RAPGEF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF2
Entrez Gene ID	9693
Full Name	Rap guanine nucleotide exchange factor 2
Synonyms	CNrasGEF,NRAPGEP,PDZ-GEF1,PDZGEF1,RA-GEF,RA-GEF-1,Rap-GEP,nRap GEP
General protein information	Preferred Names Rap guanine nucleotide exchange factor 2 Names rap guanine nucleotide exchange factor 2 PDZ domain containing guanine nucleotide exchange factor (GEF) 1 PDZ domain-containing guanine nucleotide exchange factor 1 RA(Ras/Rap1A-associating)-GEF Rap guanine nucleotide exchange factor (GEF) 2 cyclic nucleotide ras GEF neural RAP guanine nucleotide exchange protein ras/Rap1-associating GEF-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.1
Summary	Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609530

mRNA and Protein(s)

mRNA	Protein	Name
XM_006714421.3	XP_006714484.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_006714422.3	XP_006714485.1	rap guanine nucleotide exchange factor 2 isoform X9
NM_014247.2	NP_055062.1	rap guanine nucleotide exchange factor 2 isoform 5
NM_001351724.1	NP_001338653.1	rap guanine nucleotide exchange factor 2 isoform 1
XM_006714420.3	XP_006714483.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_011532425.2	XP_011530727.1	rap guanine nucleotide exchange factor 2 isoform X7
XM_024454286.1	XP_024310054.1	rap guanine nucleotide exchange factor 2 isoform X9
NM_001351728.1	NP_001338657.1	rap guanine nucleotide exchange factor 2 isoform 4
XM_005263359.3	XP_005263416.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_005263358.3	XP_005263415.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_005263359.4	XP_005263416.1	rap guanine nucleotide exchange factor 2 isoform X3
XM_017008857.1	XP_016864346.1	rap guanine nucleotide exchange factor 2 isoform X10
XM_017008856.1	XP_016864345.1	rap guanine nucleotide exchange factor 2 isoform X7
NM_001351728.2	NP_001338657.1	rap guanine nucleotide exchange factor 2 isoform 4
NM_001351724.2	NP_001338653.1	rap guanine nucleotide exchange factor 2 isoform 1
NM_014247.3	NP_055062.1	rap guanine nucleotide exchange factor 2 isoform 5
XM_005263360.3	XP_005263417.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_006714422.4	XP_006714485.1	rap guanine nucleotide exchange factor 2 isoform X8
XM_017008858.1	XP_016864347.1	rap guanine nucleotide exchange factor 2 isoform X10
NM_001351727.1	NP_001338656.1	rap guanine nucleotide exchange factor 2 isoform 3
NM_001351727.2	NP_001338656.1	rap guanine nucleotide exchange factor 2 isoform 3
NM_001351726.1	NP_001338655.1	rap guanine nucleotide exchange factor 2 isoform 2
NM_001351725.1	NP_001338654.1	rap guanine nucleotide exchange factor 2 isoform 2
XM_017008856.2	XP_016864345.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_011532427.2	XP_011530729.1	rap guanine nucleotide exchange factor 2 isoform X11
XM_017008859.1	XP_016864348.1	rap guanine nucleotide exchange factor 2 isoform X12
XM_005263361.3	XP_005263418.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_011532426.2	XP_011530728.1	rap guanine nucleotide exchange factor 2 isoform X8
XM_024454287.1	XP_024310055.1	rap guanine nucleotide exchange factor 2 isoform X9

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAPGEF2	NP_055062.1
	RAPGEF2	XP_001093090.2
Mus musculus (house mouse)	Rapgef2	NP_001093094.2
Rattus norvegicus (Norway rat)	Rapgef2	NP_001101154.1
Gallus gallus (chicken)	RAPGEF2	XP_004940966.1
Xenopus tropicalis (tropical clawed frog)	rapgef2	NP_001120522.1
Pan troglodytes (chimpanzee)	RAPGEF2	XP_001147382.2
Canis lupus familiaris (dog)	RAPGEF2	XP_005629442.1
Danio rerio (zebrafish)	rapgef2	XP_005173396.1
Bos taurus (cattle)	RAPGEF2	NP_001178255.1

Gene Ontology
Bibliography

Related articles in PubMed

Substrate trapping proteomics reveals targets of the ?TrCP2/FBXW11 ubiquitin ligase.
Kim TY, Siesser PF, Rossman KL, Goldfarb D, Mackinnon K, Yan F, Yi X, MacCoss MJ, Moon RT, Der CJ, Major MB
Molecular and cellular biology35(1)167-81(2015 Jan)

Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex.
Ye T, Ip JP, Fu AK, Ip NY
Nature communications54826(2014 Sep)

The PDZ domain of the guanine nucleotide exchange factor PDZGEF directs binding to phosphatidic acid during brush border formation.
Consonni SV, Brouwer PM, van Slobbe ES, Bos JL
PloS one9(5)e98253(2014)

Control of epithelial cell migration and invasion by the IKK?- and CK1?-mediated degradation of RAPGEF2.
Magliozzi R, Low TY, Weijts BG, Cheng T, Spanjaard E, Mohammed S, van Veen A, Ovaa H, de Rooij J, Zwartkruis FJ, Bos JL, de Bruin A, Heck AJ, Guardavaccaro D
Developmental cell27(5)574-85(2013 Dec)

NEDD4-1 regulates migration and invasion of glioma cells through CNrasGEF ubiquitination in vitro.
Zhang H, Nie W, Zhang X, Zhang G, Li Z, Wu H, Shi Q, Chen Y, Ding Z, Zhou X, Yu R
PloS one8(12)e82789(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SCF(FBXW11) bound, polyubiquitylated, and destabilized RAPGEF2, a guanine nucleotide exchange factor that activates the small GTPase RAP1
Title: Substrate trapping proteomics reveals targets of the ?TrCP2/FBXW11 ubiquitin ligase.

Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex.
Title: Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex.

local accumulation of PDZGEF at the apical membrane during establishment of epithelial polarity is mediated by electrostatic interactions between positively charged side chains in the PDZ domain and negatively charged phosphatidic acid.
Title: The PDZ domain of the guanine nucleotide exchange factor PDZGEF directs binding to phosphatidic acid during brush border formation.

We report that in response to factors that promote cell motility, the Rap guanine exchange factor RAPGEF2 is rapidly phosphorylated by I-kappa-B-kinase-beta and casein kinase-1alpha and consequently degraded by the proteasome.
Title: Control of epithelial cell migration and invasion by the IKK?- and CK1?-mediated degradation of RAPGEF2.

NEDD4-1 regulates cell migration and invasion through ubiquitination of CNrasGEF in vitro.
Title: NEDD4-1 regulates migration and invasion of glioma cells through CNrasGEF ubiquitination in vitro.

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09032	NM_014247.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu95558	NM_001351726.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95558	NM_001351725.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95559	NM_001351727.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu105605	XM_024454286.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu105605	XM_024454287.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95557	XM_017008856.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95558	XM_017008858.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95558	XM_017008857.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95559	XM_017008859.1 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu39237	NM_001351724.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu63163	NM_001351728.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu39234	XM_005263358.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39235	XM_006714420.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39236	XM_005263359.4 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39237	XM_006714421.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39238	XM_005263360.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu39239	XM_005263361.3 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu39240	XM_006714422.4 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu39240	XM_011532426.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu63162	XM_011532425.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu63163	XM_011532427.2 Latest version!	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu39236	XM_005263359.3	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu63163	NM_001351728.1	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu39237	NM_001351724.1	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu09032	NM_014247.2	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu39240	XM_006714422.3	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu95559	NM_001351727.1	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu95557	XM_017008856.1	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF2 ( NM_014247.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055062.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu09032
Clone ID Related Accession (Same CDS sequence)	NM_014247.2 , NM_014247.3
Accession Version	NM_014247.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA188103.1, AB002311.1, BU620827.1, BC110355.1 and BP386101.1. On or before Jul 25, 2007 this sequence version replaced XM_934717.2, XM_934718.2, XM_376350.4, XM_934714.2, XM_944412.2, XM_944410.2, XM_944403.2, NM_014247.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002311.1, SRR1803613.248869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055062.1
CDS	311..4810
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_014247.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014247.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_014247.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055062.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

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CloneID	OHu09032
Clone ID Related Accession (Same CDS sequence)	NM_014247.2 , NM_014247.3
Accession Version	NM_014247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5, DA188103.1, AB002311.1, BU620827.1, BC110355.1, BP386101.1 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_014247.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002311.1, SRR1803613.248869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055062.1
CDS	348..4847
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_014247.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014247.3

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351726.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338655.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu95558
Clone ID Related Accession (Same CDS sequence)	XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
Accession Version	NM_001351726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008858.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.26208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338655.1
CDS	183..4817
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351726.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351726.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351725.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338654.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu95558
Clone ID Related Accession (Same CDS sequence)	XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
Accession Version	NM_001351725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008857.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.104810.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338654.1
CDS	1116..5750
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351725.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351725.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351727.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338656.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

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CloneID	OHu95559
Clone ID Related Accession (Same CDS sequence)	NM_001351727.1 , NM_001351727.2 , XM_017008859.1
Accession Version	NM_001351727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_017008859.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.73881.1, SRR1803617.77819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338656.1
CDS	478..5052
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351727.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351727.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351727.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338656.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

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CloneID	OHu95559
Clone ID Related Accession (Same CDS sequence)	NM_001351727.1 , NM_001351727.2 , XM_017008859.1
Accession Version	NM_001351727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_001351727.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.77819.1, SRR1803617.73881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338656.1
CDS	348..4922
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351727.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351727.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024454286.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310054.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

