SLIT2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001289135.2	NP_001276064.1	slit homolog 2 protein isoform 2 precursor
XM_017008845.1	XP_016864334.1	slit homolog 2 protein isoform X4
NM_004787.3	NP_004778.1	slit homolog 2 protein isoform 1 precursor
NM_004787.4	NP_004778.1	slit homolog 2 protein isoform 1 precursor
XM_006713986.3	XP_006714049.1	slit homolog 2 protein isoform X2
XM_011513910.2	XP_011512212.2	slit homolog 2 protein isoform X5
NM_004787.2	NP_004778.1	slit homolog 2 protein isoform 1 precursor
NM_001289136.2	NP_001276065.1	slit homolog 2 protein isoform 3 precursor
NM_001289136.3	NP_001276065.1	slit homolog 2 protein isoform 3 precursor
NM_001289135.3	NP_001276064.1	slit homolog 2 protein isoform 2 precursor
XM_005248211.3	XP_005248268.1	slit homolog 2 protein isoform X1
XM_011513909.2	XP_011512211.1	slit homolog 2 protein isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23098	NM_004787.4 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu95551	XM_017008845.1 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu95552	XM_011513910.2 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu22199	NM_001289135.3 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu22846	NM_001289136.3 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38497	XM_005248211.3 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu38498	XM_006713986.3 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu62720	XM_011513909.2 Latest version!	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu23098	NM_004787.3	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu23098	NM_004787.2	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu22846	NM_001289136.2	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu22199	NM_001289135.2	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

SLIT2 ( NM_004787.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004778.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23098
Clone ID Related Accession (Same CDS sequence)	NM_004787.3 , NM_004787.4 , NM_004787.2
Accession Version	NM_004787.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC117190.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. This sequence is a reference standard in the RefSeqGene project. On Sep 10, 2015 this sequence version replaced NM_004787.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB017168.1, BC117190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004778.1
CDS	1912..6501
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_004787.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004787.3

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_004787.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004778.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23098
Clone ID Related Accession (Same CDS sequence)	NM_004787.3 , NM_004787.4 , NM_004787.2
Accession Version	NM_004787.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC117190.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004787.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB017168.1, BC117190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000504154.6/ ENSP00000422591.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004778.1
CDS	1912..6501
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_004787.4
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004787.4

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_004787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004778.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

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CloneID	OHu23098
Clone ID Related Accession (Same CDS sequence)	NM_004787.3 , NM_004787.4 , NM_004787.2
Accession Version	NM_004787.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-28
Organism	Homo sapiens(Human)
Product	slit homolog 2 protein isoform 1 precursor
Comment

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_004778.1

Translation

MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_004787.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004787.2

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCGGATC TGGCTTGCCC TGAAAAGTGT CGCTGTGAAG GAACCACAGT AGATTGCTCT 
AATCAAAAGC TCAACAAAAT CCCGGAGCAC ATTCCCCAGT ACACTGCAGA GTTGCGTCTC 
AATAATAATG AATTTACCGT GTTGGAAGCC ACAGGAATCT TTAAGAAACT TCCTCAATTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGATATTG AGGAGGGAGC ATTTGAAGGA 
GCATCTGGTG TAAATGAAAT ACTTCTTACG AGTAATCGTT TGGAAAATGT GCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTCAAAACT TTGATGTTGA GAAGCAATCG AATAACCTGT 
GTGGGGAATG ACAGTTTCAT AGGACTCAGT TCTGTGCGTT TGCTTTCTTT GTATGATAAT 
CAAATTACTA CAGTTGCACC AGGGGCATTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTTAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA GTGGCTGAGA 
AAGAAGAGAA TTGTCACGGG AAATCCTAGA TGTCAAAAAC CATACTTCCT GAAAGAAATA 
CCCATCCAGG ATGTGGCCAT TCAGGACTTC ACTTGTGATG ACGGAAATGA TGACAATAGT 
TGCTCCCCAC TTTCTCGCTG TCCTACTGAA TGTACTTGCT TGGATACAGT CGTCCGATGT 
AGCAACAAGG GTTTGAAGGT CTTGCCGAAA GGTATTCCAA GAGATGTCAC AGAGTTGTAT 
CTGGATGGAA ACCAATTTAC ACTGGTTCCC AAGGAACTCT CCAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACGCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCTTAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_017008845.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864334.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

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CloneID	OHu95551
Clone ID Related Accession (Same CDS sequence)	XM_017008845.1
Accession Version	XM_017008845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG CAAGTGAGAC GCCACTTTGT TCTCCAGAGT CCATAAACGG AGTCACCTTG 
CGATTGCCAG CATCCAGGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT 
TTTGCTGGTC TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT 
GAAAGAGGAG CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC 
CTTCAGCTGT TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC 
AGTGAAAACC AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA 
AATTTGCAAC TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC 
CGGGACCTGG AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT 
TTCAACCATA TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC 
TGCCACCTGG CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT 
CAGTGTATGG GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA 
TTTGTCTGCA GTGGTCACCA GTCATTTATG GCTCCTTCTT GTAGTGTTTT GCACTGCCCT 
GCCGCCTGTA CCTGTAGCAA CAATATCGTA GACTGTCGTG GGAAAGGTCT CACTGAGATC 
CCCACAAATC TTCCAGAGAC CATCACAGAA ATACGTTTGG AACAGAACAC AATCAAAGTC 
ATCCCTCCTG GAGCTTTCTC ACCATATAAA AAGCTTAGAC GAATTGACCT GAGCAATAAT 
CAGATCTCTG AACTTGCACC AGATGCTTTC CAAGGACTAC GCTCTCTGAA TTCACTTGTC 
CTCTATGGAA ATAAAATCAC AGAACTCCCC AAAAGTTTAT TTGAAGGACT GTTTTCCTTA 
CAGCTCCTAT TATTGAATGC CAACAAGATA AACTGCCTTC GGGTAGATGC TTTTCAGGAT 
CTCCACAACT TGAACCTTCT CTCCCTATAT GACAACAAGC TTCAGACCAT CGCCAAGGGG 
ACCTTTTCAC CTCTTCGGGC CATTCAAACT ATGCATTTGG CCCAGAACCC CTTTATTTGT 
GACTGCCATC TCAAGTGGCT AGCGGATTAT CTCCATACCA ACCCGATTGA GACCAGTGGT 
GCCCGTTGCA CCAGCCCCCG CCGCCTGGCA AACAAAAGAA TTGGACAGAT CAAAAGCAAG 
AAATTCCGTT GTTCAGCTAA AGAACAGTAT TTCATTCCAG GTACAGAAGA TTATCGATCA 
AAATTAAGTG GAGACTGCTT TGCGGATCTG GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA 
ACCACAGTAG ATTGCTCTAA TCAAAAGCTC AACAAAATCC CGGAGCACAT TCCCCAGTAC 
ACTGCAGAGT TGCGTCTCAA TAATAATGAA TTTACCGTGT TGGAAGCCAC AGGAATCTTT 
AAGAAACTTC CTCAATTACG TAAAATAAAC TTTAGCAACA ATAAGATCAC AGATATTGAG 
GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA AATGAAATAC TTCTTACGAG TAATCGTTTG 
GAAAATGTGC AGCATAAGAT GTTCAAGGGA TTGGAAAGCC TCAAAACTTT GATGTTGAGA 
AGCAATCGAA TAACCTGTGT GGGGAATGAC AGTTTCATAG GACTCAGTTC TGTGCGTTTG 
CTTTCTTTGT ATGATAATCA AATTACTACA GTTGCACCAG GGGCATTTGA TACTCTCCAT 
TCTTTATCTA CTCTAAACCT CTTGGCCAAT CCTTTTAACT GTAACTGCTA CCTGGCTTGG 
TTGGGAGAGT GGCTGAGAAA GAAGAGAATT GTCACGGGAA ATCCTAGATG TCAAAAACCA 
TACTTCCTGA AAGAAATACC CATCCAGGAT GTGGCCATTC AGGACTTCAC TTGTGATGAC 
GGAAATGATG ACAATAGTTG CTCCCCACTT TCTCGCTGTC CTACTGAATG TACTTGCTTG 
GATACAGTCG TCCGATGTAG CAACAAGGGT TTGAAGGTCT TGCCGAAAGG TATTCCAAGA 
GATGTCACAG AGTTGTATCT GGATGGAAAC CAATTTACAC TGGTTCCCAA GGAACTCTCC 
AACTACAAAC ATTTAACACT TATAGACTTA AGTAACAACA GAATAAGCAC GCTTTCTAAT 
CAGAGCTTCA GCAACATGAC CCAGCTCCTC ACCTTAATTC TTAGTTACAA CCGTCTGAGA 
TGTATTCCTC CTCGCACCTT TGATGGATTA AAGTCTCTTC GATTACTTTC TCTACATGGA 
AATGACATTT CTGTTGTGCC TGAAGGTGCT TTCAATGATC TTTCTGCATT ATCACATCTA 
GCAATTGGAG CCAACCCTCT TTACTGTGAT TGTAACATGC AGTGGTTATC CGACTGGGTG 
AAGTCGGAAT ATAAGGAGCC TGGAATTGCT CGTTGTGCTG GTCCTGGAGA AATGGCAGAT 
AAACTTTTAC TCACAACTCC CTCCAAAAAA TTTACCTGTC AAGGTCCTGT GGATGTCAAT 
ATTCTAGCTA AGTGTAACCC CTGCCTATCA AATCCGTGTA AAAATGATGG CACATGTAAT 
AGTGATCCAG TTGACTTTTA CCGATGCACC TGTCCATATG GTTTCAAGGG GCAGGACTGT 
GATGTCCCAA TTCATGCCTG CATCAGTAAC CCATGTAAAC ATGGAGGAAC TTGCCACTTA 
AAGGAAGGAG AAGAAGATGG ATTCTGGTGT ATTTGTGCTG ATGGATTTGA AGGAGAAAAT 
TGTGAAGTCA ACGTTGATGA TTGTGAAGAT AATGACTGTG AAAATAATTC TACATGTGTC 
GATGGCATTA ATAACTACAC ATGCCTTTGC CCACCTGAGT ATACAGGTGA GTTGTGTGAG 
GAGAAGCTGG ACTTCTGTGC CCAGGACCTG AACCCCTGCC AGCACGATTC AAAGTGCATC 
CTAACTCCAA AGGGATTCAA ATGTGACTGC ACACCAGGGT ACGTAGGTGA ACACTGCGAC 
ATCGATTTTG ACGACTGCCA AGACAACAAG TGTAAAAACG GAGCCCACTG CACAGATGCA 
GTGAACGGCT ATACGTGCAT ATGCCCCGAA GGTTACAGTG GCTTGTTCTG TGAGTTTTCT 
CCACCCATGG TCCTCCCTCG TACCAGCCCC TGTGATAATT TTGATTGTCA GAATGGAGCT 
CAGTGTATCG TCAGAATAAA TGAGCCAATA TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA 
AAGTGTGAAA AATTGGTTAG TGTGAATTTT ATAAACAAAG AGTCTTATCT TCAGATTCCT 
TCAGCCAAGG TTCGGCCTCA GACGAACATA ACACTTCAGA TTGCCACAGA TGAAGACAGC 
GGAATCCTCC TGTATAAGGG TGACAAAGAC CATATCGCGG TAGAACTCTA TCGGGGGCGT 
GTTCGTGCCA GCTATGACAC CGGCTCTCAT CCAGCTTCTG CCATTTACAG TGTGGAGACA 
ATCAATGATG GAAACTTCCA CATTGTGGAA CTACTTGCCT TGGATCAGAG TCTCTCTTTG 
TCCGTGGATG GTGGGAACCC CAAAATCATC ACTAACTTGT CAAAGCAGTC CACTCTGAAT 
TTTGACTCTC CACTCTATGT AGGAGGCATG CCAGGGAAGA GTAACGTGGC ATCTCTGCGC 
CAGGCCCCTG GGCAGAACGG AACCAGCTTC CACGGCTGCA TCCGGAACCT TTACATCAAC 
AGTGAGCTGC AGGACTTCCA GAAGGTGCCG ATGCAAACAG GCATTTTGCC TGGCTGTGAG 
CCATGCCACA AGAAGGTGTG TGCCCATGGC ACATGCCAGC CCAGCAGCCA GGCAGGCTTC 
ACCTGCGAGT GCCAGGAAGG ATGGATGGGG CCCCTCTGTG ACCAACGGAC CAATGACCCT 
TGCCTTGGAA ATAAATGCGT ACATGGCACC TGCTTGCCCA TCAATGCGTT CTCCTACAGC 
TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC CTCTGTGATG AAGAGGAGGA TCTGTTTAAC 
CCATGCCAGG CGATCAAGTG CAAGCATGGG AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC 
TACTGTGAAT GCAGCAGTGG ATACACGGGG GACAGCTGTG ATCGAGAAAT CTCTTGTCGA 
GGGGAAAGGA TAAGAGATTA TTACCAAAAG CAGCAGGGCT ATGCTGCTTG CCAAACAACC 
AAGAAGGTGT CCCGATTAGA GTGCAGAGGT GGGTGTGCAG GAGGGCAGTG CTGTGGACCG 
CTGAGGAGCA AGCGGCGGAA ATACTCTTTC GAATGCACTG ACGGCTCCTC CTTTGTGGAC 
GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864334.1
CDS	41..4528
Translation	MPGASETPLCSPESINGVTLRLPASRDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	XM_017008845.1
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008845.1

ORF Insert Sequence:

ATGCCCGGGG CAAGTGAGAC GCCACTTTGT TCTCCAGAGT CCATAAACGG AGTCACCTTG 
CGATTGCCAG CATCCAGGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT 
TTTGCTGGTC TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT 
GAAAGAGGAG CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC 
CTTCAGCTGT TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC 
AGTGAAAACC AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA 
AATTTGCAAC TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC 
CGGGACCTGG AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT 
TTCAACCATA TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC 
TGCCACCTGG CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT 
CAGTGTATGG GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA 
TTTGTCTGCA GTGGTCACCA GTCATTTATG GCTCCTTCTT GTAGTGTTTT GCACTGCCCT 
GCCGCCTGTA CCTGTAGCAA CAATATCGTA GACTGTCGTG GGAAAGGTCT CACTGAGATC 
CCCACAAATC TTCCAGAGAC CATCACAGAA ATACGTTTGG AACAGAACAC AATCAAAGTC 
ATCCCTCCTG GAGCTTTCTC ACCATATAAA AAGCTTAGAC GAATTGACCT GAGCAATAAT 
CAGATCTCTG AACTTGCACC AGATGCTTTC CAAGGACTAC GCTCTCTGAA TTCACTTGTC 
CTCTATGGAA ATAAAATCAC AGAACTCCCC AAAAGTTTAT TTGAAGGACT GTTTTCCTTA 
CAGCTCCTAT TATTGAATGC CAACAAGATA AACTGCCTTC GGGTAGATGC TTTTCAGGAT 
CTCCACAACT TGAACCTTCT CTCCCTATAT GACAACAAGC TTCAGACCAT CGCCAAGGGG 
ACCTTTTCAC CTCTTCGGGC CATTCAAACT ATGCATTTGG CCCAGAACCC CTTTATTTGT 
GACTGCCATC TCAAGTGGCT AGCGGATTAT CTCCATACCA ACCCGATTGA GACCAGTGGT 
GCCCGTTGCA CCAGCCCCCG CCGCCTGGCA AACAAAAGAA TTGGACAGAT CAAAAGCAAG 
AAATTCCGTT GTTCAGCTAA AGAACAGTAT TTCATTCCAG GTACAGAAGA TTATCGATCA 
AAATTAAGTG GAGACTGCTT TGCGGATCTG GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA 
ACCACAGTAG ATTGCTCTAA TCAAAAGCTC AACAAAATCC CGGAGCACAT TCCCCAGTAC 
ACTGCAGAGT TGCGTCTCAA TAATAATGAA TTTACCGTGT TGGAAGCCAC AGGAATCTTT 
AAGAAACTTC CTCAATTACG TAAAATAAAC TTTAGCAACA ATAAGATCAC AGATATTGAG 
GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA AATGAAATAC TTCTTACGAG TAATCGTTTG 
GAAAATGTGC AGCATAAGAT GTTCAAGGGA TTGGAAAGCC TCAAAACTTT GATGTTGAGA 
AGCAATCGAA TAACCTGTGT GGGGAATGAC AGTTTCATAG GACTCAGTTC TGTGCGTTTG 
CTTTCTTTGT ATGATAATCA AATTACTACA GTTGCACCAG GGGCATTTGA TACTCTCCAT 
TCTTTATCTA CTCTAAACCT CTTGGCCAAT CCTTTTAACT GTAACTGCTA CCTGGCTTGG 
TTGGGAGAGT GGCTGAGAAA GAAGAGAATT GTCACGGGAA ATCCTAGATG TCAAAAACCA 
TACTTCCTGA AAGAAATACC CATCCAGGAT GTGGCCATTC AGGACTTCAC TTGTGATGAC 
GGAAATGATG ACAATAGTTG CTCCCCACTT TCTCGCTGTC CTACTGAATG TACTTGCTTG 
GATACAGTCG TCCGATGTAG CAACAAGGGT TTGAAGGTCT TGCCGAAAGG TATTCCAAGA 
GATGTCACAG AGTTGTATCT GGATGGAAAC CAATTTACAC TGGTTCCCAA GGAACTCTCC 
AACTACAAAC ATTTAACACT TATAGACTTA AGTAACAACA GAATAAGCAC GCTTTCTAAT 
CAGAGCTTCA GCAACATGAC CCAGCTCCTC ACCTTAATTC TTAGTTACAA CCGTCTGAGA 
TGTATTCCTC CTCGCACCTT TGATGGATTA AAGTCTCTTC GATTACTTTC TCTACATGGA 
AATGACATTT CTGTTGTGCC TGAAGGTGCT TTCAATGATC TTTCTGCATT ATCACATCTA 
GCAATTGGAG CCAACCCTCT TTACTGTGAT TGTAACATGC AGTGGTTATC CGACTGGGTG 
AAGTCGGAAT ATAAGGAGCC TGGAATTGCT CGTTGTGCTG GTCCTGGAGA AATGGCAGAT 
AAACTTTTAC TCACAACTCC CTCCAAAAAA TTTACCTGTC AAGGTCCTGT GGATGTCAAT 
ATTCTAGCTA AGTGTAACCC CTGCCTATCA AATCCGTGTA AAAATGATGG CACATGTAAT 
AGTGATCCAG TTGACTTTTA CCGATGCACC TGTCCATATG GTTTCAAGGG GCAGGACTGT 
GATGTCCCAA TTCATGCCTG CATCAGTAAC CCATGTAAAC ATGGAGGAAC TTGCCACTTA 
AAGGAAGGAG AAGAAGATGG ATTCTGGTGT ATTTGTGCTG ATGGATTTGA AGGAGAAAAT 
TGTGAAGTCA ACGTTGATGA TTGTGAAGAT AATGACTGTG AAAATAATTC TACATGTGTC 
GATGGCATTA ATAACTACAC ATGCCTTTGC CCACCTGAGT ATACAGGTGA GTTGTGTGAG 
GAGAAGCTGG ACTTCTGTGC CCAGGACCTG AACCCCTGCC AGCACGATTC AAAGTGCATC 
CTAACTCCAA AGGGATTCAA ATGTGACTGC ACACCAGGGT ACGTAGGTGA ACACTGCGAC 
ATCGATTTTG ACGACTGCCA AGACAACAAG TGTAAAAACG GAGCCCACTG CACAGATGCA 
GTGAACGGCT ATACGTGCAT ATGCCCCGAA GGTTACAGTG GCTTGTTCTG TGAGTTTTCT 
CCACCCATGG TCCTCCCTCG TACCAGCCCC TGTGATAATT TTGATTGTCA GAATGGAGCT 
CAGTGTATCG TCAGAATAAA TGAGCCAATA TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA 
AAGTGTGAAA AATTGGTTAG TGTGAATTTT ATAAACAAAG AGTCTTATCT TCAGATTCCT 
TCAGCCAAGG TTCGGCCTCA GACGAACATA ACACTTCAGA TTGCCACAGA TGAAGACAGC 
GGAATCCTCC TGTATAAGGG TGACAAAGAC CATATCGCGG TAGAACTCTA TCGGGGGCGT 
GTTCGTGCCA GCTATGACAC CGGCTCTCAT CCAGCTTCTG CCATTTACAG TGTGGAGACA 
ATCAATGATG GAAACTTCCA CATTGTGGAA CTACTTGCCT TGGATCAGAG TCTCTCTTTG 
TCCGTGGATG GTGGGAACCC CAAAATCATC ACTAACTTGT CAAAGCAGTC CACTCTGAAT 
TTTGACTCTC CACTCTATGT AGGAGGCATG CCAGGGAAGA GTAACGTGGC ATCTCTGCGC 
CAGGCCCCTG GGCAGAACGG AACCAGCTTC CACGGCTGCA TCCGGAACCT TTACATCAAC 
AGTGAGCTGC AGGACTTCCA GAAGGTGCCG ATGCAAACAG GCATTTTGCC TGGCTGTGAG 
CCATGCCACA AGAAGGTGTG TGCCCATGGC ACATGCCAGC CCAGCAGCCA GGCAGGCTTC 
ACCTGCGAGT GCCAGGAAGG ATGGATGGGG CCCCTCTGTG ACCAACGGAC CAATGACCCT 
TGCCTTGGAA ATAAATGCGT ACATGGCACC TGCTTGCCCA TCAATGCGTT CTCCTACAGC 
TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC CTCTGTGATG AAGAGGAGGA TCTGTTTAAC 
CCATGCCAGG CGATCAAGTG CAAGCATGGG AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC 
TACTGTGAAT GCAGCAGTGG ATACACGGGG GACAGCTGTG ATCGAGAAAT CTCTTGTCGA 
GGGGAAAGGA TAAGAGATTA TTACCAAAAG CAGCAGGGCT ATGCTGCTTG CCAAACAACC 
AAGAAGGTGT CCCGATTAGA GTGCAGAGGT GGGTGTGCAG GAGGGCAGTG CTGTGGACCG 
CTGAGGAGCA AGCGGCGGAA ATACTCTTTC GAATGCACTG ACGGCTCCTC CTTTGTGGAC 
GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_011513910.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512212.2 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

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CloneID	OHu95552
Clone ID Related Accession (Same CDS sequence)	XM_011513910.2
Accession Version	XM_011513910.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTATC CAAGTATTGC CTACAGTTTC TCCCTCTTAA GGCGGGAATT TAAAAAAATT 
TCTCCATTCC TATGGAGAAA AATATTCTTT CTCTCCTTAT ATAATAACAC GCTTCAGACC 
ATCACCAAAG GGACCTTTTC ACCTCTTCGG GCCATTCAAA CTATGCATTT GGCCCAGAAC 
CCCTTTATTT GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCATAC CAACCCGATT 
GAGACCAGTG GTGCCCGTTG CACCAGCCCC CGCCGCCTGG CAAACAAAAG AATTGGACAG 
ATCAAAAGCA AGAAATTCCG TTGTTCAGCT AAAGAACAGT ATTTCATTCC AGGTACAGAA 
GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCGGATC TGGCTTGCCC TGAAAAGTGT 
CGCTGTGAAG GAACCACAGT AGATTGCTCT AATCAAAAGC TCAACAAAAT CCCGGAGCAC 
ATTCCCCAGT ACACTGCAGA GTTGCGTCTC AATAATAATG AATTTACCGT GTTGGAAGCC 
ACAGGAATCT TTAAGAAACT TCCTCAATTA CGTAAAATAA ACTTTAGCAA CAATAAGATC 
ACAGATATTG AGGAGGGAGC ATTTGAAGGA GCATCTGGTG TAAATGAAAT ACTTCTTACG 
AGTAATCGTT TGGAAAATGT GCAGCATAAG ATGTTCAAGG GATTGGAAAG CCTCAAAACT 
TTGATGTTGA GAAGCAATCG AATAACCTGT GTGGGGAATG ACAGTTTCAT AGGACTCAGT 
TCTGTGCGTT TGCTTTCTTT GTATGATAAT CAAATTACTA CAGTTGCACC AGGGGCATTT 
GATACTCTCC ATTCTTTATC TACTCTAAAC CTCTTGGCCA ATCCTTTTAA CTGTAACTGC 
TACCTGGCTT GGTTGGGAGA GTGGCTGAGA AAGAAGAGAA TTGTCACGGG AAATCCTAGA 
TGTCAAAAAC CATACTTCCT GAAAGAAATA CCCATCCAGG ATGTGGCCAT TCAGGACTTC 
ACTTGTGATG ACGGAAATGA TGACAATAGT TGCTCCCCAC TTTCTCGCTG TCCTACTGAA 
TGTACTTGCT TGGATACAGT CGTCCGATGT AGCAACAAGG GTTTGAAGGT CTTGCCGAAA 
GGTATTCCAA GAGATGTCAC AGAGTTGTAT CTGGATGGAA ACCAATTTAC ACTGGTTCCC 
AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC 
ACGCTTTCTA ATCAGAGCTT CAGCAACATG ACCCAGCTCC TCACCTTAAT TCTTAGTTAC 
AACCGTCTGA GATGTATTCC TCCTCGCACC TTTGATGGAT TAAAGTCTCT TCGATTACTT 
TCTCTACATG GAAATGACAT TTCTGTTGTG CCTGAAGGTG CTTTCAATGA TCTTTCTGCA 
TTATCACATC TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA 
TCCGACTGGG TGAAGTCGGA ATATAAGGAG CCTGGAATTG CTCGTTGTGC TGGTCCTGGA 
GAAATGGCAG ATAAACTTTT ACTCACAACT CCCTCCAAAA AATTTACCTG TCAAGGTCCT 
GTGGATGTCA ATATTCTAGC TAAGTGTAAC CCCTGCCTAT CAAATCCGTG TAAAAATGAT 
GGCACATGTA ATAGTGATCC AGTTGACTTT TACCGATGCA CCTGTCCATA TGGTTTCAAG 
GGGCAGGACT GTGATGTCCC AATTCATGCC TGCATCAGTA ACCCATGTAA ACATGGAGGA 
ACTTGCCACT TAAAGGAAGG AGAAGAAGAT GGATTCTGGT GTATTTGTGC TGATGGATTT 
GAAGGAGAAA ATTGTGAAGT CAACGTTGAT GATTGTGAAG ATAATGACTG TGAAAATAAT 
TCTACATGTG TCGATGGCAT TAATAACTAC ACATGCCTTT GCCCACCTGA GTATACAGGT 
GAGTTGTGTG AGGAGAAGCT GGACTTCTGT GCCCAGGACC TGAACCCCTG CCAGCACGAT 
TCAAAGTGCA TCCTAACTCC AAAGGGATTC AAATGTGACT GCACACCAGG GTACGTAGGT 
GAACACTGCG ACATCGATTT TGACGACTGC CAAGACAACA AGTGTAAAAA CGGAGCCCAC 
TGCACAGATG CAGTGAACGG CTATACGTGC ATATGCCCCG AAGGTTACAG TGGCTTGTTC 
TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGTACCAGCC CCTGTGATAA TTTTGATTGT 
CAGAATGGAG CTCAGTGTAT CGTCAGAATA AATGAGCCAA TATGTCAGTG TTTGCCTGGC 
TATCAGGGAG AAAAGTGTGA AAAATTGGTT AGTGTGAATT TTATAAACAA AGAGTCTTAT 
CTTCAGATTC CTTCAGCCAA GGTTCGGCCT CAGACGAACA TAACACTTCA GATTGCCACA 
GATGAAGACA GCGGAATCCT CCTGTATAAG GGTGACAAAG ACCATATCGC GGTAGAACTC 
TATCGGGGGC GTGTTCGTGC CAGCTATGAC ACCGGCTCTC ATCCAGCTTC TGCCATTTAC 
AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTGG AACTACTTGC CTTGGATCAG 
AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC CCCAAAATCA TCACTAACTT GTCAAAGCAG 
TCCACTCTGA ATTTTGACTC TCCACTCTAT GTAGGAGGCA TGCCAGGGAA GAGTAACGTG 
GCATCTCTGC GCCAGGCCCC TGGGCAGAAC GGAACCAGCT TCCACGGCTG CATCCGGAAC 
CTTTACATCA ACAGTGAGCT GCAGGACTTC CAGAAGGTGC CGATGCAAAC AGGCATTTTG 
CCTGGCTGTG AGCCATGCCA CAAGAAGGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC 
CAGGCAGGCT TCACCTGCGA GTGCCAGGAA GGATGGATGG GGCCCCTCTG TGACCAACGG 
ACCAATGACC CTTGCCTTGG AAATAAATGC GTACATGGCA CCTGCTTGCC CATCAATGCG 
TTCTCCTACA GCTGTAAGTG CTTGGAGGGC CATGGAGGTG TCCTCTGTGA TGAAGAGGAG 
GATCTGTTTA ACCCATGCCA GGCGATCAAG TGCAAGCATG GGAAGTGCAG GCTTTCAGGT 
CTGGGGCAGC CCTACTGTGA ATGCAGCAGT GGATACACGG GGGACAGCTG TGATCGAGAA 
ATCTCTTGTC GAGGGGAAAG GATAAGAGAT TATTACCAAA AGCAGCAGGG CTATGCTGCT 
TGCCAAACAA CCAAGAAGGT GTCCCGATTA GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG 
TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG AAATACTCTT TCGAATGCAC TGACGGCTCC 
TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512212.2
CDS	1914..5393
Translation	MLYPSIAYSFSLLRREFKKISPFLWRKIFFLSLYNNTLQTITKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	XM_011513910.2
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513910.2

