TGS1 cDNA ORF clone, Homo sapiens(human)

The following TGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20166 NM_024831.8
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$429.50-$601.30
$859.00
OHu42323 NM_001317902.2
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu42321 NM_001363184.2
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu42321 XM_005251328.2
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu42322 XM_006716485.2
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu98310 XM_017014004.1
Latest version!
Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu42323 NM_001317902.1 Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu42321 NM_001363184.1 Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu20166 NM_024831.6 Homo sapiens trimethylguanosine synthase 1 (TGS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$429.50-$601.30
$859.00
OHu20166 NM_024831.7 Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$429.50-$601.30
$859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20166
    Clone ID Related Accession (Same CDS sequence) NM_024831.6 , NM_024831.7 , NM_024831.8
    Accession Version NM_024831.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product trimethylguanosine synthase
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079107.6
    Translation

    Target ORF information:

    RefSeq Version NM_024831.6
    Organism Homo sapiens(Human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024831.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20166
    Clone ID Related Accession (Same CDS sequence) NM_024831.6 , NM_024831.7 , NM_024831.8
    Accession Version NM_024831.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-25
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, AC100817.5, DB082992.1, AF432215.1, AF286340.1 and AC018607.12. On Dec 13, 2015 this sequence version replaced NM_024831.6. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.246294.1, SRR1660809.44368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079107.6
    CDS388..2949
    Translation

    Target ORF information:

    RefSeq Version NM_024831.7
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024831.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20166
    Clone ID Related Accession (Same CDS sequence) NM_024831.6 , NM_024831.7 , NM_024831.8
    Accession Version NM_024831.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, AF432215.1, AF286340.1 and AC018607.12. On May 17, 2019 this sequence version replaced NM_024831.7. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.246294.1, SRR1803615.245414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260129.6/ ENSP00000260129.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079107.6
    CDS174..2735
    Translation

    Target ORF information:

    RefSeq Version NM_024831.8
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024831.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGTGATTGC CATTGATATC
    GATCCTGTTA AGATTGCCCT TGCTCGCAAT AATGCAGAAG TTTATGGGAT AGCAGATAAG
    ATAGAGTTCA TCTGTGGAGA TTTCTTGCTG CTGGCTTCTT TTTTAAAGGC TGATGTTGTG
    TTCCTCAGCC CACCTTGGGG AGGGCCAGAC TATGCCACTG CAGAGACCTT TGACATTAGA
    ACAATGATGT CTCCTGATGG CTTTGAAATT TTCAGACTTT CTAAGAAGAT CACTAATAAT
    ATTGTTTATT TTCTTCCAAG AAATGCTGAT ATTGACCAGG TGGCATCCTT AGCTGGGCCT
    GGAGGGCAAG TGGAAATAGA ACAGAACTTC CTTAACAACA AATTGAAGAC AATCACTGCA
    TATTTTGGTG ACCTAATTCG AAGACCAGCC TCTGAAACCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42323
    Clone ID Related Accession (Same CDS sequence) NM_001317902.1 , NM_001317902.2
    Accession Version NM_001317902.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, AC100817.5, BX648548.1, AF286340.1 and AC018607.12. On Dec 12, 2015 this sequence version replaced XM_006716486.2. Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in the use of a downstream translation start site, a frameshift and an early stop codon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGACTTTC TAAGAAGATC ACTAATAATA TTGTTTATTT TCTTCCAAGA AATGCTGATA
    TTGACCAGGT GGCATCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304831.1
    CDS602..2542
    Translation

    Target ORF information:

    RefSeq Version NM_001317902.1
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGACTTTC TAAGAAGATC ACTAATAATA TTGTTTATTT TCTTCCAAGA AATGCTGATA
    TTGACCAGGT GGCATCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42323
    Clone ID Related Accession (Same CDS sequence) NM_001317902.1 , NM_001317902.2
    Accession Version NM_001317902.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC100817.5, BX648548.1, AF286340.1 and AC018607.12. On May 31, 2019 this sequence version replaced NM_001317902.1. Transcript Variant: This variant (2) has multiple differences compared to variant 1. These differences result in the use of a downstream translation start site, a frameshift and an early stop codon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGACTTTC TAAGAAGATC ACTAATAATA TTGTTTATTT TCTTCCAAGA AATGCTGATA
    TTGACCAGGT GGCATCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001304831.1
    CDS388..2328
    Translation

    Target ORF information:

