RASAL2 cDNA ORF clone, Homo sapiens(human)

The following RASAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14676 NM_170692.3
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu90810 XM_017002849.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu90811 XM_017002850.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu90812 XM_017002851.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu90813 XM_017002852.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu90814 XM_017002853.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu90815 XM_017002854.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu90816 XM_017002855.1
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu14551 NM_004841.4
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu34830 XM_005245622.4
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu62841 XM_011510166.2
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu62842 XM_011510167.2
Latest version!
Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu34830 XM_005245622.3 Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu14676 NM_170692.2 Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu14551 NM_004841.3 Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14676
    Clone ID Related Accession (Same CDS sequence) NM_170692.2 , NM_170692.3
    Accession Version NM_170692.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK075169.1, AK311017.1, AL160281.17, BC110611.2, AL035702.7 and AA127237.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2009 this sequence version replaced NM_170692.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.158295.1, BC110611.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GGCCTCTCCC TCGTGTTCTT GCTGATATTA CCAAGTCATT GACTAATCCT
    ACGCCAATAC AACAGCAACT GAGACGCTTC ACTGAACATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT
    AAACTAAAAT CTCCAAGCCA GGACAACACA GACAGCTACT TCAGAGGGAA AACATTATTG
    CTGGTTCAGC AAGCCTCCTC TCAGAGCATG ACTTATTCTG AAAAGGATGA AAGGGAAAGT
    AGCCTTCCTA ATGGTCGGAG CGTCTCCCTC ATGGACCTCC AGGACACTCA TGCTGCTCAA
    GTGGAGCATG CATCTGTCAT GCTTGATGTG CCTATACGCT TGACCGGAAG CCAGCTTTCC
    ATAACCCAGG TGGCCAGCAT CAAACAGCTG CGGGAAACCC AGAGCACTCC CCAAAGTGCA
    CCCCAAGTGA GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGTGGCCT TCAGCCCTTG
    TCGTTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CAGCAATGCC AAAGGCCTCT
    ATAGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA
    GACTTCAAGC TCAGTGGACC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACT
    CAGAGTGAGG ACTTCTCCAG GCGGCACACG GTGCCAGATA GACACATACC TCTTGCTTTG
    CCACGACAAA ATAGTACTGG GCAGGCCCAG ATCCGAAAAG TGGACCAGGG TGGGTTAGGT
    GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CACAGTGCTT CTTTACGTAG CACCGGGAGC
    ATGTCAGTGG TGTCCGCAGC CCTGGTGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCT GTTCCCAAAG TTAGAGCAAT CCAGAGACAA
    CAGACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT
    CTAGATGAAG CCAAGCATGC TGAGAAGTAT GAACAAGAAA TTACTAAACT GAAGGAGCGC
    CTGAGAGTTT CCAGCCGGCG ACTGGAGGAA TATGAACGCC GCTTGCTGGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCCGACTGG AGGACAGCGA GGAGCGGCTC
    CGAAGACAGC AGGAAGAAAA AGATAGCCAG ATGAAAAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAG
    AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCAGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733793.2
    CDS765..4607
    Translation

    Target ORF information:

    RefSeq Version NM_170692.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GGCCTCTCCC TCGTGTTCTT GCTGATATTA CCAAGTCATT GACTAATCCT
    ACGCCAATAC AACAGCAACT GAGACGCTTC ACTGAACATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT
    AAACTAAAAT CTCCAAGCCA GGACAACACA GACAGCTACT TCAGAGGGAA AACATTATTG
    CTGGTTCAGC AAGCCTCCTC TCAGAGCATG ACTTATTCTG AAAAGGATGA AAGGGAAAGT
    AGCCTTCCTA ATGGTCGGAG CGTCTCCCTC ATGGACCTCC AGGACACTCA TGCTGCTCAA
    GTGGAGCATG CATCTGTCAT GCTTGATGTG CCTATACGCT TGACCGGAAG CCAGCTTTCC
    ATAACCCAGG TGGCCAGCAT CAAACAGCTG CGGGAAACCC AGAGCACTCC CCAAAGTGCA
    CCCCAAGTGA GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGTGGCCT TCAGCCCTTG
    TCGTTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CAGCAATGCC AAAGGCCTCT
    ATAGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA
    GACTTCAAGC TCAGTGGACC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACT
    CAGAGTGAGG ACTTCTCCAG GCGGCACACG GTGCCAGATA GACACATACC TCTTGCTTTG
    CCACGACAAA ATAGTACTGG GCAGGCCCAG ATCCGAAAAG TGGACCAGGG TGGGTTAGGT
    GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CACAGTGCTT CTTTACGTAG CACCGGGAGC
    ATGTCAGTGG TGTCCGCAGC CCTGGTGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCT GTTCCCAAAG TTAGAGCAAT CCAGAGACAA
    CAGACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT
    CTAGATGAAG CCAAGCATGC TGAGAAGTAT GAACAAGAAA TTACTAAACT GAAGGAGCGC
    CTGAGAGTTT CCAGCCGGCG ACTGGAGGAA TATGAACGCC GCTTGCTGGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCCGACTGG AGGACAGCGA GGAGCGGCTC
    CGAAGACAGC AGGAAGAAAA AGATAGCCAG ATGAAAAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAG
    AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCAGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14676
    Clone ID Related Accession (Same CDS sequence) NM_170692.2 , NM_170692.3
    Accession Version NM_170692.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK311017.1, AL160281.17, BC110611.2, AL035702.7 and AA127237.1. On Jun 2, 2019 this sequence version replaced NM_170692.2. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.158295.1, BC110611.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GGCCTCTCCC TCGTGTTCTT GCTGATATTA CCAAGTCATT GACTAATCCT
    ACGCCAATAC AACAGCAACT GAGACGCTTC ACTGAACATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT
    AAACTAAAAT CTCCAAGCCA GGACAACACA GACAGCTACT TCAGAGGGAA AACATTATTG
    CTGGTTCAGC AAGCCTCCTC TCAGAGCATG ACTTATTCTG AAAAGGATGA AAGGGAAAGT
    AGCCTTCCTA ATGGTCGGAG CGTCTCCCTC ATGGACCTCC AGGACACTCA TGCTGCTCAA
    GTGGAGCATG CATCTGTCAT GCTTGATGTG CCTATACGCT TGACCGGAAG CCAGCTTTCC
    ATAACCCAGG TGGCCAGCAT CAAACAGCTG CGGGAAACCC AGAGCACTCC CCAAAGTGCA
    CCCCAAGTGA GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGTGGCCT TCAGCCCTTG
    TCGTTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CAGCAATGCC AAAGGCCTCT
    ATAGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA
    GACTTCAAGC TCAGTGGACC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACT
    CAGAGTGAGG ACTTCTCCAG GCGGCACACG GTGCCAGATA GACACATACC TCTTGCTTTG
    CCACGACAAA ATAGTACTGG GCAGGCCCAG ATCCGAAAAG TGGACCAGGG TGGGTTAGGT
    GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CACAGTGCTT CTTTACGTAG CACCGGGAGC
    ATGTCAGTGG TGTCCGCAGC CCTGGTGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCT GTTCCCAAAG TTAGAGCAAT CCAGAGACAA
    CAGACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT
    CTAGATGAAG CCAAGCATGC TGAGAAGTAT GAACAAGAAA TTACTAAACT GAAGGAGCGC
    CTGAGAGTTT CCAGCCGGCG ACTGGAGGAA TATGAACGCC GCTTGCTGGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCCGACTGG AGGACAGCGA GGAGCGGCTC
    CGAAGACAGC AGGAAGAAAA AGATAGCCAG ATGAAAAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAG
    AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCAGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733793.2
    CDS390..4232
    Translation

    Target ORF information:

    RefSeq Version NM_170692.3
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170692.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCGACCGTG
    GCAAAATTGG GGCCTCTCCC TCGTGTTCTT GCTGATATTA CCAAGTCATT GACTAATCCT
    ACGCCAATAC AACAGCAACT GAGACGCTTC ACTGAACATA ACTCCAGTCC AAATGTCAGT
    GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CCACTGACAG TGATTTGCAT
    AAACTAAAAT CTCCAAGCCA GGACAACACA GACAGCTACT TCAGAGGGAA AACATTATTG
    CTGGTTCAGC AAGCCTCCTC TCAGAGCATG ACTTATTCTG AAAAGGATGA AAGGGAAAGT
    AGCCTTCCTA ATGGTCGGAG CGTCTCCCTC ATGGACCTCC AGGACACTCA TGCTGCTCAA
    GTGGAGCATG CATCTGTCAT GCTTGATGTG CCTATACGCT TGACCGGAAG CCAGCTTTCC
    ATAACCCAGG TGGCCAGCAT CAAACAGCTG CGGGAAACCC AGAGCACTCC CCAAAGTGCA
    CCCCAAGTGA GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGTGGCCT TCAGCCCTTG
    TCGTTCCAGA ACCCTGTCTA TCACCTCAAT AACCCAATTC CAGCAATGCC AAAGGCCTCT
    ATAGATTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GAAGCCAAAG TAACAGTGAA
    GACTTCAAGC TCAGTGGACC CAGCAATAGC AGCATGGAAG ATTTCACTAA ACGTAGCACT
    CAGAGTGAGG ACTTCTCCAG GCGGCACACG GTGCCAGATA GACACATACC TCTTGCTTTG
    CCACGACAAA ATAGTACTGG GCAGGCCCAG ATCCGAAAAG TGGACCAGGG TGGGTTAGGT
    GCCCGAGCCA AAGCCCCACC ATCCCTGCCA CACAGTGCTT CTTTACGTAG CACCGGGAGC
    ATGTCAGTGG TGTCCGCAGC CCTGGTGGCC GAACCTGTGC AGAATGGGAG CCGGTCCCGG
    CAGCAGTCCT CTTCCTCCAG AGAGAGCCCT GTTCCCAAAG TTAGAGCAAT CCAGAGACAA
    CAGACACAGC AGGTTCAGTC ACCTGTGGAC TCTGCCACAA TGTCCCCAGT AGAGAGGACA
    GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGATG TGGAAGAAAC TGAGCAAAAT
    CTAGATGAAG CCAAGCATGC TGAGAAGTAT GAACAAGAAA TTACTAAACT GAAGGAGCGC
    CTGAGAGTTT CCAGCCGGCG ACTGGAGGAA TATGAACGCC GCTTGCTGGT GCAGGAGCAG
    CAGATGCAGA AGCTGCTGCT GGAATACAAG GCCCGACTGG AGGACAGCGA GGAGCGGCTC
    CGAAGACAGC AGGAAGAAAA AGATAGCCAG ATGAAAAGCA TCATCAGCAG GCTAATGGCT
    GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAAG CAGTTATTGA TGCAAAGCAG
    AAAATAATTG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCAGCCAA CACCAGACTG
    ATGAGCGCGC TGACCCAAGT GAAGGAGCGG TACAGCATGC AGGTCCGCAA TGGCATCTCC
    CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90810
    Clone ID Related Accession (Same CDS sequence) XM_017002849.1
    Accession Version XM_017002849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGG TGAAAATTCC TTCCTACAGG CGACCGTGGC AAAATTGGGG
    CCTCTCCCTC GTGTTCTTGC TGATATTACC AAGTCATTGA CTAATCCTAC GCCAATACAA
    CAGCAACTGA GACGCTTCAC TGAACATAAC TCCAGTCCAA ATGTCAGTGG AAGCCTCTCC
    TCTGGGCTGC AGAAAATATT TGAAGACCCC ACTGACAGTG ATTTGCATAA ACTAAAATCT
    CCAAGCCAGG ACAACACAGA CAGCTACTTC AGAGGGAAAA CATTATTGCT GGTTCAGCAA
    GCCTCCTCTC AGAGCATGAC TTATTCTGAA AAGGATGAAA GGGAAAGTAG CCTTCCTAAT
    GGTCGGAGCG TCTCCCTCAT GGACCTCCAG GACACTCATG CTGCTCAAGT GGAGCATGCA
    TCTGTCATGC TTGATGTGCC TATACGCTTG ACCGGAAGCC AGCTTTCCAT AACCCAGGTG
    GCCAGCATCA AACAGCTGCG GGAAACCCAG AGCACTCCCC AAAGTGCACC CCAAGTGAGA
    AGGCCCCTGC ACCCAGCCTT GAACCAGCCA GGTGGCCTTC AGCCCTTGTC GTTCCAGAAC
    CCTGTCTATC ACCTCAATAA CCCAATTCCA GCAATGCCAA AGGCCTCTAT AGATTCCAGT
    TTGGAGAACC TAAGCACTGC CAGTTCCAGA AGCCAAAGTA ACAGTGAAGA CTTCAAGCTC
    AGTGGACCCA GCAATAGCAG CATGGAAGAT TTCACTAAAC GTAGCACTCA GAGTGAGGAC
    TTCTCCAGGC GGCACACGGT GCCAGATAGA CACATACCTC TTGCTTTGCC ACGACAAAAT
    AGTACTGGGC AGGCCCAGAT CCGAAAAGTG GACCAGGGTG GGTTAGGTGC CCGAGCCAAA
    GCCCCACCAT CCCTGCCACA CAGTGCTTCT TTACGTAGCA CCGGGAGCAT GTCAGTGGTG
    TCCGCAGCCC TGGTGGCCGA ACCTGTGCAG AATGGGAGCC GGTCCCGGCA GCAGTCCTCT
    TCCTCCAGAG AGAGCCCTGT TCCCAAAGTT AGAGCAATCC AGAGACAACA GACACAGCAG
    GTTCAGTCAC CTGTGGACTC TGCCACAATG TCCCCAGTAG AGAGGACAGC AGCCTGGGTT
    CTGAACAATG GGCAGTATGA AGAGGATGTG GAAGAAACTG AGCAAAATCT AGATGAAGCC
    AAGCATGCTG AGAAGTATGA ACAAGAAATT ACTAAACTGA AGGAGCGCCT GAGAGTTTCC
    AGCCGGCGAC TGGAGGAATA TGAACGCCGC TTGCTGGTGC AGGAGCAGCA GATGCAGAAG
    CTGCTGCTGG AATACAAGGC CCGACTGGAG GACAGCGAGG AGCGGCTCCG AAGACAGCAG
    GAAGAAAAAG ATAGCCAGAT GAAAAGCATC ATCAGCAGGC TAATGGCTGT GGAAGAGGAA
    CTGAAGAAGG ATCATGCTGA GATGCAAGCA GTTATTGATG CAAAGCAGAA AATAATTGAT
    GCACAGGTCA TAAATGGAGA TGAGATTATT CAAACAGGAA AAACGGATCG TGTCCCTGGA
    TTCAGCCAAC ACCAGACTGA TGAGCGCGCT GACCCAAGTG AAGGAGCGGT ACAGCATGCA
    GGTCCGCAAT GGCATCTCCC CCACCAACCC CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858338.1
    CDS2984..7243
    Translation

