CARS1 cDNA ORF clone, Homo sapiens(human)

The following CARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13862 NM_001751.6
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu92568 XM_017018391.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $307.30
$439.00
OHu13803 NM_139273.4
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu13978 NM_001014437.3
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu14615 NM_001194997.2
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $573.30
$819.00
OHu45024 XM_006718341.3
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu103622 XM_024448701.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu103623 XM_024448702.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu103624 XM_024448703.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu103625 XM_024448704.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu103626 XM_024448705.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu92567 XM_017018389.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu92567 XM_017018390.1
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu92569 XM_017018392.2
Latest version!
Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu14615 NM_001194997.1 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $573.30
$819.00
OHu13978 NM_001014437.2 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $587.30
$839.00
OHu92569 XM_017018392.1 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu13803 NM_139273.3 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu13862 NM_001751.5 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu13862
    Clone ID Related Accession (Same CDS sequence) NM_001751.5 , NM_001751.6
    Accession Version NM_001751.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, BC002880.2 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_001751.4. Transcript Variant: This variant (2) uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a longer and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002880.2, SRR1803617.20540.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA
    GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG
    ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001742.1
    CDS85..2331
    Translation

    Target ORF information:

    RefSeq Version NM_001751.5
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001751.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA
    GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG
    ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13862
    Clone ID Related Accession (Same CDS sequence) NM_001751.5 , NM_001751.6
    Accession Version NM_001751.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002880.2. On Jun 2, 2019 this sequence version replaced NM_001751.5. Transcript Variant: This variant (2) uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a longer and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1105396.1, SRR3476690.587542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA
    GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG
    ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001742.1
    CDS57..2303
    Translation

    Target ORF information:

    RefSeq Version NM_001751.6
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001751.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA
    GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG
    ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92568
    Clone ID Related Accession (Same CDS sequence) XM_017018391.1
    Accession Version XM_017018391.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
    GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC
    CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC
    GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC
    AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT
    TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC
    TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT
    CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC
    CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA
    GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA
    GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT
    GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC
    TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA
    GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC
    CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG
    TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA
    AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG CACGGCAGTT GCGGCTGGCC
    TTCCTCATGC ACTCGTGGAA GGACACCCTG GACTACTCCA GCAACACCAT GGAGTCAGCG
    CTTCAATATG AGAAGTTCTT GAATGAGTTT TTCTTAAATG TGAAAGATAT CCTTCGCGCT
    CCTGTTGACA TCACTGGTCA GTTTGAGAAG TGGGGAGAAG AAGAAGCAGA ACTGAATAAG
    AACTTTTATG ACAAGAAGAC AGCAATTCAC AAAGCCCTCT GTGACAATGT TGACACCCGC
    ACCGTCATGG AAGAGATGCG GGCCTTGGTC AGTCAGTGCA ACCTCTATAT GGCAGCCCGG
    AAAGCCGTGA GGAAGAGGCC CAACCAGGCT CTGCTGGAGA ACATCGCCCT GTACCTCACC
    CATATGCTGA AGATCTTTGG GGCCGTAGAA GAGGACAGCT CCCTGGGATT CCCGGTCGGA
    GGGCCTGGAA CCAGCCTCAG TCTCGAGGCC ACAGTCATGC CCTACCTTCA GGTGTTATCA
    GAATTCCGAG AAGGAGTGCG GAAGATTGCC CGAGAGCAAA AAGTCCCTGA GATTCTGCAG
    CTCAGCGATG CCCTGCGGGA CAACATCCTG CCCGAGCTTG GGGTGCGGTT TGAAGACCAC
    GAAGGACTGC CCACAGTGGT GAAACTGGTA GACAGAAACA CCTTATTAAA AGAGAGAGAA
    GAAAAGAGAC GGGTTGAAGA GGAGAAGAGG AAGAAGAAAG AGGAGGCGGC CCGGAGGAAA
    CAGGAACAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATTC CCCCCAGTGA GATGTTCTTG
    TCAGAAACCG ACAAATACTC CAAGTTTGAT GAAAATGGTC TGCCCACACA TGACATGGAG
    GGCAAAGAGC TCAGCAAAGG GCAAGCCAAG AAGCTGAAGA AGCTCTTCGA GGCTCAGGAG
    AAGCTCTACA AGGAATATCT GCAGATGGCC CAGAATGGAA GCTTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873880.1
    CDS608..2578
    Translation