ORF Insert Sequence:

ATGCTGTATC CAAGTATTGC CTACAGTTTC TCCCTCTTAA GGCGGGAATT TAAAAAAATT 
TCTCCATTCC TATGGAGAAA AATATTCTTT CTCTCCTTAT ATAATAACAC GCTTCAGACC 
ATCACCAAAG GGACCTTTTC ACCTCTTCGG GCCATTCAAA CTATGCATTT GGCCCAGAAC 
CCCTTTATTT GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCATAC CAACCCGATT 
GAGACCAGTG GTGCCCGTTG CACCAGCCCC CGCCGCCTGG CAAACAAAAG AATTGGACAG 
ATCAAAAGCA AGAAATTCCG TTGTTCAGCT AAAGAACAGT ATTTCATTCC AGGTACAGAA 
GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCGGATC TGGCTTGCCC TGAAAAGTGT 
CGCTGTGAAG GAACCACAGT AGATTGCTCT AATCAAAAGC TCAACAAAAT CCCGGAGCAC 
ATTCCCCAGT ACACTGCAGA GTTGCGTCTC AATAATAATG AATTTACCGT GTTGGAAGCC 
ACAGGAATCT TTAAGAAACT TCCTCAATTA CGTAAAATAA ACTTTAGCAA CAATAAGATC 
ACAGATATTG AGGAGGGAGC ATTTGAAGGA GCATCTGGTG TAAATGAAAT ACTTCTTACG 
AGTAATCGTT TGGAAAATGT GCAGCATAAG ATGTTCAAGG GATTGGAAAG CCTCAAAACT 
TTGATGTTGA GAAGCAATCG AATAACCTGT GTGGGGAATG ACAGTTTCAT AGGACTCAGT 
TCTGTGCGTT TGCTTTCTTT GTATGATAAT CAAATTACTA CAGTTGCACC AGGGGCATTT 
GATACTCTCC ATTCTTTATC TACTCTAAAC CTCTTGGCCA ATCCTTTTAA CTGTAACTGC 
TACCTGGCTT GGTTGGGAGA GTGGCTGAGA AAGAAGAGAA TTGTCACGGG AAATCCTAGA 
TGTCAAAAAC CATACTTCCT GAAAGAAATA CCCATCCAGG ATGTGGCCAT TCAGGACTTC 
ACTTGTGATG ACGGAAATGA TGACAATAGT TGCTCCCCAC TTTCTCGCTG TCCTACTGAA 
TGTACTTGCT TGGATACAGT CGTCCGATGT AGCAACAAGG GTTTGAAGGT CTTGCCGAAA 
GGTATTCCAA GAGATGTCAC AGAGTTGTAT CTGGATGGAA ACCAATTTAC ACTGGTTCCC 
AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC 
ACGCTTTCTA ATCAGAGCTT CAGCAACATG ACCCAGCTCC TCACCTTAAT TCTTAGTTAC 
AACCGTCTGA GATGTATTCC TCCTCGCACC TTTGATGGAT TAAAGTCTCT TCGATTACTT 
TCTCTACATG GAAATGACAT TTCTGTTGTG CCTGAAGGTG CTTTCAATGA TCTTTCTGCA 
TTATCACATC TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA 
TCCGACTGGG TGAAGTCGGA ATATAAGGAG CCTGGAATTG CTCGTTGTGC TGGTCCTGGA 
GAAATGGCAG ATAAACTTTT ACTCACAACT CCCTCCAAAA AATTTACCTG TCAAGGTCCT 
GTGGATGTCA ATATTCTAGC TAAGTGTAAC CCCTGCCTAT CAAATCCGTG TAAAAATGAT 
GGCACATGTA ATAGTGATCC AGTTGACTTT TACCGATGCA CCTGTCCATA TGGTTTCAAG 
GGGCAGGACT GTGATGTCCC AATTCATGCC TGCATCAGTA ACCCATGTAA ACATGGAGGA 
ACTTGCCACT TAAAGGAAGG AGAAGAAGAT GGATTCTGGT GTATTTGTGC TGATGGATTT 
GAAGGAGAAA ATTGTGAAGT CAACGTTGAT GATTGTGAAG ATAATGACTG TGAAAATAAT 
TCTACATGTG TCGATGGCAT TAATAACTAC ACATGCCTTT GCCCACCTGA GTATACAGGT 
GAGTTGTGTG AGGAGAAGCT GGACTTCTGT GCCCAGGACC TGAACCCCTG CCAGCACGAT 
TCAAAGTGCA TCCTAACTCC AAAGGGATTC AAATGTGACT GCACACCAGG GTACGTAGGT 
GAACACTGCG ACATCGATTT TGACGACTGC CAAGACAACA AGTGTAAAAA CGGAGCCCAC 
TGCACAGATG CAGTGAACGG CTATACGTGC ATATGCCCCG AAGGTTACAG TGGCTTGTTC 
TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGTACCAGCC CCTGTGATAA TTTTGATTGT 
CAGAATGGAG CTCAGTGTAT CGTCAGAATA AATGAGCCAA TATGTCAGTG TTTGCCTGGC 
TATCAGGGAG AAAAGTGTGA AAAATTGGTT AGTGTGAATT TTATAAACAA AGAGTCTTAT 
CTTCAGATTC CTTCAGCCAA GGTTCGGCCT CAGACGAACA TAACACTTCA GATTGCCACA 
GATGAAGACA GCGGAATCCT CCTGTATAAG GGTGACAAAG ACCATATCGC GGTAGAACTC 
TATCGGGGGC GTGTTCGTGC CAGCTATGAC ACCGGCTCTC ATCCAGCTTC TGCCATTTAC 
AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTGG AACTACTTGC CTTGGATCAG 
AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC CCCAAAATCA TCACTAACTT GTCAAAGCAG 
TCCACTCTGA ATTTTGACTC TCCACTCTAT GTAGGAGGCA TGCCAGGGAA GAGTAACGTG 
GCATCTCTGC GCCAGGCCCC TGGGCAGAAC GGAACCAGCT TCCACGGCTG CATCCGGAAC 
CTTTACATCA ACAGTGAGCT GCAGGACTTC CAGAAGGTGC CGATGCAAAC AGGCATTTTG 
CCTGGCTGTG AGCCATGCCA CAAGAAGGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC 
CAGGCAGGCT TCACCTGCGA GTGCCAGGAA GGATGGATGG GGCCCCTCTG TGACCAACGG 
ACCAATGACC CTTGCCTTGG AAATAAATGC GTACATGGCA CCTGCTTGCC CATCAATGCG 
TTCTCCTACA GCTGTAAGTG CTTGGAGGGC CATGGAGGTG TCCTCTGTGA TGAAGAGGAG 
GATCTGTTTA ACCCATGCCA GGCGATCAAG TGCAAGCATG GGAAGTGCAG GCTTTCAGGT 
CTGGGGCAGC CCTACTGTGA ATGCAGCAGT GGATACACGG GGGACAGCTG TGATCGAGAA 
ATCTCTTGTC GAGGGGAAAG GATAAGAGAT TATTACCAAA AGCAGCAGGG CTATGCTGCT 
TGCCAAACAA CCAAGAAGGT GTCCCGATTA GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG 
TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG AAATACTCTT TCGAATGCAC TGACGGCTCC 
TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_001289135.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276064.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.

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CloneID	OHu22199
Clone ID Related Accession (Same CDS sequence)	NM_001289135.2 , NM_001289135.3
Accession Version	NM_001289135.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC143978.1, AF133270.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. On Sep 10, 2015 this sequence version replaced NM_001289135.1. Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks one alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.151074.1, AF133270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCGGATCTG 
GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA ACCACAGTAG ATTGCTCTAA TCAAAAGCTC 
AACAAAATCC CGGAGCACAT TCCCCAGTAC ACTGCAGAGT TGCGTCTCAA TAATAATGAA 
TTTACCGTGT TGGAAGCCAC AGGAATCTTT AAGAAACTTC CTCAATTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGATATTGAG GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA 
AATGAAATAC TTCTTACGAG TAATCGTTTG GAAAATGTGC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TCAAAACTTT GATGTTGAGA AGCAATCGAA TAACCTGTGT GGGGAATGAC 
AGTTTCATAG GACTCAGTTC TGTGCGTTTG CTTTCTTTGT ATGATAATCA AATTACTACA 
GTTGCACCAG GGGCATTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTTAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAGT GGCTGAGAAA GAAGAGAATT 
GTCACGGGAA ATCCTAGATG TCAAAAACCA TACTTCCTGA AAGAAATACC CATCCAGGAT 
GTGGCCATTC AGGACTTCAC TTGTGATGAC GGAAATGATG ACAATAGTTG CTCCCCACTT 
TCTCGCTGTC CTACTGAATG TACTTGCTTG GATACAGTCG TCCGATGTAG CAACAAGGGT 
TTGAAGGTCT TGCCGAAAGG TATTCCAAGA GATGTCACAG AGTTGTATCT GGATGGAAAC 
CAATTTACAC TGGTTCCCAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC GCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCTTAATTC TTAGTTACAA CCGTCTGAGA TGTATTCCTC CTCGCACCTT TGATGGATTA 
AAGTCTCTTC GATTACTTTC TCTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTTCTGCATT ATCACATCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAGCC TGGAATTGCT 
CGTTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTTTTAC TCACAACTCC CTCCAAAAAA 
TTTACCTGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AGTGTAACCC CTGCCTATCA 
AATCCGTGTA AAAATGATGG CACATGTAAT AGTGATCCAG TTGACTTTTA CCGATGCACC 
TGTCCATATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TTCATGCCTG CATCAGTAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAGGAAGGAG AAGAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ACGTTGATGA TTGTGAAGAT 
AATGACTGTG AAAATAATTC TACATGTGTC GATGGCATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ATACAGGTGA GTTGTGTGAG GAGAAGCTGG ACTTCTGTGC CCAGGACCTG 
AACCCCTGCC AGCACGATTC AAAGTGCATC CTAACTCCAA AGGGATTCAA ATGTGACTGC 
ACACCAGGGT ACGTAGGTGA ACACTGCGAC ATCGATTTTG ACGACTGCCA AGACAACAAG 
TGTAAAAACG GAGCCCACTG CACAGATGCA GTGAACGGCT ATACGTGCAT ATGCCCCGAA 
GGTTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAGTGTATCG TCAGAATAAA TGAGCCAATA 
TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA AAGTGTGAAA AATTGGTTAG TGTGAATTTT 
ATAAACAAAG AGTCTTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACGAACATA 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGAATCCTCC TGTATAAGGG TGACAAAGAC 
CATATCGCGG TAGAACTCTA TCGGGGGCGT GTTCGTGCCA GCTATGACAC CGGCTCTCAT 
CCAGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAA 
CTACTTGCCT TGGATCAGAG TCTCTCTTTG TCCGTGGATG GTGGGAACCC CAAAATCATC 
ACTAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCAGGGAAGA GTAACGTGGC ATCTCTGCGC CAGGCCCCTG GGCAGAACGG AACCAGCTTC 
CACGGCTGCA TCCGGAACCT TTACATCAAC AGTGAGCTGC AGGACTTCCA GAAGGTGCCG 
ATGCAAACAG GCATTTTGCC TGGCTGTGAG CCATGCCACA AGAAGGTGTG TGCCCATGGC 
ACATGCCAGC CCAGCAGCCA GGCAGGCTTC ACCTGCGAGT GCCAGGAAGG ATGGATGGGG 
CCCCTCTGTG ACCAACGGAC CAATGACCCT TGCCTTGGAA ATAAATGCGT ACATGGCACC 
TGCTTGCCCA TCAATGCGTT CTCCTACAGC TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC 
CTCTGTGATG AAGAGGAGGA TCTGTTTAAC CCATGCCAGG CGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC TACTGTGAAT GCAGCAGTGG ATACACGGGG 
GACAGCTGTG ATCGAGAAAT CTCTTGTCGA GGGGAAAGGA TAAGAGATTA TTACCAAAAG 
CAGCAGGGCT ATGCTGCTTG CCAAACAACC AAGAAGGTGT CCCGATTAGA GTGCAGAGGT 
GGGTGTGCAG GAGGGCAGTG CTGTGGACCG CTGAGGAGCA AGCGGCGGAA ATACTCTTTC 
GAATGCACTG ACGGCTCCTC CTTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT 
ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276064.1
CDS	1912..6489
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_001289135.2
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289135.2

