ACTN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTN2
Entrez Gene ID	88
Full Name	actinin alpha 2
Synonyms	CMD1AA,CMH23
General protein information	Preferred Names actinin alpha 2 Names alpha-actinin-2 F-actin cross-linking protein alpha-actinin skeletal muscle
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q43
Summary	Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013].
Disorder MIM:	102573
Disorder Html:	Cardiomyopathy, dilated, 1AA, with or without LVNC, 612158 (3); Cardiomyopathy, hypertrophic, 23, with or without LVNC, 612158 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278343.2	NP_001265272.1	alpha-actinin-2 isoform 2
NM_001103.3	NP_001094.1	alpha-actinin-2 isoform 1
NM_001278343.1	NP_001265272.1	alpha-actinin-2 isoform 2
NM_001278344.1	NP_001265273.1	alpha-actinin-2 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACTN2	XP_001158729.2
	ACTN2	XP_001097795.1
Rattus norvegicus (Norway rat)	Actn2	NP_001163796.1
Gallus gallus (chicken)	ACTN2	NP_990654.1
Danio rerio (zebrafish)	actn2	NP_001032662.1
Xenopus tropicalis (tropical clawed frog)	actn2	NP_001005053.1
Homo sapiens (human)	ACTN2	NP_001094.1
Canis lupus familiaris (dog)	ACTN2	XP_856116.1
Bos taurus (cattle)	ACTN2	NP_001029807.1
Mus musculus (house mouse)	Actn2	NP_150371.4

Gene Ontology
Bibliography

Related articles in PubMed

The Interaction of UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE) and Alpha-Actinin 2 Is Altered in GNE Myopathy M743T Mutant.
Harazi A, Becker-Cohen M, Zer H, Moshel O, Hinderlich S, Mitrani-Rosenbaum S
Molecular neurobiology54(4)2928-2938(2017 05)

Distinct subcellular mechanisms for the enhancement of the surface membrane expression of SK2 channel by its interacting proteins, ?-actinin2 and filamin A.
Zhang Z, Ledford HA, Park S, Wang W, Rafizadeh S, Kim HJ, Xu W, Lu L, Lau VC, Knowlton AA, Zhang XD, Yamoah EN, Chiamvimonvat N
The Journal of physiology595(7)2271-2284(2017 Apr)

?-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk.
Grison M, Merkel U, Kostan J, Djinovi?-Carugo K, Rief M
Proceedings of the National Academy of Sciences of the United States of America114(5)1015-1020(2017 Jan)

Hypertrophic cardiomyopathy mutations in the calponin-homology domain of ACTN2 affect actin binding and cardiomyocyte Z-disc incorporation.
Haywood NJ, Wolny M, Rogers B, Trinh CH, Shuping Y, Edwards TA, Peckham M
The Biochemical journal473(16)2485-93(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the interaction between GNE and alpha-actinin 1 and alpha-actinin 2 occur at different sites in the alpha-actinin molecules and that for alpha-actinin 2 the interaction site is located at the C-terminus of the protein.
Title: The Interaction of UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE) and Alpha-Actinin 2 Is Altered in GNE Myopathy M743T Mutant.

These results provide new insights into the regulation of SK2 channel trafficking by the cytoskeletal proteins FLNA and alpha-actinin2, involving distinct recycling pathways
Title: Distinct subcellular mechanisms for the enhancement of the surface membrane expression of SK2 channel by its interacting proteins, ?-actinin2 and filamin A.

The full length mEos2 tagged protein expressed in adult cardiomyocytes shows that both mutations additionally affect Z-disc localization and dynamic behaviour
Title: Hypertrophic cardiomyopathy mutations in the calponin-homology domain of ACTN2 affect actin binding and cardiomyocyte Z-disc incorporation.

Study reports a complete high-resolution structure of the 200 kDa alpha-actinin-2 dimer from striated muscle and explore its functional implications on the biochemical and cellular level.
Title: The structure and regulation of human muscle ?-actinin.