    Target ORF information:

    RefSeq Version XM_017018391.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018391.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
    GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC
    CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC
    GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC
    AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT
    TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC
    TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT
    CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC
    CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA
    GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA
    GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT
    GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC
    TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA
    GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC
    CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG
    TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA
    AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG CACGGCAGTT GCGGCTGGCC
    TTCCTCATGC ACTCGTGGAA GGACACCCTG GACTACTCCA GCAACACCAT GGAGTCAGCG
    CTTCAATATG AGAAGTTCTT GAATGAGTTT TTCTTAAATG TGAAAGATAT CCTTCGCGCT
    CCTGTTGACA TCACTGGTCA GTTTGAGAAG TGGGGAGAAG AAGAAGCAGA ACTGAATAAG
    AACTTTTATG ACAAGAAGAC AGCAATTCAC AAAGCCCTCT GTGACAATGT TGACACCCGC
    ACCGTCATGG AAGAGATGCG GGCCTTGGTC AGTCAGTGCA ACCTCTATAT GGCAGCCCGG
    AAAGCCGTGA GGAAGAGGCC CAACCAGGCT CTGCTGGAGA ACATCGCCCT GTACCTCACC
    CATATGCTGA AGATCTTTGG GGCCGTAGAA GAGGACAGCT CCCTGGGATT CCCGGTCGGA
    GGGCCTGGAA CCAGCCTCAG TCTCGAGGCC ACAGTCATGC CCTACCTTCA GGTGTTATCA
    GAATTCCGAG AAGGAGTGCG GAAGATTGCC CGAGAGCAAA AAGTCCCTGA GATTCTGCAG
    CTCAGCGATG CCCTGCGGGA CAACATCCTG CCCGAGCTTG GGGTGCGGTT TGAAGACCAC
    GAAGGACTGC CCACAGTGGT GAAACTGGTA GACAGAAACA CCTTATTAAA AGAGAGAGAA
    GAAAAGAGAC GGGTTGAAGA GGAGAAGAGG AAGAAGAAAG AGGAGGCGGC CCGGAGGAAA
    CAGGAACAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATTC CCCCCAGTGA GATGTTCTTG
    TCAGAAACCG ACAAATACTC CAAGTTTGAT GAAAATGGTC TGCCCACACA TGACATGGAG
    GGCAAAGAGC TCAGCAAAGG GCAAGCCAAG AAGCTGAAGA AGCTCTTCGA GGCTCAGGAG
    AAGCTCTACA AGGAATATCT GCAGATGGCC CAGAATGGAA GCTTCCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13803
    Clone ID Related Accession (Same CDS sequence) NM_139273.3 , NM_139273.4
    Accession Version NM_139273.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AF288207.1, AI608729.1 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_139273.2. Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.233531.1, SRR1803615.210092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA
    AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_644802.1
    CDS85..2265
    Translation

    Target ORF information:

    RefSeq Version NM_139273.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_139273.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA
    AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13803
    Clone ID Related Accession (Same CDS sequence) NM_139273.3 , NM_139273.4
    Accession Version NM_139273.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AF288207.1 and AI608729.1. On Jun 2, 2019 this sequence version replaced NM_139273.3. Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.269303.1, SRR1803613.181635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA
    AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_644802.1
    CDS57..2237
    Translation

    Target ORF information:

    RefSeq Version NM_139273.4
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_139273.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC
    ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT
    GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT
    GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA
    ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC
    TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC
    CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC
    GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC
    GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG
    ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC
    ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA
    CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG
    AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG
    GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG
    TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA
    AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13978
    Clone ID Related Accession (Same CDS sequence) NM_001014437.3 , NM_001014437.2
    Accession Version NM_001014437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647906.1. On Jun 2, 2019 this sequence version replaced NM_001014437.2. Transcript Variant: This variant (3) includes an additional in-frame exon in the 5' coding region, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) has an additional segment in the N-terminal region and a distinct and longer C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647906.1, SRR1660809.176892.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC
    AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA
    TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014437.1
    CDS57..2552
    Translation

    Target ORF information:

    RefSeq Version NM_001014437.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC
    AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA
    TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13978
    Clone ID Related Accession (Same CDS sequence) NM_001014437.3 , NM_001014437.2
    Accession Version NM_001014437.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-16
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, BX647906.1 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_001014437.1. Transcript Variant: This variant (3) includes an additional in-frame exon in the 5' coding region, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) has an additional segment in the N-terminal region and a distinct and longer C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647906.1, SRR1803615.173119.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC
    AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA
    TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014437.1
    CDS85..2580
    Translation

    Target ORF information:

    RefSeq Version NM_001014437.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC
    AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA
    TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14615
    Clone ID Related Accession (Same CDS sequence) NM_001194997.2 , NM_001194997.1
    Accession Version NM_001194997.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AK298514.1, AC131971.9 and AI432096.1. On Jun 2, 2019 this sequence version replaced NM_001194997.1. Transcript Variant: This variant (5) includes an additional in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (e) that is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.81182.1, SRR1803613.150387.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG
    AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001181926.1
    CDS57..2486
    Translation

    Target ORF information:

    RefSeq Version NM_001194997.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001194997.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG
    AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14615
    Clone ID Related Accession (Same CDS sequence) NM_001194997.2 , NM_001194997.1
    Accession Version NM_001194997.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-16
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AK298514.1, AC131971.9 and AI432096.1. Transcript Variant: This variant (5) includes an additional in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (e) that is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.47536.1, AK298514.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG
    AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001181926.1
    CDS85..2514
    Translation

    Target ORF information:

    RefSeq Version NM_001194997.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001194997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG
    TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT
    GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG
    AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG
    ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG
    AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC
    TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC
    CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA
    GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG
    CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA
    GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT
    GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA
    GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA
    TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG
    AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45024
    Clone ID Related Accession (Same CDS sequence) XM_006718341.3
    Accession Version XM_006718341.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGGTCTGCC CACACATGAC
    ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT
    CAGGAGAAGC TCTACAAGGA ATATCTGCAG ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718404.1
    CDS138..2354
    Translation

    Target ORF information:

    RefSeq Version XM_006718341.3
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718341.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGGTCTGCC CACACATGAC
    ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT
    CAGGAGAAGC TCTACAAGGA ATATCTGCAG ATGGCCCAGA ATGGAAGCTT CCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103622
    Clone ID Related Accession (Same CDS sequence) XM_024448701.1
    Accession Version XM_024448701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA
    CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG
    TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC
    CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG
    TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG
    AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT
    CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG
    GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG
    GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA
    GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG
    GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT
    GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC
    CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG
    GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG
    AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC
    AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT
    GATGAAAATG GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC
    AAGAAGCTGA AGAAGCTCTT CGAGGCTCAG GAGAAGCTCT ACAAGGAATA TCTGCAGATG
    GCCCAGAATG GAAGCTTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304469.1
    CDS57..2660
    Translation

    Target ORF information:

    RefSeq Version XM_024448701.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA
    CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG
    TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC
    CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG
    TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG
    AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT
    CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG
    GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG
    GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA
    GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG
    GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT
    GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC
    CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG
    GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG
    AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC
    AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT
    GATGAAAATG GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC
    AAGAAGCTGA AGAAGCTCTT CGAGGCTCAG GAGAAGCTCT ACAAGGAATA TCTGCAGATG
    GCCCAGAATG GAAGCTTCCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103623
    Clone ID Related Accession (Same CDS sequence) XM_024448702.1
    Accession Version XM_024448702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA
    CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG
    TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC
    CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG
    TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG
    AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT
    CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG
    GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG
    GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA
    GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG
    GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT
    GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC
    CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG
    GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG
    AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC
    AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT
    GATGAAAATG TAAGCATGGT CTGCCCACAC ATGACATGGA GGGCAAAGAG CTCAGCAAAG
    GGCAAGCCAA GAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304470.1
    CDS57..2594
    Translation