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCGGATCTG 
GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA ACCACAGTAG ATTGCTCTAA TCAAAAGCTC 
AACAAAATCC CGGAGCACAT TCCCCAGTAC ACTGCAGAGT TGCGTCTCAA TAATAATGAA 
TTTACCGTGT TGGAAGCCAC AGGAATCTTT AAGAAACTTC CTCAATTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGATATTGAG GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA 
AATGAAATAC TTCTTACGAG TAATCGTTTG GAAAATGTGC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TCAAAACTTT GATGTTGAGA AGCAATCGAA TAACCTGTGT GGGGAATGAC 
AGTTTCATAG GACTCAGTTC TGTGCGTTTG CTTTCTTTGT ATGATAATCA AATTACTACA 
GTTGCACCAG GGGCATTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTTAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAGT GGCTGAGAAA GAAGAGAATT 
GTCACGGGAA ATCCTAGATG TCAAAAACCA TACTTCCTGA AAGAAATACC CATCCAGGAT 
GTGGCCATTC AGGACTTCAC TTGTGATGAC GGAAATGATG ACAATAGTTG CTCCCCACTT 
TCTCGCTGTC CTACTGAATG TACTTGCTTG GATACAGTCG TCCGATGTAG CAACAAGGGT 
TTGAAGGTCT TGCCGAAAGG TATTCCAAGA GATGTCACAG AGTTGTATCT GGATGGAAAC 
CAATTTACAC TGGTTCCCAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC GCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCTTAATTC TTAGTTACAA CCGTCTGAGA TGTATTCCTC CTCGCACCTT TGATGGATTA 
AAGTCTCTTC GATTACTTTC TCTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTTCTGCATT ATCACATCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAGCC TGGAATTGCT 
CGTTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTTTTAC TCACAACTCC CTCCAAAAAA 
TTTACCTGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AGTGTAACCC CTGCCTATCA 
AATCCGTGTA AAAATGATGG CACATGTAAT AGTGATCCAG TTGACTTTTA CCGATGCACC 
TGTCCATATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TTCATGCCTG CATCAGTAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAGGAAGGAG AAGAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ACGTTGATGA TTGTGAAGAT 
AATGACTGTG AAAATAATTC TACATGTGTC GATGGCATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ATACAGGTGA GTTGTGTGAG GAGAAGCTGG ACTTCTGTGC CCAGGACCTG 
AACCCCTGCC AGCACGATTC AAAGTGCATC CTAACTCCAA AGGGATTCAA ATGTGACTGC 
ACACCAGGGT ACGTAGGTGA ACACTGCGAC ATCGATTTTG ACGACTGCCA AGACAACAAG 
TGTAAAAACG GAGCCCACTG CACAGATGCA GTGAACGGCT ATACGTGCAT ATGCCCCGAA 
GGTTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAGTGTATCG TCAGAATAAA TGAGCCAATA 
TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA AAGTGTGAAA AATTGGTTAG TGTGAATTTT 
ATAAACAAAG AGTCTTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACGAACATA 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGAATCCTCC TGTATAAGGG TGACAAAGAC 
CATATCGCGG TAGAACTCTA TCGGGGGCGT GTTCGTGCCA GCTATGACAC CGGCTCTCAT 
CCAGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAA 
CTACTTGCCT TGGATCAGAG TCTCTCTTTG TCCGTGGATG GTGGGAACCC CAAAATCATC 
ACTAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCAGGGAAGA GTAACGTGGC ATCTCTGCGC CAGGCCCCTG GGCAGAACGG AACCAGCTTC 
CACGGCTGCA TCCGGAACCT TTACATCAAC AGTGAGCTGC AGGACTTCCA GAAGGTGCCG 
ATGCAAACAG GCATTTTGCC TGGCTGTGAG CCATGCCACA AGAAGGTGTG TGCCCATGGC 
ACATGCCAGC CCAGCAGCCA GGCAGGCTTC ACCTGCGAGT GCCAGGAAGG ATGGATGGGG 
CCCCTCTGTG ACCAACGGAC CAATGACCCT TGCCTTGGAA ATAAATGCGT ACATGGCACC 
TGCTTGCCCA TCAATGCGTT CTCCTACAGC TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC 
CTCTGTGATG AAGAGGAGGA TCTGTTTAAC CCATGCCAGG CGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC TACTGTGAAT GCAGCAGTGG ATACACGGGG 
GACAGCTGTG ATCGAGAAAT CTCTTGTCGA GGGGAAAGGA TAAGAGATTA TTACCAAAAG 
CAGCAGGGCT ATGCTGCTTG CCAAACAACC AAGAAGGTGT CCCGATTAGA GTGCAGAGGT 
GGGTGTGCAG GAGGGCAGTG CTGTGGACCG CTGAGGAGCA AGCGGCGGAA ATACTCTTTC 
GAATGCACTG ACGGCTCCTC CTTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT 
ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_001289135.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276064.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.

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CloneID	OHu22199
Clone ID Related Accession (Same CDS sequence)	NM_001289135.2 , NM_001289135.3
Accession Version	NM_001289135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC143978.1, AF133270.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. On May 31, 2019 this sequence version replaced NM_001289135.2. Transcript Variant: This variant (2) contains one alternate in-frame exon and lacks one alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.151074.1, AF133270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCGGATCTG 
GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA ACCACAGTAG ATTGCTCTAA TCAAAAGCTC 
AACAAAATCC CGGAGCACAT TCCCCAGTAC ACTGCAGAGT TGCGTCTCAA TAATAATGAA 
TTTACCGTGT TGGAAGCCAC AGGAATCTTT AAGAAACTTC CTCAATTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGATATTGAG GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA 
AATGAAATAC TTCTTACGAG TAATCGTTTG GAAAATGTGC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TCAAAACTTT GATGTTGAGA AGCAATCGAA TAACCTGTGT GGGGAATGAC 
AGTTTCATAG GACTCAGTTC TGTGCGTTTG CTTTCTTTGT ATGATAATCA AATTACTACA 
GTTGCACCAG GGGCATTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTTAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAGT GGCTGAGAAA GAAGAGAATT 
GTCACGGGAA ATCCTAGATG TCAAAAACCA TACTTCCTGA AAGAAATACC CATCCAGGAT 
GTGGCCATTC AGGACTTCAC TTGTGATGAC GGAAATGATG ACAATAGTTG CTCCCCACTT 
TCTCGCTGTC CTACTGAATG TACTTGCTTG GATACAGTCG TCCGATGTAG CAACAAGGGT 
TTGAAGGTCT TGCCGAAAGG TATTCCAAGA GATGTCACAG AGTTGTATCT GGATGGAAAC 
CAATTTACAC TGGTTCCCAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC GCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCTTAATTC TTAGTTACAA CCGTCTGAGA TGTATTCCTC CTCGCACCTT TGATGGATTA 
AAGTCTCTTC GATTACTTTC TCTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTTCTGCATT ATCACATCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAGCC TGGAATTGCT 
CGTTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTTTTAC TCACAACTCC CTCCAAAAAA 
TTTACCTGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AGTGTAACCC CTGCCTATCA 
AATCCGTGTA AAAATGATGG CACATGTAAT AGTGATCCAG TTGACTTTTA CCGATGCACC 
TGTCCATATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TTCATGCCTG CATCAGTAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAGGAAGGAG AAGAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ACGTTGATGA TTGTGAAGAT 
AATGACTGTG AAAATAATTC TACATGTGTC GATGGCATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ATACAGGTGA GTTGTGTGAG GAGAAGCTGG ACTTCTGTGC CCAGGACCTG 
AACCCCTGCC AGCACGATTC AAAGTGCATC CTAACTCCAA AGGGATTCAA ATGTGACTGC 
ACACCAGGGT ACGTAGGTGA ACACTGCGAC ATCGATTTTG ACGACTGCCA AGACAACAAG 
TGTAAAAACG GAGCCCACTG CACAGATGCA GTGAACGGCT ATACGTGCAT ATGCCCCGAA 
GGTTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAGTGTATCG TCAGAATAAA TGAGCCAATA 
TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA AAGTGTGAAA AATTGGTTAG TGTGAATTTT 
ATAAACAAAG AGTCTTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACGAACATA 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGAATCCTCC TGTATAAGGG TGACAAAGAC 
CATATCGCGG TAGAACTCTA TCGGGGGCGT GTTCGTGCCA GCTATGACAC CGGCTCTCAT 
CCAGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAA 
CTACTTGCCT TGGATCAGAG TCTCTCTTTG TCCGTGGATG GTGGGAACCC CAAAATCATC 
ACTAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCAGGGAAGA GTAACGTGGC ATCTCTGCGC CAGGCCCCTG GGCAGAACGG AACCAGCTTC 
CACGGCTGCA TCCGGAACCT TTACATCAAC AGTGAGCTGC AGGACTTCCA GAAGGTGCCG 
ATGCAAACAG GCATTTTGCC TGGCTGTGAG CCATGCCACA AGAAGGTGTG TGCCCATGGC 
ACATGCCAGC CCAGCAGCCA GGCAGGCTTC ACCTGCGAGT GCCAGGAAGG ATGGATGGGG 
CCCCTCTGTG ACCAACGGAC CAATGACCCT TGCCTTGGAA ATAAATGCGT ACATGGCACC 
TGCTTGCCCA TCAATGCGTT CTCCTACAGC TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC 
CTCTGTGATG AAGAGGAGGA TCTGTTTAAC CCATGCCAGG CGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC TACTGTGAAT GCAGCAGTGG ATACACGGGG 
GACAGCTGTG ATCGAGAAAT CTCTTGTCGA GGGGAAAGGA TAAGAGATTA TTACCAAAAG 
CAGCAGGGCT ATGCTGCTTG CCAAACAACC AAGAAGGTGT CCCGATTAGA GTGCAGAGGT 
GGGTGTGCAG GAGGGCAGTG CTGTGGACCG CTGAGGAGCA AGCGGCGGAA ATACTCTTTC 
GAATGCACTG ACGGCTCCTC CTTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT 
ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276064.1
CDS	1912..6489
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_001289135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289135.3

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCGGATCTG 
GCTTGCCCTG AAAAGTGTCG CTGTGAAGGA ACCACAGTAG ATTGCTCTAA TCAAAAGCTC 
AACAAAATCC CGGAGCACAT TCCCCAGTAC ACTGCAGAGT TGCGTCTCAA TAATAATGAA 
TTTACCGTGT TGGAAGCCAC AGGAATCTTT AAGAAACTTC CTCAATTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGATATTGAG GAGGGAGCAT TTGAAGGAGC ATCTGGTGTA 
AATGAAATAC TTCTTACGAG TAATCGTTTG GAAAATGTGC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TCAAAACTTT GATGTTGAGA AGCAATCGAA TAACCTGTGT GGGGAATGAC 
AGTTTCATAG GACTCAGTTC TGTGCGTTTG CTTTCTTTGT ATGATAATCA AATTACTACA 
GTTGCACCAG GGGCATTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTTAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAGT GGCTGAGAAA GAAGAGAATT 
GTCACGGGAA ATCCTAGATG TCAAAAACCA TACTTCCTGA AAGAAATACC CATCCAGGAT 
GTGGCCATTC AGGACTTCAC TTGTGATGAC GGAAATGATG ACAATAGTTG CTCCCCACTT 
TCTCGCTGTC CTACTGAATG TACTTGCTTG GATACAGTCG TCCGATGTAG CAACAAGGGT 
TTGAAGGTCT TGCCGAAAGG TATTCCAAGA GATGTCACAG AGTTGTATCT GGATGGAAAC 
CAATTTACAC TGGTTCCCAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC GCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCTTAATTC TTAGTTACAA CCGTCTGAGA TGTATTCCTC CTCGCACCTT TGATGGATTA 
AAGTCTCTTC GATTACTTTC TCTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTTCTGCATT ATCACATCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAGCC TGGAATTGCT 
CGTTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTTTTAC TCACAACTCC CTCCAAAAAA 
TTTACCTGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AGTGTAACCC CTGCCTATCA 
AATCCGTGTA AAAATGATGG CACATGTAAT AGTGATCCAG TTGACTTTTA CCGATGCACC 
TGTCCATATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TTCATGCCTG CATCAGTAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAGGAAGGAG AAGAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ACGTTGATGA TTGTGAAGAT 
AATGACTGTG AAAATAATTC TACATGTGTC GATGGCATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ATACAGGTGA GTTGTGTGAG GAGAAGCTGG ACTTCTGTGC CCAGGACCTG 
AACCCCTGCC AGCACGATTC AAAGTGCATC CTAACTCCAA AGGGATTCAA ATGTGACTGC 
ACACCAGGGT ACGTAGGTGA ACACTGCGAC ATCGATTTTG ACGACTGCCA AGACAACAAG 
TGTAAAAACG GAGCCCACTG CACAGATGCA GTGAACGGCT ATACGTGCAT ATGCCCCGAA 
GGTTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAGTGTATCG TCAGAATAAA TGAGCCAATA 
TGTCAGTGTT TGCCTGGCTA TCAGGGAGAA AAGTGTGAAA AATTGGTTAG TGTGAATTTT 
ATAAACAAAG AGTCTTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACGAACATA 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGAATCCTCC TGTATAAGGG TGACAAAGAC 
CATATCGCGG TAGAACTCTA TCGGGGGCGT GTTCGTGCCA GCTATGACAC CGGCTCTCAT 
CCAGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAA 
CTACTTGCCT TGGATCAGAG TCTCTCTTTG TCCGTGGATG GTGGGAACCC CAAAATCATC 
ACTAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCAGGGAAGA GTAACGTGGC ATCTCTGCGC CAGGCCCCTG GGCAGAACGG AACCAGCTTC 
CACGGCTGCA TCCGGAACCT TTACATCAAC AGTGAGCTGC AGGACTTCCA GAAGGTGCCG 
ATGCAAACAG GCATTTTGCC TGGCTGTGAG CCATGCCACA AGAAGGTGTG TGCCCATGGC 
ACATGCCAGC CCAGCAGCCA GGCAGGCTTC ACCTGCGAGT GCCAGGAAGG ATGGATGGGG 
CCCCTCTGTG ACCAACGGAC CAATGACCCT TGCCTTGGAA ATAAATGCGT ACATGGCACC 
TGCTTGCCCA TCAATGCGTT CTCCTACAGC TGTAAGTGCT TGGAGGGCCA TGGAGGTGTC 
CTCTGTGATG AAGAGGAGGA TCTGTTTAAC CCATGCCAGG CGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCAGGTCT GGGGCAGCCC TACTGTGAAT GCAGCAGTGG ATACACGGGG 
GACAGCTGTG ATCGAGAAAT CTCTTGTCGA GGGGAAAGGA TAAGAGATTA TTACCAAAAG 
CAGCAGGGCT ATGCTGCTTG CCAAACAACC AAGAAGGTGT CCCGATTAGA GTGCAGAGGT 
GGGTGTGCAG GAGGGCAGTG CTGTGGACCG CTGAGGAGCA AGCGGCGGAA ATACTCTTTC 
GAATGCACTG ACGGCTCCTC CTTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGT 
ACGAGGTGTG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_001289136.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276065.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.

