ESPL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	9700
Full Name	extra spindle pole bodies like 1, separase
Synonyms	ESP1,SEPA
General protein information	Preferred Names extra spindle pole bodies like 1, separase Names separin caspase-like protein ESPL1 extra spindle pole bodies 1, separase extra spindle pole bodies homolog 1 extra spindle poles like 1 extra spindle poles-like 1 protein separin, cysteine protease
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010].
Disorder MIM:	604143

mRNA and Protein(s)

mRNA	Protein	Name
XM_011539024.2	XP_011537326.1	separin isoform X1
NM_012291.4	NP_036423.4	separin
XM_017020253.1	XP_016875742.1	separin isoform X3
NM_012291.5	NP_036423.4	separin
XM_006719705.3	XP_006719768.1	separin isoform X1
XM_011539025.2	XP_011537327.1	separin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ESPL1	NP_036423.4
	ESPL1	XP_001103494.1
Danio rerio (zebrafish)	espl1	XP_001337869.1
Pan troglodytes (chimpanzee)	ESPL1	XP_003313754.1
Canis lupus familiaris (dog)	ESPL1	XP_849828.2
Bos taurus (cattle)	ESPL1	NP_001039414.2
Gallus gallus (chicken)	ESPL1	XP_423289.4
Mus musculus (house mouse)	Espl1	NP_001014976.1
Rattus norvegicus (Norway rat)	Espl1	NP_001164073.1
Xenopus tropicalis (tropical clawed frog)	espl1	XP_004912005.1

Gene Ontology
Bibliography

Related articles in PubMed

Separase is a marker for prognosis and mitotic activity in breast cancer.
Gurvits N, L?yttyniemi E, Nyk?nen M, Kuopio T, Kronqvist P, Talvinen K
British journal of cancer117(9)1383-1391(2017 Oct)

Separase: Function Beyond Cohesion Cleavage and an Emerging Oncogene.
Kumar R
Journal of cellular biochemistry118(6)1283-1299(2017 Jun)

Proximity mapping of human separase by the BioID approach.
Agircan FG, Hata S, Nussbaum-Krammer C, Atorino E, Schiebel E
Biochemical and biophysical research communications478(2)656-62(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High ESP expression is associated with breast cancer.
Title: Separase is a marker for prognosis and mitotic activity in breast cancer.

Studies identified and characterized the role of separase in mitosis, meiosis, non-canonical roles, its regulation, as a regulator of centriole disengagement, non proteolytic roles, diverse substrates, structural insights, and association of separase with cancer. [review]
Title: Separase: Function Beyond Cohesion Cleavage and an Emerging Oncogene.

Proximity mapping of human separase has been presented.
Title: Proximity mapping of human separase by the BioID approach.

The assay was used to quantify Separase proteolytic activity in leukemic cell lines and peripheral blood samples from leukemia patients.
Title: Measurement of separase proteolytic activity in single living cells by a fluorogenic flow cytometry assay.

Separase protein levels decrease and Separase proteolytic activity increases exclusively in b3a2 p210BCR-ABL-positive cell lines under Imatinib treatment.
Title: Clonal Evolution and Blast Crisis Correlate with Enhanced Proteolytic Activity of Separase in BCR-ABL b3a2 Fusion Type CML under Imatinib Therapy.

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu84339	XM_017020253.1 Latest version!	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu63185	XM_011539025.2 Latest version!	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu13319	NM_012291.5 Latest version!	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu13319	XM_011539024.2 Latest version!	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu13319	XM_006719705.3 Latest version!	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
View Old Accession Versions >>
OHu13319	NM_012291.4	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( NM_012291.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036423.4 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.

