DEPDC5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEPDC5
Entrez Gene ID	9681
Full Name	DEP domain containing 5
Synonyms	DEP.5,FFEVF,FFEVF1
General protein information	Preferred Names DEP domain containing 5 Names GATOR complex protein DEPDC5 DEP domain-containing protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.2-q12.3
Summary	This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014].
Disorder MIM:	614191
Disorder Html:	Epilepsy, familial focal, with variable foci 1, 604364 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005261862.1	XP_005261919.1	DEP domain-containing protein 5 isoform X1
NM_001363852.1	NP_001350781.1	GATOR complex protein DEPDC5 isoform 6
XM_011530565.2	XP_011528867.1	GATOR complex protein DEPDC5 isoform X9
XM_011530568.2	XP_011528870.1	GATOR complex protein DEPDC5 isoform X10
XM_017029113.1	XP_016884602.1	GATOR complex protein DEPDC5 isoform X13
NM_001369902.1	NP_001356831.1	GATOR complex protein DEPDC5 isoform 8
NM_001364318.1	NP_001351247.1	GATOR complex protein DEPDC5 isoform 4
XM_011530559.2	XP_011528861.1	GATOR complex protein DEPDC5 isoform X4
NM_001242896.2	NP_001229825.1	GATOR complex protein DEPDC5 isoform 4
NM_014662.4	NP_055477.1	GATOR complex protein DEPDC5 isoform 1
NM_001136029.2	NP_001129501.1	GATOR complex protein DEPDC5 isoform 3
NM_001007188.2	NP_001007189.1	GATOR complex protein DEPDC5 isoform 2
NM_001242897.1	NP_001229826.1	GATOR complex protein DEPDC5 isoform 5
NM_001363854.1	NP_001350783.1	GATOR complex protein DEPDC5 isoform 7
NM_014662.5	NP_055477.1	GATOR complex protein DEPDC5 isoform 1
NM_001364319.1	NP_001351248.1	GATOR complex protein DEPDC5 isoform 7
NM_001363852.2	NP_001350781.1	GATOR complex protein DEPDC5 isoform 6
XM_011530561.2	XP_011528863.1	GATOR complex protein DEPDC5 isoform X6
XM_011530562.2	XP_011528864.1	GATOR complex protein DEPDC5 isoform X7
XM_017029112.1	XP_016884601.1	DEP domain-containing protein 5 isoform X5
XM_011530558.2	XP_011528860.1	DEP domain-containing protein 5 isoform X3
NM_001364320.1	NP_001351249.1	GATOR complex protein DEPDC5 isoform 6
NM_001242897.2	NP_001229826.1	GATOR complex protein DEPDC5 isoform 5
NM_001007188.3	NP_001007189.1	GATOR complex protein DEPDC5 isoform 2
NM_001242896.3	NP_001229825.1	GATOR complex protein DEPDC5 isoform 4
XM_011530569.2	XP_011528871.1	GATOR complex protein DEPDC5 isoform X11
NM_001242896.1	NP_001229825.1	GATOR complex protein DEPDC5 isoform 4
XM_017029110.1	XP_016884599.1	DEP domain-containing protein 5 isoform X3
XM_017029109.1	XP_016884598.1	DEP domain-containing protein 5 isoform X2
NM_001369903.1	NP_001356832.1	GATOR complex protein DEPDC5 isoform 1
XM_024452305.1	XP_024308073.1	GATOR complex protein DEPDC5 isoform X12
XM_017029114.1	XP_016884603.1	GATOR complex protein DEPDC5 isoform X14
XM_011530560.2	XP_011528862.1	DEP domain-containing protein 5 isoform X5
XM_017029111.1	XP_016884600.1	DEP domain-containing protein 5 isoform X4
XM_011530563.2	XP_011528865.1	GATOR complex protein DEPDC5 isoform X8
XM_017029115.1	XP_016884604.1	GATOR complex protein DEPDC5 isoform X15
NM_001136029.3	NP_001129501.1	GATOR complex protein DEPDC5 isoform 3
NM_001369901.1	NP_001356830.1	GATOR complex protein DEPDC5 isoform 8
NM_001363854.2	NP_001350783.1	GATOR complex protein DEPDC5 isoform 7
XM_011530557.2	XP_011528859.1	GATOR complex protein DEPDC5 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DEPDC5	XP_415249.4
Mus musculus (house mouse)	Depdc5	NP_001164038.1
Rattus norvegicus (Norway rat)	Depdc5	NP_001100699.1
Homo sapiens (human)	DEPDC5	NP_001229825.1
Canis lupus familiaris (dog)	DEPDC5	XP_005636547.1
Bos taurus (cattle)	LOC521542	XP_005218248.1
Xenopus tropicalis (tropical clawed frog)	LOC100498286	XP_004910648.1
Pan troglodytes (chimpanzee)	DEPDC5	XP_003317262.1
Danio rerio (zebrafish)	depdc5	XP_005155706.1
Drosophila melanogaster (fruit fly)	CG12090	NP_001261260.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007010	XP_308760.4

Gene Ontology
Bibliography

Related articles in PubMed

DEPDC5 deficiency contributes to resistance to leucine starvation via p62 accumulation in hepatocellular carcinoma.
Mizuno Y, Shimada S, Akiyama Y, Watanabe S, Aida T, Ogawa K, Ono H, Mitsunori Y, Ban D, Kudo A, Arii S, Yamaoka S, Tanabe M, Tanaka S
Scientific reports8(1)106(2018 Jan)

KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
Wolfson RL, Chantranupong L, Wyant GA, Gu X, Orozco JM, Shen K, Condon KJ, Petri S, Kedir J, Scaria SM, Abu-Remaileh M, Frankel WN, Sabatini DM
Nature543(7645)438-442(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This cohort study showed DEPDC5 mutations were present in 8% of individuals with focal epilepsy, including one individual with focal cortical dysplasia.
Title: Involvement of GATOR complex genes in familial focal epilepsies and focal cortical dysplasia.

DEPDC5 variants increase fibrosis progression in European subjects with chronic HCV infection. Our findings suggest that DEPDC5 down-regulation may contribute to HCV-related fibrosis by increasing MMP2 synthesis through the beta-catenin pathway
Title: DEPDC5 variants increase fibrosis progression in Europeans with chronic hepatitis C virus infection.

This study found a single DEPDC5 mutation in one of (2.2%) 45 families with genetic temporal lobe epilepsy, a proportion much lower than that reported in other inherited focal epilepsies.
Title: DEPDC5 mutations are not a frequent cause of familial temporal lobe epilepsy.

This revealed four patients to have two or more tumors that were clonally related, all of which lacked MED12 mutations. DEPDC5 was discovered as a novel tumor suppressor gene playing a role in the progression of uterine leiomyomas.
Title: Clonally related uterine leiomyomas are common and display branched tumor evolution.

MICA and DEPDC5 SNPs were found to be strongly associated with HCV-induced HCC.
Title: [Hepatitis C virus induced hepatocellular carcinoma associated genes].

The following DEPDC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEPDC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16797	NM_001364318.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu16797	NM_001242896.3 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu16797	XM_005261862.1 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu16900	NM_001007188.3 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu63150	XM_011530569.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu105284	XM_024452305.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu93664	XM_017029113.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu93665	XM_017029114.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu93666	XM_017029115.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu53772	XM_011530565.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu53775	XM_011530568.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu108683	NM_001369902.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108683	NM_001369901.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu19567	NM_001242897.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu22513	NM_001136029.3 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu23642	NM_001369903.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu23642	NM_014662.5 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53768	NM_001364320.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53768	NM_001363852.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53769	NM_001364319.1 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53769	NM_001363854.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu19567	XM_011530563.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu22513	XM_017029109.1 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu22513	XM_011530557.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu23642	XM_011530559.2 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu23642	XM_017029111.1 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53768	XM_017029110.1 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53768	XM_011530558.2 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53769	XM_017029112.1 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53769	XM_011530560.2 Latest version!	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53770	XM_011530561.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu63148	XM_011530562.2 Latest version!	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu23642	NM_014662.4	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53768	NM_001363852.1	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu19567	NM_001242897.1	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu16900	NM_001007188.2	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu16797	NM_001242896.2	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu16797	NM_001242896.1	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu22513	NM_001136029.2	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu53769	NM_001363854.1	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEPDC5 ( NM_001364318.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351247.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 11, mRNA.

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CloneID	OHu16797
Clone ID Related Accession (Same CDS sequence)	XM_005261862.1 , NM_001364318.1 , NM_001242896.2 , NM_001242896.3 , NM_001242896.1
Accession Version	NM_001364318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Jun 16, 2018 this sequence version replaced XM_005261862.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.234626.1, SRR1803611.177876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351247.1
CDS	90..4901
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001364318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364318.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001242896.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229825.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.

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CloneID	OHu16797
Clone ID Related Accession (Same CDS sequence)	XM_005261862.1 , NM_001364318.1 , NM_001242896.2 , NM_001242896.3 , NM_001242896.1
Accession Version	NM_001242896.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, BP354669.1, BC136612.1 and DB381506.1. On Jun 16, 2018 this sequence version replaced NM_001242896.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.151427.1, BC136612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229825.1
CDS	207..5018
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001242896.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242896.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001242896.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229825.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 4, mRNA.

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CloneID	OHu16797
Clone ID Related Accession (Same CDS sequence)	XM_005261862.1 , NM_001364318.1 , NM_001242896.2 , NM_001242896.3 , NM_001242896.1
Accession Version	NM_001242896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, BP354669.1, BC136612.1, DB381506.1 and Z83856.2. On Aug 31, 2019 this sequence version replaced NM_001242896.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136612.1, SRR1803613.151427.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229825.1
CDS	207..5018
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001242896.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242896.3

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001242896.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229825.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.

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CloneID	OHu16797
Clone ID Related Accession (Same CDS sequence)	XM_005261862.1 , NM_001364318.1 , NM_001242896.2 , NM_001242896.3 , NM_001242896.1
Accession Version	NM_001242896.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP354669.1, BC136612.1 and DB381506.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.151427.1, BC136612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229825.1
CDS	203..5014
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001242896.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242896.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_005261862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261919.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA.

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CloneID	OHu16797
Clone ID Related Accession (Same CDS sequence)	XM_005261862.1 , NM_001364318.1 , NM_001242896.2 , NM_001242896.3 , NM_001242896.1
Accession Version	XM_005261862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261919.1
CDS	107..4918
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_005261862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261862.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001007188.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007189.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16900
Clone ID Related Accession (Same CDS sequence)	NM_001007188.2 , NM_001007188.3
Accession Version	NM_001007188.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP354669.1, BC057797.1 and CA310381.1. On Jul 2, 2011 this sequence version replaced NM_001007188.1. Transcript Variant: This variant (2) lacks a large portion of the 3' coding region and has a different 3' structure compared to variant 4. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.242109.1, SRR1803614.142704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGAGCA CCTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007189.1
CDS	203..1882
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTREHLG

Target ORF information:

RefSeq Version	NM_001007188.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007188.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGAGCA CCTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001007188.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001007189.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 2, mRNA.

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CloneID	OHu16900
Clone ID Related Accession (Same CDS sequence)	NM_001007188.2 , NM_001007188.3
Accession Version	NM_001007188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, BP354669.1, BC057797.1 and CA310381.1. On Jun 15, 2018 this sequence version replaced NM_001007188.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.242109.1, SRR1803614.142704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGAGCA CCTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007189.1
CDS	207..1886
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTREHLG

Target ORF information:

RefSeq Version	NM_001007188.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007188.3

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGAGCA CCTAGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530569.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528871.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X20, mRNA.

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CloneID	OHu63150
Clone ID Related Accession (Same CDS sequence)	XM_011530569.2
Accession Version	XM_011530569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTA GGACCCAGAA TAAGGATTCT CTAGAGGACA GTGTTTCTAC CTCTCCAGAC 
CCAATTCTGA CACTGTCTGC TCCCCCTGTA GTGCCAGGCT TCTGTTGCAC AGTTGGAGTG 
GACTGGAAGT CTCTCACTAC TCCGGCGTGC CTCCCCCTTA CCACCGACTA CTTCCCTGAC 
CGCCAGGGCC TGCAGAATGA CTACACAGAG GGCTGTTATG ATCTCCTTCC AGAAGCAGAC 
ATCGACAGGA GGGACGAAGA TGGTGTGCAG ATGACAGCCC AGCAGGTATT TGAAGAGTTT 
ATTTGCCAAC GTCTCATGCA GGGCTACCAA ATCATAGTGC AGCCCAAGAC ACAGAAACCC 
AATCCTGCTG TCCCGCCCCC GCTGAGCAGT AGCCCACTCT ATAGCCGAGG CCTTGTGTCC 
CGAAACCGCC CTGAGGAGGA GGACCAGTAT TGGCTGAGTA TGGGCAGAAC GTTCCACAAA 
GTGACGCTGA AGGATAAGAT GATCACAGTG ACGCGATACC TTCCCAAGTA TCCTTATGAA 
TCTGCCCAGA TCCACTACAC CTACAGCCTC TGTCCTTCCC ACTCAGACTC AGAGTTCGTC 
TCCTGCTGGG TGGAATTCTC CCACGAACGG CTGGAGGAGT ACAAGTGGAA TTACTTAGAT 
CAGTATATCT GTTCTGCCGG CTCTGAAGAC TTCAGCTTAA TTGAGTCCCT GAAGTTCTGG 
AGGACCCGCT TCCTGCTGCT GCCAGCCTGT GTCACCGCCA CCAAGCGCAT CACGGAGGGG 
GAGGCCCACT GCGACATCTA TGGGGACAGG CCCCGTGCAG ACGAGGACGA GTGGCAACTC 
CTGGATGGTT TTGTCCGCTT TGTGGAGGGC TTGAATCGCA TTCGCAGGCG GCATCGCTCG 
GATCGCATGA TGCGGAAAGG GACCGCCATG AAAGGCTTGC AGATGACTGG GCCCATTTCC 
ACGCATTCTC TGGAGTCAAC TGCACCCCCA GTGGGGAAGA AGGGAACCTC AGCTCTCTCT 
GCCCTGTTGG AGATGGAGGC CAGTCAGAAG TGCCTGGGAG AACAGCAGGC AGCTGTGCAT 
GGTGGGAAGA GCTCCGCCCA GTCAGCCGAG AGCAGCAGCG TTGCCATGAC TCCCACCTAC 
ATGGACAGCC CACGAAAGGA CGGGGCCTTC TTTATGGAGT TTGTCCGCAG CCCACGCACA 
GCATCGTCCG CCTTCTACCC TCAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528871.1
CDS	80..2785
Translation	MNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530569.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530569.2

ORF Insert Sequence:

