NT5C1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NT5C1A
Entrez Gene ID	84618
Full Name	5'-nucleotidase, cytosolic IA
Synonyms	CN-I,CN-IA,CN1,CN1A,CNI
General protein information	Preferred Names 5'-nucleotidase, cytosolic IA Names cytosolic 5'-nucleotidase 1A AMP-specific 5'-NT cytosolic 5' nucleotidase, type 1A cytosolic 5'-nucleotidase IA
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.2
Summary	Cytosolic nucleotidases, such as NT5C1A, dephosphorylate nucleoside monophosphates (Hunsucker et al., 2001 [PubMed 11133996]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610525

mRNA and Protein(s)

mRNA	Protein	Name
NM_032526.2	NP_115915.1	cytosolic 5'-nucleotidase 1A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NT5C1A	NP_001192475.1
Rattus norvegicus (Norway rat)	Nt5c1a	NP_001101446.1
Gallus gallus (chicken)	NT5C1A	XP_417822.3
Mus musculus (house mouse)	Nt5c1a	NP_001078971.1
Danio rerio (zebrafish)	LOC799449	XP_005158936.1
Pan troglodytes (chimpanzee)	NT5C1A	XP_524674.2
	NT5C1A	XP_001113402.1
Canis lupus familiaris (dog)	NT5C1A	XP_539580.3
Danio rerio (zebrafish)	nt5c1a	NP_001122185.1
Homo sapiens (human)	NT5C1A	NP_115915.1
Xenopus tropicalis (tropical clawed frog)	LOC100495335	XP_002941700.1

Gene Ontology
Bibliography

Related articles in PubMed

Pharmacogenetic characterization of naturally occurring germline NT5C1A variants to chemotherapeutic nucleoside analogs.
Saliba J, Zabriskie R, Ghosh R, Powell BC, Hicks S, Kimmel M, Meng Q, Ritter DI, Wheeler DA, Gibbs RA, Tsai FT, Plon SE
Pharmacogenetics and genomics26(6)271-9(2016 06)

Seropositivity for NT5c1A antibody in sporadic inclusion body myositis predicts more severe motor, bulbar and respiratory involvement.
Goyal NA, Cash TM, Alam U, Enam S, Tierney P, Araujo N, Mozaffar FH, Pestronk A, Mozaffar T
Journal of neurology, neurosurgery, and psychiatry87(4)373-8(2016 Apr)

Cytosolic 5'-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases.
Lloyd TE, Christopher-Stine L, Pinal-Fernandez I, Tiniakou E, Petri M, Baer A, Danoff SK, Pak K, Casciola-Rosen LA, Mammen AL
Arthritis care & research68(1)66-71(2016 Jan)

Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
Kulkarni SS, Karlsson HK, Szekeres F, Chibalin AV, Krook A, Zierath JR
The Journal of biological chemistry286(40)34567-74(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Individual missense changes in NT5C1A showed considerable variation in response to the different nucleoside analogs tested.
Title: Pharmacogenetic characterization of naturally occurring germline NT5C1A variants to chemotherapeutic nucleoside analogs.

seropositivity to the NT5c1A antibody is associated with greater motor and functional disability in sporadic inclusion body myositis
Title: Seropositivity for NT5c1A antibody in sporadic inclusion body myositis predicts more severe motor, bulbar and respiratory involvement.

Anti-NT5C1A is a common target of circulating autoantibodies in autoimmune diseases.
Title: Cytosolic 5'-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases.

NT5C1A suppression promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle
Title: Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.

There was no correlation between expression of cN-I and fiber type.
Title: Expression of cytosolic 5' nucleotidase does not correlate with expression of oxidative metabolism marker: myoglobine in human skeletal muscles.

The following NT5C1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NT5C1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu73608	NM_032526.2 Latest version!	Homo sapiens 5'-nucleotidase, cytosolic IA (NT5C1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$237.30 $339.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NT5C1A ( NM_032526.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115915.1 Homo sapiens 5'-nucleotidase, cytosolic IA (NT5C1A), mRNA.

Price & Availability↑

CloneID	OHu73608
Clone ID Related Accession (Same CDS sequence)	NM_032526.2
Accession Version	NM_032526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	cytosolic 5'-nucleotidase 1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC103879.1. On Dec 16, 2014 this sequence version replaced NM_032526.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC103879.1, AF331801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAACCTG GGCAGCCCCG GGAGCCCCAG GAGCCCCGCG AGCCCGGGCC AGGAGCGGAG 
ACCGCTGCGG CCCCGGTCTG GGAGGAAGCC AAGATTTTCT ACGACAACCT CGCGCCCAAG 
AAGAAACCCA AATCGCCCAA GCCTCAGAAT GCAGTCACCA TCGCTGTGTC CTCCCGAGCC 
TTGTTTCGCA TGGACGAGGA GCAGCAGATC TACACGGAGC AGGGCGTGGA GGAGTACGTG 
CGCTACCAGC TGGAACATGA GAACGAACCC TTCAGTCCCG GGCCAGCCTT CCCTTTTGTG 
AAGGCTCTGG AGGCCGTGAA CAGGCGGCTG CGGGAGCTGT ACCCTGATAG TGAGGACGTC 
TTCGACATCG TCCTCATGAC TAACAACCAT GCTCAAGTGG GTGTCCGCCT CATCAACAGT 
ATCAACCACT ATGACCTGTT CATCGAGAGG TTCTGCATGA CAGGTGGGAA CAGCCCGATC 
TGCTACCTCA AGGCCTATCA CACCAACCTC TACTTGTCAG CCGATGCGGA AAAAGTGCGA 
GAAGCCATTG ATGAGGGGAT CGCAGCTGCC ACCATCTTCA GCCCCAGCAG GGATGTGGTT 
GTGTCCCAGA GTCAGCTGCG CGTGGCCTTC GATGGGGACG CCGTGCTCTT CTCGGACGAG 
TCGGAGCGCA TCGTCAAGGC CCACGGGCTG GACCGATTCT TCGAGCATGA GAAGGCCCAC 
GAGAACAAAC CTCTGGCTCA GGGCCCCTTA AAGGGCTTTC TGGAGGCACT GGGTAGGTTG 
CAGAAGAAGT TCTACTCCAA AGGCCTGCGG CTGGAGTGCC CAATTCGTAC CTACTTGGTG 
ACAGCACGCA GTGCAGCCAG TTCCGGGGCC CGGGCTCTCA AGACCCTGCG CAGCTGGGGC 
CTGGAGACAG ATGAAGCCTT GTTCCTTGCT GGAGCGCCCA AGGGCCCTCT CCTTGAGAAG 
ATCCGCCCAC ACATCTTCTT TGATGACCAG ATGTTCCATG TGGCTGGGGC TCAGGAGATG 
GGCACTGTGG CCGCCCATGT GCCTTATGGT GTGGCACAGA CACCCCGGCG GACTGCACCT 
GCAAAGCAGG CCCCATCTGC ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115915.1
CDS	1..1107
Translation	MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQNAVTIAVSSRALFRMDEEQQIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQTPRRTAPAKQAPSAQ

Target ORF information:

RefSeq Version	NM_032526.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-nucleotidase, cytosolic IA (NT5C1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032526.2

ORF Insert Sequence:

ATGGAACCTG GGCAGCCCCG GGAGCCCCAG GAGCCCCGCG AGCCCGGGCC AGGAGCGGAG 
ACCGCTGCGG CCCCGGTCTG GGAGGAAGCC AAGATTTTCT ACGACAACCT CGCGCCCAAG 
AAGAAACCCA AATCGCCCAA GCCTCAGAAT GCAGTCACCA TCGCTGTGTC CTCCCGAGCC 
TTGTTTCGCA TGGACGAGGA GCAGCAGATC TACACGGAGC AGGGCGTGGA GGAGTACGTG 
CGCTACCAGC TGGAACATGA GAACGAACCC TTCAGTCCCG GGCCAGCCTT CCCTTTTGTG 
AAGGCTCTGG AGGCCGTGAA CAGGCGGCTG CGGGAGCTGT ACCCTGATAG TGAGGACGTC 
TTCGACATCG TCCTCATGAC TAACAACCAT GCTCAAGTGG GTGTCCGCCT CATCAACAGT 
ATCAACCACT ATGACCTGTT CATCGAGAGG TTCTGCATGA CAGGTGGGAA CAGCCCGATC 
TGCTACCTCA AGGCCTATCA CACCAACCTC TACTTGTCAG CCGATGCGGA AAAAGTGCGA 
GAAGCCATTG ATGAGGGGAT CGCAGCTGCC ACCATCTTCA GCCCCAGCAG GGATGTGGTT 
GTGTCCCAGA GTCAGCTGCG CGTGGCCTTC GATGGGGACG CCGTGCTCTT CTCGGACGAG 
TCGGAGCGCA TCGTCAAGGC CCACGGGCTG GACCGATTCT TCGAGCATGA GAAGGCCCAC 
GAGAACAAAC CTCTGGCTCA GGGCCCCTTA AAGGGCTTTC TGGAGGCACT GGGTAGGTTG 
CAGAAGAAGT TCTACTCCAA AGGCCTGCGG CTGGAGTGCC CAATTCGTAC CTACTTGGTG 
ACAGCACGCA GTGCAGCCAG TTCCGGGGCC CGGGCTCTCA AGACCCTGCG CAGCTGGGGC 
CTGGAGACAG ATGAAGCCTT GTTCCTTGCT GGAGCGCCCA AGGGCCCTCT CCTTGAGAAG 
ATCCGCCCAC ACATCTTCTT TGATGACCAG ATGTTCCATG TGGCTGGGGC TCAGGAGATG 
GGCACTGTGG CCGCCCATGT GCCTTATGGT GTGGCACAGA CACCCCGGCG GACTGCACCT 
GCAAAGCAGG CCCCATCTGC ACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pharmacogenetic characterization of naturally occurring germline NT5C1A variants to chemotherapeutic nucleoside analogs.
Pharmacogenetics and genomics26(6)271-9(2016 06)
Saliba J,Zabriskie R,Ghosh R,Powell BC,Hicks S,Kimmel M,Meng Q,Ritter DI,Wheeler DA,Gibbs RA,Tsai FT,Plon SE

Seropositivity for NT5c1A antibody in sporadic inclusion body myositis predicts more severe motor, bulbar and respiratory involvement.
Journal of neurology, neurosurgery, and psychiatry87(4)373-8(2016 Apr)
Goyal NA,Cash TM,Alam U,Enam S,Tierney P,Araujo N,Mozaffar FH,Pestronk A,Mozaffar T

Cytosolic 5'-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases.
Arthritis care & research68(1)66-71(2016 Jan)
Lloyd TE,Christopher-Stine L,Pinal-Fernandez I,Tiniakou E,Petri M,Baer A,Danoff SK,Pak K,Casciola-Rosen LA,Mammen AL

Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
The Journal of biological chemistry286(40)34567-74(2011 Oct)
Kulkarni SS,Karlsson HK,Szekeres F,Chibalin AV,Krook A,Zierath JR

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NT5C1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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