gRNAs
The following TGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu20166 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_024831.6 , NM_024831.7 , NM_024831.8 | |
| Accession Version | NM_024831.6 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2015-03-14 | |
| Organism | Homo sapiens(Human) | |
| Product | trimethylguanosine synthase | |
| Comment | ||
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_079107.6 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_024831.6 |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu20166 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_024831.6 , NM_024831.7 , NM_024831.8 | |
| Accession Version | NM_024831.7 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-25 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, AC100817.5, DB082992.1, AF432215.1, AF286340.1 and AC018607.12. On Dec 13, 2015 this sequence version replaced NM_024831.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.246294.1, SRR1660809.44368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_079107.6 |
| CDS | 388..2949 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_024831.7 |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu20166 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_024831.6 , NM_024831.7 , NM_024831.8 | |
| Accession Version | NM_024831.8 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-10 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, AF432215.1, AF286340.1 and AC018607.12. On May 17, 2019 this sequence version replaced NM_024831.7. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.246294.1, SRR1803615.245414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260129.6/ ENSP00000260129.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_079107.6 |
| CDS | 174..2735 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_024831.8 |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42323 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001317902.1 , NM_001317902.2 | |
| Accession Version | NM_001317902.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-06-30 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, AC100817.5, BX648548.1, AF286340.1 and AC018607.12. On Dec 12, 2015 this sequence version replaced XM_006716486.2. Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in the use of a downstream translation start site, a frameshift and an early stop codon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001304831.1 |
| CDS | 602..2542 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001317902.1 |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42323 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001317902.1 , NM_001317902.2 | |
| Accession Version | NM_001317902.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC100817.5, BX648548.1, AF286340.1 and AC018607.12. On May 31, 2019 this sequence version replaced NM_001317902.1. Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in the use of a downstream translation start site, a frameshift and an early stop codon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001304831.1 |
| CDS | 388..2328 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001317902.2 |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42321 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005251328.2 , NM_001363184.1 , NM_001363184.2 | |
| Accession Version | NM_001363184.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-07-01 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, DA456243.1, AF432215.1, AF286340.1 and AC018607.12. On May 11, 2018 this sequence version replaced XM_005251328.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.134002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001350113.1 |
| CDS | 388..2670 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001363184.1 |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42321 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005251328.2 , NM_001363184.1 , NM_001363184.2 | |
| Accession Version | NM_001363184.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-10 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, DA456243.1, AF432215.1, AF286340.1 and AC018607.12. On May 31, 2019 this sequence version replaced NM_001363184.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.134002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001350113.1 |
| CDS | 388..2670 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001363184.2 |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42321 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005251328.2 , NM_001363184.1 , NM_001363184.2 | |
| Accession Version | XM_005251328.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005251385.1 |
| CDS | 382..2664 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005251328.2 |
1 | ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu42322 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006716485.2 | |
| Accession Version | XM_006716485.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006716548.1 |
| CDS | 174..2393 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006716485.2 |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu98310 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017014004.1 | |
| Accession Version | XM_017014004.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | trimethylguanosine synthase isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016869493.1 |
| CDS | 174..2372 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017014004.1 |
1 | ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Architecture of the human interactome defines protein communities and disease networks. |
The enzyme L-isoaspartyl (D-aspartyl) methyltransferase is required for VEGF-dependent endothelial cell migration and tubulogenesis. |
Self-association of Trimethylguanosine Synthase Tgs1 is required for efficient snRNA/snoRNA trimethylation and pre-rRNA processing. |
Genome-wide association study identifies a novel susceptibility gene for serum TSH levels in Chinese populations. |
PIMT prevents the apoptosis of endothelial cells in response to glycated low density lipoproteins and protective effects of grape seed procyanidin B2. |