    Target ORF information:

    RefSeq Version XM_024448702.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
    GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG
    TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC
    CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC
    TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG
    TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG
    AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA
    ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG
    AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT
    ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG
    AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA
    GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC
    GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG
    GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG
    CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG
    TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC
    TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC
    AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT
    AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT
    CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC
    AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG
    GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG
    GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG
    CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT
    AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA
    CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG
    TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC
    CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG
    TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG
    AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT
    CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG
    GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG
    GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA
    GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG
    GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT
    GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC
    CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG
    GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG
    AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC
    AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT
    GATGAAAATG TAAGCATGGT CTGCCCACAC ATGACATGGA GGGCAAAGAG CTCAGCAAAG
    GGCAAGCCAA GAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103624
    Clone ID Related Accession (Same CDS sequence) XM_024448703.1
    Accession Version XM_024448703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGGTAT ACACTGGCTT CTCCCACAGA CCCCCCAACC ACCTTCTCAT
    ACATGTGTAA TGGCTGTAGC ACTCGGGAGA ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC
    ACAGCACGGC AGTTGCGGCT GGCCTTCCTC ATGCACTCGT GGAAGGACAC CCTGGACTAC
    TCCAGCAACA CCATGGAGTC AGCGCTTCAA TATGAGAAGT TCTTGAATGA GTTTTTCTTA
    AATGTGAAAG ATATCCTTCG CGCTCCTGTT GACATCACTG GTCAGTTTGA GAAGTGGGGA
    GAAGAAGAAG CAGAACTGAA TAAGAACTTT TATGACAAGA AGACAGCAAT TCACAAAGCC
    CTCTGTGACA ATGTTGACAC CCGCACCGTC ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG
    TGCAACCTCT ATATGGCAGC CCGGAAAGCC GTGAGGAAGA GGCCCAACCA GGCTCTGCTG
    GAGAACATCG CCCTGTACCT CACCCATATG CTGAAGATCT TTGGGGCCGT AGAAGAGGAC
    AGCTCCCTGG GATTCCCGGT CGGAGGGCCT GGAACCAGCC TCAGTCTCGA GGCCACAGTC
    ATGCCCTACC TTCAGGTGTT ATCAGAATTC CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG
    CAAAAAGTCC CTGAGATTCT GCAGCTCAGC GATGCCCTGC GGGACAACAT CCTGCCCGAG
    CTTGGGGTGC GGTTTGAAGA CCACGAAGGA CTGCCCACAG TGGTGAAACT GGTAGACAGA
    AACACCTTAT TAAAAGAGAG AGAAGAAAAG AGACGGGTTG AAGAGGAGAA GAGGAAGAAG
    AAAGAGGAGG CGGCCCGGAG GAAACAGGAA CAAGAAGCAG CAAAGCTGGC CAAGATGAAG
    ATTCCCCCCA GTGAGATGTT CTTGTCAGAA ACCGACAAAT ACTCCAAGTT TGATGAAAAT
    GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG
    AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC TACAAGGAAT ATCTGCAGAT GGCCCAGAAT
    GGAAGCTTCC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304471.1
    CDS57..2411
    Translation

    Target ORF information:

    RefSeq Version XM_024448703.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
    CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA
    GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT
    GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG
    TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC
    AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT
    GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA
    AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG
    CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC
    AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT
    ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG
    GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG
    GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC
    GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG
    CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG
    GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT
    GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT
    CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG
    GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG
    TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG
    ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC
    TTGAAAAAGC ACTCAGGTAT ACACTGGCTT CTCCCACAGA CCCCCCAACC ACCTTCTCAT
    ACATGTGTAA TGGCTGTAGC ACTCGGGAGA ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC
    ACAGCACGGC AGTTGCGGCT GGCCTTCCTC ATGCACTCGT GGAAGGACAC CCTGGACTAC
    TCCAGCAACA CCATGGAGTC AGCGCTTCAA TATGAGAAGT TCTTGAATGA GTTTTTCTTA
    AATGTGAAAG ATATCCTTCG CGCTCCTGTT GACATCACTG GTCAGTTTGA GAAGTGGGGA
    GAAGAAGAAG CAGAACTGAA TAAGAACTTT TATGACAAGA AGACAGCAAT TCACAAAGCC
    CTCTGTGACA ATGTTGACAC CCGCACCGTC ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG
    TGCAACCTCT ATATGGCAGC CCGGAAAGCC GTGAGGAAGA GGCCCAACCA GGCTCTGCTG
    GAGAACATCG CCCTGTACCT CACCCATATG CTGAAGATCT TTGGGGCCGT AGAAGAGGAC
    AGCTCCCTGG GATTCCCGGT CGGAGGGCCT GGAACCAGCC TCAGTCTCGA GGCCACAGTC
    ATGCCCTACC TTCAGGTGTT ATCAGAATTC CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG
    CAAAAAGTCC CTGAGATTCT GCAGCTCAGC GATGCCCTGC GGGACAACAT CCTGCCCGAG
    CTTGGGGTGC GGTTTGAAGA CCACGAAGGA CTGCCCACAG TGGTGAAACT GGTAGACAGA
    AACACCTTAT TAAAAGAGAG AGAAGAAAAG AGACGGGTTG AAGAGGAGAA GAGGAAGAAG
    AAAGAGGAGG CGGCCCGGAG GAAACAGGAA CAAGAAGCAG CAAAGCTGGC CAAGATGAAG
    ATTCCCCCCA GTGAGATGTT CTTGTCAGAA ACCGACAAAT ACTCCAAGTT TGATGAAAAT
    GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG
    AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC TACAAGGAAT ATCTGCAGAT GGCCCAGAAT
    GGAAGCTTCC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103625
    Clone ID Related Accession (Same CDS sequence) XM_024448704.1
    Accession Version XM_024448704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGGTAT ACACTGGCTT
    CTCCCACAGA CCCCCCAACC ACCTTCTCAT ACATGTGTAA TGGCTGTAGC ACTCGGGAGA
    ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC ACAGCACGGC AGTTGCGGCT GGCCTTCCTC
    ATGCACTCGT GGAAGGACAC CCTGGACTAC TCCAGCAACA CCATGGAGTC AGCGCTTCAA
    TATGAGAAGT TCTTGAATGA GTTTTTCTTA AATGTGAAAG ATATCCTTCG CGCTCCTGTT
    GACATCACTG GTCAGTTTGA GAAGTGGGGA GAAGAAGAAG CAGAACTGAA TAAGAACTTT
    TATGACAAGA AGACAGCAAT TCACAAAGCC CTCTGTGACA ATGTTGACAC CCGCACCGTC
    ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG TGCAACCTCT ATATGGCAGC CCGGAAAGCC
    GTGAGGAAGA GGCCCAACCA GGCTCTGCTG GAGAACATCG CCCTGTACCT CACCCATATG
    CTGAAGATCT TTGGGGCCGT AGAAGAGGAC AGCTCCCTGG GATTCCCGGT CGGAGGGCCT
    GGAACCAGCC TCAGTCTCGA GGCCACAGTC ATGCCCTACC TTCAGGTGTT ATCAGAATTC
    CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG CAAAAAGTCC CTGAGATTCT GCAGCTCAGC
    GATGCCCTGC GGGACAACAT CCTGCCCGAG CTTGGGGTGC GGTTTGAAGA CCACGAAGGA
    CTGCCCACAG TGGTGAAACT GGTAGACAGA AACACCTTAT TAAAAGAGAG AGAAGAAAAG
    AGACGGGTTG AAGAGGAGAA GAGGAAGAAG AAAGAGGAGG CGGCCCGGAG GAAACAGGAA
    CAAGAAGCAG CAAAGCTGGC CAAGATGAAG ATTCCCCCCA GTGAGATGTT CTTGTCAGAA
    ACCGACAAAT ACTCCAAGTT TGATGAAAAT GGTCTGCCCA CACATGACAT GGAGGGCAAA
    GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC
    TACAAGGAAT ATCTGCAGAT GGCCCAGAAT GGAAGCTTCC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304472.1
    CDS378..2702
    Translation