    RefSeq Version NM_001317902.2
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001317902.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGACTTTC TAAGAAGATC ACTAATAATA TTGTTTATTT TCTTCCAAGA AATGCTGATA
    TTGACCAGGT GGCATCCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42321
    Clone ID Related Accession (Same CDS sequence) XM_005251328.2 , NM_001363184.1 , NM_001363184.2
    Accession Version NM_001363184.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-07-01
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, DA456243.1, AF432215.1, AF286340.1 and AC018607.12. On May 11, 2018 this sequence version replaced XM_005251328.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.134002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350113.1
    CDS388..2670
    Translation

    Target ORF information:

    RefSeq Version NM_001363184.1
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363184.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42321
    Clone ID Related Accession (Same CDS sequence) XM_005251328.2 , NM_001363184.1 , NM_001363184.2
    Accession Version NM_001363184.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-10
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM853546.1, DB082992.1, DA456243.1, AF432215.1, AF286340.1 and AC018607.12. On May 31, 2019 this sequence version replaced NM_001363184.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.134002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350113.1
    CDS388..2670
    Translation

    Target ORF information:

    RefSeq Version NM_001363184.2
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42321
    Clone ID Related Accession (Same CDS sequence) XM_005251328.2 , NM_001363184.1 , NM_001363184.2
    Accession Version XM_005251328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005251328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005251385.1
    CDS382..2664
    Translation

    Target ORF information:

    RefSeq Version XM_005251328.2
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005251328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGAAGTA TGGGATTGCC ACTTCAATTT GGTAGGATAA CTGCACATAA GGATTTTGAG 
    GTATCTATGA ATACTAGAAA TAAAGTTAAA ATAAAAAAGA AAAAACATCA AAAGAAATAC
    TTAGATGAAA TTGTGCAAGA ATCTTGGAGA AAAGAATATG AAGAAGACGA CATTTTGGCT
    TCAGATGATC CATCTTCAAT TGAACAGTAT GAGAACACCA GAACATATGA ACTTCAAAGC
    AAAAAAGATA CTGAGACAGA AAATCCTCCA GTTGAAAACA CATTATCTCC AAAGCTAGAA
    ATTACAGAGA AATGGGAAAA GTATTGGAAT GAATATGGAG GAGGACTATT GTGGCAAAGT
    TGGCAAGAAA AACATCCGGG TCAAGCACTA TCTTCTGAAC CTTGGAACTT TCCTGATACA
