SEMA7A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA7A
Entrez Gene ID	8482
Full Name	semaphorin 7A (John Milton Hagen blood group)
Synonyms	CD108,CDw108,H-SEMA-K1,H-Sema-L,JMH,SEMAK1,SEMAL
General protein information	Preferred Names semaphorin 7A (John Milton Hagen blood group) Names semaphorin-7A JMH blood group antigen John Milton Hagen blood group H-Sema K1 john-Milton-Hargen human blood group Ag sema K1 sema L sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group) sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) semaphorin-K1 semaphorin-L
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.1
Summary	This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016].
Disorder MIM:	607961
Disorder Html:	[Blood group, John-Milton-Hagen system], 614745 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001146029.3	NP_001139501.1	semaphorin-7A isoform 2 precursor
NM_001146030.2	NP_001139502.1	semaphorin-7A isoform 3
NM_003612.5	NP_003603.1	semaphorin-7A isoform 1 preproprotein
NM_001146029.2	NP_001139501.1	semaphorin-7A isoform 2 precursor
NM_003612.4	NP_003603.1	semaphorin-7A isoform 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SEMA7A	NP_001193114.1
Homo sapiens (human)	SEMA7A	NP_003603.1
Gallus gallus (chicken)	SEMA7A	NP_001186678.1
Danio rerio (zebrafish)	sema7a	NP_001108357.1
	SEMA7A	XP_001099042.1
Canis lupus familiaris (dog)	SEMA7A	XP_005638673.1
Rattus norvegicus (Norway rat)	Sema7a	NP_001101623.1
Pan troglodytes (chimpanzee)	SEMA7A	XP_523219.2
Mus musculus (house mouse)	Sema7a	NP_035482.1
Xenopus tropicalis (tropical clawed frog)	sema7a	XP_002937527.2

Gene Ontology
Bibliography

Related articles in PubMed

Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial ?1 Integrin.
Hu S, Liu Y, You T, Heath J, Xu L, Zheng X, Wang A, Wang Y, Li F, Yang F, Cao Y, Zhang H, van Gils JM, van Zonneveld AJ, Jo H, Wu Q, Zhang Y, Tang C, Zhu L
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)

Identification and Evaluation of New Immunoregulatory Genes in Mesenchymal Stromal Cells of Different Origins: Comparison of Normal and Inflammatory Conditions.
Fayyad-Kazan M, Najar M, Fayyad-Kazan H, Raicevic G, Lagneaux L
Medical science monitor basic research2387-96(2017 Mar)

Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression.
Scott GA, McClelland LA, Fricke AF, Fender A
The Journal of investigative dermatology129(4)954-63(2009 Apr)

Semaphorin 7a promotes spreading and dendricity in human melanocytes through beta1-integrins.
Scott GA, McClelland LA, Fricke AF
The Journal of investigative dermatology128(1)151-61(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results identified FGL2, GAL, SEMA4D, SEMA7A, and IDO1 as new candidate genes that could be involved in MSCs-mediated immunomodulation. FGL2, GAL, SEMA4D, SEMA7A, and IDO1 genes appeared to be differentially transcribed in the different MSC populations. Moreover, these genes were not similarly modulated following MSCs-exposure to inflammatory signals
Title: Identification and Evaluation of New Immunoregulatory Genes in Mesenchymal Stromal Cells of Different Origins: Comparison of Normal and Inflammatory Conditions.

These findings indicate that Sema7A as a potent activator of T cells and monocytes in the immune response contributes to the inflammation and progression of rheumatoid arthritis
Title: Semaphorin 7A as a potential immune regulator and promising therapeutic target in rheumatoid arthritis.

short hairpin-mediated silencing of SEMA7A reveals roles for semaphorin 7a in the promotion of DCIS growth, motility and invasion as well as lymphangiogenesis in the tumor microenvironment. Our studies also uncover a relationship between COX-2 and semaphorin 7a expression and suggest that semaphorin 7a promotes tumor cell invasion on collagen and lymphangiogenesis via activation of b1-integrin receptor
Title: Semaphorin 7a exerts pleiotropic effects to promote breast tumor progression.

This study demonstrates that Sema7A controls the assembly of actin-based protrusions that drive Dendritic cell migration in response to CCL21.
Title: Semaphorin 7A Promotes Chemokine-Driven Dendritic Cell Migration.

SEMA7A has a role in the development of lung injury
Title: Semaphorin 7A Aggravates Pulmonary Inflammation during Lung Injury.

