SEMA5A cDNA ORF clone, Homo sapiens(human)

The following SEMA5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18471 NM_003966.3
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514157.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514156.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_006714506.3
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514155.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_006714507.3
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514158.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu96183 XM_017010016.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu62398 XM_011514159.2
Latest version!
Homo sapiens semaphorin 5A (SEMA5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu18471 XM_006714507.2 Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 NM_003966.2 Homo sapiens semaphorin 5A (SEMA5A), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514155.1 Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu96183 XM_017010016.1 Homo sapiens semaphorin 5A (SEMA5A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu18471 XM_011514156.1 Homo sapiens semaphorin 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514157.1 Homo sapiens semaphorin 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_006714506.2 Homo sapiens semaphorin 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu18471 XM_011514158.1 Homo sapiens semaphorin 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version NM_003966.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-26
    Organism Homo sapiens(human)
    Product semaphorin-5A precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC034214.5, AC027335.6, AC091906.3, AC022446.6, AC027336.4 and AC021088.5. On Nov 23, 2018 this sequence version replaced NM_003966.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307587.1, U52840.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000382496.10/ ENSP00000371936.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003957.2
    CDS667..3891
    Translation

    Target ORF information:

    RefSeq Version NM_003966.3
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003966.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version NM_003966.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product semaphorin-5A precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA336109.1, BC115696.1, U52840.1, CX788776.1, AC027336.4, AC021088.5 and BM669539.1. This sequence is a reference standard in the RefSeqGene project. On May 22, 2007 this sequence version replaced NM_003966.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U52840.1, BC115696.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003957.2
    CDS713..3937
    Translation

    Target ORF information:

    RefSeq Version NM_003966.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_006714506.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714569.1
    CDS858..4082
    Translation

    Target ORF information:

    RefSeq Version XM_006714506.3
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714506.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_006714507.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product semaphorin-5A isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714570.1
    Translation

    Target ORF information:

    RefSeq Version XM_006714507.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714507.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514156.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512458.1
    CDS885..4109
    Translation

    Target ORF information:

    RefSeq Version XM_011514156.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514155.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512457.1
    CDS336..3560
    Translation

    Target ORF information:

    RefSeq Version XM_011514155.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514155.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_006714507.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714570.1
    CDS375..3599
    Translation

    Target ORF information:

    RefSeq Version XM_006714507.3
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714507.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514158.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512460.1
    CDS572..3796
    Translation

    Target ORF information:

    RefSeq Version XM_011514158.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514155.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product semaphorin-5A isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512457.1
    Translation

    Target ORF information:

    RefSeq Version XM_011514155.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514157.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512459.1
    CDS786..4010
    Translation

    Target ORF information:

    RefSeq Version XM_011514157.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514157.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514156.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512458.1
    CDS931..4155
    Translation

    Target ORF information:

    RefSeq Version XM_011514156.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514157.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512459.1
    CDS740..3964
    Translation

    Target ORF information:

    RefSeq Version XM_011514157.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_006714506.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006714506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714569.1
    CDS812..4036
    Translation

    Target ORF information:

    RefSeq Version XM_006714506.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18471
    Clone ID Related Accession (Same CDS sequence) XM_006714506.3 , XM_006714507.2 , XM_011514156.1 , NM_003966.3 , XM_011514155.2 , XM_006714507.3 , XM_011514158.2 , NM_003966.2 , XM_011514155.1 , XM_011514157.2 , XM_011514156.2 , XM_011514157.1 , XM_006714506.2 , XM_011514158.1
    Accession Version XM_011514158.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512460.1
    CDS570..3794
    Translation

    Target ORF information:

