RUBCN gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006713829.3	XP_006713892.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4
XM_017007545.1	XP_016863034.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7
XM_006713828.3	XP_006713891.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X2
NM_001145642.4	NP_001139114.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
XM_005269374.3	XP_005269431.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X3
XM_006713831.3	XP_006713894.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
XM_006713829.4	XP_006713892.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4
XM_006713831.4	XP_006713894.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
XM_017007545.2	XP_016863034.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7
NM_001346873.1	NP_001333802.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 3
NM_014687.4	NP_055502.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2
XM_006713827.3	XP_006713890.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X1
XM_017007543.1	XP_016863032.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6
NM_014687.3	NP_055502.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2
XM_006713830.3	XP_006713893.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
XM_024453838.1	XP_024309606.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6
XM_017007544.1	XP_016863033.1	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27911	NM_001145642.4 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu27916	NM_014687.4 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38258	XM_006713828.3 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38259	XM_005269374.3 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38260	XM_006713829.4 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38261	XM_006713831.4 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38261	XM_006713830.3 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu94800	XM_024453838.1 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94800	XM_017007543.1 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94801	XM_017007545.2 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94801	XM_017007544.1 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100758	NM_001346873.1 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu38257	XM_006713827.3 Latest version!	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu38260	XM_006713829.3	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu38261	XM_006713831.3	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu94801	XM_017007545.1	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu27916	NM_014687.3	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

RUBCN ( NM_001145642.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139114.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27911
Clone ID Related Accession (Same CDS sequence)	NM_001145642.4
Accession Version	NM_001145642.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB036516.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2016 this sequence version replaced NM_001145642.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86979.3, SRR1803613.27601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA 
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT 
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC 
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT 
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT 
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT 
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC 
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC 
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG 
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG 
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG 
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT 
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG 
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC 
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA 
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG 
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG 
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG 
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA 
GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139114.1
CDS	547..3330
Translation	MQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	NM_001145642.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145642.4

ORF Insert Sequence:

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA 
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT 
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC 
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT 
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT 
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT 
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC 
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC 
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG 
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG 
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG 
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT 
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG 
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC 
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA 
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG 
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG 
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG 
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA 
GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( NM_014687.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055502.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27916
Clone ID Related Accession (Same CDS sequence)	NM_014687.4 , NM_014687.3
Accession Version	NM_014687.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY003277.1, BC033615.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_014687.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296343.10/ ENSP00000296343.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055502.1
CDS	186..3104
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	NM_014687.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014687.4

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( NM_014687.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055502.1 Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant 2, mRNA.

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CloneID	OHu27916
Clone ID Related Accession (Same CDS sequence)	NM_014687.4 , NM_014687.3
Accession Version	NM_014687.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY003277.1, BC033615.1, D86979.3, AC024560.26 and AI796534.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2016 this sequence version replaced NM_014687.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055502.1
CDS	208..3126
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	NM_014687.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014687.3

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713828.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713891.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X2, mRNA.

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CloneID	OHu38258
Clone ID Related Accession (Same CDS sequence)	XM_006713828.3
Accession Version	XM_006713828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713828.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGG TAGCGAAGGA 
TCTAACCTGA CTCACATATC AAAGAACGGA CTGTCAGTGT CACTGGCCTC GATGTTCTCA 
GATGCTGACA TCAGAAGGAA CACAGCCTCA AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC 
TTCTCCCACT GCTTCCTGCA CTCCACGTCT GCTGAGGCGG TGGCCATGGG GCTCCTGAAG 
CAGTTTGAGG GGATGCAGCT TCCAGCCGCC TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT 
GATGCCCCTC AGAAGCTCCT GCCCATTCCT GACTCACTGC CCATCTCACC GGATGACGGG 
CAGCACGCTG ACATCTACAA GCTGCGGATT CGTGTTCGTG GCAACTTGGA GTGGGCCCCG 
CCCCGGCCTC AGATAATTTT TAATGTTCAT CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC 
AAGCAGAATT ACCGCTGTGC AGGATGTGGC ATCCGGACTG ACCCTGATTA CATCAAGCGA 
CTGCGGTACT GTGAGTACCT GGGCAAGTAC TTCTGCCAGT GCTGCCACGA GAATGCCCAG 
ATGGCCATCC CCAGCCGGGT TCTGCGCAAG TGGGACTTCA GCAAGTACTA CGTCAGCAAC 
TTCTCCAAGG ACCTGCTCAT TAAGATCTGG AATGATCCTC TCTTCAACGT GCAGGACATA 
AACAGTGCCC TCTATAGGAA GGTCAAGCTG CTCAATCAAG TCCGGCTGCT GCGGGTCCAG 
CTGTGTCACA TGAAGAACAT GTTCAAGACT TGCCGACTGG CCAAGGAGCT TCTGGATTCC 
TTTGACACAG TCCCAGGCCA CCTGACAGAG GACCTCCACC TGTACTCACT GAATGACCTG 
ACTGCGACCA GGAAGGGGGA GCTGGGGCCC CGGCTTGCTG AGCTCACCAG GGCAGGGGCT 
ACCCATGTGG AGAGATGCAT GCTCTGCCAA GCCAAAGGCT TCATCTGTGA GTTCTGTCAG 
AATGAGGATG ACATCATCTT TCCCTTTGAG CTCCATAAGT GCCGGACCTG TGAAGAGTGT 
AAAGCGTGTT ACCATAAAGC CTGCTTCAAG TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG 
CAGGCCCGGC GGGAGGCACT GGCCAGGCAG AGCCTGGAGT CTTACCTGTC AGACTACGAG 
GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713891.1
CDS	203..3196
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713828.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713828.3