The following SEMA7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23073	NM_003612.5 Latest version!	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu12063	NM_001146030.2 Latest version!	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu10551	NM_001146029.3 Latest version!	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
View Old Accession Versions >>
OHu10551	NM_001146029.2	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu23073	NM_003612.4	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA7A ( NM_003612.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003603.1 Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23073
Clone ID Related Accession (Same CDS sequence)	NM_003612.5 , NM_003612.4
Accession Version	NM_003612.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	semaphorin-7A isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312257.1, AK001895.1, AC090826.15, BF792612.1 and AI972833.1. On Nov 22, 2018 this sequence version replaced NM_003612.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312257.1, AF069493.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261918.9/ ENSP00000261918.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GTGAATATCG GCTCCACAAA GGGGTCCTGT 
CTGGATAAGC GGGACTGCGA GAACTACATC ACTCTCCTGG AGAGGCGGAG TGAGGGGCTG 
CTGGCCTGTG GCACCAACGC CCGGCACCCC AGCTGCTGGA ACCTGGTGAA TGGCACTGTG 
GTGCCACTTG GCGAGATGAG AGGCTACGCC CCCTTCAGCC CGGACGAGAA CTCCCTGGTT 
CTGTTTGAAG GGGACGAGGT GTATTCCACC ATCCGGAAGC AGGAATACAA TGGGAAGATC 
CCTCGGTTCC GCCGCATCCG GGGCGAGAGT GAGCTGTACA CCAGTGATAC TGTCATGCAG 
AACCCACAGT TCATCAAAGC CACCATCGTG CACCAAGACC AGGCTTACGA TGACAAGATC 
TACTACTTCT TCCGAGAGGA CAATCCTGAC AAGAATCCTG AGGCTCCTCT CAATGTGTCC 
CGTGTGGCCC AGTTGTGCAG GGGGGACCAG GGTGGGGAAA GTTCACTGTC AGTCTCCAAG 
TGGAACACTT TTCTGAAAGC CATGCTGGTA TGCAGTGATG CTGCCACCAA CAAGAACTTC 
AACAGGCTGC AAGACGTCTT CCTGCTCCCT GACCCCAGCG GCCAGTGGAG GGACACCAGG 
GTCTATGGTG TTTTCTCCAA CCCCTGGAAC TACTCAGCCG TCTGTGTGTA TTCCCTCGGT 
GACATTGACA AGGTCTTCCG TACCTCCTCA CTCAAGGGCT ACCACTCAAG CCTTCCCAAC 
CCGCGGCCTG GCAAGTGCCT CCCAGACCAG CAGCCGATAC CCACAGAGAC CTTCCAGGTG 
GCTGACCGTC ACCCAGAGGT GGCGCAGAGG GTGGAGCCCA TGGGGCCTCT GAAGACGCCA 
TTGTTCCACT CTAAATACCA CTACCAGAAA GTGGCCGTCC ACCGCATGCA AGCCAGCCAC 
GGGGAGACCT TTCATGTGCT TTACCTAACT ACAGACAGGG GCACTATCCA CAAGGTGGTG 
GAACCGGGGG AGCAGGAGCA CAGCTTCGCC TTCAACATCA TGGAGATCCA GCCCTTCCGC 
CGCGCGGCTG CCATCCAGAC CATGTCGCTG GATGCTGAGC GGAGGAAGCT GTATGTGAGC 
TCCCAGTGGG AGGTGAGCCA GGTGCCCCTG GACCTGTGTG AGGTCTATGG CGGGGGCTGC 
CACGGTTGCC TCATGTCCCG AGACCCCTAC TGCGGCTGGG ACCAAGGCCG CTGCATCTCC 
ATCTACAGCT CCGAACGGTC AGTGCTGCAA TCCATTAATC CAGCCGAGCC ACACAAGGAG 
TGTCCCAACC CCAAACCAGA CAAGGCCCCA CTGCAGAAGG TTTCCCTGGC CCCAAACTCT 
CGCTACTACC TGAGCTGCCC CATGGAATCC CGCCACGCCA CCTACTCATG GCGCCACAAG 
GAGAACGTGG AGCAGAGCTG CGAACCTGGT CACCAGAGCC CCAACTGCAT CCTGTTCATC 
GAGAACCTCA CGGCGCAGCA GTACGGCCAC TACTTCTGCG AGGCCCAGGA GGGCTCCTAC 
TTCCGCGAGG CTCAGCACTG GCAGCTGCTG CCCGAGGACG GCATCATGGC CGAGCACCTG 
CTGGGTCATG CCTGTGCCCT GGCCGCCTCC CTCTGGCTGG GGGTGCTGCC CACACTCACT 
CTTGGCTTGC TGGTCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003603.1
CDS	41..2041
Translation	MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH

Target ORF information:

RefSeq Version	NM_003612.5
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003612.5

ORF Insert Sequence:

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GTGAATATCG GCTCCACAAA GGGGTCCTGT 
CTGGATAAGC GGGACTGCGA GAACTACATC ACTCTCCTGG AGAGGCGGAG TGAGGGGCTG 
CTGGCCTGTG GCACCAACGC CCGGCACCCC AGCTGCTGGA ACCTGGTGAA TGGCACTGTG 
GTGCCACTTG GCGAGATGAG AGGCTACGCC CCCTTCAGCC CGGACGAGAA CTCCCTGGTT 
CTGTTTGAAG GGGACGAGGT GTATTCCACC ATCCGGAAGC AGGAATACAA TGGGAAGATC 
CCTCGGTTCC GCCGCATCCG GGGCGAGAGT GAGCTGTACA CCAGTGATAC TGTCATGCAG 
AACCCACAGT TCATCAAAGC CACCATCGTG CACCAAGACC AGGCTTACGA TGACAAGATC 
TACTACTTCT TCCGAGAGGA CAATCCTGAC AAGAATCCTG AGGCTCCTCT CAATGTGTCC 
CGTGTGGCCC AGTTGTGCAG GGGGGACCAG GGTGGGGAAA GTTCACTGTC AGTCTCCAAG 
TGGAACACTT TTCTGAAAGC CATGCTGGTA TGCAGTGATG CTGCCACCAA CAAGAACTTC 
AACAGGCTGC AAGACGTCTT CCTGCTCCCT GACCCCAGCG GCCAGTGGAG GGACACCAGG 
GTCTATGGTG TTTTCTCCAA CCCCTGGAAC TACTCAGCCG TCTGTGTGTA TTCCCTCGGT 
GACATTGACA AGGTCTTCCG TACCTCCTCA CTCAAGGGCT ACCACTCAAG CCTTCCCAAC 
CCGCGGCCTG GCAAGTGCCT CCCAGACCAG CAGCCGATAC CCACAGAGAC CTTCCAGGTG 
GCTGACCGTC ACCCAGAGGT GGCGCAGAGG GTGGAGCCCA TGGGGCCTCT GAAGACGCCA 
TTGTTCCACT CTAAATACCA CTACCAGAAA GTGGCCGTCC ACCGCATGCA AGCCAGCCAC 
GGGGAGACCT TTCATGTGCT TTACCTAACT ACAGACAGGG GCACTATCCA CAAGGTGGTG 
GAACCGGGGG AGCAGGAGCA CAGCTTCGCC TTCAACATCA TGGAGATCCA GCCCTTCCGC 
CGCGCGGCTG CCATCCAGAC CATGTCGCTG GATGCTGAGC GGAGGAAGCT GTATGTGAGC 
TCCCAGTGGG AGGTGAGCCA GGTGCCCCTG GACCTGTGTG AGGTCTATGG CGGGGGCTGC 
CACGGTTGCC TCATGTCCCG AGACCCCTAC TGCGGCTGGG ACCAAGGCCG CTGCATCTCC 
ATCTACAGCT CCGAACGGTC AGTGCTGCAA TCCATTAATC CAGCCGAGCC ACACAAGGAG 
TGTCCCAACC CCAAACCAGA CAAGGCCCCA CTGCAGAAGG TTTCCCTGGC CCCAAACTCT 
CGCTACTACC TGAGCTGCCC CATGGAATCC CGCCACGCCA CCTACTCATG GCGCCACAAG 
GAGAACGTGG AGCAGAGCTG CGAACCTGGT CACCAGAGCC CCAACTGCAT CCTGTTCATC 
GAGAACCTCA CGGCGCAGCA GTACGGCCAC TACTTCTGCG AGGCCCAGGA GGGCTCCTAC 
TTCCGCGAGG CTCAGCACTG GCAGCTGCTG CCCGAGGACG GCATCATGGC CGAGCACCTG 
CTGGGTCATG CCTGTGCCCT GGCCGCCTCC CTCTGGCTGG GGGTGCTGCC CACACTCACT 
CTTGGCTTGC TGGTCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA7A ( NM_003612.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003603.1 Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23073
Clone ID Related Accession (Same CDS sequence)	NM_003612.5 , NM_003612.4
Accession Version	NM_003612.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	semaphorin-7A isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA815098.1, AK312257.1, AK001895.1, AC090826.15, BF792612.1 and AI972833.1. On Feb 18, 2016 this sequence version replaced NM_003612.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF069493.1, AF030698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GTGAATATCG GCTCCACAAA GGGGTCCTGT 
CTGGATAAGC GGGACTGCGA GAACTACATC ACTCTCCTGG AGAGGCGGAG TGAGGGGCTG 
CTGGCCTGTG GCACCAACGC CCGGCACCCC AGCTGCTGGA ACCTGGTGAA TGGCACTGTG 
GTGCCACTTG GCGAGATGAG AGGCTACGCC CCCTTCAGCC CGGACGAGAA CTCCCTGGTT 
CTGTTTGAAG GGGACGAGGT GTATTCCACC ATCCGGAAGC AGGAATACAA TGGGAAGATC 
CCTCGGTTCC GCCGCATCCG GGGCGAGAGT GAGCTGTACA CCAGTGATAC TGTCATGCAG 
AACCCACAGT TCATCAAAGC CACCATCGTG CACCAAGACC AGGCTTACGA TGACAAGATC 
TACTACTTCT TCCGAGAGGA CAATCCTGAC AAGAATCCTG AGGCTCCTCT CAATGTGTCC 
CGTGTGGCCC AGTTGTGCAG GGGGGACCAG GGTGGGGAAA GTTCACTGTC AGTCTCCAAG 
TGGAACACTT TTCTGAAAGC CATGCTGGTA TGCAGTGATG CTGCCACCAA CAAGAACTTC 
AACAGGCTGC AAGACGTCTT CCTGCTCCCT GACCCCAGCG GCCAGTGGAG GGACACCAGG 
GTCTATGGTG TTTTCTCCAA CCCCTGGAAC TACTCAGCCG TCTGTGTGTA TTCCCTCGGT 
GACATTGACA AGGTCTTCCG TACCTCCTCA CTCAAGGGCT ACCACTCAAG CCTTCCCAAC 
CCGCGGCCTG GCAAGTGCCT CCCAGACCAG CAGCCGATAC CCACAGAGAC CTTCCAGGTG 
GCTGACCGTC ACCCAGAGGT GGCGCAGAGG GTGGAGCCCA TGGGGCCTCT GAAGACGCCA 
TTGTTCCACT CTAAATACCA CTACCAGAAA GTGGCCGTCC ACCGCATGCA AGCCAGCCAC 
GGGGAGACCT TTCATGTGCT TTACCTAACT ACAGACAGGG GCACTATCCA CAAGGTGGTG 
GAACCGGGGG AGCAGGAGCA CAGCTTCGCC TTCAACATCA TGGAGATCCA GCCCTTCCGC 
CGCGCGGCTG CCATCCAGAC CATGTCGCTG GATGCTGAGC GGAGGAAGCT GTATGTGAGC 
TCCCAGTGGG AGGTGAGCCA GGTGCCCCTG GACCTGTGTG AGGTCTATGG CGGGGGCTGC 
CACGGTTGCC TCATGTCCCG AGACCCCTAC TGCGGCTGGG ACCAAGGCCG CTGCATCTCC 
ATCTACAGCT CCGAACGGTC AGTGCTGCAA TCCATTAATC CAGCCGAGCC ACACAAGGAG 
TGTCCCAACC CCAAACCAGA CAAGGCCCCA CTGCAGAAGG TTTCCCTGGC CCCAAACTCT 
CGCTACTACC TGAGCTGCCC CATGGAATCC CGCCACGCCA CCTACTCATG GCGCCACAAG 
GAGAACGTGG AGCAGAGCTG CGAACCTGGT CACCAGAGCC CCAACTGCAT CCTGTTCATC 
GAGAACCTCA CGGCGCAGCA GTACGGCCAC TACTTCTGCG AGGCCCAGGA GGGCTCCTAC 
TTCCGCGAGG CTCAGCACTG GCAGCTGCTG CCCGAGGACG GCATCATGGC CGAGCACCTG 
CTGGGTCATG CCTGTGCCCT GGCCGCCTCC CTCTGGCTGG GGGTGCTGCC CACACTCACT 
CTTGGCTTGC TGGTCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003603.1
CDS	42..2042
Translation	MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH

Target ORF information:

RefSeq Version	NM_003612.4
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003612.4

ORF Insert Sequence:

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GTGAATATCG GCTCCACAAA GGGGTCCTGT 
CTGGATAAGC GGGACTGCGA GAACTACATC ACTCTCCTGG AGAGGCGGAG TGAGGGGCTG 
CTGGCCTGTG GCACCAACGC CCGGCACCCC AGCTGCTGGA ACCTGGTGAA TGGCACTGTG 
GTGCCACTTG GCGAGATGAG AGGCTACGCC CCCTTCAGCC CGGACGAGAA CTCCCTGGTT 
CTGTTTGAAG GGGACGAGGT GTATTCCACC ATCCGGAAGC AGGAATACAA TGGGAAGATC 
CCTCGGTTCC GCCGCATCCG GGGCGAGAGT GAGCTGTACA CCAGTGATAC TGTCATGCAG 
AACCCACAGT TCATCAAAGC CACCATCGTG CACCAAGACC AGGCTTACGA TGACAAGATC 
TACTACTTCT TCCGAGAGGA CAATCCTGAC AAGAATCCTG AGGCTCCTCT CAATGTGTCC 
CGTGTGGCCC AGTTGTGCAG GGGGGACCAG GGTGGGGAAA GTTCACTGTC AGTCTCCAAG 
TGGAACACTT TTCTGAAAGC CATGCTGGTA TGCAGTGATG CTGCCACCAA CAAGAACTTC 
AACAGGCTGC AAGACGTCTT CCTGCTCCCT GACCCCAGCG GCCAGTGGAG GGACACCAGG 
GTCTATGGTG TTTTCTCCAA CCCCTGGAAC TACTCAGCCG TCTGTGTGTA TTCCCTCGGT 
GACATTGACA AGGTCTTCCG TACCTCCTCA CTCAAGGGCT ACCACTCAAG CCTTCCCAAC 
CCGCGGCCTG GCAAGTGCCT CCCAGACCAG CAGCCGATAC CCACAGAGAC CTTCCAGGTG 
GCTGACCGTC ACCCAGAGGT GGCGCAGAGG GTGGAGCCCA TGGGGCCTCT GAAGACGCCA 
TTGTTCCACT CTAAATACCA CTACCAGAAA GTGGCCGTCC ACCGCATGCA AGCCAGCCAC 
GGGGAGACCT TTCATGTGCT TTACCTAACT ACAGACAGGG GCACTATCCA CAAGGTGGTG 
GAACCGGGGG AGCAGGAGCA CAGCTTCGCC TTCAACATCA TGGAGATCCA GCCCTTCCGC 
CGCGCGGCTG CCATCCAGAC CATGTCGCTG GATGCTGAGC GGAGGAAGCT GTATGTGAGC 
TCCCAGTGGG AGGTGAGCCA GGTGCCCCTG GACCTGTGTG AGGTCTATGG CGGGGGCTGC 
CACGGTTGCC TCATGTCCCG AGACCCCTAC TGCGGCTGGG ACCAAGGCCG CTGCATCTCC 
ATCTACAGCT CCGAACGGTC AGTGCTGCAA TCCATTAATC CAGCCGAGCC ACACAAGGAG 
TGTCCCAACC CCAAACCAGA CAAGGCCCCA CTGCAGAAGG TTTCCCTGGC CCCAAACTCT 
CGCTACTACC TGAGCTGCCC CATGGAATCC CGCCACGCCA CCTACTCATG GCGCCACAAG 
GAGAACGTGG AGCAGAGCTG CGAACCTGGT CACCAGAGCC CCAACTGCAT CCTGTTCATC 
GAGAACCTCA CGGCGCAGCA GTACGGCCAC TACTTCTGCG AGGCCCAGGA GGGCTCCTAC 
TTCCGCGAGG CTCAGCACTG GCAGCTGCTG CCCGAGGACG GCATCATGGC CGAGCACCTG 
CTGGGTCATG CCTGTGCCCT GGCCGCCTCC CTCTGGCTGG GGGTGCTGCC CACACTCACT 
CTTGGCTTGC TGGTCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA7A ( NM_001146030.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139502.1 Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 3, mRNA.

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CloneID	OHu12063
Clone ID Related Accession (Same CDS sequence)	NM_001146030.2
Accession Version	NM_001146030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	semaphorin-7A isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA232032.1, DA136330.1, AK293280.1, AK001895.1, AC090826.15, BF792612.1 and AI972833.1. On Feb 18, 2016 this sequence version replaced NM_001146030.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter N-terminus and lacks a predicted signal peptide compared to isoform 1. Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGGCT ACGCCCCCTT CAGCCCGGAC GAGAACTCCC TGGTTCTGTT TGAAGGGGAC 
GAGGTGTATT CCACCATCCG GAAGCAGGAA TACAATGGGA AGATCCCTCG GTTCCGCCGC 
ATCCGGGGCG AGAGTGAGCT GTACACCAGT GATACTGTCA TGCAGAACCC ACAGTTCATC 
AAAGCCACCA TCGTGCACCA AGACCAGGCT TACGATGACA AGATCTACTA CTTCTTCCGA 
GAGGACAATC CTGACAAGAA TCCTGAGGCT CCTCTCAATG TGTCCCGTGT GGCCCAGTTG 
TGCAGGGGGG ACCAGGGTGG GGAAAGTTCA CTGTCAGTCT CCAAGTGGAA CACTTTTCTG 
AAAGCCATGC TGGTATGCAG TGATGCTGCC ACCAACAAGA ACTTCAACAG GCTGCAAGAC 
GTCTTCCTGC TCCCTGACCC CAGCGGCCAG TGGAGGGACA CCAGGGTCTA TGGTGTTTTC 
TCCAACCCCT GGAACTACTC AGCCGTCTGT GTGTATTCCC TCGGTGACAT TGACAAGGTC 
TTCCGTACCT CCTCACTCAA GGGCTACCAC TCAAGCCTTC CCAACCCGCG GCCTGGCAAG 
TGCCTCCCAG ACCAGCAGCC GATACCCACA GAGACCTTCC AGGTGGCTGA CCGTCACCCA 
GAGGTGGCGC AGAGGGTGGA GCCCATGGGG CCTCTGAAGA CGCCATTGTT CCACTCTAAA 
TACCACTACC AGAAAGTGGC CGTCCACCGC ATGCAAGCCA GCCACGGGGA GACCTTTCAT 
GTGCTTTACC TAACTACAGA CAGGGGCACT ATCCACAAGG TGGTGGAACC GGGGGAGCAG 
GAGCACAGCT TCGCCTTCAA CATCATGGAG ATCCAGCCCT TCCGCCGCGC GGCTGCCATC 
CAGACCATGT CGCTGGATGC TGAGCGGAGG AAGCTGTATG TGAGCTCCCA GTGGGAGGTG 
AGCCAGGTGC CCCTGGACCT GTGTGAGGTC TATGGCGGGG GCTGCCACGG TTGCCTCATG 
TCCCGAGACC CCTACTGCGG CTGGGACCAA GGCCGCTGCA TCTCCATCTA CAGCTCCGAA 
CGGTCAGTGC TGCAATCCAT TAATCCAGCC GAGCCACACA AGGAGTGTCC CAACCCCAAA 
CCAGACAAGG CCCCACTGCA GAAGGTTTCC CTGGCCCCAA ACTCTCGCTA CTACCTGAGC 
TGCCCCATGG AATCCCGCCA CGCCACCTAC TCATGGCGCC ACAAGGAGAA CGTGGAGCAG 
AGCTGCGAAC CTGGTCACCA GAGCCCCAAC TGCATCCTGT TCATCGAGAA CCTCACGGCG 
CAGCAGTACG GCCACTACTT CTGCGAGGCC CAGGAGGGCT CCTACTTCCG CGAGGCTCAG 
CACTGGCAGC TGCTGCCCGA GGACGGCATC ATGGCCGAGC ACCTGCTGGG TCATGCCTGT 
GCCCTGGCCG CCTCCCTCTG GCTGGGGGTG CTGCCCACAC TCACTCTTGG CTTGCTGGTC 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139502.1
CDS	433..1938
Translation	MRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH

Target ORF information:

RefSeq Version	NM_001146030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146030.2

ORF Insert Sequence:

ATGAGAGGCT ACGCCCCCTT CAGCCCGGAC GAGAACTCCC TGGTTCTGTT TGAAGGGGAC 
GAGGTGTATT CCACCATCCG GAAGCAGGAA TACAATGGGA AGATCCCTCG GTTCCGCCGC 
ATCCGGGGCG AGAGTGAGCT GTACACCAGT GATACTGTCA TGCAGAACCC ACAGTTCATC 
AAAGCCACCA TCGTGCACCA AGACCAGGCT TACGATGACA AGATCTACTA CTTCTTCCGA 
GAGGACAATC CTGACAAGAA TCCTGAGGCT CCTCTCAATG TGTCCCGTGT GGCCCAGTTG 
TGCAGGGGGG ACCAGGGTGG GGAAAGTTCA CTGTCAGTCT CCAAGTGGAA CACTTTTCTG 
AAAGCCATGC TGGTATGCAG TGATGCTGCC ACCAACAAGA ACTTCAACAG GCTGCAAGAC 
GTCTTCCTGC TCCCTGACCC CAGCGGCCAG TGGAGGGACA CCAGGGTCTA TGGTGTTTTC 
TCCAACCCCT GGAACTACTC AGCCGTCTGT GTGTATTCCC TCGGTGACAT TGACAAGGTC 
TTCCGTACCT CCTCACTCAA GGGCTACCAC TCAAGCCTTC CCAACCCGCG GCCTGGCAAG 
TGCCTCCCAG ACCAGCAGCC GATACCCACA GAGACCTTCC AGGTGGCTGA CCGTCACCCA 
GAGGTGGCGC AGAGGGTGGA GCCCATGGGG CCTCTGAAGA CGCCATTGTT CCACTCTAAA 
TACCACTACC AGAAAGTGGC CGTCCACCGC ATGCAAGCCA GCCACGGGGA GACCTTTCAT 
GTGCTTTACC TAACTACAGA CAGGGGCACT ATCCACAAGG TGGTGGAACC GGGGGAGCAG 
GAGCACAGCT TCGCCTTCAA CATCATGGAG ATCCAGCCCT TCCGCCGCGC GGCTGCCATC 
CAGACCATGT CGCTGGATGC TGAGCGGAGG AAGCTGTATG TGAGCTCCCA GTGGGAGGTG 
AGCCAGGTGC CCCTGGACCT GTGTGAGGTC TATGGCGGGG GCTGCCACGG TTGCCTCATG 
TCCCGAGACC CCTACTGCGG CTGGGACCAA GGCCGCTGCA TCTCCATCTA CAGCTCCGAA 
CGGTCAGTGC TGCAATCCAT TAATCCAGCC GAGCCACACA AGGAGTGTCC CAACCCCAAA 
CCAGACAAGG CCCCACTGCA GAAGGTTTCC CTGGCCCCAA ACTCTCGCTA CTACCTGAGC 
TGCCCCATGG AATCCCGCCA CGCCACCTAC TCATGGCGCC ACAAGGAGAA CGTGGAGCAG 
AGCTGCGAAC CTGGTCACCA GAGCCCCAAC TGCATCCTGT TCATCGAGAA CCTCACGGCG 
CAGCAGTACG GCCACTACTT CTGCGAGGCC CAGGAGGGCT CCTACTTCCG CGAGGCTCAG 
CACTGGCAGC TGCTGCCCGA GGACGGCATC ATGGCCGAGC ACCTGCTGGG TCATGCCTGT 
GCCCTGGCCG CCTCCCTCTG GCTGGGGGTG CTGCCCACAC TCACTCTTGG CTTGCTGGTC 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA7A ( NM_001146029.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139501.1 Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.