    Target ORF information:

    RefSeq Version XM_017002849.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGG TGAAAATTCC TTCCTACAGG CGACCGTGGC AAAATTGGGG
    CCTCTCCCTC GTGTTCTTGC TGATATTACC AAGTCATTGA CTAATCCTAC GCCAATACAA
    CAGCAACTGA GACGCTTCAC TGAACATAAC TCCAGTCCAA ATGTCAGTGG AAGCCTCTCC
    TCTGGGCTGC AGAAAATATT TGAAGACCCC ACTGACAGTG ATTTGCATAA ACTAAAATCT
    CCAAGCCAGG ACAACACAGA CAGCTACTTC AGAGGGAAAA CATTATTGCT GGTTCAGCAA
    GCCTCCTCTC AGAGCATGAC TTATTCTGAA AAGGATGAAA GGGAAAGTAG CCTTCCTAAT
    GGTCGGAGCG TCTCCCTCAT GGACCTCCAG GACACTCATG CTGCTCAAGT GGAGCATGCA
    TCTGTCATGC TTGATGTGCC TATACGCTTG ACCGGAAGCC AGCTTTCCAT AACCCAGGTG
    GCCAGCATCA AACAGCTGCG GGAAACCCAG AGCACTCCCC AAAGTGCACC CCAAGTGAGA
    AGGCCCCTGC ACCCAGCCTT GAACCAGCCA GGTGGCCTTC AGCCCTTGTC GTTCCAGAAC
    CCTGTCTATC ACCTCAATAA CCCAATTCCA GCAATGCCAA AGGCCTCTAT AGATTCCAGT
    TTGGAGAACC TAAGCACTGC CAGTTCCAGA AGCCAAAGTA ACAGTGAAGA CTTCAAGCTC
    AGTGGACCCA GCAATAGCAG CATGGAAGAT TTCACTAAAC GTAGCACTCA GAGTGAGGAC
    TTCTCCAGGC GGCACACGGT GCCAGATAGA CACATACCTC TTGCTTTGCC ACGACAAAAT
    AGTACTGGGC AGGCCCAGAT CCGAAAAGTG GACCAGGGTG GGTTAGGTGC CCGAGCCAAA
    GCCCCACCAT CCCTGCCACA CAGTGCTTCT TTACGTAGCA CCGGGAGCAT GTCAGTGGTG
    TCCGCAGCCC TGGTGGCCGA ACCTGTGCAG AATGGGAGCC GGTCCCGGCA GCAGTCCTCT
    TCCTCCAGAG AGAGCCCTGT TCCCAAAGTT AGAGCAATCC AGAGACAACA GACACAGCAG
    GTTCAGTCAC CTGTGGACTC TGCCACAATG TCCCCAGTAG AGAGGACAGC AGCCTGGGTT
    CTGAACAATG GGCAGTATGA AGAGGATGTG GAAGAAACTG AGCAAAATCT AGATGAAGCC
    AAGCATGCTG AGAAGTATGA ACAAGAAATT ACTAAACTGA AGGAGCGCCT GAGAGTTTCC
    AGCCGGCGAC TGGAGGAATA TGAACGCCGC TTGCTGGTGC AGGAGCAGCA GATGCAGAAG
    CTGCTGCTGG AATACAAGGC CCGACTGGAG GACAGCGAGG AGCGGCTCCG AAGACAGCAG
    GAAGAAAAAG ATAGCCAGAT GAAAAGCATC ATCAGCAGGC TAATGGCTGT GGAAGAGGAA
    CTGAAGAAGG ATCATGCTGA GATGCAAGCA GTTATTGATG CAAAGCAGAA AATAATTGAT
    GCACAGGTCA TAAATGGAGA TGAGATTATT CAAACAGGAA AAACGGATCG TGTCCCTGGA
    TTCAGCCAAC ACCAGACTGA TGAGCGCGCT GACCCAAGTG AAGGAGCGGT ACAGCATGCA
    GGTCCGCAAT GGCATCTCCC CCACCAACCC CACCAAGCTT TCCATCACGG AGAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90811
    Clone ID Related Accession (Same CDS sequence) XM_017002850.1
    Accession Version XM_017002850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGG TGAAAATTCC TTCCTACAGG CGACCGTGGC AAAATTGGGG
    CCTCTCCCTC GTGTTCTTGC TGATATTACC AAGTCATTGA CTAATCCTAC GCCAATACAA
    CAGCAACTGA GACGCTTCAC TGAACATAAC TCCAGTCCAA ATGTCAGTGG AAGCCTCTCC
    TCTGGGCTGC AGAAAATATT TGAAGACCCC ACTGACAGTG ATTTGCATAA ACTAAAATCT
    CCAAGCCAGG ACAACACAGA CAGCTACTTC AGAGGGAAAA CATTATTGCT GGTTCAGCAA
    GCCTCCTCTC AGAGCATGAC TTATTCTGAA AAGGATGAAA GGGAAAGTAG CCTTCCTAAT
    GGTCGGAGCG TCTCCCTCAT GGACCTCCAG GACACTCATG CTGCTCAAGT GGAGCATGCA
    TCTGTCATGC TTGATGTGCC TATACGCTTG ACCGGAAGCC AGCTTTCCAT AACCCAGGTG
    GCCAGCATCA AACAGCTGCG GGAAACCCAG AGCACTCCCC AAAGTGCACC CCAAGTGAGA
    AGGCCCCTGC ACCCAGCCTT GAACCAGCCA GGTGGCCTTC AGCCCTTGTC GTTCCAGAAC
    CCTGTCTATC ACCTCAATAA CCCAATTCCA GCAATGCCAA AGGCCTCTAT AGATTCCAGT
    TTGGAGAACC TAAGCACTGC CAGTTCCAGA AGCCAAAGTA ACAGTGAAGA CTTCAAGCTC
    AGTGGACCCA GCAATAGCAG CATGGAAGAT TTCACTAAAC GTAGCACTCA GAGTGAGGAC
    TTCTCCAGGC GGCACACGGT GCCAGATAGA CACATACCTC TTGCTTTGCC ACGACAAAAT
    AGTACTGGGC AGGCCCAGAT CCGAAAAGTG GACCAGGGTG GGTTAGGTGC CCGAGCCAAA
    GCCCCACCAT CCCTGCCACA CAGTGCTTCT TTACGTAGCA CCGGGAGCAT GTCAGTGGTG
    TCCGCAGCCC TGGTGGCCGA ACCTGTGCAG AATGGGAGCC GGTCCCGGCA GCAGTCCTCT
    TCCTCCAGAG AGAGCCCTGT TCCCAAAGTT AGAGCAATCC AGAGACAACA GACACAGCAG
    GTTCAGTCAC CTGTGGACTC TGCCACAATG TCCCCAGTAG AGAGGACAGC AGCCTGGGTT
    CTGAACAATG GGCAGTATGA AGAGGATGTG GAAGAAACTG AGCAAAATCT AGATGAAGCC
    AAGCATGCTG AGAAGTATGA ACAAGAAATT ACTAAACTGA AGGAGCGCCT GAGAGTTTCC
    AGCCGGCGAC TGGAGGAATA TGAACGCCGC TTGCTGGTGC AGGAGCAGCA GATGCAGAAG
    CTGCTGCTGG AATACAAGGC CCGACTGGAG GACAGCGAGG AGCGGCTCCG AAGACAGCAG
    GAAGAAAAAG ATAGCCAGAT GAAAAGCATC ATCAGCAGGC TAATGGCTGT GGAAGAGGAA
    CTGAAGAAGG ATCATGCTGA GATGCAAGCA GTTATTGATG CAAAGCAGAA AATAATTGAT
    GCACAGGAAA AACGGATCGT GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG
    ACCCAAGTGA AGGAGCGGTA CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC
    ACCAAGCTTT CCATCACGGA GAATGGTGAA TTCAAAAACA GCAGCTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858339.1
    CDS2983..7233
    Translation

    Target ORF information:

    RefSeq Version XM_017002850.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGG TGAAAATTCC TTCCTACAGG CGACCGTGGC AAAATTGGGG
    CCTCTCCCTC GTGTTCTTGC TGATATTACC AAGTCATTGA CTAATCCTAC GCCAATACAA
    CAGCAACTGA GACGCTTCAC TGAACATAAC TCCAGTCCAA ATGTCAGTGG AAGCCTCTCC
    TCTGGGCTGC AGAAAATATT TGAAGACCCC ACTGACAGTG ATTTGCATAA ACTAAAATCT
    CCAAGCCAGG ACAACACAGA CAGCTACTTC AGAGGGAAAA CATTATTGCT GGTTCAGCAA
    GCCTCCTCTC AGAGCATGAC TTATTCTGAA AAGGATGAAA GGGAAAGTAG CCTTCCTAAT
    GGTCGGAGCG TCTCCCTCAT GGACCTCCAG GACACTCATG CTGCTCAAGT GGAGCATGCA
    TCTGTCATGC TTGATGTGCC TATACGCTTG ACCGGAAGCC AGCTTTCCAT AACCCAGGTG
    GCCAGCATCA AACAGCTGCG GGAAACCCAG AGCACTCCCC AAAGTGCACC CCAAGTGAGA
    AGGCCCCTGC ACCCAGCCTT GAACCAGCCA GGTGGCCTTC AGCCCTTGTC GTTCCAGAAC
    CCTGTCTATC ACCTCAATAA CCCAATTCCA GCAATGCCAA AGGCCTCTAT AGATTCCAGT
    TTGGAGAACC TAAGCACTGC CAGTTCCAGA AGCCAAAGTA ACAGTGAAGA CTTCAAGCTC
    AGTGGACCCA GCAATAGCAG CATGGAAGAT TTCACTAAAC GTAGCACTCA GAGTGAGGAC
    TTCTCCAGGC GGCACACGGT GCCAGATAGA CACATACCTC TTGCTTTGCC ACGACAAAAT
    AGTACTGGGC AGGCCCAGAT CCGAAAAGTG GACCAGGGTG GGTTAGGTGC CCGAGCCAAA
    GCCCCACCAT CCCTGCCACA CAGTGCTTCT TTACGTAGCA CCGGGAGCAT GTCAGTGGTG
    TCCGCAGCCC TGGTGGCCGA ACCTGTGCAG AATGGGAGCC GGTCCCGGCA GCAGTCCTCT
    TCCTCCAGAG AGAGCCCTGT TCCCAAAGTT AGAGCAATCC AGAGACAACA GACACAGCAG
    GTTCAGTCAC CTGTGGACTC TGCCACAATG TCCCCAGTAG AGAGGACAGC AGCCTGGGTT
    CTGAACAATG GGCAGTATGA AGAGGATGTG GAAGAAACTG AGCAAAATCT AGATGAAGCC
    AAGCATGCTG AGAAGTATGA ACAAGAAATT ACTAAACTGA AGGAGCGCCT GAGAGTTTCC
    AGCCGGCGAC TGGAGGAATA TGAACGCCGC TTGCTGGTGC AGGAGCAGCA GATGCAGAAG
    CTGCTGCTGG AATACAAGGC CCGACTGGAG GACAGCGAGG AGCGGCTCCG AAGACAGCAG
    GAAGAAAAAG ATAGCCAGAT GAAAAGCATC ATCAGCAGGC TAATGGCTGT GGAAGAGGAA
    CTGAAGAAGG ATCATGCTGA GATGCAAGCA GTTATTGATG CAAAGCAGAA AATAATTGAT
    GCACAGGAAA AACGGATCGT GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG
    ACCCAAGTGA AGGAGCGGTA CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC
    ACCAAGCTTT CCATCACGGA GAATGGTGAA TTCAAAAACA GCAGCTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90812
    Clone ID Related Accession (Same CDS sequence) XM_017002851.1
    Accession Version XM_017002851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4239bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGC GACCGTGGCA AAATTGGGGC CTCTCCCTCG TGTTCTTGCT
    GATATTACCA AGTCATTGAC TAATCCTACG CCAATACAAC AGCAACTGAG ACGCTTCACT
    GAACATAACT CCAGTCCAAA TGTCAGTGGA AGCCTCTCCT CTGGGCTGCA GAAAATATTT
    GAAGACCCCA CTGACAGTGA TTTGCATAAA CTAAAATCTC CAAGCCAGGA CAACACAGAC
    AGCTACTTCA GAGGGAAAAC ATTATTGCTG GTTCAGCAAG CCTCCTCTCA GAGCATGACT
    TATTCTGAAA AGGATGAAAG GGAAAGTAGC CTTCCTAATG GTCGGAGCGT CTCCCTCATG
    GACCTCCAGG ACACTCATGC TGCTCAAGTG GAGCATGCAT CTGTCATGCT TGATGTGCCT
    ATACGCTTGA CCGGAAGCCA GCTTTCCATA ACCCAGGTGG CCAGCATCAA ACAGCTGCGG
    GAAACCCAGA GCACTCCCCA AAGTGCACCC CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG
    AACCAGCCAG GTGGCCTTCA GCCCTTGTCG TTCCAGAACC CTGTCTATCA CCTCAATAAC
    CCAATTCCAG CAATGCCAAA GGCCTCTATA GATTCCAGTT TGGAGAACCT AAGCACTGCC
    AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC TTCAAGCTCA GTGGACCCAG CAATAGCAGC
    ATGGAAGATT TCACTAAACG TAGCACTCAG AGTGAGGACT TCTCCAGGCG GCACACGGTG
    CCAGATAGAC ACATACCTCT TGCTTTGCCA CGACAAAATA GTACTGGGCA GGCCCAGATC
    CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC CGAGCCAAAG CCCCACCATC CCTGCCACAC
    AGTGCTTCTT TACGTAGCAC CGGGAGCATG TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA
    CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT
    CCCAAAGTTA GAGCAATCCA GAGACAACAG ACACAGCAGG TTCAGTCACC TGTGGACTCT
    GCCACAATGT CCCCAGTAGA GAGGACAGCA GCCTGGGTTC TGAACAATGG GCAGTATGAA
    GAGGATGTGG AAGAAACTGA GCAAAATCTA GATGAAGCCA AGCATGCTGA GAAGTATGAA
    CAAGAAATTA CTAAACTGAA GGAGCGCCTG AGAGTTTCCA GCCGGCGACT GGAGGAATAT
    GAACGCCGCT TGCTGGTGCA GGAGCAGCAG ATGCAGAAGC TGCTGCTGGA ATACAAGGCC
    CGACTGGAGG ACAGCGAGGA GCGGCTCCGA AGACAGCAGG AAGAAAAAGA TAGCCAGATG
    AAAAGCATCA TCAGCAGGCT AATGGCTGTG GAAGAGGAAC TGAAGAAGGA TCATGCTGAG
    ATGCAAGCAG TTATTGATGC AAAGCAGAAA ATAATTGATG CACAGGTCAT AAATGGAGAT
    GAGATTATTC AAACAGGAAA AACGGATCGT GTCCCTGGAT TCAGCCAACA CCAGACTGAT
    GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA CAGCATGCAG GTCCGCAATG GCATCTCCCC
    CACCAACCCC ACCAAGCTTT CCATCACGGA GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858340.1
    CDS2983..7221
    Translation

    Target ORF information:

    RefSeq Version XM_017002851.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGC GACCGTGGCA AAATTGGGGC CTCTCCCTCG TGTTCTTGCT
    GATATTACCA AGTCATTGAC TAATCCTACG CCAATACAAC AGCAACTGAG ACGCTTCACT
    GAACATAACT CCAGTCCAAA TGTCAGTGGA AGCCTCTCCT CTGGGCTGCA GAAAATATTT
    GAAGACCCCA CTGACAGTGA TTTGCATAAA CTAAAATCTC CAAGCCAGGA CAACACAGAC
    AGCTACTTCA GAGGGAAAAC ATTATTGCTG GTTCAGCAAG CCTCCTCTCA GAGCATGACT
    TATTCTGAAA AGGATGAAAG GGAAAGTAGC CTTCCTAATG GTCGGAGCGT CTCCCTCATG
    GACCTCCAGG ACACTCATGC TGCTCAAGTG GAGCATGCAT CTGTCATGCT TGATGTGCCT
    ATACGCTTGA CCGGAAGCCA GCTTTCCATA ACCCAGGTGG CCAGCATCAA ACAGCTGCGG
    GAAACCCAGA GCACTCCCCA AAGTGCACCC CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG
    AACCAGCCAG GTGGCCTTCA GCCCTTGTCG TTCCAGAACC CTGTCTATCA CCTCAATAAC
    CCAATTCCAG CAATGCCAAA GGCCTCTATA GATTCCAGTT TGGAGAACCT AAGCACTGCC
    AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC TTCAAGCTCA GTGGACCCAG CAATAGCAGC
    ATGGAAGATT TCACTAAACG TAGCACTCAG AGTGAGGACT TCTCCAGGCG GCACACGGTG
    CCAGATAGAC ACATACCTCT TGCTTTGCCA CGACAAAATA GTACTGGGCA GGCCCAGATC
    CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC CGAGCCAAAG CCCCACCATC CCTGCCACAC
    AGTGCTTCTT TACGTAGCAC CGGGAGCATG TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA
    CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT
    CCCAAAGTTA GAGCAATCCA GAGACAACAG ACACAGCAGG TTCAGTCACC TGTGGACTCT
    GCCACAATGT CCCCAGTAGA GAGGACAGCA GCCTGGGTTC TGAACAATGG GCAGTATGAA
    GAGGATGTGG AAGAAACTGA GCAAAATCTA GATGAAGCCA AGCATGCTGA GAAGTATGAA
    CAAGAAATTA CTAAACTGAA GGAGCGCCTG AGAGTTTCCA GCCGGCGACT GGAGGAATAT
    GAACGCCGCT TGCTGGTGCA GGAGCAGCAG ATGCAGAAGC TGCTGCTGGA ATACAAGGCC
    CGACTGGAGG ACAGCGAGGA GCGGCTCCGA AGACAGCAGG AAGAAAAAGA TAGCCAGATG
    AAAAGCATCA TCAGCAGGCT AATGGCTGTG GAAGAGGAAC TGAAGAAGGA TCATGCTGAG
    ATGCAAGCAG TTATTGATGC AAAGCAGAAA ATAATTGATG CACAGGTCAT AAATGGAGAT
    GAGATTATTC AAACAGGAAA AACGGATCGT GTCCCTGGAT TCAGCCAACA CCAGACTGAT
    GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA CAGCATGCAG GTCCGCAATG GCATCTCCCC
    CACCAACCCC ACCAAGCTTT CCATCACGGA GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90813
    Clone ID Related Accession (Same CDS sequence) XM_017002852.1
    Accession Version XM_017002852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGC GACCGTGGCA AAATTGGGGC CTCTCCCTCG TGTTCTTGCT
    GATATTACCA AGTCATTGAC TAATCCTACG CCAATACAAC AGCAACTGAG ACGCTTCACT
    GAACATAACT CCAGTCCAAA TGTCAGTGGA AGCCTCTCCT CTGGGCTGCA GAAAATATTT
    GAAGACCCCA CTGACAGTGA TTTGCATAAA CTAAAATCTC CAAGCCAGGA CAACACAGAC
    AGCTACTTCA GAGGGAAAAC ATTATTGCTG GTTCAGCAAG CCTCCTCTCA GAGCATGACT
    TATTCTGAAA AGGATGAAAG GGAAAGTAGC CTTCCTAATG GTCGGAGCGT CTCCCTCATG
    GACCTCCAGG ACACTCATGC TGCTCAAGTG GAGCATGCAT CTGTCATGCT TGATGTGCCT
    ATACGCTTGA CCGGAAGCCA GCTTTCCATA ACCCAGGTGG CCAGCATCAA ACAGCTGCGG
    GAAACCCAGA GCACTCCCCA AAGTGCACCC CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG
    AACCAGCCAG GTGGCCTTCA GCCCTTGTCG TTCCAGAACC CTGTCTATCA CCTCAATAAC
    CCAATTCCAG CAATGCCAAA GGCCTCTATA GATTCCAGTT TGGAGAACCT AAGCACTGCC
    AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC TTCAAGCTCA GTGGACCCAG CAATAGCAGC
    ATGGAAGATT TCACTAAACG TAGCACTCAG AGTGAGGACT TCTCCAGGCG GCACACGGTG
    CCAGATAGAC ACATACCTCT TGCTTTGCCA CGACAAAATA GTACTGGGCA GGCCCAGATC
    CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC CGAGCCAAAG CCCCACCATC CCTGCCACAC
    AGTGCTTCTT TACGTAGCAC CGGGAGCATG TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA
    CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT
    CCCAAAGTTA GAGCAATCCA GAGACAACAG ACACAGCAGG TTCAGTCACC TGTGGACTCT
    GCCACAATGT CCCCAGTAGA GAGGACAGCA GCCTGGGTTC TGAACAATGG GCAGTATGAA
    GAGGATGTGG AAGAAACTGA GCAAAATCTA GATGAAGCCA AGCATGCTGA GAAGTATGAA
    CAAGAAATTA CTAAACTGAA GGAGCGCCTG AGAGTTTCCA GCCGGCGACT GGAGGAATAT
    GAACGCCGCT TGCTGGTGCA GGAGCAGCAG ATGCAGAAGC TGCTGCTGGA ATACAAGGCC
    CGACTGGAGG ACAGCGAGGA GCGGCTCCGA AGACAGCAGG AAGAAAAAGA TAGCCAGATG
    AAAAGCATCA TCAGCAGGCT AATGGCTGTG GAAGAGGAAC TGAAGAAGGA TCATGCTGAG
    ATGCAAGCAG TTATTGATGC AAAGCAGAAA ATAATTGATG CACAGGAAAA ACGGATCGTG
    TCCCTGGATT CAGCCAACAC CAGACTGATG AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC
    AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG
    AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858341.1
    CDS2982..7211
    Translation

    Target ORF information:

    RefSeq Version XM_017002852.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCATCT TTAAGAAACA AACATCACCT TGTCCTCCAA GCTATCGAAG ATCTTTGTCA 
    GAACCTGCTT ATGCAGATTC AGAGATAGGA ACAAGTTTAA AGAGTTATCC ATGGTGTGGT
    CCAGTGAAAA GCAAGAGGAA GAAAAATGAT TCTGCTGTTT CCAATCTGGG AAAGCCACTG
    GGTGCTTATC GGTGGCAGAG ACATCCGAGT TTTAGGTGGA AAGGGAACAA AAGGGAGAGA
    TTGCAGAGTA ATAAAGGAGA CCTGGTTGAA CGACAACAAC AAAAGGTCAT CCTGCCAAAG
    ATGGTTAGTA ATAATGCTTC TTCCTCTGCT GATGAGGTCC TTGGAACATC AACAGAGAAG
    CCACACCTGG TCAACAGCCG ATCACTTCGA GCACCATTCA CCAGATCTGT TTCAGAACCA
    GAACCCCGCT GCAGTATCCC ATTTGTTAGA CTGTGGCCAA CTTCACTTGG GTCAGTGGAG
    TCTGAGCAGC AGGGATATTT CATCCGTGGC ATTTTCTCTA CTCTTTCCAA TGGCTTCTCC
    AGGATGCTGA ATCTAAAAGG AGAATCAACC AATGAGCCAG TAAGAGAAGA TGTGAAAGGA
    CCACCCACCC ACCGTCTGTC TTGTGGTCAG TCACCCTACA CCGAGACAAC AACGTGGGAG
    CGGAAGTATT GCATCCTCAC AGACAGCCAG TTGGTATTGC TCAACAAGGA GAAGGAGATA
    CCTGTGGAAG GGGGACAGGA GCAGCAGACA GATTCCACCA AAGGGCGATG CCTGAGGAGA
    ACTGTCAGTG TCCCTTCCGA GGGTCAGTTT CCCGAGTACC CACCAGAGGG CGCCACTAAA
    CTGGAGGTAC CAGCAGAAAG GTCCCCTCGT AGACGGAGTA TCTCAGGGAC CAGTACATCA
    GAGAAACCCA ACTCCATGGA CACTGCAAAT ACCTCACCCT TCAAAGTACC AGGATTCTTC
    AGCAAGCGCC TGAAAGGCTC CATCAAGAGG ACCAAAAGCC AGTCAAAGCT TGACAGAAAC
    ACGAGCTTTC GGCTTCCATC CCTTCGCAGT ACAGATGACA GGTCTCGTGG GCTGCCTAAA
    CTAAAAGAGT CACGTTCCCA TGAATCCTTG CTGAGCCCAT GCAGCACAGT GGAATGTCTG
    GATCTTGGTA GAGGGGAACC TGTATCAGTG AAACCACTTC ATAGTAGCAT CCTTGGACAA
    GACTTCTGCT TTGAGGTTAC CTACTTAAGT GGAAGTAAAT GCTTCAGCTG TAATTCTGCT
    TCTGAGAGAG ACAAGTGGAT GGAAAACCTT CGCAGGACAG TTCAACCTAA TAAGGACAAT
    TGCAGGCGAG CTGAAAATGT TCTTCGTTTA TGGATCATTG AAGCCAAGGA CCTTGCCCCT
    AAAAAGAAAT ATTTCTGCGA ACTGTGCCTT GATGATACCC TCTTTGCTCG TACAACCAGC
    AAGACCAAAG CAGACAATAT TTTCTGGGGC GAACATTTTG AATTCTTCAG CCTTCCACCT
    CTTCATAGTA TCACAGTTCA CATTTACAAG GATGTGGAAA AAAAGAAAAA AAAGGACAAG
    AATAATTATG TAGGGCTAGT CAACATCCCC ACTGCCAGTG TGACTGGTCG CCAATTTGTA
    GAAAAGTGGT ATCCAGTGAG TACACCTACA CCCAACAAAG GAAAGACAGG AGGACCTTCT
    ATTCGGATTA AATCACGTTT CCAAACTATC ACCATTCTGC CTATGGAGCA ATACAAAGAA
    TTTGCAGAAT TTGTCACCAG CAACTACACC ATGCTGTGTT CTGTCCTTGA GCCAGTAATT
    AGTGTGAGAA ATAAAGAGGA GTTGGCTTGT GCCTTAGTGC ACATTCTTCA AAGTACTGGC
    AGAGCCAAGG ATTTTCTGAC TGACTTGGTG ATGTCTGAGG TGGATCGTTG TGGAGAGCAT
    GATGTCTTGA TCTTCAGAGA GAACACTATT GCCACCAAAT CCATTGAGGA ATACCTCAAG
    TTGGTGGGAC AACAGTATCT TCATGACGCA CTGGGGGAGT TTATCAAAGC TTTGTATGAG
    TCCGATGAGA ACTGTGAAGT GGATCCCAGC AAATGTTCAT CTAGTGAACT GATAGACCAT
    CAGAGCAACC TGAAAATGTG CTGTGAGCTG GCTTTCTGCA AGATCATCAA CTCTTACTGT
    GTTTTCCCTC GTGAGTTGAA AGAAGTGTTT GCATCATGGA AGCAGCAGTG CCTGAACCGT
    GGCAAGCAAG ACATCAGCGA GAGGCTCATC AGTGCCTCAT TATTTCTCCG TTTTCTGTGT
    CCAGCCATTA TGTCTCCCAG TCTTTTCAAC CTTATGCAGG AGTATCCTGA TGACCGCACA
    TCTCGGACTC TAACTCTTAT TGCCAAGGTC ATTCAGAACC TGGCCAACTT TGCCAAGTTT
    GGTAACAAAG AGGAATACAT GGCATTCATG AATGATTTTT TAGAACATGA ATGGGGTGGA
    ATGAAGCGCT TTCTTTTGGA GATCTCTAAT CCAGACACCA TCTCAAACAC CCCAGGCTTT
    GATGGTTACA TTGATCTGGG CCGAGAGCTT TCAGTTTTGC ATTCCTTACT GTGGGAAGTA
    GTTTCCCAAC TTGATAAGGC GACCGTGGCA AAATTGGGGC CTCTCCCTCG TGTTCTTGCT
    GATATTACCA AGTCATTGAC TAATCCTACG CCAATACAAC AGCAACTGAG ACGCTTCACT
    GAACATAACT CCAGTCCAAA TGTCAGTGGA AGCCTCTCCT CTGGGCTGCA GAAAATATTT
    GAAGACCCCA CTGACAGTGA TTTGCATAAA CTAAAATCTC CAAGCCAGGA CAACACAGAC
    AGCTACTTCA GAGGGAAAAC ATTATTGCTG GTTCAGCAAG CCTCCTCTCA GAGCATGACT
    TATTCTGAAA AGGATGAAAG GGAAAGTAGC CTTCCTAATG GTCGGAGCGT CTCCCTCATG
    GACCTCCAGG ACACTCATGC TGCTCAAGTG GAGCATGCAT CTGTCATGCT TGATGTGCCT
    ATACGCTTGA CCGGAAGCCA GCTTTCCATA ACCCAGGTGG CCAGCATCAA ACAGCTGCGG
    GAAACCCAGA GCACTCCCCA AAGTGCACCC CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG
    AACCAGCCAG GTGGCCTTCA GCCCTTGTCG TTCCAGAACC CTGTCTATCA CCTCAATAAC
    CCAATTCCAG CAATGCCAAA GGCCTCTATA GATTCCAGTT TGGAGAACCT AAGCACTGCC
    AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC TTCAAGCTCA GTGGACCCAG CAATAGCAGC
    ATGGAAGATT TCACTAAACG TAGCACTCAG AGTGAGGACT TCTCCAGGCG GCACACGGTG
    CCAGATAGAC ACATACCTCT TGCTTTGCCA CGACAAAATA GTACTGGGCA GGCCCAGATC
    CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC CGAGCCAAAG CCCCACCATC CCTGCCACAC
    AGTGCTTCTT TACGTAGCAC CGGGAGCATG TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA
    CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT
    CCCAAAGTTA GAGCAATCCA GAGACAACAG ACACAGCAGG TTCAGTCACC TGTGGACTCT
    GCCACAATGT CCCCAGTAGA GAGGACAGCA GCCTGGGTTC TGAACAATGG GCAGTATGAA
    GAGGATGTGG AAGAAACTGA GCAAAATCTA GATGAAGCCA AGCATGCTGA GAAGTATGAA
    CAAGAAATTA CTAAACTGAA GGAGCGCCTG AGAGTTTCCA GCCGGCGACT GGAGGAATAT
    GAACGCCGCT TGCTGGTGCA GGAGCAGCAG ATGCAGAAGC TGCTGCTGGA ATACAAGGCC
    CGACTGGAGG ACAGCGAGGA GCGGCTCCGA AGACAGCAGG AAGAAAAAGA TAGCCAGATG
    AAAAGCATCA TCAGCAGGCT AATGGCTGTG GAAGAGGAAC TGAAGAAGGA TCATGCTGAG
    ATGCAAGCAG TTATTGATGC AAAGCAGAAA ATAATTGATG CACAGGAAAA ACGGATCGTG
    TCCCTGGATT CAGCCAACAC CAGACTGATG AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC
    AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC CATCACGGAG
    AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90814
    Clone ID Related Accession (Same CDS sequence) XM_017002853.1
    Accession Version XM_017002853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGGAAA ATGCTTTACA TCTGTGGGAG AAAGATGTGA AAGGACCACC CACCCACCGT 
    CTGTCTTGTG GTCAGTCACC CTACACCGAG ACAACAACGT GGGAGCGGAA GTATTGCATC
    CTCACAGACA GCCAGTTGGT ATTGCTCAAC AAGGAGAAGG AGATACCTGT GGAAGGGGGA
    CAGGAGCAGC AGACAGATTC CACCAAAGGG CGATGCCTGA GGAGAACTGT CAGTGTCCCT
    TCCGAGGGTC AGTTTCCCGA GTACCCACCA GAGGGCGCCA CTAAACTGGA GGTACCAGCA
    GAAAGGTCCC CTCGTAGACG GAGTATCTCA GGGACCAGTA CATCAGAGAA ACCCAACTCC
    ATGGACACTG CAAATACCTC ACCCTTCAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA
    GGCTCCATCA AGAGGACCAA AAGCCAGTCA AAGCTTGACA GAAACACGAG CTTTCGGCTT
    CCATCCCTTC GCAGTACAGA TGACAGGTCT CGTGGGCTGC CTAAACTAAA AGAGTCACGT
    TCCCATGAAT CCTTGCTGAG CCCATGCAGC ACAGTGGAAT GTCTGGATCT TGGTAGAGGG
    GAACCTGTAT CAGTGAAACC ACTTCATAGT AGCATCCTTG GACAAGACTT CTGCTTTGAG
    GTTACCTACT TAAGTGGAAG TAAATGCTTC AGCTGTAATT CTGCTTCTGA GAGAGACAAG
    TGGATGGAAA ACCTTCGCAG GACAGTTCAA CCTAATAAGG ACAATTGCAG GCGAGCTGAA
    AATGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA GAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTCT GGGGCGAACA TTTTGAATTC TTCAGCCTTC CACCTCTTCA TAGTATCACA
    GTTCACATTT ACAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAACA TCCCCACTGC CAGTGTGACT GGTCGCCAAT TTGTAGAAAA GTGGTATCCA
    GTGAGTACAC CTACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAATCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC
    ACCAGCAACT ACACCATGCT GTGTTCTGTC CTTGAGCCAG TAATTAGTGT GAGAAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC TGAGGTGGAT CGTTGTGGAG AGCATGATGT CTTGATCTTC
    AGAGAGAACA CTATTGCCAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TATCTTCATG ACGCACTGGG GGAGTTTATC AAAGCTTTGT ATGAGTCCGA TGAGAACTGT
    GAAGTGGATC CCAGCAAATG TTCATCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGTGGCAA GCAAGACATC
    AGCGAGAGGC TCATCAGTGC CTCATTATTT CTCCGTTTTC TGTGTCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTTAT GCAGGAGTAT CCTGATGACC GCACATCTCG GACTCTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAA
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT
    TTGGAGATCT CTAATCCAGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT
    CTGGGCCGAG AGCTTTCAGT TTTGCATTCC TTACTGTGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGGCCTCT CCCTCGTGTT
    CTTGCTGATA TTACCAAGTC ATTGACTAAT CCTACGCCAA TACAACAGCA ACTGAGACGC
    TTCACTGAAC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA
    ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC
    ACAGACAGCT ACTTCAGAGG GAAAACATTA TTGCTGGTTC AGCAAGCCTC CTCTCAGAGC
    ATGACTTATT CTGAAAAGGA TGAAAGGGAA AGTAGCCTTC CTAATGGTCG GAGCGTCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAAGTGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATAC GCTTGACCGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAAAGT GCACCCCAAG TGAGAAGGCC CCTGCACCCA
    GCCTTGAACC AGCCAGGTGG CCTTCAGCCC TTGTCGTTCC AGAACCCTGT CTATCACCTC
    AATAACCCAA TTCCAGCAAT GCCAAAGGCC TCTATAGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGACTTCA AGCTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC TAAACGTAGC ACTCAGAGTG AGGACTTCTC CAGGCGGCAC
    ACGGTGCCAG ATAGACACAT ACCTCTTGCT TTGCCACGAC AAAATAGTAC TGGGCAGGCC
    CAGATCCGAA AAGTGGACCA GGGTGGGTTA GGTGCCCGAG CCAAAGCCCC ACCATCCCTG
    CCACACAGTG CTTCTTTACG TAGCACCGGG AGCATGTCAG TGGTGTCCGC AGCCCTGGTG
    GCCGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTTAGAGC AATCCAGAGA CAACAGACAC AGCAGGTTCA GTCACCTGTG
    GACTCTGCCA CAATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTTCTGAA CAATGGGCAG
    TATGAAGAGG ATGTGGAAGA AACTGAGCAA AATCTAGATG AAGCCAAGCA TGCTGAGAAG
    TATGAACAAG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TTTCCAGCCG GCGACTGGAG
    GAATATGAAC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC
    AAGGCCCGAC TGGAGGACAG CGAGGAGCGG CTCCGAAGAC AGCAGGAAGA AAAAGATAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAGATGAGA TTATTCAAAC AGGAAAAACG GATCGTGTCC CTGGATTCAG CCAACACCAG
    ACTGATGAGC GCGCTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858342.1
    CDS977..4681
    Translation

