MED10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED10
Entrez Gene ID	84246
Full Name	mediator complex subunit 10
Synonyms	L6,NUT2,TRG20
General protein information	Preferred Names mediator complex subunit 10 Names mediator of RNA polymerase II transcription subunit 10 TRG-17 TRG-20 mediator of RNA polymerase II transcription, subunit 10 transformation-related gene 17 protein transformation-related gene 20 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p15.31
Summary	MED10 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008].
Disorder MIM:	612382

mRNA and Protein(s)

mRNA	Protein	Name
NM_032286.2	NP_115662.2	mediator of RNA polymerase II transcription subunit 10
NM_032286.3	NP_115662.2	mediator of RNA polymerase II transcription subunit 10

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED10	NP_115662.2
Pan troglodytes (chimpanzee)	MED10	XP_001143506.1
Caenorhabditis elegans (roundworm)	mdt-10	NP_495650.1
Xenopus tropicalis (tropical clawed frog)	med10	NP_001004914.1
	MED10	XP_001082923.1
Mus musculus (house mouse)	Med10	NP_613062.1
Danio rerio (zebrafish)	med10	NP_001017699.1
Drosophila melanogaster (fruit fly)	MED10	NP_001261931.1
Canis lupus familiaris (dog)	MED10	XP_849860.2
Bos taurus (cattle)	MED10	NP_001029928.1
Rattus norvegicus (Norway rat)	Med10	NP_001099567.2
Gallus gallus (chicken)	MED10	XP_004935190.1
Anopheles gambiae (African malaria mosquito)	MED10_ANOGA	XP_316314.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

Determination of the 'critical region' for cat-like cry of Cri-du-chat syndrome and analysis of candidate genes by quantitative PCR.
Wu Q, Niebuhr E, Yang H, Hansen L
European journal of human genetics : EJHG13(4)475-85(2005 Apr)

Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Petroziello J, Yamane A, Westendorf L, Thompson M, McDonagh C, Cerveny C, Law CL, Wahl A, Carter P
Oncogene23(46)7734-45(2004 Oct)

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell14(5)685-91(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04945	NM_032286.3 Latest version!	Homo sapiens mediator complex subunit 10 (MED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04945	NM_032286.2	Homo sapiens mediator complex subunit 10 (MED10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED10 ( NM_032286.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115662.2 Homo sapiens mediator complex subunit 10 (MED10), mRNA.

Price & Availability↑

CloneID	OHu04945
Clone ID Related Accession (Same CDS sequence)	NM_032286.2 , NM_032286.3
Accession Version	NM_032286.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC003353.1, BI597082.1 and AA648258.1. On Jun 25, 2004 this sequence version replaced NM_032286.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.176309.1, SRR1163655.436654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGA AGTTTGACCA CCTAGAGGAG CACCTGGAGA AGTTCGTGGA GAACATTCGG 
CAGCTCGGCA TCATCGTCAG TGACTTCCAG CCCAGCAGCC AGGCCGGGCT CAACCAAAAG 
CTGAATTTTA TTGTTACTGG CTTACAGGAT ATTGACAAGT GCAGACAGCA GCTTCATGAT 
ATTACTGTAC CGTTAGAAGT TTTTGAATAT ATAGATCAAG GTCGAAATCC CCAGCTCTAC 
ACCAAAGAGT GCCTGGAGAG GGCTCTAGCT AAAAATGAGC AAGTTAAAGG CAAGATCGAC 
ACCATGAAGA AATTTAAAAG CCTGTTGATT CAAGAACTTT CTAAAGTATT TCCGGAAGAC 
ATGGCTAAGT ATCGAAGCAT CCGGGGGGAG GATCACCCGC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115662.2
CDS	44..451
Translation	MAEKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS

Target ORF information:

RefSeq Version	NM_032286.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 10 (MED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032286.2

ORF Insert Sequence:

ATGGCGGAGA AGTTTGACCA CCTAGAGGAG CACCTGGAGA AGTTCGTGGA GAACATTCGG 
CAGCTCGGCA TCATCGTCAG TGACTTCCAG CCCAGCAGCC AGGCCGGGCT CAACCAAAAG 
CTGAATTTTA TTGTTACTGG CTTACAGGAT ATTGACAAGT GCAGACAGCA GCTTCATGAT 
ATTACTGTAC CGTTAGAAGT TTTTGAATAT ATAGATCAAG GTCGAAATCC CCAGCTCTAC 
ACCAAAGAGT GCCTGGAGAG GGCTCTAGCT AAAAATGAGC AAGTTAAAGG CAAGATCGAC 
ACCATGAAGA AATTTAAAAG CCTGTTGATT CAAGAACTTT CTAAAGTATT TCCGGAAGAC 
ATGGCTAAGT ATCGAAGCAT CCGGGGGGAG GATCACCCGC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED10 ( NM_032286.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115662.2 Homo sapiens mediator complex subunit 10 (MED10), mRNA.

Price & Availability↑

CloneID	OHu04945
Clone ID Related Accession (Same CDS sequence)	NM_032286.2 , NM_032286.3
Accession Version	NM_032286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093307.5, BC003353.1, BI597082.1, AA648258.1 and AC010635.6. On Nov 22, 2018 this sequence version replaced NM_032286.2. ##Evidence-Data-START## Transcript exon combination :: CB149061.1, SRR5189652.30109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255764.4/ ENSP00000255764.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGA AGTTTGACCA CCTAGAGGAG CACCTGGAGA AGTTCGTGGA GAACATTCGG 
CAGCTCGGCA TCATCGTCAG TGACTTCCAG CCCAGCAGCC AGGCCGGGCT CAACCAAAAG 
CTGAATTTTA TTGTTACTGG CTTACAGGAT ATTGACAAGT GCAGACAGCA GCTTCATGAT 
ATTACTGTAC CGTTAGAAGT TTTTGAATAT ATAGATCAAG GTCGAAATCC CCAGCTCTAC 
ACCAAAGAGT GCCTGGAGAG GGCTCTAGCT AAAAATGAGC AAGTTAAAGG CAAGATCGAC 
ACCATGAAGA AATTTAAAAG CCTGTTGATT CAAGAACTTT CTAAAGTATT TCCGGAAGAC 
ATGGCTAAGT ATCGAAGCAT CCGGGGGGAG GATCACCCGC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115662.2
CDS	65..472
Translation	MAEKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS

Target ORF information:

RefSeq Version	NM_032286.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 10 (MED10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032286.3

ORF Insert Sequence:

ATGGCGGAGA AGTTTGACCA CCTAGAGGAG CACCTGGAGA AGTTCGTGGA GAACATTCGG 
CAGCTCGGCA TCATCGTCAG TGACTTCCAG CCCAGCAGCC AGGCCGGGCT CAACCAAAAG 
CTGAATTTTA TTGTTACTGG CTTACAGGAT ATTGACAAGT GCAGACAGCA GCTTCATGAT 
ATTACTGTAC CGTTAGAAGT TTTTGAATAT ATAGATCAAG GTCGAAATCC CCAGCTCTAC 
ACCAAAGAGT GCCTGGAGAG GGCTCTAGCT AAAAATGAGC AAGTTAAAGG CAAGATCGAC 
ACCATGAAGA AATTTAAAAG CCTGTTGATT CAAGAACTTT CTAAAGTATT TCCGGAAGAC 
ATGGCTAAGT ATCGAAGCAT CCGGGGGGAG GATCACCCGC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

Determination of the 'critical region' for cat-like cry of Cri-du-chat syndrome and analysis of candidate genes by quantitative PCR.
European journal of human genetics : EJHG13(4)475-85(2005 Apr)
Wu Q,Niebuhr E,Yang H,Hansen L

Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer.
Oncogene23(46)7734-45(2004 Oct)
Petroziello J,Yamane A,Westendorf L,Thompson M,McDonagh C,Cerveny C,Law CL,Wahl A,Carter P

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Molecular cell14(5)685-91(2004 Jun)
Sato S,Tomomori-Sato C,Parmely TJ,Florens L,Zybailov B,Swanson SK,Banks CA,Jin J,Cai Y,Washburn MP,Conaway JW,Conaway RC

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MED10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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