    AAGGAAGAAT GGGAGCAACA TTATAGTCAA CTTTATTGGT ATTATTTGGA ACAATTTCAG
    TATTGGGAAG CTCAGGGTTG GACTTTTGAT GCCTCGCAAA GCTGTGATAC AGATACTTAC
    ACATCTAAAA CAGAAGCTGA TGACAAGAAC GATGAAAAAT GCATGAAAGT TGACTTAGTA
    TCTTTTCCAT CTTCACCTAT TATGGTTGAT AATGATAGCT CTGGTACAAG TGATAAGGAT
    CATAGTGAAA TACTTGATGG AATTAGTAAC ATAAAACTGA ATTCAGAGGA AGTAACACAG
    AGCCAATTAG ATTCCTGTAC AAGTCATGAT GGTCATCAAC AGCTAAGTGA AGTTAGTAGC
    AAAAGAGAGT GCCCTGCTTC CGGCCAAAGT GAACCACGTA ATGGAGGAAC CAATGAGGAA
    AGCAACTCAT CGGGGAATAC AAACACAGAC CCACCAGCTG AGGATTCACA GAAGTCTTCA
    GGAGCAAACA CAAGCAAAGA CAGACCACAT GCCAGTGGTA CTGATGGAGA TGAAAGTGAG
    GAAGACCCAC CTGAGCATAA GCCAAGCAAA CTGAAGAGGA GCCATGAACT GGACATTGAT
    GAAAACCCAG CTTCAGACTT TGATGACAGT GGTTCCCTTC TAGGATTCAA GTATGGCTCA
    GGACAAAAAT ATGGTGGAAT CCCAAATTTC AGTCATCGGC AGGTCAGGTA TTTAGAGAAG
    AATGTGAAGC TTAAGTCTAA GTACCTAGAC ATGCGCAGAC AAATAAAGAT GAAAAACAAA
    CACATCTTCT TTACCAAAGA GTCAGAAAAA CCATTTTTCA AGAAAAGCAA AATTCTGAGT
    AAGGTAGAAA AATTCCTCAC ATGGGTTAAT AAACCAATGG ATGAAGAAGC ATCACAGGAA
    TCATCTTCTC ATGACAATGT GCACGACGCT TCCACAAGTA GTGATTCAGA GGAACAAGAC
    ATGTCTGTTA AAAAAGGTGA TGACCTACTG GAGACTAATA ATCCAGAACC TGAAAAGTGT
    CAGAGCGTAT CTTCAGCTGG TGAACTTGAA ACAGAAAACT ATGAAAGAGA CAGCTTGCTA
    GCAACTGTTC CAGATGAGCA GGATTGTGTT ACTCAAGAAG TGCCAGACTC CCGCCAGGCA
    GAAACTGAAG CTGAAGTGAA AAAGAAGAAG AACAAGAAGA AGAACAAAAA GGTGAATGGT
    CTGCCTCCTG AAATAGCTGC TGTTCCTGAG CTGGCAAAAT ACTGGGCCCA GAGGTACAGG
    CTCTTCTCCC GTTTTGATGA TGGGATTAAG TTGGACAGAG AGGGCTGGTT TTCAGTTACA
    CCCGAGAAGA TTGCTGAACA CATTGCTGGC CGTGTTAGTC AGTCCTTCAA GTGTGACGTT
    GTAGTAGACG CATTCTGTGG AGTTGGAGGA AATACCATTC AGTTTGCCTT AACAGGAATG
    AGAGTGATTG CCATTGATAT CGATCCTGTT AAGATTGCCC TTGCTCGCAA TAATGCAGAA
    GTTTATGGGA TAGCAGATAA GATAGAGTTC ATCTGTGGAG ATTTCTTGCT GCTGGCTTCT
    TTTTTAAAGG CTGATGTTGT GTTCCTCAGC CCACCTTGGG GAGGGCCAGA CTATGCCACT
    GCAGAGACCT TTGACATTAG AACAATGATG TCTCCTGATG GCTTTGAAAT TTTCAGACTT
    TCTAAGAAGA TCACTAATAA TATTGTTTAT TTTCTTCCAA GAAATGCTGA TATTGACCAG
    GTGGCATCCT TAGCTGGGCC TGGAGGGCAA GTGGAAATAG AACAGAACTT CCTTAACAAC
    AAATTGAAGA CAATCACTGC ATATTTTGGT GACCTAATTC GAAGACCAGC CTCTGAAACC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu42322
    Clone ID Related Accession (Same CDS sequence) XM_006716485.2
    Accession Version XM_006716485.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGACTTTCT AAGAAGATCA
    CTAATAATAT TGTTTATTTT CTTCCAAGAA ATGCTGATAT TGACCAGGTG GCATCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006716548.1
    CDS174..2393
    Translation