    Target ORF information:

    RefSeq Version XM_024448704.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGGTAT ACACTGGCTT
    CTCCCACAGA CCCCCCAACC ACCTTCTCAT ACATGTGTAA TGGCTGTAGC ACTCGGGAGA
    ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC ACAGCACGGC AGTTGCGGCT GGCCTTCCTC
    ATGCACTCGT GGAAGGACAC CCTGGACTAC TCCAGCAACA CCATGGAGTC AGCGCTTCAA
    TATGAGAAGT TCTTGAATGA GTTTTTCTTA AATGTGAAAG ATATCCTTCG CGCTCCTGTT
    GACATCACTG GTCAGTTTGA GAAGTGGGGA GAAGAAGAAG CAGAACTGAA TAAGAACTTT
    TATGACAAGA AGACAGCAAT TCACAAAGCC CTCTGTGACA ATGTTGACAC CCGCACCGTC
    ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG TGCAACCTCT ATATGGCAGC CCGGAAAGCC
    GTGAGGAAGA GGCCCAACCA GGCTCTGCTG GAGAACATCG CCCTGTACCT CACCCATATG
    CTGAAGATCT TTGGGGCCGT AGAAGAGGAC AGCTCCCTGG GATTCCCGGT CGGAGGGCCT
    GGAACCAGCC TCAGTCTCGA GGCCACAGTC ATGCCCTACC TTCAGGTGTT ATCAGAATTC
    CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG CAAAAAGTCC CTGAGATTCT GCAGCTCAGC
    GATGCCCTGC GGGACAACAT CCTGCCCGAG CTTGGGGTGC GGTTTGAAGA CCACGAAGGA
    CTGCCCACAG TGGTGAAACT GGTAGACAGA AACACCTTAT TAAAAGAGAG AGAAGAAAAG
    AGACGGGTTG AAGAGGAGAA GAGGAAGAAG AAAGAGGAGG CGGCCCGGAG GAAACAGGAA
    CAAGAAGCAG CAAAGCTGGC CAAGATGAAG ATTCCCCCCA GTGAGATGTT CTTGTCAGAA
    ACCGACAAAT ACTCCAAGTT TGATGAAAAT GGTCTGCCCA CACATGACAT GGAGGGCAAA
    GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC
    TACAAGGAAT ATCTGCAGAT GGCCCAGAAT GGAAGCTTCC AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu103626
    Clone ID Related Accession (Same CDS sequence) XM_024448705.1
    Accession Version XM_024448705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
    GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC
    CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC
    GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC
    AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT
    TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC
    TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT
    CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC
    CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA
    GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA
    GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT
    GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC
    TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA
    GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC
    CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG
    TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA
    AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG GTATACACTG GCTTCTCCCA
    CAGACCCCCC AACCACCTTC TCATACATGT GTAATGGCTG TAGCACTCGG GAGAATAAGG
    GCGTTGGCTG TCTTGTGTCC CCCCACAGCA CGGCAGTTGC GGCTGGCCTT CCTCATGCAC
    TCGTGGAAGG ACACCCTGGA CTACTCCAGC AACACCATGG AGTCAGCGCT TCAATATGAG
    AAGTTCTTGA ATGAGTTTTT CTTAAATGTG AAAGATATCC TTCGCGCTCC TGTTGACATC
    ACTGGTCAGT TTGAGAAGTG GGGAGAAGAA GAAGCAGAAC TGAATAAGAA CTTTTATGAC
    AAGAAGACAG CAATTCACAA AGCCCTCTGT GACAATGTTG ACACCCGCAC CGTCATGGAA
    GAGATGCGGG CCTTGGTCAG TCAGTGCAAC CTCTATATGG CAGCCCGGAA AGCCGTGAGG
    AAGAGGCCCA ACCAGGCTCT GCTGGAGAAC ATCGCCCTGT ACCTCACCCA TATGCTGAAG
    ATCTTTGGGG CCGTAGAAGA GGACAGCTCC CTGGGATTCC CGGTCGGAGG GCCTGGAACC
    AGCCTCAGTC TCGAGGCCAC AGTCATGCCC TACCTTCAGG TGTTATCAGA ATTCCGAGAA
    GGAGTGCGGA AGATTGCCCG AGAGCAAAAA GTCCCTGAGA TTCTGCAGCT CAGCGATGCC
    CTGCGGGACA ACATCCTGCC CGAGCTTGGG GTGCGGTTTG AAGACCACGA AGGACTGCCC
    ACAGTGGTGA AACTGGTAGA CAGAAACACC TTATTAAAAG AGAGAGAAGA AAAGAGACGG
    GTTGAAGAGG AGAAGAGGAA GAAGAAAGAG GAGGCGGCCC GGAGGAAACA GGAACAAGAA
    GCAGCAAAGC TGGCCAAGAT GAAGATTCCC CCCAGTGAGA TGTTCTTGTC AGAAACCGAC
    AAATACTCCA AGTTTGATGA AAATGGTCTG CCCACACATG ACATGGAGGG CAAAGAGCTC
    AGCAAAGGGC AAGCCAAGAA GCTGAAGAAG CTCTTCGAGG CTCAGGAGAA GCTCTACAAG
    GAATATCTGC AGATGGCCCA GAATGGAAGC TTCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304473.1
    CDS455..2533
    Translation