    RefSeq Version XM_011514158.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAA CCTGTGTTAT AGCATGGCTG TTCTCAAGCC TGGGGCTGTG GAGACTCGCC 
    CACCCAGAGG CCCAGGGTAC GACTCAGTGC CAGAGAACCG AGCATCCAGT CATCTCCTAT
    AAAGAAATTG GCCCCTGGTT ACGGGAGTTC AGAGCGAAGA ATGCTGTGGA TTTCTCGCAG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTTGTAGGAG CAAGAAACTA CCTCTTCAGG
    TTACAGCTTG AGGATCTGTC TCTTATCCAG GCTGTGGAAT GGGAGTGTGA TGAAGCTACC
    AAAAAGGCCT GTTACAGCAA AGGCAAATCA AAGGAGGAAT GTCAGAACTA CATCCGGGTG
    CTTCTGGTGG GTGGCGACCG GTTATTCACC TGTGGGACCA ATGCATTCAC GCCTGTCTGC
    ACCAACCGCT CGTTGAGCAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCCCGC
    TGTCCCTACA GTCCCCAGCA CAATTCCACA GCGCTCCTCA CAGCTGGTGG GGAGCTCTAT
    GCTGCTACAG CCATGGATTT TCCAGGACGT GATCCTGCCA TTTACCGAAG CCTAGGCATT
    TTACCTCCTC TCCGCACGGC GCAGTACAAC TCCAAATGGC TCAATGAGCC AAACTTTGTG
    TCATCTTATG ACATCGGAAA TTTTACCTAC TTCTTTTTCC GAGAAAATGC AGTAGAGCAT
    GACTGTGGGA AAACAGTGTT CTCCAGAGCT GCCCGGGTGT GCAAGAACGA TATTGGTGGG
    CGCTTCCTGC TGGAAGACAC CTGGACCACA TTCATGAAGG CTCGCCTGAA CTGCTCCCGT
    CCTGGGGAAG TCCCCTTTTA CTACAACGAA TTGCAGAGTA CTTTCTTCCT GCCTGAGCTG
    GATTTGATCT ATGGCATCTT TACCACCAAT GTGAACAGCA TTGCGGCCTC AGCTGTGTGC
    GTCTTCAACC TGAGCGCCAT CGCGCAGGCC TTCTCTGGGC CCTTCAAGTA CCAAGAAAAC
    TCGCGCTCGG CCTGGCTACC GTATCCCAAC CCAAACCCCC ACTTCCAGTG TGGCACCGTG
    GACCAGGGCC TGTACGTGAA CCTGACCGAG AGAAATCTGC AGGATGCTCA GAAGTTCATT
    CTGATGCATG AGGTGGTACA GCCAGTGACC ACAGTGCCCT CCTTCATGGA GGACAATAGC
    CGCTTTTCCC ACGTGGCAGT CGACGTGGTG CAGGGCAGAG AAGCGCTCGT CCACATCATC
    TATTTGGCCA CAGATTACGG AACCATTAAG AAAGTGCGGG TACCCCTGAA TCAGACCTCA
    AGCAGCTGTT TGCTGGAAGA GATTGAGCTC TTCCCTGAGA GGCGGAGGGA GCCCATCAGG
    AGCCTGCAGA TCCTGCACAG CCAGAGTGTC CTGTTCGTGG GCCTGCGGGA GCACGTGGTC
    AAGATCCCCC TGAAGAGGTG CCAGTTCTAC CGCACACGCA GCACCTGCAT TGGGGCCCAG
    GACCCTTACT GTGGCTGGGA TGTGGTAATG AAGAAATGCA CAAGCCTGGA GGAGAGCCTG
    AGCATGACGC AGTGGGAACA GAGCATCTCT GCGTGTCCGA CCAGGAATCT CACCGTGGAT
    GGGCACTTTG GTGTGTGGTC TCCGTGGACG CCTTGCACGC ACACAGATGG CAGCGCCGTG
    GGATCCTGCC TCTGTCGAAC CCGCTCCTGC GACAGCCCGG CCCCGCAGTG TGGTGGCTGG
    CAGTGCGAGG GCCCTGGCAT GGAGATCGCC AACTGTTCCA GGAACGGAGG CTGGACTCCC
    TGGACCTCGT GGTCTCCCTG CAGCACTACC TGTGGGATCG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCCACTCC CAGGCACGGG GGCCGGGTGT GCGTGGGACA GAACCGCGAG
    GAAAGATACT GCAATGAACA TTTGCTATGT CCCCCACACA TGTTCTGGAC AGGCTGGGGT
    CCTTGGGAAC GGTGCACAGC CCAATGCGGG GGTGGCATTC AAGCTCGCCG CAGGATCTGT
    GAGAATGGGC CTGACTGTGC AGGCTGCAAT GTGGAGTACC AGTCTTGCAA CACCAACCCG
    TGTCCTGAGC TGAAGAAGAC CACGCCCTGG ACACCCTGGA CACCTGTCAA CATCTCTGAC
    AACGGCGGCC ACTATGAGCA ACGATTCCGA TACACATGCA AAGCCCGCCT GGCTGATCCG
    AATTTGCTGG AAGTGGGAAG ACAGAGAATC GAAATGCGGT ACTGTTCTAG CGACGGCACC
    AGTGGCTGCT CCACAGATGG GCTTTCTGGG GATTTCCTGC GTGCTGGGAG ATACTCTGCC
    CACACGGTCA ACGGGGCTTG GTCAGCCTGG ACGTCGTGGT CACAGTGCAG CCGTGACTGC
    AGCAGGGGCA TTCGGAACCG GAAGCGTGTT TGCAACAACC CCGAACCCAA GTATGGGGGA
    ATGCCTTGCC TTGGCCCATC TCTGGAATAC CAGGAATGCA ACATTTTGCC CTGCCCAGTG
    GATGGCGTGT GGTCTTGCTG GTCCCCCTGG ACAAAATGTT CAGCAACATG CGGCGGTGGA
    CACTATATGA GGACCCGCTC TTGCTCCAAT CCAGCCCCGG CCTATGGAGG GGACATCTGC
    CTGGGGCTGC ACACAGAAGA GGCACTCTGC AACACGCAGC CCTGCCCAGA GAGCTGGTCG
    GAGTGGTCGG ACTGGTCTGA GTGTGAAGCC TCTGGCGTCC AAGTCCGCGC CCGCCAGTGC
    ATCCTCCTGT TCCCCATGGG CAGCCAGTGC TCCGGGAACA CCACGGAGAG CCGGCCGTGT
    GTGTTTGACT CTAATTTCAT CCCAGAAGTA TCTGTGGCAA GATCCAGTAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATCGCCG TGGGGCTGAG CAGCTCCATC
    CTCGGCTGCC TCCTCACCCT GCTCGTCTAT ACTTACTGCC AGCGGTACCA GCAGCAATCC
    CACGATGCGA CTGTCATCCA CCCCGTCTCA CCTGCCCCCC TTAATACCAG CATAACCAAC
    CACATCAACA AACTGGACAA GTACGACTCG GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAAAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAT
    TCTAATGCCT ACTTTACAGA TCTCAATAAT TATGATGAGT ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96183
    Clone ID Related Accession (Same CDS sequence) XM_017010016.2 , XM_017010016.1
    Accession Version XM_017010016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCCGCT GTCCCTACAG TCCCCAGCAC AATTCCACAG CGCTCCTCAC AGCTGGTGGG 
    GAGCTCTATG CTGCTACAGC CATGGATTTT CCAGGACGTG ATCCTGCCAT TTACCGAAGC
    CTAGGCATTT TACCTCCTCT CCGCACGGCG CAGTACAACT CCAAATGGCT CAATGAGCCA
    AACTTTGTGT CATCTTATGA CATCGGAAAT TTTACCTACT TCTTTTTCCG AGAAAATGCA
    GTAGAGCATG ACTGTGGGAA AACAGTGTTC TCCAGAGCTG CCCGGGTGTG CAAGAACGAT
    ATTGGTGGGC GCTTCCTGCT GGAAGACACC TGGACCACAT TCATGAAGGC TCGCCTGAAC
    TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC TACAACGAAT TGCAGAGTAC TTTCTTCCTG
    CCTGAGCTGG ATTTGATCTA TGGCATCTTT ACCACCAATG TGAACAGCAT TGCGGCCTCA
    GCTGTGTGCG TCTTCAACCT GAGCGCCATC GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC
    CAAGAAAACT CGCGCTCGGC CTGGCTACCG TATCCCAACC CAAACCCCCA CTTCCAGTGT
    GGCACCGTGG ACCAGGGCCT GTACGTGAAC CTGACCGAGA GAAATCTGCA GGATGCTCAG
    AAGTTCATTC TGATGCATGA GGTGGTACAG CCAGTGACCA CAGTGCCCTC CTTCATGGAG
    GACAATAGCC GCTTTTCCCA CGTGGCAGTC GACGTGGTGC AGGGCAGAGA AGCGCTCGTC
    CACATCATCT ATTTGGCCAC AGATTACGGA ACCATTAAGA AAGTGCGGGT ACCCCTGAAT
    CAGACCTCAA GCAGCTGTTT GCTGGAAGAG ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG
    CCCATCAGGA GCCTGCAGAT CCTGCACAGC CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG
    CACGTGGTCA AGATCCCCCT GAAGAGGTGC CAGTTCTACC GCACACGCAG CACCTGCATT
    GGGGCCCAGG ACCCTTACTG TGGCTGGGAT GTGGTAATGA AGAAATGCAC AAGCCTGGAG
    GAGAGCCTGA GCATGACGCA GTGGGAACAG AGCATCTCTG CGTGTCCGAC CAGGAATCTC
    ACCGTGGATG GGCACTTTGG TGTGTGGTCT CCGTGGACGC CTTGCACGCA CACAGATGGC
    AGCGCCGTGG GATCCTGCCT CTGTCGAACC CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT
    GGTGGCTGGC AGTGCGAGGG CCCTGGCATG GAGATCGCCA ACTGTTCCAG GAACGGAGGC
    TGGACTCCCT GGACCTCGTG GTCTCCCTGC AGCACTACCT GTGGGATCGG CTTCCAGGTG
    CGGCAGCGCT CCTGCAGCAA CCCCACTCCC AGGCACGGGG GCCGGGTGTG CGTGGGACAG
    AACCGCGAGG AAAGATACTG CAATGAACAT TTGCTATGTC CCCCACACAT GTTCTGGACA
    GGCTGGGGTC CTTGGGAACG GTGCACAGCC CAATGCGGGG GTGGCATTCA AGCTCGCCGC
    AGGATCTGTG AGAATGGGCC TGACTGTGCA GGCTGCAATG TGGAGTACCA GTCTTGCAAC
    ACCAACCCGT GTCCTGAGCT GAAGAAGACC ACGCCCTGGA CACCCTGGAC ACCTGTCAAC
    ATCTCTGACA ACGGCGGCCA CTATGAGCAA CGATTCCGAT ACACATGCAA AGCCCGCCTG
    GCTGATCCGA ATTTGCTGGA AGTGGGAAGA CAGAGAATCG AAATGCGGTA CTGTTCTAGC
    GACGGCACCA GTGGCTGCTC CACAGATGGG CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA
    TACTCTGCCC ACACGGTCAA CGGGGCTTGG TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC
    CGTGACTGCA GCAGGGGCAT TCGGAACCGG AAGCGTGTTT GCAACAACCC CGAACCCAAG
    TATGGGGGAA TGCCTTGCCT TGGCCCATCT CTGGAATACC AGGAATGCAA CATTTTGCCC
    TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG TCCCCCTGGA CAAAATGTTC AGCAACATGC
    GGCGGTGGAC ACTATATGAG GACCCGCTCT TGCTCCAATC CAGCCCCGGC CTATGGAGGG
    GACATCTGCC TGGGGCTGCA CACAGAAGAG GCACTCTGCA ACACGCAGCC CTGCCCAGAG
    AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC
    CGCCAGTGCA TCCTCCTGTT CCCCATGGGC AGCCAGTGCT CCGGGAACAC CACGGAGAGC
    CGGCCGTGTG TGTTTGACTC TAATTTCATC CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC
    GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC ATGTTCCACA TGATCGCCGT GGGGCTGAGC
    AGCTCCATCC TCGGCTGCCT CCTCACCCTG CTCGTCTATA CTTACTGCCA GCGGTACCAG
    CAGCAATCCC ACGATGCGAC TGTCATCCAC CCCGTCTCAC CTGCCCCCCT TAATACCAGC
    ATAACCAACC ACATCAACAA ACTGGACAAG TACGACTCGG TGGAGGCCAT CAAGGCATTT
    AACAAAAACA ACTTGATCCT AGAGGAAAGA AACAAATACT TCAACCCACA TCTCACTGGG
    AAGACCTATT CTAATGCCTA CTTTACAGAT CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865505.1
    CDS1084..3837
    Translation