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GAATCCTGCA ATGATAAGGC GAAGTTGAGA GGCCCTTTGC CCTACTCTGG TCAAAGCAGT 
GAAGTCAGCA CACCCAGCTC TCTGTACATG GAATATGAAG GTGGTCGGTA CCTGTGCTCA 
GGGGAAGGCA TGTTCCGAAG ACCATCAGAA GGACAGTCCC TCATCAGCTA CCTCTCTGAG 
CAAGACTTCG GCAGCTGTGC CGACCTGGAA AAGGAGAATG CCCACTTCAG CATCTCAGAG 
TCCTTAATTG CTGCCATCGA GCTAATGAAG TGCAACATGA TGAGCCAGTG CCTAGAGGAG 
GAGGAAGTGG AAGAGGAAGA CAGTGATAGA GAGATCCAGG AGCTGAAGCA GAAGATCCGC 
CTTCGGCGCC AGCAAATCCG CACCAAGAAC CTGCTCCCCA TGTACCAGGA GGCTGAGCAC 
GGAAGCTTTC GGGTCACCTC CAGCAGCTCC CAGTTCAGCT CACGTGATTC GGCACAGCTC 
TCTGACTCTG GCTCTGCTGA TGAGGTTGAT GAATTTGAAA TCCAAGATGG TAGCGAAGGA 
TCTAACCTGA CTCACATATC AAAGAACGGA CTGTCAGTGT CACTGGCCTC GATGTTCTCA 
GATGCTGACA TCAGAAGGAA CACAGCCTCA AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC 
TTCTCCCACT GCTTCCTGCA CTCCACGTCT GCTGAGGCGG TGGCCATGGG GCTCCTGAAG 
CAGTTTGAGG GGATGCAGCT TCCAGCCGCC TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT 
GATGCCCCTC AGAAGCTCCT GCCCATTCCT GACTCACTGC CCATCTCACC GGATGACGGG 
CAGCACGCTG ACATCTACAA GCTGCGGATT CGTGTTCGTG GCAACTTGGA GTGGGCCCCG 
CCCCGGCCTC AGATAATTTT TAATGTTCAT CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC 
AAGCAGAATT ACCGCTGTGC AGGATGTGGC ATCCGGACTG ACCCTGATTA CATCAAGCGA 
CTGCGGTACT GTGAGTACCT GGGCAAGTAC TTCTGCCAGT GCTGCCACGA GAATGCCCAG 
ATGGCCATCC CCAGCCGGGT TCTGCGCAAG TGGGACTTCA GCAAGTACTA CGTCAGCAAC 
TTCTCCAAGG ACCTGCTCAT TAAGATCTGG AATGATCCTC TCTTCAACGT GCAGGACATA 
AACAGTGCCC TCTATAGGAA GGTCAAGCTG CTCAATCAAG TCCGGCTGCT GCGGGTCCAG 
CTGTGTCACA TGAAGAACAT GTTCAAGACT TGCCGACTGG CCAAGGAGCT TCTGGATTCC 
TTTGACACAG TCCCAGGCCA CCTGACAGAG GACCTCCACC TGTACTCACT GAATGACCTG 
ACTGCGACCA GGAAGGGGGA GCTGGGGCCC CGGCTTGCTG AGCTCACCAG GGCAGGGGCT 
ACCCATGTGG AGAGATGCAT GCTCTGCCAA GCCAAAGGCT TCATCTGTGA GTTCTGTCAG 
AATGAGGATG ACATCATCTT TCCCTTTGAG CTCCATAAGT GCCGGACCTG TGAAGAGTGT 
AAAGCGTGTT ACCATAAAGC CTGCTTCAAG TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG 
CAGGCCCGGC GGGAGGCACT GGCCAGGCAG AGCCTGGAGT CTTACCTGTC AGACTACGAG 
GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_005269374.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269431.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X3, mRNA.

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CloneID	OHu38259
Clone ID Related Accession (Same CDS sequence)	XM_005269374.3
Accession Version	XM_005269374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA 
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT 
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC 
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT 
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT 
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT 
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC 
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC 
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG 
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG 
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG 
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT 
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG 
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC 
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA 
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG 
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG 
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG 
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA 
GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269431.1
CDS	203..3166
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_005269374.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269374.3

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGCTGACA TCAGAAGGAA CACAGCCTCA 
AGCAGCAAAT CCTTCGTTTC CTCCCAGTCC TTCTCCCACT GCTTCCTGCA CTCCACGTCT 
GCTGAGGCGG TGGCCATGGG GCTCCTGAAG CAGTTTGAGG GGATGCAGCT TCCAGCCGCC 
TCGGAGCTGG AGTGGCTTGT CCCGGAGCAT GATGCCCCTC AGAAGCTCCT GCCCATTCCT 
GACTCACTGC CCATCTCACC GGATGACGGG CAGCACGCTG ACATCTACAA GCTGCGGATT 
CGTGTTCGTG GCAACTTGGA GTGGGCCCCG CCCCGGCCTC AGATAATTTT TAATGTTCAT 
CCAGCCCCAA CGAGGAAAAT TGCCGTGGCC AAGCAGAATT ACCGCTGTGC AGGATGTGGC 
ATCCGGACTG ACCCTGATTA CATCAAGCGA CTGCGGTACT GTGAGTACCT GGGCAAGTAC 
TTCTGCCAGT GCTGCCACGA GAATGCCCAG ATGGCCATCC CCAGCCGGGT TCTGCGCAAG 
TGGGACTTCA GCAAGTACTA CGTCAGCAAC TTCTCCAAGG ACCTGCTCAT TAAGATCTGG 
AATGATCCTC TCTTCAACGT GCAGGACATA AACAGTGCCC TCTATAGGAA GGTCAAGCTG 
CTCAATCAAG TCCGGCTGCT GCGGGTCCAG CTGTGTCACA TGAAGAACAT GTTCAAGACT 
TGCCGACTGG CCAAGGAGCT TCTGGATTCC TTTGACACAG TCCCAGGCCA CCTGACAGAG 
GACCTCCACC TGTACTCACT GAATGACCTG ACTGCGACCA GGAAGGGGGA GCTGGGGCCC 
CGGCTTGCTG AGCTCACCAG GGCAGGGGCT ACCCATGTGG AGAGATGCAT GCTCTGCCAA 
GCCAAAGGCT TCATCTGTGA GTTCTGTCAG AATGAGGATG ACATCATCTT TCCCTTTGAG 
CTCCATAAGT GCCGGACCTG TGAAGAGTGT AAAGCGTGTT ACCATAAAGC CTGCTTCAAG 
TCTGGAAGCT GTCCGCGCTG CGAGCGGCTG CAGGCCCGGC GGGAGGCACT GGCCAGGCAG 
AGCCTGGAGT CTTACCTGTC AGACTACGAG GAGGAGCCCG CGGAAGCGCT GGCCCTGGAA 
GCCGCCGTCC TGGAGGCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713829.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713892.1 Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu38260
Clone ID Related Accession (Same CDS sequence)	XM_006713829.3 , XM_006713829.4
Accession Version	XM_006713829.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713892.1
CDS	699..3557
Translation	MQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713829.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713829.3