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CloneID	OHu105605
Clone ID Related Accession (Same CDS sequence)	XM_024454286.1 , XM_024454287.1
Accession Version	XM_024454286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT 
CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG 
ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT 
GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG 
GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC 
CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT 
CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG 
CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG 
GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT 
GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC 
ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA 
AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC 
TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT 
ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC 
ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT 
GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA 
GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG 
AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT 
GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT 
ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA 
GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC 
AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA 
CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT 
CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC 
GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA 
AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC 
AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT 
TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC 
TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG 
CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT 
TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA 
CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA 
CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310054.1
CDS	2168..6907
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024454286.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454286.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT 
CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG 
ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT 
GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG 
GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC 
CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT 
CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG 
CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG 
GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT 
GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC 
ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA 
AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC 
TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT 
ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC 
ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT 
GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA 
GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG 
AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT 
GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT 
ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA 
GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC 
AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA 
CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT 
CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC 
GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA 
AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC 
AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT 
TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC 
TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG 
CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT 
TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA 
CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA 
CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_024454287.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024310055.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu105605
Clone ID Related Accession (Same CDS sequence)	XM_024454286.1 , XM_024454287.1
Accession Version	XM_024454287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT 
CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG 
ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT 
GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG 
GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC 
CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT 
CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG 
CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG 
GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT 
GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC 
ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA 
AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC 
TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT 
ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC 
ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT 
GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA 
GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG 
AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT 
GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT 
ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA 
GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC 
AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA 
CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT 
CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC 
GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA 
AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC 
AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT 
TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC 
TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG 
CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT 
TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA 
CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA 
CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024310055.1
CDS	1210..5949
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_024454287.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454287.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGCTT 
CCTGCAGATT TCACAAAACT GCATCTTACT GACAGTCTCC ACCCACAGGT GACCCACGTT 
TCTTCTAGCC ATTCAGGATG TAGTATCACT AGTGATTCTG GGAGCAGCAG TCTTTCTGAT 
ATCTACCAGG CCACAGAAAG CGAGGCTGGT GATATGGACC TGAGTGGGTT GCCAGAAACA 
GCAGTGGATT CCGAAGACGA CGACGATGAA GAAGACATTG AGAGAGCATC AGATCCTCTG 
ATGAGCAGGG ACATTGTGAG AGACTGCCTA GAGAAGGACC CAATTGACCG GACAGATGAT 
GACATTGAAC AACTCTTGGA ATTTATGCAC CAGTTGCCTG CTTTTGCCAA TATGACAATG 
TCAGTGAGGC GAGAACTCTG TGCTGTGATG GTGTTCGCAG TGGTGGAAAG AGCAGGGACC 
ATAGTGTTAA ATGATGGTGA AGAGCTGGAC TCCTGGTCAG TGATTCTCAA TGGATCTGTG 
GAAGTGACTT ATCCAGATGG AAAAGCAGAA ATACTGTGCA TGGGAAATAG TTTTGGTGTC 
TCTCCTACCA TGGACAAAGA ATACATGAAA GGAGTGATGA GAACAAAGGT GGATGACTGC 
CAGTTTGTCT GCATAGCCCA GCAAGATTAC TGCCGTATTC TCAATCAAGT AGAAAAGAAC 
ATGCAAAAAG TTGAAGAGGA AGGAGAGATT GTTATGGTGA AAGAACACCG AGAACTTGAT 
CGAACTGGAA CAAGAAAGGG ACACATTGTC ATCAAGGGTA CCTCAGAAAG GTTAACAATG 
CATTTGGTGG AAGAGCATTC AGTAGTAGAT CCAACATTCA TAGAAGACTT TCTGTTGACC 
TATAGGACTT TTCTTTCTAG CCCAATGGAA GTGGGCAAAA AGTTATTGGA GTGGTTTAAT 
GACCCGAGCC TCAGGGATAA GGTTACACGG GTAGTATTAT TGTGGGTAAA TAATCACTTC 
AATGACTTTG AAGGAGATCC TGCAATGACT CGATTTTTAG AAGAATTTGA AAACAATCTG 
GAAAGAGAGA AAATGGGTGG ACACCTAAGG CTGTTGAATA TCGCGTGTGC TGCTAAAGCA 
AAAAGAAGAT TGATGACGTT AACAAAACCA TCCCGAGAAG CTCCTTTGCC TTTTATCTTA 
CTTGGAGGCT CTGAGAAGGG ATTTGGAATC TTTGTTGACA GTGTAGATTC AGGTAGCAAA 
GCAACTGAAG CAGGCTTGAA ACGGGGGGAT CAGATATTAG AAGTAAATGG CCAAAACTTT 
GAAAACATTC AGCTGTCAAA AGCTATGGAA ATTCTTAGAA ATAACACACA TTTATCTATC 
ACTGTGAAAA CCAATTTATT TGTATTTAAA GAACTTCTAA CAAGATTGTC AGAAGAGAAA 
AGAAATGGTG CCCCCCACCT TCCTAAAATT GGTGACATTA AAAAGGCCAG TCGCTACTCC 
ATTCCAGATC TTGCTGTAGA TGTAGAACAG GTGATAGGAC TTGAAAAAGT GAACAAAAAA 
AGTAAAGCCA ACACTGTGGG AGGAAGGAAC AAGCTGAAAA AGATACTCGA CAAGACTCGG 
ATCAGTATCT TGCCACAGAA ACCATACAAT GATATTGGGA TTGGTCAGTC TCAAGATGAC 
AGCATAGTAG GATTAAGGCA GACAAAGCAC ATCCCAACTG CATTGCCTGT CAGTGGAACC 
TTATCATCCA GTAATCCTGA TTTATTGCAG TCACATCATC GCATTTTAGA CTTCAGTGCT 
ACTCCTGACT TGCCAGATCA AGTGCTAAGG GTTTTTAAGG CTGATCAGCA AAGCCGCTAC 
ATCATGATCA GTAAGGACAC TACAGCAAAG GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT 
GCTGTTACTG CCACCCCGGA TCAATATTCA CTATGTGAGG TCTCTGTCAC ACCTGAGGGA 
GTAATCAAAC AAAGAAGACT TCCAGATCAG CTTTCCAAAC TTGCAGACAG AATACAACTG 
AGTGGAAGGT ATTATCTGAA AAACAACATG GAAACAGAAA CTCTTTGTTC AGATGAAGAT 
GCTCAGGAGT TGTTGAGAGA GAGTCAAATT TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT 
GCAACACAGC TCTCTATGCG AAATTTTGAA CTCTTTCGCA ACATTGAACC TACTGAATAT 
ATAGATGATT TATTTAAACT CAGATCAAAA ACCAGCTGTG CCAACCTGAA GAGATTTGAA 
GAAGTCATTA ACCAGGAAAC ATTTTGGGTA GCATCTGAAA TTCTCAGAGA AACAAACCAG 
CTGAAGAGGA TGAAGATCAT TAAGCATTTC ATCAAGATAG CACTGCACTG TAGGGAATGC 
AAGAATTTTA ACTCAATGTT TGCAATCATC AGTGGCCTAA ACCTGGCACC AGTGGCAAGA 
CTGCGAACGA CCTGGGAGAA ACTTCCCAAT AAATACGAAA AACTATTTCA AGATCTCCAA 
GACCTGTTTG ATCCTTCCAG AAACATGGCA AAATATCGTA ATGTTCTCAA TAGTCAAAAT 
CTACAACCTC CCATAATCCC TCTATTCCCA GTTATCAAAA AGGATCTCAC CTTCCTTCAC 
GAAGGAAATG ACTCAAAAGT AGACGGGCTG GTCAATTTTG AGAAGCTAAG GATGATTGCA 
AAAGAAATTC GTCACGTTGG CCGAATGGCT TCAGTGAACA TGGACCCTGC CCTCATGTTC 
AGGACTCGGA AGAAGAAATG GCGGAGTTTG GGGTCTCTCA GCCAGGGTAG TACAAATGCA 
ACAGTGCTAG ATGTTGCTCA GACAGGTGGT CATAAAAAGC GGGTACGTCG TAGTTCCTTT 
CTCAATGCCA AAAAGCTTTA TGAAGATGCC CAAATGGCTC GAAAAGTGAA GCAGTACCTT 
TCCAATTTGG AGCTAGAAAT GGACGAGGAG AGTCTTCAGA CATTATCTCT GCAGTGTGAG 
CCAGCAACCA ACACATTGCC TAAGAATCCT GGTGACAAAA AGCCTGTCAA ATCCGAGACC 
TCTCCAGTAG CTCCAAGGGC AGGGTCACAA CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG 
CAGCAGCCAC CACCAGCACA TAAAATCAAC CAGGGACTAC AGGTTCCCGC CGTGTCCCTT 
TATCCTTCAC GGAAGAAAGT GCCCGTAAAG GATCTCCCAC CTTTTGGCAT AAACTCTCCA 
CAAGCTTTAA AAAAAATTCT TTCTTTGTCT GAAGAAGGAA GTTTGGAACG TCACAAGAAA 
CAGGCTGAAG ATACAATATC AAATGCATCT TCGCAGCTTT CTTCTCCTCC TACTTCTCCA 
CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC AATCAGCTGA GATCTTTTGG CTCCGGGCAG 
TTGGACTTAA CCAGTTCCTC CTCTTCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
GCACCACGGA CCTATGGGAT AGGCTATACT TTGGCTCCCA GTGGTACTGT GGATAATTTT 
TCAGATTCTG GTCACAGTGA AATTTCTTCA CGATCCAGTA TTGTTAGCAA TTCGTCTTTT 
GACTCAGTGC CAGTCTCACT GCACGATGAG AGGCGCCAGA GGCATTCTGT CAGCATCGTG 
GAAACAAACC TAGGGATGGG CAGGATGGAG AGGCGGACCA TGATTGAACC TGATCAGTAT 
AGCTTGGGGT CCTATGCACC AATGTCCGAG GGCCGAGGCT TATATGCTAC AGCTACAGTA 
ATTTCTTCTC CAAGCACAGA GGAACTTTCC CAGGATCAGG GGGATCGCGC GTCACTTGAT 
GCTGCTGACA GTGGCCGTGG GAGCTGGACG TCATGCTCAA GTGGCTCCCA TGATAATATA 
CAGACGATCC AGCACCAGAG AAGCTGGGAG ACTCTTCCAT TCGGGCATAC TCACTTTGAT 
TATTCAGGGG ATCCTGCAGG TTTATGGGCA TCAAGCAGCC ATATGGACCA AATTATGTTT 
TCTGATCATA GCACAAAGTA TAACAGGCAA AATCAAAGTA GAGAGAGCCT TGAACAAGCC 
CAGTCCCGAG CAAGCTGGGC GTCTTCCACA GGTTACTGGG GAGAAGACTC AGAAGGTGAC 
ACAGGCACAA TAAAGCGGAG GGGTGGAAAG GATGTTTCCA TTGAAGCCGA AAGCAGTAGC 
CTAACGTCTG TGACTACGGA AGAAACCAAG CCTGTCCCCA TGCCTGCCCA CATAGCTGTG 
GCATCAAGTA CTACAAAGGG GCTCATTGCA CGAAAGGAGG GCAGGTATCG AGAGCCCCCG 
CCCACCCCTC CCGGCTACAT TGGAATTCCC ATTACTGACT TTCCAGAAGG GCACTCCCAT 
CCAGCCAGGA AACCGCCGGA CTACAACGTG GCCCTTCAGA GATCGCGGAT GGTCGCACGA 
TCCTCCGACA CAGCTGGGCC TTCATCCGTA CAGCAGCCAC ATGGGCATCC CACCAGCAGC 
AGGCCTGTGA ACAAACCTCA GTGGCATAAA CCGAACGAGT CTGACCCGCG CCTCGCCCCC 
TATCAGTCCC AAGGGTTTTC CACCGAGGAG GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_017008856.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864345.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu95557
Clone ID Related Accession (Same CDS sequence)	XM_017008856.1 , XM_017008856.2
Accession Version	XM_017008856.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG 
CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC 
AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA 
ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG 
AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA 
AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG 
TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG 
GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA 
GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG 
CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT 
GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC 
TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT 
TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864345.1
CDS	96..5054
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_017008856.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008856.1

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG 
CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC 
AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA 
ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG 
AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA 
AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG 
TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG 
GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA 
GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG 
CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT 
GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC 
TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT 
TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_017008856.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864345.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

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CloneID	OHu95557
Clone ID Related Accession (Same CDS sequence)	XM_017008856.1 , XM_017008856.2
Accession Version	XM_017008856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG 
CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC 
AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA 
ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG 
AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA 
AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG 
TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG 
GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA 
GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG 
CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT 
GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC 
TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT 
TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864345.1
CDS	95..5053
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_017008856.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008856.2

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGCTATACTT TGGCTCCCAG TGGTACTGTG GATAATTTTT CAGATTCTGG TCACAGTGAA 
ATTTCTTCAC GATCCAGTAT TGTTAGCAAT TCGTCTTTTG ACTCAGTGCC AGTCTCACTG 
CACGATGAGA GGCGCCAGAG GCATTCTGTC AGCATCGTGG AAACAAACCT AGGGATGGGC 
AGGATGGAGA GGCGGACCAT GATTGAACCT GATCAGTATA GCTTGGGGTC CTATGCACCA 
ATGTCCGAGG GCCGAGGCTT ATATGCTACA GCTACAGTAA TTTCTTCTCC AAGCACAGAG 
GAACTTTCCC AGGATCAGGG GGATCGCGCG TCACTTGATG CTGCTGACAG TGGCCGTGGG 
AGCTGGACGT CATGCTCAAG TGGCTCCCAT GATAATATAC AGACGATCCA GCACCAGAGA 
AGCTGGGAGA CTCTTCCATT CGGGCATACT CACTTTGATT ATTCAGGGGA TCCTGCAGGT 
TTATGGGCAT CAAGCAGCCA TATGGACCAA ATTATGTTTT CTGATCATAG CACAAAGTAT 
AACAGGCAAA ATCAAAGTAG AGAGAGCCTT GAACAAGCCC AGTCCCGAGC AAGCTGGGCG 
TCTTCCACAG GTTACTGGGG AGAAGACTCA GAAGGTGACA CAGGCACAAT AAAGCGGAGG 
GGTGGAAAGG ATGTTTCCAT TGAAGCCGAA AGCAGTAGCC TAACGTCTGT GACTACGGAA 
GAAACCAAGC CTGTCCCCAT GCCTGCCCAC ATAGCTGTGG CATCAAGTAC TACAAAGGGG 
CTCATTGCAC GAAAGGAGGG CAGGTATCGA GAGCCCCCGC CCACCCCTCC CGGCTACATT 
GGAATTCCCA TTACTGACTT TCCAGAAGGG CACTCCCATC CAGCCAGGAA ACCGCCGGAC 
TACAACGTGG CCCTTCAGAG ATCGCGGATG GTCGCACGAT CCTCCGACAC AGCTGGGCCT 
TCATCCGTAC AGCAGCCACA TGGGCATCCC ACCAGCAGCA GGCCTGTGAA CAAACCTCAG 
TGGCATAAAC CGAACGAGTC TGACCCGCGC CTCGCCCCCT ATCAGTCCCA AGGGTTTTCC 
ACCGAGGAGG ATGAAGATGA ACAAGTTTCT GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_017008857.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864346.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

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CloneID	OHu95558
Clone ID Related Accession (Same CDS sequence)	XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
Accession Version	XM_017008857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864346.1
CDS	1209..5843
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_017008857.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008857.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_017008858.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864347.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

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CloneID	OHu95558
Clone ID Related Accession (Same CDS sequence)	XM_017008857.1 , XM_017008858.1 , NM_001351726.1 , NM_001351725.1
Accession Version	XM_017008858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	rap guanine nucleotide exchange factor 2 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864347.1
CDS	177..4811
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_017008858.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008858.1

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_017008859.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864348.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.

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CloneID	OHu95559
Clone ID Related Accession (Same CDS sequence)	NM_001351727.1 , NM_001351727.2 , XM_017008859.1
Accession Version	XM_017008859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	rap guanine nucleotide exchange factor 2 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864348.1
CDS	995..5569
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_017008859.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008859.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGCT ATACTTTGGC TCCCAGTGGT 
ACTGTGGATA ATTTTTCAGA TTCTGGTCAC AGTGAAATTT CTTCACGATC CAGTATTGTT 
AGCAATTCGT CTTTTGACTC AGTGCCAGTC TCACTGCACG ATGAGAGGCG CCAGAGGCAT 
TCTGTCAGCA TCGTGGAAAC AAACCTAGGG ATGGGCAGGA TGGAGAGGCG GACCATGATT 
GAACCTGATC AGTATAGCTT GGGGTCCTAT GCACCAATGT CCGAGGGCCG AGGCTTATAT 
GCTACAGCTA CAGTAATTTC TTCTCCAAGC ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT 
CGCGCGTCAC TTGATGCTGC TGACAGTGGC CGTGGGAGCT GGACGTCATG CTCAAGTGGC 
TCCCATGATA ATATACAGAC GATCCAGCAC CAGAGAAGCT GGGAGACTCT TCCATTCGGG 
CATACTCACT TTGATTATTC AGGGGATCCT GCAGGTTTAT GGGCATCAAG CAGCCATATG 
GACCAAATTA TGTTTTCTGA TCATAGCACA AAGTATAACA GGCAAAATCA AAGTAGAGAG 
AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC TGGGCGTCTT CCACAGGTTA CTGGGGAGAA 
GACTCAGAAG GTGACACAGG CACAATAAAG CGGAGGGGTG GAAAGGATGT TTCCATTGAA 
GCCGAAAGCA GTAGCCTAAC GTCTGTGACT ACGGAAGAAA CCAAGCCTGT CCCCATGCCT 
GCCCACATAG CTGTGGCATC AAGTACTACA AAGGGGCTCA TTGCACGAAA GGAGGGCAGG 
TATCGAGAGC CCCCGCCCAC CCCTCCCGGC TACATTGGAA TTCCCATTAC TGACTTTCCA 
GAAGGGCACT CCCATCCAGC CAGGAAACCG CCGGACTACA ACGTGGCCCT TCAGAGATCG 
CGGATGGTCG CACGATCCTC CGACACAGCT GGGCCTTCAT CCGTACAGCA GCCACATGGG 
CATCCCACCA GCAGCAGGCC TGTGAACAAA CCTCAGTGGC ATAAACCGAA CGAGTCTGAC 
CCGCGCCTCG CCCCCTATCA GTCCCAAGGG TTTTCCACCG AGGAGGATGA AGATGAACAA 
GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351724.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338653.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39237
Clone ID Related Accession (Same CDS sequence)	XM_006714421.3 , NM_001351724.1 , NM_001351724.2
Accession Version	NM_001351724.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095064.3, AC105316.5 and AC074344.5. This sequence is a reference standard in the RefSeqGene project. On May 24, 2017 this sequence version replaced XM_006714421.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.175373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338653.1
CDS	38..5095
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351724.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351724.1

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351724.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338653.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

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CloneID	OHu39237
Clone ID Related Accession (Same CDS sequence)	XM_006714421.3 , NM_001351724.1 , NM_001351724.2
Accession Version	NM_001351724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC095064.3, AC105316.5 and AC074344.5. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001351724.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.175373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338653.1
CDS	113..5170
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351724.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351724.2