    Target ORF information:

    RefSeq Version XM_017010016.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCCGCT GTCCCTACAG TCCCCAGCAC AATTCCACAG CGCTCCTCAC AGCTGGTGGG 
    GAGCTCTATG CTGCTACAGC CATGGATTTT CCAGGACGTG ATCCTGCCAT TTACCGAAGC
    CTAGGCATTT TACCTCCTCT CCGCACGGCG CAGTACAACT CCAAATGGCT CAATGAGCCA
    AACTTTGTGT CATCTTATGA CATCGGAAAT TTTACCTACT TCTTTTTCCG AGAAAATGCA
    GTAGAGCATG ACTGTGGGAA AACAGTGTTC TCCAGAGCTG CCCGGGTGTG CAAGAACGAT
    ATTGGTGGGC GCTTCCTGCT GGAAGACACC TGGACCACAT TCATGAAGGC TCGCCTGAAC
    TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC TACAACGAAT TGCAGAGTAC TTTCTTCCTG
    CCTGAGCTGG ATTTGATCTA TGGCATCTTT ACCACCAATG TGAACAGCAT TGCGGCCTCA
    GCTGTGTGCG TCTTCAACCT GAGCGCCATC GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC
    CAAGAAAACT CGCGCTCGGC CTGGCTACCG TATCCCAACC CAAACCCCCA CTTCCAGTGT
    GGCACCGTGG ACCAGGGCCT GTACGTGAAC CTGACCGAGA GAAATCTGCA GGATGCTCAG
    AAGTTCATTC TGATGCATGA GGTGGTACAG CCAGTGACCA CAGTGCCCTC CTTCATGGAG
    GACAATAGCC GCTTTTCCCA CGTGGCAGTC GACGTGGTGC AGGGCAGAGA AGCGCTCGTC
    CACATCATCT ATTTGGCCAC AGATTACGGA ACCATTAAGA AAGTGCGGGT ACCCCTGAAT
    CAGACCTCAA GCAGCTGTTT GCTGGAAGAG ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG
    CCCATCAGGA GCCTGCAGAT CCTGCACAGC CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG
    CACGTGGTCA AGATCCCCCT GAAGAGGTGC CAGTTCTACC GCACACGCAG CACCTGCATT
    GGGGCCCAGG ACCCTTACTG TGGCTGGGAT GTGGTAATGA AGAAATGCAC AAGCCTGGAG
    GAGAGCCTGA GCATGACGCA GTGGGAACAG AGCATCTCTG CGTGTCCGAC CAGGAATCTC
    ACCGTGGATG GGCACTTTGG TGTGTGGTCT CCGTGGACGC CTTGCACGCA CACAGATGGC
    AGCGCCGTGG GATCCTGCCT CTGTCGAACC CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT
    GGTGGCTGGC AGTGCGAGGG CCCTGGCATG GAGATCGCCA ACTGTTCCAG GAACGGAGGC
    TGGACTCCCT GGACCTCGTG GTCTCCCTGC AGCACTACCT GTGGGATCGG CTTCCAGGTG
    CGGCAGCGCT CCTGCAGCAA CCCCACTCCC AGGCACGGGG GCCGGGTGTG CGTGGGACAG
    AACCGCGAGG AAAGATACTG CAATGAACAT TTGCTATGTC CCCCACACAT GTTCTGGACA
    GGCTGGGGTC CTTGGGAACG GTGCACAGCC CAATGCGGGG GTGGCATTCA AGCTCGCCGC
    AGGATCTGTG AGAATGGGCC TGACTGTGCA GGCTGCAATG TGGAGTACCA GTCTTGCAAC
    ACCAACCCGT GTCCTGAGCT GAAGAAGACC ACGCCCTGGA CACCCTGGAC ACCTGTCAAC
    ATCTCTGACA ACGGCGGCCA CTATGAGCAA CGATTCCGAT ACACATGCAA AGCCCGCCTG
    GCTGATCCGA ATTTGCTGGA AGTGGGAAGA CAGAGAATCG AAATGCGGTA CTGTTCTAGC
    GACGGCACCA GTGGCTGCTC CACAGATGGG CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA
    TACTCTGCCC ACACGGTCAA CGGGGCTTGG TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC
    CGTGACTGCA GCAGGGGCAT TCGGAACCGG AAGCGTGTTT GCAACAACCC CGAACCCAAG
    TATGGGGGAA TGCCTTGCCT TGGCCCATCT CTGGAATACC AGGAATGCAA CATTTTGCCC
    TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG TCCCCCTGGA CAAAATGTTC AGCAACATGC
    GGCGGTGGAC ACTATATGAG GACCCGCTCT TGCTCCAATC CAGCCCCGGC CTATGGAGGG
    GACATCTGCC TGGGGCTGCA CACAGAAGAG GCACTCTGCA ACACGCAGCC CTGCCCAGAG
    AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC
    CGCCAGTGCA TCCTCCTGTT CCCCATGGGC AGCCAGTGCT CCGGGAACAC CACGGAGAGC
    CGGCCGTGTG TGTTTGACTC TAATTTCATC CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC
    GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC ATGTTCCACA TGATCGCCGT GGGGCTGAGC
    AGCTCCATCC TCGGCTGCCT CCTCACCCTG CTCGTCTATA CTTACTGCCA GCGGTACCAG
    CAGCAATCCC ACGATGCGAC TGTCATCCAC CCCGTCTCAC CTGCCCCCCT TAATACCAGC
    ATAACCAACC ACATCAACAA ACTGGACAAG TACGACTCGG TGGAGGCCAT CAAGGCATTT
    AACAAAAACA ACTTGATCCT AGAGGAAAGA AACAAATACT TCAACCCACA TCTCACTGGG
    AAGACCTATT CTAATGCCTA CTTTACAGAT CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96183
    Clone ID Related Accession (Same CDS sequence) XM_017010016.2 , XM_017010016.1