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CloneID	OHu22846
Clone ID Related Accession (Same CDS sequence)	NM_001289136.2 , NM_001289136.3
Accession Version	NM_001289136.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC143978.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. On Sep 10, 2015 this sequence version replaced NM_001289136.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CGGATCTGGC TTGCCCTGAA 
AAGTGTCGCT GTGAAGGAAC CACAGTAGAT TGCTCTAATC AAAAGCTCAA CAAAATCCCG 
GAGCACATTC CCCAGTACAC TGCAGAGTTG CGTCTCAATA ATAATGAATT TACCGTGTTG 
GAAGCCACAG GAATCTTTAA GAAACTTCCT CAATTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ATATTGAGGA GGGAGCATTT GAAGGAGCAT CTGGTGTAAA TGAAATACTT 
CTTACGAGTA ATCGTTTGGA AAATGTGCAG CATAAGATGT TCAAGGGATT GGAAAGCCTC 
AAAACTTTGA TGTTGAGAAG CAATCGAATA ACCTGTGTGG GGAATGACAG TTTCATAGGA 
CTCAGTTCTG TGCGTTTGCT TTCTTTGTAT GATAATCAAA TTACTACAGT TGCACCAGGG 
GCATTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTTAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAGTGG CTGAGAAAGA AGAGAATTGT CACGGGAAAT 
CCTAGATGTC AAAAACCATA CTTCCTGAAA GAAATACCCA TCCAGGATGT GGCCATTCAG 
GACTTCACTT GTGATGACGG AAATGATGAC AATAGTTGCT CCCCACTTTC TCGCTGTCCT 
ACTGAATGTA CTTGCTTGGA TACAGTCGTC CGATGTAGCA ACAAGGGTTT GAAGGTCTTG 
CCGAAAGGTA TTCCAAGAGA TGTCACAGAG TTGTATCTGG ATGGAAACCA ATTTACACTG 
GTTCCCAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACGC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CTTAATTCTT 
AGTTACAACC GTCTGAGATG TATTCCTCCT CGCACCTTTG ATGGATTAAA GTCTCTTCGA 
TTACTTTCTC TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
TCTGCATTAT CACATCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAGCCTG GAATTGCTCG TTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTTTTACTC ACAACTCCCT CCAAAAAATT TACCTGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAG TGTAACCCCT GCCTATCAAA TCCGTGTAAA 
AATGATGGCA CATGTAATAG TGATCCAGTT GACTTTTACC GATGCACCTG TCCATATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATT CATGCCTGCA TCAGTAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA GGAAGGAGAA GAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAC GTTGATGATT GTGAAGATAA TGACTGTGAA 
AATAATTCTA CATGTGTCGA TGGCATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAT 
ACAGGTGAGT TGTGTGAGGA GAAGCTGGAC TTCTGTGCCC AGGACCTGAA CCCCTGCCAG 
CACGATTCAA AGTGCATCCT AACTCCAAAG GGATTCAAAT GTGACTGCAC ACCAGGGTAC 
GTAGGTGAAC ACTGCGACAT CGATTTTGAC GACTGCCAAG ACAACAAGTG TAAAAACGGA 
GCCCACTGCA CAGATGCAGT GAACGGCTAT ACGTGCATAT GCCCCGAAGG TTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA GTGTATCGTC AGAATAAATG AGCCAATATG TCAGTGTTTG 
CCTGGCTATC AGGGAGAAAA GTGTGAAAAA TTGGTTAGTG TGAATTTTAT AAACAAAGAG 
TCTTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CGAACATAAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG AATCCTCCTG TATAAGGGTG ACAAAGACCA TATCGCGGTA 
GAACTCTATC GGGGGCGTGT TCGTGCCAGC TATGACACCG GCTCTCATCC AGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAACT ACTTGCCTTG 
GATCAGAGTC TCTCTTTGTC CGTGGATGGT GGGAACCCCA AAATCATCAC TAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC AGGGAAGAGT 
AACGTGGCAT CTCTGCGCCA GGCCCCTGGG CAGAACGGAA CCAGCTTCCA CGGCTGCATC 
CGGAACCTTT ACATCAACAG TGAGCTGCAG GACTTCCAGA AGGTGCCGAT GCAAACAGGC 
ATTTTGCCTG GCTGTGAGCC ATGCCACAAG AAGGTGTGTG CCCATGGCAC ATGCCAGCCC 
AGCAGCCAGG CAGGCTTCAC CTGCGAGTGC CAGGAAGGAT GGATGGGGCC CCTCTGTGAC 
CAACGGACCA ATGACCCTTG CCTTGGAAAT AAATGCGTAC ATGGCACCTG CTTGCCCATC 
AATGCGTTCT CCTACAGCTG TAAGTGCTTG GAGGGCCATG GAGGTGTCCT CTGTGATGAA 
GAGGAGGATC TGTTTAACCC ATGCCAGGCG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCAGGTCTGG GGCAGCCCTA CTGTGAATGC AGCAGTGGAT ACACGGGGGA CAGCTGTGAT 
CGAGAAATCT CTTGTCGAGG GGAAAGGATA AGAGATTATT ACCAAAAGCA GCAGGGCTAT 
GCTGCTTGCC AAACAACCAA GAAGGTGTCC CGATTAGAGT GCAGAGGTGG GTGTGCAGGA 
GGGCAGTGCT GTGGACCGCT GAGGAGCAAG CGGCGGAAAT ACTCTTTCGA ATGCACTGAC 
GGCTCCTCCT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGTAC GAGGTGTGTG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276065.1
CDS	1912..6477
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_001289136.2
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289136.2

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CGGATCTGGC TTGCCCTGAA 
AAGTGTCGCT GTGAAGGAAC CACAGTAGAT TGCTCTAATC AAAAGCTCAA CAAAATCCCG 
GAGCACATTC CCCAGTACAC TGCAGAGTTG CGTCTCAATA ATAATGAATT TACCGTGTTG 
GAAGCCACAG GAATCTTTAA GAAACTTCCT CAATTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ATATTGAGGA GGGAGCATTT GAAGGAGCAT CTGGTGTAAA TGAAATACTT 
CTTACGAGTA ATCGTTTGGA AAATGTGCAG CATAAGATGT TCAAGGGATT GGAAAGCCTC 
AAAACTTTGA TGTTGAGAAG CAATCGAATA ACCTGTGTGG GGAATGACAG TTTCATAGGA 
CTCAGTTCTG TGCGTTTGCT TTCTTTGTAT GATAATCAAA TTACTACAGT TGCACCAGGG 
GCATTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTTAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAGTGG CTGAGAAAGA AGAGAATTGT CACGGGAAAT 
CCTAGATGTC AAAAACCATA CTTCCTGAAA GAAATACCCA TCCAGGATGT GGCCATTCAG 
GACTTCACTT GTGATGACGG AAATGATGAC AATAGTTGCT CCCCACTTTC TCGCTGTCCT 
ACTGAATGTA CTTGCTTGGA TACAGTCGTC CGATGTAGCA ACAAGGGTTT GAAGGTCTTG 
CCGAAAGGTA TTCCAAGAGA TGTCACAGAG TTGTATCTGG ATGGAAACCA ATTTACACTG 
GTTCCCAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACGC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CTTAATTCTT 
AGTTACAACC GTCTGAGATG TATTCCTCCT CGCACCTTTG ATGGATTAAA GTCTCTTCGA 
TTACTTTCTC TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
TCTGCATTAT CACATCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAGCCTG GAATTGCTCG TTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTTTTACTC ACAACTCCCT CCAAAAAATT TACCTGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAG TGTAACCCCT GCCTATCAAA TCCGTGTAAA 
AATGATGGCA CATGTAATAG TGATCCAGTT GACTTTTACC GATGCACCTG TCCATATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATT CATGCCTGCA TCAGTAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA GGAAGGAGAA GAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAC GTTGATGATT GTGAAGATAA TGACTGTGAA 
AATAATTCTA CATGTGTCGA TGGCATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAT 
ACAGGTGAGT TGTGTGAGGA GAAGCTGGAC TTCTGTGCCC AGGACCTGAA CCCCTGCCAG 
CACGATTCAA AGTGCATCCT AACTCCAAAG GGATTCAAAT GTGACTGCAC ACCAGGGTAC 
GTAGGTGAAC ACTGCGACAT CGATTTTGAC GACTGCCAAG ACAACAAGTG TAAAAACGGA 
GCCCACTGCA CAGATGCAGT GAACGGCTAT ACGTGCATAT GCCCCGAAGG TTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA GTGTATCGTC AGAATAAATG AGCCAATATG TCAGTGTTTG 
CCTGGCTATC AGGGAGAAAA GTGTGAAAAA TTGGTTAGTG TGAATTTTAT AAACAAAGAG 
TCTTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CGAACATAAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG AATCCTCCTG TATAAGGGTG ACAAAGACCA TATCGCGGTA 
GAACTCTATC GGGGGCGTGT TCGTGCCAGC TATGACACCG GCTCTCATCC AGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAACT ACTTGCCTTG 
GATCAGAGTC TCTCTTTGTC CGTGGATGGT GGGAACCCCA AAATCATCAC TAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC AGGGAAGAGT 
AACGTGGCAT CTCTGCGCCA GGCCCCTGGG CAGAACGGAA CCAGCTTCCA CGGCTGCATC 
CGGAACCTTT ACATCAACAG TGAGCTGCAG GACTTCCAGA AGGTGCCGAT GCAAACAGGC 
ATTTTGCCTG GCTGTGAGCC ATGCCACAAG AAGGTGTGTG CCCATGGCAC ATGCCAGCCC 
AGCAGCCAGG CAGGCTTCAC CTGCGAGTGC CAGGAAGGAT GGATGGGGCC CCTCTGTGAC 
CAACGGACCA ATGACCCTTG CCTTGGAAAT AAATGCGTAC ATGGCACCTG CTTGCCCATC 
AATGCGTTCT CCTACAGCTG TAAGTGCTTG GAGGGCCATG GAGGTGTCCT CTGTGATGAA 
GAGGAGGATC TGTTTAACCC ATGCCAGGCG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCAGGTCTGG GGCAGCCCTA CTGTGAATGC AGCAGTGGAT ACACGGGGGA CAGCTGTGAT 
CGAGAAATCT CTTGTCGAGG GGAAAGGATA AGAGATTATT ACCAAAAGCA GCAGGGCTAT 
GCTGCTTGCC AAACAACCAA GAAGGTGTCC CGATTAGAGT GCAGAGGTGG GTGTGCAGGA 
GGGCAGTGCT GTGGACCGCT GAGGAGCAAG CGGCGGAAAT ACTCTTTCGA ATGCACTGAC 
GGCTCCTCCT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGTAC GAGGTGTGTG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( NM_001289136.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276065.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.

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CloneID	OHu22846
Clone ID Related Accession (Same CDS sequence)	NM_001289136.2 , NM_001289136.3
Accession Version	NM_001289136.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA721496.1, AC096718.3, BC143978.1, AK126459.1, DB207352.1, CV370469.1, BF112143.1, AC021118.6, BM474839.1, BI494498.1 and AA489463.1. On May 31, 2019 this sequence version replaced NM_001289136.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CGGATCTGGC TTGCCCTGAA 
AAGTGTCGCT GTGAAGGAAC CACAGTAGAT TGCTCTAATC AAAAGCTCAA CAAAATCCCG 
GAGCACATTC CCCAGTACAC TGCAGAGTTG CGTCTCAATA ATAATGAATT TACCGTGTTG 
GAAGCCACAG GAATCTTTAA GAAACTTCCT CAATTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ATATTGAGGA GGGAGCATTT GAAGGAGCAT CTGGTGTAAA TGAAATACTT 
CTTACGAGTA ATCGTTTGGA AAATGTGCAG CATAAGATGT TCAAGGGATT GGAAAGCCTC 
AAAACTTTGA TGTTGAGAAG CAATCGAATA ACCTGTGTGG GGAATGACAG TTTCATAGGA 
CTCAGTTCTG TGCGTTTGCT TTCTTTGTAT GATAATCAAA TTACTACAGT TGCACCAGGG 
GCATTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTTAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAGTGG CTGAGAAAGA AGAGAATTGT CACGGGAAAT 
CCTAGATGTC AAAAACCATA CTTCCTGAAA GAAATACCCA TCCAGGATGT GGCCATTCAG 
GACTTCACTT GTGATGACGG AAATGATGAC AATAGTTGCT CCCCACTTTC TCGCTGTCCT 
ACTGAATGTA CTTGCTTGGA TACAGTCGTC CGATGTAGCA ACAAGGGTTT GAAGGTCTTG 
CCGAAAGGTA TTCCAAGAGA TGTCACAGAG TTGTATCTGG ATGGAAACCA ATTTACACTG 
GTTCCCAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACGC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CTTAATTCTT 
AGTTACAACC GTCTGAGATG TATTCCTCCT CGCACCTTTG ATGGATTAAA GTCTCTTCGA 
TTACTTTCTC TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
TCTGCATTAT CACATCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAGCCTG GAATTGCTCG TTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTTTTACTC ACAACTCCCT CCAAAAAATT TACCTGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAG TGTAACCCCT GCCTATCAAA TCCGTGTAAA 
AATGATGGCA CATGTAATAG TGATCCAGTT GACTTTTACC GATGCACCTG TCCATATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATT CATGCCTGCA TCAGTAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA GGAAGGAGAA GAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAC GTTGATGATT GTGAAGATAA TGACTGTGAA 
AATAATTCTA CATGTGTCGA TGGCATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAT 
ACAGGTGAGT TGTGTGAGGA GAAGCTGGAC TTCTGTGCCC AGGACCTGAA CCCCTGCCAG 
CACGATTCAA AGTGCATCCT AACTCCAAAG GGATTCAAAT GTGACTGCAC ACCAGGGTAC 
GTAGGTGAAC ACTGCGACAT CGATTTTGAC GACTGCCAAG ACAACAAGTG TAAAAACGGA 
GCCCACTGCA CAGATGCAGT GAACGGCTAT ACGTGCATAT GCCCCGAAGG TTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA GTGTATCGTC AGAATAAATG AGCCAATATG TCAGTGTTTG 
CCTGGCTATC AGGGAGAAAA GTGTGAAAAA TTGGTTAGTG TGAATTTTAT AAACAAAGAG 
TCTTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CGAACATAAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG AATCCTCCTG TATAAGGGTG ACAAAGACCA TATCGCGGTA 
GAACTCTATC GGGGGCGTGT TCGTGCCAGC TATGACACCG GCTCTCATCC AGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAACT ACTTGCCTTG 
GATCAGAGTC TCTCTTTGTC CGTGGATGGT GGGAACCCCA AAATCATCAC TAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC AGGGAAGAGT 
AACGTGGCAT CTCTGCGCCA GGCCCCTGGG CAGAACGGAA CCAGCTTCCA CGGCTGCATC 
CGGAACCTTT ACATCAACAG TGAGCTGCAG GACTTCCAGA AGGTGCCGAT GCAAACAGGC 
ATTTTGCCTG GCTGTGAGCC ATGCCACAAG AAGGTGTGTG CCCATGGCAC ATGCCAGCCC 
AGCAGCCAGG CAGGCTTCAC CTGCGAGTGC CAGGAAGGAT GGATGGGGCC CCTCTGTGAC 
CAACGGACCA ATGACCCTTG CCTTGGAAAT AAATGCGTAC ATGGCACCTG CTTGCCCATC 
AATGCGTTCT CCTACAGCTG TAAGTGCTTG GAGGGCCATG GAGGTGTCCT CTGTGATGAA 
GAGGAGGATC TGTTTAACCC ATGCCAGGCG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCAGGTCTGG GGCAGCCCTA CTGTGAATGC AGCAGTGGAT ACACGGGGGA CAGCTGTGAT 
CGAGAAATCT CTTGTCGAGG GGAAAGGATA AGAGATTATT ACCAAAAGCA GCAGGGCTAT 
GCTGCTTGCC AAACAACCAA GAAGGTGTCC CGATTAGAGT GCAGAGGTGG GTGTGCAGGA 
GGGCAGTGCT GTGGACCGCT GAGGAGCAAG CGGCGGAAAT ACTCTTTCGA ATGCACTGAC 
GGCTCCTCCT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGTAC GAGGTGTGTG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276065.1
CDS	1912..6477
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	NM_001289136.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289136.3

