UBE2L6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2L6
Entrez Gene ID	9246
Full Name	ubiquitin conjugating enzyme E2 L6
Synonyms	RIG-B,UBCH8
General protein information	Preferred Names ubiquitin conjugating enzyme E2 L6 Names ubiquitin/ISG15-conjugating enzyme E2 L6 E2 ubiquitin-conjugating enzyme L6 retinoic acid induced gene B protein ubiquitin carrier protein L6 ubiquitin conjugating enzyme E2L 6 ubiquitin-protein ligase L6
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.1
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011].
Disorder MIM:	603890

mRNA and Protein(s)

mRNA	Protein	Name
NM_004223.5	NP_004214.1	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
NM_004223.4	NP_004214.1	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
NM_198183.2	NP_937826.1	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ube2l6	NP_001019926.1
Homo sapiens (human)	UBE2L6	NP_004214.1
Canis lupus familiaris (dog)	UBE2L6	XP_533174.1
Pan troglodytes (chimpanzee)	UBE2L6	XP_003318028.1
Bos taurus (cattle)	UBE2L6	NP_001092387.1
Mus musculus (house mouse)	Ube2l6	NP_064333.2

Gene Ontology
Bibliography

Related articles in PubMed

UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells.
Zhang Q, Qiao L, Wang X, Ding C, Chen JJ
Cell cycle (Georgetown, Tex.)1-22(2017 Nov)

UBE2L6/UBCH8 and ISG15 attenuate autophagy in esophageal cancer cells.
Falvey CM, O'Donovan TR, El-Mashed S, Nyhan MJ, O'Reilly S, McKenna SL
Oncotarget8(14)23479-23491(2017 Apr)

Epigenetic downregulation of the ISG15-conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma.
Zhou X, Wei J, Chen F, Xiao X, Huang T, He Q, Wang S, Du C, Mo Y, Lin L, Xie Y, Wei L, Lan Y, Murata M, Huang G, Ernberg I, Matskova L, Zhang Z
Oncotarget6(38)41077-91(2015 Dec)

E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis.
Pruneda JN, Smith FD, Daurie A, Swaney DL, Vill?n J, Scott JD, Stadnyk AW, Le Trong I, Stenkamp RE, Klevit RE, Rohde JR, Brzovic PS
The EMBO journal33(5)437-49(2014 Mar)

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A
Molecular and cellular biology31(15)3136-45(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Depletion of either ISG15 or UBE2L6/UBCH8 resulted in enhanced endogenous autophagic flux.
Title: UBE2L6/UBCH8 and ISG15 attenuate autophagy in esophageal cancer cells.

Data indicate that reduced expression of the ubiquitin-conjugating enzyme UbcH8 protein correlated with poor outcome in nasopharyngeal carcinoma (NPC) patients.
Title: Epigenetic downregulation of the ISG15-conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma.

study reports that UBCH8 and UBE2G1 and UBE2G2 cooperate with CRL4Cdt2 in promoting the polyubiquitylation and subsequent degradation of p21 and Cdt1, respectively
Title: Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.

gain and loss-of-function approaches show that UBCH8 and the ubiquitin-ligase SIAH1 physically interact with and target FLT3-ITD for proteasomal degradation
Title: Ubiquitin conjugase UBCH8 targets active FMS-like tyrosine kinase 3 for proteasomal degradation.

The structure of UbcH8 does not undergo a significant conformational change upon forming a complex with ubiquitin.
Title: The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site.

The following UBE2L6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2L6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02498	NM_198183.2 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04576	NM_004223.5 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04576	NM_004223.4	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2L6 ( NM_198183.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937826.1 Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02498
Clone ID Related Accession (Same CDS sequence)	NM_198183.2
Accession Version	NM_198183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA097307.1, AK093462.1 and H17887.1. On May 4, 2011 this sequence version replaced NM_198183.1. Transcript Variant: This variant (2) contains an alternate 5' exon and uses a downstream start codon, as compared to variant 1. The encoded isoform (2) is thus shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.450190.1, SRR1163658.251015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAAT TCACAACCAA GATCTACCAC CCCAACGTGG ACGAGAACGG ACAGATTTGC 
CTGCCCATCA TCAGCAGTGA GAACTGGAAG CCTTGCACCA AGACTTGCCA AGTCCTGGAG 
GCCCTCAATG TGCTGGTGAA TAGACCGAAT ATCAGGGAGC CCCTGCGGAT GGACCTCGCT 
GACCTGCTGA CACAGAATCC GGAGCTGTTC AGAAAGAATG CCGAAGAGTT CACCCTCCGA 
TTCGGAGTGG ACCGGCCCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937826.1
CDS	654..917
Translation	MIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS

Target ORF information:

RefSeq Version	NM_198183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198183.2

ORF Insert Sequence:

ATGATCAAAT TCACAACCAA GATCTACCAC CCCAACGTGG ACGAGAACGG ACAGATTTGC 
CTGCCCATCA TCAGCAGTGA GAACTGGAAG CCTTGCACCA AGACTTGCCA AGTCCTGGAG 
GCCCTCAATG TGCTGGTGAA TAGACCGAAT ATCAGGGAGC CCCTGCGGAT GGACCTCGCT 
GACCTGCTGA CACAGAATCC GGAGCTGTTC AGAAAGAATG CCGAAGAGTT CACCCTCCGA 
TTCGGAGTGG ACCGGCCCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2L6 ( NM_004223.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004214.1 Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04576
Clone ID Related Accession (Same CDS sequence)	NM_004223.5 , NM_004223.4
Accession Version	NM_004223.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032491.1 and H17887.1. On Nov 22, 2018 this sequence version replaced NM_004223.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032491.1, SRR3476690.293196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000287156.9/ ENSP00000287156.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGGCGA GCATGCGAGT GGTGAAGGAG CTGGAGGATC TTCAGAAGAA GCCTCCCCCA 
TACCTGCGGA ACCTGTCCAG CGATGATGCC AATGTCCTGG TGTGGCACGC TCTCCTCCTA 
CCCGACCAAC CTCCCTACCA CCTGAAAGCC TTCAACCTGC GCATCAGCTT CCCGCCGGAG 
TATCCGTTCA AGCCTCCCAT GATCAAATTC ACAACCAAGA TCTACCACCC CAACGTGGAC 
GAGAACGGAC AGATTTGCCT GCCCATCATC AGCAGTGAGA ACTGGAAGCC TTGCACCAAG 
ACTTGCCAAG TCCTGGAGGC CCTCAATGTG CTGGTGAATA GACCGAATAT CAGGGAGCCC 
CTGCGGATGG ACCTCGCTGA CCTGCTGACA CAGAATCCGG AGCTGTTCAG AAAGAATGCC 
GAAGAGTTCA CCCTCCGATT CGGAGTGGAC CGGCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004214.1
CDS	62..523
Translation	MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS

Target ORF information:

RefSeq Version	NM_004223.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004223.5

ORF Insert Sequence:

ATGATGGCGA GCATGCGAGT GGTGAAGGAG CTGGAGGATC TTCAGAAGAA GCCTCCCCCA 
TACCTGCGGA ACCTGTCCAG CGATGATGCC AATGTCCTGG TGTGGCACGC TCTCCTCCTA 
CCCGACCAAC CTCCCTACCA CCTGAAAGCC TTCAACCTGC GCATCAGCTT CCCGCCGGAG 
TATCCGTTCA AGCCTCCCAT GATCAAATTC ACAACCAAGA TCTACCACCC CAACGTGGAC 
GAGAACGGAC AGATTTGCCT GCCCATCATC AGCAGTGAGA ACTGGAAGCC TTGCACCAAG 
ACTTGCCAAG TCCTGGAGGC CCTCAATGTG CTGGTGAATA GACCGAATAT CAGGGAGCCC 
CTGCGGATGG ACCTCGCTGA CCTGCTGACA CAGAATCCGG AGCTGTTCAG AAAGAATGCC 
GAAGAGTTCA CCCTCCGATT CGGAGTGGAC CGGCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2L6 ( NM_004223.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004214.1 Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04576
Clone ID Related Accession (Same CDS sequence)	NM_004223.5 , NM_004223.4
Accession Version	NM_004223.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	ubiquitin/ISG15-conjugating enzyme E2 L6 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC032491.1 and H17887.1. On May 4, 2011 this sequence version replaced NM_004223.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032491.1, SRR1163657.428854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCGA GCATGCGAGT GGTGAAGGAG CTGGAGGATC TTCAGAAGAA GCCTCCCCCA 
TACCTGCGGA ACCTGTCCAG CGATGATGCC AATGTCCTGG TGTGGCACGC TCTCCTCCTA 
CCCGACCAAC CTCCCTACCA CCTGAAAGCC TTCAACCTGC GCATCAGCTT CCCGCCGGAG 
TATCCGTTCA AGCCTCCCAT GATCAAATTC ACAACCAAGA TCTACCACCC CAACGTGGAC 
GAGAACGGAC AGATTTGCCT GCCCATCATC AGCAGTGAGA ACTGGAAGCC TTGCACCAAG 
ACTTGCCAAG TCCTGGAGGC CCTCAATGTG CTGGTGAATA GACCGAATAT CAGGGAGCCC 
CTGCGGATGG ACCTCGCTGA CCTGCTGACA CAGAATCCGG AGCTGTTCAG AAAGAATGCC 
GAAGAGTTCA CCCTCCGATT CGGAGTGGAC CGGCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004214.1
CDS	97..558
Translation	MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS

Target ORF information:

RefSeq Version	NM_004223.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 L6 (UBE2L6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004223.4

ORF Insert Sequence:

ATGATGGCGA GCATGCGAGT GGTGAAGGAG CTGGAGGATC TTCAGAAGAA GCCTCCCCCA 
TACCTGCGGA ACCTGTCCAG CGATGATGCC AATGTCCTGG TGTGGCACGC TCTCCTCCTA 
CCCGACCAAC CTCCCTACCA CCTGAAAGCC TTCAACCTGC GCATCAGCTT CCCGCCGGAG 
TATCCGTTCA AGCCTCCCAT GATCAAATTC ACAACCAAGA TCTACCACCC CAACGTGGAC 
GAGAACGGAC AGATTTGCCT GCCCATCATC AGCAGTGAGA ACTGGAAGCC TTGCACCAAG 
ACTTGCCAAG TCCTGGAGGC CCTCAATGTG CTGGTGAATA GACCGAATAT CAGGGAGCCC 
CTGCGGATGG ACCTCGCTGA CCTGCTGACA CAGAATCCGG AGCTGTTCAG AAAGAATGCC 
GAAGAGTTCA CCCTCCGATT CGGAGTGGAC CGGCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells.
Cell cycle (Georgetown, Tex.)1-22(2017 Nov)
Zhang Q,Qiao L,Wang X,Ding C,Chen JJ

UBE2L6/UBCH8 and ISG15 attenuate autophagy in esophageal cancer cells.
Oncotarget8(14)23479-23491(2017 Apr)
Falvey CM,O'Donovan TR,El-Mashed S,Nyhan MJ,O'Reilly S,McKenna SL

Epigenetic downregulation of the ISG15-conjugating enzyme UbcH8 impairs lipolysis and correlates with poor prognosis in nasopharyngeal carcinoma.
Oncotarget6(38)41077-91(2015 Dec)
Zhou X,Wei J,Chen F,Xiao X,Huang T,He Q,Wang S,Du C,Mo Y,Lin L,Xie Y,Wei L,Lan Y,Murata M,Huang G,Ernberg I,Matskova L,Zhang Z

E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis.
The EMBO journal33(5)437-49(2014 Mar)
Pruneda JN,Smith FD,Daurie A,Swaney DL,Vill?n J,Scott JD,Stadnyk AW,Le Trong I,Stenkamp RE,Klevit RE,Rohde JR,Brzovic PS

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Molecular and cellular biology31(15)3136-45(2011 Aug)
Shibata E,Abbas T,Huang X,Wohlschlegel JA,Dutta A

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UBE2L6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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