    Accession Version XM_017010016.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCCGCT GTCCCTACAG TCCCCAGCAC AATTCCACAG CGCTCCTCAC AGCTGGTGGG 
    GAGCTCTATG CTGCTACAGC CATGGATTTT CCAGGACGTG ATCCTGCCAT TTACCGAAGC
    CTAGGCATTT TACCTCCTCT CCGCACGGCG CAGTACAACT CCAAATGGCT CAATGAGCCA
    AACTTTGTGT CATCTTATGA CATCGGAAAT TTTACCTACT TCTTTTTCCG AGAAAATGCA
    GTAGAGCATG ACTGTGGGAA AACAGTGTTC TCCAGAGCTG CCCGGGTGTG CAAGAACGAT
    ATTGGTGGGC GCTTCCTGCT GGAAGACACC TGGACCACAT TCATGAAGGC TCGCCTGAAC
    TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC TACAACGAAT TGCAGAGTAC TTTCTTCCTG
    CCTGAGCTGG ATTTGATCTA TGGCATCTTT ACCACCAATG TGAACAGCAT TGCGGCCTCA
    GCTGTGTGCG TCTTCAACCT GAGCGCCATC GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC
    CAAGAAAACT CGCGCTCGGC CTGGCTACCG TATCCCAACC CAAACCCCCA CTTCCAGTGT
    GGCACCGTGG ACCAGGGCCT GTACGTGAAC CTGACCGAGA GAAATCTGCA GGATGCTCAG
    AAGTTCATTC TGATGCATGA GGTGGTACAG CCAGTGACCA CAGTGCCCTC CTTCATGGAG
    GACAATAGCC GCTTTTCCCA CGTGGCAGTC GACGTGGTGC AGGGCAGAGA AGCGCTCGTC
    CACATCATCT ATTTGGCCAC AGATTACGGA ACCATTAAGA AAGTGCGGGT ACCCCTGAAT
    CAGACCTCAA GCAGCTGTTT GCTGGAAGAG ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG
    CCCATCAGGA GCCTGCAGAT CCTGCACAGC CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG
    CACGTGGTCA AGATCCCCCT GAAGAGGTGC CAGTTCTACC GCACACGCAG CACCTGCATT
    GGGGCCCAGG ACCCTTACTG TGGCTGGGAT GTGGTAATGA AGAAATGCAC AAGCCTGGAG
    GAGAGCCTGA GCATGACGCA GTGGGAACAG AGCATCTCTG CGTGTCCGAC CAGGAATCTC
    ACCGTGGATG GGCACTTTGG TGTGTGGTCT CCGTGGACGC CTTGCACGCA CACAGATGGC
    AGCGCCGTGG GATCCTGCCT CTGTCGAACC CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT
    GGTGGCTGGC AGTGCGAGGG CCCTGGCATG GAGATCGCCA ACTGTTCCAG GAACGGAGGC
    TGGACTCCCT GGACCTCGTG GTCTCCCTGC AGCACTACCT GTGGGATCGG CTTCCAGGTG
    CGGCAGCGCT CCTGCAGCAA CCCCACTCCC AGGCACGGGG GCCGGGTGTG CGTGGGACAG
    AACCGCGAGG AAAGATACTG CAATGAACAT TTGCTATGTC CCCCACACAT GTTCTGGACA
    GGCTGGGGTC CTTGGGAACG GTGCACAGCC CAATGCGGGG GTGGCATTCA AGCTCGCCGC
    AGGATCTGTG AGAATGGGCC TGACTGTGCA GGCTGCAATG TGGAGTACCA GTCTTGCAAC
    ACCAACCCGT GTCCTGAGCT GAAGAAGACC ACGCCCTGGA CACCCTGGAC ACCTGTCAAC
    ATCTCTGACA ACGGCGGCCA CTATGAGCAA CGATTCCGAT ACACATGCAA AGCCCGCCTG
    GCTGATCCGA ATTTGCTGGA AGTGGGAAGA CAGAGAATCG AAATGCGGTA CTGTTCTAGC
    GACGGCACCA GTGGCTGCTC CACAGATGGG CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA
    TACTCTGCCC ACACGGTCAA CGGGGCTTGG TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC
    CGTGACTGCA GCAGGGGCAT TCGGAACCGG AAGCGTGTTT GCAACAACCC CGAACCCAAG
    TATGGGGGAA TGCCTTGCCT TGGCCCATCT CTGGAATACC AGGAATGCAA CATTTTGCCC
    TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG TCCCCCTGGA CAAAATGTTC AGCAACATGC
    GGCGGTGGAC ACTATATGAG GACCCGCTCT TGCTCCAATC CAGCCCCGGC CTATGGAGGG
    GACATCTGCC TGGGGCTGCA CACAGAAGAG GCACTCTGCA ACACGCAGCC CTGCCCAGAG
    AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC
    CGCCAGTGCA TCCTCCTGTT CCCCATGGGC AGCCAGTGCT CCGGGAACAC CACGGAGAGC
    CGGCCGTGTG TGTTTGACTC TAATTTCATC CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC
    GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC ATGTTCCACA TGATCGCCGT GGGGCTGAGC
    AGCTCCATCC TCGGCTGCCT CCTCACCCTG CTCGTCTATA CTTACTGCCA GCGGTACCAG
    CAGCAATCCC ACGATGCGAC TGTCATCCAC CCCGTCTCAC CTGCCCCCCT TAATACCAGC
    ATAACCAACC ACATCAACAA ACTGGACAAG TACGACTCGG TGGAGGCCAT CAAGGCATTT
    AACAAAAACA ACTTGATCCT AGAGGAAAGA AACAAATACT TCAACCCACA TCTCACTGGG
    AAGACCTATT CTAATGCCTA CTTTACAGAT CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865505.1
    CDS1038..3791
    Translation