Clinical evaluation of an Australian family revealed diverse cardiac pathologies in four affected members and genetic testing of the exome identified a pathogenic ACTN2 heterozygous variant (Ala119Thr) that co-segregated with disease.
Title: Exome sequencing identifies a mutation in the ACTN2 gene in a family with idiopathic ventricular fibrillation, left ventricular noncompaction, and sudden death.

The following ACTN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22647	NM_001103.3 Latest version!	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22707	NM_001278344.1 Latest version!	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu23009	NM_001278343.1 Latest version!	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu108409	NM_001278343.2 Latest version!	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTN2 ( NM_001103.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001094.1 Homo sapiens actinin alpha 2 (ACTN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22647
Clone ID Related Accession (Same CDS sequence)	NM_001103.3
Accession Version	NM_001103.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	alpha-actinin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315250.1, BC047901.2, AL359185.25 and BX103017.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2013 this sequence version replaced NM_001103.2. Transcript Variant: This variant (1) encodes one of the longer isoforms (1) of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.84390.1, SRR1803612.90948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT CGGCGCTGAA 
GAAATTGTTG ATGGCAATGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGACA TCGTGAACAC CCCTAAACCC 
GATGAAAGAG CCATCATGAC GTACGTCTCT TGCTTCTACC ACGCTTTTGC GGGCGCGGAG 
CAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094.1
CDS	221..2905
Translation	MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_001103.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103.3

ORF Insert Sequence:

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT CGGCGCTGAA 
GAAATTGTTG ATGGCAATGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGACA TCGTGAACAC CCCTAAACCC 
GATGAAAGAG CCATCATGAC GTACGTCTCT TGCTTCTACC ACGCTTTTGC GGGCGCGGAG 
CAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN2 ( NM_001278344.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265273.1 Homo sapiens actinin alpha 2 (ACTN2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22707
Clone ID Related Accession (Same CDS sequence)	NM_001278344.1
Accession Version	NM_001278344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	alpha-actinin-2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315250.1, AB209521.1, AL359185.25 and BX103017.1. Transcript Variant: This variant (3) contains two alternate internal exons compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGTACG TCTCTTGCTT CTACCACGCT TTTGCGGGCG CGGAGCAGGT TAGACAAAGT 
CTTAAAGCAC ACTCAGCTCT GTGGAAGGAT CCCCCTCCAG AAAGTTCTAC ATGTTCATAT 
CAGGAGATGA GGAGGTCTTC AGTGAATTCA AGTGCAATGG CCGAGACAGC GGCTAACAGG 
ATATGTAAGG TTCTTGCTGT GAATCAAGAG AATGAGAGGC TGATGGAAGA ATATGAGAGG 
CTAGCGAGTG AGCTTTTGGA ATGGATTCGT CGCACGATCC CCTGGCTGGA GAACCGGACT 
CCCGAGAAGA CCATGCAAGC CATGCAGAAG AAGCTGGAGG ACTTCCGGGA TTACCGCCGG 
AAGCACAAGC CACCCAAGGT GCAGGAGAAA TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGATCAG CAACCGTCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG 
TCGGATATTG CTGGTGCCTG GCAGAGGCTG GAGCAGGCTG AGAAGGGTTA CGAGGAGTGG 
TTGCTCAATG AGATTCGGAG ACTGGAGCGC TTGGAACACC TGGCTGAGAA GTTCAGGCAG 
AAGGCCTCAA CGCACGAGAC TTGGGCTTAT GGCAAAGAGC AGATCTTGCT GCAGAAGGAT 
TACGAGTCGG CGTCGCTGAC AGAGGTGCGG GCTCTGCTGC GGAAGCACGA GGCGTTCGAG 
AGCGACCTGG CAGCGCACCA GGACCGCGTG GAGCAGATCG CAGCCATCGC GCAGGAGCTC 
AATGAACTGG ACTATCACGA CGCTGTGAAT GTCAATGATC GGTGCCAGAA AATTTGTGAC 
CAGTGGGACC GACTGGGAAC GCTTACTCAG AAGAGGAGAG AAGCCCTAGA GAGAATGGAG 
AAATTGCTAG AAACCATTGA TCAGCTTCAC CTGGAGTTTG CCAAGAGGGC TGCTCCTTTC 
AACAATTGGA TGGAGGGCGC TATGGAGGAT CTGCAAGATA TGTTCATTGT CCACAGCATT 
GAGGAGATCC AGAGTCTGAT CACTGCGCAT GAGCAGTTCA AGGCCACGCT GCCCGAGGCG 
GACGGAGAGC GGCAGTCCAT CATGGCCATC CAGAACGAGG TGGAGAAGGT GATTCAGAGC 
TACAACATCA GAATCAGCTC AAGCAACCCG TACAGCACTG TCACCATGGA TGAGCTCCGG 
ACCAAGTGGG ACAAGGTGAA GCAACTCGTG CCCATCCGCG ATCAATCCCT GCAGGAGGAG 
CTGGCTCGCC AGCATGCTAA CGAGCGTCTG AGGCGCCAGT TTGCTGCCCA AGCCAATGCC 
ATTGGGCCCT GGATCCAGAA CAAGATGGAG GAGATTGCCC GGAGCTCCAT CCAGATCACA 
GGAGCCCTGG AAGACCAGAT GAACCAGCTG AAGCAGTATG AGCACAACAT CATCAACTAT 
AAGAACAACA TCGACAAGCT GGAGGGAGAC CATCAGCTCA TCCAGGAGGC CCTTGTCTTT 
GACAACAAGC ACACGAACTA CACGATGGAG CACATTCGTG TTGGATGGGA GCTGCTGCTG 
ACAACCATCG CCAGAACCAT CAATGAGGTG GAGACTCAGA TCCTGACGAG AGATGCGAAG 
GGCATCACCC AGGAGCAGAT GAATGAGTTC AGAGCCTCCT TCAACCACTT TGACAGGAGG 
AAGAATGGCC TGATGGATCA TGAGGATTTC AGAGCCTGCC TGATTTCCAT GGGTTATGAC 
CTGGGTGAAG CCGAATTTGC CCGCATTATG ACCCTGGTAG ATCCCAACGG GCAAGGCACC 
GTCACCTTCC AATCCTTCAT CGACTTCATG ACTAGAGAGA CGGCTGACAC CGACACTGCC 
GAGCAGGTCA TCGCCTCCTT CCGGATCCTG GCTTCTGATA AGCCATACAT CCTGGCGGAG 
GAGCTGCGTC GGGAGCTGCC CCCGGATCAG GCCCAGTACT GCATCAAGAG GATGCCCGCC 
TACTCGGGCC CAGGCAGTGT GCCTGGTGCA CTGGATTACG CTGCGTTCTC TTCCGCACTC 
TACGGGGAGA GCGATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265273.1
CDS	1042..3102
Translation	MTYVSCFYHAFAGAEQVRQSLKAHSALWKDPPPESSTCSYQEMRRSSVNSSAMAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_001278344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278344.1

ORF Insert Sequence:

ATGACGTACG TCTCTTGCTT CTACCACGCT TTTGCGGGCG CGGAGCAGGT TAGACAAAGT 
CTTAAAGCAC ACTCAGCTCT GTGGAAGGAT CCCCCTCCAG AAAGTTCTAC ATGTTCATAT 
CAGGAGATGA GGAGGTCTTC AGTGAATTCA AGTGCAATGG CCGAGACAGC GGCTAACAGG 
ATATGTAAGG TTCTTGCTGT GAATCAAGAG AATGAGAGGC TGATGGAAGA ATATGAGAGG 
CTAGCGAGTG AGCTTTTGGA ATGGATTCGT CGCACGATCC CCTGGCTGGA GAACCGGACT 
CCCGAGAAGA CCATGCAAGC CATGCAGAAG AAGCTGGAGG ACTTCCGGGA TTACCGCCGG 
AAGCACAAGC CACCCAAGGT GCAGGAGAAA TGCCAGCTGG AGATCAACTT CAACACGCTG 
CAGACCAAGC TGCGGATCAG CAACCGTCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG 
TCGGATATTG CTGGTGCCTG GCAGAGGCTG GAGCAGGCTG AGAAGGGTTA CGAGGAGTGG 
TTGCTCAATG AGATTCGGAG ACTGGAGCGC TTGGAACACC TGGCTGAGAA GTTCAGGCAG 
AAGGCCTCAA CGCACGAGAC TTGGGCTTAT GGCAAAGAGC AGATCTTGCT GCAGAAGGAT 
TACGAGTCGG CGTCGCTGAC AGAGGTGCGG GCTCTGCTGC GGAAGCACGA GGCGTTCGAG 
AGCGACCTGG CAGCGCACCA GGACCGCGTG GAGCAGATCG CAGCCATCGC GCAGGAGCTC 
AATGAACTGG ACTATCACGA CGCTGTGAAT GTCAATGATC GGTGCCAGAA AATTTGTGAC 
CAGTGGGACC GACTGGGAAC GCTTACTCAG AAGAGGAGAG AAGCCCTAGA GAGAATGGAG 
AAATTGCTAG AAACCATTGA TCAGCTTCAC CTGGAGTTTG CCAAGAGGGC TGCTCCTTTC 
AACAATTGGA TGGAGGGCGC TATGGAGGAT CTGCAAGATA TGTTCATTGT CCACAGCATT 
GAGGAGATCC AGAGTCTGAT CACTGCGCAT GAGCAGTTCA AGGCCACGCT GCCCGAGGCG 
GACGGAGAGC GGCAGTCCAT CATGGCCATC CAGAACGAGG TGGAGAAGGT GATTCAGAGC 
TACAACATCA GAATCAGCTC AAGCAACCCG TACAGCACTG TCACCATGGA TGAGCTCCGG 
ACCAAGTGGG ACAAGGTGAA GCAACTCGTG CCCATCCGCG ATCAATCCCT GCAGGAGGAG 
CTGGCTCGCC AGCATGCTAA CGAGCGTCTG AGGCGCCAGT TTGCTGCCCA AGCCAATGCC 
ATTGGGCCCT GGATCCAGAA CAAGATGGAG GAGATTGCCC GGAGCTCCAT CCAGATCACA 
GGAGCCCTGG AAGACCAGAT GAACCAGCTG AAGCAGTATG AGCACAACAT CATCAACTAT 
AAGAACAACA TCGACAAGCT GGAGGGAGAC CATCAGCTCA TCCAGGAGGC CCTTGTCTTT 
GACAACAAGC ACACGAACTA CACGATGGAG CACATTCGTG TTGGATGGGA GCTGCTGCTG 
ACAACCATCG CCAGAACCAT CAATGAGGTG GAGACTCAGA TCCTGACGAG AGATGCGAAG 
GGCATCACCC AGGAGCAGAT GAATGAGTTC AGAGCCTCCT TCAACCACTT TGACAGGAGG 
AAGAATGGCC TGATGGATCA TGAGGATTTC AGAGCCTGCC TGATTTCCAT GGGTTATGAC 
CTGGGTGAAG CCGAATTTGC CCGCATTATG ACCCTGGTAG ATCCCAACGG GCAAGGCACC 
GTCACCTTCC AATCCTTCAT CGACTTCATG ACTAGAGAGA CGGCTGACAC CGACACTGCC 
GAGCAGGTCA TCGCCTCCTT CCGGATCCTG GCTTCTGATA AGCCATACAT CCTGGCGGAG 
GAGCTGCGTC GGGAGCTGCC CCCGGATCAG GCCCAGTACT GCATCAAGAG GATGCCCGCC 
TACTCGGGCC CAGGCAGTGT GCCTGGTGCA CTGGATTACG CTGCGTTCTC TTCCGCACTC 
TACGGGGAGA GCGATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN2 ( NM_001278343.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265272.1 Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23009
Clone ID Related Accession (Same CDS sequence)	NM_001278343.1
Accession Version	NM_001278343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	alpha-actinin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315250.1, AL359185.25 and BX103017.1. Transcript Variant: This variant (2) lacks an alternate internal coding exon and contains another compared to variant 1. The resulting isoform (2) has a few internal differences but is the same length as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315250.1, SRR1803616.266488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT CGGCGCTGAA 
GAAATTGTTG ATGGCAATGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGATT TAGTATACAC TGCCAGACCC 
GATGAAAGAG CCATAATGAC TTATGTTTCC TGTTACTATC ATGCTTTTGC TGGTGCACAG 
AAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265272.1
CDS	221..2905
Translation	MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDLVYTARPDERAIMTYVSCYYHAFAGAQKAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_001278343.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278343.1