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGTAGTGTT TTGCACTGCC CTGCCGCCTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTTCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC ACAATCAAAG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG ACGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTTGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CCAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTCCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTCCACAA CTTGAACCTT 
CTCTCCCTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTTCGG 
GCCATTCAAA CTATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCATAC CAACCCGATT GAGACCAGTG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CGGATCTGGC TTGCCCTGAA 
AAGTGTCGCT GTGAAGGAAC CACAGTAGAT TGCTCTAATC AAAAGCTCAA CAAAATCCCG 
GAGCACATTC CCCAGTACAC TGCAGAGTTG CGTCTCAATA ATAATGAATT TACCGTGTTG 
GAAGCCACAG GAATCTTTAA GAAACTTCCT CAATTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ATATTGAGGA GGGAGCATTT GAAGGAGCAT CTGGTGTAAA TGAAATACTT 
CTTACGAGTA ATCGTTTGGA AAATGTGCAG CATAAGATGT TCAAGGGATT GGAAAGCCTC 
AAAACTTTGA TGTTGAGAAG CAATCGAATA ACCTGTGTGG GGAATGACAG TTTCATAGGA 
CTCAGTTCTG TGCGTTTGCT TTCTTTGTAT GATAATCAAA TTACTACAGT TGCACCAGGG 
GCATTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTTAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAGTGG CTGAGAAAGA AGAGAATTGT CACGGGAAAT 
CCTAGATGTC AAAAACCATA CTTCCTGAAA GAAATACCCA TCCAGGATGT GGCCATTCAG 
GACTTCACTT GTGATGACGG AAATGATGAC AATAGTTGCT CCCCACTTTC TCGCTGTCCT 
ACTGAATGTA CTTGCTTGGA TACAGTCGTC CGATGTAGCA ACAAGGGTTT GAAGGTCTTG 
CCGAAAGGTA TTCCAAGAGA TGTCACAGAG TTGTATCTGG ATGGAAACCA ATTTACACTG 
GTTCCCAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACGC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CTTAATTCTT 
AGTTACAACC GTCTGAGATG TATTCCTCCT CGCACCTTTG ATGGATTAAA GTCTCTTCGA 
TTACTTTCTC TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
TCTGCATTAT CACATCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAGCCTG GAATTGCTCG TTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTTTTACTC ACAACTCCCT CCAAAAAATT TACCTGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAG TGTAACCCCT GCCTATCAAA TCCGTGTAAA 
AATGATGGCA CATGTAATAG TGATCCAGTT GACTTTTACC GATGCACCTG TCCATATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATT CATGCCTGCA TCAGTAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA GGAAGGAGAA GAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAC GTTGATGATT GTGAAGATAA TGACTGTGAA 
AATAATTCTA CATGTGTCGA TGGCATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAT 
ACAGGTGAGT TGTGTGAGGA GAAGCTGGAC TTCTGTGCCC AGGACCTGAA CCCCTGCCAG 
CACGATTCAA AGTGCATCCT AACTCCAAAG GGATTCAAAT GTGACTGCAC ACCAGGGTAC 
GTAGGTGAAC ACTGCGACAT CGATTTTGAC GACTGCCAAG ACAACAAGTG TAAAAACGGA 
GCCCACTGCA CAGATGCAGT GAACGGCTAT ACGTGCATAT GCCCCGAAGG TTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA GTGTATCGTC AGAATAAATG AGCCAATATG TCAGTGTTTG 
CCTGGCTATC AGGGAGAAAA GTGTGAAAAA TTGGTTAGTG TGAATTTTAT AAACAAAGAG 
TCTTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CGAACATAAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG AATCCTCCTG TATAAGGGTG ACAAAGACCA TATCGCGGTA 
GAACTCTATC GGGGGCGTGT TCGTGCCAGC TATGACACCG GCTCTCATCC AGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAACT ACTTGCCTTG 
GATCAGAGTC TCTCTTTGTC CGTGGATGGT GGGAACCCCA AAATCATCAC TAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC AGGGAAGAGT 
AACGTGGCAT CTCTGCGCCA GGCCCCTGGG CAGAACGGAA CCAGCTTCCA CGGCTGCATC 
CGGAACCTTT ACATCAACAG TGAGCTGCAG GACTTCCAGA AGGTGCCGAT GCAAACAGGC 
ATTTTGCCTG GCTGTGAGCC ATGCCACAAG AAGGTGTGTG CCCATGGCAC ATGCCAGCCC 
AGCAGCCAGG CAGGCTTCAC CTGCGAGTGC CAGGAAGGAT GGATGGGGCC CCTCTGTGAC 
CAACGGACCA ATGACCCTTG CCTTGGAAAT AAATGCGTAC ATGGCACCTG CTTGCCCATC 
AATGCGTTCT CCTACAGCTG TAAGTGCTTG GAGGGCCATG GAGGTGTCCT CTGTGATGAA 
GAGGAGGATC TGTTTAACCC ATGCCAGGCG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCAGGTCTGG GGCAGCCCTA CTGTGAATGC AGCAGTGGAT ACACGGGGGA CAGCTGTGAT 
CGAGAAATCT CTTGTCGAGG GGAAAGGATA AGAGATTATT ACCAAAAGCA GCAGGGCTAT 
GCTGCTTGCC AAACAACCAA GAAGGTGTCC CGATTAGAGT GCAGAGGTGG GTGTGCAGGA 
GGGCAGTGCT GTGGACCGCT GAGGAGCAAG CGGCGGAAAT ACTCTTTCGA ATGCACTGAC 
GGCTCCTCCT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGTAC GAGGTGTGTG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005248211.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248268.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu38497
Clone ID Related Accession (Same CDS sequence)	XM_005248211.3
Accession Version	XM_005248211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCGGA TCTGGCTTGC CCTGAAAAGT GTCGCTGTGA AGGAACCACA 
GTAGATTGCT CTAATCAAAA GCTCAACAAA ATCCCGGAGC ACATTCCCCA GTACACTGCA 
GAGTTGCGTC TCAATAATAA TGAATTTACC GTGTTGGAAG CCACAGGAAT CTTTAAGAAA 
CTTCCTCAAT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGATAT TGAGGAGGGA 
GCATTTGAAG GAGCATCTGG TGTAAATGAA ATACTTCTTA CGAGTAATCG TTTGGAAAAT 
GTGCAGCATA AGATGTTCAA GGGATTGGAA AGCCTCAAAA CTTTGATGTT GAGAAGCAAT 
CGAATAACCT GTGTGGGGAA TGACAGTTTC ATAGGACTCA GTTCTGTGCG TTTGCTTTCT 
TTGTATGATA ATCAAATTAC TACAGTTGCA CCAGGGGCAT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTT AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAGTGGCTGA GAAAGAAGAG AATTGTCACG GGAAATCCTA GATGTCAAAA ACCATACTTC 
CTGAAAGAAA TACCCATCCA GGATGTGGCC ATTCAGGACT TCACTTGTGA TGACGGAAAT 
GATGACAATA GTTGCTCCCC ACTTTCTCGC TGTCCTACTG AATGTACTTG CTTGGATACA 
GTCGTCCGAT GTAGCAACAA GGGTTTGAAG GTCTTGCCGA AAGGTATTCC AAGAGATGTC 
ACAGAGTTGT ATCTGGATGG AAACCAATTT ACACTGGTTC CCAAGGAACT CTCCAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACGCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCTTA ATTCTTAGTT ACAACCGTCT GAGATGTATT 
CCTCCTCGCA CCTTTGATGG ATTAAAGTCT CTTCGATTAC TTTCTCTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAAT GATCTTTCTG CATTATCACA TCTAGCAATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AGCCTGGAAT TGCTCGTTGT GCTGGTCCTG GAGAAATGGC AGATAAACTT 
TTACTCACAA CTCCCTCCAA AAAATTTACC TGTCAAGGTC CTGTGGATGT CAATATTCTA 
GCTAAGTGTA ACCCCTGCCT ATCAAATCCG TGTAAAAATG ATGGCACATG TAATAGTGAT 
CCAGTTGACT TTTACCGATG CACCTGTCCA TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATTCATG CCTGCATCAG TAACCCATGT AAACATGGAG GAACTTGCCA CTTAAAGGAA 
GGAGAAGAAG ATGGATTCTG GTGTATTTGT GCTGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAACGTTG ATGATTGTGA AGATAATGAC TGTGAAAATA ATTCTACATG TGTCGATGGC 
ATTAATAACT ACACATGCCT TTGCCCACCT GAGTATACAG GTGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GTGCCCAGGA CCTGAACCCC TGCCAGCACG ATTCAAAGTG CATCCTAACT 
CCAAAGGGAT TCAAATGTGA CTGCACACCA GGGTACGTAG GTGAACACTG CGACATCGAT 
TTTGACGACT GCCAAGACAA CAAGTGTAAA AACGGAGCCC ACTGCACAGA TGCAGTGAAC 
GGCTATACGT GCATATGCCC CGAAGGTTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGTACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCTCAGTGT 
ATCGTCAGAA TAAATGAGCC AATATGTCAG TGTTTGCCTG GCTATCAGGG AGAAAAGTGT 
GAAAAATTGG TTAGTGTGAA TTTTATAAAC AAAGAGTCTT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACGAA CATAACACTT CAGATTGCCA CAGATGAAGA CAGCGGAATC 
CTCCTGTATA AGGGTGACAA AGACCATATC GCGGTAGAAC TCTATCGGGG GCGTGTTCGT 
GCCAGCTATG ACACCGGCTC TCATCCAGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAACTACTT GCCTTGGATC AGAGTCTCTC TTTGTCCGTG 
GATGGTGGGA ACCCCAAAAT CATCACTAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCAGGG AAGAGTAACG TGGCATCTCT GCGCCAGGCC 
CCTGGGCAGA ACGGAACCAG CTTCCACGGC TGCATCCGGA ACCTTTACAT CAACAGTGAG 
CTGCAGGACT TCCAGAAGGT GCCGATGCAA ACAGGCATTT TGCCTGGCTG TGAGCCATGC 
CACAAGAAGG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAGGCAGG CTTCACCTGC 
GAGTGCCAGG AAGGATGGAT GGGGCCCCTC TGTGACCAAC GGACCAATGA CCCTTGCCTT 
GGAAATAAAT GCGTACATGG CACCTGCTTG CCCATCAATG CGTTCTCCTA CAGCTGTAAG 
TGCTTGGAGG GCCATGGAGG TGTCCTCTGT GATGAAGAGG AGGATCTGTT TAACCCATGC 
CAGGCGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCAG GTCTGGGGCA GCCCTACTGT 
GAATGCAGCA GTGGATACAC GGGGGACAGC TGTGATCGAG AAATCTCTTG TCGAGGGGAA 
AGGATAAGAG ATTATTACCA AAAGCAGCAG GGCTATGCTG CTTGCCAAAC AACCAAGAAG 
GTGTCCCGAT TAGAGTGCAG AGGTGGGTGT GCAGGAGGGC AGTGCTGTGG ACCGCTGAGG 
AGCAAGCGGC GGAAATACTC TTTCGAATGC ACTGACGGCT CCTCCTTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGTACGAGG TGTGTGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248268.1
CDS	1912..6513
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	XM_005248211.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248211.3