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CloneID	OHu13319
Clone ID Related Accession (Same CDS sequence)	XM_011539024.2 , NM_012291.4 , NM_012291.5 , XM_006719705.3
Accession Version	NM_012291.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	separin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP244662.1, AC021072.27, AY455930.1, AC073611.29 and AI458447.1. On Mar 29, 2007 this sequence version replaced NM_012291.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY455930.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036423.4
CDS	92..6454
Translation	MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	NM_012291.4
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012291.4

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_017020253.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875742.1 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

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CloneID	OHu84339
Clone ID Related Accession (Same CDS sequence)	XM_017020253.1
Accession Version	XM_017020253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAC CATCACCCCC ACTTCGGGCA TTGTATGAGA GCTGCCAGTT CTTCCTTTCA 
GGCCTGGAAC GAGGCACCAA GAGGCGCTAT AGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGAG GGTACTGCTC TCTTCTGCAG CAGCTGCGGG ATGATGGTGT GTATGGGGGC 
TCCTCCAAGC AACAGCAGTC TTTTCTTCAG ATGTACTTTC AGGGACTTCA CCTCTACACT 
GTGGTGGTTT ATGACTTTGC CCAAGGCTGT CAGATAGTTG ATTTGGCTGA CCTGACCCAA 
CTAGTGGACA GTTGTAAATC TACCGTTGTC TGGATGCTGG AGGCCTTAGA GGGCCTGTCG 
GGCCAAGAGC TGACGGACCA CATGGGGATG ACCGCTTCTT ACACCAGTAA TTTGGCCTAC 
AGCTTCTATA GTCACAAGCT CTATGCCGAG GCCTGTGCCA TCTCTGAGCC GCTCTGTCAG 
CACCTGGGTT TGGTGAAGCC AGGCACTTAT CCCGAGGTGC CTCCTGAGAA GTTGCACAGG 
TGCTTCCGGC TACAAGTAGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG 
ATGGTGATTT TGTGGCTGGC AGCCCTGCAA CCCTGTAGCC CTGAACACAT GGCTGAGCCA 
GTCACTTTCT GGGTTCGGGT CAAGATGGAT GCGGCCAGGG CTGGAGACAA GGAGCTACAG 
CTAAAGACTC TGCGAGACAG CCTCAGTGGC TGGGACCCGG AGACCCTGGC CCTCCTGCTG 
AGGGAGGAGC TGCAGGCCTA CAAGGCGGTG CGGGCCGACA CTGGACAGGA ACGCTTCAAC 
ATCATCTGTG ACCTCCTGGA GCTGAGCCCC GAGGAGACAC CAGCCGGGGC CTGGGCACGA 
GCCACCCACC TGGTAGAACT GGCTCAGGTG CTCTGCTACC ACGACTTTAC GCAGCAGACC 
AACTGCTCTG CTCTGGATGC TATCCGGGAA GCCCTGCAGC TTCTGGACTC TGTGAGGCCT 
GAGGCCCAGG CCAGAGATCA GCTTCTGGAC GATAAAGCAC AGGCCTTGCT GTGGCTTTAC 
ATCTGTACTC TGGAAGCCAA AATGCAGGAA GGTATCGAGC GGGATCGGAG AGCCCAGGCC 
CCTGGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTCCAGGAA 