ATGAATCCTA GGACCCAGAA TAAGGATTCT CTAGAGGACA GTGTTTCTAC CTCTCCAGAC 
CCAATTCTGA CACTGTCTGC TCCCCCTGTA GTGCCAGGCT TCTGTTGCAC AGTTGGAGTG 
GACTGGAAGT CTCTCACTAC TCCGGCGTGC CTCCCCCTTA CCACCGACTA CTTCCCTGAC 
CGCCAGGGCC TGCAGAATGA CTACACAGAG GGCTGTTATG ATCTCCTTCC AGAAGCAGAC 
ATCGACAGGA GGGACGAAGA TGGTGTGCAG ATGACAGCCC AGCAGGTATT TGAAGAGTTT 
ATTTGCCAAC GTCTCATGCA GGGCTACCAA ATCATAGTGC AGCCCAAGAC ACAGAAACCC 
AATCCTGCTG TCCCGCCCCC GCTGAGCAGT AGCCCACTCT ATAGCCGAGG CCTTGTGTCC 
CGAAACCGCC CTGAGGAGGA GGACCAGTAT TGGCTGAGTA TGGGCAGAAC GTTCCACAAA 
GTGACGCTGA AGGATAAGAT GATCACAGTG ACGCGATACC TTCCCAAGTA TCCTTATGAA 
TCTGCCCAGA TCCACTACAC CTACAGCCTC TGTCCTTCCC ACTCAGACTC AGAGTTCGTC 
TCCTGCTGGG TGGAATTCTC CCACGAACGG CTGGAGGAGT ACAAGTGGAA TTACTTAGAT 
CAGTATATCT GTTCTGCCGG CTCTGAAGAC TTCAGCTTAA TTGAGTCCCT GAAGTTCTGG 
AGGACCCGCT TCCTGCTGCT GCCAGCCTGT GTCACCGCCA CCAAGCGCAT CACGGAGGGG 
GAGGCCCACT GCGACATCTA TGGGGACAGG CCCCGTGCAG ACGAGGACGA GTGGCAACTC 
CTGGATGGTT TTGTCCGCTT TGTGGAGGGC TTGAATCGCA TTCGCAGGCG GCATCGCTCG 
GATCGCATGA TGCGGAAAGG GACCGCCATG AAAGGCTTGC AGATGACTGG GCCCATTTCC 
ACGCATTCTC TGGAGTCAAC TGCACCCCCA GTGGGGAAGA AGGGAACCTC AGCTCTCTCT 
GCCCTGTTGG AGATGGAGGC CAGTCAGAAG TGCCTGGGAG AACAGCAGGC AGCTGTGCAT 
GGTGGGAAGA GCTCCGCCCA GTCAGCCGAG AGCAGCAGCG TTGCCATGAC TCCCACCTAC 
ATGGACAGCC CACGAAAGGA CGGGGCCTTC TTTATGGAGT TTGTCCGCAG CCCACGCACA 
GCATCGTCCG CCTTCTACCC TCAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_024452305.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308073.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu105284
Clone ID Related Accession (Same CDS sequence)	XM_024452305.1
Accession Version	XM_024452305.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCCTA GGACCCAGAA TAAGGATTCT CTAGAGGACA GTGTTTCTAC CTCTCCAGAC 
CCAATGCCAG GCTTCTGTTG CACAGTTGGA GTGGACTGGA AGTCTCTCAC TACTCCGGCG 
TGCCTCCCCC TTACCACCGA CTACTTCCCT GACCGCCAGG GCCTGCAGAA TGACTACACA 
GAGGGCTGTT ATGATCTCCT TCCAGAAGCA GACATCGACA GGAGGGACGA AGATGGTGTG 
CAGATGACAG CCCAGCAGGT ATTTGAAGAG TTTATTTGCC AACGTCTCAT GCAGGGCTAC 
CAAATCATAG TGCAGCCCAA GACACAGAAA CCCAATCCTG CTGTCCCGCC CCCGCTGAGC 
AGTAGCCCAC TCTATAGCCG AGGCCTTGTG TCCCGAAACC GCCCTGAGGA GGAGGACCAG 
TATTGGCTGA GTATGGGCAG AACGTTCCAC AAAGTGACGC TGAAGGATAA GATGATCACA 
GTGACGCGAT ACCTTCCCAA GTATCCTTAT GAATCTGCCC AGATCCACTA CACCTACAGC 
CTCTGTCCTT CCCACTCAGA CTCAGAGTTC GTCTCCTGCT GGGTGGAATT CTCCCACGAA 
CGGCTGGAGG AGTACAAGTG GAATTACTTA GATCAGTATA TCTGTTCTGC CGGCTCTGAA 
GACTTCAGCT TAATTGAGTC CCTGAAGTTC TGGAGGACCC GCTTCCTGCT GCTGCCAGCC 
TGTGTCACCG CCACCAAGCG CATCACGGAG GGGGAGGCCC ACTGCGACAT CTATGGGGAC 
AGGCCCCGTG CAGACGAGGA CGAGTGGCAA CTCCTGGATG GTTTTGTCCG CTTTGTGGAG 
GGCTTGAATC GCATTCGCAG GCGGCATCGC TCGGATCGCA TGATGCGGAA AGGGACCGCC 
ATGAAAGGCT TGCAGATGAC TGGGCCCATT TCCACGCATT CTCTGGAGTC AACTGCACCC 
CCAGTGGGGA AGAAGGGAAC CTCAGCTCTC TCTGCCCTGT TGGAGATGGA GGCCAGTCAG 
AAGTGCCTGG GAGAACAGCA GGCAGCTGTG CATGGTGGGA AGAGCTCCGC CCAGTCAGCC 
GAGAGCAGCA GCGTTGCCAT GACTCCCACC TACATGGACA GCCCACGAAA GGACGGGGCC 
TTCTTTATGG AGTTTGTCCG CAGCCCACGC ACAGCATCGT CCGCCTTCTA CCCTCAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308073.1
CDS	79..2757
Translation	MNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_024452305.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452305.1

ORF Insert Sequence:

ATGAATCCTA GGACCCAGAA TAAGGATTCT CTAGAGGACA GTGTTTCTAC CTCTCCAGAC 
CCAATGCCAG GCTTCTGTTG CACAGTTGGA GTGGACTGGA AGTCTCTCAC TACTCCGGCG 
TGCCTCCCCC TTACCACCGA CTACTTCCCT GACCGCCAGG GCCTGCAGAA TGACTACACA 
GAGGGCTGTT ATGATCTCCT TCCAGAAGCA GACATCGACA GGAGGGACGA AGATGGTGTG 
CAGATGACAG CCCAGCAGGT ATTTGAAGAG TTTATTTGCC AACGTCTCAT GCAGGGCTAC 
CAAATCATAG TGCAGCCCAA GACACAGAAA CCCAATCCTG CTGTCCCGCC CCCGCTGAGC 
AGTAGCCCAC TCTATAGCCG AGGCCTTGTG TCCCGAAACC GCCCTGAGGA GGAGGACCAG 
TATTGGCTGA GTATGGGCAG AACGTTCCAC AAAGTGACGC TGAAGGATAA GATGATCACA 
GTGACGCGAT ACCTTCCCAA GTATCCTTAT GAATCTGCCC AGATCCACTA CACCTACAGC 
CTCTGTCCTT CCCACTCAGA CTCAGAGTTC GTCTCCTGCT GGGTGGAATT CTCCCACGAA 
CGGCTGGAGG AGTACAAGTG GAATTACTTA GATCAGTATA TCTGTTCTGC CGGCTCTGAA 
GACTTCAGCT TAATTGAGTC CCTGAAGTTC TGGAGGACCC GCTTCCTGCT GCTGCCAGCC 
TGTGTCACCG CCACCAAGCG CATCACGGAG GGGGAGGCCC ACTGCGACAT CTATGGGGAC 
AGGCCCCGTG CAGACGAGGA CGAGTGGCAA CTCCTGGATG GTTTTGTCCG CTTTGTGGAG 
GGCTTGAATC GCATTCGCAG GCGGCATCGC TCGGATCGCA TGATGCGGAA AGGGACCGCC 
ATGAAAGGCT TGCAGATGAC TGGGCCCATT TCCACGCATT CTCTGGAGTC AACTGCACCC 
CCAGTGGGGA AGAAGGGAAC CTCAGCTCTC TCTGCCCTGT TGGAGATGGA GGCCAGTCAG 
AAGTGCCTGG GAGAACAGCA GGCAGCTGTG CATGGTGGGA AGAGCTCCGC CCAGTCAGCC 
GAGAGCAGCA GCGTTGCCAT GACTCCCACC TACATGGACA GCCCACGAAA GGACGGGGCC 
TTCTTTATGG AGTTTGTCCG CAGCCCACGC ACAGCATCGT CCGCCTTCTA CCCTCAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029113.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884602.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu93664
Clone ID Related Accession (Same CDS sequence)	XM_017029113.1
Accession Version	XM_017029113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA GGCCTGCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884602.1
CDS	210..2393
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPRPAE

Target ORF information:

RefSeq Version	XM_017029113.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029113.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA GGCCTGCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029114.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884603.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu93665
Clone ID Related Accession (Same CDS sequence)	XM_017029114.1
Accession Version	XM_017029114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGAAAATT TAGCTCCAAG TGGCTTTTGG CTATTGACAA AGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884603.1
CDS	210..2360
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAENLAPSGFWLLTKNR

Target ORF information:

RefSeq Version	XM_017029114.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029114.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGAAAATT TAGCTCCAAG TGGCTTTTGG CTATTGACAA AGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029115.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884604.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu93666
Clone ID Related Accession (Same CDS sequence)	XM_017029115.1
Accession Version	XM_017029115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAAGGCC TGCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884604.1
CDS	210..2159
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPRPAE

Target ORF information:

RefSeq Version	XM_017029115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029115.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAAGGCC TGCAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530565.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528867.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu53772
Clone ID Related Accession (Same CDS sequence)	XM_011530565.2
Accession Version	XM_011530565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGTGGC CATGCAGCAG CCCGCCACCA 
CCTGGCACAC AGCAGGAGTG GACGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG 
CCTTTGTGGC AGAAGAGCTC GTGCACTCTG AGATTCCTGC CTTTCTCCTG CCCTGGCTGC 
CTAGCCGGCC AGCCTCCTAT GCAAGTAGGC ACAGCTCCTT TAGCCGAAGT TTTGGAGGAC 
GGAGCCAGGC GGCAGCACTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528867.1
CDS	210..4133
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQWPCSSPPPPGTQQEWTTSPASSASGLRWPLWQKSSCTLRFLPFSCPGCLAGQPPMQVGTAPLAEVLEDGARRQHF

Target ORF information:

RefSeq Version	XM_011530565.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530565.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGTGGC CATGCAGCAG CCCGCCACCA 
CCTGGCACAC AGCAGGAGTG GACGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG 
CCTTTGTGGC AGAAGAGCTC GTGCACTCTG AGATTCCTGC CTTTCTCCTG CCCTGGCTGC 
CTAGCCGGCC AGCCTCCTAT GCAAGTAGGC ACAGCTCCTT TAGCCGAAGT TTTGGAGGAC 
GGAGCCAGGC GGCAGCACTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530568.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528870.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X19, mRNA.

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CloneID	OHu53775
Clone ID Related Accession (Same CDS sequence)	XM_011530568.2
Accession Version	XM_011530568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGG 
AAGTGGAGAC TGTTCCTAAT ATTCGTGCTC TTGACTGCAT ACGTGCCATC TTGCTTTGCT 
GACCTGACAC CCTGTGTTAC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528870.1
CDS	210..3740
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSRKWRLFLIFVLLTAYVPSCFADLTPCVTALSSWWQAP

Target ORF information:

RefSeq Version	XM_011530568.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530568.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGG 
AAGTGGAGAC TGTTCCTAAT ATTCGTGCTC TTGACTGCAT ACGTGCCATC TTGCTTTGCT 
GACCTGACAC CCTGTGTTAC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001369902.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356831.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu108683
Clone ID Related Accession (Same CDS sequence)	NM_001369902.1 , NM_001369901.1
Accession Version	NM_001369902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.80139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG TCAGCACATG TGCCTATATC 
ACCCAGAAGG TGGAGTTTGC TGGCATCAGA GCACAGGCTG GTGAACTGTG GGTTAAGAAT 
GAGAAGGTCA TGTGTGGCTA CATCAGTGAA GATACCAGGG TGGTGTTTCG TTCTACGTCG 
GCTATGGTTT ACATATTTAT TCAGATGAGC TGTGAAATGT GGGATTTTGA TATTTATGGG 
GATTTGTATT TTGAGAAAGC TGTGAATGGT TTCCTTGCTG ATCTATTTAC CAAGTGGAAG 
GAGAAGAACT GTAGTCATGA AGTGACAGTG GTCCTGTTTT CTAGAACTTT CTATGATGCA 
AAATCTGTTG ATGAATTTCC TGAAATAAAC CGAGCCTCAA TTCGACAGGA TCACAAGGGG 
AGATTCTATG AAGACTTTTA CAAAGTGGTG GTGCAGAATG AGAGAAGAGA AGAATGGACT 
TCACTTCTCG TAACCATTAA AAAACTCTTC ATCCAGTATC CAGTGTTGGT GCGACTGGAA 
CAGGCAGAGG GCTTTCCTCA AGGAGATAAT TCTACCTCAG CACAAGGAAA CTACCTGGAG 
GCCATCAATC TGTCATTCAA TGTGTTTGAT AAGCACTACA TCAACCGCAA CTTTGACCGA 
ACTGGGCAGA TGTCAGTGGT GATCACGCCC GGGGTGGGTG TCTTTGAAGT GGACCGCCTA 
CTCATGATCC TGACCAAGCA GCGGATGATA GATAATGGAA TTGGTGTGGA TTTGGTGTGC 
ATGGGAGAGC AACCGTTACA TGCTGTCCCA TTGTTCAAGC TCCATAATCG GAGTGCTCCC 
CGTGATTCTC GTCTGGGCGA TGACTATAAT ATCCCTCACT GGATAAACCA CAGTTTCTAC 
ACATCCAAAA GCCAGCTCTT TTGTAATAGT TTCACCCCAC GAATAAAACT GGCAGGAAAG 
AAGCCCGCCT CTGAGAAAGC AAAAAATGGC CGTGATACAT CTCTCGGGAG TCCAAAAGAA 
TCTGAGAACG CCCTTCCCAT CCAAGTAGAT TATGACGCCT ATGACGCTCA AGTGTTCAGG 
CTGCCCGGCC CATCCCGGGC CCAGTGCCTC ACCACCTGCA GATCTGTGCG AGAGCGAGAG 
AGTCACAGTC GAAAGAGTGC CAGCTCCTGT GATGTTTCAT CCAGCCCTTC CCTACCAAGC 
CGCACACTGC CCACTGAGGA AGTGAGGAGC CAGGCTTCTG ACGACAGCTC CCTAGGCAAG 
AGTGCCAACA TCCTGATGAT CCCACACCCC CACCTGCACC AGTATGAAGT CAGCAGCTCC 
TTGGGATACA CCAGCACTCG AGATGTCCTG GAGAACATGA TGGAGCCACC ACAGCGAGAC 
TCCAGTGCAC CAGGGAGGTT TCACGTTGGC AGTGCAGAAT CCATGCTGCA TGTTCGACCT 
GGTGGATACA CGCCCCAGAG AGCACTGATT AACCCCTTCG CTCCCTCTCG GATGCCCATG 
AAGCTTACGT CCAACAGAAG GCGCTGGATG CACACTTTTC CTGTGGGGCC ATCCGGAGAA 
GCCATCCAGA TCCACCACCA GACCCGACAG AATATGGCGG AGCTACAAGG CAGCGGGCAG 
AGGGATCCAA CTCACTCCTC TGCAGAGCTG CTGGAGTTAG CATATCATGA AGCTGCTGGA 
AGGCACAGCA ATTCCCGCCA GCCTGGTGAC GGCATGTCCT TCTTGAACTT CAGTGGAACA 
GAGGAGCTTT CTGTCGGCCT GCTTAGCAAC AGTGGTGCAG GTATGAATCC TAGGACCCAG 
AATAAGGATT CTCTAGAGGA CAGTGTTTCT ACCTCTCCAG ACCCAATTCT GACACTGTCT 
GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC ACAGTTGGAG TGGACTGGAA GTCTCTCACT 
ACTCCGGCGT GCCTCCCCCT TACCACCGAC TACTTCCCTG ACCGCCAGGG CCTGCAGAAT 
GACTACACAG AGGGCTGTTA TGATCTCCTT CCAGAAGCAG ACATCGACAG GAGGGACGAA 
GATGGTGTGC AGATGACAGC CCAGCAGGTA TTTGAAGAGT TTATTTGCCA ACGTCTCATG 
CAGGGCTACC AAATCATAGT GCAGCCCAAG ACACAGAAAC CCAATCCTGC TGTCCCGCCC 
CCGCTGAGCA GTAGCCCACT CTATAGCCGA GGCCTTGTGT CCCGAAACCG CCCTGAGGAG 
GAGGACCAGT ATTGGCTGAG TATGGGCAGA ACGTTCCACA AAGTGACGCT GAAGGATAAG 
ATGATCACAG TGACGCGATA CCTTCCCAAG TATCCTTATG AATCTGCCCA GATCCACTAC 
ACCTACAGCC TCTGTCCTTC CCACTCAGAC TCAGAGTTCG TCTCCTGCTG GGTGGAATTC 
TCCCACGAAC GGCTGGAGGA GTACAAGTGG AATTACTTAG ATCAGTATAT CTGTTCTGCC 
GGCTCTGAAG ACTTCAGCTT AATTGAGTCC CTGAAGTTCT GGAGGACCCG CTTCCTGCTG 
CTGCCAGCCT GTGTCACCGC CACCAAGCGC ATCACGGAGG GGGAGGCCCA CTGCGACATC 
TATGGGGACA GGCCCCGTGC AGACGAGGAC GAGTGGCAAC TCCTGGATGG TTTTGTCCGC 
TTTGTGGAGG GCTTGAATCG CATTCGCAGG CGGCATCGCT CGGATCGCAT GATGCGGAAA 
GGGACCGCCA TGAAAGGCTT GCAGATGACT GGGCCCATTT CCACGCATTC TCTGGAGTCA 
ACTGCACCCC CAGTGGGGAA GAAGGGAACC TCAGCTCTCT CTGCCCTGTT GGAGATGGAG 
GCCAGTCAGA AGTGCCTGGG AGAACAGCAG GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC 
CAGTCAGCCG AGAGCAGCAG CGTTGCCATG ACTCCCACCT ACATGGACAG CCCACGAAAG 
GACGGGGCCT TCTTTATGGA GTTTGTCCGC AGCCCACGCA CAGCATCGTC CGCCTTCTAC 
CCTCAGGTAT CTGTGGACCA AACAGCCACT CCTATGTTGG ACGGCACCAG TTTGGGCATA 
TGCACAGGCC AATCCATGGA CAGAGGCAAC AGCCAGACCT TTGGGAACTC CCAGAACATA 
GGAGAACAGG GCTACTCCTC CACAAACTCC AGTGACAGCA GCTCTCAGCA GCTGGTGGCA 
AGCTCCTTGA CCTCATCCTC TACCCTGACA GAGATCCTGG AAGCCATGAA GCACCCCTCG 
ACAGGAGTCC AGCTGCTCTC TGAACAGAAG GGCCTCTCAC CGTACTGCTT CATCAGCGCG 
GAGGTGGTAC ACTGGTTGGT GAACCACGTG GAGGGGATCC AGACACAGGC GATGGCCATT 
GACATCATGC AGAAAATGCT GGAAGAGCAG CTCATCACAC ATGCATCTGG CGAAGCCTGG 
CGGACCTTCA TCTACGGCTT CTATTTCTAC AAGATAGTAA CGGACAAAGA GCCCGACCGA 
GTGGCCATGC AGCAGCCCGC CACCACCTGG CACACAGCAG GAGTGGACGA CTTCGCCAGC 
TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCAGAAG AGCTCGTGCA CTCTGAGATT 
CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC CGGCCAGCCT CCTATGCAAG TAGGCACAGC 
TCCTTTAGCC GAAGTTTTGG AGGACGGAGC CAGGCGGCAG CACTTTTAGC TGCCACTGTC 
CCAGAGCAGA GGACTGTGAC CCTGGATGTT GACGTGAACA ACCGCACAGA CCGGCTGGAG 
TGGTGCAGCT GTTATTACCA TGGCAACTTT TCTCTGAATG CAGCCTTTGA GATCAAGCTG 
CACTGGATGG CGGTGACCGC AGCAGTACTC TTCGAGATGG TCCAAGGTTG GCATCGGAAA 
GCCACCTCCT GTGGCTTCTT GTTAGTCCCA GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT 
TACCTGTATG GCGACCCCCT TCGTGCCCAG CTCTTCATCC CACTCAACAT CAGCTGCTTG 
CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT AGCTTTGAAC CCGAAACGTA CTGGGATCGA 
ATGCACCTCT TCCAGGAAGC CATTGCACAC AGGTTTGGGT TTGTACAAGA TAAATATTCT 
GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCACGT TACAGGAACA 
GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG 
AACTCCACCA GCTCCACCAA CCAGAACATG TTCTGCGAGG AGCGGGTCGG CTACAACTGG 
GCCTACAACA CCATGCTCAC CAAAACATGG CGCTCCAGCG CCACAGGGGA TGAAAAGTTT 
GCTGATCGGC TGCTGAAGGA CTTCACGGAC TTCTGCATCA ACCGTGACAA CCGGCTGGTC 
ACGTTCTGGA CAAGTTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356831.1
CDS	90..4817
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001369902.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369902.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG TCAGCACATG TGCCTATATC 
ACCCAGAAGG TGGAGTTTGC TGGCATCAGA GCACAGGCTG GTGAACTGTG GGTTAAGAAT 
GAGAAGGTCA TGTGTGGCTA CATCAGTGAA GATACCAGGG TGGTGTTTCG TTCTACGTCG 
GCTATGGTTT ACATATTTAT TCAGATGAGC TGTGAAATGT GGGATTTTGA TATTTATGGG 
GATTTGTATT TTGAGAAAGC TGTGAATGGT TTCCTTGCTG ATCTATTTAC CAAGTGGAAG 
GAGAAGAACT GTAGTCATGA AGTGACAGTG GTCCTGTTTT CTAGAACTTT CTATGATGCA 
AAATCTGTTG ATGAATTTCC TGAAATAAAC CGAGCCTCAA TTCGACAGGA TCACAAGGGG 
AGATTCTATG AAGACTTTTA CAAAGTGGTG GTGCAGAATG AGAGAAGAGA AGAATGGACT 
TCACTTCTCG TAACCATTAA AAAACTCTTC ATCCAGTATC CAGTGTTGGT GCGACTGGAA 
CAGGCAGAGG GCTTTCCTCA AGGAGATAAT TCTACCTCAG CACAAGGAAA CTACCTGGAG 
GCCATCAATC TGTCATTCAA TGTGTTTGAT AAGCACTACA TCAACCGCAA CTTTGACCGA 
ACTGGGCAGA TGTCAGTGGT GATCACGCCC GGGGTGGGTG TCTTTGAAGT GGACCGCCTA 
CTCATGATCC TGACCAAGCA GCGGATGATA GATAATGGAA TTGGTGTGGA TTTGGTGTGC 
ATGGGAGAGC AACCGTTACA TGCTGTCCCA TTGTTCAAGC TCCATAATCG GAGTGCTCCC 
CGTGATTCTC GTCTGGGCGA TGACTATAAT ATCCCTCACT GGATAAACCA CAGTTTCTAC 
ACATCCAAAA GCCAGCTCTT TTGTAATAGT TTCACCCCAC GAATAAAACT GGCAGGAAAG 
AAGCCCGCCT CTGAGAAAGC AAAAAATGGC CGTGATACAT CTCTCGGGAG TCCAAAAGAA 
TCTGAGAACG CCCTTCCCAT CCAAGTAGAT TATGACGCCT ATGACGCTCA AGTGTTCAGG 
CTGCCCGGCC CATCCCGGGC CCAGTGCCTC ACCACCTGCA GATCTGTGCG AGAGCGAGAG 
AGTCACAGTC GAAAGAGTGC CAGCTCCTGT GATGTTTCAT CCAGCCCTTC CCTACCAAGC 
CGCACACTGC CCACTGAGGA AGTGAGGAGC CAGGCTTCTG ACGACAGCTC CCTAGGCAAG 
AGTGCCAACA TCCTGATGAT CCCACACCCC CACCTGCACC AGTATGAAGT CAGCAGCTCC 
TTGGGATACA CCAGCACTCG AGATGTCCTG GAGAACATGA TGGAGCCACC ACAGCGAGAC 
TCCAGTGCAC CAGGGAGGTT TCACGTTGGC AGTGCAGAAT CCATGCTGCA TGTTCGACCT 
GGTGGATACA CGCCCCAGAG AGCACTGATT AACCCCTTCG CTCCCTCTCG GATGCCCATG 
AAGCTTACGT CCAACAGAAG GCGCTGGATG CACACTTTTC CTGTGGGGCC ATCCGGAGAA 
GCCATCCAGA TCCACCACCA GACCCGACAG AATATGGCGG AGCTACAAGG CAGCGGGCAG 
AGGGATCCAA CTCACTCCTC TGCAGAGCTG CTGGAGTTAG CATATCATGA AGCTGCTGGA 
AGGCACAGCA ATTCCCGCCA GCCTGGTGAC GGCATGTCCT TCTTGAACTT CAGTGGAACA 
GAGGAGCTTT CTGTCGGCCT GCTTAGCAAC AGTGGTGCAG GTATGAATCC TAGGACCCAG 
AATAAGGATT CTCTAGAGGA CAGTGTTTCT ACCTCTCCAG ACCCAATTCT GACACTGTCT 
GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC ACAGTTGGAG TGGACTGGAA GTCTCTCACT 
ACTCCGGCGT GCCTCCCCCT TACCACCGAC TACTTCCCTG ACCGCCAGGG CCTGCAGAAT 
GACTACACAG AGGGCTGTTA TGATCTCCTT CCAGAAGCAG ACATCGACAG GAGGGACGAA 
GATGGTGTGC AGATGACAGC CCAGCAGGTA TTTGAAGAGT TTATTTGCCA ACGTCTCATG 
CAGGGCTACC AAATCATAGT GCAGCCCAAG ACACAGAAAC CCAATCCTGC TGTCCCGCCC 
CCGCTGAGCA GTAGCCCACT CTATAGCCGA GGCCTTGTGT CCCGAAACCG CCCTGAGGAG 
GAGGACCAGT ATTGGCTGAG TATGGGCAGA ACGTTCCACA AAGTGACGCT GAAGGATAAG 
ATGATCACAG TGACGCGATA CCTTCCCAAG TATCCTTATG AATCTGCCCA GATCCACTAC 
ACCTACAGCC TCTGTCCTTC CCACTCAGAC TCAGAGTTCG TCTCCTGCTG GGTGGAATTC 
TCCCACGAAC GGCTGGAGGA GTACAAGTGG AATTACTTAG ATCAGTATAT CTGTTCTGCC 
GGCTCTGAAG ACTTCAGCTT AATTGAGTCC CTGAAGTTCT GGAGGACCCG CTTCCTGCTG 
CTGCCAGCCT GTGTCACCGC CACCAAGCGC ATCACGGAGG GGGAGGCCCA CTGCGACATC 
TATGGGGACA GGCCCCGTGC AGACGAGGAC GAGTGGCAAC TCCTGGATGG TTTTGTCCGC 
TTTGTGGAGG GCTTGAATCG CATTCGCAGG CGGCATCGCT CGGATCGCAT GATGCGGAAA 
GGGACCGCCA TGAAAGGCTT GCAGATGACT GGGCCCATTT CCACGCATTC TCTGGAGTCA 
ACTGCACCCC CAGTGGGGAA GAAGGGAACC TCAGCTCTCT CTGCCCTGTT GGAGATGGAG 
GCCAGTCAGA AGTGCCTGGG AGAACAGCAG GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC 
CAGTCAGCCG AGAGCAGCAG CGTTGCCATG ACTCCCACCT ACATGGACAG CCCACGAAAG 
GACGGGGCCT TCTTTATGGA GTTTGTCCGC AGCCCACGCA CAGCATCGTC CGCCTTCTAC 
CCTCAGGTAT CTGTGGACCA AACAGCCACT CCTATGTTGG ACGGCACCAG TTTGGGCATA 
TGCACAGGCC AATCCATGGA CAGAGGCAAC AGCCAGACCT TTGGGAACTC CCAGAACATA 
GGAGAACAGG GCTACTCCTC CACAAACTCC AGTGACAGCA GCTCTCAGCA GCTGGTGGCA 
AGCTCCTTGA CCTCATCCTC TACCCTGACA GAGATCCTGG AAGCCATGAA GCACCCCTCG 
ACAGGAGTCC AGCTGCTCTC TGAACAGAAG GGCCTCTCAC CGTACTGCTT CATCAGCGCG 
GAGGTGGTAC ACTGGTTGGT GAACCACGTG GAGGGGATCC AGACACAGGC GATGGCCATT 
GACATCATGC AGAAAATGCT GGAAGAGCAG CTCATCACAC ATGCATCTGG CGAAGCCTGG 
CGGACCTTCA TCTACGGCTT CTATTTCTAC AAGATAGTAA CGGACAAAGA GCCCGACCGA 
GTGGCCATGC AGCAGCCCGC CACCACCTGG CACACAGCAG GAGTGGACGA CTTCGCCAGC 
TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCAGAAG AGCTCGTGCA CTCTGAGATT 
CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC CGGCCAGCCT CCTATGCAAG TAGGCACAGC 
TCCTTTAGCC GAAGTTTTGG AGGACGGAGC CAGGCGGCAG CACTTTTAGC TGCCACTGTC 
CCAGAGCAGA GGACTGTGAC CCTGGATGTT GACGTGAACA ACCGCACAGA CCGGCTGGAG 
TGGTGCAGCT GTTATTACCA TGGCAACTTT TCTCTGAATG CAGCCTTTGA GATCAAGCTG 
CACTGGATGG CGGTGACCGC AGCAGTACTC TTCGAGATGG TCCAAGGTTG GCATCGGAAA 
GCCACCTCCT GTGGCTTCTT GTTAGTCCCA GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT 
TACCTGTATG GCGACCCCCT TCGTGCCCAG CTCTTCATCC CACTCAACAT CAGCTGCTTG 
CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT AGCTTTGAAC CCGAAACGTA CTGGGATCGA 
ATGCACCTCT TCCAGGAAGC CATTGCACAC AGGTTTGGGT TTGTACAAGA TAAATATTCT 
GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCACGT TACAGGAACA 
GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG 
AACTCCACCA GCTCCACCAA CCAGAACATG TTCTGCGAGG AGCGGGTCGG CTACAACTGG 
GCCTACAACA CCATGCTCAC CAAAACATGG CGCTCCAGCG CCACAGGGGA TGAAAAGTTT 
GCTGATCGGC TGCTGAAGGA CTTCACGGAC TTCTGCATCA ACCGTGACAA CCGGCTGGTC 