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCGGA TCTGGCTTGC CCTGAAAAGT GTCGCTGTGA AGGAACCACA 
GTAGATTGCT CTAATCAAAA GCTCAACAAA ATCCCGGAGC ACATTCCCCA GTACACTGCA 
GAGTTGCGTC TCAATAATAA TGAATTTACC GTGTTGGAAG CCACAGGAAT CTTTAAGAAA 
CTTCCTCAAT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGATAT TGAGGAGGGA 
GCATTTGAAG GAGCATCTGG TGTAAATGAA ATACTTCTTA CGAGTAATCG TTTGGAAAAT 
GTGCAGCATA AGATGTTCAA GGGATTGGAA AGCCTCAAAA CTTTGATGTT GAGAAGCAAT 
CGAATAACCT GTGTGGGGAA TGACAGTTTC ATAGGACTCA GTTCTGTGCG TTTGCTTTCT 
TTGTATGATA ATCAAATTAC TACAGTTGCA CCAGGGGCAT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTT AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAGTGGCTGA GAAAGAAGAG AATTGTCACG GGAAATCCTA GATGTCAAAA ACCATACTTC 
CTGAAAGAAA TACCCATCCA GGATGTGGCC ATTCAGGACT TCACTTGTGA TGACGGAAAT 
GATGACAATA GTTGCTCCCC ACTTTCTCGC TGTCCTACTG AATGTACTTG CTTGGATACA 
GTCGTCCGAT GTAGCAACAA GGGTTTGAAG GTCTTGCCGA AAGGTATTCC AAGAGATGTC 
ACAGAGTTGT ATCTGGATGG AAACCAATTT ACACTGGTTC CCAAGGAACT CTCCAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACGCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCTTA ATTCTTAGTT ACAACCGTCT GAGATGTATT 
CCTCCTCGCA CCTTTGATGG ATTAAAGTCT CTTCGATTAC TTTCTCTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAAT GATCTTTCTG CATTATCACA TCTAGCAATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AGCCTGGAAT TGCTCGTTGT GCTGGTCCTG GAGAAATGGC AGATAAACTT 
TTACTCACAA CTCCCTCCAA AAAATTTACC TGTCAAGGTC CTGTGGATGT CAATATTCTA 
GCTAAGTGTA ACCCCTGCCT ATCAAATCCG TGTAAAAATG ATGGCACATG TAATAGTGAT 
CCAGTTGACT TTTACCGATG CACCTGTCCA TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATTCATG CCTGCATCAG TAACCCATGT AAACATGGAG GAACTTGCCA CTTAAAGGAA 
GGAGAAGAAG ATGGATTCTG GTGTATTTGT GCTGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAACGTTG ATGATTGTGA AGATAATGAC TGTGAAAATA ATTCTACATG TGTCGATGGC 
ATTAATAACT ACACATGCCT TTGCCCACCT GAGTATACAG GTGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GTGCCCAGGA CCTGAACCCC TGCCAGCACG ATTCAAAGTG CATCCTAACT 
CCAAAGGGAT TCAAATGTGA CTGCACACCA GGGTACGTAG GTGAACACTG CGACATCGAT 
TTTGACGACT GCCAAGACAA CAAGTGTAAA AACGGAGCCC ACTGCACAGA TGCAGTGAAC 
GGCTATACGT GCATATGCCC CGAAGGTTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGTACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCTCAGTGT 
ATCGTCAGAA TAAATGAGCC AATATGTCAG TGTTTGCCTG GCTATCAGGG AGAAAAGTGT 
GAAAAATTGG TTAGTGTGAA TTTTATAAAC AAAGAGTCTT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACGAA CATAACACTT CAGATTGCCA CAGATGAAGA CAGCGGAATC 
CTCCTGTATA AGGGTGACAA AGACCATATC GCGGTAGAAC TCTATCGGGG GCGTGTTCGT 
GCCAGCTATG ACACCGGCTC TCATCCAGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAACTACTT GCCTTGGATC AGAGTCTCTC TTTGTCCGTG 
GATGGTGGGA ACCCCAAAAT CATCACTAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCAGGG AAGAGTAACG TGGCATCTCT GCGCCAGGCC 
CCTGGGCAGA ACGGAACCAG CTTCCACGGC TGCATCCGGA ACCTTTACAT CAACAGTGAG 
CTGCAGGACT TCCAGAAGGT GCCGATGCAA ACAGGCATTT TGCCTGGCTG TGAGCCATGC 
CACAAGAAGG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAGGCAGG CTTCACCTGC 
GAGTGCCAGG AAGGATGGAT GGGGCCCCTC TGTGACCAAC GGACCAATGA CCCTTGCCTT 
GGAAATAAAT GCGTACATGG CACCTGCTTG CCCATCAATG CGTTCTCCTA CAGCTGTAAG 
TGCTTGGAGG GCCATGGAGG TGTCCTCTGT GATGAAGAGG AGGATCTGTT TAACCCATGC 
CAGGCGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCAG GTCTGGGGCA GCCCTACTGT 
GAATGCAGCA GTGGATACAC GGGGGACAGC TGTGATCGAG AAATCTCTTG TCGAGGGGAA 
AGGATAAGAG ATTATTACCA AAAGCAGCAG GGCTATGCTG CTTGCCAAAC AACCAAGAAG 
GTGTCCCGAT TAGAGTGCAG AGGTGGGTGT GCAGGAGGGC AGTGCTGTGG ACCGCTGAGG 
AGCAAGCGGC GGAAATACTC TTTCGAATGC ACTGACGGCT CCTCCTTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGTACGAGG TGTGTGTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_006713986.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714049.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu38498
Clone ID Related Accession (Same CDS sequence)	XM_006713986.3
Accession Version	XM_006713986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCGGA TCTGGCTTGC CCTGAAAAGT GTCGCTGTGA AGGAACCACA 
GTAGATTGCT CTAATCAAAA GCTCAACAAA ATCCCGGAGC ACATTCCCCA GTACACTGCA 
GAGTTGCGTC TCAATAATAA TGAATTTACC GTGTTGGAAG CCACAGGAAT CTTTAAGAAA 
CTTCCTCAAT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGATAT TGAGGAGGGA 
GCATTTGAAG GAGCATCTGG TGTAAATGAA ATACTTCTTA CGAGTAATCG TTTGGAAAAT 
GTGCAGCATA AGATGTTCAA GGGATTGGAA AGCCTCAAAA CTTTGATGTT GAGAAGCAAT 
CGAATAACCT GTGTGGGGAA TGACAGTTTC ATAGGACTCA GTTCTGTGCG TTTGCTTTCT 
TTGTATGATA ATCAAATTAC TACAGTTGCA CCAGGGGCAT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTT AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAGTGGCTGA GAAAGAAGAG AATTGTCACG GGAAATCCTA GATGTCAAAA ACCATACTTC 
CTGAAAGAAA TACCCATCCA GGATGTGGCC ATTCAGGACT TCACTTGTGA TGACGGAAAT 
GATGACAATA GTTGCTCCCC ACTTTCTCGC TGTCCTACTG AATGTACTTG CTTGGATACA 
GTCGTCCGAT GTAGCAACAA GGGTTTGAAG GTCTTGCCGA AAGGTATTCC AAGAGATGTC 
ACAGAGTTGT ATCTGGATGG AAACCAATTT ACACTGGTTC CCAAGGAACT CTCCAACTAC 
AAACATTTAA CACTTATAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714049.1
CDS	1912..6441
Translation	MRGVGWQMLSLSLGLVLAILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	XM_006713986.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713986.3

ORF Insert Sequence:

ATGCGCGGCG TTGGCTGGCA GATGCTGTCC CTGTCGCTGG GGTTAGTGCT GGCGATCCTG 
AACAAGGTGG CACCGCAGGC GTGCCCGGCG CAGTGCTCTT GCTCGGGCAG CACAGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTAAATG GAAATAACAT CACAAGAATT ACGAAGACAG ATTTTGCTGG TCTTAGACAT 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTAGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTGC GAAGCTATAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATCCCAA GGAAAGCTTT CCGTGGGGCA GTTGACATAA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTGCA TTCAAACAAC CTGTATTGTG ACTGCCACCT GGCCTGGCTC 
TCCGACTGGC TTCGCCAAAG GCCTCGGGTT GGTCTGTACA CTCAGTGTAT GGGCCCCTCC 
CACCTGAGAG GCCATAATGT AGCCGAGGTT CAAAAACGAG AATTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGTAGTG TTTTGCACTG CCCTGCCGCC 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTTCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACACAATCAA AGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGACGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTTG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCCAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTC 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTCCAC 
AACTTGAACC TTCTCTCCCT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTTC GGGCCATTCA AACTATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAT ACCAACCCGA TTGAGACCAG TGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCGGA TCTGGCTTGC CCTGAAAAGT GTCGCTGTGA AGGAACCACA 
GTAGATTGCT CTAATCAAAA GCTCAACAAA ATCCCGGAGC ACATTCCCCA GTACACTGCA 
GAGTTGCGTC TCAATAATAA TGAATTTACC GTGTTGGAAG CCACAGGAAT CTTTAAGAAA 
CTTCCTCAAT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGATAT TGAGGAGGGA 
GCATTTGAAG GAGCATCTGG TGTAAATGAA ATACTTCTTA CGAGTAATCG TTTGGAAAAT 
GTGCAGCATA AGATGTTCAA GGGATTGGAA AGCCTCAAAA CTTTGATGTT GAGAAGCAAT 
CGAATAACCT GTGTGGGGAA TGACAGTTTC ATAGGACTCA GTTCTGTGCG TTTGCTTTCT 
TTGTATGATA ATCAAATTAC TACAGTTGCA CCAGGGGCAT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTT AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAGTGGCTGA GAAAGAAGAG AATTGTCACG GGAAATCCTA GATGTCAAAA ACCATACTTC 
CTGAAAGAAA TACCCATCCA GGATGTGGCC ATTCAGGACT TCACTTGTGA TGACGGAAAT 
GATGACAATA GTTGCTCCCC ACTTTCTCGC TGTCCTACTG AATGTACTTG CTTGGATACA 
GTCGTCCGAT GTAGCAACAA GGGTTTGAAG GTCTTGCCGA AAGGTATTCC AAGAGATGTC 
ACAGAGTTGT ATCTGGATGG AAACCAATTT ACACTGGTTC CCAAGGAACT CTCCAACTAC 
AAACATTTAA CACTTATAAT TCTTAGTTAC AACCGTCTGA GATGTATTCC TCCTCGCACC 
TTTGATGGAT TAAAGTCTCT TCGATTACTT TCTCTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTTCTGCA TTATCACATC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAG 
CCTGGAATTG CTCGTTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTTTT ACTCACAACT 
CCCTCCAAAA AATTTACCTG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAGTGTAAC 
CCCTGCCTAT CAAATCCGTG TAAAAATGAT GGCACATGTA ATAGTGATCC AGTTGACTTT 
TACCGATGCA CCTGTCCATA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATTCATGCC 
TGCATCAGTA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAGGAAGG AGAAGAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAGT CAACGTTGAT 
GATTGTGAAG ATAATGACTG TGAAAATAAT TCTACATGTG TCGATGGCAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTATACAGGT GAGTTGTGTG AGGAGAAGCT GGACTTCTGT 
GCCCAGGACC TGAACCCCTG CCAGCACGAT TCAAAGTGCA TCCTAACTCC AAAGGGATTC 
AAATGTGACT GCACACCAGG GTACGTAGGT GAACACTGCG ACATCGATTT TGACGACTGC 
CAAGACAACA AGTGTAAAAA CGGAGCCCAC TGCACAGATG CAGTGAACGG CTATACGTGC 
ATATGCCCCG AAGGTTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAGTGTAT CGTCAGAATA 
AATGAGCCAA TATGTCAGTG TTTGCCTGGC TATCAGGGAG AAAAGTGTGA AAAATTGGTT 
AGTGTGAATT TTATAAACAA AGAGTCTTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACGAACA TAACACTTCA GATTGCCACA GATGAAGACA GCGGAATCCT CCTGTATAAG 
GGTGACAAAG ACCATATCGC GGTAGAACTC TATCGGGGGC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCTC ATCCAGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AACTACTTGC CTTGGATCAG AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC 
CCCAAAATCA TCACTAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCAGGGAA GAGTAACGTG GCATCTCTGC GCCAGGCCCC TGGGCAGAAC 
GGAACCAGCT TCCACGGCTG CATCCGGAAC CTTTACATCA ACAGTGAGCT GCAGGACTTC 
CAGAAGGTGC CGATGCAAAC AGGCATTTTG CCTGGCTGTG AGCCATGCCA CAAGAAGGTG 
TGTGCCCATG GCACATGCCA GCCCAGCAGC CAGGCAGGCT TCACCTGCGA GTGCCAGGAA 
GGATGGATGG GGCCCCTCTG TGACCAACGG ACCAATGACC CTTGCCTTGG AAATAAATGC 
GTACATGGCA CCTGCTTGCC CATCAATGCG TTCTCCTACA GCTGTAAGTG CTTGGAGGGC 
CATGGAGGTG TCCTCTGTGA TGAAGAGGAG GATCTGTTTA ACCCATGCCA GGCGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCAGGT CTGGGGCAGC CCTACTGTGA ATGCAGCAGT 
GGATACACGG GGGACAGCTG TGATCGAGAA ATCTCTTGTC GAGGGGAAAG GATAAGAGAT 
TATTACCAAA AGCAGCAGGG CTATGCTGCT TGCCAAACAA CCAAGAAGGT GTCCCGATTA 
GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG 
AAATACTCTT TCGAATGCAC TGACGGCTCC TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_011513909.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512211.1 Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

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CloneID	OHu62720
Clone ID Related Accession (Same CDS sequence)	XM_011513909.2
Accession Version	XM_011513909.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513909.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG CAAGTGAGAC GCCACTTTGT TCTCCAGAGT CCATAAACGG AGTCACCTTG 
CGATTGCCAG CATCCAGGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT 
TTTGCTGGTC TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT 
GAAAGAGGAG CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC 
CTTCAGCTGT TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC 
AGTGAAAACC AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA 
AATTTGCAAC TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC 
CGGGACCTGG AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT 
TTCAACCATA TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC 
TGCCACCTGG CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT 
CAGTGTATGG GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA 
TTTGTCTGCA GTGATGAGGA AGAAGGTCAC CAGTCATTTA TGGCTCCTTC TTGTAGTGTT 
TTGCACTGCC CTGCCGCCTG TACCTGTAGC AACAATATCG TAGACTGTCG TGGGAAAGGT 
CTCACTGAGA TCCCCACAAA TCTTCCAGAG ACCATCACAG AAATACGTTT GGAACAGAAC 
ACAATCAAAG TCATCCCTCC TGGAGCTTTC TCACCATATA AAAAGCTTAG ACGAATTGAC 
CTGAGCAATA ATCAGATCTC TGAACTTGCA CCAGATGCTT TCCAAGGACT ACGCTCTCTG 
AATTCACTTG TCCTCTATGG AAATAAAATC ACAGAACTCC CCAAAAGTTT ATTTGAAGGA 
CTGTTTTCCT TACAGCTCCT ATTATTGAAT GCCAACAAGA TAAACTGCCT TCGGGTAGAT 
GCTTTTCAGG ATCTCCACAA CTTGAACCTT CTCTCCCTAT ATGACAACAA GCTTCAGACC 
ATCGCCAAGG GGACCTTTTC ACCTCTTCGG GCCATTCAAA CTATGCATTT GGCCCAGAAC 
CCCTTTATTT GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCATAC CAACCCGATT 
GAGACCAGTG GTGCCCGTTG CACCAGCCCC CGCCGCCTGG CAAACAAAAG AATTGGACAG 
ATCAAAAGCA AGAAATTCCG TTGTTCAGCT AAAGAACAGT ATTTCATTCC AGGTACAGAA 
GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCGGATC TGGCTTGCCC TGAAAAGTGT 
CGCTGTGAAG GAACCACAGT AGATTGCTCT AATCAAAAGC TCAACAAAAT CCCGGAGCAC 
ATTCCCCAGT ACACTGCAGA GTTGCGTCTC AATAATAATG AATTTACCGT GTTGGAAGCC 
ACAGGAATCT TTAAGAAACT TCCTCAATTA CGTAAAATAA ACTTTAGCAA CAATAAGATC 
ACAGATATTG AGGAGGGAGC ATTTGAAGGA GCATCTGGTG TAAATGAAAT ACTTCTTACG 
AGTAATCGTT TGGAAAATGT GCAGCATAAG ATGTTCAAGG GATTGGAAAG CCTCAAAACT 
TTGATGTTGA GAAGCAATCG AATAACCTGT GTGGGGAATG ACAGTTTCAT AGGACTCAGT 
TCTGTGCGTT TGCTTTCTTT GTATGATAAT CAAATTACTA CAGTTGCACC AGGGGCATTT 
GATACTCTCC ATTCTTTATC TACTCTAAAC CTCTTGGCCA ATCCTTTTAA CTGTAACTGC 
TACCTGGCTT GGTTGGGAGA GTGGCTGAGA AAGAAGAGAA TTGTCACGGG AAATCCTAGA 
TGTCAAAAAC CATACTTCCT GAAAGAAATA CCCATCCAGG ATGTGGCCAT TCAGGACTTC 
ACTTGTGATG ACGGAAATGA TGACAATAGT TGCTCCCCAC TTTCTCGCTG TCCTACTGAA 
TGTACTTGCT TGGATACAGT CGTCCGATGT AGCAACAAGG GTTTGAAGGT CTTGCCGAAA 
GGTATTCCAA GAGATGTCAC AGAGTTGTAT CTGGATGGAA ACCAATTTAC ACTGGTTCCC 
AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC 
ACGCTTTCTA ATCAGAGCTT CAGCAACATG ACCCAGCTCC TCACCTTAAT TCTTAGTTAC 
AACCGTCTGA GATGTATTCC TCCTCGCACC TTTGATGGAT TAAAGTCTCT TCGATTACTT 
TCTCTACATG GAAATGACAT TTCTGTTGTG CCTGAAGGTG CTTTCAATGA TCTTTCTGCA 
TTATCACATC TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA 
TCCGACTGGG TGAAGTCGGA ATATAAGGAG CCTGGAATTG CTCGTTGTGC TGGTCCTGGA 
GAAATGGCAG ATAAACTTTT ACTCACAACT CCCTCCAAAA AATTTACCTG TCAAGGTCCT 
GTGGATGTCA ATATTCTAGC TAAGTGTAAC CCCTGCCTAT CAAATCCGTG TAAAAATGAT 
GGCACATGTA ATAGTGATCC AGTTGACTTT TACCGATGCA CCTGTCCATA TGGTTTCAAG 
GGGCAGGACT GTGATGTCCC AATTCATGCC TGCATCAGTA ACCCATGTAA ACATGGAGGA 
ACTTGCCACT TAAAGGAAGG AGAAGAAGAT GGATTCTGGT GTATTTGTGC TGATGGATTT 
GAAGGAGAAA ATTGTGAAGT CAACGTTGAT GATTGTGAAG ATAATGACTG TGAAAATAAT 
TCTACATGTG TCGATGGCAT TAATAACTAC ACATGCCTTT GCCCACCTGA GTATACAGGT 
GAGTTGTGTG AGGAGAAGCT GGACTTCTGT GCCCAGGACC TGAACCCCTG CCAGCACGAT 
TCAAAGTGCA TCCTAACTCC AAAGGGATTC AAATGTGACT GCACACCAGG GTACGTAGGT 
GAACACTGCG ACATCGATTT TGACGACTGC CAAGACAACA AGTGTAAAAA CGGAGCCCAC 
TGCACAGATG CAGTGAACGG CTATACGTGC ATATGCCCCG AAGGTTACAG TGGCTTGTTC 
TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGTACCAGCC CCTGTGATAA TTTTGATTGT 
CAGAATGGAG CTCAGTGTAT CGTCAGAATA AATGAGCCAA TATGTCAGTG TTTGCCTGGC 
TATCAGGGAG AAAAGTGTGA AAAATTGGTT AGTGTGAATT TTATAAACAA AGAGTCTTAT 
CTTCAGATTC CTTCAGCCAA GGTTCGGCCT CAGACGAACA TAACACTTCA GATTGCCACA 
GATGAAGACA GCGGAATCCT CCTGTATAAG GGTGACAAAG ACCATATCGC GGTAGAACTC 
TATCGGGGGC GTGTTCGTGC CAGCTATGAC ACCGGCTCTC ATCCAGCTTC TGCCATTTAC 
AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTGG AACTACTTGC CTTGGATCAG 
AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC CCCAAAATCA TCACTAACTT GTCAAAGCAG 
TCCACTCTGA ATTTTGACTC TCCACTCTAT GTAGGAGGCA TGCCAGGGAA GAGTAACGTG 
GCATCTCTGC GCCAGGCCCC TGGGCAGAAC GGAACCAGCT TCCACGGCTG CATCCGGAAC 
CTTTACATCA ACAGTGAGCT GCAGGACTTC CAGAAGGTGC CGATGCAAAC AGGCATTTTG 
CCTGGCTGTG AGCCATGCCA CAAGAAGGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC 
CAGGCAGGCT TCACCTGCGA GTGCCAGGAA GGATGGATGG GGCCCCTCTG TGACCAACGG 
ACCAATGACC CTTGCCTTGG AAATAAATGC GTACATGGCA CCTGCTTGCC CATCAATGCG 
TTCTCCTACA GCTGTAAGTG CTTGGAGGGC CATGGAGGTG TCCTCTGTGA TGAAGAGGAG 
GATCTGTTTA ACCCATGCCA GGCGATCAAG TGCAAGCATG GGAAGTGCAG GCTTTCAGGT 
CTGGGGCAGC CCTACTGTGA ATGCAGCAGT GGATACACGG GGGACAGCTG TGATCGAGAA 
ATCTCTTGTC GAGGGGAAAG GATAAGAGAT TATTACCAAA AGCAGCAGGG CTATGCTGCT 
TGCCAAACAA CCAAGAAGGT GTCCCGATTA GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG 
TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG AAATACTCTT TCGAATGCAC TGACGGCTCC 
TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512211.1
CDS	42..4541
Translation	MPGASETPLCSPESINGVTLRLPASRDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRINEPICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITNLSKQSTLNFDSPLYVGGMPGKSNVASLRQAPGQNGTSFHGCIRNLYINSELQDFQKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCVS

Target ORF information:

RefSeq Version	XM_011513909.2
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513909.2

ORF Insert Sequence:

ATGCCCGGGG CAAGTGAGAC GCCACTTTGT TCTCCAGAGT CCATAAACGG AGTCACCTTG 
CGATTGCCAG CATCCAGGGA TTTAAATGGA AATAACATCA CAAGAATTAC GAAGACAGAT 
TTTGCTGGTC TTAGACATCT AAGAGTTCTT CAGCTTATGG AGAATAAGAT TAGCACCATT 
GAAAGAGGAG CATTCCAGGA TCTTAAAGAA CTAGAGAGAC TGCGTTTAAA CAGAAATCAC 
CTTCAGCTGT TTCCTGAGTT GCTGTTTCTT GGGACTGCGA AGCTATACAG GCTTGATCTC 
AGTGAAAACC AAATTCAGGC AATCCCAAGG AAAGCTTTCC GTGGGGCAGT TGACATAAAA 
AATTTGCAAC TGGATTACAA CCAGATCAGC TGTATTGAAG ATGGGGCATT CAGGGCTCTC 
CGGGACCTGG AAGTGCTCAC TCTCAACAAT AACAACATTA CTAGACTTTC TGTGGCAAGT 
TTCAACCATA TGCCTAAACT TAGGACTTTT CGACTGCATT CAAACAACCT GTATTGTGAC 
TGCCACCTGG CCTGGCTCTC CGACTGGCTT CGCCAAAGGC CTCGGGTTGG TCTGTACACT 
CAGTGTATGG GCCCCTCCCA CCTGAGAGGC CATAATGTAG CCGAGGTTCA AAAACGAGAA 
TTTGTCTGCA GTGATGAGGA AGAAGGTCAC CAGTCATTTA TGGCTCCTTC TTGTAGTGTT 
TTGCACTGCC CTGCCGCCTG TACCTGTAGC AACAATATCG TAGACTGTCG TGGGAAAGGT 
CTCACTGAGA TCCCCACAAA TCTTCCAGAG ACCATCACAG AAATACGTTT GGAACAGAAC 
ACAATCAAAG TCATCCCTCC TGGAGCTTTC TCACCATATA AAAAGCTTAG ACGAATTGAC 
CTGAGCAATA ATCAGATCTC TGAACTTGCA CCAGATGCTT TCCAAGGACT ACGCTCTCTG 
AATTCACTTG TCCTCTATGG AAATAAAATC ACAGAACTCC CCAAAAGTTT ATTTGAAGGA 
CTGTTTTCCT TACAGCTCCT ATTATTGAAT GCCAACAAGA TAAACTGCCT TCGGGTAGAT 
GCTTTTCAGG ATCTCCACAA CTTGAACCTT CTCTCCCTAT ATGACAACAA GCTTCAGACC 
ATCGCCAAGG GGACCTTTTC ACCTCTTCGG GCCATTCAAA CTATGCATTT GGCCCAGAAC 
CCCTTTATTT GTGACTGCCA TCTCAAGTGG CTAGCGGATT ATCTCCATAC CAACCCGATT 
GAGACCAGTG GTGCCCGTTG CACCAGCCCC CGCCGCCTGG CAAACAAAAG AATTGGACAG 
ATCAAAAGCA AGAAATTCCG TTGTTCAGCT AAAGAACAGT ATTTCATTCC AGGTACAGAA 
GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCGGATC TGGCTTGCCC TGAAAAGTGT 
CGCTGTGAAG GAACCACAGT AGATTGCTCT AATCAAAAGC TCAACAAAAT CCCGGAGCAC 
ATTCCCCAGT ACACTGCAGA GTTGCGTCTC AATAATAATG AATTTACCGT GTTGGAAGCC 
ACAGGAATCT TTAAGAAACT TCCTCAATTA CGTAAAATAA ACTTTAGCAA CAATAAGATC 
ACAGATATTG AGGAGGGAGC ATTTGAAGGA GCATCTGGTG TAAATGAAAT ACTTCTTACG 
AGTAATCGTT TGGAAAATGT GCAGCATAAG ATGTTCAAGG GATTGGAAAG CCTCAAAACT 
TTGATGTTGA GAAGCAATCG AATAACCTGT GTGGGGAATG ACAGTTTCAT AGGACTCAGT 
TCTGTGCGTT TGCTTTCTTT GTATGATAAT CAAATTACTA CAGTTGCACC AGGGGCATTT 
GATACTCTCC ATTCTTTATC TACTCTAAAC CTCTTGGCCA ATCCTTTTAA CTGTAACTGC 
TACCTGGCTT GGTTGGGAGA GTGGCTGAGA AAGAAGAGAA TTGTCACGGG AAATCCTAGA 
TGTCAAAAAC CATACTTCCT GAAAGAAATA CCCATCCAGG ATGTGGCCAT TCAGGACTTC 
ACTTGTGATG ACGGAAATGA TGACAATAGT TGCTCCCCAC TTTCTCGCTG TCCTACTGAA 
TGTACTTGCT TGGATACAGT CGTCCGATGT AGCAACAAGG GTTTGAAGGT CTTGCCGAAA 
GGTATTCCAA GAGATGTCAC AGAGTTGTAT CTGGATGGAA ACCAATTTAC ACTGGTTCCC 
AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC 
ACGCTTTCTA ATCAGAGCTT CAGCAACATG ACCCAGCTCC TCACCTTAAT TCTTAGTTAC 
AACCGTCTGA GATGTATTCC TCCTCGCACC TTTGATGGAT TAAAGTCTCT TCGATTACTT 
TCTCTACATG GAAATGACAT TTCTGTTGTG CCTGAAGGTG CTTTCAATGA TCTTTCTGCA 
TTATCACATC TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA 
TCCGACTGGG TGAAGTCGGA ATATAAGGAG CCTGGAATTG CTCGTTGTGC TGGTCCTGGA 
GAAATGGCAG ATAAACTTTT ACTCACAACT CCCTCCAAAA AATTTACCTG TCAAGGTCCT 
GTGGATGTCA ATATTCTAGC TAAGTGTAAC CCCTGCCTAT CAAATCCGTG TAAAAATGAT 
GGCACATGTA ATAGTGATCC AGTTGACTTT TACCGATGCA CCTGTCCATA TGGTTTCAAG 
GGGCAGGACT GTGATGTCCC AATTCATGCC TGCATCAGTA ACCCATGTAA ACATGGAGGA 
ACTTGCCACT TAAAGGAAGG AGAAGAAGAT GGATTCTGGT GTATTTGTGC TGATGGATTT 
GAAGGAGAAA ATTGTGAAGT CAACGTTGAT GATTGTGAAG ATAATGACTG TGAAAATAAT 
TCTACATGTG TCGATGGCAT TAATAACTAC ACATGCCTTT GCCCACCTGA GTATACAGGT 
GAGTTGTGTG AGGAGAAGCT GGACTTCTGT GCCCAGGACC TGAACCCCTG CCAGCACGAT 
TCAAAGTGCA TCCTAACTCC AAAGGGATTC AAATGTGACT GCACACCAGG GTACGTAGGT 
GAACACTGCG ACATCGATTT TGACGACTGC CAAGACAACA AGTGTAAAAA CGGAGCCCAC 
TGCACAGATG CAGTGAACGG CTATACGTGC ATATGCCCCG AAGGTTACAG TGGCTTGTTC 
TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGTACCAGCC CCTGTGATAA TTTTGATTGT 
CAGAATGGAG CTCAGTGTAT CGTCAGAATA AATGAGCCAA TATGTCAGTG TTTGCCTGGC 
TATCAGGGAG AAAAGTGTGA AAAATTGGTT AGTGTGAATT TTATAAACAA AGAGTCTTAT 
CTTCAGATTC CTTCAGCCAA GGTTCGGCCT CAGACGAACA TAACACTTCA GATTGCCACA 
GATGAAGACA GCGGAATCCT CCTGTATAAG GGTGACAAAG ACCATATCGC GGTAGAACTC 
TATCGGGGGC GTGTTCGTGC CAGCTATGAC ACCGGCTCTC ATCCAGCTTC TGCCATTTAC 
AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTGG AACTACTTGC CTTGGATCAG 
AGTCTCTCTT TGTCCGTGGA TGGTGGGAAC CCCAAAATCA TCACTAACTT GTCAAAGCAG 
TCCACTCTGA ATTTTGACTC TCCACTCTAT GTAGGAGGCA TGCCAGGGAA GAGTAACGTG 
GCATCTCTGC GCCAGGCCCC TGGGCAGAAC GGAACCAGCT TCCACGGCTG CATCCGGAAC 
CTTTACATCA ACAGTGAGCT GCAGGACTTC CAGAAGGTGC CGATGCAAAC AGGCATTTTG 
CCTGGCTGTG AGCCATGCCA CAAGAAGGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC 
CAGGCAGGCT TCACCTGCGA GTGCCAGGAA GGATGGATGG GGCCCCTCTG TGACCAACGG 
ACCAATGACC CTTGCCTTGG AAATAAATGC GTACATGGCA CCTGCTTGCC CATCAATGCG 
TTCTCCTACA GCTGTAAGTG CTTGGAGGGC CATGGAGGTG TCCTCTGTGA TGAAGAGGAG 
GATCTGTTTA ACCCATGCCA GGCGATCAAG TGCAAGCATG GGAAGTGCAG GCTTTCAGGT 
CTGGGGCAGC CCTACTGTGA ATGCAGCAGT GGATACACGG GGGACAGCTG TGATCGAGAA 
ATCTCTTGTC GAGGGGAAAG GATAAGAGAT TATTACCAAA AGCAGCAGGG CTATGCTGCT 
TGCCAAACAA CCAAGAAGGT GTCCCGATTA GAGTGCAGAG GTGGGTGTGC AGGAGGGCAG 
TGCTGTGGAC CGCTGAGGAG CAAGCGGCGG AAATACTCTT TCGAATGCAC TGACGGCTCC 
TCCTTTGTGG ACGAGGTTGA GAAAGTGGTG AAGTGCGGCT GTACGAGGTG TGTGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SLIT2
Entrez Gene ID	9353
Full Name	slit guidance ligand 2
Synonyms	SLIL3,Slit-2
General protein information	Preferred Names slit guidance ligand 2 Names slit homolog 2 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p15.31
Summary	This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].
Disorder MIM:	603746

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SLIT2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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Target ORF information:

ORF Insert Sequence:

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