    Target ORF information:

    RefSeq Version XM_017002853.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATGGAAA ATGCTTTACA TCTGTGGGAG AAAGATGTGA AAGGACCACC CACCCACCGT 
    CTGTCTTGTG GTCAGTCACC CTACACCGAG ACAACAACGT GGGAGCGGAA GTATTGCATC
    CTCACAGACA GCCAGTTGGT ATTGCTCAAC AAGGAGAAGG AGATACCTGT GGAAGGGGGA
    CAGGAGCAGC AGACAGATTC CACCAAAGGG CGATGCCTGA GGAGAACTGT CAGTGTCCCT
    TCCGAGGGTC AGTTTCCCGA GTACCCACCA GAGGGCGCCA CTAAACTGGA GGTACCAGCA
    GAAAGGTCCC CTCGTAGACG GAGTATCTCA GGGACCAGTA CATCAGAGAA ACCCAACTCC
    ATGGACACTG CAAATACCTC ACCCTTCAAA GTACCAGGAT TCTTCAGCAA GCGCCTGAAA
    GGCTCCATCA AGAGGACCAA AAGCCAGTCA AAGCTTGACA GAAACACGAG CTTTCGGCTT
    CCATCCCTTC GCAGTACAGA TGACAGGTCT CGTGGGCTGC CTAAACTAAA AGAGTCACGT
    TCCCATGAAT CCTTGCTGAG CCCATGCAGC ACAGTGGAAT GTCTGGATCT TGGTAGAGGG
    GAACCTGTAT CAGTGAAACC ACTTCATAGT AGCATCCTTG GACAAGACTT CTGCTTTGAG
    GTTACCTACT TAAGTGGAAG TAAATGCTTC AGCTGTAATT CTGCTTCTGA GAGAGACAAG
    TGGATGGAAA ACCTTCGCAG GACAGTTCAA CCTAATAAGG ACAATTGCAG GCGAGCTGAA
    AATGTTCTTC GTTTATGGAT CATTGAAGCC AAGGACCTTG CCCCTAAAAA GAAATATTTC
    TGCGAACTGT GCCTTGATGA TACCCTCTTT GCTCGTACAA CCAGCAAGAC CAAAGCAGAC
    AATATTTTCT GGGGCGAACA TTTTGAATTC TTCAGCCTTC CACCTCTTCA TAGTATCACA
    GTTCACATTT ACAAGGATGT GGAAAAAAAG AAAAAAAAGG ACAAGAATAA TTATGTAGGG
    CTAGTCAACA TCCCCACTGC CAGTGTGACT GGTCGCCAAT TTGTAGAAAA GTGGTATCCA
    GTGAGTACAC CTACACCCAA CAAAGGAAAG ACAGGAGGAC CTTCTATTCG GATTAAATCA
    CGTTTCCAAA CTATCACCAT TCTGCCTATG GAGCAATACA AAGAATTTGC AGAATTTGTC
    ACCAGCAACT ACACCATGCT GTGTTCTGTC CTTGAGCCAG TAATTAGTGT GAGAAATAAA
    GAGGAGTTGG CTTGTGCCTT AGTGCACATT CTTCAAAGTA CTGGCAGAGC CAAGGATTTT
    CTGACTGACT TGGTGATGTC TGAGGTGGAT CGTTGTGGAG AGCATGATGT CTTGATCTTC
    AGAGAGAACA CTATTGCCAC CAAATCCATT GAGGAATACC TCAAGTTGGT GGGACAACAG
    TATCTTCATG ACGCACTGGG GGAGTTTATC AAAGCTTTGT ATGAGTCCGA TGAGAACTGT
    GAAGTGGATC CCAGCAAATG TTCATCTAGT GAACTGATAG ACCATCAGAG CAACCTGAAA
    ATGTGCTGTG AGCTGGCTTT CTGCAAGATC ATCAACTCTT ACTGTGTTTT CCCTCGTGAG
    TTGAAAGAAG TGTTTGCATC ATGGAAGCAG CAGTGCCTGA ACCGTGGCAA GCAAGACATC
    AGCGAGAGGC TCATCAGTGC CTCATTATTT CTCCGTTTTC TGTGTCCAGC CATTATGTCT
    CCCAGTCTTT TCAACCTTAT GCAGGAGTAT CCTGATGACC GCACATCTCG GACTCTAACT
    CTTATTGCCA AGGTCATTCA GAACCTGGCC AACTTTGCCA AGTTTGGTAA CAAAGAGGAA
    TACATGGCAT TCATGAATGA TTTTTTAGAA CATGAATGGG GTGGAATGAA GCGCTTTCTT
    TTGGAGATCT CTAATCCAGA CACCATCTCA AACACCCCAG GCTTTGATGG TTACATTGAT
    CTGGGCCGAG AGCTTTCAGT TTTGCATTCC TTACTGTGGG AAGTAGTTTC CCAACTTGAT
    AAGGGTGAAA ATTCCTTCCT ACAGGCGACC GTGGCAAAAT TGGGGCCTCT CCCTCGTGTT
    CTTGCTGATA TTACCAAGTC ATTGACTAAT CCTACGCCAA TACAACAGCA ACTGAGACGC
    TTCACTGAAC ATAACTCCAG TCCAAATGTC AGTGGAAGCC TCTCCTCTGG GCTGCAGAAA
    ATATTTGAAG ACCCCACTGA CAGTGATTTG CATAAACTAA AATCTCCAAG CCAGGACAAC
    ACAGACAGCT ACTTCAGAGG GAAAACATTA TTGCTGGTTC AGCAAGCCTC CTCTCAGAGC
    ATGACTTATT CTGAAAAGGA TGAAAGGGAA AGTAGCCTTC CTAATGGTCG GAGCGTCTCC
    CTCATGGACC TCCAGGACAC TCATGCTGCT CAAGTGGAGC ATGCATCTGT CATGCTTGAT
    GTGCCTATAC GCTTGACCGG AAGCCAGCTT TCCATAACCC AGGTGGCCAG CATCAAACAG
    CTGCGGGAAA CCCAGAGCAC TCCCCAAAGT GCACCCCAAG TGAGAAGGCC CCTGCACCCA
    GCCTTGAACC AGCCAGGTGG CCTTCAGCCC TTGTCGTTCC AGAACCCTGT CTATCACCTC
    AATAACCCAA TTCCAGCAAT GCCAAAGGCC TCTATAGATT CCAGTTTGGA GAACCTAAGC
    ACTGCCAGTT CCAGAAGCCA AAGTAACAGT GAAGACTTCA AGCTCAGTGG ACCCAGCAAT
    AGCAGCATGG AAGATTTCAC TAAACGTAGC ACTCAGAGTG AGGACTTCTC CAGGCGGCAC
    ACGGTGCCAG ATAGACACAT ACCTCTTGCT TTGCCACGAC AAAATAGTAC TGGGCAGGCC
    CAGATCCGAA AAGTGGACCA GGGTGGGTTA GGTGCCCGAG CCAAAGCCCC ACCATCCCTG
    CCACACAGTG CTTCTTTACG TAGCACCGGG AGCATGTCAG TGGTGTCCGC AGCCCTGGTG
    GCCGAACCTG TGCAGAATGG GAGCCGGTCC CGGCAGCAGT CCTCTTCCTC CAGAGAGAGC
    CCTGTTCCCA AAGTTAGAGC AATCCAGAGA CAACAGACAC AGCAGGTTCA GTCACCTGTG
    GACTCTGCCA CAATGTCCCC AGTAGAGAGG ACAGCAGCCT GGGTTCTGAA CAATGGGCAG
    TATGAAGAGG ATGTGGAAGA AACTGAGCAA AATCTAGATG AAGCCAAGCA TGCTGAGAAG
    TATGAACAAG AAATTACTAA ACTGAAGGAG CGCCTGAGAG TTTCCAGCCG GCGACTGGAG
    GAATATGAAC GCCGCTTGCT GGTGCAGGAG CAGCAGATGC AGAAGCTGCT GCTGGAATAC
    AAGGCCCGAC TGGAGGACAG CGAGGAGCGG CTCCGAAGAC AGCAGGAAGA AAAAGATAGC
    CAGATGAAAA GCATCATCAG CAGGCTAATG GCTGTGGAAG AGGAACTGAA GAAGGATCAT
    GCTGAGATGC AAGCAGTTAT TGATGCAAAG CAGAAAATAA TTGATGCACA GGTCATAAAT
    GGAGATGAGA TTATTCAAAC AGGAAAAACG GATCGTGTCC CTGGATTCAG CCAACACCAG
    ACTGATGAGC GCGCTGACCC AAGTGAAGGA GCGGTACAGC ATGCAGGTCC GCAATGGCAT
    CTCCCCCACC AACCCCACCA AGCTTTCCAT CACGGAGAAT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90815
    Clone ID Related Accession (Same CDS sequence) XM_017002854.1
    Accession Version XM_017002854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTACCA GCAGAAAGGT CCCCTCGTAG ACGGAGTATC 
    TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGCAAATAC CTCACCCTTC
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGCAGTAC AGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AATCCTTGCT GAGCCCATGC
    AGCACAGTGG AATGTCTGGA TCTTGGTAGA GGGGAACCTG TATCAGTGAA ACCACTTCAT
    AGTAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGCTGTA ATTCTGCTTC TGAGAGAGAC AAGTGGATGG AAAACCTTCG CAGGACAGTT
    CAACCTAATA AGGACAATTG CAGGCGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGCGA ACATTTTGAA
    TTCTTCAGCC TTCCACCTCT TCATAGTATC ACAGTTCACA TTTACAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT
    GTCCTTGAGC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACTATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCACT GGGGGAGTTT
    ATCAAAGCTT TGTATGAGTC CGATGAGAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCT
    AGTGAACTGA TAGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGTGG CAAGCAAGAC ATCAGCGAGA GGCTCATCAG TGCCTCATTA
    TTTCTCCGTT TTCTGTGTCC AGCCATTATG TCTCCCAGTC TTTTCAACCT TATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AATTGGGGCC TCTCCCTCGT GTTCTTGCTG ATATTACCAA GTCATTGACT
    AATCCTACGC CAATACAACA GCAACTGAGA CGCTTCACTG AACATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGACA GCTACTTCAG AGGGAAAACA
    TTATTGCTGG TTCAGCAAGC CTCCTCTCAG AGCATGACTT ATTCTGAAAA GGATGAAAGG
    GAAAGTAGCC TTCCTAATGG TCGGAGCGTC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAAGTGG AGCATGCATC TGTCATGCTT GATGTGCCTA TACGCTTGAC CGGAAGCCAG
    CTTTCCATAA CCCAGGTGGC CAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAA
    AGTGCACCCC AAGTGAGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG TGGCCTTCAG
    CCCTTGTCGT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATTCCAGC AATGCCAAAG
    GCCTCTATAG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC
    AGTGAAGACT TCAAGCTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT
    AGCACTCAGA GTGAGGACTT CTCCAGGCGG CACACGGTGC CAGATAGACA CATACCTCTT
    GCTTTGCCAC GACAAAATAG TACTGGGCAG GCCCAGATCC GAAAAGTGGA CCAGGGTGGG
    TTAGGTGCCC GAGCCAAAGC CCCACCATCC CTGCCACACA GTGCTTCTTT ACGTAGCACC
    GGGAGCATGT CAGTGGTGTC CGCAGCCCTG GTGGCCGAAC CTGTGCAGAA TGGGAGCCGG
    TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTTAG AGCAATCCAG
    AGACAACAGA CACAGCAGGT TCAGTCACCT GTGGACTCTG CCACAATGTC CCCAGTAGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGATGTGGA AGAAACTGAG
    CAAAATCTAG ATGAAGCCAA GCATGCTGAG AAGTATGAAC AAGAAATTAC TAAACTGAAG
    GAGCGCCTGA GAGTTTCCAG CCGGCGACTG GAGGAATATG AACGCCGCTT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCC GACTGGAGGA CAGCGAGGAG
    CGGCTCCGAA GACAGCAGGA AGAAAAAGAT AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA
    ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG AGCGCGCTGA CCCAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858343.1
    CDS776..4213
    Translation

    Target ORF information:

    RefSeq Version XM_017002854.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGAAGCA GTTGTGATAA AGAGGTACCA GCAGAAAGGT CCCCTCGTAG ACGGAGTATC 
    TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGCAAATAC CTCACCCTTC
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGCAGTAC AGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AATCCTTGCT GAGCCCATGC
    AGCACAGTGG AATGTCTGGA TCTTGGTAGA GGGGAACCTG TATCAGTGAA ACCACTTCAT
    AGTAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGCTGTA ATTCTGCTTC TGAGAGAGAC AAGTGGATGG AAAACCTTCG CAGGACAGTT
    CAACCTAATA AGGACAATTG CAGGCGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGCGA ACATTTTGAA
    TTCTTCAGCC TTCCACCTCT TCATAGTATC ACAGTTCACA TTTACAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT
    GTCCTTGAGC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACTATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCACT GGGGGAGTTT
    ATCAAAGCTT TGTATGAGTC CGATGAGAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCT
    AGTGAACTGA TAGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGTGG CAAGCAAGAC ATCAGCGAGA GGCTCATCAG TGCCTCATTA
    TTTCTCCGTT TTCTGTGTCC AGCCATTATG TCTCCCAGTC TTTTCAACCT TATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCG
    ACCGTGGCAA AATTGGGGCC TCTCCCTCGT GTTCTTGCTG ATATTACCAA GTCATTGACT
    AATCCTACGC CAATACAACA GCAACTGAGA CGCTTCACTG AACATAACTC CAGTCCAAAT
    GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCCAC TGACAGTGAT
    TTGCATAAAC TAAAATCTCC AAGCCAGGAC AACACAGACA GCTACTTCAG AGGGAAAACA
    TTATTGCTGG TTCAGCAAGC CTCCTCTCAG AGCATGACTT ATTCTGAAAA GGATGAAAGG
    GAAAGTAGCC TTCCTAATGG TCGGAGCGTC TCCCTCATGG ACCTCCAGGA CACTCATGCT
    GCTCAAGTGG AGCATGCATC TGTCATGCTT GATGTGCCTA TACGCTTGAC CGGAAGCCAG
    CTTTCCATAA CCCAGGTGGC CAGCATCAAA CAGCTGCGGG AAACCCAGAG CACTCCCCAA
    AGTGCACCCC AAGTGAGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG TGGCCTTCAG
    CCCTTGTCGT TCCAGAACCC TGTCTATCAC CTCAATAACC CAATTCCAGC AATGCCAAAG
    GCCTCTATAG ATTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGAAG CCAAAGTAAC
    AGTGAAGACT TCAAGCTCAG TGGACCCAGC AATAGCAGCA TGGAAGATTT CACTAAACGT
    AGCACTCAGA GTGAGGACTT CTCCAGGCGG CACACGGTGC CAGATAGACA CATACCTCTT
    GCTTTGCCAC GACAAAATAG TACTGGGCAG GCCCAGATCC GAAAAGTGGA CCAGGGTGGG
    TTAGGTGCCC GAGCCAAAGC CCCACCATCC CTGCCACACA GTGCTTCTTT ACGTAGCACC
    GGGAGCATGT CAGTGGTGTC CGCAGCCCTG GTGGCCGAAC CTGTGCAGAA TGGGAGCCGG
    TCCCGGCAGC AGTCCTCTTC CTCCAGAGAG AGCCCTGTTC CCAAAGTTAG AGCAATCCAG
    AGACAACAGA CACAGCAGGT TCAGTCACCT GTGGACTCTG CCACAATGTC CCCAGTAGAG
    AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGATGTGGA AGAAACTGAG
    CAAAATCTAG ATGAAGCCAA GCATGCTGAG AAGTATGAAC AAGAAATTAC TAAACTGAAG
    GAGCGCCTGA GAGTTTCCAG CCGGCGACTG GAGGAATATG AACGCCGCTT GCTGGTGCAG
    GAGCAGCAGA TGCAGAAGCT GCTGCTGGAA TACAAGGCCC GACTGGAGGA CAGCGAGGAG
    CGGCTCCGAA GACAGCAGGA AGAAAAAGAT AGCCAGATGA AAAGCATCAT CAGCAGGCTA
    ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAAGCAGT TATTGATGCA
    AAGCAGAAAA TAATTGATGC ACAGGTCATA AATGGAGATG AGATTATTCA AACAGGAAAA
    ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG AGCGCGCTGA CCCAAGTGAA
    GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC
    CATCACGGAG AATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90816
    Clone ID Related Accession (Same CDS sequence) XM_017002855.1
    Accession Version XM_017002855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGAAGCA GTTGTGATAA AGAGGTACCA GCAGAAAGGT CCCCTCGTAG ACGGAGTATC 
    TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGCAAATAC CTCACCCTTC
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGCAGTAC AGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AATCCTTGCT GAGCCCATGC
    AGCACAGTGG AATGTCTGGA TCTTGGTAGA GGGGAACCTG TATCAGTGAA ACCACTTCAT
    AGTAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGCTGTA ATTCTGCTTC TGAGAGAGAC AAGTGGATGG AAAACCTTCG CAGGACAGTT
    CAACCTAATA AGGACAATTG CAGGCGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGCGA ACATTTTGAA
    TTCTTCAGCC TTCCACCTCT TCATAGTATC ACAGTTCACA TTTACAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT
    GTCCTTGAGC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACTATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCACT GGGGGAGTTT
    ATCAAAGCTT TGTATGAGTC CGATGAGAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCT
    AGTGAACTGA TAGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGTGG CAAGCAAGAC ATCAGCGAGA GGCTCATCAG TGCCTCATTA
    TTTCTCCGTT TTCTGTGTCC AGCCATTATG TCTCCCAGTC TTTTCAACCT TATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGCGA CCGTGGCAAA ATTGGGGCCT
    CTCCCTCGTG TTCTTGCTGA TATTACCAAG TCATTGACTA ATCCTACGCC AATACAACAG
    CAACTGAGAC GCTTCACTGA ACATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT
    GGGCTGCAGA AAATATTTGA AGACCCCACT GACAGTGATT TGCATAAACT AAAATCTCCA
    AGCCAGGACA ACACAGACAG CTACTTCAGA GGGAAAACAT TATTGCTGGT TCAGCAAGCC
    TCCTCTCAGA GCATGACTTA TTCTGAAAAG GATGAAAGGG AAAGTAGCCT TCCTAATGGT
    CGGAGCGTCT CCCTCATGGA CCTCCAGGAC ACTCATGCTG CTCAAGTGGA GCATGCATCT
    GTCATGCTTG ATGTGCCTAT ACGCTTGACC GGAAGCCAGC TTTCCATAAC CCAGGTGGCC
    AGCATCAAAC AGCTGCGGGA AACCCAGAGC ACTCCCCAAA GTGCACCCCA AGTGAGAAGG
    CCCCTGCACC CAGCCTTGAA CCAGCCAGGT GGCCTTCAGC CCTTGTCGTT CCAGAACCCT
    GTCTATCACC TCAATAACCC AATTCCAGCA ATGCCAAAGG CCTCTATAGA TTCCAGTTTG
    GAGAACCTAA GCACTGCCAG TTCCAGAAGC CAAAGTAACA GTGAAGACTT CAAGCTCAGT
    GGACCCAGCA ATAGCAGCAT GGAAGATTTC ACTAAACGTA GCACTCAGAG TGAGGACTTC
    TCCAGGCGGC ACACGGTGCC AGATAGACAC ATACCTCTTG CTTTGCCACG ACAAAATAGT
    ACTGGGCAGG CCCAGATCCG AAAAGTGGAC CAGGGTGGGT TAGGTGCCCG AGCCAAAGCC
    CCACCATCCC TGCCACACAG TGCTTCTTTA CGTAGCACCG GGAGCATGTC AGTGGTGTCC
    GCAGCCCTGG TGGCCGAACC TGTGCAGAAT GGGAGCCGGT CCCGGCAGCA GTCCTCTTCC
    TCCAGAGAGA GCCCTGTTCC CAAAGTTAGA GCAATCCAGA GACAACAGAC ACAGCAGGTT
    CAGTCACCTG TGGACTCTGC CACAATGTCC CCAGTAGAGA GGACAGCAGC CTGGGTTCTG
    AACAATGGGC AGTATGAAGA GGATGTGGAA GAAACTGAGC AAAATCTAGA TGAAGCCAAG
    CATGCTGAGA AGTATGAACA AGAAATTACT AAACTGAAGG AGCGCCTGAG AGTTTCCAGC
    CGGCGACTGG AGGAATATGA ACGCCGCTTG CTGGTGCAGG AGCAGCAGAT GCAGAAGCTG
    CTGCTGGAAT ACAAGGCCCG ACTGGAGGAC AGCGAGGAGC GGCTCCGAAG ACAGCAGGAA
    GAAAAAGATA GCCAGATGAA AAGCATCATC AGCAGGCTAA TGGCTGTGGA AGAGGAACTG
    AAGAAGGATC ATGCTGAGAT GCAAGCAGTT ATTGATGCAA AGCAGAAAAT AATTGATGCA
    CAGGTCATAA ATGGAGATGA GATTATTCAA ACAGGAAAAA CGGATCGTGT CCCTGGATTC
    AGCCAACACC AGACTGATGA GCGCGCTGAC CCAAGTGAAG GAGCGGTACA GCATGCAGGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016858344.1
    CDS775..4191
    Translation

    Target ORF information:

    RefSeq Version XM_017002855.1
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGAAGCA GTTGTGATAA AGAGGTACCA GCAGAAAGGT CCCCTCGTAG ACGGAGTATC 
    TCAGGGACCA GTACATCAGA GAAACCCAAC TCCATGGACA CTGCAAATAC CTCACCCTTC
    AAAGTACCAG GATTCTTCAG CAAGCGCCTG AAAGGCTCCA TCAAGAGGAC CAAAAGCCAG
    TCAAAGCTTG ACAGAAACAC GAGCTTTCGG CTTCCATCCC TTCGCAGTAC AGATGACAGG
    TCTCGTGGGC TGCCTAAACT AAAAGAGTCA CGTTCCCATG AATCCTTGCT GAGCCCATGC
    AGCACAGTGG AATGTCTGGA TCTTGGTAGA GGGGAACCTG TATCAGTGAA ACCACTTCAT
    AGTAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC
    TTCAGCTGTA ATTCTGCTTC TGAGAGAGAC AAGTGGATGG AAAACCTTCG CAGGACAGTT
    CAACCTAATA AGGACAATTG CAGGCGAGCT GAAAATGTTC TTCGTTTATG GATCATTGAA
    GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGCGAAC TGTGCCTTGA TGATACCCTC
    TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATTT TCTGGGGCGA ACATTTTGAA
    TTCTTCAGCC TTCCACCTCT TCATAGTATC ACAGTTCACA TTTACAAGGA TGTGGAAAAA
    AAGAAAAAAA AGGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG
    ACTGGTCGCC AATTTGTAGA AAAGTGGTAT CCAGTGAGTA CACCTACACC CAACAAAGGA
    AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACTATCAC CATTCTGCCT
    ATGGAGCAAT ACAAAGAATT TGCAGAATTT GTCACCAGCA ACTACACCAT GCTGTGTTCT
    GTCCTTGAGC CAGTAATTAG TGTGAGAAAT AAAGAGGAGT TGGCTTGTGC CTTAGTGCAC
    ATTCTTCAAA GTACTGGCAG AGCCAAGGAT TTTCTGACTG ACTTGGTGAT GTCTGAGGTG
    GATCGTTGTG GAGAGCATGA TGTCTTGATC TTCAGAGAGA ACACTATTGC CACCAAATCC
    ATTGAGGAAT ACCTCAAGTT GGTGGGACAA CAGTATCTTC ATGACGCACT GGGGGAGTTT
    ATCAAAGCTT TGTATGAGTC CGATGAGAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCT
    AGTGAACTGA TAGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG
    ATCATCAACT CTTACTGTGT TTTCCCTCGT GAGTTGAAAG AAGTGTTTGC ATCATGGAAG
    CAGCAGTGCC TGAACCGTGG CAAGCAAGAC ATCAGCGAGA GGCTCATCAG TGCCTCATTA
    TTTCTCCGTT TTCTGTGTCC AGCCATTATG TCTCCCAGTC TTTTCAACCT TATGCAGGAG
    TATCCTGATG ACCGCACATC TCGGACTCTA ACTCTTATTG CCAAGGTCAT TCAGAACCTG
    GCCAACTTTG CCAAGTTTGG TAACAAAGAG GAATACATGG CATTCATGAA TGATTTTTTA
    GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACCATC
    TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT
    TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGCGA CCGTGGCAAA ATTGGGGCCT
    CTCCCTCGTG TTCTTGCTGA TATTACCAAG TCATTGACTA ATCCTACGCC AATACAACAG
    CAACTGAGAC GCTTCACTGA ACATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT
    GGGCTGCAGA AAATATTTGA AGACCCCACT GACAGTGATT TGCATAAACT AAAATCTCCA
    AGCCAGGACA ACACAGACAG CTACTTCAGA GGGAAAACAT TATTGCTGGT TCAGCAAGCC
    TCCTCTCAGA GCATGACTTA TTCTGAAAAG GATGAAAGGG AAAGTAGCCT TCCTAATGGT
    CGGAGCGTCT CCCTCATGGA CCTCCAGGAC ACTCATGCTG CTCAAGTGGA GCATGCATCT
    GTCATGCTTG ATGTGCCTAT ACGCTTGACC GGAAGCCAGC TTTCCATAAC CCAGGTGGCC
    AGCATCAAAC AGCTGCGGGA AACCCAGAGC ACTCCCCAAA GTGCACCCCA AGTGAGAAGG
    CCCCTGCACC CAGCCTTGAA CCAGCCAGGT GGCCTTCAGC CCTTGTCGTT CCAGAACCCT
    GTCTATCACC TCAATAACCC AATTCCAGCA ATGCCAAAGG CCTCTATAGA TTCCAGTTTG
    GAGAACCTAA GCACTGCCAG TTCCAGAAGC CAAAGTAACA GTGAAGACTT CAAGCTCAGT
    GGACCCAGCA ATAGCAGCAT GGAAGATTTC ACTAAACGTA GCACTCAGAG TGAGGACTTC
    TCCAGGCGGC ACACGGTGCC AGATAGACAC ATACCTCTTG CTTTGCCACG ACAAAATAGT
    ACTGGGCAGG CCCAGATCCG AAAAGTGGAC CAGGGTGGGT TAGGTGCCCG AGCCAAAGCC
    CCACCATCCC TGCCACACAG TGCTTCTTTA CGTAGCACCG GGAGCATGTC AGTGGTGTCC
    GCAGCCCTGG TGGCCGAACC TGTGCAGAAT GGGAGCCGGT CCCGGCAGCA GTCCTCTTCC
    TCCAGAGAGA GCCCTGTTCC CAAAGTTAGA GCAATCCAGA GACAACAGAC ACAGCAGGTT
    CAGTCACCTG TGGACTCTGC CACAATGTCC CCAGTAGAGA GGACAGCAGC CTGGGTTCTG
    AACAATGGGC AGTATGAAGA GGATGTGGAA GAAACTGAGC AAAATCTAGA TGAAGCCAAG
    CATGCTGAGA AGTATGAACA AGAAATTACT AAACTGAAGG AGCGCCTGAG AGTTTCCAGC
    CGGCGACTGG AGGAATATGA ACGCCGCTTG CTGGTGCAGG AGCAGCAGAT GCAGAAGCTG
    CTGCTGGAAT ACAAGGCCCG ACTGGAGGAC AGCGAGGAGC GGCTCCGAAG ACAGCAGGAA
    GAAAAAGATA GCCAGATGAA AAGCATCATC AGCAGGCTAA TGGCTGTGGA AGAGGAACTG
    AAGAAGGATC ATGCTGAGAT GCAAGCAGTT ATTGATGCAA AGCAGAAAAT AATTGATGCA
    CAGGTCATAA ATGGAGATGA GATTATTCAA ACAGGAAAAA CGGATCGTGT CCCTGGATTC
    AGCCAACACC AGACTGATGA GCGCGCTGAC CCAAGTGAAG GAGCGGTACA GCATGCAGGT
    CCGCAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14551
    Clone ID Related Accession (Same CDS sequence) NM_004841.3 , NM_004841.4
    Accession Version NM_004841.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF047711.1, AK290308.1, AL035702.7 and AA127237.1. On Apr 18, 2009 this sequence version replaced NM_004841.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF047711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004832.1
    CDS126..3545
    Translation