ORF Insert Sequence:

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713829.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713892.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X4, mRNA.

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CloneID	OHu38260
Clone ID Related Accession (Same CDS sequence)	XM_006713829.3 , XM_006713829.4
Accession Version	XM_006713829.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713829.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713892.1
CDS	547..3405
Translation	MQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713829.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713829.4

ORF Insert Sequence:

ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713831.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713894.1 Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu38261
Clone ID Related Accession (Same CDS sequence)	XM_006713831.3 , XM_006713831.4 , XM_006713830.3
Accession Version	XM_006713831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713894.1
CDS	866..3403
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713831.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713831.3

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713831.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713894.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu38261
Clone ID Related Accession (Same CDS sequence)	XM_006713831.3 , XM_006713831.4 , XM_006713830.3
Accession Version	XM_006713831.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006713831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713894.1
CDS	742..3279
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713831.4
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713831.4

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713830.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713893.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu38261
Clone ID Related Accession (Same CDS sequence)	XM_006713831.3 , XM_006713831.4 , XM_006713830.3
Accession Version	XM_006713830.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713893.1
CDS	553..3090
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713830.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713830.3

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGGTAGCGA AGGATCTAAC CTGACTCACA TATCAAAGAA CGGACTGTCA 
GTGTCACTGG CCTCGATGTT CTCAGATGCT GACATCAGAA GGAACACAGC CTCAAGCAGC 
AAATCCTTCG TTTCCTCCCA GTCCTTCTCC CACTGCTTCC TGCACTCCAC GTCTGCTGAG 
GCGGTGGCCA TGGGGCTCCT GAAGCAGTTT GAGGGGATGC AGCTTCCAGC CGCCTCGGAG 
CTGGAGTGGC TTGTCCCGGA GCATGATGCC CCTCAGAAGC TCCTGCCCAT TCCTGACTCA 
CTGCCCATCT CACCGGATGA CGGGCAGCAC GCTGACATCT ACAAGCTGCG GATTCGTGTT 
CGTGGCAACT TGGAGTGGGC CCCGCCCCGG CCTCAGATAA TTTTTAATGT TCATCCAGCC 
CCAACGAGGA AAATTGCCGT GGCCAAGCAG AATTACCGCT GTGCAGGATG TGGCATCCGG 
ACTGACCCTG ATTACATCAA GCGACTGCGG TACTGTGAGT ACCTGGGCAA GTACTTCTGC 
CAGTGCTGCC ACGAGAATGC CCAGATGGCC ATCCCCAGCC GGGTTCTGCG CAAGTGGGAC 
TTCAGCAAGT ACTACGTCAG CAACTTCTCC AAGGACCTGC TCATTAAGAT CTGGAATGAT 
CCTCTCTTCA ACGTGCAGGA CATAAACAGT GCCCTCTATA GGAAGGTCAA GCTGCTCAAT 
CAAGTCCGGC TGCTGCGGGT CCAGCTGTGT CACATGAAGA ACATGTTCAA GACTTGCCGA 
CTGGCCAAGG AGCTTCTGGA TTCCTTTGAC ACAGTCCCAG GCCACCTGAC AGAGGACCTC 
CACCTGTACT CACTGAATGA CCTGACTGCG ACCAGGAAGG GGGAGCTGGG GCCCCGGCTT 
GCTGAGCTCA CCAGGGCAGG GGCTACCCAT GTGGAGAGAT GCATGCTCTG CCAAGCCAAA 
GGCTTCATCT GTGAGTTCTG TCAGAATGAG GATGACATCA TCTTTCCCTT TGAGCTCCAT 
AAGTGCCGGA CCTGTGAAGA GTGTAAAGCG TGTTACCATA AAGCCTGCTT CAAGTCTGGA 
AGCTGTCCGC GCTGCGAGCG GCTGCAGGCC CGGCGGGAGG CACTGGCCAG GCAGAGCCTG 
GAGTCTTACC TGTCAGACTA CGAGGAGGAG CCCGCGGAAG CGCTGGCCCT GGAAGCCGCC 
GTCCTGGAGG CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_017007543.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863032.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu94800
Clone ID Related Accession (Same CDS sequence)	XM_017007543.1 , XM_024453838.1
Accession Version	XM_017007543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG 
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT 
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA 
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC 
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG 
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC 
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC 
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC 
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT 
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA 
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC 
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC 
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT 
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG 
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG 
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT 
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA 
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG 
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT 
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT 
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG 
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG 
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC 
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG 
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG 
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT 
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC 
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG 
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG 
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863032.1
CDS	549..3041
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_017007543.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007543.1