    Target ORF information:

    RefSeq Version XM_017010016.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCCCGCT GTCCCTACAG TCCCCAGCAC AATTCCACAG CGCTCCTCAC AGCTGGTGGG 
    GAGCTCTATG CTGCTACAGC CATGGATTTT CCAGGACGTG ATCCTGCCAT TTACCGAAGC
    CTAGGCATTT TACCTCCTCT CCGCACGGCG CAGTACAACT CCAAATGGCT CAATGAGCCA
    AACTTTGTGT CATCTTATGA CATCGGAAAT TTTACCTACT TCTTTTTCCG AGAAAATGCA
    GTAGAGCATG ACTGTGGGAA AACAGTGTTC TCCAGAGCTG CCCGGGTGTG CAAGAACGAT
    ATTGGTGGGC GCTTCCTGCT GGAAGACACC TGGACCACAT TCATGAAGGC TCGCCTGAAC
    TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC TACAACGAAT TGCAGAGTAC TTTCTTCCTG
    CCTGAGCTGG ATTTGATCTA TGGCATCTTT ACCACCAATG TGAACAGCAT TGCGGCCTCA
    GCTGTGTGCG TCTTCAACCT GAGCGCCATC GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC
    CAAGAAAACT CGCGCTCGGC CTGGCTACCG TATCCCAACC CAAACCCCCA CTTCCAGTGT
    GGCACCGTGG ACCAGGGCCT GTACGTGAAC CTGACCGAGA GAAATCTGCA GGATGCTCAG
    AAGTTCATTC TGATGCATGA GGTGGTACAG CCAGTGACCA CAGTGCCCTC CTTCATGGAG
    GACAATAGCC GCTTTTCCCA CGTGGCAGTC GACGTGGTGC AGGGCAGAGA AGCGCTCGTC
    CACATCATCT ATTTGGCCAC AGATTACGGA ACCATTAAGA AAGTGCGGGT ACCCCTGAAT
    CAGACCTCAA GCAGCTGTTT GCTGGAAGAG ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG
    CCCATCAGGA GCCTGCAGAT CCTGCACAGC CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG
    CACGTGGTCA AGATCCCCCT GAAGAGGTGC CAGTTCTACC GCACACGCAG CACCTGCATT
    GGGGCCCAGG ACCCTTACTG TGGCTGGGAT GTGGTAATGA AGAAATGCAC AAGCCTGGAG
    GAGAGCCTGA GCATGACGCA GTGGGAACAG AGCATCTCTG CGTGTCCGAC CAGGAATCTC
    ACCGTGGATG GGCACTTTGG TGTGTGGTCT CCGTGGACGC CTTGCACGCA CACAGATGGC
    AGCGCCGTGG GATCCTGCCT CTGTCGAACC CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT
    GGTGGCTGGC AGTGCGAGGG CCCTGGCATG GAGATCGCCA ACTGTTCCAG GAACGGAGGC
    TGGACTCCCT GGACCTCGTG GTCTCCCTGC AGCACTACCT GTGGGATCGG CTTCCAGGTG
    CGGCAGCGCT CCTGCAGCAA CCCCACTCCC AGGCACGGGG GCCGGGTGTG CGTGGGACAG
    AACCGCGAGG AAAGATACTG CAATGAACAT TTGCTATGTC CCCCACACAT GTTCTGGACA
    GGCTGGGGTC CTTGGGAACG GTGCACAGCC CAATGCGGGG GTGGCATTCA AGCTCGCCGC
    AGGATCTGTG AGAATGGGCC TGACTGTGCA GGCTGCAATG TGGAGTACCA GTCTTGCAAC
    ACCAACCCGT GTCCTGAGCT GAAGAAGACC ACGCCCTGGA CACCCTGGAC ACCTGTCAAC
    ATCTCTGACA ACGGCGGCCA CTATGAGCAA CGATTCCGAT ACACATGCAA AGCCCGCCTG
    GCTGATCCGA ATTTGCTGGA AGTGGGAAGA CAGAGAATCG AAATGCGGTA CTGTTCTAGC
    GACGGCACCA GTGGCTGCTC CACAGATGGG CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA
    TACTCTGCCC ACACGGTCAA CGGGGCTTGG TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC
    CGTGACTGCA GCAGGGGCAT TCGGAACCGG AAGCGTGTTT GCAACAACCC CGAACCCAAG
    TATGGGGGAA TGCCTTGCCT TGGCCCATCT CTGGAATACC AGGAATGCAA CATTTTGCCC
    TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG TCCCCCTGGA CAAAATGTTC AGCAACATGC
    GGCGGTGGAC ACTATATGAG GACCCGCTCT TGCTCCAATC CAGCCCCGGC CTATGGAGGG
    GACATCTGCC TGGGGCTGCA CACAGAAGAG GCACTCTGCA ACACGCAGCC CTGCCCAGAG
    AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC
    CGCCAGTGCA TCCTCCTGTT CCCCATGGGC AGCCAGTGCT CCGGGAACAC CACGGAGAGC
    CGGCCGTGTG TGTTTGACTC TAATTTCATC CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC
    GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC ATGTTCCACA TGATCGCCGT GGGGCTGAGC
    AGCTCCATCC TCGGCTGCCT CCTCACCCTG CTCGTCTATA CTTACTGCCA GCGGTACCAG
    CAGCAATCCC ACGATGCGAC TGTCATCCAC CCCGTCTCAC CTGCCCCCCT TAATACCAGC
    ATAACCAACC ACATCAACAA ACTGGACAAG TACGACTCGG TGGAGGCCAT CAAGGCATTT
    AACAAAAACA ACTTGATCCT AGAGGAAAGA AACAAATACT TCAACCCACA TCTCACTGGG
    AAGACCTATT CTAATGCCTA CTTTACAGAT CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62398
    Clone ID Related Accession (Same CDS sequence) XM_011514159.2
    Accession Version XM_011514159.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGTCACA TATACTCACA GCCTGGAGCA GGAGGACCGC ACACACCACA CAAAGCCACA 