GATCGTTTCC TATACAGTAA CATTGCCTTC AACCTGGCTG CAGATGCTGC TCAGTCCAAA 
TGCCTGGACC AAGCCCTGGC CCTGTGGAAG GAGCTGCTTA CAAAGGGGCA GGCCCCAGCT 
GTACGGTGTC TCCAGCAGAC AGCAGCCTCA CTGCAGATCC TAGCAGCCCT CTACCAGCTG 
GTGGCAAAGC CCATGCAGGC TCTGGAGGTC CTCCTGCTGC TACGGATTGT CTCTGAGAGA 
CTGAAGGACC ACTCGAAGGC AGCTGGCTCC TCCTGCCACA TCACCCAGCT CCTCCTGACC 
CTCGGCTGTC CCAGCTATGC CCAGTTACAC CTGGAAGAGG CAGCATCGAG CCTGAAGCAT 
CTCGATCAGA CTACTGACAC ATACCTGCTC CTTTCCCTGA CCTGTGATCT GCTTCGAAGT 
CAACTCTACT GGACTCACCA GAAGGTGACC AAGGGTGTCT CTCTGCTGCT GTCTGTGCTT 
CGGGATCCTG CCCTCCAGAA GTCCTCCAAG GCTTGGTACT TGCTGCGTGT CCAGGTCCTG 
CAGCTGGTGG CAGCTTACCT TAGCCTCCCG TCAAACAACC TCTCACACTC CCTGTGGGAG 
CAGCTCTGTG CCCAAGGCTG GCAGACACCT GAGATAGCTC TCATAGACTC CCATAAGCTC 
CTCCGAAGCA TCATCCTCCT GCTGATGGGC AGTGACATTC TCTCAACTCA GAAAGCAGCT 
GTGGAGACAT CGTTTTTGGA CTATGGTGAA AATCTGGTAC AAAAATGGCA GGTTCTTTCA 
GAGGTGCTGA GCTGCTCAGA GAAGCTGGTC TGCCACCTGG GCCGCCTGGG TAGTGTGAGT 
GAAGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACAA CAAAGCTGCA GATACCACGC 
CAGTGTGCCC TGTTCCTGGT GCTGAAGGGC GAGCTGGAGC TGGCCCGCAA TGACATTGAT 
CTCTGTCAGT CGGACCTGCA GCAGGTTCTG TTCTTGCTTG AGTCTTGCAC AGAGTTTGGT 
GGGGTGACTC AGCACCTGGA CTCTGTGAAG AAGGTCCACC TGCAGAAGGG GAAGCAGCAG 
GCCCAGGTCC CCTGTCCTCC ACAGCTCCCA GAGGAGGAGC TCTTCCTAAG AGGCCCTGCT 
CTAGAGCTGG TGGCCACTGT GGCCAAGGAG CCTGGCCCCA TAGCACCTTC TACAAACTCC 
TCCCCAGTCT TGAAAACCAA GCCCCAGCCC ATACCCAACT TCCTGTCCCA TTCACCCACC 
TGTGACTGCT CGCTCTGCGC CAGCCCTGTC CTCACAGCAG TCTGTCTGCG CTGGGTATTG 
GTCACGGCAG GGGTGAGGCT GGCCATGGGC CACCAAGCCC AGGGTCTGGA TCTGCTGCAG 
GTCGTGCTGA AGGGCTGTCC TGAAGCCGCT GAGCGCCTCA CCCAAGCTCT CCAAGCTTCC 
CTGAATCATA AAACACCCCC CTCCTTGGTT CCAAGCCTCT TGGATGAGAT CTTGGCTCAA 
GCATACACAC TGTTGGCACT GGAGGGCCTG AACCAGCCAT CAAACGAGAG CCTGCAGAAG 
GTTCTACAGT CAGGGCTGAA GTTTGTAGCA GCACGGATAC CCCACCTAGA GCCCTGGCGA 
GCCAGCCTGC TCTTGATTTG GGCCCTCACA AAACTAGGTG GCCTCAGCTG CTGTACTACC 
CAACTTTTTG CAAGCTCCTG GGGCTGGCAG CCACCATTAA TAAAAAGTGT CCCTGGCTCA 
GAGCCCTCTA AGACTCAGGG CCAAAAACGT TCTGGACGAG GGCGCCAAAA GTTAGCCTCT 
GCTCCCCTGC GCCTCAATAA TACCTCTCAG AAAGGTCTGG AAGGTAGAGG ACTGCCCTGC 
ACACCTAAAC CCCCAGACCG GATCAGGCAA GCTGGCCCTC ATGTCCCCTT CACGGTGTTT 
GAGGAAGTCT GCCCTACAGA GAGCAAGCCT GAAGTACCCC AGGCCCCCAG GGTACAACAG 
AGAGTCCAGA CGCGCCTCAA GGTGAACTTC AGTGATGACA GTGACTTGGA AGACCCTGTC 
TCAGCTGAGG CCTGGCTGGC AGAGGAGCCT AAGAGACGGG GCACTGCTTC CCGGGGCCGG 
GGGCGAGCAA GGAAGGGCCT GAGCCTAAAG ACGGATGCCG TGGTTGCCCC AGGTAGTGCC 
CCTGGGAACC CTGGCCTGAA TGGCAGGAGC CGGAGGGCCA AGAAGGTGGC ATCAAGACAT 