    Target ORF information:

    RefSeq Version XM_006716485.2
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006716485.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGACTTTCT AAGAAGATCA
    CTAATAATAT TGTTTATTTT CTTCCAAGAA ATGCTGATAT TGACCAGGTG GCATCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98310
    Clone ID Related Accession (Same CDS sequence) XM_017014004.1
    Accession Version XM_017014004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product trimethylguanosine synthase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGGTGGCAT CCTTAGCTGG
    GCCTGGAGGG CAAGTGGAAA TAGAACAGAA CTTCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869493.1
    CDS174..2372
    Translation

    Target ORF information:

    RefSeq Version XM_017014004.1
    Organism Homo sapiens(human)
    Definition Homo sapiens trimethylguanosine synthase 1 (TGS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTGCTGCG AGAAGTGGAG CCGCGTGGCG GAAATGTTTC TCTTCATTGA GGAGCGGGAG 
    GATTGTAAGA TACTGTGCCT TTGCTCCAGG GCATTTGTGG AGGATCGAAA ATTGTACAAT
    TTGGGATTAA AAGGCTATTA CATCAGAGAC AGTGGCAACA ATTCAGGAGA CCAGGCGACA
    GAAGAAGAGG AAGGTGGTTA TTCCTGTGGT ACTGCAGAAT CACATGACAG CAAAGGCATA
    GGCCTGGATG AAAGTGAACT TGATTCTGAG GCTGAACTCA TGAGAAGTAT GGGATTGCCA
    CTTCAATTTG GTAGGATAAC TGCACATAAG GATTTTGAGG TATCTATGAA TACTAGAAAT
    AAAGTTAAAA TAAAAAAGAA AAAACATCAA AAGAAATACT TAGATGAAAT TGTGCAAGAA
    TCTTGGAGAA AAGAATATGA AGAAGACGAC ATTTTGGCTT CAGATGATCC ATCTTCAATT
    GAACAGTATG AGAACACCAG AACATATGAA CTTCAAAGCA AAAAAGATAC TGAGACAGAA
    AATCCTCCAG TTGAAAACAC ATTATCTCCA AAGCTAGAAA TTACAGAGAA ATGGGAAAAG
    TATTGGAATG AATATGGAGG AGGACTATTG TGGCAAAGTT GGCAAGAAAA ACATCCGGGT
    CAAGCACTAT CTTCTGAACC TTGGAACTTT CCTGATACAA AGGAAGAATG GGAGCAACAT
    TATAGTCAAC TTTATTGGTA TTATTTGGAA CAATTTCAGT ATTGGGAAGC TCAGGGTTGG
    ACTTTTGATG CCTCGCAAAG CTGTGATACA GATACTTACA CATCTAAAAC AGAAGCTGAT
    GACAAGAACG ATGAAAAATG CATGAAAGTT GACTTAGTAT CTTTTCCATC TTCACCTATT
    ATGGTTGATA ATGATAGCTC TGGTACAAGT GATAAGGATC ATAGTGAAAT ACTTGATGGA
    ATTAGTAACA TAAAACTGAA TTCAGAGGAA GTAACACAGA GCCAATTAGA TTCCTGTACA
    AGTCATGATG GTCATCAACA GCTAAGTGAA GTTAGTAGCA AAAGAGAGTG CCCTGCTTCC
    GGCCAAAGTG AACCACGTAA TGGAGGAACC AATGAGGAAA GCAACTCATC GGGGAATACA
    AACACAGACC CACCAGCTGA GGATTCACAG AAGTCTTCAG GAGCAAACAC AAGCAAAGAC
    AGACCACATG CCAGTGGTAC TGATGGAGAT GAAAGTGAGG AAGACCCACC TGAGCATAAG
    CCAAGCAAAC TGAAGAGGAG CCATGAACTG GACATTGATG AAAACCCAGC TTCAGACTTT
    GATGACAGTG GTTCCCTTCT AGGATTCAAG TATGGCTCAG GACAAAAATA TGGTGGAATC
    CCAAATTTCA GTCATCGGCA GGTCAGGTAT TTAGAGAAGA ATGTGAAGCT TAAGTCTAAG
    TACCTAGACA TGCGCAGACA AATAAAGATG AAAAACAAAC ACATCTTCTT TACCAAAGAG
    TCAGAAAAAC CATTTTTCAA GAAAAGCAAA ATTCTGAGTA AGGTAGAAAA ATTCCTCACA
    TGGGTTAATA AACCAATGGA TGAAGAAGCA TCACAGGAAT CATCTTCTCA TGACAATGTG
    CACGACGCTT CCACAAGTAG TGATTCAGAG GAACAAGACA TGTCTGTTAA AAAAGGTGAT
    GACCTACTGG AGACTAATAA TCCAGAACCT GAAAAGTGTC AGAGCGTATC TTCAGCTGGT
    GAACTTGAAA CAGAAAACTA TGAAAGAGAC AGCTTGCTAG CAACTGTTCC AGATGAGCAG
    GATTGTGTTA CTCAAGAAGT GCCAGACTCC CGCCAGGCAG AAACTGAAGC TGAAGTGAAA
    AAGAAGAAGA ACAAGAAGAA GAACAAAAAG GTGAATGGTC TGCCTCCTGA AATAGCTGCT
    GTTCCTGAGC TGGCAAAATA CTGGGCCCAG AGGTACAGGC TCTTCTCCCG TTTTGATGAT
    GGGATTAAGT TGGACAGAGA GGGCTGGTTT TCAGTTACAC CCGAGAAGAT TGCTGAACAC
    ATTGCTGGCC GTGTTAGTCA GTCCTTCAAG TGTGACGTTG TAGTAGACGC ATTCTGTGGA
    GTTGGAGGAA ATACCATTCA GTTTGCCTTA ACAGGAATGA GAGGTGGCAT CCTTAGCTGG
    GCCTGGAGGG CAAGTGGAAA TAGAACAGAA CTTCCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.