    TGGGAGTCCC GCGGCAGAAG AGTTGAACAT CACAGGGCTG TGGGAGGCAG GCCTTACAGT
    GGAAGGAAGG CTGTGGAATG GGAGTGTGAT GAAGCTACCA AAAAGGCCTG TTACAGCAAA
    GGCAAATCAA AGGAGGAATG TCAGAACTAC ATCCGGGTGC TTCTGGTGGG TGGCGACCGG
    TTATTCACCT GTGGGACCAA TGCATTCACG CCTGTCTGCA CCAACCGCTC GTTGAGCAAC
    CTGACTGAGA TCCATGATCA GATCAGTGGC ATGGCCCGCT GTCCCTACAG TCCCCAGCAC
    AATTCCACAG CGCTCCTCAC AGCTGGTGGG GAGCTCTATG CTGCTACAGC CATGGATTTT
    CCAGGACGTG ATCCTGCCAT TTACCGAAGC CTAGGCATTT TACCTCCTCT CCGCACGGCG
    CAGTACAACT CCAAATGGCT CAATGAGCCA AACTTTGTGT CATCTTATGA CATCGGAAAT
    TTTACCTACT TCTTTTTCCG AGAAAATGCA GTAGAGCATG ACTGTGGGAA AACAGTGTTC
    TCCAGAGCTG CCCGGGTGTG CAAGAACGAT ATTGGTGGGC GCTTCCTGCT GGAAGACACC
    TGGACCACAT TCATGAAGGC TCGCCTGAAC TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC
    TACAACGAAT TGCAGAGTAC TTTCTTCCTG CCTGAGCTGG ATTTGATCTA TGGCATCTTT
    ACCACCAATG TGAACAGCAT TGCGGCCTCA GCTGTGTGCG TCTTCAACCT GAGCGCCATC
    GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC CAAGAAAACT CGCGCTCGGC CTGGCTACCG
    TATCCCAACC CAAACCCCCA CTTCCAGTGT GGCACCGTGG ACCAGGGCCT GTACGTGAAC
    CTGACCGAGA GAAATCTGCA GGATGCTCAG AAGTTCATTC TGATGCATGA GGTGGTACAG
    CCAGTGACCA CAGTGCCCTC CTTCATGGAG GACAATAGCC GCTTTTCCCA CGTGGCAGTC
    GACGTGGTGC AGGGCAGAGA AGCGCTCGTC CACATCATCT ATTTGGCCAC AGATTACGGA
    ACCATTAAGA AAGTGCGGGT ACCCCTGAAT CAGACCTCAA GCAGCTGTTT GCTGGAAGAG
    ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG CCCATCAGGA GCCTGCAGAT CCTGCACAGC
    CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG CACGTGGTCA AGATCCCCCT GAAGAGGTGC
    CAGTTCTACC GCACACGCAG CACCTGCATT GGGGCCCAGG ACCCTTACTG TGGCTGGGAT
    GTGGTAATGA AGAAATGCAC AAGCCTGGAG GAGAGCCTGA GCATGACGCA GTGGGAACAG
    AGCATCTCTG CGTGTCCGAC CAGGAATCTC ACCGTGGATG GGCACTTTGG TGTGTGGTCT
    CCGTGGACGC CTTGCACGCA CACAGATGGC AGCGCCGTGG GATCCTGCCT CTGTCGAACC
    CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT GGTGGCTGGC AGTGCGAGGG CCCTGGCATG
    GAGATCGCCA ACTGTTCCAG GAACGGAGGC TGGACTCCCT GGACCTCGTG GTCTCCCTGC
    AGCACTACCT GTGGGATCGG CTTCCAGGTG CGGCAGCGCT CCTGCAGCAA CCCCACTCCC
    AGGCACGGGG GCCGGGTGTG CGTGGGACAG AACCGCGAGG AAAGATACTG CAATGAACAT
    TTGCTATGTC CCCCACACAT GTTCTGGACA GGCTGGGGTC CTTGGGAACG GTGCACAGCC
    CAATGCGGGG GTGGCATTCA AGCTCGCCGC AGGATCTGTG AGAATGGGCC TGACTGTGCA
    GGCTGCAATG TGGAGTACCA GTCTTGCAAC ACCAACCCGT GTCCTGAGCT GAAGAAGACC
    ACGCCCTGGA CACCCTGGAC ACCTGTCAAC ATCTCTGACA ACGGCGGCCA CTATGAGCAA
    CGATTCCGAT ACACATGCAA AGCCCGCCTG GCTGATCCGA ATTTGCTGGA AGTGGGAAGA
    CAGAGAATCG AAATGCGGTA CTGTTCTAGC GACGGCACCA GTGGCTGCTC CACAGATGGG
    CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA TACTCTGCCC ACACGGTCAA CGGGGCTTGG
    TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC CGTGACTGCA GCAGGGGCAT TCGGAACCGG
    AAGCGTGTTT GCAACAACCC CGAACCCAAG TATGGGGGAA TGCCTTGCCT TGGCCCATCT
    CTGGAATACC AGGAATGCAA CATTTTGCCC TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG
    TCCCCCTGGA CAAAATGTTC AGCAACATGC GGCGGTGGAC ACTATATGAG GACCCGCTCT
    TGCTCCAATC CAGCCCCGGC CTATGGAGGG GACATCTGCC TGGGGCTGCA CACAGAAGAG
    GCACTCTGCA ACACGCAGCC CTGCCCAGAG AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG
    TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC CGCCAGTGCA TCCTCCTGTT CCCCATGGGC
    AGCCAGTGCT CCGGGAACAC CACGGAGAGC CGGCCGTGTG TGTTTGACTC TAATTTCATC
    CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC
    ATGTTCCACA TGATCGCCGT GGGGCTGAGC AGCTCCATCC TCGGCTGCCT CCTCACCCTG
    CTCGTCTATA CTTACTGCCA GCGGTACCAG CAGCAATCCC ACGATGCGAC TGTCATCCAC
    CCCGTCTCAC CTGCCCCCCT TAATACCAGC ATAACCAACC ACATCAACAA ACTGGACAAG
    TACGACTCGG TGGAGGCCAT CAAGGCATTT AACAAAAACA ACTTGATCCT AGAGGAAAGA
    AACAAATACT TCAACCCACA TCTCACTGGG AAGACCTATT CTAATGCCTA CTTTACAGAT
    CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512461.1
    CDS3473..6556
    Translation