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG 
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT 
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA 
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC 
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG 
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC 
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC 
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC 
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT 
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA 
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC 
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC 
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT 
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG 
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG 
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT 
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA 
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG 
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT 
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT 
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG 
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG 
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC 
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG 
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG 
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT 
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC 
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG 
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG 
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_024453838.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309606.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu94800
Clone ID Related Accession (Same CDS sequence)	XM_017007543.1 , XM_024453838.1
Accession Version	XM_024453838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG 
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT 
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA 
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC 
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG 
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC 
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC 
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC 
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT 
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA 
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC 
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC 
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT 
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG 
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG 
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT 
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA 
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG 
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT 
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT 
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG 
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG 
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC 
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG 
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG 
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT 
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC 
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG 
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG 
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309606.1
CDS	742..3234
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_024453838.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453838.1

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG AATCCTGCAA TGATAAGGCG 
AAGTTGAGAG GCCCTTTGCC CTACTCTGGT CAAAGCAGTG AAGTCAGCAC ACCCAGCTCT 
CTGTACATGG AATATGAAGG TGGTCGGTAC CTGTGCTCAG GGGAAGGCAT GTTCCGAAGA 
CCATCAGAAG GACAGTCCCT CATCAGCTAC CTCTCTGAGC AAGACTTCGG CAGCTGTGCC 
GACCTGGAAA AGGAGAATGC CCACTTCAGC ATCTCAGAGT CCTTAATTGC TGCCATCGAG 
CTAATGAAGT GCAACATGAT GAGCCAGTGC CTAGAGGAGG AGGAAGTGGA AGAGGAAGAC 
AGTGATAGAG AGATCCAGGA GCTGAAGCAG AAGATCCGCC TTCGGCGCCA GCAAATCCGC 
ACCAAGAACC TGCTCCCCAT GTACCAGGAG GCTGAGCACG GAAGCTTTCG GGTCACCTCC 
AGCAGCTCCC AGTTCAGCTC ACGTGATTCG GCACAGCTCT CTGACTCTGG CTCTGCTGAT 
GAGGTTGATG AATTTGAAAT CCAAGATGGT AGCGAAGGAT CTAACCTGAC TCACATATCA 
AAGAACGGAC TGTCAGTGTC ACTGGCCTCG ATGTTCTCAG ATGCTGACAT CAGAAGGAAC 
ACAGCCTCAA GCAGCAAATC CTTCGTTTCC TCCCAGTCCT TCTCCCACTG CTTCCTGCAC 
TCCACGTCTG CTGAGGCGGT GGCCATGGGG CTCCTGAAGC AGTTTGAGGG GATGCAGCTT 
CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC CCGGAGCATG ATGCCCCTCA GAAGCTCCTG 
CCCATTCCTG ACTCACTGCC CATCTCACCG GATGACGGGC AGCACGCTGA CATCTACAAG 
CTGCGGATTC GTGTTCGTGG CAACTTGGAG TGGGCCCCGC CCCGGCCTCA GATAATTTTT 
AATGTTCATC CAGCCCCAAC GAGGAAAATT GCCGTGGCCA AGCAGAATTA CCGCTGTGCA 
GGATGTGGCA TCCGGACTGA CCCTGATTAC ATCAAGCGAC TGCGGTACTG TGAGTACCTG 
GGCAAGTACT TCTGCCAGTG CTGCCACGAG AATGCCCAGA TGGCCATCCC CAGCCGGGTT 
CTGCGCAAGT GGGACTTCAG CAAGTACTAC GTCAGCAACT TCTCCAAGGA CCTGCTCATT 
AAGATCTGGA ATGATCCTCT CTTCAACGTG CAGGACATAA ACAGTGCCCT CTATAGGAAG 
GTCAAGCTGC TCAATCAAGT CCGGCTGCTG CGGGTCCAGC TGTGTCACAT GAAGAACATG 
TTCAAGACTT GCCGACTGGC CAAGGAGCTT CTGGATTCCT TTGACACAGT CCCAGGCCAC 
CTGACAGAGG ACCTCCACCT GTACTCACTG AATGACCTGA CTGCGACCAG GAAGGGGGAG 
CTGGGGCCCC GGCTTGCTGA GCTCACCAGG GCAGGGGCTA CCCATGTGGA GAGATGCATG 
CTCTGCCAAG CCAAAGGCTT CATCTGTGAG TTCTGTCAGA ATGAGGATGA CATCATCTTT 
CCCTTTGAGC TCCATAAGTG CCGGACCTGT GAAGAGTGTA AAGCGTGTTA CCATAAAGCC 
TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC GAGCGGCTGC AGGCCCGGCG GGAGGCACTG 
GCCAGGCAGA GCCTGGAGTC TTACCTGTCA GACTACGAGG AGGAGCCCGC GGAAGCGCTG 
GCCCTGGAAG CCGCCGTCCT GGAGGCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_017007545.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863034.1 Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu94801
Clone ID Related Accession (Same CDS sequence)	XM_017007545.1 , XM_017007545.2 , XM_017007544.1
Accession Version	XM_017007545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863034.1
CDS	866..3328
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_017007545.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RUN and cysteine rich domain containing beclin 1 interacting protein (RUBCN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007545.1

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_017007545.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863034.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu94801
Clone ID Related Accession (Same CDS sequence)	XM_017007545.1 , XM_017007545.2 , XM_017007544.1
Accession Version	XM_017007545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863034.1
CDS	742..3204
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_017007545.2
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007545.2

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_017007544.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863033.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X9, mRNA.

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CloneID	OHu94801
Clone ID Related Accession (Same CDS sequence)	XM_017007545.1 , XM_017007545.2 , XM_017007544.1
Accession Version	XM_017007544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863033.1
CDS	550..3012
Translation	MLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_017007544.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007544.1

ORF Insert Sequence:

ATGCTGCAGT GCCTGGAAGC AGTGGAACAG AACAACCCCC GCCTCCTGGC TCAGATCGAT 
GCGTCCATGT TTGCCAGAAA GCACGAGAGC CCGCTCCTGG TGACAAAGAG CCAGAGCCTG 
ACAGCCCTGC CCAGTTCCAC ATACACCCCT CCAAACAGCT ATGCTCAGCA TTCCTACTTT 
GGGTCCTTCT CTAGCCTCCA CCAATCCGTG CCCAACAATG GCTCAGAGAG AAGATCTACT 
TCCTTTCCAC TCTCTGGCCC TCCCCGGAAA CCTCAAGAAA GCAGAGGGCA CGTCTCACCA 
GCAGAGGATC AAACCATCCA AGCCCCCCCA GTTTCAGTCT CTGCACTAGC CAGGGATTCC 
CCTTTGACCC CAAATGAAAT GAGCTCCAGT ACTCTGACCA GCCCCATAGA GGCATCCTGG 
GTCAGCAGCC AGAATGATTC CCCAGGTGAT GCCAGTGAGG GGCCTGAGTA CCTGGCCATT 
GGCAACTTGG ACCCCCGAGG CCGGACTGCC AGCTGTCAGA GTCACAGCAG CAATGCCGAG 
AGCAGCAGTT CCAATTTGTT CTCCTCCAGC AGCTCCCAGA AGCCAGATTC TGCTGCCTCT 
TCCTTAGGGG ACCAGGAAGG AGGTGGGGAG AGCCAGCTGT CCAGTGTCCT CCGCAGGTCC 
AGCTTCTCAG AGGGGCAGAC ACTCACTGTC ACCAGTGGGG CAAAGAAAAG CCACATTCGC 
TCCCATTCGG ATACCAGCAT TGCCTCCAGG GGAGCTCCAG GAGGCCCCAG GAATATCACC 
ATTATAGTTG AAGATCCCAT TGCAGAATCC TGCAATGATA AGGCGAAGTT GAGAGGCCCT 
TTGCCCTACT CTGGTCAAAG CAGTGAAGTC AGCACACCCA GCTCTCTGTA CATGGAATAT 
GAAGGTGGTC GGTACCTGTG CTCAGGGGAA GGCATGTTCC GAAGACCATC AGAAGGACAG 
TCCCTCATCA GCTACCTCTC TGAGCAAGAC TTCGGCAGCT GTGCCGACCT GGAAAAGGAG 
AATGCCCACT TCAGCATCTC AGAGTCCTTA ATTGCTGCCA TCGAGCTAAT GAAGTGCAAC 
ATGATGAGCC AGTGCCTAGA GGAGGAGGAA GTGGAAGAGG AAGACAGTGA TAGAGAGATC 
CAGGAGCTGA AGCAGAAGAT CCGCCTTCGG CGCCAGCAAA TCCGCACCAA GAACCTGCTC 
CCCATGTACC AGGAGGCTGA GCACGGAAGC TTTCGGGTCA CCTCCAGCAG CTCCCAGTTC 
AGCTCACGTG ATTCGGCACA GCTCTCTGAC TCTGGCTCTG CTGATGAGGT TGATGAATTT 
GAAATCCAAG ATGCTGACAT CAGAAGGAAC ACAGCCTCAA GCAGCAAATC CTTCGTTTCC 
TCCCAGTCCT TCTCCCACTG CTTCCTGCAC TCCACGTCTG CTGAGGCGGT GGCCATGGGG 
CTCCTGAAGC AGTTTGAGGG GATGCAGCTT CCAGCCGCCT CGGAGCTGGA GTGGCTTGTC 
CCGGAGCATG ATGCCCCTCA GAAGCTCCTG CCCATTCCTG ACTCACTGCC CATCTCACCG 
GATGACGGGC AGCACGCTGA CATCTACAAG CTGCGGATTC GTGTTCGTGG CAACTTGGAG 
TGGGCCCCGC CCCGGCCTCA GATAATTTTT AATGTTCATC CAGCCCCAAC GAGGAAAATT 
GCCGTGGCCA AGCAGAATTA CCGCTGTGCA GGATGTGGCA TCCGGACTGA CCCTGATTAC 
ATCAAGCGAC TGCGGTACTG TGAGTACCTG GGCAAGTACT TCTGCCAGTG CTGCCACGAG 
AATGCCCAGA TGGCCATCCC CAGCCGGGTT CTGCGCAAGT GGGACTTCAG CAAGTACTAC 
GTCAGCAACT TCTCCAAGGA CCTGCTCATT AAGATCTGGA ATGATCCTCT CTTCAACGTG 
CAGGACATAA ACAGTGCCCT CTATAGGAAG GTCAAGCTGC TCAATCAAGT CCGGCTGCTG 
CGGGTCCAGC TGTGTCACAT GAAGAACATG TTCAAGACTT GCCGACTGGC CAAGGAGCTT 
CTGGATTCCT TTGACACAGT CCCAGGCCAC CTGACAGAGG ACCTCCACCT GTACTCACTG 
AATGACCTGA CTGCGACCAG GAAGGGGGAG CTGGGGCCCC GGCTTGCTGA GCTCACCAGG 