TGTGAGGAGC GGCGTCCCCA GAGGGCCAGT GACCAGGCCA GGCCTGGCCC TGAGATCATG 
AGGACCATCC CTGAGGAAGA ACTGACTGAC AACTGGAGAA AAATGAGCTT TGAGATCCTC 
AGGGGCTCTG ACGGGGAAGA CTCAGCCTCA GGTGGGAAGA CTCCAGCTCC GGGCCCTGAG 
GCAGCTTCTG GAGAATGGGA GCTGCTGAGG CTGGATTCCA GCAAGAAGAA GCTGCCCAGC 
CCATGCCCAG ACAAGGAGAG TGACAAGGAC CTTGGTCCTC GGCTCCGGCT CCCCTCAGCC 
CCCGTAGCCA CTGGTCTTTC TACCCTGGAC TCCATCTGTG ACTCCCTGAG TGTTGCTTTC 
CGGGGCATTA GTCACTGTCC TCCTAGTGGG CTCTATGCCC ACCTCTGCCG CTTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCTTATGCC ACTGCTTTCC TTGTCACCGA GTCTGTCTCC 
ATCACCTGTC GCCACCAGCT GCTCACCCAC CTCCACAGAC AGCTCAGCAA GGCCCAGAAG 
CACCGAGGAT CACTTGAAAT AGCAGACCAG CTGCAGGGGC TGAGCCTTCA GGAGATGCCT 
GGAGATGTCC CCCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTT GGAATCTGGC 
CACTTCCCCC AGCCTGAAAA GGAGAGTTTC CAGGAGCGCC TGGCTCTGAT CCCCAGTGGG 
GTGACTGTGT GTGTGTTGGC CCTGGCCACC CTCCAGCCCG GAACCGTGGG CAACACCCTC 
CTGCTGACCC GGCTGGAAAA GGACAGTCCC CCAGTCAGTG TGCAGATTCC CACTGGCCAG 
AACAAGCTTC ATCTGCGTTC AGTCCTGAAT GAGTTTGATG CCATCCAGAA GGCACAGAAA 
GAGAACAGCA GCTGTACTGA CAAGCGAGAA TGGTGGACAG GGCGGCTGGC ACTGGACCAC 
AGGATGGAGG TTCTCATCGC TTCCCTAGAG AAGTCTGTGC TGGGCTGCTG GAAGGGGCTG 
CTGCTGCCGT CCAGTGAGGA GCCCGGCCCT GCCCAGGAGG CCTCCCGCCT ACAGGAGCTG 
CTACAGGACT GTGGCTGGAA ATATCCTGAC CGCACTCTGC TGAAAATCAT GCTCAGTGGT 
GCCGGTGCCC TCACCCCTCA GGACATTCAG GCCCTGGCCT ACGGGCTGTG CCCAACCCAG 
CCAGAGCGAG CCCAGGAGCT CCTGAATGAG GCAGTAGGAC GTCTACAGGG CCTGACAGTA 
CCAAGCAATA GCCACCTTGT CTTGGTCCTA GACAAGGACT TGCAGAAGCT GCCGTGGGAA 
AGCATGCCCA GCCTCCAAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCG CTTCCTACTC 
AGCTACTCCA TCATCAAAGA GTATGGGGCC TCGCCAGTGC TGAGTCAAGG GGTGGATCCA 
CGAAGTACCT TCTATGTCCT GAACCCTCAC AATAACCTGT CAAGCACAGA GGAGCAATTT 
CGAGCCAATT TCAGCAGTGA AGCTGGCTGG AGAGGAGTGG TTGGGGAGGT GCCAAGACCT 
GAACAGGTGC AGGAAGCCCT GACAAAGCAT GATTTGTATA TCTATGCAGG GCATGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCTGTC CTGCGGCTGA GCTGTCGGGC AGTGGCCCTG 
CTGTTTGGCT GTAGCAGTGC GGCCCTGGCT GTGCGTGGAA ACCTGGAGGG GGCTGGCATC 
GTGCTCAAGT ACATCATGGC TGGTTGCCCC TTGTTTCTGG GTAATCTCTG GGATGTGACT 
GACCGCGACA TTGACCGCTA CACGGAAGCT CTGCTGCAAG GCTGGCTTGG AGCAGGCCCA 
GGGGCCCCCC TTCTCTACTA TGTAAACCAG GCCCGCCAAG CTCCCCGACT CAAGTATCTT 
ATTGGGGCTG CACCTATAGC CTATGGCTTG CCTGTCTCTC TGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875742.1
CDS	1185..6572
Translation	MEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_017020253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020253.1