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351728.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338657.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

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CloneID	OHu63163
Clone ID Related Accession (Same CDS sequence)	NM_001351728.1 , NM_001351728.2 , XM_011532427.2
Accession Version	NM_001351728.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-03
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On May 24, 2017 this sequence version replaced XM_011532427.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.113940.1, SRR1803611.140636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338657.1
CDS	478..5100
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351728.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351728.1

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( NM_001351728.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338657.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

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CloneID	OHu63163
Clone ID Related Accession (Same CDS sequence)	NM_001351728.1 , NM_001351728.2 , XM_011532427.2
Accession Version	NM_001351728.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105316.5 and AC074344.5. On Jun 2, 2019 this sequence version replaced NM_001351728.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.140636.1, SRR1803615.113940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338657.1
CDS	348..4970
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	NM_001351728.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351728.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005263358.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263415.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OHu39234
Clone ID Related Accession (Same CDS sequence)	XM_005263358.3
Accession Version	XM_005263358.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263358.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GTGAGAACAG TAACAAGAAT 
AACAATGCAC CACGGACCTA TGGGATAGGC TATACTTTGG CTCCCAGTGG TACTGTGGAT 
AATTTTTCAG ATTCTGGTCA CAGTGAAATT TCTTCACGAT CCAGTATTGT TAGCAATTCG 
TCTTTTGACT CAGTGCCAGT CTCACTGCAC GATGAGAGGC GCCAGAGGCA TTCTGTCAGC 
ATCGTGGAAA CAAACCTAGG GATGGGCAGG ATGGAGAGGC GGACCATGAT TGAACCTGAT 
CAGTATAGCT TGGGGTCCTA TGCACCAATG TCCGAGGGCC GAGGCTTATA TGCTACAGCT 
ACAGTAATTT CTTCTCCAAG CACAGAGGAA CTTTCCCAGG ATCAGGGGGA TCGCGCGTCA 
CTTGATGCTG CTGACAGTGG CCGTGGGAGC TGGACGTCAT GCTCAAGTGG CTCCCATGAT 
AATATACAGA CGATCCAGCA CCAGAGAAGC TGGGAGACTC TTCCATTCGG GCATACTCAC 
TTTGATTATT CAGGGGATCC TGCAGGTTTA TGGGCATCAA GCAGCCATAT GGACCAAATT 
ATGTTTTCTG ATCATAGCAC AAAGTATAAC AGGCAAAATC AAAGTAGAGA GAGCCTTGAA 
CAAGCCCAGT CCCGAGCAAG CTGGGCGTCT TCCACAGGTT ACTGGGGAGA AGACTCAGAA 
GGTGACACAG GCACAATAAA GCGGAGGGGT GGAAAGGATG TTTCCATTGA AGCCGAAAGC 
AGTAGCCTAA CGTCTGTGAC TACGGAAGAA ACCAAGCCTG TCCCCATGCC TGCCCACATA 
GCTGTGGCAT CAAGTACTAC AAAGGGGCTC ATTGCACGAA AGGAGGGCAG GTATCGAGAG 
CCCCCGCCCA CCCCTCCCGG CTACATTGGA ATTCCCATTA CTGACTTTCC AGAAGGGCAC 
TCCCATCCAG CCAGGAAACC GCCGGACTAC AACGTGGCCC TTCAGAGATC GCGGATGGTC 
GCACGATCCT CCGACACAGC TGGGCCTTCA TCCGTACAGC AGCCACATGG GCATCCCACC 
AGCAGCAGGC CTGTGAACAA ACCTCAGTGG CATAAACCGA ACGAGTCTGA CCCGCGCCTC 
GCCCCCTATC AGTCCCAAGG GTTTTCCACC GAGGAGGATG AAGATGAACA AGTTTCTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263415.1
CDS	95..5200
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005263358.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263358.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GTGAGAACAG TAACAAGAAT 
AACAATGCAC CACGGACCTA TGGGATAGGC TATACTTTGG CTCCCAGTGG TACTGTGGAT 
AATTTTTCAG ATTCTGGTCA CAGTGAAATT TCTTCACGAT CCAGTATTGT TAGCAATTCG 
TCTTTTGACT CAGTGCCAGT CTCACTGCAC GATGAGAGGC GCCAGAGGCA TTCTGTCAGC 
ATCGTGGAAA CAAACCTAGG GATGGGCAGG ATGGAGAGGC GGACCATGAT TGAACCTGAT 
CAGTATAGCT TGGGGTCCTA TGCACCAATG TCCGAGGGCC GAGGCTTATA TGCTACAGCT 
ACAGTAATTT CTTCTCCAAG CACAGAGGAA CTTTCCCAGG ATCAGGGGGA TCGCGCGTCA 
CTTGATGCTG CTGACAGTGG CCGTGGGAGC TGGACGTCAT GCTCAAGTGG CTCCCATGAT 
AATATACAGA CGATCCAGCA CCAGAGAAGC TGGGAGACTC TTCCATTCGG GCATACTCAC 
TTTGATTATT CAGGGGATCC TGCAGGTTTA TGGGCATCAA GCAGCCATAT GGACCAAATT 
ATGTTTTCTG ATCATAGCAC AAAGTATAAC AGGCAAAATC AAAGTAGAGA GAGCCTTGAA 
CAAGCCCAGT CCCGAGCAAG CTGGGCGTCT TCCACAGGTT ACTGGGGAGA AGACTCAGAA 
GGTGACACAG GCACAATAAA GCGGAGGGGT GGAAAGGATG TTTCCATTGA AGCCGAAAGC 
AGTAGCCTAA CGTCTGTGAC TACGGAAGAA ACCAAGCCTG TCCCCATGCC TGCCCACATA 
GCTGTGGCAT CAAGTACTAC AAAGGGGCTC ATTGCACGAA AGGAGGGCAG GTATCGAGAG 
CCCCCGCCCA CCCCTCCCGG CTACATTGGA ATTCCCATTA CTGACTTTCC AGAAGGGCAC 
TCCCATCCAG CCAGGAAACC GCCGGACTAC AACGTGGCCC TTCAGAGATC GCGGATGGTC 
GCACGATCCT CCGACACAGC TGGGCCTTCA TCCGTACAGC AGCCACATGG GCATCCCACC 
AGCAGCAGGC CTGTGAACAA ACCTCAGTGG CATAAACCGA ACGAGTCTGA CCCGCGCCTC 
GCCCCCTATC AGTCCCAAGG GTTTTCCACC GAGGAGGATG AAGATGAACA AGTTTCTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_006714420.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714483.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OHu39235
Clone ID Related Accession (Same CDS sequence)	XM_006714420.3
Accession Version	XM_006714420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC 
ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT 
TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC 
GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC 
ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA 
CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA 
GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT 
GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG 
GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA 
AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA 
GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT 
CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG 
ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT 
GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA 
GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG 
CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC 
CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT 
GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC 
ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT 
AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG 
CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT 
AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC 
ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA 
AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT 
TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG 
TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC 
TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA 
TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC 
CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC 
TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC 
ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC 
TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT 
CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT 
GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA 
AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG 
CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC 
ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT 
CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA 
CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG 
AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA 
AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT 
ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA 
AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA TCTTTTGGCT CCGGGCAGTT GGACTTAACC 
AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC AGTAACAAGA ATAACAATGC ACCACGGACC 
TATGGGATAG GCTATACTTT GGCTCCCAGT GGTACTGTGG ATAATTTTTC AGATTCTGGT 
CACAGTGAAA TTTCTTCACG ATCCAGTATT GTTAGCAATT CGTCTTTTGA CTCAGTGCCA 
GTCTCACTGC ACGATGAGAG GCGCCAGAGG CATTCTGTCA GCATCGTGGA AACAAACCTA 
GGGATGGGCA GGATGGAGAG GCGGACCATG ATTGAACCTG ATCAGTATAG CTTGGGGTCC 
TATGCACCAA TGTCCGAGGG CCGAGGCTTA TATGCTACAG CTACAGTAAT TTCTTCTCCA 
AGCACAGAGG AACTTTCCCA GGATCAGGGG GATCGCGCGT CACTTGATGC TGCTGACAGT 
GGCCGTGGGA GCTGGACGTC ATGCTCAAGT GGCTCCCATG ATAATATACA GACGATCCAG 
CACCAGAGAA GCTGGGAGAC TCTTCCATTC GGGCATACTC ACTTTGATTA TTCAGGGGAT 
CCTGCAGGTT TATGGGCATC AAGCAGCCAT ATGGACCAAA TTATGTTTTC TGATCATAGC 
ACAAAGTATA ACAGGCAAAA TCAAAGTAGA GAGAGCCTTG AACAAGCCCA GTCCCGAGCA 
AGCTGGGCGT CTTCCACAGG TTACTGGGGA GAAGACTCAG AAGGTGACAC AGGCACAATA 
AAGCGGAGGG GTGGAAAGGA TGTTTCCATT GAAGCCGAAA GCAGTAGCCT AACGTCTGTG 
ACTACGGAAG AAACCAAGCC TGTCCCCATG CCTGCCCACA TAGCTGTGGC ATCAAGTACT 
ACAAAGGGGC TCATTGCACG AAAGGAGGGC AGGTATCGAG AGCCCCCGCC CACCCCTCCC 
GGCTACATTG GAATTCCCAT TACTGACTTT CCAGAAGGGC ACTCCCATCC AGCCAGGAAA 
CCGCCGGACT ACAACGTGGC CCTTCAGAGA TCGCGGATGG TCGCACGATC CTCCGACACA 
GCTGGGCCTT CATCCGTACA GCAGCCACAT GGGCATCCCA CCAGCAGCAG GCCTGTGAAC 
AAACCTCAGT GGCATAAACC GAACGAGTCT GACCCGCGCC TCGCCCCCTA TCAGTCCCAA 
GGGTTTTCCA CCGAGGAGGA TGAAGATGAA CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714483.1
CDS	95..5182
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_006714420.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714420.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC 
ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT 
TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC 
GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC 
ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA 
CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA 
GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT 
GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG 
GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA 
AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA 
GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT 
CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG 
ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT 
GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA 
GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG 
CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC 
CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT 
GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC 
ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT 
AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG 
CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT 
AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC 
ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA 
AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT 
TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG 
TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC 
TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA 
TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC 
CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC 
TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC 
ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC 
TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT 
CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT 
GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA 
AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG 
CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC 
ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT 
CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA 
CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG 
AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA 
AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT 
ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA 
AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA TCTTTTGGCT CCGGGCAGTT GGACTTAACC 
AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC AGTAACAAGA ATAACAATGC ACCACGGACC 
TATGGGATAG GCTATACTTT GGCTCCCAGT GGTACTGTGG ATAATTTTTC AGATTCTGGT 
CACAGTGAAA TTTCTTCACG ATCCAGTATT GTTAGCAATT CGTCTTTTGA CTCAGTGCCA 
GTCTCACTGC ACGATGAGAG GCGCCAGAGG CATTCTGTCA GCATCGTGGA AACAAACCTA 
GGGATGGGCA GGATGGAGAG GCGGACCATG ATTGAACCTG ATCAGTATAG CTTGGGGTCC 
TATGCACCAA TGTCCGAGGG CCGAGGCTTA TATGCTACAG CTACAGTAAT TTCTTCTCCA 
AGCACAGAGG AACTTTCCCA GGATCAGGGG GATCGCGCGT CACTTGATGC TGCTGACAGT 
GGCCGTGGGA GCTGGACGTC ATGCTCAAGT GGCTCCCATG ATAATATACA GACGATCCAG 
CACCAGAGAA GCTGGGAGAC TCTTCCATTC GGGCATACTC ACTTTGATTA TTCAGGGGAT 
CCTGCAGGTT TATGGGCATC AAGCAGCCAT ATGGACCAAA TTATGTTTTC TGATCATAGC 
ACAAAGTATA ACAGGCAAAA TCAAAGTAGA GAGAGCCTTG AACAAGCCCA GTCCCGAGCA 
AGCTGGGCGT CTTCCACAGG TTACTGGGGA GAAGACTCAG AAGGTGACAC AGGCACAATA 
AAGCGGAGGG GTGGAAAGGA TGTTTCCATT GAAGCCGAAA GCAGTAGCCT AACGTCTGTG 
ACTACGGAAG AAACCAAGCC TGTCCCCATG CCTGCCCACA TAGCTGTGGC ATCAAGTACT 
ACAAAGGGGC TCATTGCACG AAAGGAGGGC AGGTATCGAG AGCCCCCGCC CACCCCTCCC 
GGCTACATTG GAATTCCCAT TACTGACTTT CCAGAAGGGC ACTCCCATCC AGCCAGGAAA 
CCGCCGGACT ACAACGTGGC CCTTCAGAGA TCGCGGATGG TCGCACGATC CTCCGACACA 
GCTGGGCCTT CATCCGTACA GCAGCCACAT GGGCATCCCA CCAGCAGCAG GCCTGTGAAC 
AAACCTCAGT GGCATAAACC GAACGAGTCT GACCCGCGCC TCGCCCCCTA TCAGTCCCAA 
GGGTTTTCCA CCGAGGAGGA TGAAGATGAA CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005263359.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263416.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu39236
Clone ID Related Accession (Same CDS sequence)	XM_005263359.3 , XM_005263359.4
Accession Version	XM_005263359.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263359.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC 
TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG 
ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT 
GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA 
CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG 
GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA 
CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA 
GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT 
GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG 
AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA 
GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG 
TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG 
GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG 
AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG 
GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG 
GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC 
ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG 
GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG 
CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT 
CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT 
TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263416.1
CDS	97..5178
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005263359.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263359.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC 
TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG 
ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT 
GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA 
CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG 
GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA 
CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA 
GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT 
GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG 
AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA 
GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG 
TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG 
GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG 
AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG 
GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG 
GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC 
ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG 
GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG 
CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT 
CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT 
TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005263359.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263416.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OHu39236
Clone ID Related Accession (Same CDS sequence)	XM_005263359.3 , XM_005263359.4
Accession Version	XM_005263359.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263359.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC 
TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG 
ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT 
GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA 
CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG 
GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA 
CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA 
GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT 
GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG 
AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA 
GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG 
TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG 
GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG 
AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG 
GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG 
GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC 
ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG 
GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG 
CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT 
CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT 
TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263416.1
CDS	95..5176
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005263359.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263359.4