ORF Insert Sequence:

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT CGGCGCTGAA 
GAAATTGTTG ATGGCAATGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGATT TAGTATACAC TGCCAGACCC 
GATGAAAGAG CCATAATGAC TTATGTTTCC TGTTACTATC ATGCTTTTGC TGGTGCACAG 
AAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTN2 ( NM_001278343.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265272.1 Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108409
Clone ID Related Accession (Same CDS sequence)	NM_001278343.2
Accession Version	NM_001278343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	alpha-actinin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359921.13 and AL359185.25. On Jun 2, 2019 this sequence version replaced NM_001278343.1. Transcript Variant: This variant (2) lacks an alternate internal coding exon and contains another compared to variant 1. The resulting isoform (2) has a few internal differences but is the same length as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.71471.1, SRR1803616.266488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT TGGCGCTGAA 
GAAATTGTTG ATGGCAACGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGATT TAGTATACAC TGCCAGACCC 
GATGAAAGAG CCATAATGAC TTATGTTTCC TGTTACTATC ATGCTTTTGC TGGTGCACAG 
AAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265272.1
CDS	176..2860
Translation	MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDLVYTARPDERAIMTYVSCYYHAFAGAQKAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_001278343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actinin alpha 2 (ACTN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278343.2

ORF Insert Sequence:

ATGAACCAGA TAGAGCCCGG CGTGCAGTAC AACTACGTGT ACGACGAGGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTGGACC CAGCCTGGGA GAAGCAGCAG 
AGGAAGACCT TCACTGCCTG GTGTAACTCC CACCTAAGGA AAGCCGGCAC CCAGATTGAG 
AACATCGAGG AAGACTTCAG GAATGGCCTT AAGCTCATGC TGCTTTTGGA AGTCATCTCA 
GGGGAAAGGC TGCCCAAACC TGACCGGGGA AAAATGCGGT TCCACAAAAT TGCTAATGTC 
AACAAAGCTT TGGATTACAT AGCCAGCAAA GGGGTGAAAC TGGTGTCCAT TGGCGCTGAA 
GAAATTGTTG ATGGCAACGT GAAAATGACC CTGGGTATGA TCTGGACCAT CATCCTTCGC 
TTTGCTATTC AGGATATTTC GGTTGAAGAA ACATCTGCCA AAGAAGGTCT GCTGCTTTGG 
TGTCAGAGGA AAACTGCTCC TTATAGAAAT GTGAACATTC AGAACTTCCA TACTAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACCGACACC GGCCTGACCT CATTGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AATATTAACC TGGCCATGGA AATCGCTGAG 
AAGCACCTGG ATATTCCTAA AATGTTGGAT GCTGAAGATT TAGTATACAC TGCCAGACCC 
GATGAAAGAG CCATAATGAC TTATGTTTCC TGTTACTATC ATGCTTTTGC TGGTGCACAG 
AAGGCCGAGA CAGCGGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTTT TGGAATGGAT TCGTCGCACG 
ATCCCCTGGC TGGAGAACCG GACTCCCGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGATTACCG CCGGAAGCAC AAGCCACCCA AGGTGCAGGA GAAATGCCAG 
CTGGAGATCA ACTTCAACAC GCTGCAGACC AAGCTGCGGA TCAGCAACCG TCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAT ATTGCTGGTG CCTGGCAGAG GCTGGAGCAG 
GCTGAGAAGG GTTACGAGGA GTGGTTGCTC AATGAGATTC GGAGACTGGA GCGCTTGGAA 
CACCTGGCTG AGAAGTTCAG GCAGAAGGCC TCAACGCACG AGACTTGGGC TTATGGCAAA 
GAGCAGATCT TGCTGCAGAA GGATTACGAG TCGGCGTCGC TGACAGAGGT GCGGGCTCTG 
CTGCGGAAGC ACGAGGCGTT CGAGAGCGAC CTGGCAGCGC ACCAGGACCG CGTGGAGCAG 
ATCGCAGCCA TCGCGCAGGA GCTCAATGAA CTGGACTATC ACGACGCTGT GAATGTCAAT 
GATCGGTGCC AGAAAATTTG TGACCAGTGG GACCGACTGG GAACGCTTAC TCAGAAGAGG 
AGAGAAGCCC TAGAGAGAAT GGAGAAATTG CTAGAAACCA TTGATCAGCT TCACCTGGAG 
TTTGCCAAGA GGGCTGCTCC TTTCAACAAT TGGATGGAGG GCGCTATGGA GGATCTGCAA 
GATATGTTCA TTGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACTGC GCATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCGGACGGA GAGCGGCAGT CCATCATGGC CATCCAGAAC 
GAGGTGGAGA AGGTGATTCA GAGCTACAAC ATCAGAATCA GCTCAAGCAA CCCGTACAGC 
ACTGTCACCA TGGATGAGCT CCGGACCAAG TGGGACAAGG TGAAGCAACT CGTGCCCATC 
CGCGATCAAT CCCTGCAGGA GGAGCTGGCT CGCCAGCATG CTAACGAGCG TCTGAGGCGC 
CAGTTTGCTG CCCAAGCCAA TGCCATTGGG CCCTGGATCC AGAACAAGAT GGAGGAGATT 
GCCCGGAGCT CCATCCAGAT CACAGGAGCC CTGGAAGACC AGATGAACCA GCTGAAGCAG 
TATGAGCACA ACATCATCAA CTATAAGAAC AACATCGACA AGCTGGAGGG AGACCATCAG 
CTCATCCAGG AGGCCCTTGT CTTTGACAAC AAGCACACGA ACTACACGAT GGAGCACATT 
CGTGTTGGAT GGGAGCTGCT GCTGACAACC ATCGCCAGAA CCATCAATGA GGTGGAGACT 
CAGATCCTGA CGAGAGATGC GAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTGATTT CCATGGGTTA TGACCTGGGT GAAGCCGAAT TTGCCCGCAT TATGACCCTG 
GTAGATCCCA ACGGGCAAGG CACCGTCACC TTCCAATCCT TCATCGACTT CATGACTAGA 
GAGACGGCTG ACACCGACAC TGCCGAGCAG GTCATCGCCT CCTTCCGGAT CCTGGCTTCT 
GATAAGCCAT ACATCCTGGC GGAGGAGCTG CGTCGGGAGC TGCCCCCGGA TCAGGCCCAG 
TACTGCATCA AGAGGATGCC CGCCTACTCG GGCCCAGGCA GTGTGCCTGG TGCACTGGAT 
TACGCTGCGT TCTCTTCCGC ACTCTACGGG GAGAGCGATC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Interaction of UDP-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase (GNE) and Alpha-Actinin 2 Is Altered in GNE Myopathy M743T Mutant.
Molecular neurobiology54(4)2928-2938(2017 05)
Harazi A,Becker-Cohen M,Zer H,Moshel O,Hinderlich S,Mitrani-Rosenbaum S

Distinct subcellular mechanisms for the enhancement of the surface membrane expression of SK2 channel by its interacting proteins, ?-actinin2 and filamin A.
The Journal of physiology595(7)2271-2284(2017 Apr)
Zhang Z,Ledford HA,Park S,Wang W,Rafizadeh S,Kim HJ,Xu W,Lu L,Lau VC,Knowlton AA,Zhang XD,Yamoah EN,Chiamvimonvat N

?-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk.
Proceedings of the National Academy of Sciences of the United States of America114(5)1015-1020(2017 Jan)
Grison M,Merkel U,Kostan J,Djinovi?-Carugo K,Rief M

Hypertrophic cardiomyopathy mutations in the calponin-homology domain of ACTN2 affect actin binding and cardiomyocyte Z-disc incorporation.
The Biochemical journal473(16)2485-93(2016 08)
Haywood NJ,Wolny M,Rogers B,Trinh CH,Shuping Y,Edwards TA,Peckham M

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ACTN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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