ORF Insert Sequence:

ATGGAGGCAC CATCACCCCC ACTTCGGGCA TTGTATGAGA GCTGCCAGTT CTTCCTTTCA 
GGCCTGGAAC GAGGCACCAA GAGGCGCTAT AGACTTGATG CCATTCTGAG CCTCTTTGCT 
TTTCTTGGAG GGTACTGCTC TCTTCTGCAG CAGCTGCGGG ATGATGGTGT GTATGGGGGC 
TCCTCCAAGC AACAGCAGTC TTTTCTTCAG ATGTACTTTC AGGGACTTCA CCTCTACACT 
GTGGTGGTTT ATGACTTTGC CCAAGGCTGT CAGATAGTTG ATTTGGCTGA CCTGACCCAA 
CTAGTGGACA GTTGTAAATC TACCGTTGTC TGGATGCTGG AGGCCTTAGA GGGCCTGTCG 
GGCCAAGAGC TGACGGACCA CATGGGGATG ACCGCTTCTT ACACCAGTAA TTTGGCCTAC 
AGCTTCTATA GTCACAAGCT CTATGCCGAG GCCTGTGCCA TCTCTGAGCC GCTCTGTCAG 
CACCTGGGTT TGGTGAAGCC AGGCACTTAT CCCGAGGTGC CTCCTGAGAA GTTGCACAGG 
TGCTTCCGGC TACAAGTAGA GAGTTTGAAG AAACTGGGTA AACAGGCCCA GGGCTGCAAG 
ATGGTGATTT TGTGGCTGGC AGCCCTGCAA CCCTGTAGCC CTGAACACAT GGCTGAGCCA 
GTCACTTTCT GGGTTCGGGT CAAGATGGAT GCGGCCAGGG CTGGAGACAA GGAGCTACAG 
CTAAAGACTC TGCGAGACAG CCTCAGTGGC TGGGACCCGG AGACCCTGGC CCTCCTGCTG 
AGGGAGGAGC TGCAGGCCTA CAAGGCGGTG CGGGCCGACA CTGGACAGGA ACGCTTCAAC 
ATCATCTGTG ACCTCCTGGA GCTGAGCCCC GAGGAGACAC CAGCCGGGGC CTGGGCACGA 
GCCACCCACC TGGTAGAACT GGCTCAGGTG CTCTGCTACC ACGACTTTAC GCAGCAGACC 
AACTGCTCTG CTCTGGATGC TATCCGGGAA GCCCTGCAGC TTCTGGACTC TGTGAGGCCT 
GAGGCCCAGG CCAGAGATCA GCTTCTGGAC GATAAAGCAC AGGCCTTGCT GTGGCTTTAC 
ATCTGTACTC TGGAAGCCAA AATGCAGGAA GGTATCGAGC GGGATCGGAG AGCCCAGGCC 
CCTGGTAACT TGGAGGAATT TGAAGTCAAT GACCTGAACT ATGAAGATAA ACTCCAGGAA 
GATCGTTTCC TATACAGTAA CATTGCCTTC AACCTGGCTG CAGATGCTGC TCAGTCCAAA 
TGCCTGGACC AAGCCCTGGC CCTGTGGAAG GAGCTGCTTA CAAAGGGGCA GGCCCCAGCT 
GTACGGTGTC TCCAGCAGAC AGCAGCCTCA CTGCAGATCC TAGCAGCCCT CTACCAGCTG 
GTGGCAAAGC CCATGCAGGC TCTGGAGGTC CTCCTGCTGC TACGGATTGT CTCTGAGAGA 
CTGAAGGACC ACTCGAAGGC AGCTGGCTCC TCCTGCCACA TCACCCAGCT CCTCCTGACC 
CTCGGCTGTC CCAGCTATGC CCAGTTACAC CTGGAAGAGG CAGCATCGAG CCTGAAGCAT 
CTCGATCAGA CTACTGACAC ATACCTGCTC CTTTCCCTGA CCTGTGATCT GCTTCGAAGT 
CAACTCTACT GGACTCACCA GAAGGTGACC AAGGGTGTCT CTCTGCTGCT GTCTGTGCTT 
CGGGATCCTG CCCTCCAGAA GTCCTCCAAG GCTTGGTACT TGCTGCGTGT CCAGGTCCTG 
CAGCTGGTGG CAGCTTACCT TAGCCTCCCG TCAAACAACC TCTCACACTC CCTGTGGGAG 
CAGCTCTGTG CCCAAGGCTG GCAGACACCT GAGATAGCTC TCATAGACTC CCATAAGCTC 
CTCCGAAGCA TCATCCTCCT GCTGATGGGC AGTGACATTC TCTCAACTCA GAAAGCAGCT 