ACGTTCTGGA CAAGTTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001369901.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356830.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu108683
Clone ID Related Accession (Same CDS sequence)	NM_001369902.1 , NM_001369901.1
Accession Version	NM_001369901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.134695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG TCAGCACATG TGCCTATATC 
ACCCAGAAGG TGGAGTTTGC TGGCATCAGA GCACAGGCTG GTGAACTGTG GGTTAAGAAT 
GAGAAGGTCA TGTGTGGCTA CATCAGTGAA GATACCAGGG TGGTGTTTCG TTCTACGTCG 
GCTATGGTTT ACATATTTAT TCAGATGAGC TGTGAAATGT GGGATTTTGA TATTTATGGG 
GATTTGTATT TTGAGAAAGC TGTGAATGGT TTCCTTGCTG ATCTATTTAC CAAGTGGAAG 
GAGAAGAACT GTAGTCATGA AGTGACAGTG GTCCTGTTTT CTAGAACTTT CTATGATGCA 
AAATCTGTTG ATGAATTTCC TGAAATAAAC CGAGCCTCAA TTCGACAGGA TCACAAGGGG 
AGATTCTATG AAGACTTTTA CAAAGTGGTG GTGCAGAATG AGAGAAGAGA AGAATGGACT 
TCACTTCTCG TAACCATTAA AAAACTCTTC ATCCAGTATC CAGTGTTGGT GCGACTGGAA 
CAGGCAGAGG GCTTTCCTCA AGGAGATAAT TCTACCTCAG CACAAGGAAA CTACCTGGAG 
GCCATCAATC TGTCATTCAA TGTGTTTGAT AAGCACTACA TCAACCGCAA CTTTGACCGA 
ACTGGGCAGA TGTCAGTGGT GATCACGCCC GGGGTGGGTG TCTTTGAAGT GGACCGCCTA 
CTCATGATCC TGACCAAGCA GCGGATGATA GATAATGGAA TTGGTGTGGA TTTGGTGTGC 
ATGGGAGAGC AACCGTTACA TGCTGTCCCA TTGTTCAAGC TCCATAATCG GAGTGCTCCC 
CGTGATTCTC GTCTGGGCGA TGACTATAAT ATCCCTCACT GGATAAACCA CAGTTTCTAC 
ACATCCAAAA GCCAGCTCTT TTGTAATAGT TTCACCCCAC GAATAAAACT GGCAGGAAAG 
AAGCCCGCCT CTGAGAAAGC AAAAAATGGC CGTGATACAT CTCTCGGGAG TCCAAAAGAA 
TCTGAGAACG CCCTTCCCAT CCAAGTAGAT TATGACGCCT ATGACGCTCA AGTGTTCAGG 
CTGCCCGGCC CATCCCGGGC CCAGTGCCTC ACCACCTGCA GATCTGTGCG AGAGCGAGAG 
AGTCACAGTC GAAAGAGTGC CAGCTCCTGT GATGTTTCAT CCAGCCCTTC CCTACCAAGC 
CGCACACTGC CCACTGAGGA AGTGAGGAGC CAGGCTTCTG ACGACAGCTC CCTAGGCAAG 
AGTGCCAACA TCCTGATGAT CCCACACCCC CACCTGCACC AGTATGAAGT CAGCAGCTCC 
TTGGGATACA CCAGCACTCG AGATGTCCTG GAGAACATGA TGGAGCCACC ACAGCGAGAC 
TCCAGTGCAC CAGGGAGGTT TCACGTTGGC AGTGCAGAAT CCATGCTGCA TGTTCGACCT 
GGTGGATACA CGCCCCAGAG AGCACTGATT AACCCCTTCG CTCCCTCTCG GATGCCCATG 
AAGCTTACGT CCAACAGAAG GCGCTGGATG CACACTTTTC CTGTGGGGCC ATCCGGAGAA 
GCCATCCAGA TCCACCACCA GACCCGACAG AATATGGCGG AGCTACAAGG CAGCGGGCAG 
AGGGATCCAA CTCACTCCTC TGCAGAGCTG CTGGAGTTAG CATATCATGA AGCTGCTGGA 
AGGCACAGCA ATTCCCGCCA GCCTGGTGAC GGCATGTCCT TCTTGAACTT CAGTGGAACA 
GAGGAGCTTT CTGTCGGCCT GCTTAGCAAC AGTGGTGCAG GTATGAATCC TAGGACCCAG 
AATAAGGATT CTCTAGAGGA CAGTGTTTCT ACCTCTCCAG ACCCAATTCT GACACTGTCT 
GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC ACAGTTGGAG TGGACTGGAA GTCTCTCACT 
ACTCCGGCGT GCCTCCCCCT TACCACCGAC TACTTCCCTG ACCGCCAGGG CCTGCAGAAT 
GACTACACAG AGGGCTGTTA TGATCTCCTT CCAGAAGCAG ACATCGACAG GAGGGACGAA 
GATGGTGTGC AGATGACAGC CCAGCAGGTA TTTGAAGAGT TTATTTGCCA ACGTCTCATG 
CAGGGCTACC AAATCATAGT GCAGCCCAAG ACACAGAAAC CCAATCCTGC TGTCCCGCCC 
CCGCTGAGCA GTAGCCCACT CTATAGCCGA GGCCTTGTGT CCCGAAACCG CCCTGAGGAG 
GAGGACCAGT ATTGGCTGAG TATGGGCAGA ACGTTCCACA AAGTGACGCT GAAGGATAAG 
ATGATCACAG TGACGCGATA CCTTCCCAAG TATCCTTATG AATCTGCCCA GATCCACTAC 
ACCTACAGCC TCTGTCCTTC CCACTCAGAC TCAGAGTTCG TCTCCTGCTG GGTGGAATTC 
TCCCACGAAC GGCTGGAGGA GTACAAGTGG AATTACTTAG ATCAGTATAT CTGTTCTGCC 
GGCTCTGAAG ACTTCAGCTT AATTGAGTCC CTGAAGTTCT GGAGGACCCG CTTCCTGCTG 
CTGCCAGCCT GTGTCACCGC CACCAAGCGC ATCACGGAGG GGGAGGCCCA CTGCGACATC 
TATGGGGACA GGCCCCGTGC AGACGAGGAC GAGTGGCAAC TCCTGGATGG TTTTGTCCGC 
TTTGTGGAGG GCTTGAATCG CATTCGCAGG CGGCATCGCT CGGATCGCAT GATGCGGAAA 
GGGACCGCCA TGAAAGGCTT GCAGATGACT GGGCCCATTT CCACGCATTC TCTGGAGTCA 
ACTGCACCCC CAGTGGGGAA GAAGGGAACC TCAGCTCTCT CTGCCCTGTT GGAGATGGAG 
GCCAGTCAGA AGTGCCTGGG AGAACAGCAG GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC 
CAGTCAGCCG AGAGCAGCAG CGTTGCCATG ACTCCCACCT ACATGGACAG CCCACGAAAG 
GACGGGGCCT TCTTTATGGA GTTTGTCCGC AGCCCACGCA CAGCATCGTC CGCCTTCTAC 
CCTCAGGTAT CTGTGGACCA AACAGCCACT CCTATGTTGG ACGGCACCAG TTTGGGCATA 
TGCACAGGCC AATCCATGGA CAGAGGCAAC AGCCAGACCT TTGGGAACTC CCAGAACATA 
GGAGAACAGG GCTACTCCTC CACAAACTCC AGTGACAGCA GCTCTCAGCA GCTGGTGGCA 
AGCTCCTTGA CCTCATCCTC TACCCTGACA GAGATCCTGG AAGCCATGAA GCACCCCTCG 
ACAGGAGTCC AGCTGCTCTC TGAACAGAAG GGCCTCTCAC CGTACTGCTT CATCAGCGCG 
GAGGTGGTAC ACTGGTTGGT GAACCACGTG GAGGGGATCC AGACACAGGC GATGGCCATT 
GACATCATGC AGAAAATGCT GGAAGAGCAG CTCATCACAC ATGCATCTGG CGAAGCCTGG 
CGGACCTTCA TCTACGGCTT CTATTTCTAC AAGATAGTAA CGGACAAAGA GCCCGACCGA 
GTGGCCATGC AGCAGCCCGC CACCACCTGG CACACAGCAG GAGTGGACGA CTTCGCCAGC 
TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCAGAAG AGCTCGTGCA CTCTGAGATT 
CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC CGGCCAGCCT CCTATGCAAG TAGGCACAGC 
TCCTTTAGCC GAAGTTTTGG AGGACGGAGC CAGGCGGCAG CACTTTTAGC TGCCACTGTC 
CCAGAGCAGA GGACTGTGAC CCTGGATGTT GACGTGAACA ACCGCACAGA CCGGCTGGAG 
TGGTGCAGCT GTTATTACCA TGGCAACTTT TCTCTGAATG CAGCCTTTGA GATCAAGCTG 
CACTGGATGG CGGTGACCGC AGCAGTACTC TTCGAGATGG TCCAAGGTTG GCATCGGAAA 
GCCACCTCCT GTGGCTTCTT GTTAGTCCCA GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT 
TACCTGTATG GCGACCCCCT TCGTGCCCAG CTCTTCATCC CACTCAACAT CAGCTGCTTG 
CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT AGCTTTGAAC CCGAAACGTA CTGGGATCGA 
ATGCACCTCT TCCAGGAAGC CATTGCACAC AGGTTTGGGT TTGTACAAGA TAAATATTCT 
GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCACGT TACAGGAACA 
GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG 
AACTCCACCA GCTCCACCAA CCAGAACATG TTCTGCGAGG AGCGGGTCGG CTACAACTGG 
GCCTACAACA CCATGCTCAC CAAAACATGG CGCTCCAGCG CCACAGGGGA TGAAAAGTTT 
GCTGATCGGC TGCTGAAGGA CTTCACGGAC TTCTGCATCA ACCGTGACAA CCGGCTGGTC 
ACGTTCTGGA CAAGTTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356830.1
CDS	203..4930
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001369901.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369901.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG TCAGCACATG TGCCTATATC 
ACCCAGAAGG TGGAGTTTGC TGGCATCAGA GCACAGGCTG GTGAACTGTG GGTTAAGAAT 
GAGAAGGTCA TGTGTGGCTA CATCAGTGAA GATACCAGGG TGGTGTTTCG TTCTACGTCG 
GCTATGGTTT ACATATTTAT TCAGATGAGC TGTGAAATGT GGGATTTTGA TATTTATGGG 
GATTTGTATT TTGAGAAAGC TGTGAATGGT TTCCTTGCTG ATCTATTTAC CAAGTGGAAG 
GAGAAGAACT GTAGTCATGA AGTGACAGTG GTCCTGTTTT CTAGAACTTT CTATGATGCA 
AAATCTGTTG ATGAATTTCC TGAAATAAAC CGAGCCTCAA TTCGACAGGA TCACAAGGGG 
AGATTCTATG AAGACTTTTA CAAAGTGGTG GTGCAGAATG AGAGAAGAGA AGAATGGACT 
TCACTTCTCG TAACCATTAA AAAACTCTTC ATCCAGTATC CAGTGTTGGT GCGACTGGAA 
CAGGCAGAGG GCTTTCCTCA AGGAGATAAT TCTACCTCAG CACAAGGAAA CTACCTGGAG 
GCCATCAATC TGTCATTCAA TGTGTTTGAT AAGCACTACA TCAACCGCAA CTTTGACCGA 
ACTGGGCAGA TGTCAGTGGT GATCACGCCC GGGGTGGGTG TCTTTGAAGT GGACCGCCTA 
CTCATGATCC TGACCAAGCA GCGGATGATA GATAATGGAA TTGGTGTGGA TTTGGTGTGC 
ATGGGAGAGC AACCGTTACA TGCTGTCCCA TTGTTCAAGC TCCATAATCG GAGTGCTCCC 
CGTGATTCTC GTCTGGGCGA TGACTATAAT ATCCCTCACT GGATAAACCA CAGTTTCTAC 
ACATCCAAAA GCCAGCTCTT TTGTAATAGT TTCACCCCAC GAATAAAACT GGCAGGAAAG 
AAGCCCGCCT CTGAGAAAGC AAAAAATGGC CGTGATACAT CTCTCGGGAG TCCAAAAGAA 
TCTGAGAACG CCCTTCCCAT CCAAGTAGAT TATGACGCCT ATGACGCTCA AGTGTTCAGG 
CTGCCCGGCC CATCCCGGGC CCAGTGCCTC ACCACCTGCA GATCTGTGCG AGAGCGAGAG 
AGTCACAGTC GAAAGAGTGC CAGCTCCTGT GATGTTTCAT CCAGCCCTTC CCTACCAAGC 
CGCACACTGC CCACTGAGGA AGTGAGGAGC CAGGCTTCTG ACGACAGCTC CCTAGGCAAG 
AGTGCCAACA TCCTGATGAT CCCACACCCC CACCTGCACC AGTATGAAGT CAGCAGCTCC 
TTGGGATACA CCAGCACTCG AGATGTCCTG GAGAACATGA TGGAGCCACC ACAGCGAGAC 
TCCAGTGCAC CAGGGAGGTT TCACGTTGGC AGTGCAGAAT CCATGCTGCA TGTTCGACCT 
GGTGGATACA CGCCCCAGAG AGCACTGATT AACCCCTTCG CTCCCTCTCG GATGCCCATG 
AAGCTTACGT CCAACAGAAG GCGCTGGATG CACACTTTTC CTGTGGGGCC ATCCGGAGAA 
GCCATCCAGA TCCACCACCA GACCCGACAG AATATGGCGG AGCTACAAGG CAGCGGGCAG 
AGGGATCCAA CTCACTCCTC TGCAGAGCTG CTGGAGTTAG CATATCATGA AGCTGCTGGA 
AGGCACAGCA ATTCCCGCCA GCCTGGTGAC GGCATGTCCT TCTTGAACTT CAGTGGAACA 
GAGGAGCTTT CTGTCGGCCT GCTTAGCAAC AGTGGTGCAG GTATGAATCC TAGGACCCAG 
AATAAGGATT CTCTAGAGGA CAGTGTTTCT ACCTCTCCAG ACCCAATTCT GACACTGTCT 
GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC ACAGTTGGAG TGGACTGGAA GTCTCTCACT 
ACTCCGGCGT GCCTCCCCCT TACCACCGAC TACTTCCCTG ACCGCCAGGG CCTGCAGAAT 
GACTACACAG AGGGCTGTTA TGATCTCCTT CCAGAAGCAG ACATCGACAG GAGGGACGAA 
GATGGTGTGC AGATGACAGC CCAGCAGGTA TTTGAAGAGT TTATTTGCCA ACGTCTCATG 
CAGGGCTACC AAATCATAGT GCAGCCCAAG ACACAGAAAC CCAATCCTGC TGTCCCGCCC 
CCGCTGAGCA GTAGCCCACT CTATAGCCGA GGCCTTGTGT CCCGAAACCG CCCTGAGGAG 
GAGGACCAGT ATTGGCTGAG TATGGGCAGA ACGTTCCACA AAGTGACGCT GAAGGATAAG 
ATGATCACAG TGACGCGATA CCTTCCCAAG TATCCTTATG AATCTGCCCA GATCCACTAC 
ACCTACAGCC TCTGTCCTTC CCACTCAGAC TCAGAGTTCG TCTCCTGCTG GGTGGAATTC 
TCCCACGAAC GGCTGGAGGA GTACAAGTGG AATTACTTAG ATCAGTATAT CTGTTCTGCC 
GGCTCTGAAG ACTTCAGCTT AATTGAGTCC CTGAAGTTCT GGAGGACCCG CTTCCTGCTG 
CTGCCAGCCT GTGTCACCGC CACCAAGCGC ATCACGGAGG GGGAGGCCCA CTGCGACATC 
TATGGGGACA GGCCCCGTGC AGACGAGGAC GAGTGGCAAC TCCTGGATGG TTTTGTCCGC 
TTTGTGGAGG GCTTGAATCG CATTCGCAGG CGGCATCGCT CGGATCGCAT GATGCGGAAA 
GGGACCGCCA TGAAAGGCTT GCAGATGACT GGGCCCATTT CCACGCATTC TCTGGAGTCA 
ACTGCACCCC CAGTGGGGAA GAAGGGAACC TCAGCTCTCT CTGCCCTGTT GGAGATGGAG 
GCCAGTCAGA AGTGCCTGGG AGAACAGCAG GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC 
CAGTCAGCCG AGAGCAGCAG CGTTGCCATG ACTCCCACCT ACATGGACAG CCCACGAAAG 
GACGGGGCCT TCTTTATGGA GTTTGTCCGC AGCCCACGCA CAGCATCGTC CGCCTTCTAC 
CCTCAGGTAT CTGTGGACCA AACAGCCACT CCTATGTTGG ACGGCACCAG TTTGGGCATA 
TGCACAGGCC AATCCATGGA CAGAGGCAAC AGCCAGACCT TTGGGAACTC CCAGAACATA 
GGAGAACAGG GCTACTCCTC CACAAACTCC AGTGACAGCA GCTCTCAGCA GCTGGTGGCA 
AGCTCCTTGA CCTCATCCTC TACCCTGACA GAGATCCTGG AAGCCATGAA GCACCCCTCG 
ACAGGAGTCC AGCTGCTCTC TGAACAGAAG GGCCTCTCAC CGTACTGCTT CATCAGCGCG 
GAGGTGGTAC ACTGGTTGGT GAACCACGTG GAGGGGATCC AGACACAGGC GATGGCCATT 
GACATCATGC AGAAAATGCT GGAAGAGCAG CTCATCACAC ATGCATCTGG CGAAGCCTGG 
CGGACCTTCA TCTACGGCTT CTATTTCTAC AAGATAGTAA CGGACAAAGA GCCCGACCGA 
GTGGCCATGC AGCAGCCCGC CACCACCTGG CACACAGCAG GAGTGGACGA CTTCGCCAGC 
TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCAGAAG AGCTCGTGCA CTCTGAGATT 
CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC CGGCCAGCCT CCTATGCAAG TAGGCACAGC 
TCCTTTAGCC GAAGTTTTGG AGGACGGAGC CAGGCGGCAG CACTTTTAGC TGCCACTGTC 
CCAGAGCAGA GGACTGTGAC CCTGGATGTT GACGTGAACA ACCGCACAGA CCGGCTGGAG 
TGGTGCAGCT GTTATTACCA TGGCAACTTT TCTCTGAATG CAGCCTTTGA GATCAAGCTG 
CACTGGATGG CGGTGACCGC AGCAGTACTC TTCGAGATGG TCCAAGGTTG GCATCGGAAA 
GCCACCTCCT GTGGCTTCTT GTTAGTCCCA GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT 
TACCTGTATG GCGACCCCCT TCGTGCCCAG CTCTTCATCC CACTCAACAT CAGCTGCTTG 
CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT AGCTTTGAAC CCGAAACGTA CTGGGATCGA 
ATGCACCTCT TCCAGGAAGC CATTGCACAC AGGTTTGGGT TTGTACAAGA TAAATATTCT 
GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCACGT TACAGGAACA 
GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG 
AACTCCACCA GCTCCACCAA CCAGAACATG TTCTGCGAGG AGCGGGTCGG CTACAACTGG 
GCCTACAACA CCATGCTCAC CAAAACATGG CGCTCCAGCG CCACAGGGGA TGAAAAGTTT 
GCTGATCGGC TGCTGAAGGA CTTCACGGAC TTCTGCATCA ACCGTGACAA CCGGCTGGTC 
ACGTTCTGGA CAAGTTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001242897.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229826.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 5, mRNA.