    Target ORF information:

    RefSeq Version XM_024448705.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
    GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC
    CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC
    GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC
    AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT
    TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC
    TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT
    CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC
    CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA
    GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA
    GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT
    GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC
    TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA
    GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC
    CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG
    TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA
    AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG GTATACACTG GCTTCTCCCA
    CAGACCCCCC AACCACCTTC TCATACATGT GTAATGGCTG TAGCACTCGG GAGAATAAGG
    GCGTTGGCTG TCTTGTGTCC CCCCACAGCA CGGCAGTTGC GGCTGGCCTT CCTCATGCAC
    TCGTGGAAGG ACACCCTGGA CTACTCCAGC AACACCATGG AGTCAGCGCT TCAATATGAG
    AAGTTCTTGA ATGAGTTTTT CTTAAATGTG AAAGATATCC TTCGCGCTCC TGTTGACATC
    ACTGGTCAGT TTGAGAAGTG GGGAGAAGAA GAAGCAGAAC TGAATAAGAA CTTTTATGAC
    AAGAAGACAG CAATTCACAA AGCCCTCTGT GACAATGTTG ACACCCGCAC CGTCATGGAA
    GAGATGCGGG CCTTGGTCAG TCAGTGCAAC CTCTATATGG CAGCCCGGAA AGCCGTGAGG
    AAGAGGCCCA ACCAGGCTCT GCTGGAGAAC ATCGCCCTGT ACCTCACCCA TATGCTGAAG
    ATCTTTGGGG CCGTAGAAGA GGACAGCTCC CTGGGATTCC CGGTCGGAGG GCCTGGAACC
    AGCCTCAGTC TCGAGGCCAC AGTCATGCCC TACCTTCAGG TGTTATCAGA ATTCCGAGAA
    GGAGTGCGGA AGATTGCCCG AGAGCAAAAA GTCCCTGAGA TTCTGCAGCT CAGCGATGCC
    CTGCGGGACA ACATCCTGCC CGAGCTTGGG GTGCGGTTTG AAGACCACGA AGGACTGCCC
    ACAGTGGTGA AACTGGTAGA CAGAAACACC TTATTAAAAG AGAGAGAAGA AAAGAGACGG
    GTTGAAGAGG AGAAGAGGAA GAAGAAAGAG GAGGCGGCCC GGAGGAAACA GGAACAAGAA
    GCAGCAAAGC TGGCCAAGAT GAAGATTCCC CCCAGTGAGA TGTTCTTGTC AGAAACCGAC
    AAATACTCCA AGTTTGATGA AAATGGTCTG CCCACACATG ACATGGAGGG CAAAGAGCTC
    AGCAAAGGGC AAGCCAAGAA GCTGAAGAAG CTCTTCGAGG CTCAGGAGAA GCTCTACAAG
    GAATATCTGC AGATGGCCCA GAATGGAAGC TTCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92567
    Clone ID Related Accession (Same CDS sequence) XM_017018389.1 , XM_017018390.1
    Accession Version XM_017018389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA
    CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873878.1
    CDS138..2288
    Translation