GTGGAGACAT CGTTTTTGGA CTATGGTGAA AATCTGGTAC AAAAATGGCA GGTTCTTTCA 
GAGGTGCTGA GCTGCTCAGA GAAGCTGGTC TGCCACCTGG GCCGCCTGGG TAGTGTGAGT 
GAAGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACAA CAAAGCTGCA GATACCACGC 
CAGTGTGCCC TGTTCCTGGT GCTGAAGGGC GAGCTGGAGC TGGCCCGCAA TGACATTGAT 
CTCTGTCAGT CGGACCTGCA GCAGGTTCTG TTCTTGCTTG AGTCTTGCAC AGAGTTTGGT 
GGGGTGACTC AGCACCTGGA CTCTGTGAAG AAGGTCCACC TGCAGAAGGG GAAGCAGCAG 
GCCCAGGTCC CCTGTCCTCC ACAGCTCCCA GAGGAGGAGC TCTTCCTAAG AGGCCCTGCT 
CTAGAGCTGG TGGCCACTGT GGCCAAGGAG CCTGGCCCCA TAGCACCTTC TACAAACTCC 
TCCCCAGTCT TGAAAACCAA GCCCCAGCCC ATACCCAACT TCCTGTCCCA TTCACCCACC 
TGTGACTGCT CGCTCTGCGC CAGCCCTGTC CTCACAGCAG TCTGTCTGCG CTGGGTATTG 
GTCACGGCAG GGGTGAGGCT GGCCATGGGC CACCAAGCCC AGGGTCTGGA TCTGCTGCAG 
GTCGTGCTGA AGGGCTGTCC TGAAGCCGCT GAGCGCCTCA CCCAAGCTCT CCAAGCTTCC 
CTGAATCATA AAACACCCCC CTCCTTGGTT CCAAGCCTCT TGGATGAGAT CTTGGCTCAA 
GCATACACAC TGTTGGCACT GGAGGGCCTG AACCAGCCAT CAAACGAGAG CCTGCAGAAG 
GTTCTACAGT CAGGGCTGAA GTTTGTAGCA GCACGGATAC CCCACCTAGA GCCCTGGCGA 
GCCAGCCTGC TCTTGATTTG GGCCCTCACA AAACTAGGTG GCCTCAGCTG CTGTACTACC 
CAACTTTTTG CAAGCTCCTG GGGCTGGCAG CCACCATTAA TAAAAAGTGT CCCTGGCTCA 
GAGCCCTCTA AGACTCAGGG CCAAAAACGT TCTGGACGAG GGCGCCAAAA GTTAGCCTCT 
GCTCCCCTGC GCCTCAATAA TACCTCTCAG AAAGGTCTGG AAGGTAGAGG ACTGCCCTGC 
ACACCTAAAC CCCCAGACCG GATCAGGCAA GCTGGCCCTC ATGTCCCCTT CACGGTGTTT 
GAGGAAGTCT GCCCTACAGA GAGCAAGCCT GAAGTACCCC AGGCCCCCAG GGTACAACAG 
AGAGTCCAGA CGCGCCTCAA GGTGAACTTC AGTGATGACA GTGACTTGGA AGACCCTGTC 
TCAGCTGAGG CCTGGCTGGC AGAGGAGCCT AAGAGACGGG GCACTGCTTC CCGGGGCCGG 
GGGCGAGCAA GGAAGGGCCT GAGCCTAAAG ACGGATGCCG TGGTTGCCCC AGGTAGTGCC 
CCTGGGAACC CTGGCCTGAA TGGCAGGAGC CGGAGGGCCA AGAAGGTGGC ATCAAGACAT 
TGTGAGGAGC GGCGTCCCCA GAGGGCCAGT GACCAGGCCA GGCCTGGCCC TGAGATCATG 
AGGACCATCC CTGAGGAAGA ACTGACTGAC AACTGGAGAA AAATGAGCTT TGAGATCCTC 
AGGGGCTCTG ACGGGGAAGA CTCAGCCTCA GGTGGGAAGA CTCCAGCTCC GGGCCCTGAG 
GCAGCTTCTG GAGAATGGGA GCTGCTGAGG CTGGATTCCA GCAAGAAGAA GCTGCCCAGC 
CCATGCCCAG ACAAGGAGAG TGACAAGGAC CTTGGTCCTC GGCTCCGGCT CCCCTCAGCC 
CCCGTAGCCA CTGGTCTTTC TACCCTGGAC TCCATCTGTG ACTCCCTGAG TGTTGCTTTC 
CGGGGCATTA GTCACTGTCC TCCTAGTGGG CTCTATGCCC ACCTCTGCCG CTTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCTTATGCC ACTGCTTTCC TTGTCACCGA GTCTGTCTCC 
ATCACCTGTC GCCACCAGCT GCTCACCCAC CTCCACAGAC AGCTCAGCAA GGCCCAGAAG 
CACCGAGGAT CACTTGAAAT AGCAGACCAG CTGCAGGGGC TGAGCCTTCA GGAGATGCCT 
GGAGATGTCC CCCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTT GGAATCTGGC 
CACTTCCCCC AGCCTGAAAA GGAGAGTTTC CAGGAGCGCC TGGCTCTGAT CCCCAGTGGG 
GTGACTGTGT GTGTGTTGGC CCTGGCCACC CTCCAGCCCG GAACCGTGGG CAACACCCTC 
CTGCTGACCC GGCTGGAAAA GGACAGTCCC CCAGTCAGTG TGCAGATTCC CACTGGCCAG 
AACAAGCTTC ATCTGCGTTC AGTCCTGAAT GAGTTTGATG CCATCCAGAA GGCACAGAAA 
GAGAACAGCA GCTGTACTGA CAAGCGAGAA TGGTGGACAG GGCGGCTGGC ACTGGACCAC 
AGGATGGAGG TTCTCATCGC TTCCCTAGAG AAGTCTGTGC TGGGCTGCTG GAAGGGGCTG 
CTGCTGCCGT CCAGTGAGGA GCCCGGCCCT GCCCAGGAGG CCTCCCGCCT ACAGGAGCTG 
CTACAGGACT GTGGCTGGAA ATATCCTGAC CGCACTCTGC TGAAAATCAT GCTCAGTGGT 
GCCGGTGCCC TCACCCCTCA GGACATTCAG GCCCTGGCCT ACGGGCTGTG CCCAACCCAG 
CCAGAGCGAG CCCAGGAGCT CCTGAATGAG GCAGTAGGAC GTCTACAGGG CCTGACAGTA 
CCAAGCAATA GCCACCTTGT CTTGGTCCTA GACAAGGACT TGCAGAAGCT GCCGTGGGAA 
AGCATGCCCA GCCTCCAAGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCG CTTCCTACTC 
AGCTACTCCA TCATCAAAGA GTATGGGGCC TCGCCAGTGC TGAGTCAAGG GGTGGATCCA 
CGAAGTACCT TCTATGTCCT GAACCCTCAC AATAACCTGT CAAGCACAGA GGAGCAATTT 
CGAGCCAATT TCAGCAGTGA AGCTGGCTGG AGAGGAGTGG TTGGGGAGGT GCCAAGACCT 
GAACAGGTGC AGGAAGCCCT GACAAAGCAT GATTTGTATA TCTATGCAGG GCATGGGGCT 
GGTGCCCGCT TCCTTGATGG GCAGGCTGTC CTGCGGCTGA GCTGTCGGGC AGTGGCCCTG 
CTGTTTGGCT GTAGCAGTGC GGCCCTGGCT GTGCGTGGAA ACCTGGAGGG GGCTGGCATC 
GTGCTCAAGT ACATCATGGC TGGTTGCCCC TTGTTTCTGG GTAATCTCTG GGATGTGACT 
GACCGCGACA TTGACCGCTA CACGGAAGCT CTGCTGCAAG GCTGGCTTGG AGCAGGCCCA 
GGGGCCCCCC TTCTCTACTA TGTAAACCAG GCCCGCCAAG CTCCCCGACT CAAGTATCTT 
ATTGGGGCTG CACCTATAGC CTATGGCTTG CCTGTCTCTC TGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_011539025.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537327.1 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OHu63185
Clone ID Related Accession (Same CDS sequence)	XM_011539025.2
Accession Version	XM_011539025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537327.1
CDS	131..5929
Translation	MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKE

Target ORF information:

RefSeq Version	XM_011539025.2
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539025.2

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( NM_012291.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036423.4 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.

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CloneID	OHu13319
Clone ID Related Accession (Same CDS sequence)	XM_011539024.2 , NM_012291.4 , NM_012291.5 , XM_006719705.3
Accession Version	NM_012291.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	separin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC021072.27 and AC073611.29. On Aug 1, 2019 this sequence version replaced NM_012291.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY455930.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036423.4
CDS	92..6454
Translation	MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	NM_012291.5
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012291.5

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_011539024.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537326.1 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OHu13319
Clone ID Related Accession (Same CDS sequence)	XM_011539024.2 , NM_012291.4 , NM_012291.5 , XM_006719705.3
Accession Version	XM_011539024.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539024.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537326.1
CDS	120..6482
Translation	MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_011539024.2
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539024.2

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_006719705.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719768.1 Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OHu13319
Clone ID Related Accession (Same CDS sequence)	XM_011539024.2 , NM_012291.4 , NM_012291.5 , XM_006719705.3
Accession Version	XM_006719705.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719768.1
CDS	159..6521
Translation	MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_006719705.3
Organism	Homo sapiens(human)
Definition	Homo sapiens extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719705.3

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCAGAA GGAGGCTGAA 
GAGTTGCTGC CCGCCTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTAGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTTGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC ACTCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGCGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAAA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTATAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT GCGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATGTA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGACAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACCACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCT 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCCGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAGCCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA GGAGCTGCAG GCCTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCCGAGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GACTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGTAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTCCTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGAGCCTGA AGCATCTCGA TCAGACTACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACCAAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAACCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CACCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAAG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGTG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA AGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCTGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAACCA GCCATCAAAC 
GAGAGCCTGC AGAAGGTTCT ACAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAACT AGGTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCAGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGTTAG CCTCTGCTCC CCTGCGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTGC CCTGCACACC TAAACCCCCA GACCGGATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAGAGAGCA AGCCTGAAGT ACCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGCCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGCCTGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAATGGCA GGAGCCGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCGGCGT CCCCAGAGGG CCAGTGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAAGAACTGA CTGACAACTG GAGAAAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGACGGG GAAGACTCAG CCTCAGGTGG GAAGACTCCA 
GCTCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGAAGCTGC CCAGCCCATG CCCAGACAAG GAGAGTGACA AGGACCTTGG TCCTCGGCTC 
CGGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC CTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGGCCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GGGGCTGAGC 
CTTCAGGAGA TGCCTGGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGAAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCATCTG CGTTCAGTCC TGAATGAGTT TGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACAGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGACTGTGGC TGGAAATATC CTGACCGCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAGA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGTCTA 
CAGGGCCTGA CAGTACCAAG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAAGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGG ATCCACGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAGGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GATGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCG TGGAAACCTG 
GAGGGGGCTG GCATCGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG CGACATTGAC CGCTACACGG AAGCTCTGCT GCAAGGCTGG 
CTTGGAGCAG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Separase is a marker for prognosis and mitotic activity in breast cancer.
British journal of cancer117(9)1383-1391(2017 Oct)
Gurvits N,L?yttyniemi E,Nyk?nen M,Kuopio T,Kronqvist P,Talvinen K

Separase: Function Beyond Cohesion Cleavage and an Emerging Oncogene.
Journal of cellular biochemistry118(6)1283-1299(2017 Jun)
Kumar R

Proximity mapping of human separase by the BioID approach.
Biochemical and biophysical research communications478(2)656-62(2016 09)
Agircan FG,Hata S,Nussbaum-Krammer C,Atorino E,Schiebel E

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ESPL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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