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CloneID	OHu19567
Clone ID Related Accession (Same CDS sequence)	NM_001242897.1 , NM_001242897.2 , XM_011530563.2
Accession Version	NM_001242897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA339804.1, AK294987.1 and DB381506.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (5) differs in the 5' UTR and lacks three in-frame exons in the coding region compared to variant 4. The encoded protein (isoform 5) is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.131363.1, SRR1803616.140338.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229826.1
CDS	107..4618
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001242897.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242897.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001242897.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229826.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 5, mRNA.

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CloneID	OHu19567
Clone ID Related Accession (Same CDS sequence)	NM_001242897.1 , NM_001242897.2 , XM_011530563.2
Accession Version	NM_001242897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. This sequence is a reference standard in the RefSeqGene project. On Apr 24, 2019 this sequence version replaced NM_001242897.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.104870.1, SRR1803611.180135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229826.1
CDS	90..4601
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001242897.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242897.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001136029.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129501.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 3, mRNA.

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CloneID	OHu22513
Clone ID Related Accession (Same CDS sequence)	NM_001136029.2 , XM_017029109.1 , NM_001136029.3 , XM_011530557.2
Accession Version	NM_001136029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AJ698950.1 and DB381506.1. On Jul 2, 2011 this sequence version replaced NM_001136029.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in an internal exon compared to variant 4. The encoded protein (isoform 3) is shorter than isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ698950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129501.1
CDS	61..4845
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001136029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136029.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001136029.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129501.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 3, mRNA.

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CloneID	OHu22513
Clone ID Related Accession (Same CDS sequence)	NM_001136029.2 , XM_017029109.1 , NM_001136029.3 , XM_011530557.2
Accession Version	NM_001136029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AJ698950.1 and DB381506.1. On or before Jun 16, 2018 this sequence version replaced XM_017029109.1, NM_001136029.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.258848.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129501.1
CDS	90..4874
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001136029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136029.3

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_014662.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055477.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23642
Clone ID Related Accession (Same CDS sequence)	XM_011530559.2 , NM_014662.4 , NM_014662.5 , NM_001369903.1 , XM_017029111.1
Accession Version	NM_014662.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, BC146766.1 and DB381506.1. On Mar 22, 2014 this sequence version replaced NM_014662.3. Transcript Variant: This variant (1) uses an alternate in-frame splice site in an internal exon and lacks an in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 1) is shorter than isoform 4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146766.1, AB014545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055477.1
CDS	61..4779
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_014662.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014662.4

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_014662.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055477.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 1, mRNA.

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CloneID	OHu23642
Clone ID Related Accession (Same CDS sequence)	XM_011530559.2 , NM_014662.4 , NM_014662.5 , NM_001369903.1 , XM_017029111.1
Accession Version	NM_014662.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, BC146766.1 and DB381506.1. On or before Jun 16, 2018 this sequence version replaced XM_017029111.1, NM_014662.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.139866.1, SRR1803612.168344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055477.1
CDS	90..4808
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_014662.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014662.5

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001369903.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356832.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 18, mRNA.

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CloneID	OHu23642
Clone ID Related Accession (Same CDS sequence)	XM_011530559.2 , NM_014662.4 , NM_014662.5 , NM_001369903.1 , XM_017029111.1
Accession Version	NM_001369903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Apr 24, 2019 this sequence version replaced XM_011530559.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC146766.1, AB014545.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356832.1
CDS	203..4921
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001369903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369903.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001363852.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350781.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 8, mRNA.

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CloneID	OHu53768
Clone ID Related Accession (Same CDS sequence)	NM_001363852.1 , NM_001363852.2 , XM_011530558.2 , NM_001364320.1 , XM_017029110.1
Accession Version	NM_001363852.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Jun 3, 2018 this sequence version replaced XM_017029110.1. Transcript Variant: This variant (8) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 4. The resulting isoform (6) has the same N- and C-termini but is shorter compared to isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.182786.1, SRR1660809.13835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350781.1
CDS	107..4852
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001363852.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363852.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001363852.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350781.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 8, mRNA.

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CloneID	OHu53768
Clone ID Related Accession (Same CDS sequence)	NM_001363852.1 , NM_001363852.2 , XM_011530558.2 , NM_001364320.1 , XM_017029110.1
Accession Version	NM_001363852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Apr 24, 2019 this sequence version replaced NM_001363852.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.25318.1, SRR1803616.182786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350781.1
CDS	90..4835
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001363852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363852.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001364320.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351249.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 15, mRNA.

Price & Availability↑

CloneID	OHu53768
Clone ID Related Accession (Same CDS sequence)	NM_001363852.1 , NM_001363852.2 , XM_011530558.2 , NM_001364320.1 , XM_017029110.1
Accession Version	NM_001364320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Jun 16, 2018 this sequence version replaced XM_011530558.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.252795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351249.1
CDS	207..4952
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001364320.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364320.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001363854.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350783.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 9, mRNA.

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CloneID	OHu53769
Clone ID Related Accession (Same CDS sequence)	NM_001363854.1 , NM_001364319.1 , XM_017029112.1 , XM_011530560.2 , NM_001363854.2
Accession Version	NM_001363854.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Jun 3, 2018 this sequence version replaced XM_017029112.1. Transcript Variant: This variant (9) differs in the 5' UTR and lacks two alternate exons compared to variant 4. The resulting isoform (7) has the same N- and C-termini but is shorter compared to isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.19432.1, SRR1803612.3037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350783.1
CDS	107..4684
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001363854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363854.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001364319.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351248.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 13, mRNA.

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CloneID	OHu53769
Clone ID Related Accession (Same CDS sequence)	NM_001363854.1 , NM_001364319.1 , XM_017029112.1 , XM_011530560.2 , NM_001363854.2
Accession Version	NM_001364319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Jun 16, 2018 this sequence version replaced XM_011530560.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.92225.1, SRR1660809.68163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351248.1
CDS	207..4784
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001364319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364319.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( NM_001363854.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350783.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 9, mRNA.

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CloneID	OHu53769
Clone ID Related Accession (Same CDS sequence)	NM_001363854.1 , NM_001364319.1 , XM_017029112.1 , XM_011530560.2 , NM_001363854.2
Accession Version	NM_001363854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-30
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005004.3, AL022331.1, Z82190.1 and Z83856.2. On Apr 24, 2019 this sequence version replaced NM_001363854.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.160979.1, SRR1803612.3037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350783.1
CDS	90..4667
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	NM_001363854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363854.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530563.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528865.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X12, mRNA.

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CloneID	OHu19567
Clone ID Related Accession (Same CDS sequence)	NM_001242897.1 , NM_001242897.2 , XM_011530563.2
Accession Version	XM_011530563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528865.1
CDS	210..4721
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530563.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530563.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGTCCAAG GTTGGCATCG GAAAGCCACC TCCTGTGGCT TCTTGTTAGT CCCAGTTTTG 
GAGGGGCCTT TTGCACTGCC CAGTTACCTG TATGGCGACC CCCTTCGTGC CCAGCTCTTC 
ATCCCACTCA ACATCAGCTG CTTGCTCAAG GAGGGCAGCG AGCACCTGTT TGATAGCTTT 
GAACCCGAAA CGTACTGGGA TCGAATGCAC CTCTTCCAGG AAGCCATTGC ACACAGGTTT 
GGGTTTGTAC AAGATAAATA TTCTGCCTCT GCTTTTAACT TCCCTGCTGA GAACAAGCCT 
CAGTATATCC ACGTTACAGG AACAGTGTTT CTGCAGCTGC CCTACTCCAA GCGCAAGTTC 
TCAGGGCAGC AGCGGCGGCG GCGGAACTCC ACCAGCTCCA CCAACCAGAA CATGTTCTGC 
GAGGAGCGGG TCGGCTACAA CTGGGCCTAC AACACCATGC TCACCAAAAC ATGGCGCTCC 
AGCGCCACAG GGGATGAAAA GTTTGCTGAT CGGCTGCTGA AGGACTTCAC GGACTTCTGC 
ATCAACCGTG ACAACCGGCT GGTCACGTTC TGGACAAGTT GCCTGGAGAA GATGCATGCC 
AGTGCCCCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029109.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884598.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu22513
Clone ID Related Accession (Same CDS sequence)	NM_001136029.2 , XM_017029109.1 , NM_001136029.3 , XM_011530557.2
Accession Version	XM_017029109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884598.1
CDS	107..4891
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_017029109.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029109.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530557.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528859.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X2, mRNA.

