DPM2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPM2
Entrez Gene ID	8818
Full Name	dolichyl-phosphate mannosyltransferase subunit 2, regulatory
Synonyms	CDG1U
General protein information	Preferred Names dolichyl-phosphate mannosyltransferase subunit 2, regulatory Names dolichol phosphate-mannose biosynthesis regulatory protein DPM synthase complex subunit DPM synthase subunit 2 dolichol-phosphate mannose synthase subunit 2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.11
Summary	Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. [provided by RefSeq, Jul 2008].
Disorder MIM:	603564
Disorder Html:	Congenital disorder of glycosylation, type Iu, 615042 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003863.4	NP_003854.1	dolichol phosphate-mannose biosynthesis regulatory protein
NM_003863.3	NP_003854.1	dolichol phosphate-mannose biosynthesis regulatory protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPM2	XP_003312386.1
Canis lupus familiaris (dog)	DPM2	XP_005625370.1
Gallus gallus (chicken)	DPM2	NP_001186093.1
Oryza sativa (rice)	Os02g0140101	NP_001172795.1
Xenopus tropicalis (tropical clawed frog)	dpm2	NP_001016297.1
	DPM2	XP_001093450.1
Homo sapiens (human)	DPM2	NP_003854.1
Mus musculus (house mouse)	Dpm2	NP_034203.1
Arabidopsis thaliana (thale cress)	AT1G74340	NP_177574.1
Bos taurus (cattle)	DPM2	NP_001039733.1
Rattus norvegicus (Norway rat)	Dpm2	NP_062125.1
Danio rerio (zebrafish)	dpm2	NP_001116318.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of Primary Angle-Closure Glaucoma Susceptibility Loci in Patients with Early Stages of Angle-Closure Disease.
Nongpiur ME, Cheng CY, Duvesh R, Vijayan S, Baskaran M, Khor CC, Allen J, Kavitha S, Venkatesh R, Goh D, Husain R, Boey PY, Quek D, Ho CL, Wong TT, Perera S, Wong TY, Krishnadas SR, Sundaresan P, Aung T, Vithana EN
Ophthalmology(2018 Jan)

DPM2-CDG: a muscular dystrophy-dystroglycanopathy syndrome with severe epilepsy.
Barone R, Aiello C, Race V, Morava E, Foulquier F, Riemersma M, Passarelli C, Concolino D, Carella M, Santorelli F, Vleugels W, Mercuri E, Garozzo D, Sturiale L, Messina S, Jaeken J, Fiumara A, Wevers RA, Bertini E, Matthijs G, Lefeber DJ
Annals of neurology72(4)550-8(2012 Oct)

DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3.
Ashida H, Maeda Y, Kinoshita T
The Journal of biological chemistry281(2)896-904(2006 Jan)

Protein-protein interaction panel using mouse full-length cDNAs.
Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y
Genome research11(10)1758-65(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We describe a new congenital disorders of glycosylation, due to a deficiency of DPM2
Title: DPM2-CDG: a muscular dystrophy-dystroglycanopathy syndrome with severe epilepsy.

The following DPM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21922	NM_003863.4 Latest version!	Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu21922	NM_003863.3	Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPM2 ( NM_003863.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003854.1 Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.

Price & Availability↑

CloneID	OHu21922
Clone ID Related Accession (Same CDS sequence)	NM_003863.4 , NM_003863.3
Accession Version	NM_003863.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	dolichol phosphate-mannose biosynthesis regulatory protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157935.28. On Oct 15, 2019 this sequence version replaced NM_003863.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC015233.1, BU595073.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGG GGACAGACCA GGTGGTGGGA CTCGGCCTCG TCGCCGTTAG CCTGATCATC 
TTCACCTACT ACACCGCCTG GGTGATTCTC TTGCCATTCA TCGACAGTCA GCATGTCATC 
CACAAGTATT TCCTGCCCCG AGCCTATGCT GTCGCCATCC CACTGGCTGC AGGCCTCCTG 
CTGCTCCTGT TTGTGGGACT GTTCATCTCC TATGTGATGC TGAAGACCAA GAGAGTGACC 
AAGAAGGCTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003854.1
CDS	35..289
Translation	MATGTDQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFVGLFISYVMLKTKRVTKKAQ