    Target ORF information:

    RefSeq Version NM_004841.3
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004841.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14551
    Clone ID Related Accession (Same CDS sequence) NM_004841.3 , NM_004841.4
    Accession Version NM_004841.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL365357.12, AF047711.1, AK290308.1, AL035702.7 and AA127237.1. On Jun 2, 2019 this sequence version replaced NM_004841.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF047711.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004832.1
    CDS146..3565
    Translation

    Target ORF information:

    RefSeq Version NM_004841.4
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004841.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CAGCCAACAC CAGACTGATG
    AGCGCGCTGA CCCAAGTGAA GGAGCGGTAC AGCATGCAGG TCCGCAATGG CATCTCCCCC
    ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34830
    Clone ID Related Accession (Same CDS sequence) XM_005245622.3 , XM_005245622.4
    Accession Version XM_005245622.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT
    GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245679.1
    CDS782..4210
    Translation

    Target ORF information:

    RefSeq Version XM_005245622.3
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245622.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT
    GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu34830
    Clone ID Related Accession (Same CDS sequence) XM_005245622.3 , XM_005245622.4
    Accession Version XM_005245622.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT
    GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005245679.1
    CDS68..3496
    Translation

    Target ORF information:

    RefSeq Version XM_005245622.4
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005245622.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGACCC CAGAGGTACC AGCAGAAAGG TCCCCTCGTA GACGGAGTAT CTCAGGGACC 
    AGTACATCAG AGAAACCCAA CTCCATGGAC ACTGCAAATA CCTCACCCTT CAAAGTACCA
    GGATTCTTCA GCAAGCGCCT GAAAGGCTCC ATCAAGAGGA CCAAAAGCCA GTCAAAGCTT
    GACAGAAACA CGAGCTTTCG GCTTCCATCC CTTCGCAGTA CAGATGACAG GTCTCGTGGG
    CTGCCTAAAC TAAAAGAGTC ACGTTCCCAT GAATCCTTGC TGAGCCCATG CAGCACAGTG
    GAATGTCTGG ATCTTGGTAG AGGGGAACCT GTATCAGTGA AACCACTTCA TAGTAGCATC
    CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGCTGT
    AATTCTGCTT CTGAGAGAGA CAAGTGGATG GAAAACCTTC GCAGGACAGT TCAACCTAAT
    AAGGACAATT GCAGGCGAGC TGAAAATGTT CTTCGTTTAT GGATCATTGA AGCCAAGGAC
    CTTGCCCCTA AAAAGAAATA TTTCTGCGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT
    ACAACCAGCA AGACCAAAGC AGACAATATT TTCTGGGGCG AACATTTTGA ATTCTTCAGC
    CTTCCACCTC TTCATAGTAT CACAGTTCAC ATTTACAAGG ATGTGGAAAA AAAGAAAAAA
    AAGGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC
    CAATTTGTAG AAAAGTGGTA TCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA
    GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACTATCA CCATTCTGCC TATGGAGCAA
    TACAAAGAAT TTGCAGAATT TGTCACCAGC AACTACACCA TGCTGTGTTC TGTCCTTGAG
    CCAGTAATTA GTGTGAGAAA TAAAGAGGAG TTGGCTTGTG CCTTAGTGCA CATTCTTCAA
    AGTACTGGCA GAGCCAAGGA TTTTCTGACT GACTTGGTGA TGTCTGAGGT GGATCGTTGT
    GGAGAGCATG ATGTCTTGAT CTTCAGAGAG AACACTATTG CCACCAAATC CATTGAGGAA
    TACCTCAAGT TGGTGGGACA ACAGTATCTT CATGACGCAC TGGGGGAGTT TATCAAAGCT
    TTGTATGAGT CCGATGAGAA CTGTGAAGTG GATCCCAGCA AATGTTCATC TAGTGAACTG
    ATAGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC
    TCTTACTGTG TTTTCCCTCG TGAGTTGAAA GAAGTGTTTG CATCATGGAA GCAGCAGTGC
    CTGAACCGTG GCAAGCAAGA CATCAGCGAG AGGCTCATCA GTGCCTCATT ATTTCTCCGT
    TTTCTGTGTC CAGCCATTAT GTCTCCCAGT CTTTTCAACC TTATGCAGGA GTATCCTGAT
    GACCGCACAT CTCGGACTCT AACTCTTATT GCCAAGGTCA TTCAGAACCT GGCCAACTTT
    GCCAAGTTTG GTAACAAAGA GGAATACATG GCATTCATGA ATGATTTTTT AGAACATGAA
    TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACCAT CTCAAACACC
    CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG
    TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC GACCGTGGCA
    AAATTGGGGC CTCTCCCTCG TGTTCTTGCT GATATTACCA AGTCATTGAC TAATCCTACG
    CCAATACAAC AGCAACTGAG ACGCTTCACT GAACATAACT CCAGTCCAAA TGTCAGTGGA
    AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCCA CTGACAGTGA TTTGCATAAA
    CTAAAATCTC CAAGCCAGGA CAACACAGAC AGCTACTTCA GAGGGAAAAC ATTATTGCTG
    GTTCAGCAAG CCTCCTCTCA GAGCATGACT TATTCTGAAA AGGATGAAAG GGAAAGTAGC
    CTTCCTAATG GTCGGAGCGT CTCCCTCATG GACCTCCAGG ACACTCATGC TGCTCAAGTG
    GAGCATGCAT CTGTCATGCT TGATGTGCCT ATACGCTTGA CCGGAAGCCA GCTTTCCATA
    ACCCAGGTGG CCAGCATCAA ACAGCTGCGG GAAACCCAGA GCACTCCCCA AAGTGCACCC
    CAAGTGAGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GTGGCCTTCA GCCCTTGTCG
    TTCCAGAACC CTGTCTATCA CCTCAATAAC CCAATTCCAG CAATGCCAAA GGCCTCTATA
    GATTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGAA GCCAAAGTAA CAGTGAAGAC
    TTCAAGCTCA GTGGACCCAG CAATAGCAGC ATGGAAGATT TCACTAAACG TAGCACTCAG
    AGTGAGGACT TCTCCAGGCG GCACACGGTG CCAGATAGAC ACATACCTCT TGCTTTGCCA
    CGACAAAATA GTACTGGGCA GGCCCAGATC CGAAAAGTGG ACCAGGGTGG GTTAGGTGCC
    CGAGCCAAAG CCCCACCATC CCTGCCACAC AGTGCTTCTT TACGTAGCAC CGGGAGCATG
    TCAGTGGTGT CCGCAGCCCT GGTGGCCGAA CCTGTGCAGA ATGGGAGCCG GTCCCGGCAG
    CAGTCCTCTT CCTCCAGAGA GAGCCCTGTT CCCAAAGTTA GAGCAATCCA GAGACAACAG
    ACACAGCAGG TTCAGTCACC TGTGGACTCT GCCACAATGT CCCCAGTAGA GAGGACAGCA
    GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGATGTGG AAGAAACTGA GCAAAATCTA
    GATGAAGCCA AGCATGCTGA GAAGTATGAA CAAGAAATTA CTAAACTGAA GGAGCGCCTG
    AGAGTTTCCA GCCGGCGACT GGAGGAATAT GAACGCCGCT TGCTGGTGCA GGAGCAGCAG
    ATGCAGAAGC TGCTGCTGGA ATACAAGGCC CGACTGGAGG ACAGCGAGGA GCGGCTCCGA
    AGACAGCAGG AAGAAAAAGA TAGCCAGATG AAAAGCATCA TCAGCAGGCT AATGGCTGTG
    GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAAGCAG TTATTGATGC AAAGCAGAAA
    ATAATTGATG CACAGGTCAT AAATGGAGAT GAGATTATTC AAACAGGAAA AACGGATCGT
    GTCCCTGGAT TCAGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAAGTGA AGGAGCGGTA
    CAGCATGCAG GTCCGCAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA
    GAATGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62841
    Clone ID Related Accession (Same CDS sequence) XM_011510166.2
    Accession Version XM_011510166.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGGCCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAGTCAT TGACTAATCC TACGCCAATA CAACAGCAAC TGAGACGCTT CACTGAACAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGACAGCTAC
    TTCAGAGGGA AAACATTATT GCTGGTTCAG CAAGCCTCCT CTCAGAGCAT GACTTATTCT
    GAAAAGGATG AAAGGGAAAG TAGCCTTCCT AATGGTCGGA GCGTCTCCCT CATGGACCTC
    CAGGACACTC ATGCTGCTCA AGTGGAGCAT GCATCTGTCA TGCTTGATGT GCCTATACGC
    TTGACCGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAGCT GCGGGAAACC
    CAGAGCACTC CCCAAAGTGC ACCCCAAGTG AGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGTGGCC TTCAGCCCTT GTCGTTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT
    CCAGCAATGC CAAAGGCCTC TATAGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGAAGCCAAA GTAACAGTGA AGACTTCAAG CTCAGTGGAC CCAGCAATAG CAGCATGGAA
    GATTTCACTA AACGTAGCAC TCAGAGTGAG GACTTCTCCA GGCGGCACAC GGTGCCAGAT
    AGACACATAC CTCTTGCTTT GCCACGACAA AATAGTACTG GGCAGGCCCA GATCCGAAAA
    GTGGACCAGG GTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACACAGTGCT
    TCTTTACGTA GCACCGGGAG CATGTCAGTG GTGTCCGCAG CCCTGGTGGC CGAACCTGTG
    CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC TGTTCCCAAA
    GTTAGAGCAA TCCAGAGACA ACAGACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA
    ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAT
    GTGGAAGAAA CTGAGCAAAA TCTAGATGAA GCCAAGCATG CTGAGAAGTA TGAACAAGAA
    ATTACTAAAC TGAAGGAGCG CCTGAGAGTT TCCAGCCGGC GACTGGAGGA ATATGAACGC
    CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCCGACTG
    GAGGACAGCG AGGAGCGGCT CCGAAGACAG CAGGAAGAAA AAGATAGCCA GATGAAAAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA
    GCAGTTATTG ATGCAAAGCA GAAAATAATT GATGCACAGG TCATAAATGG AGATGAGATT
    ATTCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCAGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508468.1
    CDS765..4637
    Translation

    Target ORF information:

    RefSeq Version XM_011510166.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510166.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGGCCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAGTCAT TGACTAATCC TACGCCAATA CAACAGCAAC TGAGACGCTT CACTGAACAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGACAGCTAC
    TTCAGAGGGA AAACATTATT GCTGGTTCAG CAAGCCTCCT CTCAGAGCAT GACTTATTCT
    GAAAAGGATG AAAGGGAAAG TAGCCTTCCT AATGGTCGGA GCGTCTCCCT CATGGACCTC
    CAGGACACTC ATGCTGCTCA AGTGGAGCAT GCATCTGTCA TGCTTGATGT GCCTATACGC
    TTGACCGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAGCT GCGGGAAACC
    CAGAGCACTC CCCAAAGTGC ACCCCAAGTG AGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGTGGCC TTCAGCCCTT GTCGTTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT
    CCAGCAATGC CAAAGGCCTC TATAGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGAAGCCAAA GTAACAGTGA AGACTTCAAG CTCAGTGGAC CCAGCAATAG CAGCATGGAA
    GATTTCACTA AACGTAGCAC TCAGAGTGAG GACTTCTCCA GGCGGCACAC GGTGCCAGAT
    AGACACATAC CTCTTGCTTT GCCACGACAA AATAGTACTG GGCAGGCCCA GATCCGAAAA
    GTGGACCAGG GTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACACAGTGCT
    TCTTTACGTA GCACCGGGAG CATGTCAGTG GTGTCCGCAG CCCTGGTGGC CGAACCTGTG
    CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC TGTTCCCAAA
    GTTAGAGCAA TCCAGAGACA ACAGACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA
    ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAT
    GTGGAAGAAA CTGAGCAAAA TCTAGATGAA GCCAAGCATG CTGAGAAGTA TGAACAAGAA
    ATTACTAAAC TGAAGGAGCG CCTGAGAGTT TCCAGCCGGC GACTGGAGGA ATATGAACGC
    CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCCGACTG
    GAGGACAGCG AGGAGCGGCT CCGAAGACAG CAGGAAGAAA AAGATAGCCA GATGAAAAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA
    GCAGTTATTG ATGCAAAGCA GAAAATAATT GATGCACAGG TCATAAATGG AGATGAGATT
    ATTCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCAGCC AACACCAGAC TGATGAGCGC
    GCTGACCCAA GTGAAGGAGC GGTACAGCAT GCAGGTCCGC AATGGCATCT CCCCCACCAA
    CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62842
    Clone ID Related Accession (Same CDS sequence) XM_011510167.2
    Accession Version XM_011510167.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ras GTPase-activating protein nGAP isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGGCCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAGTCAT TGACTAATCC TACGCCAATA CAACAGCAAC TGAGACGCTT CACTGAACAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGACAGCTAC
    TTCAGAGGGA AAACATTATT GCTGGTTCAG CAAGCCTCCT CTCAGAGCAT GACTTATTCT
    GAAAAGGATG AAAGGGAAAG TAGCCTTCCT AATGGTCGGA GCGTCTCCCT CATGGACCTC
    CAGGACACTC ATGCTGCTCA AGTGGAGCAT GCATCTGTCA TGCTTGATGT GCCTATACGC
    TTGACCGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAGCT GCGGGAAACC
    CAGAGCACTC CCCAAAGTGC ACCCCAAGTG AGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGTGGCC TTCAGCCCTT GTCGTTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT
    CCAGCAATGC CAAAGGCCTC TATAGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGAAGCCAAA GTAACAGTGA AGACTTCAAG CTCAGTGGAC CCAGCAATAG CAGCATGGAA
    GATTTCACTA AACGTAGCAC TCAGAGTGAG GACTTCTCCA GGCGGCACAC GGTGCCAGAT
    AGACACATAC CTCTTGCTTT GCCACGACAA AATAGTACTG GGCAGGCCCA GATCCGAAAA
    GTGGACCAGG GTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACACAGTGCT
    TCTTTACGTA GCACCGGGAG CATGTCAGTG GTGTCCGCAG CCCTGGTGGC CGAACCTGTG
    CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC TGTTCCCAAA
    GTTAGAGCAA TCCAGAGACA ACAGACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA
    ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAT
    GTGGAAGAAA CTGAGCAAAA TCTAGATGAA GCCAAGCATG CTGAGAAGTA TGAACAAGAA
    ATTACTAAAC TGAAGGAGCG CCTGAGAGTT TCCAGCCGGC GACTGGAGGA ATATGAACGC
    CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCCGACTG
    GAGGACAGCG AGGAGCGGCT CCGAAGACAG CAGGAAGAAA AAGATAGCCA GATGAAAAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA
    GCAGTTATTG ATGCAAAGCA GAAAATAATT GATGCACAGG AAAAACGGAT CGTGTCCCTG
    GATTCAGCCA ACACCAGACT GATGAGCGCG CTGACCCAAG TGAAGGAGCG GTACAGCATG
    CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508469.1
    CDS765..4628
    Translation

    Target ORF information:

    RefSeq Version XM_011510167.2
    Organism Homo sapiens(human)
    Definition Homo sapiens RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011510167.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAGCTCT CTCCGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC GGAGATGTTC 
    CCGGCGCTGG AGTCCGACTC GCCGCTGCCC CCGGAGGACC TGGACGCGGT TGTCCCAGTC
    AGTGGAGCCG TCGCCGGTGG CATGTTGGAT CGGATCCTTC TGGAGTCCGT GTGCCAGCAA
    CAGAGCTGGG TCCGGGTGTA CGATGTGAAA GGACCACCCA CCCACCGTCT GTCTTGTGGT
    CAGTCACCCT ACACCGAGAC AACAACGTGG GAGCGGAAGT ATTGCATCCT CACAGACAGC
    CAGTTGGTAT TGCTCAACAA GGAGAAGGAG ATACCTGTGG AAGGGGGACA GGAGCAGCAG
    ACAGATTCCA CCAAAGGGCG ATGCCTGAGG AGAACTGTCA GTGTCCCTTC CGAGGGTCAG
    TTTCCCGAGT ACCCACCAGA GGGCGCCACT AAACTGGAGG TACCAGCAGA AAGGTCCCCT
    CGTAGACGGA GTATCTCAGG GACCAGTACA TCAGAGAAAC CCAACTCCAT GGACACTGCA
    AATACCTCAC CCTTCAAAGT ACCAGGATTC TTCAGCAAGC GCCTGAAAGG CTCCATCAAG
    AGGACCAAAA GCCAGTCAAA GCTTGACAGA AACACGAGCT TTCGGCTTCC ATCCCTTCGC
    AGTACAGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCACGTTC CCATGAATCC
    TTGCTGAGCC CATGCAGCAC AGTGGAATGT CTGGATCTTG GTAGAGGGGA ACCTGTATCA
    GTGAAACCAC TTCATAGTAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA
    AGTGGAAGTA AATGCTTCAG CTGTAATTCT GCTTCTGAGA GAGACAAGTG GATGGAAAAC
    CTTCGCAGGA CAGTTCAACC TAATAAGGAC AATTGCAGGC GAGCTGAAAA TGTTCTTCGT
    TTATGGATCA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG CGAACTGTGC
    CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATTTTCTGG
    GGCGAACATT TTGAATTCTT CAGCCTTCCA CCTCTTCATA GTATCACAGT TCACATTTAC
    AAGGATGTGG AAAAAAAGAA AAAAAAGGAC AAGAATAATT ATGTAGGGCT AGTCAACATC
    CCCACTGCCA GTGTGACTGG TCGCCAATTT GTAGAAAAGT GGTATCCAGT GAGTACACCT
    ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACT
    ATCACCATTC TGCCTATGGA GCAATACAAA GAATTTGCAG AATTTGTCAC CAGCAACTAC
    ACCATGCTGT GTTCTGTCCT TGAGCCAGTA ATTAGTGTGA GAAATAAAGA GGAGTTGGCT
    TGTGCCTTAG TGCACATTCT TCAAAGTACT GGCAGAGCCA AGGATTTTCT GACTGACTTG
    GTGATGTCTG AGGTGGATCG TTGTGGAGAG CATGATGTCT TGATCTTCAG AGAGAACACT
    ATTGCCACCA AATCCATTGA GGAATACCTC AAGTTGGTGG GACAACAGTA TCTTCATGAC
    GCACTGGGGG AGTTTATCAA AGCTTTGTAT GAGTCCGATG AGAACTGTGA AGTGGATCCC
    AGCAAATGTT CATCTAGTGA ACTGATAGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG
    CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTCC CTCGTGAGTT GAAAGAAGTG
    TTTGCATCAT GGAAGCAGCA GTGCCTGAAC CGTGGCAAGC AAGACATCAG CGAGAGGCTC
    ATCAGTGCCT CATTATTTCT CCGTTTTCTG TGTCCAGCCA TTATGTCTCC CAGTCTTTTC
    AACCTTATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTAACTCT TATTGCCAAG
    GTCATTCAGA ACCTGGCCAA CTTTGCCAAG TTTGGTAACA AAGAGGAATA CATGGCATTC
    ATGAATGATT TTTTAGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT
    AATCCAGACA CCATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG
    CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT
    TCCTTCCTAC AGGCGACCGT GGCAAAATTG GGGCCTCTCC CTCGTGTTCT TGCTGATATT
    ACCAAGTCAT TGACTAATCC TACGCCAATA CAACAGCAAC TGAGACGCTT CACTGAACAT
    AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC
    CCCACTGACA GTGATTTGCA TAAACTAAAA TCTCCAAGCC AGGACAACAC AGACAGCTAC
    TTCAGAGGGA AAACATTATT GCTGGTTCAG CAAGCCTCCT CTCAGAGCAT GACTTATTCT
    GAAAAGGATG AAAGGGAAAG TAGCCTTCCT AATGGTCGGA GCGTCTCCCT CATGGACCTC
    CAGGACACTC ATGCTGCTCA AGTGGAGCAT GCATCTGTCA TGCTTGATGT GCCTATACGC
    TTGACCGGAA GCCAGCTTTC CATAACCCAG GTGGCCAGCA TCAAACAGCT GCGGGAAACC
    CAGAGCACTC CCCAAAGTGC ACCCCAAGTG AGAAGGCCCC TGCACCCAGC CTTGAACCAG
    CCAGGTGGCC TTCAGCCCTT GTCGTTCCAG AACCCTGTCT ATCACCTCAA TAACCCAATT
    CCAGCAATGC CAAAGGCCTC TATAGATTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC
    AGAAGCCAAA GTAACAGTGA AGACTTCAAG CTCAGTGGAC CCAGCAATAG CAGCATGGAA
    GATTTCACTA AACGTAGCAC TCAGAGTGAG GACTTCTCCA GGCGGCACAC GGTGCCAGAT
    AGACACATAC CTCTTGCTTT GCCACGACAA AATAGTACTG GGCAGGCCCA GATCCGAAAA
    GTGGACCAGG GTGGGTTAGG TGCCCGAGCC AAAGCCCCAC CATCCCTGCC ACACAGTGCT
    TCTTTACGTA GCACCGGGAG CATGTCAGTG GTGTCCGCAG CCCTGGTGGC CGAACCTGTG
    CAGAATGGGA GCCGGTCCCG GCAGCAGTCC TCTTCCTCCA GAGAGAGCCC TGTTCCCAAA
    GTTAGAGCAA TCCAGAGACA ACAGACACAG CAGGTTCAGT CACCTGTGGA CTCTGCCACA
    ATGTCCCCAG TAGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAT
    GTGGAAGAAA CTGAGCAAAA TCTAGATGAA GCCAAGCATG CTGAGAAGTA TGAACAAGAA
    ATTACTAAAC TGAAGGAGCG CCTGAGAGTT TCCAGCCGGC GACTGGAGGA ATATGAACGC
    CGCTTGCTGG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAATACAA GGCCCGACTG
    GAGGACAGCG AGGAGCGGCT CCGAAGACAG CAGGAAGAAA AAGATAGCCA GATGAAAAGC
    ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAA
    GCAGTTATTG ATGCAAAGCA GAAAATAATT GATGCACAGG AAAAACGGAT CGTGTCCCTG
    GATTCAGCCA ACACCAGACT GATGAGCGCG CTGACCCAAG TGAAGGAGCG GTACAGCATG
    CAGGTCCGCA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT
    GAATTCAAAA ACAGCAGCTG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.