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CloneID	OHu22513
Clone ID Related Accession (Same CDS sequence)	NM_001136029.2 , XM_017029109.1 , NM_001136029.3 , XM_011530557.2
Accession Version	XM_011530557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528859.1
CDS	210..4994
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530557.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530557.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGAC 
GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCACGCACAG CATCGTCCGC CTTCTACCCT 
CAGGTATCTG TGGACCAAAC AGCCACTCCT ATGTTGGACG GCACCAGTTT GGGCATATGC 
ACAGGCCAAT CCATGGACAG AGGCAACAGC CAGACCTTTG GGAACTCCCA GAACATAGGA 
GAACAGGGCT ACTCCTCCAC AAACTCCAGT GACAGCAGCT CTCAGCAGCT GGTGGCAAGC 
TCCTTGACCT CATCCTCTAC CCTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA 
GGAGTCCAGC TGCTCTCTGA ACAGAAGGGC CTCTCACCGT ACTGCTTCAT CAGCGCGGAG 
GTGGTACACT GGTTGGTGAA CCACGTGGAG GGGATCCAGA CACAGGCGAT GGCCATTGAC 
ATCATGCAGA AAATGCTGGA AGAGCAGCTC ATCACACATG CATCTGGCGA AGCCTGGCGG 
ACCTTCATCT ACGGCTTCTA TTTCTACAAG ATAGTAACGG ACAAAGAGCC CGACCGAGTG 
GCCATGCAGC AGCCCGCCAC CACCTGGCAC ACAGCAGGAG TGGACGACTT CGCCAGCTTC 
CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG GCAGAAGAGC TCGTGCACTC TGAGATTCCT 
GCCTTTCTCC TGCCCTGGCT GCCTAGCCGG CCAGCCTCCT ATGCAAGTAG GCACAGCTCC 
TTTAGCCGAA GTTTTGGAGG ACGGAGCCAG GCGGCAGCAC TTTTAGCTGC CACTGTCCCA 
GAGCAGAGGA CTGTGACCCT GGATGTTGAC GTGAACAACC GCACAGACCG GCTGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTTTCT CTGAATGCAG CCTTTGAGAT CAAGCTGCAC 
TGGATGGCGG TGACCGCAGC AGTACTCTTC GAGATGGTCC AAGGTTGGCA TCGGAAAGCC 
ACCTCCTGTG GCTTCTTGTT AGTCCCAGTT TTGGAGGGGC CTTTTGCACT GCCCAGTTAC 
CTGTATGGCG ACCCCCTTCG TGCCCAGCTC TTCATCCCAC TCAACATCAG CTGCTTGCTC 
AAGGAGGGCA GCGAGCACCT GTTTGATAGC TTTGAACCCG AAACGTACTG GGATCGAATG 
CACCTCTTCC AGGAAGCCAT TGCACACAGG TTTGGGTTTG TACAAGATAA ATATTCTGCC 
TCTGCTTTTA ACTTCCCTGC TGAGAACAAG CCTCAGTATA TCCACGTTAC AGGAACAGTG 
TTTCTGCAGC TGCCCTACTC CAAGCGCAAG TTCTCAGGGC AGCAGCGGCG GCGGCGGAAC 
TCCACCAGCT CCACCAACCA GAACATGTTC TGCGAGGAGC GGGTCGGCTA CAACTGGGCC 
TACAACACCA TGCTCACCAA AACATGGCGC TCCAGCGCCA CAGGGGATGA AAAGTTTGCT 
GATCGGCTGC TGAAGGACTT CACGGACTTC TGCATCAACC GTGACAACCG GCTGGTCACG 
TTCTGGACAA GTTGCCTGGA GAAGATGCAT GCCAGTGCCC CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530559.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528861.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA.

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CloneID	OHu23642
Clone ID Related Accession (Same CDS sequence)	XM_011530559.2 , NM_014662.4 , NM_014662.5 , NM_001369903.1 , XM_017029111.1
Accession Version	XM_011530559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528861.1
CDS	210..4928
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530559.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029111.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884600.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA.

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CloneID	OHu23642
Clone ID Related Accession (Same CDS sequence)	XM_011530559.2 , NM_014662.4 , NM_014662.5 , NM_001369903.1 , XM_017029111.1
Accession Version	XM_017029111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884600.1
CDS	107..4825
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_017029111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029111.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT 
CCGGCGTGCC TCCCCCTTAC CACCGACTAC TTCCCTGACC GCCAGGGCCT GCAGAATGAC 
TACACAGAGG GCTGTTATGA TCTCCTTCCA GAAGCAGACA TCGACAGGAG GGACGAAGAT 
GGTGTGCAGA TGACAGCCCA GCAGGTATTT GAAGAGTTTA TTTGCCAACG TCTCATGCAG 
GGCTACCAAA TCATAGTGCA GCCCAAGACA CAGAAACCCA ATCCTGCTGT CCCGCCCCCG 
CTGAGCAGTA GCCCACTCTA TAGCCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG 
GACCAGTATT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA GGATAAGATG 
ATCACAGTGA CGCGATACCT TCCCAAGTAT CCTTATGAAT CTGCCCAGAT CCACTACACC 
TACAGCCTCT GTCCTTCCCA CTCAGACTCA GAGTTCGTCT CCTGCTGGGT GGAATTCTCC 
CACGAACGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCCGGC 
TCTGAAGACT TCAGCTTAAT TGAGTCCCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG 
CCAGCCTGTG TCACCGCCAC CAAGCGCATC ACGGAGGGGG AGGCCCACTG CGACATCTAT 
GGGGACAGGC CCCGTGCAGA CGAGGACGAG TGGCAACTCC TGGATGGTTT TGTCCGCTTT 
GTGGAGGGCT TGAATCGCAT TCGCAGGCGG CATCGCTCGG ATCGCATGAT GCGGAAAGGG 
ACCGCCATGA AAGGCTTGCA GATGACTGGG CCCATTTCCA CGCATTCTCT GGAGTCAACT 
GCACCCCCAG TGGGGAAGAA GGGAACCTCA GCTCTCTCTG CCCTGTTGGA GATGGAGGCC 
AGTCAGAAGT GCCTGGGAGA ACAGCAGGCA GCTGTGCATG GTGGGAAGAG CTCCGCCCAG 
TCAGCCGAGA GCAGCAGCGT TGCCATGACT CCCACCTACA TGGACAGCCC ACGAAAGGTA 
TCTGTGGACC AAACAGCCAC TCCTATGTTG GACGGCACCA GTTTGGGCAT ATGCACAGGC 
CAATCCATGG ACAGAGGCAA CAGCCAGACC TTTGGGAACT CCCAGAACAT AGGAGAACAG 
GGCTACTCCT CCACAAACTC CAGTGACAGC AGCTCTCAGC AGCTGGTGGC AAGCTCCTTG 
ACCTCATCCT CTACCCTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGAGTC 
CAGCTGCTCT CTGAACAGAA GGGCCTCTCA CCGTACTGCT TCATCAGCGC GGAGGTGGTA 
CACTGGTTGG TGAACCACGT GGAGGGGATC CAGACACAGG CGATGGCCAT TGACATCATG 
CAGAAAATGC TGGAAGAGCA GCTCATCACA CATGCATCTG GCGAAGCCTG GCGGACCTTC 
ATCTACGGCT TCTATTTCTA CAAGATAGTA ACGGACAAAG AGCCCGACCG AGTGGCCATG 
CAGCAGCCCG CCACCACCTG GCACACAGCA GGAGTGGACG ACTTCGCCAG CTTCCAGCGC 
AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTCGTGC ACTCTGAGAT TCCTGCCTTT 
CTCCTGCCCT GGCTGCCTAG CCGGCCAGCC TCCTATGCAA GTAGGCACAG CTCCTTTAGC 
CGAAGTTTTG GAGGACGGAG CCAGGCGGCA GCACTTTTAG CTGCCACTGT CCCAGAGCAG 
AGGACTGTGA CCCTGGATGT TGACGTGAAC AACCGCACAG ACCGGCTGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT TTCTCTGAAT GCAGCCTTTG AGATCAAGCT GCACTGGATG 
GCGGTGACCG CAGCAGTACT CTTCGAGATG GTCCAAGGTT GGCATCGGAA AGCCACCTCC 
TGTGGCTTCT TGTTAGTCCC AGTTTTGGAG GGGCCTTTTG CACTGCCCAG TTACCTGTAT 
GGCGACCCCC TTCGTGCCCA GCTCTTCATC CCACTCAACA TCAGCTGCTT GCTCAAGGAG 
GGCAGCGAGC ACCTGTTTGA TAGCTTTGAA CCCGAAACGT ACTGGGATCG AATGCACCTC 
TTCCAGGAAG CCATTGCACA CAGGTTTGGG TTTGTACAAG ATAAATATTC TGCCTCTGCT 
TTTAACTTCC CTGCTGAGAA CAAGCCTCAG TATATCCACG TTACAGGAAC AGTGTTTCTG 
CAGCTGCCCT ACTCCAAGCG CAAGTTCTCA GGGCAGCAGC GGCGGCGGCG GAACTCCACC 
AGCTCCACCA ACCAGAACAT GTTCTGCGAG GAGCGGGTCG GCTACAACTG GGCCTACAAC 
ACCATGCTCA CCAAAACATG GCGCTCCAGC GCCACAGGGG ATGAAAAGTT TGCTGATCGG 
CTGCTGAAGG ACTTCACGGA CTTCTGCATC AACCGTGACA ACCGGCTGGT CACGTTCTGG 
ACAAGTTGCC TGGAGAAGAT GCATGCCAGT GCCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530558.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528860.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA.

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CloneID	OHu53768
Clone ID Related Accession (Same CDS sequence)	NM_001363852.1 , NM_001363852.2 , XM_011530558.2 , NM_001364320.1 , XM_017029110.1
Accession Version	XM_011530558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528860.1
CDS	210..4955
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530558.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530558.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029110.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884599.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu53768
Clone ID Related Accession (Same CDS sequence)	NM_001363852.1 , NM_001363852.2 , XM_011530558.2 , NM_001364320.1 , XM_017029110.1
Accession Version	XM_017029110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884599.1
CDS	107..4852
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_017029110.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029110.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGTATCT GTGGACCAAA CAGCCACTCC TATGTTGGAC 
GGCACCAGTT TGGGCATATG CACAGGCCAA TCCATGGACA GAGGCAACAG CCAGACCTTT 
GGGAACTCCC AGAACATAGG AGAACAGGGC TACTCCTCCA CAAACTCCAG TGACAGCAGC 
TCTCAGCAGC TGGTGGCAAG CTCCTTGACC TCATCCTCTA CCCTGACAGA GATCCTGGAA 
GCCATGAAGC ACCCCTCGAC AGGAGTCCAG CTGCTCTCTG AACAGAAGGG CCTCTCACCG 
TACTGCTTCA TCAGCGCGGA GGTGGTACAC TGGTTGGTGA ACCACGTGGA GGGGATCCAG 
ACACAGGCGA TGGCCATTGA CATCATGCAG AAAATGCTGG AAGAGCAGCT CATCACACAT 
GCATCTGGCG AAGCCTGGCG GACCTTCATC TACGGCTTCT ATTTCTACAA GATAGTAACG 
GACAAAGAGC CCGACCGAGT GGCCATGCAG CAGCCCGCCA CCACCTGGCA CACAGCAGGA 
GTGGACGACT TCGCCAGCTT CCAGCGCAAG TGGTTTGAGG TGGCCTTTGT GGCAGAAGAG 
CTCGTGCACT CTGAGATTCC TGCCTTTCTC CTGCCCTGGC TGCCTAGCCG GCCAGCCTCC 
TATGCAAGTA GGCACAGCTC CTTTAGCCGA AGTTTTGGAG GACGGAGCCA GGCGGCAGCA 
CTTTTAGCTG CCACTGTCCC AGAGCAGAGG ACTGTGACCC TGGATGTTGA CGTGAACAAC 
CGCACAGACC GGCTGGAGTG GTGCAGCTGT TATTACCATG GCAACTTTTC TCTGAATGCA 
GCCTTTGAGA TCAAGCTGCA CTGGATGGCG GTGACCGCAG CAGTACTCTT CGAGATGGTC 
CAAGGTTGGC ATCGGAAAGC CACCTCCTGT GGCTTCTTGT TAGTCCCAGT TTTGGAGGGG 
CCTTTTGCAC TGCCCAGTTA CCTGTATGGC GACCCCCTTC GTGCCCAGCT CTTCATCCCA 
CTCAACATCA GCTGCTTGCT CAAGGAGGGC AGCGAGCACC TGTTTGATAG CTTTGAACCC 
GAAACGTACT GGGATCGAAT GCACCTCTTC CAGGAAGCCA TTGCACACAG GTTTGGGTTT 
GTACAAGATA AATATTCTGC CTCTGCTTTT AACTTCCCTG CTGAGAACAA GCCTCAGTAT 
ATCCACGTTA CAGGAACAGT GTTTCTGCAG CTGCCCTACT CCAAGCGCAA GTTCTCAGGG 
CAGCAGCGGC GGCGGCGGAA CTCCACCAGC TCCACCAACC AGAACATGTT CTGCGAGGAG 
CGGGTCGGCT ACAACTGGGC CTACAACACC ATGCTCACCA AAACATGGCG CTCCAGCGCC 
ACAGGGGATG AAAAGTTTGC TGATCGGCTG CTGAAGGACT TCACGGACTT CTGCATCAAC 
CGTGACAACC GGCTGGTCAC GTTCTGGACA AGTTGCCTGG AGAAGATGCA TGCCAGTGCC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_017029112.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884601.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu53769
Clone ID Related Accession (Same CDS sequence)	NM_001363854.1 , NM_001364319.1 , XM_017029112.1 , XM_011530560.2 , NM_001363854.2
Accession Version	XM_017029112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884601.1
CDS	107..4684
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_017029112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029112.1

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530560.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528862.1 Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA.