    Target ORF information:

    RefSeq Version XM_011514159.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5A (SEMA5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGTCACA TATACTCACA GCCTGGAGCA GGAGGACCGC ACACACCACA CAAAGCCACA 
    TGGGAGTCCC GCGGCAGAAG AGTTGAACAT CACAGGGCTG TGGGAGGCAG GCCTTACAGT
    GGAAGGAAGG CTGTGGAATG GGAGTGTGAT GAAGCTACCA AAAAGGCCTG TTACAGCAAA
    GGCAAATCAA AGGAGGAATG TCAGAACTAC ATCCGGGTGC TTCTGGTGGG TGGCGACCGG
    TTATTCACCT GTGGGACCAA TGCATTCACG CCTGTCTGCA CCAACCGCTC GTTGAGCAAC
    CTGACTGAGA TCCATGATCA GATCAGTGGC ATGGCCCGCT GTCCCTACAG TCCCCAGCAC
    AATTCCACAG CGCTCCTCAC AGCTGGTGGG GAGCTCTATG CTGCTACAGC CATGGATTTT
    CCAGGACGTG ATCCTGCCAT TTACCGAAGC CTAGGCATTT TACCTCCTCT CCGCACGGCG
    CAGTACAACT CCAAATGGCT CAATGAGCCA AACTTTGTGT CATCTTATGA CATCGGAAAT
    TTTACCTACT TCTTTTTCCG AGAAAATGCA GTAGAGCATG ACTGTGGGAA AACAGTGTTC
    TCCAGAGCTG CCCGGGTGTG CAAGAACGAT ATTGGTGGGC GCTTCCTGCT GGAAGACACC
    TGGACCACAT TCATGAAGGC TCGCCTGAAC TGCTCCCGTC CTGGGGAAGT CCCCTTTTAC
    TACAACGAAT TGCAGAGTAC TTTCTTCCTG CCTGAGCTGG ATTTGATCTA TGGCATCTTT
    ACCACCAATG TGAACAGCAT TGCGGCCTCA GCTGTGTGCG TCTTCAACCT GAGCGCCATC
    GCGCAGGCCT TCTCTGGGCC CTTCAAGTAC CAAGAAAACT CGCGCTCGGC CTGGCTACCG
    TATCCCAACC CAAACCCCCA CTTCCAGTGT GGCACCGTGG ACCAGGGCCT GTACGTGAAC
    CTGACCGAGA GAAATCTGCA GGATGCTCAG AAGTTCATTC TGATGCATGA GGTGGTACAG
    CCAGTGACCA CAGTGCCCTC CTTCATGGAG GACAATAGCC GCTTTTCCCA CGTGGCAGTC
    GACGTGGTGC AGGGCAGAGA AGCGCTCGTC CACATCATCT ATTTGGCCAC AGATTACGGA
    ACCATTAAGA AAGTGCGGGT ACCCCTGAAT CAGACCTCAA GCAGCTGTTT GCTGGAAGAG
    ATTGAGCTCT TCCCTGAGAG GCGGAGGGAG CCCATCAGGA GCCTGCAGAT CCTGCACAGC
    CAGAGTGTCC TGTTCGTGGG CCTGCGGGAG CACGTGGTCA AGATCCCCCT GAAGAGGTGC
    CAGTTCTACC GCACACGCAG CACCTGCATT GGGGCCCAGG ACCCTTACTG TGGCTGGGAT
    GTGGTAATGA AGAAATGCAC AAGCCTGGAG GAGAGCCTGA GCATGACGCA GTGGGAACAG
    AGCATCTCTG CGTGTCCGAC CAGGAATCTC ACCGTGGATG GGCACTTTGG TGTGTGGTCT
    CCGTGGACGC CTTGCACGCA CACAGATGGC AGCGCCGTGG GATCCTGCCT CTGTCGAACC
    CGCTCCTGCG ACAGCCCGGC CCCGCAGTGT GGTGGCTGGC AGTGCGAGGG CCCTGGCATG
    GAGATCGCCA ACTGTTCCAG GAACGGAGGC TGGACTCCCT GGACCTCGTG GTCTCCCTGC
    AGCACTACCT GTGGGATCGG CTTCCAGGTG CGGCAGCGCT CCTGCAGCAA CCCCACTCCC
    AGGCACGGGG GCCGGGTGTG CGTGGGACAG AACCGCGAGG AAAGATACTG CAATGAACAT
    TTGCTATGTC CCCCACACAT GTTCTGGACA GGCTGGGGTC CTTGGGAACG GTGCACAGCC
    CAATGCGGGG GTGGCATTCA AGCTCGCCGC AGGATCTGTG AGAATGGGCC TGACTGTGCA
    GGCTGCAATG TGGAGTACCA GTCTTGCAAC ACCAACCCGT GTCCTGAGCT GAAGAAGACC
    ACGCCCTGGA CACCCTGGAC ACCTGTCAAC ATCTCTGACA ACGGCGGCCA CTATGAGCAA
    CGATTCCGAT ACACATGCAA AGCCCGCCTG GCTGATCCGA ATTTGCTGGA AGTGGGAAGA
    CAGAGAATCG AAATGCGGTA CTGTTCTAGC GACGGCACCA GTGGCTGCTC CACAGATGGG
    CTTTCTGGGG ATTTCCTGCG TGCTGGGAGA TACTCTGCCC ACACGGTCAA CGGGGCTTGG
    TCAGCCTGGA CGTCGTGGTC ACAGTGCAGC CGTGACTGCA GCAGGGGCAT TCGGAACCGG
    AAGCGTGTTT GCAACAACCC CGAACCCAAG TATGGGGGAA TGCCTTGCCT TGGCCCATCT
    CTGGAATACC AGGAATGCAA CATTTTGCCC TGCCCAGTGG ATGGCGTGTG GTCTTGCTGG
    TCCCCCTGGA CAAAATGTTC AGCAACATGC GGCGGTGGAC ACTATATGAG GACCCGCTCT
    TGCTCCAATC CAGCCCCGGC CTATGGAGGG GACATCTGCC TGGGGCTGCA CACAGAAGAG
    GCACTCTGCA ACACGCAGCC CTGCCCAGAG AGCTGGTCGG AGTGGTCGGA CTGGTCTGAG
    TGTGAAGCCT CTGGCGTCCA AGTCCGCGCC CGCCAGTGCA TCCTCCTGTT CCCCATGGGC
    AGCCAGTGCT CCGGGAACAC CACGGAGAGC CGGCCGTGTG TGTTTGACTC TAATTTCATC
    CCAGAAGTAT CTGTGGCAAG ATCCAGTAGC GTAGAAGAGA AAAGGTGTGG AGAGTTCAAC
    ATGTTCCACA TGATCGCCGT GGGGCTGAGC AGCTCCATCC TCGGCTGCCT CCTCACCCTG
    CTCGTCTATA CTTACTGCCA GCGGTACCAG CAGCAATCCC ACGATGCGAC TGTCATCCAC
    CCCGTCTCAC CTGCCCCCCT TAATACCAGC ATAACCAACC ACATCAACAA ACTGGACAAG
    TACGACTCGG TGGAGGCCAT CAAGGCATTT AACAAAAACA ACTTGATCCT AGAGGAAAGA
    AACAAATACT TCAACCCACA TCTCACTGGG AAGACCTATT CTAATGCCTA CTTTACAGAT
    CTCAATAATT ATGATGAGTA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.