    Target ORF information:

    RefSeq Version XM_017018389.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA
    CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92567
    Clone ID Related Accession (Same CDS sequence) XM_017018389.1 , XM_017018390.1
    Accession Version XM_017018390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA
    CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873879.1
    CDS378..2528
    Translation

    Target ORF information:

    RefSeq Version XM_017018390.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
    ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG
    ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC
    TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT
    TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC
    CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG
    GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG
    ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG
    CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC
    AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC
    ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG
    AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC
    TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT
    GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC
    GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC
    CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA
    CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC
    ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG
    TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG
    GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA
    CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG
    CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG
    TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT
    CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG
    AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC
    ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA
    GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC
    CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG
    GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG
    TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT
    CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA
    GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG
    AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG
    AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG
    TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA
    CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92569
    Clone ID Related Accession (Same CDS sequence) XM_017018392.2 , XM_017018392.1
    Accession Version XM_017018392.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
    TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC
    AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG
    AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC
    GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT
    GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG
    CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG
    GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT
    AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG
    AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG
    GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT
    GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG
    GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA
    ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT
    GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG
    CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT
    GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG
    CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG
    TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC
    GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC
    GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG
    TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG
    CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC
    ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA
    GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA
    GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC
    AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC
    TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC
    GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG
    GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC
    CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC
    CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG
    CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA
    TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG
    GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC
    AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG
    GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873881.1
    CDS42..2261
    Translation

    Target ORF information:

    RefSeq Version XM_017018392.2
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018392.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
    TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC
    AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG
    AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC
    GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT
    GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG
    CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG
    GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT
    AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG
    AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG
    GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT
    GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG
    GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA
    ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT
    GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG
    CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT
    GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG
    CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG
    TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC
    GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC
    GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG
    TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG
    CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC
    ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA
    GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA
    GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC
    AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC
    TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC
    GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG
    GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC
    CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC
    CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG
    CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA
    TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG
    GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC
    AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG
    GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92569
    Clone ID Related Accession (Same CDS sequence) XM_017018392.2 , XM_017018392.1
    Accession Version XM_017018392.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product cysteine--tRNA ligase, cytoplasmic isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
    TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC
    AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG
    AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC
    GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT
    GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG
    CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG
    GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT
    AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG
    AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG
    GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT
    GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG
    GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA
    ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT
    GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG
    CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT
    GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG
    CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG
    TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC
    GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC
    GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG
    TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG
    CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC
    ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA
    GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA
    GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC
    AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC
    TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC
    GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG
    GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC
    CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC
    CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG
    CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA
    TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG
    GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC
    AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG
    GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873881.1
    CDS231..2450
    Translation

    Target ORF information:

    RefSeq Version XM_017018392.1
    Organism Homo sapiens(human)
    Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
    TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC
    AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG
    AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC
    GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT
    GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG
    CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG
    GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT
    AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG
    AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG
    GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT
    GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG
    GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA
    ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT
    GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG
    CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT
    GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG
    CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG
    TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC
    GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC
    GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG
    TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG
    CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC
    ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA
    GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA
    GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC
    AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC
    TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC
    GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG
    GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC
    CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC
    CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG
    CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA
    TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG
    GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC
    AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG
    GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.