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CloneID	OHu53769
Clone ID Related Accession (Same CDS sequence)	NM_001363854.1 , NM_001364319.1 , XM_017029112.1 , XM_011530560.2 , NM_001363854.2
Accession Version	XM_011530560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DEP domain-containing protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528862.1
CDS	229..4806
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530560.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC 
ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT TACCACCGAC 
TACTTCCCTG ACCGCCAGGG CCTGCAGAAT GACTACACAG AGGGCTGTTA TGATCTCCTT 
CCAGAAGCAG ACATCGACAG GAGGGACGAA GATGGTGTGC AGATGACAGC CCAGCAGGTA 
TTTGAAGAGT TTATTTGCCA ACGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG 
ACACAGAAAC CCAATCCTGC TGTCCCGCCC CCGCTGAGCA GTAGCCCACT CTATAGCCGA 
GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAGT ATTGGCTGAG TATGGGCAGA 
ACGTTCCACA AAGTGACGCT GAAGGATAAG ATGATCACAG TGACGCGATA CCTTCCCAAG 
TATCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGCC TCTGTCCTTC CCACTCAGAC 
TCAGAGTTCG TCTCCTGCTG GGTGGAATTC TCCCACGAAC GGCTGGAGGA GTACAAGTGG 
AATTACTTAG ATCAGTATAT CTGTTCTGCC GGCTCTGAAG ACTTCAGCTT AATTGAGTCC 
CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACCGC CACCAAGCGC 
ATCACGGAGG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCCCGTGC AGACGAGGAC 
GAGTGGCAAC TCCTGGATGG TTTTGTCCGC TTTGTGGAGG GCTTGAATCG CATTCGCAGG 
CGGCATCGCT CGGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGCTT GCAGATGACT 
GGGCCCATTT CCACGCATTC TCTGGAGTCA ACTGCACCCC CAGTGGGGAA GAAGGGAACC 
TCAGCTCTCT CTGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTGGG AGAACAGCAG 
GCAGCTGTGC ATGGTGGGAA GAGCTCCGCC CAGTCAGCCG AGAGCAGCAG CGTTGCCATG 
ACTCCCACCT ACATGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC 
AGCCCACGCA CAGCATCGTC CGCCTTCTAC CCTCAGGTAT CTGTGGACCA AACAGCCACT 
CCTATGTTGG ACGGCACCAG TTTGGGCATA TGCACAGGCC AATCCATGGA CAGAGGCAAC 
AGCCAGACCT TTGGGAACTC CCAGAACATA GGAGAACAGG GCTACTCCTC CACAAACTCC 
AGTGACAGCA GCTCTCAGCA GCTGGTGGCA AGCTCCTTGA CCTCATCCTC TACCCTGACA 
GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGAGTCC AGCTGCTCTC TGAACAGAAG 
GGCCTCTCAC CGTACTGCTT CATCAGCGCG GAGGTGGTAC ACTGGTTGGT GAACCACGTG 
GAGGGGATCC AGACACAGGC GATGGCCATT GACATCATGC AGAAAATGCT GGAAGAGCAG 
CTCATCACAC ATGCATCTGG CGAAGCCTGG CGGACCTTCA TCTACGGCTT CTATTTCTAC 
AAGATAGTAA CGGACAAAGA GCCCGACCGA GTGGCCATGC AGCAGCCCGC CACCACCTGG 
CACACAGCAG GAGTGGACGA CTTCGCCAGC TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT 
GTGGCAGAAG AGCTCGTGCA CTCTGAGATT CCTGCCTTTC TCCTGCCCTG GCTGCCTAGC 
CGGCCAGCCT CCTATGCAAG TAGGCACAGC TCCTTTAGCC GAAGTTTTGG AGGACGGAGC 
CAGGCGGCAG CACTTTTAGC TGCCACTGTC CCAGAGCAGA GGACTGTGAC CCTGGATGTT 
GACGTGAACA ACCGCACAGA CCGGCTGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTT 
TCTCTGAATG CAGCCTTTGA GATCAAGCTG CACTGGATGG CGGTGACCGC AGCAGTACTC 
TTCGAGATGG TCCAAGGTTG GCATCGGAAA GCCACCTCCT GTGGCTTCTT GTTAGTCCCA 
GTTTTGGAGG GGCCTTTTGC ACTGCCCAGT TACCTGTATG GCGACCCCCT TCGTGCCCAG 
CTCTTCATCC CACTCAACAT CAGCTGCTTG CTCAAGGAGG GCAGCGAGCA CCTGTTTGAT 
AGCTTTGAAC CCGAAACGTA CTGGGATCGA ATGCACCTCT TCCAGGAAGC CATTGCACAC 
AGGTTTGGGT TTGTACAAGA TAAATATTCT GCCTCTGCTT TTAACTTCCC TGCTGAGAAC 
AAGCCTCAGT ATATCCACGT TACAGGAACA GTGTTTCTGC AGCTGCCCTA CTCCAAGCGC 
AAGTTCTCAG GGCAGCAGCG GCGGCGGCGG AACTCCACCA GCTCCACCAA CCAGAACATG 
TTCTGCGAGG AGCGGGTCGG CTACAACTGG GCCTACAACA CCATGCTCAC CAAAACATGG 
CGCTCCAGCG CCACAGGGGA TGAAAAGTTT GCTGATCGGC TGCTGAAGGA CTTCACGGAC 
TTCTGCATCA ACCGTGACAA CCGGCTGGTC ACGTTCTGGA CAAGTTGCCT GGAGAAGATG 
CATGCCAGTG CCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530561.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528863.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X10, mRNA.

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CloneID	OHu53770
Clone ID Related Accession (Same CDS sequence)	XM_011530561.2
Accession Version	XM_011530561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC 
ACTACTCCGG CGTGCCTCCC CCTTACCACC GACTACTTCC CTGACCGCCA GGGCCTGCAG 
AATGACTACA CAGAGGGCTG TTATGATCTC CTTCCAGAAG CAGACATCGA CAGGAGGGAC 
GAAGATGGTG TGCAGATGAC AGCCCAGCAG GTATTTGAAG AGTTTATTTG CCAACGTCTC 
ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGACACAGA AACCCAATCC TGCTGTCCCG 
CCCCCGCTGA GCAGTAGCCC ACTCTATAGC CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG 
GAGGAGGACC AGTATTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAGGAT 
AAGATGATCA CAGTGACGCG ATACCTTCCC AAGTATCCTT ATGAATCTGC CCAGATCCAC 
TACACCTACA GCCTCTGTCC TTCCCACTCA GACTCAGAGT TCGTCTCCTG CTGGGTGGAA 
TTCTCCCACG AACGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT 
GCCGGCTCTG AAGACTTCAG CTTAATTGAG TCCCTGAAGT TCTGGAGGAC CCGCTTCCTG 
CTGCTGCCAG CCTGTGTCAC CGCCACCAAG CGCATCACGG AGGGGGAGGC CCACTGCGAC 
ATCTATGGGG ACAGGCCCCG TGCAGACGAG GACGAGTGGC AACTCCTGGA TGGTTTTGTC 
CGCTTTGTGG AGGGCTTGAA TCGCATTCGC AGGCGGCATC GCTCGGATCG CATGATGCGG 
AAAGGGACCG CCATGAAAGG CTTGCAGATG ACTGGGCCCA TTTCCACGCA TTCTCTGGAG 
TCAACTGCAC CCCCAGTGGG GAAGAAGGGA ACCTCAGCTC TCTCTGCCCT GTTGGAGATG 
GAGGCCAGTC AGAAGTGCCT GGGAGAACAG CAGGCAGCTG TGCATGGTGG GAAGAGCTCC 
GCCCAGTCAG CCGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACATGGA CAGCCCACGA 
AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCAC GCACAGCATC GTCCGCCTTC 
TACCCTCAGG TATCTGTGGA CCAAACAGCC ACTCCTATGT TGGACGGCAC CAGTTTGGGC 
ATATGCACAG GCCAATCCAT GGACAGAGGC AACAGCCAGA CCTTTGGGAA CTCCCAGAAC 
ATAGGAGAAC AGGGCTACTC CTCCACAAAC TCCAGTGACA GCAGCTCTCA GCAGCTGGTG 
GCAAGCTCCT TGACCTCATC CTCTACCCTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC 
TCGACAGGAG TCCAGCTGCT CTCTGAACAG AAGGGCCTCT CACCGTACTG CTTCATCAGC 
GCGGAGGTGG TACACTGGTT GGTGAACCAC GTGGAGGGGA TCCAGACACA GGCGATGGCC 
ATTGACATCA TGCAGAAAAT GCTGGAAGAG CAGCTCATCA CACATGCATC TGGCGAAGCC 
TGGCGGACCT TCATCTACGG CTTCTATTTC TACAAGATAG TAACGGACAA AGAGCCCGAC 
CGAGTGGCCA TGCAGCAGCC CGCCACCACC TGGCACACAG CAGGAGTGGA CGACTTCGCC 
AGCTTCCAGC GCAAGTGGTT TGAGGTGGCC TTTGTGGCAG AAGAGCTCGT GCACTCTGAG 
ATTCCTGCCT TTCTCCTGCC CTGGCTGCCT AGCCGGCCAG CCTCCTATGC AAGTAGGCAC 
AGCTCCTTTA GCCGAAGTTT TGGAGGACGG AGCCAGGCGG CAGCACTTTT AGCTGCCACT 
GTCCCAGAGC AGAGGACTGT GACCCTGGAT GTTGACGTGA ACAACCGCAC AGACCGGCTG 
GAGTGGTGCA GCTGTTATTA CCATGGCAAC TTTTCTCTGA ATGCAGCCTT TGAGATCAAG 
CTGCACTGGA TGGCGGTGAC CGCAGCAGTA CTCTTCGAGA TGGTCCAAGG TTGGCATCGG 
AAAGCCACCT CCTGTGGCTT CTTGTTAGTC CCAGTTTTGG AGGGGCCTTT TGCACTGCCC 
AGTTACCTGT ATGGCGACCC CCTTCGTGCC CAGCTCTTCA TCCCACTCAA CATCAGCTGC 
TTGCTCAAGG AGGGCAGCGA GCACCTGTTT GATAGCTTTG AACCCGAAAC GTACTGGGAT 
CGAATGCACC TCTTCCAGGA AGCCATTGCA CACAGGTTTG GGTTTGTACA AGATAAATAT 
TCTGCCTCTG CTTTTAACTT CCCTGCTGAG AACAAGCCTC AGTATATCCA CGTTACAGGA 
ACAGTGTTTC TGCAGCTGCC CTACTCCAAG CGCAAGTTCT CAGGGCAGCA GCGGCGGCGG 
CGGAACTCCA CCAGCTCCAC CAACCAGAAC ATGTTCTGCG AGGAGCGGGT CGGCTACAAC 
TGGGCCTACA ACACCATGCT CACCAAAACA TGGCGCTCCA GCGCCACAGG GGATGAAAAG 
TTTGCTGATC GGCTGCTGAA GGACTTCACG GACTTCTGCA TCAACCGTGA CAACCGGCTG 
GTCACGTTCT GGACAAGTTG CCTGGAGAAG ATGCATGCCA GTGCCCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528863.1
CDS	210..4760
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGMNPRTQNKDSLEDSVSTSPDPMPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMVQGWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNISCLLKEGSEHLFDSFEPETYWDRMHLFQEAIAHRFGFVQDKYSASAFNFPAENKPQYIHVTGTVFLQLPYSKRKFSGQQRRRRNSTSSTNQNMFCEERVGYNWAYNTMLTKTWRSSATGDEKFADRLLKDFTDFCINRDNRLVTFWTSCLEKMHASAP

Target ORF information:

RefSeq Version	XM_011530561.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530561.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GTATGAATCC TAGGACCCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT 
ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC 
ACTACTCCGG CGTGCCTCCC CCTTACCACC GACTACTTCC CTGACCGCCA GGGCCTGCAG 
AATGACTACA CAGAGGGCTG TTATGATCTC CTTCCAGAAG CAGACATCGA CAGGAGGGAC 
GAAGATGGTG TGCAGATGAC AGCCCAGCAG GTATTTGAAG AGTTTATTTG CCAACGTCTC 
ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGACACAGA AACCCAATCC TGCTGTCCCG 
CCCCCGCTGA GCAGTAGCCC ACTCTATAGC CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG 
GAGGAGGACC AGTATTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAGGAT 
AAGATGATCA CAGTGACGCG ATACCTTCCC AAGTATCCTT ATGAATCTGC CCAGATCCAC 
TACACCTACA GCCTCTGTCC TTCCCACTCA GACTCAGAGT TCGTCTCCTG CTGGGTGGAA 
TTCTCCCACG AACGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT 
GCCGGCTCTG AAGACTTCAG CTTAATTGAG TCCCTGAAGT TCTGGAGGAC CCGCTTCCTG 
CTGCTGCCAG CCTGTGTCAC CGCCACCAAG CGCATCACGG AGGGGGAGGC CCACTGCGAC 
ATCTATGGGG ACAGGCCCCG TGCAGACGAG GACGAGTGGC AACTCCTGGA TGGTTTTGTC 
CGCTTTGTGG AGGGCTTGAA TCGCATTCGC AGGCGGCATC GCTCGGATCG CATGATGCGG 
AAAGGGACCG CCATGAAAGG CTTGCAGATG ACTGGGCCCA TTTCCACGCA TTCTCTGGAG 
TCAACTGCAC CCCCAGTGGG GAAGAAGGGA ACCTCAGCTC TCTCTGCCCT GTTGGAGATG 
GAGGCCAGTC AGAAGTGCCT GGGAGAACAG CAGGCAGCTG TGCATGGTGG GAAGAGCTCC 
GCCCAGTCAG CCGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACATGGA CAGCCCACGA 
AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCAC GCACAGCATC GTCCGCCTTC 
TACCCTCAGG TATCTGTGGA CCAAACAGCC ACTCCTATGT TGGACGGCAC CAGTTTGGGC 
ATATGCACAG GCCAATCCAT GGACAGAGGC AACAGCCAGA CCTTTGGGAA CTCCCAGAAC 
ATAGGAGAAC AGGGCTACTC CTCCACAAAC TCCAGTGACA GCAGCTCTCA GCAGCTGGTG 
GCAAGCTCCT TGACCTCATC CTCTACCCTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC 
TCGACAGGAG TCCAGCTGCT CTCTGAACAG AAGGGCCTCT CACCGTACTG CTTCATCAGC 
GCGGAGGTGG TACACTGGTT GGTGAACCAC GTGGAGGGGA TCCAGACACA GGCGATGGCC 
ATTGACATCA TGCAGAAAAT GCTGGAAGAG CAGCTCATCA CACATGCATC TGGCGAAGCC 
TGGCGGACCT TCATCTACGG CTTCTATTTC TACAAGATAG TAACGGACAA AGAGCCCGAC 
CGAGTGGCCA TGCAGCAGCC CGCCACCACC TGGCACACAG CAGGAGTGGA CGACTTCGCC 
AGCTTCCAGC GCAAGTGGTT TGAGGTGGCC TTTGTGGCAG AAGAGCTCGT GCACTCTGAG 
ATTCCTGCCT TTCTCCTGCC CTGGCTGCCT AGCCGGCCAG CCTCCTATGC AAGTAGGCAC 
AGCTCCTTTA GCCGAAGTTT TGGAGGACGG AGCCAGGCGG CAGCACTTTT AGCTGCCACT 
GTCCCAGAGC AGAGGACTGT GACCCTGGAT GTTGACGTGA ACAACCGCAC AGACCGGCTG 
GAGTGGTGCA GCTGTTATTA CCATGGCAAC TTTTCTCTGA ATGCAGCCTT TGAGATCAAG 
CTGCACTGGA TGGCGGTGAC CGCAGCAGTA CTCTTCGAGA TGGTCCAAGG TTGGCATCGG 
AAAGCCACCT CCTGTGGCTT CTTGTTAGTC CCAGTTTTGG AGGGGCCTTT TGCACTGCCC 
AGTTACCTGT ATGGCGACCC CCTTCGTGCC CAGCTCTTCA TCCCACTCAA CATCAGCTGC 
TTGCTCAAGG AGGGCAGCGA GCACCTGTTT GATAGCTTTG AACCCGAAAC GTACTGGGAT 
CGAATGCACC TCTTCCAGGA AGCCATTGCA CACAGGTTTG GGTTTGTACA AGATAAATAT 
TCTGCCTCTG CTTTTAACTT CCCTGCTGAG AACAAGCCTC AGTATATCCA CGTTACAGGA 
ACAGTGTTTC TGCAGCTGCC CTACTCCAAG CGCAAGTTCT CAGGGCAGCA GCGGCGGCGG 
CGGAACTCCA CCAGCTCCAC CAACCAGAAC ATGTTCTGCG AGGAGCGGGT CGGCTACAAC 
TGGGCCTACA ACACCATGCT CACCAAAACA TGGCGCTCCA GCGCCACAGG GGATGAAAAG 
TTTGCTGATC GGCTGCTGAA GGACTTCACG GACTTCTGCA TCAACCGTGA CAACCGGCTG 
GTCACGTTCT GGACAAGTTG CCTGGAGAAG ATGCATGCCA GTGCCCCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEPDC5 ( XM_011530562.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528864.1 Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X11, mRNA.