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CloneID	OHu10551
Clone ID Related Accession (Same CDS sequence)	NM_001146029.3 , NM_001146029.2
Accession Version	NM_001146029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	semaphorin-7A isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA815098.1, AK293280.1, AK001895.1, AC090826.15, BF792612.1 and AI972833.1. On Jun 1, 2019 this sequence version replaced NM_001146029.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. The encoded isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GACTGCGAGA ACTACATCAC TCTCCTGGAG 
AGGCGGAGTG AGGGGCTGCT GGCCTGTGGC ACCAACGCCC GGCACCCCAG CTGCTGGAAC 
CTGGTGAATG GCACTGTGGT GCCACTTGGC GAGATGAGAG GCTACGCCCC CTTCAGCCCG 
GACGAGAACT CCCTGGTTCT GTTTGAAGGG GACGAGGTGT ATTCCACCAT CCGGAAGCAG 
GAATACAATG GGAAGATCCC TCGGTTCCGC CGCATCCGGG GCGAGAGTGA GCTGTACACC 
AGTGATACTG TCATGCAGAA CCCACAGTTC ATCAAAGCCA CCATCGTGCA CCAAGACCAG 
GCTTACGATG ACAAGATCTA CTACTTCTTC CGAGAGGACA ATCCTGACAA GAATCCTGAG 
GCTCCTCTCA ATGTGTCCCG TGTGGCCCAG TTGTGCAGGG GGGACCAGGG TGGGGAAAGT 
TCACTGTCAG TCTCCAAGTG GAACACTTTT CTGAAAGCCA TGCTGGTATG CAGTGATGCT 
GCCACCAACA AGAACTTCAA CAGGCTGCAA GACGTCTTCC TGCTCCCTGA CCCCAGCGGC 
CAGTGGAGGG ACACCAGGGT CTATGGTGTT TTCTCCAACC CCTGGAACTA CTCAGCCGTC 
TGTGTGTATT CCCTCGGTGA CATTGACAAG GTCTTCCGTA CCTCCTCACT CAAGGGCTAC 
CACTCAAGCC TTCCCAACCC GCGGCCTGGC AAGTGCCTCC CAGACCAGCA GCCGATACCC 
ACAGAGACCT TCCAGGTGGC TGACCGTCAC CCAGAGGTGG CGCAGAGGGT GGAGCCCATG 
GGGCCTCTGA AGACGCCATT GTTCCACTCT AAATACCACT ACCAGAAAGT GGCCGTCCAC 
CGCATGCAAG CCAGCCACGG GGAGACCTTT CATGTGCTTT ACCTAACTAC AGACAGGGGC 
ACTATCCACA AGGTGGTGGA ACCGGGGGAG CAGGAGCACA GCTTCGCCTT CAACATCATG 
GAGATCCAGC CCTTCCGCCG CGCGGCTGCC ATCCAGACCA TGTCGCTGGA TGCTGAGCGG 
AGGAAGCTGT ATGTGAGCTC CCAGTGGGAG GTGAGCCAGG TGCCCCTGGA CCTGTGTGAG 
GTCTATGGCG GGGGCTGCCA CGGTTGCCTC ATGTCCCGAG ACCCCTACTG CGGCTGGGAC 
CAAGGCCGCT GCATCTCCAT CTACAGCTCC GAACGGTCAG TGCTGCAATC CATTAATCCA 
GCCGAGCCAC ACAAGGAGTG TCCCAACCCC AAACCAGACA AGGCCCCACT GCAGAAGGTT 
TCCCTGGCCC CAAACTCTCG CTACTACCTG AGCTGCCCCA TGGAATCCCG CCACGCCACC 
TACTCATGGC GCCACAAGGA GAACGTGGAG CAGAGCTGCG AACCTGGTCA CCAGAGCCCC 
AACTGCATCC TGTTCATCGA GAACCTCACG GCGCAGCAGT ACGGCCACTA CTTCTGCGAG 
GCCCAGGAGG GCTCCTACTT CCGCGAGGCT CAGCACTGGC AGCTGCTGCC CGAGGACGGC 
ATCATGGCCG AGCACCTGCT GGGTCATGCC TGTGCCCTGG CCGCCTCCCT CTGGCTGGGG 
GTGCTGCCCA CACTCACTCT TGGCTTGCTG GTCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139501.1
CDS	41..1999
Translation	MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH

Target ORF information:

RefSeq Version	NM_001146029.3
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146029.3

ORF Insert Sequence:

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GACTGCGAGA ACTACATCAC TCTCCTGGAG 
AGGCGGAGTG AGGGGCTGCT GGCCTGTGGC ACCAACGCCC GGCACCCCAG CTGCTGGAAC 
CTGGTGAATG GCACTGTGGT GCCACTTGGC GAGATGAGAG GCTACGCCCC CTTCAGCCCG 
GACGAGAACT CCCTGGTTCT GTTTGAAGGG GACGAGGTGT ATTCCACCAT CCGGAAGCAG 
GAATACAATG GGAAGATCCC TCGGTTCCGC CGCATCCGGG GCGAGAGTGA GCTGTACACC 
AGTGATACTG TCATGCAGAA CCCACAGTTC ATCAAAGCCA CCATCGTGCA CCAAGACCAG 
GCTTACGATG ACAAGATCTA CTACTTCTTC CGAGAGGACA ATCCTGACAA GAATCCTGAG 
GCTCCTCTCA ATGTGTCCCG TGTGGCCCAG TTGTGCAGGG GGGACCAGGG TGGGGAAAGT 
TCACTGTCAG TCTCCAAGTG GAACACTTTT CTGAAAGCCA TGCTGGTATG CAGTGATGCT 
GCCACCAACA AGAACTTCAA CAGGCTGCAA GACGTCTTCC TGCTCCCTGA CCCCAGCGGC 
CAGTGGAGGG ACACCAGGGT CTATGGTGTT TTCTCCAACC CCTGGAACTA CTCAGCCGTC 
TGTGTGTATT CCCTCGGTGA CATTGACAAG GTCTTCCGTA CCTCCTCACT CAAGGGCTAC 
CACTCAAGCC TTCCCAACCC GCGGCCTGGC AAGTGCCTCC CAGACCAGCA GCCGATACCC 
ACAGAGACCT TCCAGGTGGC TGACCGTCAC CCAGAGGTGG CGCAGAGGGT GGAGCCCATG 
GGGCCTCTGA AGACGCCATT GTTCCACTCT AAATACCACT ACCAGAAAGT GGCCGTCCAC 
CGCATGCAAG CCAGCCACGG GGAGACCTTT CATGTGCTTT ACCTAACTAC AGACAGGGGC 
ACTATCCACA AGGTGGTGGA ACCGGGGGAG CAGGAGCACA GCTTCGCCTT CAACATCATG 
GAGATCCAGC CCTTCCGCCG CGCGGCTGCC ATCCAGACCA TGTCGCTGGA TGCTGAGCGG 
AGGAAGCTGT ATGTGAGCTC CCAGTGGGAG GTGAGCCAGG TGCCCCTGGA CCTGTGTGAG 
GTCTATGGCG GGGGCTGCCA CGGTTGCCTC ATGTCCCGAG ACCCCTACTG CGGCTGGGAC 
CAAGGCCGCT GCATCTCCAT CTACAGCTCC GAACGGTCAG TGCTGCAATC CATTAATCCA 
GCCGAGCCAC ACAAGGAGTG TCCCAACCCC AAACCAGACA AGGCCCCACT GCAGAAGGTT 
TCCCTGGCCC CAAACTCTCG CTACTACCTG AGCTGCCCCA TGGAATCCCG CCACGCCACC 
TACTCATGGC GCCACAAGGA GAACGTGGAG CAGAGCTGCG AACCTGGTCA CCAGAGCCCC 
AACTGCATCC TGTTCATCGA GAACCTCACG GCGCAGCAGT ACGGCCACTA CTTCTGCGAG 
GCCCAGGAGG GCTCCTACTT CCGCGAGGCT CAGCACTGGC AGCTGCTGCC CGAGGACGGC 
ATCATGGCCG AGCACCTGCT GGGTCATGCC TGTGCCCTGG CCGCCTCCCT CTGGCTGGGG 
GTGCTGCCCA CACTCACTCT TGGCTTGCTG GTCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA7A ( NM_001146029.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139501.1 Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10551
Clone ID Related Accession (Same CDS sequence)	NM_001146029.3 , NM_001146029.2
Accession Version	NM_001146029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	semaphorin-7A isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA815098.1, AK293280.1, AK001895.1, AC090826.15, BF792612.1 and AI972833.1. On Feb 18, 2016 this sequence version replaced NM_001146029.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. The encoded isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record represents the SEMA7A*001.1.1 allele. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GACTGCGAGA ACTACATCAC TCTCCTGGAG 
AGGCGGAGTG AGGGGCTGCT GGCCTGTGGC ACCAACGCCC GGCACCCCAG CTGCTGGAAC 
CTGGTGAATG GCACTGTGGT GCCACTTGGC GAGATGAGAG GCTACGCCCC CTTCAGCCCG 
GACGAGAACT CCCTGGTTCT GTTTGAAGGG GACGAGGTGT ATTCCACCAT CCGGAAGCAG 
GAATACAATG GGAAGATCCC TCGGTTCCGC CGCATCCGGG GCGAGAGTGA GCTGTACACC 
AGTGATACTG TCATGCAGAA CCCACAGTTC ATCAAAGCCA CCATCGTGCA CCAAGACCAG 
GCTTACGATG ACAAGATCTA CTACTTCTTC CGAGAGGACA ATCCTGACAA GAATCCTGAG 
GCTCCTCTCA ATGTGTCCCG TGTGGCCCAG TTGTGCAGGG GGGACCAGGG TGGGGAAAGT 
TCACTGTCAG TCTCCAAGTG GAACACTTTT CTGAAAGCCA TGCTGGTATG CAGTGATGCT 
GCCACCAACA AGAACTTCAA CAGGCTGCAA GACGTCTTCC TGCTCCCTGA CCCCAGCGGC 
CAGTGGAGGG ACACCAGGGT CTATGGTGTT TTCTCCAACC CCTGGAACTA CTCAGCCGTC 
TGTGTGTATT CCCTCGGTGA CATTGACAAG GTCTTCCGTA CCTCCTCACT CAAGGGCTAC 
CACTCAAGCC TTCCCAACCC GCGGCCTGGC AAGTGCCTCC CAGACCAGCA GCCGATACCC 
ACAGAGACCT TCCAGGTGGC TGACCGTCAC CCAGAGGTGG CGCAGAGGGT GGAGCCCATG 
GGGCCTCTGA AGACGCCATT GTTCCACTCT AAATACCACT ACCAGAAAGT GGCCGTCCAC 
CGCATGCAAG CCAGCCACGG GGAGACCTTT CATGTGCTTT ACCTAACTAC AGACAGGGGC 
ACTATCCACA AGGTGGTGGA ACCGGGGGAG CAGGAGCACA GCTTCGCCTT CAACATCATG 
GAGATCCAGC CCTTCCGCCG CGCGGCTGCC ATCCAGACCA TGTCGCTGGA TGCTGAGCGG 
AGGAAGCTGT ATGTGAGCTC CCAGTGGGAG GTGAGCCAGG TGCCCCTGGA CCTGTGTGAG 
GTCTATGGCG GGGGCTGCCA CGGTTGCCTC ATGTCCCGAG ACCCCTACTG CGGCTGGGAC 
CAAGGCCGCT GCATCTCCAT CTACAGCTCC GAACGGTCAG TGCTGCAATC CATTAATCCA 
GCCGAGCCAC ACAAGGAGTG TCCCAACCCC AAACCAGACA AGGCCCCACT GCAGAAGGTT 
TCCCTGGCCC CAAACTCTCG CTACTACCTG AGCTGCCCCA TGGAATCCCG CCACGCCACC 
TACTCATGGC GCCACAAGGA GAACGTGGAG CAGAGCTGCG AACCTGGTCA CCAGAGCCCC 
AACTGCATCC TGTTCATCGA GAACCTCACG GCGCAGCAGT ACGGCCACTA CTTCTGCGAG 
GCCCAGGAGG GCTCCTACTT CCGCGAGGCT CAGCACTGGC AGCTGCTGCC CGAGGACGGC 
ATCATGGCCG AGCACCTGCT GGGTCATGCC TGTGCCCTGG CCGCCTCCCT CTGGCTGGGG 
GTGCTGCCCA CACTCACTCT TGGCTTGCTG GTCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139501.1
CDS	42..2000
Translation	MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH

Target ORF information:

RefSeq Version	NM_001146029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 7A (John Milton Hagen blood group) (SEMA7A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146029.2

ORF Insert Sequence:

ATGACGCCTC CTCCGCCCGG ACGTGCCGCC CCCAGCGCAC CGCGCGCCCG CGTCCCTGGC 
CCGCCGGCTC GGTTGGGGCT TCCGCTGCGG CTGCGGCTGC TGCTGCTGCT CTGGGCGGCC 
GCCGCCTCCG CCCAGGGCCA CCTAAGGAGC GGACCCCGCA TCTTCGCCGT CTGGAAAGGC 
CATGTAGGGC AGGACCGGGT GGACTTTGGC CAGACTGAGC CGCACACGGT GCTTTTCCAC 
GAGCCAGGCA GCTCCTCTGT GTGGGTGGGA GGACGTGGCA AGGTCTACCT CTTTGACTTC 
CCCGAGGGCA AGAACGCATC TGTGCGCACG GACTGCGAGA ACTACATCAC TCTCCTGGAG 
AGGCGGAGTG AGGGGCTGCT GGCCTGTGGC ACCAACGCCC GGCACCCCAG CTGCTGGAAC 
CTGGTGAATG GCACTGTGGT GCCACTTGGC GAGATGAGAG GCTACGCCCC CTTCAGCCCG 
GACGAGAACT CCCTGGTTCT GTTTGAAGGG GACGAGGTGT ATTCCACCAT CCGGAAGCAG 
GAATACAATG GGAAGATCCC TCGGTTCCGC CGCATCCGGG GCGAGAGTGA GCTGTACACC 
AGTGATACTG TCATGCAGAA CCCACAGTTC ATCAAAGCCA CCATCGTGCA CCAAGACCAG 
GCTTACGATG ACAAGATCTA CTACTTCTTC CGAGAGGACA ATCCTGACAA GAATCCTGAG 
GCTCCTCTCA ATGTGTCCCG TGTGGCCCAG TTGTGCAGGG GGGACCAGGG TGGGGAAAGT 
TCACTGTCAG TCTCCAAGTG GAACACTTTT CTGAAAGCCA TGCTGGTATG CAGTGATGCT 
GCCACCAACA AGAACTTCAA CAGGCTGCAA GACGTCTTCC TGCTCCCTGA CCCCAGCGGC 
CAGTGGAGGG ACACCAGGGT CTATGGTGTT TTCTCCAACC CCTGGAACTA CTCAGCCGTC 
TGTGTGTATT CCCTCGGTGA CATTGACAAG GTCTTCCGTA CCTCCTCACT CAAGGGCTAC 
CACTCAAGCC TTCCCAACCC GCGGCCTGGC AAGTGCCTCC CAGACCAGCA GCCGATACCC 
ACAGAGACCT TCCAGGTGGC TGACCGTCAC CCAGAGGTGG CGCAGAGGGT GGAGCCCATG 
GGGCCTCTGA AGACGCCATT GTTCCACTCT AAATACCACT ACCAGAAAGT GGCCGTCCAC 
CGCATGCAAG CCAGCCACGG GGAGACCTTT CATGTGCTTT ACCTAACTAC AGACAGGGGC 
ACTATCCACA AGGTGGTGGA ACCGGGGGAG CAGGAGCACA GCTTCGCCTT CAACATCATG 
GAGATCCAGC CCTTCCGCCG CGCGGCTGCC ATCCAGACCA TGTCGCTGGA TGCTGAGCGG 
AGGAAGCTGT ATGTGAGCTC CCAGTGGGAG GTGAGCCAGG TGCCCCTGGA CCTGTGTGAG 
GTCTATGGCG GGGGCTGCCA CGGTTGCCTC ATGTCCCGAG ACCCCTACTG CGGCTGGGAC 
CAAGGCCGCT GCATCTCCAT CTACAGCTCC GAACGGTCAG TGCTGCAATC CATTAATCCA 
GCCGAGCCAC ACAAGGAGTG TCCCAACCCC AAACCAGACA AGGCCCCACT GCAGAAGGTT 
TCCCTGGCCC CAAACTCTCG CTACTACCTG AGCTGCCCCA TGGAATCCCG CCACGCCACC 
TACTCATGGC GCCACAAGGA GAACGTGGAG CAGAGCTGCG AACCTGGTCA CCAGAGCCCC 
AACTGCATCC TGTTCATCGA GAACCTCACG GCGCAGCAGT ACGGCCACTA CTTCTGCGAG 
GCCCAGGAGG GCTCCTACTT CCGCGAGGCT CAGCACTGGC AGCTGCTGCC CGAGGACGGC 
ATCATGGCCG AGCACCTGCT GGGTCATGCC TGTGCCCTGG CCGCCTCCCT CTGGCTGGGG 
GTGCTGCCCA CACTCACTCT TGGCTTGCTG GTCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vascular Semaphorin 7A Upregulation by Disturbed Flow Promotes Atherosclerosis Through Endothelial ?1 Integrin.
Arteriosclerosis, thrombosis, and vascular biology(2017 Dec)
Hu S,Liu Y,You T,Heath J,Xu L,Zheng X,Wang A,Wang Y,Li F,Yang F,Cao Y,Zhang H,van Gils JM,van Zonneveld AJ,Jo H,Wu Q,Zhang Y,Tang C,Zhu L

Identification and Evaluation of New Immunoregulatory Genes in Mesenchymal Stromal Cells of Different Origins: Comparison of Normal and Inflammatory Conditions.
Medical science monitor basic research2387-96(2017 Mar)
Fayyad-Kazan M,Najar M,Fayyad-Kazan H,Raicevic G,Lagneaux L

Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression.
The Journal of investigative dermatology129(4)954-63(2009 Apr)
Scott GA,McClelland LA,Fricke AF,Fender A

Semaphorin 7a promotes spreading and dendricity in human melanocytes through beta1-integrins.
The Journal of investigative dermatology128(1)151-61(2008 Jan)
Scott GA,McClelland LA,Fricke AF

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SEMA7A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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