GCAGGGGCTA CCCATGTGGA GAGATGCATG CTCTGCCAAG CCAAAGGCTT CATCTGTGAG 
TTCTGTCAGA ATGAGGATGA CATCATCTTT CCCTTTGAGC TCCATAAGTG CCGGACCTGT 
GAAGAGTGTA AAGCGTGTTA CCATAAAGCC TGCTTCAAGT CTGGAAGCTG TCCGCGCTGC 
GAGCGGCTGC AGGCCCGGCG GGAGGCACTG GCCAGGCAGA GCCTGGAGTC TTACCTGTCA 
GACTACGAGG AGGAGCCCGC GGAAGCGCTG GCCCTGGAAG CCGCCGTCCT GGAGGCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( NM_001346873.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333802.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu100758
Clone ID Related Accession (Same CDS sequence)	NM_001346873.1
Accession Version	NM_001346873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC024560.26. This sequence is a reference standard in the RefSeqGene project. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.1208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGGTGGTCGG TACCTGTGCT CAGGGGAAGG CATGTTCCGA 
AGACCATCAG AAGGACAGTC CCTCATCAGC TACCTCTCTG AGCAAGACTT CGGCAGCTGT 
GCCGACCTGG AAAAGGAGAA TGCCCACTTC AGCATCTCAG AGTCCTTAAT TGCTGCCATC 
GAGCTAATGA AGTGCAACAT GATGAGCCAG TGCCTAGAGG AGGAGGAAGT GGAAGAGGAA 
GACAGTGATA GAGAGATCCA GGAGCTGAAG CAGAAGATCC GCCTTCGGCG CCAGCAAATC 
CGCACCAAGA ACCTGCTCCC CATGTACCAG GAGGCTGAGC ACGGAAGCTT TCGGGTCACC 
TCCAGCAGCT CCCAGTTCAG CTCACGTGAT TCGGCACAGC TCTCTGACTC TGGCTCTGCT 
GATGAGGTTG ATGAATTTGA AATCCAAGAT GGTAGCGAAG GATCTAACCT GACTCACATA 
TCAAAGAACG GACTGTCAGT GTCACTGGCC TCGATGTTCT CAGATGCTGA CATCAGAAGG 
AACACAGCCT CAAGCAGCAA ATCCTTCGTT TCCTCCCAGT CCTTCTCCCA CTGCTTCCTG 
CACTCCACGT CTGCTGAGGC GGTGGCCATG GGGCTCCTGA AGCAGTTTGA GGGGATGCAG 
CTTCCAGCCG CCTCGGAGCT GGAGTGGCTT GTCCCGGAGC ATGATGCCCC TCAGAAGCTC 
CTGCCCATTC CTGACTCACT GCCCATCTCA CCGGATGACG GGCAGCACGC TGACATCTAC 
AAGCTGCGGA TTCGTGTTCG TGGCAACTTG GAGTGGGCCC CGCCCCGGCC TCAGATAATT 
TTTAATGTTC ATCCAGCCCC AACGAGGAAA ATTGCCGTGG CCAAGCAGAA TTACCGCTGT 
GCAGGATGTG GCATCCGGAC TGACCCTGAT TACATCAAGC GACTGCGGTA CTGTGAGTAC 
CTGGGCAAGT ACTTCTGCCA GTGCTGCCAC GAGAATGCCC AGATGGCCAT CCCCAGCCGG 
GTTCTGCGCA AGTGGGACTT CAGCAAGTAC TACGTCAGCA ACTTCTCCAA GGACCTGCTC 
ATTAAGATCT GGAATGATCC TCTCTTCAAC GTGCAGGACA TAAACAGTGC CCTCTATAGG 
AAGGTCAAGC TGCTCAATCA AGTCCGGCTG CTGCGGGTCC AGCTGTGTCA CATGAAGAAC 
ATGTTCAAGA CTTGCCGACT GGCCAAGGAG CTTCTGGATT CCTTTGACAC AGTCCCAGGC 
CACCTGACAG AGGACCTCCA CCTGTACTCA CTGAATGACC TGACTGCGAC CAGGAAGGGG 
GAGCTGGGGC CCCGGCTTGC TGAGCTCACC AGGGCAGGGG CTACCCATGT GGAGAGATGC 
ATGCTCTGCC AAGCCAAAGG CTTCATCTGT GAGTTCTGTC AGAATGAGGA TGACATCATC 
TTTCCCTTTG AGCTCCATAA GTGCCGGACC TGTGAAGAGT GTAAAGCGTG TTACCATAAA 
GCCTGCTTCA AGTCTGGAAG CTGTCCGCGC TGCGAGCGGC TGCAGGCCCG GCGGGAGGCA 
CTGGCCAGGC AGAGCCTGGA GTCTTACCTG TCAGACTACG AGGAGGAGCC CGCGGAAGCG 
CTGGCCCTGG AAGCCGCCGT CCTGGAGGCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333802.1
CDS	208..3243
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	NM_001346873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346873.1

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGGTGGTCGG TACCTGTGCT CAGGGGAAGG CATGTTCCGA 
AGACCATCAG AAGGACAGTC CCTCATCAGC TACCTCTCTG AGCAAGACTT CGGCAGCTGT 
GCCGACCTGG AAAAGGAGAA TGCCCACTTC AGCATCTCAG AGTCCTTAAT TGCTGCCATC 
GAGCTAATGA AGTGCAACAT GATGAGCCAG TGCCTAGAGG AGGAGGAAGT GGAAGAGGAA 
GACAGTGATA GAGAGATCCA GGAGCTGAAG CAGAAGATCC GCCTTCGGCG CCAGCAAATC 
CGCACCAAGA ACCTGCTCCC CATGTACCAG GAGGCTGAGC ACGGAAGCTT TCGGGTCACC 
TCCAGCAGCT CCCAGTTCAG CTCACGTGAT TCGGCACAGC TCTCTGACTC TGGCTCTGCT 
GATGAGGTTG ATGAATTTGA AATCCAAGAT GGTAGCGAAG GATCTAACCT GACTCACATA 
TCAAAGAACG GACTGTCAGT GTCACTGGCC TCGATGTTCT CAGATGCTGA CATCAGAAGG 
AACACAGCCT CAAGCAGCAA ATCCTTCGTT TCCTCCCAGT CCTTCTCCCA CTGCTTCCTG 
CACTCCACGT CTGCTGAGGC GGTGGCCATG GGGCTCCTGA AGCAGTTTGA GGGGATGCAG 
CTTCCAGCCG CCTCGGAGCT GGAGTGGCTT GTCCCGGAGC ATGATGCCCC TCAGAAGCTC 
CTGCCCATTC CTGACTCACT GCCCATCTCA CCGGATGACG GGCAGCACGC TGACATCTAC 
AAGCTGCGGA TTCGTGTTCG TGGCAACTTG GAGTGGGCCC CGCCCCGGCC TCAGATAATT 
TTTAATGTTC ATCCAGCCCC AACGAGGAAA ATTGCCGTGG CCAAGCAGAA TTACCGCTGT 
GCAGGATGTG GCATCCGGAC TGACCCTGAT TACATCAAGC GACTGCGGTA CTGTGAGTAC 
CTGGGCAAGT ACTTCTGCCA GTGCTGCCAC GAGAATGCCC AGATGGCCAT CCCCAGCCGG 
GTTCTGCGCA AGTGGGACTT CAGCAAGTAC TACGTCAGCA ACTTCTCCAA GGACCTGCTC 
ATTAAGATCT GGAATGATCC TCTCTTCAAC GTGCAGGACA TAAACAGTGC CCTCTATAGG 
AAGGTCAAGC TGCTCAATCA AGTCCGGCTG CTGCGGGTCC AGCTGTGTCA CATGAAGAAC 
ATGTTCAAGA CTTGCCGACT GGCCAAGGAG CTTCTGGATT CCTTTGACAC AGTCCCAGGC 
CACCTGACAG AGGACCTCCA CCTGTACTCA CTGAATGACC TGACTGCGAC CAGGAAGGGG 
GAGCTGGGGC CCCGGCTTGC TGAGCTCACC AGGGCAGGGG CTACCCATGT GGAGAGATGC 
ATGCTCTGCC AAGCCAAAGG CTTCATCTGT GAGTTCTGTC AGAATGAGGA TGACATCATC 
TTTCCCTTTG AGCTCCATAA GTGCCGGACC TGTGAAGAGT GTAAAGCGTG TTACCATAAA 
GCCTGCTTCA AGTCTGGAAG CTGTCCGCGC TGCGAGCGGC TGCAGGCCCG GCGGGAGGCA 
CTGGCCAGGC AGAGCCTGGA GTCTTACCTG TCAGACTACG AGGAGGAGCC CGCGGAAGCG 
CTGGCCCTGG AAGCCGCCGT CCTGGAGGCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RUBCN ( XM_006713827.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713890.1 Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X1, mRNA.

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CloneID	OHu38257
Clone ID Related Accession (Same CDS sequence)	XM_006713827.3
Accession Version	XM_006713827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713890.1
CDS	204..3242
Translation	MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPGGPRNITIIVEDPIAESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDGSEGSNLTHISKNGLSVSLASMFSDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Target ORF information:

RefSeq Version	XM_006713827.3
Organism	Homo sapiens(human)
Definition	Homo sapiens rubicon autophagy regulator (RUBCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713827.3

ORF Insert Sequence:

ATGCGGCCGG AGGGCGCGGG AATGGAGCTC GGAGGCGGCG AGGAGCGCCT GCCTGAGGAG 
AGCAGGAGGG AGCACTGGCA GTTGCTGGGT AATTTGAAGA CGACGGTGGA GGGTTTGGTA 
TCAACCAACA GCCCCAACGT CTGGTCTAAG TATGGTGGCT TGGAGCGGCT TTGCAGGGAC 
ATGCAGAGCA TCCTCTATCA CGGGCTTATC CGTGACCAGG CGTGCCGCCG CCAGACGGAT 
TACTGGCAGT TCGTGAAAGA CATCCGGTGG CTCAGTCCCC ACTCAGCCCT TCACGTGGAG 
AAGTTCATCA GCGTGCACGA GAACGACCAG AGCAGTGCTG ATGGTGCCAG TGAACGTGCT 
GTTGCCGAGC TGTGGCTGCA GCACAGCCTG CAGTACCACT GCCTCTCAGC CCAGCTCCGG 
CCCCTGCTCG GGGATAGACA GTATATCAGA AAATTCTACA CAGATGCTGC CTTCCTGCTA 
AGTGACGCTC ATGTCACGGC CATGCTGCAG TGCCTGGAAG CAGTGGAACA GAACAACCCC 
CGCCTCCTGG CTCAGATCGA TGCGTCCATG TTTGCCAGAA AGCACGAGAG CCCGCTCCTG 
GTGACAAAGA GCCAGAGCCT GACAGCCCTG CCCAGTTCCA CATACACCCC TCCAAACAGC 
TATGCTCAGC ATTCCTACTT TGGGTCCTTC TCTAGCCTCC ACCAATCCGT GCCCAACAAT 
GGCTCAGAGA GAAGATCTAC TTCCTTTCCA CTCTCTGGCC CTCCCCGGAA ACCTCAAGAA 
AGCAGAGGGC ACGTCTCACC AGCAGAGGAT CAAACCATCC AAGCCCCCCC AGTTTCAGTC 
TCTGCACTAG CCAGGGATTC CCCTTTGACC CCAAATGAAA TGAGCTCCAG TACTCTGACC 
AGCCCCATAG AGGCATCCTG GGTCAGCAGC CAGAATGATT CCCCAGGTGA TGCCAGTGAG 
GGGCCTGAGT ACCTGGCCAT TGGCAACTTG GACCCCCGAG GCCGGACTGC CAGCTGTCAG 
AGTCACAGCA GCAATGCCGA GAGCAGCAGT TCCAATTTGT TCTCCTCCAG CAGCTCCCAG 
AAGCCAGATT CTGCTGCCTC TTCCTTAGGG GACCAGGAAG GAGGTGGGGA GAGCCAGCTG 
TCCAGTGTCC TCCGCAGGTC CAGCTTCTCA GAGGGGCAGA CACTCACTGT CACCAGTGGG 
GCAAAGAAAA GCCACATTCG CTCCCATTCG GATACCAGCA TTGCCTCCAG GGGAGCTCCA 
GGAGGCCCCA GGAATATCAC CATTATAGTT GAAGATCCCA TTGCAGAATC CTGCAATGAT 
AAGGCGAAGT TGAGAGGCCC TTTGCCCTAC TCTGGTCAAA GCAGTGAAGT CAGCACACCC 
AGCTCTCTGT ACATGGAATA TGAAGGTGGT CGGTACCTGT GCTCAGGGGA AGGCATGTTC 
CGAAGACCAT CAGAAGGACA GTCCCTCATC AGCTACCTCT CTGAGCAAGA CTTCGGCAGC 
TGTGCCGACC TGGAAAAGGA GAATGCCCAC TTCAGCATCT CAGAGTCCTT AATTGCTGCC 
ATCGAGCTAA TGAAGTGCAA CATGATGAGC CAGTGCCTAG AGGAGGAGGA AGTGGAAGAG 
GAAGACAGTG ATAGAGAGAT CCAGGAGCTG AAGCAGAAGA TCCGCCTTCG GCGCCAGCAA 
ATCCGCACCA AGAACCTGCT CCCCATGTAC CAGGAGGCTG AGCACGGAAG CTTTCGGGTC 
ACCTCCAGCA GCTCCCAGTT CAGCTCACGT GATTCGGCAC AGCTCTCTGA CTCTGGCTCT 
GCTGATGAGG TTGATGAATT TGAAATCCAA GATGGTAGCG AAGGATCTAA CCTGACTCAC 
ATATCAAAGA ACGGACTGTC AGTGTCACTG GCCTCGATGT TCTCAGATGC TGACATCAGA 
AGGAACACAG CCTCAAGCAG CAAATCCTTC GTTTCCTCCC AGTCCTTCTC CCACTGCTTC 
CTGCACTCCA CGTCTGCTGA GGCGGTGGCC ATGGGGCTCC TGAAGCAGTT TGAGGGGATG 
CAGCTTCCAG CCGCCTCGGA GCTGGAGTGG CTTGTCCCGG AGCATGATGC CCCTCAGAAG 
CTCCTGCCCA TTCCTGACTC ACTGCCCATC TCACCGGATG ACGGGCAGCA CGCTGACATC 
TACAAGCTGC GGATTCGTGT TCGTGGCAAC TTGGAGTGGG CCCCGCCCCG GCCTCAGATA 
ATTTTTAATG TTCATCCAGC CCCAACGAGG AAAATTGCCG TGGCCAAGCA GAATTACCGC 
TGTGCAGGAT GTGGCATCCG GACTGACCCT GATTACATCA AGCGACTGCG GTACTGTGAG 
TACCTGGGCA AGTACTTCTG CCAGTGCTGC CACGAGAATG CCCAGATGGC CATCCCCAGC 
CGGGTTCTGC GCAAGTGGGA CTTCAGCAAG TACTACGTCA GCAACTTCTC CAAGGACCTG 
CTCATTAAGA TCTGGAATGA TCCTCTCTTC AACGTGCAGG ACATAAACAG TGCCCTCTAT 
AGGAAGGTCA AGCTGCTCAA TCAAGTCCGG CTGCTGCGGG TCCAGCTGTG TCACATGAAG 
AACATGTTCA AGACTTGCCG ACTGGCCAAG GAGCTTCTGG ATTCCTTTGA CACAGTCCCA 
GGCCACCTGA CAGAGGACCT CCACCTGTAC TCACTGAATG ACCTGACTGC GACCAGGAAG 
GGGGAGCTGG GGCCCCGGCT TGCTGAGCTC ACCAGGGCAG GGGCTACCCA TGTGGAGAGA 
TGCATGCTCT GCCAAGCCAA AGGCTTCATC TGTGAGTTCT GTCAGAATGA GGATGACATC 
ATCTTTCCCT TTGAGCTCCA TAAGTGCCGG ACCTGTGAAG AGTGTAAAGC GTGTTACCAT 
AAAGCCTGCT TCAAGTCTGG AAGCTGTCCG CGCTGCGAGC GGCTGCAGGC CCGGCGGGAG 
GCACTGGCCA GGCAGAGCCT GGAGTCTTAC CTGTCAGACT ACGAGGAGGA GCCCGCGGAA 
GCGCTGGCCC TGGAAGCCGC CGTCCTGGAG GCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RUBCN
Entrez Gene ID	9711
Full Name	RUN and cysteine rich domain containing beclin 1 interacting protein
Synonyms	KIAA0226,RUBICON,SCAR15
General protein information	Preferred Names RUN and cysteine rich domain containing beclin 1 interacting protein Names run domain Beclin-1-interacting and cysteine-rich domain-containing protein RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein baron beclin-1 associated RUN domain containing protein rundataxin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q29
Summary	The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014].
Disorder MIM:	613516
Disorder Html:	?Spinocerebellar ataxia, autosomal recessive 15, 615705 (3)

Canis lupus familiaris (dog)	KIAA0226	XP_005639648.1
Bos taurus (cattle)	KIAA0226	NP_001095367.2
Gallus gallus (chicken)	KIAA0226	XP_004943414.1
Xenopus tropicalis (tropical clawed frog)	kiaa0226	XP_004914445.1
Mus musculus (house mouse)	1700021K19Rik	NP_001186967.1
Pan troglodytes (chimpanzee)	KIAA0226	XP_003950310.1
Danio rerio (zebrafish)	si:ch1073-280h16.1	XP_005174116.1
	LOC715781	XP_001106158.2
Homo sapiens (human)	KIAA0226	NP_055502.1
Rattus norvegicus (Norway rat)	RGD1305422	XP_006248530.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RUBCN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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