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CloneID	OHu63148
Clone ID Related Accession (Same CDS sequence)	XM_011530562.2
Accession Version	XM_011530562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GATOR complex protein DEPDC5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGCTTTGA ACCCGAAACG TACTGGGATC GAATGCACCT CTTCCAGGAA GCCATTGCAC 
ACAGGTTTGG GTTTGTACAA GATAAATATT CTGCCTCTGC TTTTAACTTC CCTGCTGAGA 
ACAAGCCTCA GTATATCCAC GTTACAGGAA CAGTGTTTCT GCAGCTGCCC TACTCCAAGC 
GCAAGTTCTC AGGGCAGCAG CGGCGGCGGC GGAACTCCAC CAGCTCCACC AACCAGAACA 
TGTTCTGCGA GGAGCGGGTC GGCTACAACT GGGCCTACAA CACCATGCTC ACCAAAACAT 
GGCGCTCCAG CGCCACAGGG GATGAAAAGT TTGCTGATCG GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528864.1
CDS	210..4757
Translation	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVDQTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQAGELWVKNEKVMCGYISEDTRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEKNCSHEVTVVLFSRTFYDAKSVDEFPEINRASIRQDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQYPVLVRLEQAEGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSAPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFTPRIKLAGKKPASEKAKNGRDTSLGSPKESENALPIQVDYDAYDAQVFRLPGPSRAQCLTTCRSVRERESHSRKSASSCDVSSSPSLPSRTLPTEEVRSQASDDSSLGKSANILMIPHPHLHQYEVSSSLGYTSTRDVLENMMEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAIQIHHQTRQNMAELQGSGQRDPTHSSAELLELAYHEAAGRHSNSRQPGDGMSFLNFSGTEELSVGLLSNSGAGMNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYTEGCYDLLPEADIDRRDEDGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNPAVPPPLSSSPLYSRGLVSRNRPEEEDQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVEFSHERLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEAHCDIYGDRPRADEDEWQLLDGFVRFVEGLNRIRRRHRSDRMMRKGTAMKGLQMTGPISTHSLESTAPPVGKKGTSALSALLEMEASQKCLGEQQAAVHGGKSSAQSAESSSVAMTPTYMDSPRKDGAFFMEFVRSPRTASSAFYPQVSVDQTATPMLDGTSLGICTGQSMDRGNSQTFGNSQNIGEQGYSSTNSSDSSSQQLVASSLTSSSTLTEILEAMKHPSTGVQLLSEQKGLSPYCFISAEVVHWLVNHVEGIQTQAMAIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVTDKEPDRVAMQQPATTWHTAGVDDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRSFGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTAAVLFEMALNPKRTGIECTSSRKPLHTGLGLYKINILPLLLTSLLRTSLSISTLQEQCFCSCPTPSASSQGSSGGGGTPPAPPTRTCSARSGSATTGPTTPCSPKHGAPAPQGMKSLLIGC

Target ORF information:

RefSeq Version	XM_011530562.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530562.2

ORF Insert Sequence:

ATGAGAACAA CAAAGGTCTA CAAACTCGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
GATGAGCTAG TTGTGAACCC CAAAGTGTTC CCTCACATCA AGCTTGGAGA CATTGTAGAG 
ATTGCACACC CCAACGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC TCTTAAGGAA 
GATTTACAGA AGGAAACTAT CAGTGTGGAC CAGACTGTGA CTCAAGTGTT CCGGCTGAGA 
CCTTATCAGG ATGTCTATGT TAATGTCGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG 
GAATTAACTT TTAAGGATCA GTATATTGGC CGTGGGGATA TGTGGCGACT AAAGAAAAGT 
TTGGTCAGCA CATGTGCCTA TATCACCCAG AAGGTGGAGT TTGCTGGCAT CAGAGCACAG 
GCTGGTGAAC TGTGGGTTAA GAATGAGAAG GTCATGTGTG GCTACATCAG TGAAGATACC 
AGGGTGGTGT TTCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA 
ATGTGGGATT TTGATATTTA TGGGGATTTG TATTTTGAGA AAGCTGTGAA TGGTTTCCTT 
GCTGATCTAT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTG 
TTTTCTAGAA CTTTCTATGA TGCAAAATCT GTTGATGAAT TTCCTGAAAT AAACCGAGCC 
TCAATTCGAC AGGATCACAA GGGGAGATTC TATGAAGACT TTTACAAAGT GGTGGTGCAG 
AATGAGAGAA GAGAAGAATG GACTTCACTT CTCGTAACCA TTAAAAAACT CTTCATCCAG 
TATCCAGTGT TGGTGCGACT GGAACAGGCA GAGGGCTTTC CTCAAGGAGA TAATTCTACC 
TCAGCACAAG GAAACTACCT GGAGGCCATC AATCTGTCAT TCAATGTGTT TGATAAGCAC 
TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCAG TGGTGATCAC GCCCGGGGTG 
GGTGTCTTTG AAGTGGACCG CCTACTCATG ATCCTGACCA AGCAGCGGAT GATAGATAAT 
GGAATTGGTG TGGATTTGGT GTGCATGGGA GAGCAACCGT TACATGCTGT CCCATTGTTC 
AAGCTCCATA ATCGGAGTGC TCCCCGTGAT TCTCGTCTGG GCGATGACTA TAATATCCCT 
CACTGGATAA ACCACAGTTT CTACACATCC AAAAGCCAGC TCTTTTGTAA TAGTTTCACC 
CCACGAATAA AACTGGCAGG AAAGAAGCCC GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT 
ACATCTCTCG GGAGTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAC 
GCCTATGACG CTCAAGTGTT CAGGCTGCCC GGCCCATCCC GGGCCCAGTG CCTCACCACC 
TGCAGATCTG TGCGAGAGCG AGAGAGTCAC AGTCGAAAGA GTGCCAGCTC CTGTGATGTT 
TCATCCAGCC CTTCCCTACC AAGCCGCACA CTGCCCACTG AGGAAGTGAG GAGCCAGGCT 
TCTGACGACA GCTCCCTAGG CAAGAGTGCC AACATCCTGA TGATCCCACA CCCCCACCTG 
CACCAGTATG AAGTCAGCAG CTCCTTGGGA TACACCAGCA CTCGAGATGT CCTGGAGAAC 
ATGATGGAGC CACCACAGCG AGACTCCAGT GCACCAGGGA GGTTTCACGT TGGCAGTGCA 
GAATCCATGC TGCATGTTCG ACCTGGTGGA TACACGCCCC AGAGAGCACT GATTAACCCC 
TTCGCTCCCT CTCGGATGCC CATGAAGCTT ACGTCCAACA GAAGGCGCTG GATGCACACT 
TTTCCTGTGG GGCCATCCGG AGAAGCCATC CAGATCCACC ACCAGACCCG ACAGAATATG 
GCGGAGCTAC AAGGCAGCGG GCAGAGGGAT CCAACTCACT CCTCTGCAGA GCTGCTGGAG 
TTAGCATATC ATGAAGCTGC TGGAAGGCAC AGCAATTCCC GCCAGCCTGG TGACGGCATG 
TCCTTCTTGA ACTTCAGTGG AACAGAGGAG CTTTCTGTCG GCCTGCTTAG CAACAGTGGT 
GCAGGTATGA ATCCTAGGAC CCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT 
CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT 
GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTTACCAC CGACTACTTC 
CCTGACCGCC AGGGCCTGCA GAATGACTAC ACAGAGGGCT GTTATGATCT CCTTCCAGAA 
GCAGACATCG ACAGGAGGGA CGAAGATGGT GTGCAGATGA CAGCCCAGCA GGTATTTGAA 
GAGTTTATTT GCCAACGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGACACAG 
AAACCCAATC CTGCTGTCCC GCCCCCGCTG AGCAGTAGCC CACTCTATAG CCGAGGCCTT 
GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAGTATTGGC TGAGTATGGG CAGAACGTTC 
CACAAAGTGA CGCTGAAGGA TAAGATGATC ACAGTGACGC GATACCTTCC CAAGTATCCT 
TATGAATCTG CCCAGATCCA CTACACCTAC AGCCTCTGTC CTTCCCACTC AGACTCAGAG 
TTCGTCTCCT GCTGGGTGGA ATTCTCCCAC GAACGGCTGG AGGAGTACAA GTGGAATTAC 
TTAGATCAGT ATATCTGTTC TGCCGGCTCT GAAGACTTCA GCTTAATTGA GTCCCTGAAG 
TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CCGCCACCAA GCGCATCACG 
GAGGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCCC GTGCAGACGA GGACGAGTGG 
CAACTCCTGG ATGGTTTTGT CCGCTTTGTG GAGGGCTTGA ATCGCATTCG CAGGCGGCAT 
CGCTCGGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GCTTGCAGAT GACTGGGCCC 
ATTTCCACGC ATTCTCTGGA GTCAACTGCA CCCCCAGTGG GGAAGAAGGG AACCTCAGCT 
CTCTCTGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TGGGAGAACA GCAGGCAGCT 
GTGCATGGTG GGAAGAGCTC CGCCCAGTCA GCCGAGAGCA GCAGCGTTGC CATGACTCCC 
ACCTACATGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCA 
CGCACAGCAT CGTCCGCCTT CTACCCTCAG GTATCTGTGG ACCAAACAGC CACTCCTATG 
TTGGACGGCA CCAGTTTGGG CATATGCACA GGCCAATCCA TGGACAGAGG CAACAGCCAG 
ACCTTTGGGA ACTCCCAGAA CATAGGAGAA CAGGGCTACT CCTCCACAAA CTCCAGTGAC 
AGCAGCTCTC AGCAGCTGGT GGCAAGCTCC TTGACCTCAT CCTCTACCCT GACAGAGATC 
CTGGAAGCCA TGAAGCACCC CTCGACAGGA GTCCAGCTGC TCTCTGAACA GAAGGGCCTC 
TCACCGTACT GCTTCATCAG CGCGGAGGTG GTACACTGGT TGGTGAACCA CGTGGAGGGG 
ATCCAGACAC AGGCGATGGC CATTGACATC ATGCAGAAAA TGCTGGAAGA GCAGCTCATC 
ACACATGCAT CTGGCGAAGC CTGGCGGACC TTCATCTACG GCTTCTATTT CTACAAGATA 
GTAACGGACA AAGAGCCCGA CCGAGTGGCC ATGCAGCAGC CCGCCACCAC CTGGCACACA 
GCAGGAGTGG ACGACTTCGC CAGCTTCCAG CGCAAGTGGT TTGAGGTGGC CTTTGTGGCA 
GAAGAGCTCG TGCACTCTGA GATTCCTGCC TTTCTCCTGC CCTGGCTGCC TAGCCGGCCA 
GCCTCCTATG CAAGTAGGCA CAGCTCCTTT AGCCGAAGTT TTGGAGGACG GAGCCAGGCG 
GCAGCACTTT TAGCTGCCAC TGTCCCAGAG CAGAGGACTG TGACCCTGGA TGTTGACGTG 
AACAACCGCA CAGACCGGCT GGAGTGGTGC AGCTGTTATT ACCATGGCAA CTTTTCTCTG 
AATGCAGCCT TTGAGATCAA GCTGCACTGG ATGGCGGTGA CCGCAGCAGT ACTCTTCGAG 
ATGGCTTTGA ACCCGAAACG TACTGGGATC GAATGCACCT CTTCCAGGAA GCCATTGCAC 
ACAGGTTTGG GTTTGTACAA GATAAATATT CTGCCTCTGC TTTTAACTTC CCTGCTGAGA 
ACAAGCCTCA GTATATCCAC GTTACAGGAA CAGTGTTTCT GCAGCTGCCC TACTCCAAGC 
GCAAGTTCTC AGGGCAGCAG CGGCGGCGGC GGAACTCCAC CAGCTCCACC AACCAGAACA 
TGTTCTGCGA GGAGCGGGTC GGCTACAACT GGGCCTACAA CACCATGCTC ACCAAAACAT 
GGCGCTCCAG CGCCACAGGG GATGAAAAGT TTGCTGATCG GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DEPDC5 deficiency contributes to resistance to leucine starvation via p62 accumulation in hepatocellular carcinoma.
Scientific reports8(1)106(2018 Jan)
Mizuno Y,Shimada S,Akiyama Y,Watanabe S,Aida T,Ogawa K,Ono H,Mitsunori Y,Ban D,Kudo A,Arii S,Yamaoka S,Tanabe M,Tanaka S

KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
Nature543(7645)438-442(2017 03)
Wolfson RL,Chantranupong L,Wyant GA,Gu X,Orozco JM,Shen K,Condon KJ,Petri S,Kedir J,Scaria SM,Abu-Remaileh M,Frankel WN,Sabatini DM

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DEPDC5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: