAGGTG ATGCAGGCTG TGGCGCGACT GGGAGCTGAG
    CCCTTCCCTT TGCGCCTGCC TCAGAGGATT GTTGCCGACG TGCAGATCTC AGTGGGCTGG
    ATGCATGCAG GGTACCCCAT CATGTGCCAT CTGGAGTCAG TGCAGGAGCT CATCAACGAG
    AAGCTCATCA GAACCAAGGG GCTGTGGGGC CCCGTCCATG AGCTGGGCCG CAACCAGCAG
    CGGCAGGAGT GGGAGTTCCC ACCACACACC ACCGAGGCCA CCTGCAACCT GTGGTGTGTG
    TATGTGCATG AGACGGTCTT GGGCATTCCT CGAAGCCGTG CCAATATTGC TCTGTGGCCC
    CCAGTTCGGG AGAAGAGAGT CAGAATCTAC CTGAGCAAGG GTCCCAATGT GAAAAACTGG
    AATGCATGGA CCGCACTGGA AACGTATTTA CAGCTCCAGG AAGCCTTTGG TTGGGAGCCA
    TTCATCCGTC TCTTCACCGA GTACAGGAAC CAGACCAACT TGCCCACAGA AAATGTTGAC
    AAAATGAATC TGTGGGTCAA GATGTTCTCC CACCAAGTGC AGAAGAACCT GGCTCCGTTC
    TTTGAGGCCT GGGCCTGGCC CATCCAGAAG GAAGTGGCTA CCAGCCTGGC CTATCTGCCT
    GAATGGAAGG AAAATATTAT GAAATTGTAC CTCCTCACAC AGATGCCCCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10892
    Clone ID Related Accession (Same CDS sequence) NM_014719.2
    Accession Version NM_014719.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB018281.1, AC099548.6 and BU947884.1. On May 11, 2011 this sequence version replaced NM_014719.1. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly except for one retained mismatch in the coding region. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB018281.1, SRR1660807.102828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    ATTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055534.1
    CDS134..2899
    Translation

    Target ORF information:

    RefSeq Version NM_014719.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    ATTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version NM_014719.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004742.1 and AC099548.6. On Nov 23, 2018 this sequence version replaced NM_014719.2. Transcript Variant: This variant (1) encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly except for one retained mismatch in the coding region. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.102828.1, SRR1660809.119335.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000479870.6/ ENSP00000419235.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055534.2
    CDS231..2996
    Translation

    Target ORF information:

    RefSeq Version NM_014719.3
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014719.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version NM_001206938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004742.1 and AC099548.6. On May 31, 2019 this sequence version replaced NM_001206938.1. Transcript Variant: This variant (2) uses a different splice site in the 3' coding region, which results in the use of an early stop codon, compared to variant 1. The resulting protein (isoform 2) has a shorter C-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.58429.1, SRR7410571.152938.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193867.2
    CDS165..2924
    Translation

    Target ORF information:

    RefSeq Version NM_001206938.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_006716191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006716191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006716254.1
    CDS140..2905
    Translation

    Target ORF information:

    RefSeq Version XM_006716191.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250074.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250131.1
    CDS174..2939
    Translation

    Target ORF information:

    RefSeq Version XM_005250074.3
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250074.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250076.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005250076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250133.1
    CDS211..2976
    Translation

    Target ORF information:

    RefSeq Version XM_005250076.3
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250076.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250074.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005250074.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250131.1
    CDS156..2921
    Translation

    Target ORF information:

    RefSeq Version XM_005250074.4
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250074.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250075.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005250075.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250132.1
    CDS170..2935
    Translation

    Target ORF information:

    RefSeq Version XM_005250075.3
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250075.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250075.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005250075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250132.1
    CDS176..2941
    Translation

    Target ORF information:

    RefSeq Version XM_005250075.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250075.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250076.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005250076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250133.1
    CDS150..2915
    Translation

    Target ORF information:

    RefSeq Version XM_005250076.4
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250076.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41886
    Clone ID Related Accession (Same CDS sequence) XM_006716191.2 , XM_005250074.3 , XM_005250076.3 , XM_005250074.4 , XM_005250075.3 , XM_005250075.2 , NM_014719.3 , XM_005250076.4 , XM_005250077.2
    Accession Version XM_005250077.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005250077.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005250134.1
    CDS340..3105
    Translation

    Target ORF information:

    RefSeq Version XM_005250077.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005250077.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGCCC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_017012835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868324.1
    CDS340..3099
    Translation

    Target ORF information:

    RefSeq Version XM_017012835.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_017012833.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868322.1
    CDS150..2909
    Translation

    Target ORF information:

    RefSeq Version XM_017012833.2
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012833.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_017012836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868325.1
    CDS140..2899
    Translation

    Target ORF information:

    RefSeq Version XM_017012836.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_017012834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868323.1
    CDS176..2935
    Translation

    Target ORF information:

    RefSeq Version XM_017012834.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_017012833.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016868322.1
    CDS206..2965
    Translation

    Target ORF information:

    RefSeq Version XM_017012833.1
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017012833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_006716192.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006716192.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006716255.1
    CDS221..2980
    Translation

    Target ORF information:

    RefSeq Version XM_006716192.4
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716192.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu41887
    Clone ID Related Accession (Same CDS sequence) XM_017012835.1 , NM_001206938.2 , XM_017012833.2 , XM_017012836.1 , XM_017012834.1 , XM_017012833.1 , XM_006716192.4 , XM_006716192.3
    Accession Version XM_006716192.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product TRPM8 channel-associated factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006716255.1
    CDS283..3042
    Translation

    Target ORF information:

    RefSeq Version XM_006716192.3
    Organism Homo sapiens(human)
    Definition Homo sapiens TRPM8 channel associated factor 1 (TCAF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGACTC CCTCTGCTGC CTTCGAGGCC CTTATGAATG GTGTGACAAG CTGGGATGTA 
    CCCGAAGATG CTGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCATT TCCTGTGATG
    GTGAATGACA TGGGCCAGGT CCTCATTGCT GCCTCCTCCT ATGGCCGTGG CCGCCTGGTG
    GTCGTGTCCC ATGAGGACTA CTTGGTGGAA GCCCAGCTCA CGCCCTTTCT CCTGAACGCA
    GTGGGGTGGC TTTGCTCTTC CCCTGGGGCT CCCATTGGTG TACACCCATC CCTGGCACCT
    TTGGCCAAAA TCCTCGAGGG CTCTGGAGTG GATGCAAAGG TTGAGCCAGA AGTGAAAGAC
    TCCCTGGGGG TTTACTGTAT TGATGCCTAC AATGAAACCA TGACAGAAAA GCTGGTCAAG
    TTCATGAAAT GTGGTGGCGG CTTGCTCATA GGAGGACAAG CCTGGGATTG GGCCAACCAG
    GGGGAGGATG AAAGGGTTCT GTTCACGTTC CCTGGGAACC TCGTGACCAG TGTGGCTGGC
    ATTTACTTTA CTGACAACAA AGGGGACACG AGTTTCTTTA AAGTCTCCAA GAAGATGCCC
    AAGATCCCAG TGTTGGTTAG TTGTGAAGAT GACCTCTCCG ACGACAGAGA AGAGCTTCTG
    CATGGGATTT CAGAGCTGGA CATCAGCAAC TCGGATTGTT TCCCATCCCA GCTGCTAGTG
    CATGGGGCTT TAGCCTTTCC TCTAGGGTTA GATTCCTACC ATGGCTGTGT TATAGCGGCT
    GCCCGCTATG GCCGGGGCCG GGTGGTTGTG ACTGGCCATA AGGTATTATT CACTGTTGGT
    AAACTGGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTGG ATGGGGGCCG CAGAGGCAAG
    GTTGTGGTGC AGACAGAGCT GAGAACCCTG AGTGGCCTCC TCGCAGTGGG GGGCATAGAC
    ACCAGCATCG AGCCCAATCT GACCAGTGAT GCAAGTGTCT ACTGCTTTGA ACCCGTGAGT
    GAAGTGGGGG TCAAGGAACT GCAGGAGTTT GTAGCAGAGG GTGGCGGGCT ATTTGTTGGA
    GCCCAAGCCT GGTGGTGGGC CTTCAAGAAC CCCGGAGTGT CCCCTTTGGC TCGATTCCCA
    GGAAACCTCC TCCTCAACCC CTTTGGCATC AGCATTACAA GCCAAAGCCT CAATCCAGGG
    CCCTTTCGTA CTCCTAAAGC AGGGATAAGG ACCTATCACT TCCGCTCCAC CTTGGCCGAG
    TTCCAGGTTA TAATGGGCAG GAAGAGAGGA AATGTGGAAA AGGGCTGGTT GGCAAAGCTG
    GGACCAGATG GTGCAGCTTT CCTGCAGATT CCCGCAGAAG AGATCCCTGC CTACATGTCT
    GTGCATCGAC TCCTGAGGAA GCTGCTAAGT CGATATCGGC TTCCAGTAGC AACCCGAGAG
    AACCCTGTTA TCAATGACTG CTGCAGAGGT GCTATGCTTT CCCTGGCCAC AGGGCTGGCC
    CACTCTGGAA GCGACCTCTC TCTGTTAGTC CCAGAAATTG AAGATATGTA CAGCAGCCCC
    TATCTGCGCC CCTCAGAATC TCCTATCACC GTCGAGGTCA ACTGCACCAA TCCAGGCACC
    AGATATTGCT GGATGAGTAC TGGGCTCTAC ATACCTGGAA GGCAAATTAT AGAAGTCTCA
    CTGCCTGAAG CTGCTGCCTC TGCCGACCTG AAGATACAGA TTGGCTGCCA CACAGATGAC
    CTGACCAGGG CCAGCAAGCT TTTCCGAGGC CCACTCGTAA TTAACCGGTG CTGCTTGGAC
    AAACCCACAA AATCGATCAC GTGCCTCTGG GGTGGACTCC TCTATATAAT TGTGCCTCAG
    AACAGCAAAC TGGGTTCTGT GCCTGTCACC GTGAAGGGGG CTGTGCATGC TCCATACTAC
    AAGCTGGGGG AGACCACCCT GGAGGAGTGG AAGAGGCGTA TCCAGGAGAA TCCAGGGCCC
    TGGGGAGAGC TGGCCACGGA CAACATCATT CTGACCGTGC CGACCGCAAA TCTTCGTACT
    CTGGAGAACC CTGAGCCGCT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCGCGA
    CTGGGAGCTG AGCCCTTCCC TTTGCGCCTG CCTCAGAGGA TTGTTGCCGA CGTGCAGATC
    TCAGTGGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG
    CTCATCAACG AGAAGCTCAT CAGAACCAAG GGGCTGTGGG GCCCCGTCCA TGAGCTGGGC
    CGCAACCAGC AGCGGCAGGA GTGGGAGTTC CCACCACACA CCACCGAGGC CACCTGCAAC
    CTGTGGTGTG TGTATGTGCA TGAGACGGTC TTGGGCATTC CTCGAAGCCG TGCCAATATT
    GCTCTGTGGC CCCCAGTTCG GGAGAAGAGA GTCAGAATCT ACCTGAGCAA GGGTCCCAAT
    GTGAAAAACT GGAATGCATG GACCGCACTG GAAACGTATT TACAGCTCCA GGAAGCCTTT
    GGTTGGGAGC CATTCATCCG TCTCTTCACC GAGTACAGGA ACCAGACCAA CTTGCCCACA
    GAAAATGTTG ACAAAATGAA TCTGTGGGTC AAGATGTTCT CCCACCAAGT GCAGAAGAAC
    CTGGCTCCGT TCTTTGAGGC CTGGGCCTGG CCCATCCAGA AGGAAGTGGC TACCAGCCTG
    GCCTATCTGC CTGAATGGAA GGAAAATATT ATGAAATTGT ACCTCCTCAC ACAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.