Target ORF information:

RefSeq Version	NM_003863.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003863.4

ORF Insert Sequence:

ATGGCCACGG GGACAGACCA GGTGGTGGGA CTCGGCCTCG TCGCCGTTAG CCTGATCATC 
TTCACCTACT ACACCGCCTG GGTGATTCTC TTGCCATTCA TCGACAGTCA GCATGTCATC 
CACAAGTATT TCCTGCCCCG AGCCTATGCT GTCGCCATCC CACTGGCTGC AGGCCTCCTG 
CTGCTCCTGT TTGTGGGACT GTTCATCTCC TATGTGATGC TGAAGACCAA GAGAGTGACC 
AAGAAGGCTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPM2 ( NM_003863.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003854.1 Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.

Price & Availability↑

CloneID	OHu21922
Clone ID Related Accession (Same CDS sequence)	NM_003863.4 , NM_003863.3
Accession Version	NM_003863.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	dolichol phosphate-mannose biosynthesis regulatory protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015233.1, AL157935.28 and AI954002.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2009 this sequence version replaced NM_003863.2. ##Evidence-Data-START## Transcript exon combination :: BC015233.1, ERR279846.8949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACGG GGACAGACCA GGTGGTGGGA CTCGGCCTCG TCGCCGTTAG CCTGATCATC 
TTCACCTACT ACACCGCCTG GGTGATTCTC TTGCCATTCA TCGACAGTCA GCATGTCATC 
CACAAGTATT TCCTGCCCCG AGCCTATGCT GTCGCCATCC CACTGGCTGC AGGCCTCCTG 
CTGCTCCTGT TTGTGGGACT GTTCATCTCC TATGTGATGC TGAAGACCAA GAGAGTGACC 
AAGAAGGCTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003854.1
CDS	665..919
Translation	MATGTDQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFVGLFISYVMLKTKRVTKKAQ

Target ORF information:

RefSeq Version	NM_003863.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyl-phosphate mannosyltransferase subunit 2, regulatory (DPM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003863.3

ORF Insert Sequence:

ATGGCCACGG GGACAGACCA GGTGGTGGGA CTCGGCCTCG TCGCCGTTAG CCTGATCATC 
TTCACCTACT ACACCGCCTG GGTGATTCTC TTGCCATTCA TCGACAGTCA GCATGTCATC 
CACAAGTATT TCCTGCCCCG AGCCTATGCT GTCGCCATCC CACTGGCTGC AGGCCTCCTG 
CTGCTCCTGT TTGTGGGACT GTTCATCTCC TATGTGATGC TGAAGACCAA GAGAGTGACC 
AAGAAGGCTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of Primary Angle-Closure Glaucoma Susceptibility Loci in Patients with Early Stages of Angle-Closure Disease.
Ophthalmology(2018 Jan)
Nongpiur ME,Cheng CY,Duvesh R,Vijayan S,Baskaran M,Khor CC,Allen J,Kavitha S,Venkatesh R,Goh D,Husain R,Boey PY,Quek D,Ho CL,Wong TT,Perera S,Wong TY,Krishnadas SR,Sundaresan P,Aung T,Vithana EN

DPM2-CDG: a muscular dystrophy-dystroglycanopathy syndrome with severe epilepsy.
Annals of neurology72(4)550-8(2012 Oct)
Barone R,Aiello C,Race V,Morava E,Foulquier F,Riemersma M,Passarelli C,Concolino D,Carella M,Santorelli F,Vleugels W,Mercuri E,Garozzo D,Sturiale L,Messina S,Jaeken J,Fiumara A,Wevers RA,Bertini E,Matthijs G,Lefeber DJ

DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3.
The Journal of biological chemistry281(2)896-904(2006 Jan)
Ashida H,Maeda Y,Kinoshita T

Protein-protein interaction panel using mouse full-length cDNAs.
Genome research11(10)1758-65(2001 Oct)
Suzuki H,Fukunishi Y,Kagawa I,Saito R,Oda H,Endo T,Kondo S,Bono H,Okazaki Y,Hayashizaki Y

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DPM2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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