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGTCTCT CAGCCAGGGT AGTACAAATG CAACAGTGCT AGATGTTGCT 
CAGACAGGTG GTCATAAAAA GCGGGTACGT CGTAGTTCCT TTCTCAATGC CAAAAAGCTT 
TATGAAGATG CCCAAATGGC TCGAAAAGTG AAGCAGTACC TTTCCAATTT GGAGCTAGAA 
ATGGACGAGG AGAGTCTTCA GACATTATCT CTGCAGTGTG AGCCAGCAAC CAACACATTG 
CCTAAGAATC CTGGTGACAA AAAGCCTGTC AAATCCGAGA CCTCTCCAGT AGCTCCAAGG 
GCAGGGTCAC AACAGAAAGC TCAGTCCCTG CCACAGCCCC AGCAGCAGCC ACCACCAGCA 
CATAAAATCA ACCAGGGACT ACAGGTTCCC GCCGTGTCCC TTTATCCTTC ACGGAAGAAA 
GTGCCCGTAA AGGATCTCCC ACCTTTTGGC ATAAACTCTC CACAAGCTTT AAAAAAAATT 
CTTTCTTTGT CTGAAGAAGG AAGTTTGGAA CGTCACAAGA AACAGGCTGA AGATACAATA 
TCAAATGCAT CTTCGCAGCT TTCTTCTCCT CCTACTTCTC CACAGAGTTC TCCAAGGAAA 
GGTGGCAGTG GCAATCAGCT GAGATCTTTT GGCTCCGGGC AGTTGGACTT AACCAGTTCC 
TCCTCTTCTC TTGGAAGTGA GAACAGTAAC AAGAATAACA ATGCACCACG GACCTATGGG 
ATAGGCTATA CTTTGGCTCC CAGTGGTACT GTGGATAATT TTTCAGATTC TGGTCACAGT 
GAAATTTCTT CACGATCCAG TATTGTTAGC AATTCGTCTT TTGACTCAGT GCCAGTCTCA 
CTGCACGATG AGAGGCGCCA GAGGCATTCT GTCAGCATCG TGGAAACAAA CCTAGGGATG 
GGCAGGATGG AGAGGCGGAC CATGATTGAA CCTGATCAGT ATAGCTTGGG GTCCTATGCA 
CCAATGTCCG AGGGCCGAGG CTTATATGCT ACAGCTACAG TAATTTCTTC TCCAAGCACA 
GAGGAACTTT CCCAGGATCA GGGGGATCGC GCGTCACTTG ATGCTGCTGA CAGTGGCCGT 
GGGAGCTGGA CGTCATGCTC AAGTGGCTCC CATGATAATA TACAGACGAT CCAGCACCAG 
AGAAGCTGGG AGACTCTTCC ATTCGGGCAT ACTCACTTTG ATTATTCAGG GGATCCTGCA 
GGTTTATGGG CATCAAGCAG CCATATGGAC CAAATTATGT TTTCTGATCA TAGCACAAAG 
TATAACAGGC AAAATCAAAG TAGAGAGAGC CTTGAACAAG CCCAGTCCCG AGCAAGCTGG 
GCGTCTTCCA CAGGTTACTG GGGAGAAGAC TCAGAAGGTG ACACAGGCAC AATAAAGCGG 
AGGGGTGGAA AGGATGTTTC CATTGAAGCC GAAAGCAGTA GCCTAACGTC TGTGACTACG 
GAAGAAACCA AGCCTGTCCC CATGCCTGCC CACATAGCTG TGGCATCAAG TACTACAAAG 
GGGCTCATTG CACGAAAGGA GGGCAGGTAT CGAGAGCCCC CGCCCACCCC TCCCGGCTAC 
ATTGGAATTC CCATTACTGA CTTTCCAGAA GGGCACTCCC ATCCAGCCAG GAAACCGCCG 
GACTACAACG TGGCCCTTCA GAGATCGCGG ATGGTCGCAC GATCCTCCGA CACAGCTGGG 
CCTTCATCCG TACAGCAGCC ACATGGGCAT CCCACCAGCA GCAGGCCTGT GAACAAACCT 
CAGTGGCATA AACCGAACGA GTCTGACCCG CGCCTCGCCC CCTATCAGTC CCAAGGGTTT 
TCCACCGAGG AGGATGAAGA TGAACAAGTT TCTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_006714421.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714484.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OHu39237
Clone ID Related Accession (Same CDS sequence)	XM_006714421.3 , NM_001351724.1 , NM_001351724.2
Accession Version	XM_006714421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714484.1
CDS	62..5119
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_006714421.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714421.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGTGGC AGTGGCAATC AGCTGAGATC TTTTGGCTCC 
GGGCAGTTGG ACTTAACCAG TTCCTCCTCT TCTCTTGGAA GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005263360.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263417.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

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CloneID	OHu39238
Clone ID Related Accession (Same CDS sequence)	XM_005263360.3
Accession Version	XM_005263360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263417.1
CDS	89..5071
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005263360.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263360.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGGAATG CACTAAATCT 
CAGCTTCCTG CAGATTTCAC AAAACTGCAT CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCTT CTAGCCATTC AGGATGTAGT ATCACTAGTG ATTCTGGGAG CAGCAGTCTT 
TCTGATATCT ACCAGGCCAC AGAAAGCGAG GCTGGTGATA TGGACCTGAG TGGGTTGCCA 
GAAACAGCAG TGGATTCCGA AGACGACGAC GATGAAGAAG ACATTGAGAG AGCATCAGAT 
CCTCTGATGA GCAGGGACAT TGTGAGAGAC TGCCTAGAGA AGGACCCAAT TGACCGGACA 
GATGATGACA TTGAACAACT CTTGGAATTT ATGCACCAGT TGCCTGCTTT TGCCAATATG 
ACAATGTCAG TGAGGCGAGA ACTCTGTGCT GTGATGGTGT TCGCAGTGGT GGAAAGAGCA 
GGGACCATAG TGTTAAATGA TGGTGAAGAG CTGGACTCCT GGTCAGTGAT TCTCAATGGA 
TCTGTGGAAG TGACTTATCC AGATGGAAAA GCAGAAATAC TGTGCATGGG AAATAGTTTT 
GGTGTCTCTC CTACCATGGA CAAAGAATAC ATGAAAGGAG TGATGAGAAC AAAGGTGGAT 
GACTGCCAGT TTGTCTGCAT AGCCCAGCAA GATTACTGCC GTATTCTCAA TCAAGTAGAA 
AAGAACATGC AAAAAGTTGA AGAGGAAGGA GAGATTGTTA TGGTGAAAGA ACACCGAGAA 
CTTGATCGAA CTGGAACAAG AAAGGGACAC ATTGTCATCA AGGGTACCTC AGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCATTCAGTA GTAGATCCAA CATTCATAGA AGACTTTCTG 
TTGACCTATA GGACTTTTCT TTCTAGCCCA ATGGAAGTGG GCAAAAAGTT ATTGGAGTGG 
TTTAATGACC CGAGCCTCAG GGATAAGGTT ACACGGGTAG TATTATTGTG GGTAAATAAT 
CACTTCAATG ACTTTGAAGG AGATCCTGCA ATGACTCGAT TTTTAGAAGA ATTTGAAAAC 
AATCTGGAAA GAGAGAAAAT GGGTGGACAC CTAAGGCTGT TGAATATCGC GTGTGCTGCT 
AAAGCAAAAA GAAGATTGAT GACGTTAACA AAACCATCCC GAGAAGCTCC TTTGCCTTTT 
ATCTTACTTG GAGGCTCTGA GAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTCAGGT 
AGCAAAGCAA CTGAAGCAGG CTTGAAACGG GGGGATCAGA TATTAGAAGT AAATGGCCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCT ATGGAAATTC TTAGAAATAA CACACATTTA 
TCTATCACTG TGAAAACCAA TTTATTTGTA TTTAAAGAAC TTCTAACAAG ATTGTCAGAA 
GAGAAAAGAA ATGGTGCCCC CCACCTTCCT AAAATTGGTG ACATTAAAAA GGCCAGTCGC 
TACTCCATTC CAGATCTTGC TGTAGATGTA GAACAGGTGA TAGGACTTGA AAAAGTGAAC 
AAAAAAAGTA AAGCCAACAC TGTGGGAGGA AGGAACAAGC TGAAAAAGAT ACTCGACAAG 
ACTCGGATCA GTATCTTGCC ACAGAAACCA TACAATGATA TTGGGATTGG TCAGTCTCAA 
GATGACAGCA TAGTAGGATT AAGGCAGACA AAGCACATCC CAACTGCATT GCCTGTCAGT 
GGAACCTTAT CATCCAGTAA TCCTGATTTA TTGCAGTCAC ATCATCGCAT TTTAGACTTC 
AGTGCTACTC CTGACTTGCC AGATCAAGTG CTAAGGGTTT TTAAGGCTGA TCAGCAAAGC 
CGCTACATCA TGATCAGTAA GGACACTACA GCAAAGGAAG TGGTCATTCA GGCTATCAGG 
GAGTTTGCTG TTACTGCCAC CCCGGATCAA TATTCACTAT GTGAGGTCTC TGTCACACCT 
GAGGGAGTAA TCAAACAAAG AAGACTTCCA GATCAGCTTT CCAAACTTGC AGACAGAATA 
CAACTGAGTG GAAGGTATTA TCTGAAAAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AGGAGTTGTT GAGAGAGAGT CAAATTTCCC TCCTTCAGCT CAGCACTGTG 
GAAGTTGCAA CACAGCTCTC TATGCGAAAT TTTGAACTCT TTCGCAACAT TGAACCTACT 
GAATATATAG ATGATTTATT TAAACTCAGA TCAAAAACCA GCTGTGCCAA CCTGAAGAGA 
TTTGAAGAAG TCATTAACCA GGAAACATTT TGGGTAGCAT CTGAAATTCT CAGAGAAACA 
AACCAGCTGA AGAGGATGAA GATCATTAAG CATTTCATCA AGATAGCACT GCACTGTAGG 
GAATGCAAGA ATTTTAACTC AATGTTTGCA ATCATCAGTG GCCTAAACCT GGCACCAGTG 
GCAAGACTGC GAACGACCTG GGAGAAACTT CCCAATAAAT ACGAAAAACT ATTTCAAGAT 
CTCCAAGACC TGTTTGATCC TTCCAGAAAC ATGGCAAAAT ATCGTAATGT TCTCAATAGT 
CAAAATCTAC AACCTCCCAT AATCCCTCTA TTCCCAGTTA TCAAAAAGGA TCTCACCTTC 
CTTCACGAAG GAAATGACTC AAAAGTAGAC GGGCTGGTCA ATTTTGAGAA GCTAAGGATG 
ATTGCAAAAG AAATTCGTCA CGTTGGCCGA ATGGCTTCAG TGAACATGGA CCCTGCCCTC 
ATGTTCAGGA CTCGGAAGAA GAAATGGCGG AGTTTGGGGT CTCTCAGCCA GGGTAGTACA 
AATGCAACAG TGCTAGATGT TGCTCAGACA GGTGGTCATA AAAAGCGGGT ACGTCGTAGT 
TCCTTTCTCA ATGCCAAAAA GCTTTATGAA GATGCCCAAA TGGCTCGAAA AGTGAAGCAG 
TACCTTTCCA ATTTGGAGCT AGAAATGGAC GAGGAGAGTC TTCAGACATT ATCTCTGCAG 
TGTGAGCCAG CAACCAACAC ATTGCCTAAG AATCCTGGTG ACAAAAAGCC TGTCAAATCC 
GAGACCTCTC CAGTAGCTCC AAGGGCAGGG TCACAACAGA AAGCTCAGTC CCTGCCACAG 
CCCCAGCAGC AGCCACCACC AGCACATAAA ATCAACCAGG GACTACAGGT TCCCGCCGTG 
TCCCTTTATC CTTCACGGAA GAAAGTGCCC GTAAAGGATC TCCCACCTTT TGGCATAAAC 
TCTCCACAAG CTTTAAAAAA AATTCTTTCT TTGTCTGAAG AAGGAAGTTT GGAACGTCAC 
AAGAAACAGG CTGAAGATAC AATATCAAAT GCATCTTCGC AGCTTTCTTC TCCTCCTACT 
TCTCCACAGA GTTCTCCAAG GAAAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_005263361.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263418.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

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CloneID	OHu39239
Clone ID Related Accession (Same CDS sequence)	XM_005263361.3
Accession Version	XM_005263361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC 
ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT 
TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC 
GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC 
ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA 
CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA 
GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT 
GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG 
GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA 
AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA 
GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT 
CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG 
ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT 
GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA 
GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG 
CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC 
CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT 
GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC 
ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT 
AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG 
CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT 
AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC 
ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA 
AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT 
TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG 
TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC 
TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA 
TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC 
CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC 
TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC 
ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC 
TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT 
CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT 
GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA 
AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG 
CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC 
ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT 
CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA 
CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG 
AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA 
AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT 
ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA 
AGGAAAGGCT ATACTTTGGC TCCCAGTGGT ACTGTGGATA ATTTTTCAGA TTCTGGTCAC 
AGTGAAATTT CTTCACGATC CAGTATTGTT AGCAATTCGT CTTTTGACTC AGTGCCAGTC 
TCACTGCACG ATGAGAGGCG CCAGAGGCAT TCTGTCAGCA TCGTGGAAAC AAACCTAGGG 
ATGGGCAGGA TGGAGAGGCG GACCATGATT GAACCTGATC AGTATAGCTT GGGGTCCTAT 
GCACCAATGT CCGAGGGCCG AGGCTTATAT GCTACAGCTA CAGTAATTTC TTCTCCAAGC 
ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT CGCGCGTCAC TTGATGCTGC TGACAGTGGC 
CGTGGGAGCT GGACGTCATG CTCAAGTGGC TCCCATGATA ATATACAGAC GATCCAGCAC 
CAGAGAAGCT GGGAGACTCT TCCATTCGGG CATACTCACT TTGATTATTC AGGGGATCCT 
GCAGGTTTAT GGGCATCAAG CAGCCATATG GACCAAATTA TGTTTTCTGA TCATAGCACA 
AAGTATAACA GGCAAAATCA AAGTAGAGAG AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC 
TGGGCGTCTT CCACAGGTTA CTGGGGAGAA GACTCAGAAG GTGACACAGG CACAATAAAG 
CGGAGGGGTG GAAAGGATGT TTCCATTGAA GCCGAAAGCA GTAGCCTAAC GTCTGTGACT 
ACGGAAGAAA CCAAGCCTGT CCCCATGCCT GCCCACATAG CTGTGGCATC AAGTACTACA 
AAGGGGCTCA TTGCACGAAA GGAGGGCAGG TATCGAGAGC CCCCGCCCAC CCCTCCCGGC 
TACATTGGAA TTCCCATTAC TGACTTTCCA GAAGGGCACT CCCATCCAGC CAGGAAACCG 
CCGGACTACA ACGTGGCCCT TCAGAGATCG CGGATGGTCG CACGATCCTC CGACACAGCT 
GGGCCTTCAT CCGTACAGCA GCCACATGGG CATCCCACCA GCAGCAGGCC TGTGAACAAA 
CCTCAGTGGC ATAAACCGAA CGAGTCTGAC CCGCGCCTCG CCCCCTATCA GTCCCAAGGG 
TTTTCCACCG AGGAGGATGA AGATGAACAA GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263418.1
CDS	95..5059
Translation	MASYVDNSFRQAVMKNPPERTPQDLEIVYSYLHGMEALSNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_005263361.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263361.3

ORF Insert Sequence:

ATGGCGTCCT ACGTAGATAA CAGCTTCCGC CAGGCGGTGA TGAAGAATCC CCCCGAAAGG 
ACCCCCCAGG ATCTGGAAAT AGTATATTCC TATTTACATG GTATGGAAGC CTTATCAAAC 
TTGAGGGAGC ATCAACTTAG GTTAATGTGT GAAACTGTGA GATATGAGAG ACACGAAGCA 
AATGAAGTTT TATACTACCC TGATGATATT GGGACCTGCT GGTATATCCT TCTTTCTGGT 
TCCGTGTTCA TCAAGGAATC CATGTTTCTT CCAAGAAGCA GTTTTGGCAA GCGTTCTGCA 
GGAAGTTTTA GGCGTGGCTG TGAATGCATT GTTTTAGAGC CTTCTGAAAT GATTGTGGTG 
GACTATATGG ATGAAAATGA AGAATATTTT CAGCGGCAAG CTTCCCATAG ACAGTCTCGA 
AGGAGATTTA GAAAAATCAA CCAGAAAGGT GAAAGACAAA CAATTATTGA CACTGTGGAT 
CCTTATCCCA TGGGCAAACC TCCTTTGCCT AGAGGCTATC ACACGCTTCC TGCAGATTTC 
ACAAAACTGC ATCTTACTGA CAGTCTCCAC CCACAGGTGA CCCACGTTTC TTCTAGCCAT 
TCAGGATGTA GTATCACTAG TGATTCTGGG AGCAGCAGTC TTTCTGATAT CTACCAGGCC 
ACAGAAAGCG AGGCTGGTGA TATGGACCTG AGTGGGTTGC CAGAAACAGC AGTGGATTCC 
GAAGACGACG ACGATGAAGA AGACATTGAG AGAGCATCAG ATCCTCTGAT GAGCAGGGAC 
ATTGTGAGAG ACTGCCTAGA GAAGGACCCA ATTGACCGGA CAGATGATGA CATTGAACAA 
CTCTTGGAAT TTATGCACCA GTTGCCTGCT TTTGCCAATA TGACAATGTC AGTGAGGCGA 
GAACTCTGTG CTGTGATGGT GTTCGCAGTG GTGGAAAGAG CAGGGACCAT AGTGTTAAAT 
GATGGTGAAG AGCTGGACTC CTGGTCAGTG ATTCTCAATG GATCTGTGGA AGTGACTTAT 
CCAGATGGAA AAGCAGAAAT ACTGTGCATG GGAAATAGTT TTGGTGTCTC TCCTACCATG 
GACAAAGAAT ACATGAAAGG AGTGATGAGA ACAAAGGTGG ATGACTGCCA GTTTGTCTGC 
ATAGCCCAGC AAGATTACTG CCGTATTCTC AATCAAGTAG AAAAGAACAT GCAAAAAGTT 
GAAGAGGAAG GAGAGATTGT TATGGTGAAA GAACACCGAG AACTTGATCG AACTGGAACA 
AGAAAGGGAC ACATTGTCAT CAAGGGTACC TCAGAAAGGT TAACAATGCA TTTGGTGGAA 
GAGCATTCAG TAGTAGATCC AACATTCATA GAAGACTTTC TGTTGACCTA TAGGACTTTT 
CTTTCTAGCC CAATGGAAGT GGGCAAAAAG TTATTGGAGT GGTTTAATGA CCCGAGCCTC 
AGGGATAAGG TTACACGGGT AGTATTATTG TGGGTAAATA ATCACTTCAA TGACTTTGAA 
GGAGATCCTG CAATGACTCG ATTTTTAGAA GAATTTGAAA ACAATCTGGA AAGAGAGAAA 
ATGGGTGGAC ACCTAAGGCT GTTGAATATC GCGTGTGCTG CTAAAGCAAA AAGAAGATTG 
ATGACGTTAA CAAAACCATC CCGAGAAGCT CCTTTGCCTT TTATCTTACT TGGAGGCTCT 
GAGAAGGGAT TTGGAATCTT TGTTGACAGT GTAGATTCAG GTAGCAAAGC AACTGAAGCA 
GGCTTGAAAC GGGGGGATCA GATATTAGAA GTAAATGGCC AAAACTTTGA AAACATTCAG 
CTGTCAAAAG CTATGGAAAT TCTTAGAAAT AACACACATT TATCTATCAC TGTGAAAACC 
AATTTATTTG TATTTAAAGA ACTTCTAACA AGATTGTCAG AAGAGAAAAG AAATGGTGCC 
CCCCACCTTC CTAAAATTGG TGACATTAAA AAGGCCAGTC GCTACTCCAT TCCAGATCTT 
GCTGTAGATG TAGAACAGGT GATAGGACTT GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC 
ACTGTGGGAG GAAGGAACAA GCTGAAAAAG ATACTCGACA AGACTCGGAT CAGTATCTTG 
CCACAGAAAC CATACAATGA TATTGGGATT GGTCAGTCTC AAGATGACAG CATAGTAGGA 
TTAAGGCAGA CAAAGCACAT CCCAACTGCA TTGCCTGTCA GTGGAACCTT ATCATCCAGT 
AATCCTGATT TATTGCAGTC ACATCATCGC ATTTTAGACT TCAGTGCTAC TCCTGACTTG 
CCAGATCAAG TGCTAAGGGT TTTTAAGGCT GATCAGCAAA GCCGCTACAT CATGATCAGT 
AAGGACACTA CAGCAAAGGA AGTGGTCATT CAGGCTATCA GGGAGTTTGC TGTTACTGCC 
ACCCCGGATC AATATTCACT ATGTGAGGTC TCTGTCACAC CTGAGGGAGT AATCAAACAA 
AGAAGACTTC CAGATCAGCT TTCCAAACTT GCAGACAGAA TACAACTGAG TGGAAGGTAT 
TATCTGAAAA ACAACATGGA AACAGAAACT CTTTGTTCAG ATGAAGATGC TCAGGAGTTG 
TTGAGAGAGA GTCAAATTTC CCTCCTTCAG CTCAGCACTG TGGAAGTTGC AACACAGCTC 
TCTATGCGAA ATTTTGAACT CTTTCGCAAC ATTGAACCTA CTGAATATAT AGATGATTTA 
TTTAAACTCA GATCAAAAAC CAGCTGTGCC AACCTGAAGA GATTTGAAGA AGTCATTAAC 
CAGGAAACAT TTTGGGTAGC ATCTGAAATT CTCAGAGAAA CAAACCAGCT GAAGAGGATG 
AAGATCATTA AGCATTTCAT CAAGATAGCA CTGCACTGTA GGGAATGCAA GAATTTTAAC 
TCAATGTTTG CAATCATCAG TGGCCTAAAC CTGGCACCAG TGGCAAGACT GCGAACGACC 
TGGGAGAAAC TTCCCAATAA ATACGAAAAA CTATTTCAAG ATCTCCAAGA CCTGTTTGAT 
CCTTCCAGAA ACATGGCAAA ATATCGTAAT GTTCTCAATA GTCAAAATCT ACAACCTCCC 
ATAATCCCTC TATTCCCAGT TATCAAAAAG GATCTCACCT TCCTTCACGA AGGAAATGAC 
TCAAAAGTAG ACGGGCTGGT CAATTTTGAG AAGCTAAGGA TGATTGCAAA AGAAATTCGT 
CACGTTGGCC GAATGGCTTC AGTGAACATG GACCCTGCCC TCATGTTCAG GACTCGGAAG 
AAGAAATGGC GGAGTTTGGG GTCTCTCAGC CAGGGTAGTA CAAATGCAAC AGTGCTAGAT 
GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG GTACGTCGTA GTTCCTTTCT CAATGCCAAA 
AAGCTTTATG AAGATGCCCA AATGGCTCGA AAAGTGAAGC AGTACCTTTC CAATTTGGAG 
CTAGAAATGG ACGAGGAGAG TCTTCAGACA TTATCTCTGC AGTGTGAGCC AGCAACCAAC 
ACATTGCCTA AGAATCCTGG TGACAAAAAG CCTGTCAAAT CCGAGACCTC TCCAGTAGCT 
CCAAGGGCAG GGTCACAACA GAAAGCTCAG TCCCTGCCAC AGCCCCAGCA GCAGCCACCA 
CCAGCACATA AAATCAACCA GGGACTACAG GTTCCCGCCG TGTCCCTTTA TCCTTCACGG 
AAGAAAGTGC CCGTAAAGGA TCTCCCACCT TTTGGCATAA ACTCTCCACA AGCTTTAAAA 
AAAATTCTTT CTTTGTCTGA AGAAGGAAGT TTGGAACGTC ACAAGAAACA GGCTGAAGAT 
ACAATATCAA ATGCATCTTC GCAGCTTTCT TCTCCTCCTA CTTCTCCACA GAGTTCTCCA 
AGGAAAGGCT ATACTTTGGC TCCCAGTGGT ACTGTGGATA ATTTTTCAGA TTCTGGTCAC 
AGTGAAATTT CTTCACGATC CAGTATTGTT AGCAATTCGT CTTTTGACTC AGTGCCAGTC 
TCACTGCACG ATGAGAGGCG CCAGAGGCAT TCTGTCAGCA TCGTGGAAAC AAACCTAGGG 
ATGGGCAGGA TGGAGAGGCG GACCATGATT GAACCTGATC AGTATAGCTT GGGGTCCTAT 
GCACCAATGT CCGAGGGCCG AGGCTTATAT GCTACAGCTA CAGTAATTTC TTCTCCAAGC 
ACAGAGGAAC TTTCCCAGGA TCAGGGGGAT CGCGCGTCAC TTGATGCTGC TGACAGTGGC 
CGTGGGAGCT GGACGTCATG CTCAAGTGGC TCCCATGATA ATATACAGAC GATCCAGCAC 
CAGAGAAGCT GGGAGACTCT TCCATTCGGG CATACTCACT TTGATTATTC AGGGGATCCT 
GCAGGTTTAT GGGCATCAAG CAGCCATATG GACCAAATTA TGTTTTCTGA TCATAGCACA 
AAGTATAACA GGCAAAATCA AAGTAGAGAG AGCCTTGAAC AAGCCCAGTC CCGAGCAAGC 
TGGGCGTCTT CCACAGGTTA CTGGGGAGAA GACTCAGAAG GTGACACAGG CACAATAAAG 
CGGAGGGGTG GAAAGGATGT TTCCATTGAA GCCGAAAGCA GTAGCCTAAC GTCTGTGACT 
ACGGAAGAAA CCAAGCCTGT CCCCATGCCT GCCCACATAG CTGTGGCATC AAGTACTACA 
AAGGGGCTCA TTGCACGAAA GGAGGGCAGG TATCGAGAGC CCCCGCCCAC CCCTCCCGGC 
TACATTGGAA TTCCCATTAC TGACTTTCCA GAAGGGCACT CCCATCCAGC CAGGAAACCG 
CCGGACTACA ACGTGGCCCT TCAGAGATCG CGGATGGTCG CACGATCCTC CGACACAGCT 
GGGCCTTCAT CCGTACAGCA GCCACATGGG CATCCCACCA GCAGCAGGCC TGTGAACAAA 
CCTCAGTGGC ATAAACCGAA CGAGTCTGAC CCGCGCCTCG CCCCCTATCA GTCCCAAGGG 
TTTTCCACCG AGGAGGATGA AGATGAACAA GTTTCTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_006714422.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714485.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

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CloneID	OHu39240
Clone ID Related Accession (Same CDS sequence)	XM_006714422.3 , XM_006714422.4 , XM_011532426.2
Accession Version	XM_006714422.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714485.1
CDS	658..5415
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_006714422.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714422.3

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_006714422.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714485.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

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CloneID	OHu39240
Clone ID Related Accession (Same CDS sequence)	XM_006714422.3 , XM_006714422.4 , XM_011532426.2
Accession Version	XM_006714422.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714422.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714485.1
CDS	2169..6926
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_006714422.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714422.4

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011532426.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530728.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu39240
Clone ID Related Accession (Same CDS sequence)	XM_006714422.3 , XM_006714422.4 , XM_011532426.2
Accession Version	XM_011532426.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530728.1
CDS	1210..5967
Translation	MIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011532426.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532426.2

ORF Insert Sequence:

ATGATTGTGG TGGACTATAT GGATGAAAAT GAAGAATATT TTCAGCGGCA AGCTTCCCAT 
AGACAGTCTC GAAGGAGATT TAGAAAAATC AACCAGAAAG GTGAAAGACA AACAATTATT 
GACACTGTGG ATCCTTATCC CATGGGCAAA CCTCCTTTGC CTAGAGGCTA TCACACGGAA 
TGCACTAAAT CTCAGCTTCC TGCAGATTTC ACAAAACTGC ATCTTACTGA CAGTCTCCAC 
CCACAGGTGA CCCACGTTTC TTCTAGCCAT TCAGGATGTA GTATCACTAG TGATTCTGGG 
AGCAGCAGTC TTTCTGATAT CTACCAGGCC ACAGAAAGCG AGGCTGGTGA TATGGACCTG 
AGTGGGTTGC CAGAAACAGC AGTGGATTCC GAAGACGACG ACGATGAAGA AGACATTGAG 
AGAGCATCAG ATCCTCTGAT GAGCAGGGAC ATTGTGAGAG ACTGCCTAGA GAAGGACCCA 
ATTGACCGGA CAGATGATGA CATTGAACAA CTCTTGGAAT TTATGCACCA GTTGCCTGCT 
TTTGCCAATA TGACAATGTC AGTGAGGCGA GAACTCTGTG CTGTGATGGT GTTCGCAGTG 
GTGGAAAGAG CAGGGACCAT AGTGTTAAAT GATGGTGAAG AGCTGGACTC CTGGTCAGTG 
ATTCTCAATG GATCTGTGGA AGTGACTTAT CCAGATGGAA AAGCAGAAAT ACTGTGCATG 
GGAAATAGTT TTGGTGTCTC TCCTACCATG GACAAAGAAT ACATGAAAGG AGTGATGAGA 
ACAAAGGTGG ATGACTGCCA GTTTGTCTGC ATAGCCCAGC AAGATTACTG CCGTATTCTC 
AATCAAGTAG AAAAGAACAT GCAAAAAGTT GAAGAGGAAG GAGAGATTGT TATGGTGAAA 
GAACACCGAG AACTTGATCG AACTGGAACA AGAAAGGGAC ACATTGTCAT CAAGGGTACC 
TCAGAAAGGT TAACAATGCA TTTGGTGGAA GAGCATTCAG TAGTAGATCC AACATTCATA 
GAAGACTTTC TGTTGACCTA TAGGACTTTT CTTTCTAGCC CAATGGAAGT GGGCAAAAAG 
TTATTGGAGT GGTTTAATGA CCCGAGCCTC AGGGATAAGG TTACACGGGT AGTATTATTG 
TGGGTAAATA ATCACTTCAA TGACTTTGAA GGAGATCCTG CAATGACTCG ATTTTTAGAA 
GAATTTGAAA ACAATCTGGA AAGAGAGAAA ATGGGTGGAC ACCTAAGGCT GTTGAATATC 
GCGTGTGCTG CTAAAGCAAA AAGAAGATTG ATGACGTTAA CAAAACCATC CCGAGAAGCT 
CCTTTGCCTT TTATCTTACT TGGAGGCTCT GAGAAGGGAT TTGGAATCTT TGTTGACAGT 
GTAGATTCAG GTAGCAAAGC AACTGAAGCA GGCTTGAAAC GGGGGGATCA GATATTAGAA 
GTAAATGGCC AAAACTTTGA AAACATTCAG CTGTCAAAAG CTATGGAAAT TCTTAGAAAT 
AACACACATT TATCTATCAC TGTGAAAACC AATTTATTTG TATTTAAAGA ACTTCTAACA 
AGATTGTCAG AAGAGAAAAG AAATGGTGCC CCCCACCTTC CTAAAATTGG TGACATTAAA 
AAGGCCAGTC GCTACTCCAT TCCAGATCTT GCTGTAGATG TAGAACAGGT GATAGGACTT 
GAAAAAGTGA ACAAAAAAAG TAAAGCCAAC ACTGTGGGAG GAAGGAACAA GCTGAAAAAG 
ATACTCGACA AGACTCGGAT CAGTATCTTG CCACAGAAAC CATACAATGA TATTGGGATT 
GGTCAGTCTC AAGATGACAG CATAGTAGGA TTAAGGCAGA CAAAGCACAT CCCAACTGCA 
TTGCCTGTCA GTGGAACCTT ATCATCCAGT AATCCTGATT TATTGCAGTC ACATCATCGC 
ATTTTAGACT TCAGTGCTAC TCCTGACTTG CCAGATCAAG TGCTAAGGGT TTTTAAGGCT 
GATCAGCAAA GCCGCTACAT CATGATCAGT AAGGACACTA CAGCAAAGGA AGTGGTCATT 
CAGGCTATCA GGGAGTTTGC TGTTACTGCC ACCCCGGATC AATATTCACT ATGTGAGGTC 
TCTGTCACAC CTGAGGGAGT AATCAAACAA AGAAGACTTC CAGATCAGCT TTCCAAACTT 
GCAGACAGAA TACAACTGAG TGGAAGGTAT TATCTGAAAA ACAACATGGA AACAGAAACT 
CTTTGTTCAG ATGAAGATGC TCAGGAGTTG TTGAGAGAGA GTCAAATTTC CCTCCTTCAG 
CTCAGCACTG TGGAAGTTGC AACACAGCTC TCTATGCGAA ATTTTGAACT CTTTCGCAAC 
ATTGAACCTA CTGAATATAT AGATGATTTA TTTAAACTCA GATCAAAAAC CAGCTGTGCC 
AACCTGAAGA GATTTGAAGA AGTCATTAAC CAGGAAACAT TTTGGGTAGC ATCTGAAATT 
CTCAGAGAAA CAAACCAGCT GAAGAGGATG AAGATCATTA AGCATTTCAT CAAGATAGCA 
CTGCACTGTA GGGAATGCAA GAATTTTAAC TCAATGTTTG CAATCATCAG TGGCCTAAAC 
CTGGCACCAG TGGCAAGACT GCGAACGACC TGGGAGAAAC TTCCCAATAA ATACGAAAAA 
CTATTTCAAG ATCTCCAAGA CCTGTTTGAT CCTTCCAGAA ACATGGCAAA ATATCGTAAT 
GTTCTCAATA GTCAAAATCT ACAACCTCCC ATAATCCCTC TATTCCCAGT TATCAAAAAG 
GATCTCACCT TCCTTCACGA AGGAAATGAC TCAAAAGTAG ACGGGCTGGT CAATTTTGAG 
AAGCTAAGGA TGATTGCAAA AGAAATTCGT CACGTTGGCC GAATGGCTTC AGTGAACATG 
GACCCTGCCC TCATGTTCAG GACTCGGAAG AAGAAATGGC GGAGTTTGGG GTCTCTCAGC 
CAGGGTAGTA CAAATGCAAC AGTGCTAGAT GTTGCTCAGA CAGGTGGTCA TAAAAAGCGG 
GTACGTCGTA GTTCCTTTCT CAATGCCAAA AAGCTTTATG AAGATGCCCA AATGGCTCGA 
AAAGTGAAGC AGTACCTTTC CAATTTGGAG CTAGAAATGG ACGAGGAGAG TCTTCAGACA 
TTATCTCTGC AGTGTGAGCC AGCAACCAAC ACATTGCCTA AGAATCCTGG TGACAAAAAG 
CCTGTCAAAT CCGAGACCTC TCCAGTAGCT CCAAGGGCAG GGTCACAACA GAAAGCTCAG 
TCCCTGCCAC AGCCCCAGCA GCAGCCACCA CCAGCACATA AAATCAACCA GGGACTACAG 
GTTCCCGCCG TGTCCCTTTA TCCTTCACGG AAGAAAGTGC CCGTAAAGGA TCTCCCACCT 
TTTGGCATAA ACTCTCCACA AGCTTTAAAA AAAATTCTTT CTTTGTCTGA AGAAGGAAGT 
TTGGAACGTC ACAAGAAACA GGCTGAAGAT ACAATATCAA ATGCATCTTC GCAGCTTTCT 
TCTCCTCCTA CTTCTCCACA GAGTTCTCCA AGGAAAGGTG GCAGTGGCAA TCAGCTGAGA 
TCTTTTGGCT CCGGGCAGTT GGACTTAACC AGTTCCTCCT CTTCTCTTGG AAGTGAGAAC 
AGTAACAAGA ATAACAATGC ACCACGGACC TATGGGATAG GCTATACTTT GGCTCCCAGT 
GGTACTGTGG ATAATTTTTC AGATTCTGGT CACAGTGAAA TTTCTTCACG ATCCAGTATT 
GTTAGCAATT CGTCTTTTGA CTCAGTGCCA GTCTCACTGC ACGATGAGAG GCGCCAGAGG 
CATTCTGTCA GCATCGTGGA AACAAACCTA GGGATGGGCA GGATGGAGAG GCGGACCATG 
ATTGAACCTG ATCAGTATAG CTTGGGGTCC TATGCACCAA TGTCCGAGGG CCGAGGCTTA 
TATGCTACAG CTACAGTAAT TTCTTCTCCA AGCACAGAGG AACTTTCCCA GGATCAGGGG 
GATCGCGCGT CACTTGATGC TGCTGACAGT GGCCGTGGGA GCTGGACGTC ATGCTCAAGT 
GGCTCCCATG ATAATATACA GACGATCCAG CACCAGAGAA GCTGGGAGAC TCTTCCATTC 
GGGCATACTC ACTTTGATTA TTCAGGGGAT CCTGCAGGTT TATGGGCATC AAGCAGCCAT 
ATGGACCAAA TTATGTTTTC TGATCATAGC ACAAAGTATA ACAGGCAAAA TCAAAGTAGA 
GAGAGCCTTG AACAAGCCCA GTCCCGAGCA AGCTGGGCGT CTTCCACAGG TTACTGGGGA 
GAAGACTCAG AAGGTGACAC AGGCACAATA AAGCGGAGGG GTGGAAAGGA TGTTTCCATT 
GAAGCCGAAA GCAGTAGCCT AACGTCTGTG ACTACGGAAG AAACCAAGCC TGTCCCCATG 
CCTGCCCACA TAGCTGTGGC ATCAAGTACT ACAAAGGGGC TCATTGCACG AAAGGAGGGC 
AGGTATCGAG AGCCCCCGCC CACCCCTCCC GGCTACATTG GAATTCCCAT TACTGACTTT 
CCAGAAGGGC ACTCCCATCC AGCCAGGAAA CCGCCGGACT ACAACGTGGC CCTTCAGAGA 
TCGCGGATGG TCGCACGATC CTCCGACACA GCTGGGCCTT CATCCGTACA GCAGCCACAT 
GGGCATCCCA CCAGCAGCAG GCCTGTGAAC AAACCTCAGT GGCATAAACC GAACGAGTCT 
GACCCGCGCC TCGCCCCCTA TCAGTCCCAA GGGTTTTCCA CCGAGGAGGA TGAAGATGAA 
CAAGTTTCTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011532425.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530727.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

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CloneID	OHu63162
Clone ID Related Accession (Same CDS sequence)	XM_011532425.2
Accession Version	XM_011532425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rap guanine nucleotide exchange factor 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAT ATGGGTTATC TGTGGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGG 
CAAGCTTCCC ATAGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGTGAAAGA 
CAAACAATTA TTGACACTGT GGATCCTTAT CCCATGGGCA AACCTCCTTT GCCTAGAGGC 
TATCACACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAACT GCATCTTACT 
GACAGTCTCC ACCCACAGGT GACCCACGTT TCTTCTAGCC ATTCAGGATG TAGTATCACT 
AGTGATTCTG GGAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAAAG CGAGGCTGGT 
GATATGGACC TGAGTGGGTT GCCAGAAACA GCAGTGGATT CCGAAGACGA CGACGATGAA 
GAAGACATTG AGAGAGCATC AGATCCTCTG ATGAGCAGGG ACATTGTGAG AGACTGCCTA 
GAGAAGGACC CAATTGACCG GACAGATGAT GACATTGAAC AACTCTTGGA ATTTATGCAC 
CAGTTGCCTG CTTTTGCCAA TATGACAATG TCAGTGAGGC GAGAACTCTG TGCTGTGATG 
GTGTTCGCAG TGGTGGAAAG AGCAGGGACC ATAGTGTTAA ATGATGGTGA AGAGCTGGAC 
TCCTGGTCAG TGATTCTCAA TGGATCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA 
ATACTGTGCA TGGGAAATAG TTTTGGTGTC TCTCCTACCA TGGACAAAGA ATACATGAAA 
GGAGTGATGA GAACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC 
TGCCGTATTC TCAATCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATT 
GTTATGGTGA AAGAACACCG AGAACTTGAT CGAACTGGAA CAAGAAAGGG ACACATTGTC 
ATCAAGGGTA CCTCAGAAAG GTTAACAATG CATTTGGTGG AAGAGCATTC AGTAGTAGAT 
CCAACATTCA TAGAAGACTT TCTGTTGACC TATAGGACTT TTCTTTCTAG CCCAATGGAA 
GTGGGCAAAA AGTTATTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACACGG 
GTAGTATTAT TGTGGGTAAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT 
CGATTTTTAG AAGAATTTGA AAACAATCTG GAAAGAGAGA AAATGGGTGG ACACCTAAGG 
CTGTTGAATA TCGCGTGTGC TGCTAAAGCA AAAAGAAGAT TGATGACGTT AACAAAACCA 
TCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CTGAGAAGGG ATTTGGAATC 
TTTGTTGACA GTGTAGATTC AGGTAGCAAA GCAACTGAAG CAGGCTTGAA ACGGGGGGAT 
CAGATATTAG AAGTAAATGG CCAAAACTTT GAAAACATTC AGCTGTCAAA AGCTATGGAA 
ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA 
GAACTTCTAA CAAGATTGTC AGAAGAGAAA AGAAATGGTG CCCCCCACCT TCCTAAAATT 
GGTGACATTA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TTGCTGTAGA TGTAGAACAG 
GTGATAGGAC TTGAAAAAGT GAACAAAAAA AGTAAAGCCA ACACTGTGGG AGGAAGGAAC 
AAGCTGAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATACAAT 
GATATTGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACAAAGCAC 
ATCCCAACTG CATTGCCTGT CAGTGGAACC TTATCATCCA GTAATCCTGA TTTATTGCAG 
TCACATCATC GCATTTTAGA CTTCAGTGCT ACTCCTGACT TGCCAGATCA AGTGCTAAGG 
GTTTTTAAGG CTGATCAGCA AAGCCGCTAC ATCATGATCA GTAAGGACAC TACAGCAAAG 
GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT GCTGTTACTG CCACCCCGGA TCAATATTCA 
CTATGTGAGG TCTCTGTCAC ACCTGAGGGA GTAATCAAAC AAAGAAGACT TCCAGATCAG 
CTTTCCAAAC TTGCAGACAG AATACAACTG AGTGGAAGGT ATTATCTGAA AAACAACATG 
GAAACAGAAA CTCTTTGTTC AGATGAAGAT GCTCAGGAGT TGTTGAGAGA GAGTCAAATT 
TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT GCAACACAGC TCTCTATGCG AAATTTTGAA 
CTCTTTCGCA ACATTGAACC TACTGAATAT ATAGATGATT TATTTAAACT CAGATCAAAA 
ACCAGCTGTG CCAACCTGAA GAGATTTGAA GAAGTCATTA ACCAGGAAAC ATTTTGGGTA 
GCATCTGAAA TTCTCAGAGA AACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC 
ATCAAGATAG CACTGCACTG TAGGGAATGC AAGAATTTTA ACTCAATGTT TGCAATCATC 
AGTGGCCTAA ACCTGGCACC AGTGGCAAGA CTGCGAACGA CCTGGGAGAA ACTTCCCAAT 
AAATACGAAA AACTATTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA 
AAATATCGTA ATGTTCTCAA TAGTCAAAAT CTACAACCTC CCATAATCCC TCTATTCCCA 
GTTATCAAAA AGGATCTCAC CTTCCTTCAC GAAGGAAATG ACTCAAAAGT AGACGGGCTG 
GTCAATTTTG AGAAGCTAAG GATGATTGCA AAAGAAATTC GTCACGTTGG CCGAATGGCT 
TCAGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAATG GCGGAGTTTG 
GGGTCTCTCA GCCAGGGTAG TACAAATGCA ACAGTGCTAG ATGTTGCTCA GACAGGTGGT 
CATAAAAAGC GGGTACGTCG TAGTTCCTTT CTCAATGCCA AAAAGCTTTA TGAAGATGCC 
CAAATGGCTC GAAAAGTGAA GCAGTACCTT TCCAATTTGG AGCTAGAAAT GGACGAGGAG 
AGTCTTCAGA CATTATCTCT GCAGTGTGAG CCAGCAACCA ACACATTGCC TAAGAATCCT 
GGTGACAAAA AGCCTGTCAA ATCCGAGACC TCTCCAGTAG CTCCAAGGGC AGGGTCACAA 
CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG CAGCAGCCAC CACCAGCACA TAAAATCAAC 
CAGGGACTAC AGGTTCCCGC CGTGTCCCTT TATCCTTCAC GGAAGAAAGT GCCCGTAAAG 
GATCTCCCAC CTTTTGGCAT AAACTCTCCA CAAGCTTTAA AAAAAATTCT TTCTTTGTCT 
GAAGAAGGAA GTTTGGAACG TCACAAGAAA CAGGCTGAAG ATACAATATC AAATGCATCT 
TCGCAGCTTT CTTCTCCTCC TACTTCTCCA CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC 
AATCAGCTGA GATCTTTTGG CTCCGGGCAG TTGGACTTAA CCAGTTCCTC CTCTTCTCTT 
GGAAGTGAGA ACAGTAACAA GAATAACAAT GCACCACGGA CCTATGGGAT AGGCTATACT 
TTGGCTCCCA GTGGTACTGT GGATAATTTT TCAGATTCTG GTCACAGTGA AATTTCTTCA 
CGATCCAGTA TTGTTAGCAA TTCGTCTTTT GACTCAGTGC CAGTCTCACT GCACGATGAG 
AGGCGCCAGA GGCATTCTGT CAGCATCGTG GAAACAAACC TAGGGATGGG CAGGATGGAG 
AGGCGGACCA TGATTGAACC TGATCAGTAT AGCTTGGGGT CCTATGCACC AATGTCCGAG 
GGCCGAGGCT TATATGCTAC AGCTACAGTA ATTTCTTCTC CAAGCACAGA GGAACTTTCC 
CAGGATCAGG GGGATCGCGC GTCACTTGAT GCTGCTGACA GTGGCCGTGG GAGCTGGACG 
TCATGCTCAA GTGGCTCCCA TGATAATATA CAGACGATCC AGCACCAGAG AAGCTGGGAG 
ACTCTTCCAT TCGGGCATAC TCACTTTGAT TATTCAGGGG ATCCTGCAGG TTTATGGGCA 
TCAAGCAGCC ATATGGACCA AATTATGTTT TCTGATCATA GCACAAAGTA TAACAGGCAA 
AATCAAAGTA GAGAGAGCCT TGAACAAGCC CAGTCCCGAG CAAGCTGGGC GTCTTCCACA 
GGTTACTGGG GAGAAGACTC AGAAGGTGAC ACAGGCACAA TAAAGCGGAG GGGTGGAAAG 
GATGTTTCCA TTGAAGCCGA AAGCAGTAGC CTAACGTCTG TGACTACGGA AGAAACCAAG 
CCTGTCCCCA TGCCTGCCCA CATAGCTGTG GCATCAAGTA CTACAAAGGG GCTCATTGCA 
CGAAAGGAGG GCAGGTATCG AGAGCCCCCG CCCACCCCTC CCGGCTACAT TGGAATTCCC 
ATTACTGACT TTCCAGAAGG GCACTCCCAT CCAGCCAGGA AACCGCCGGA CTACAACGTG 
GCCCTTCAGA GATCGCGGAT GGTCGCACGA TCCTCCGACA CAGCTGGGCC TTCATCCGTA 
CAGCAGCCAC ATGGGCATCC CACCAGCAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA 
CCGAACGAGT CTGACCCGCG CCTCGCCCCC TATCAGTCCC AAGGGTTTTC CACCGAGGAG 
GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530727.1
CDS	261..5030
Translation	MIEYGLSVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPMGKPPLPRGYHTECTKSQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011532425.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532425.2

ORF Insert Sequence:

ATGATTGAAT ATGGGTTATC TGTGGACTAT ATGGATGAAA ATGAAGAATA TTTTCAGCGG 
CAAGCTTCCC ATAGACAGTC TCGAAGGAGA TTTAGAAAAA TCAACCAGAA AGGTGAAAGA 
CAAACAATTA TTGACACTGT GGATCCTTAT CCCATGGGCA AACCTCCTTT GCCTAGAGGC 
TATCACACGG AATGCACTAA ATCTCAGCTT CCTGCAGATT TCACAAAACT GCATCTTACT 
GACAGTCTCC ACCCACAGGT GACCCACGTT TCTTCTAGCC ATTCAGGATG TAGTATCACT 
AGTGATTCTG GGAGCAGCAG TCTTTCTGAT ATCTACCAGG CCACAGAAAG CGAGGCTGGT 
GATATGGACC TGAGTGGGTT GCCAGAAACA GCAGTGGATT CCGAAGACGA CGACGATGAA 
GAAGACATTG AGAGAGCATC AGATCCTCTG ATGAGCAGGG ACATTGTGAG AGACTGCCTA 
GAGAAGGACC CAATTGACCG GACAGATGAT GACATTGAAC AACTCTTGGA ATTTATGCAC 
CAGTTGCCTG CTTTTGCCAA TATGACAATG TCAGTGAGGC GAGAACTCTG TGCTGTGATG 
GTGTTCGCAG TGGTGGAAAG AGCAGGGACC ATAGTGTTAA ATGATGGTGA AGAGCTGGAC 
TCCTGGTCAG TGATTCTCAA TGGATCTGTG GAAGTGACTT ATCCAGATGG AAAAGCAGAA 
ATACTGTGCA TGGGAAATAG TTTTGGTGTC TCTCCTACCA TGGACAAAGA ATACATGAAA 
GGAGTGATGA GAACAAAGGT GGATGACTGC CAGTTTGTCT GCATAGCCCA GCAAGATTAC 
TGCCGTATTC TCAATCAAGT AGAAAAGAAC ATGCAAAAAG TTGAAGAGGA AGGAGAGATT 
GTTATGGTGA AAGAACACCG AGAACTTGAT CGAACTGGAA CAAGAAAGGG ACACATTGTC 
ATCAAGGGTA CCTCAGAAAG GTTAACAATG CATTTGGTGG AAGAGCATTC AGTAGTAGAT 
CCAACATTCA TAGAAGACTT TCTGTTGACC TATAGGACTT TTCTTTCTAG CCCAATGGAA 
GTGGGCAAAA AGTTATTGGA GTGGTTTAAT GACCCGAGCC TCAGGGATAA GGTTACACGG 
GTAGTATTAT TGTGGGTAAA TAATCACTTC AATGACTTTG AAGGAGATCC TGCAATGACT 
CGATTTTTAG AAGAATTTGA AAACAATCTG GAAAGAGAGA AAATGGGTGG ACACCTAAGG 
CTGTTGAATA TCGCGTGTGC TGCTAAAGCA AAAAGAAGAT TGATGACGTT AACAAAACCA 
TCCCGAGAAG CTCCTTTGCC TTTTATCTTA CTTGGAGGCT CTGAGAAGGG ATTTGGAATC 
TTTGTTGACA GTGTAGATTC AGGTAGCAAA GCAACTGAAG CAGGCTTGAA ACGGGGGGAT 
CAGATATTAG AAGTAAATGG CCAAAACTTT GAAAACATTC AGCTGTCAAA AGCTATGGAA 
ATTCTTAGAA ATAACACACA TTTATCTATC ACTGTGAAAA CCAATTTATT TGTATTTAAA 
GAACTTCTAA CAAGATTGTC AGAAGAGAAA AGAAATGGTG CCCCCCACCT TCCTAAAATT 
GGTGACATTA AAAAGGCCAG TCGCTACTCC ATTCCAGATC TTGCTGTAGA TGTAGAACAG 
GTGATAGGAC TTGAAAAAGT GAACAAAAAA AGTAAAGCCA ACACTGTGGG AGGAAGGAAC 
AAGCTGAAAA AGATACTCGA CAAGACTCGG ATCAGTATCT TGCCACAGAA ACCATACAAT 
GATATTGGGA TTGGTCAGTC TCAAGATGAC AGCATAGTAG GATTAAGGCA GACAAAGCAC 
ATCCCAACTG CATTGCCTGT CAGTGGAACC TTATCATCCA GTAATCCTGA TTTATTGCAG 
TCACATCATC GCATTTTAGA CTTCAGTGCT ACTCCTGACT TGCCAGATCA AGTGCTAAGG 
GTTTTTAAGG CTGATCAGCA AAGCCGCTAC ATCATGATCA GTAAGGACAC TACAGCAAAG 
GAAGTGGTCA TTCAGGCTAT CAGGGAGTTT GCTGTTACTG CCACCCCGGA TCAATATTCA 
CTATGTGAGG TCTCTGTCAC ACCTGAGGGA GTAATCAAAC AAAGAAGACT TCCAGATCAG 
CTTTCCAAAC TTGCAGACAG AATACAACTG AGTGGAAGGT ATTATCTGAA AAACAACATG 
GAAACAGAAA CTCTTTGTTC AGATGAAGAT GCTCAGGAGT TGTTGAGAGA GAGTCAAATT 
TCCCTCCTTC AGCTCAGCAC TGTGGAAGTT GCAACACAGC TCTCTATGCG AAATTTTGAA 
CTCTTTCGCA ACATTGAACC TACTGAATAT ATAGATGATT TATTTAAACT CAGATCAAAA 
ACCAGCTGTG CCAACCTGAA GAGATTTGAA GAAGTCATTA ACCAGGAAAC ATTTTGGGTA 
GCATCTGAAA TTCTCAGAGA AACAAACCAG CTGAAGAGGA TGAAGATCAT TAAGCATTTC 
ATCAAGATAG CACTGCACTG TAGGGAATGC AAGAATTTTA ACTCAATGTT TGCAATCATC 
AGTGGCCTAA ACCTGGCACC AGTGGCAAGA CTGCGAACGA CCTGGGAGAA ACTTCCCAAT 
AAATACGAAA AACTATTTCA AGATCTCCAA GACCTGTTTG ATCCTTCCAG AAACATGGCA 
AAATATCGTA ATGTTCTCAA TAGTCAAAAT CTACAACCTC CCATAATCCC TCTATTCCCA 
GTTATCAAAA AGGATCTCAC CTTCCTTCAC GAAGGAAATG ACTCAAAAGT AGACGGGCTG 
GTCAATTTTG AGAAGCTAAG GATGATTGCA AAAGAAATTC GTCACGTTGG CCGAATGGCT 
TCAGTGAACA TGGACCCTGC CCTCATGTTC AGGACTCGGA AGAAGAAATG GCGGAGTTTG 
GGGTCTCTCA GCCAGGGTAG TACAAATGCA ACAGTGCTAG ATGTTGCTCA GACAGGTGGT 
CATAAAAAGC GGGTACGTCG TAGTTCCTTT CTCAATGCCA AAAAGCTTTA TGAAGATGCC 
CAAATGGCTC GAAAAGTGAA GCAGTACCTT TCCAATTTGG AGCTAGAAAT GGACGAGGAG 
AGTCTTCAGA CATTATCTCT GCAGTGTGAG CCAGCAACCA ACACATTGCC TAAGAATCCT 
GGTGACAAAA AGCCTGTCAA ATCCGAGACC TCTCCAGTAG CTCCAAGGGC AGGGTCACAA 
CAGAAAGCTC AGTCCCTGCC ACAGCCCCAG CAGCAGCCAC CACCAGCACA TAAAATCAAC 
CAGGGACTAC AGGTTCCCGC CGTGTCCCTT TATCCTTCAC GGAAGAAAGT GCCCGTAAAG 
GATCTCCCAC CTTTTGGCAT AAACTCTCCA CAAGCTTTAA AAAAAATTCT TTCTTTGTCT 
GAAGAAGGAA GTTTGGAACG TCACAAGAAA CAGGCTGAAG ATACAATATC AAATGCATCT 
TCGCAGCTTT CTTCTCCTCC TACTTCTCCA CAGAGTTCTC CAAGGAAAGG TGGCAGTGGC 
AATCAGCTGA GATCTTTTGG CTCCGGGCAG TTGGACTTAA CCAGTTCCTC CTCTTCTCTT 
GGAAGTGAGA ACAGTAACAA GAATAACAAT GCACCACGGA CCTATGGGAT AGGCTATACT 
TTGGCTCCCA GTGGTACTGT GGATAATTTT TCAGATTCTG GTCACAGTGA AATTTCTTCA 
CGATCCAGTA TTGTTAGCAA TTCGTCTTTT GACTCAGTGC CAGTCTCACT GCACGATGAG 
AGGCGCCAGA GGCATTCTGT CAGCATCGTG GAAACAAACC TAGGGATGGG CAGGATGGAG 
AGGCGGACCA TGATTGAACC TGATCAGTAT AGCTTGGGGT CCTATGCACC AATGTCCGAG 
GGCCGAGGCT TATATGCTAC AGCTACAGTA ATTTCTTCTC CAAGCACAGA GGAACTTTCC 
CAGGATCAGG GGGATCGCGC GTCACTTGAT GCTGCTGACA GTGGCCGTGG GAGCTGGACG 
TCATGCTCAA GTGGCTCCCA TGATAATATA CAGACGATCC AGCACCAGAG AAGCTGGGAG 
ACTCTTCCAT TCGGGCATAC TCACTTTGAT TATTCAGGGG ATCCTGCAGG TTTATGGGCA 
TCAAGCAGCC ATATGGACCA AATTATGTTT TCTGATCATA GCACAAAGTA TAACAGGCAA 
AATCAAAGTA GAGAGAGCCT TGAACAAGCC CAGTCCCGAG CAAGCTGGGC GTCTTCCACA 
GGTTACTGGG GAGAAGACTC AGAAGGTGAC ACAGGCACAA TAAAGCGGAG GGGTGGAAAG 
GATGTTTCCA TTGAAGCCGA AAGCAGTAGC CTAACGTCTG TGACTACGGA AGAAACCAAG 
CCTGTCCCCA TGCCTGCCCA CATAGCTGTG GCATCAAGTA CTACAAAGGG GCTCATTGCA 
CGAAAGGAGG GCAGGTATCG AGAGCCCCCG CCCACCCCTC CCGGCTACAT TGGAATTCCC 
ATTACTGACT TTCCAGAAGG GCACTCCCAT CCAGCCAGGA AACCGCCGGA CTACAACGTG 
GCCCTTCAGA GATCGCGGAT GGTCGCACGA TCCTCCGACA CAGCTGGGCC TTCATCCGTA 
CAGCAGCCAC ATGGGCATCC CACCAGCAGC AGGCCTGTGA ACAAACCTCA GTGGCATAAA 
CCGAACGAGT CTGACCCGCG CCTCGCCCCC TATCAGTCCC AAGGGTTTTC CACCGAGGAG 
GATGAAGATG AACAAGTTTC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011532427.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530729.1 Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.

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CloneID	OHu63163
Clone ID Related Accession (Same CDS sequence)	NM_001351728.1 , NM_001351728.2 , XM_011532427.2
Accession Version	XM_011532427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	rap guanine nucleotide exchange factor 2 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530729.1
CDS	995..5617
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPTALPVSGTLSSSNPDLLQSHHRILDFSATPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFAVTATPDQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLRSKTSCANLKRFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLELEMDEESLQTLSLQCEPATNTLPKNPGDKKPVKSETSPVAPRAGSQQKAQSLPQPQQQPPPAHKINQGLQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHKKQAEDTISNASSQLSSPPTSPQSSPRKGGSGNQLRSFGSGQLDLTSSSSSLGSENSNKNNNAPRTYGIGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVETNLGMGRMERRTMIEPDQYSLGSYAPMSEGRGLYATATVISSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPPPTPPGYIGIPITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011532427.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens Rap guanine nucleotide exchange factor 2 (RAPGEF2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532427.2

ORF Insert Sequence:

ATGAAACCAC TAGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA ACTGCATCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCTTCTA GCCATTCAGG ATGTAGTATC ACTAGTGATT CTGGGAGCAG CAGTCTTTCT 
GATATCTACC AGGCCACAGA AAGCGAGGCT GGTGATATGG ACCTGAGTGG GTTGCCAGAA 
ACAGCAGTGG ATTCCGAAGA CGACGACGAT GAAGAAGACA TTGAGAGAGC ATCAGATCCT 
CTGATGAGCA GGGACATTGT GAGAGACTGC CTAGAGAAGG ACCCAATTGA CCGGACAGAT 
GATGACATTG AACAACTCTT GGAATTTATG CACCAGTTGC CTGCTTTTGC CAATATGACA 
ATGTCAGTGA GGCGAGAACT CTGTGCTGTG ATGGTGTTCG CAGTGGTGGA AAGAGCAGGG 
ACCATAGTGT TAAATGATGG TGAAGAGCTG GACTCCTGGT CAGTGATTCT CAATGGATCT 
GTGGAAGTGA CTTATCCAGA TGGAAAAGCA GAAATACTGT GCATGGGAAA TAGTTTTGGT 
GTCTCTCCTA CCATGGACAA AGAATACATG AAAGGAGTGA TGAGAACAAA GGTGGATGAC 
TGCCAGTTTG TCTGCATAGC CCAGCAAGAT TACTGCCGTA TTCTCAATCA AGTAGAAAAG 
AACATGCAAA AAGTTGAAGA GGAAGGAGAG ATTGTTATGG TGAAAGAACA CCGAGAACTT 
GATCGAACTG GAACAAGAAA GGGACACATT GTCATCAAGG GTACCTCAGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA TTCAGTAGTA GATCCAACAT TCATAGAAGA CTTTCTGTTG 
ACCTATAGGA CTTTTCTTTC TAGCCCAATG GAAGTGGGCA AAAAGTTATT GGAGTGGTTT 
AATGACCCGA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TATTGTGGGT AAATAATCAC 
TTCAATGACT TTGAAGGAGA TCCTGCAATG ACTCGATTTT TAGAAGAATT TGAAAACAAT 
CTGGAAAGAG AGAAAATGGG TGGACACCTA AGGCTGTTGA ATATCGCGTG TGCTGCTAAA 
GCAAAAAGAA GATTGATGAC GTTAACAAAA CCATCCCGAG AAGCTCCTTT GCCTTTTATC 
TTACTTGGAG GCTCTGAGAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTCAGGTAGC 
AAAGCAACTG AAGCAGGCTT GAAACGGGGG GATCAGATAT TAGAAGTAAA TGGCCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCTATG GAAATTCTTA GAAATAACAC ACATTTATCT 
ATCACTGTGA AAACCAATTT ATTTGTATTT AAAGAACTTC TAACAAGATT GTCAGAAGAG 
AAAAGAAATG GTGCCCCCCA CCTTCCTAAA ATTGGTGACA TTAAAAAGGC CAGTCGCTAC 
TCCATTCCAG ATCTTGCTGT AGATGTAGAA CAGGTGATAG GACTTGAAAA AGTGAACAAA 
AAAAGTAAAG CCAACACTGT GGGAGGAAGG AACAAGCTGA AAAAGATACT CGACAAGACT 
CGGATCAGTA TCTTGCCACA GAAACCATAC AATGATATTG GGATTGGTCA GTCTCAAGAT 
GACAGCATAG TAGGATTAAG GCAGACAAAG CACATCCCAA CTGCATTGCC TGTCAGTGGA 
ACCTTATCAT CCAGTAATCC TGATTTATTG CAGTCACATC ATCGCATTTT AGACTTCAGT 
GCTACTCCTG ACTTGCCAGA TCAAGTGCTA AGGGTTTTTA AGGCTGATCA GCAAAGCCGC 
TACATCATGA TCAGTAAGGA CACTACAGCA AAGGAAGTGG TCATTCAGGC TATCAGGGAG 
TTTGCTGTTA CTGCCACCCC GGATCAATAT TCACTATGTG AGGTCTCTGT CACACCTGAG 
GGAGTAATCA AACAAAGAAG ACTTCCAGAT CAGCTTTCCA AACTTGCAGA CAGAATACAA 
CTGAGTGGAA GGTATTATCT GAAAAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAGG AGTTGTTGAG AGAGAGTCAA ATTTCCCTCC TTCAGCTCAG CACTGTGGAA 
GTTGCAACAC AGCTCTCTAT GCGAAATTTT GAACTCTTTC GCAACATTGA ACCTACTGAA 
TATATAGATG ATTTATTTAA ACTCAGATCA AAAACCAGCT GTGCCAACCT GAAGAGATTT 
GAAGAAGTCA TTAACCAGGA AACATTTTGG GTAGCATCTG AAATTCTCAG AGAAACAAAC 
CAGCTGAAGA GGATGAAGAT CATTAAGCAT TTCATCAAGA TAGCACTGCA CTGTAGGGAA 
TGCAAGAATT TTAACTCAAT GTTTGCAATC ATCAGTGGCC TAAACCTGGC ACCAGTGGCA 
AGACTGCGAA CGACCTGGGA GAAACTTCCC AATAAATACG AAAAACTATT TCAAGATCTC 
CAAGACCTGT TTGATCCTTC CAGAAACATG GCAAAATATC GTAATGTTCT CAATAGTCAA 
AATCTACAAC CTCCCATAAT CCCTCTATTC CCAGTTATCA AAAAGGATCT CACCTTCCTT 
CACGAAGGAA ATGACTCAAA AGTAGACGGG CTGGTCAATT TTGAGAAGCT AAGGATGATT 
GCAAAAGAAA TTCGTCACGT TGGCCGAATG GCTTCAGTGA ACATGGACCC TGCCCTCATG 
TTCAGGACTC GGAAGAAGAA ATGGCGGAGT TTGGGGTCTC TCAGCCAGGG TAGTACAAAT 
GCAACAGTGC TAGATGTTGC TCAGACAGGT GGTCATAAAA AGCGGGTACG TCGTAGTTCC 
TTTCTCAATG CCAAAAAGCT TTATGAAGAT GCCCAAATGG CTCGAAAAGT GAAGCAGTAC 
CTTTCCAATT TGGAGCTAGA AATGGACGAG GAGAGTCTTC AGACATTATC TCTGCAGTGT 
GAGCCAGCAA CCAACACATT GCCTAAGAAT CCTGGTGACA AAAAGCCTGT CAAATCCGAG 
ACCTCTCCAG TAGCTCCAAG GGCAGGGTCA CAACAGAAAG CTCAGTCCCT GCCACAGCCC 
CAGCAGCAGC CACCACCAGC ACATAAAATC AACCAGGGAC TACAGGTTCC CGCCGTGTCC 
CTTTATCCTT CACGGAAGAA AGTGCCCGTA AAGGATCTCC CACCTTTTGG CATAAACTCT 
CCACAAGCTT TAAAAAAAAT TCTTTCTTTG TCTGAAGAAG GAAGTTTGGA ACGTCACAAG 
AAACAGGCTG AAGATACAAT ATCAAATGCA TCTTCGCAGC TTTCTTCTCC TCCTACTTCT 
CCACAGAGTT CTCCAAGGAA AGGTGGCAGT GGCAATCAGC TGAGATCTTT TGGCTCCGGG 
CAGTTGGACT TAACCAGTTC CTCCTCTTCT CTTGGAAGTG AGAACAGTAA CAAGAATAAC 
AATGCACCAC GGACCTATGG GATAGGCTAT ACTTTGGCTC CCAGTGGTAC TGTGGATAAT 
TTTTCAGATT CTGGTCACAG TGAAATTTCT TCACGATCCA GTATTGTTAG CAATTCGTCT 
TTTGACTCAG TGCCAGTCTC ACTGCACGAT GAGAGGCGCC AGAGGCATTC TGTCAGCATC 
GTGGAAACAA ACCTAGGGAT GGGCAGGATG GAGAGGCGGA CCATGATTGA ACCTGATCAG 
TATAGCTTGG GGTCCTATGC ACCAATGTCC GAGGGCCGAG GCTTATATGC TACAGCTACA 
GTAATTTCTT CTCCAAGCAC AGAGGAACTT TCCCAGGATC AGGGGGATCG CGCGTCACTT 
GATGCTGCTG ACAGTGGCCG TGGGAGCTGG ACGTCATGCT CAAGTGGCTC CCATGATAAT 
ATACAGACGA TCCAGCACCA GAGAAGCTGG GAGACTCTTC CATTCGGGCA TACTCACTTT 
GATTATTCAG GGGATCCTGC AGGTTTATGG GCATCAAGCA GCCATATGGA CCAAATTATG 
TTTTCTGATC ATAGCACAAA GTATAACAGG CAAAATCAAA GTAGAGAGAG CCTTGAACAA 
GCCCAGTCCC GAGCAAGCTG GGCGTCTTCC ACAGGTTACT GGGGAGAAGA CTCAGAAGGT 
GACACAGGCA CAATAAAGCG GAGGGGTGGA AAGGATGTTT CCATTGAAGC CGAAAGCAGT 
AGCCTAACGT CTGTGACTAC GGAAGAAACC AAGCCTGTCC CCATGCCTGC CCACATAGCT 
GTGGCATCAA GTACTACAAA GGGGCTCATT GCACGAAAGG AGGGCAGGTA TCGAGAGCCC 
CCGCCCACCC CTCCCGGCTA CATTGGAATT CCCATTACTG ACTTTCCAGA AGGGCACTCC 
CATCCAGCCA GGAAACCGCC GGACTACAAC GTGGCCCTTC AGAGATCGCG GATGGTCGCA 
CGATCCTCCG ACACAGCTGG GCCTTCATCC GTACAGCAGC CACATGGGCA TCCCACCAGC 
AGCAGGCCTG TGAACAAACC TCAGTGGCAT AAACCGAACG AGTCTGACCC GCGCCTCGCC 
CCCTATCAGT CCCAAGGGTT TTCCACCGAG GAGGATGAAG ATGAACAAGT TTCTGCTGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substrate trapping proteomics reveals targets of the ?TrCP2/FBXW11 ubiquitin ligase.
Molecular and cellular biology35(1)167-81(2015 Jan)
Kim TY,Siesser PF,Rossman KL,Goldfarb D,Mackinnon K,Yan F,Yi X,MacCoss MJ,Moon RT,Der CJ,Major MB

Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex.
Nature communications54826(2014 Sep)
Ye T,Ip JP,Fu AK,Ip NY

The PDZ domain of the guanine nucleotide exchange factor PDZGEF directs binding to phosphatidic acid during brush border formation.
PloS one9(5)e98253(2014)
Consonni SV,Brouwer PM,van Slobbe ES,Bos JL

Control of epithelial cell migration and invasion by the IKK?- and CK1?-mediated degradation of RAPGEF2.
Developmental cell27(5)574-85(2013 Dec)
Magliozzi R,Low TY,Weijts BG,Cheng T,Spanjaard E,Mohammed S,van Veen A,Ovaa H,de Rooij J,Zwartkruis FJ,Bos JL,de Bruin A,Heck AJ,Guardavaccaro D

NEDD4-1 regulates migration and invasion of glioma cells through CNrasGEF ubiquitination in vitro.
PloS one8(12)e82789(2013)
Zhang H,Nie W,Zhang X,Zhang G,Li Z,Wu H,Shi Q,Chen Y,Ding Z,Zhou X,Yu R

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RAPGEF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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