ADAMTS12 cDNA ORF clone, Homo sapiens(human)

The following ADAMTS12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu102046 NM_001324511.2
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu96110 XM_017009908.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu96111 XM_017009909.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu96108 XM_017009906.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu96109 XM_017009907.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu101976 NM_001324512.2
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu96107 XM_017009905.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu29902 NM_030955.4
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
View Old Accession Versions >>
OHu101976 NM_001324512.1 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu102046 NM_001324511.1 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu29902 NM_030955.3 Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu102046
    Clone ID Related Accession (Same CDS sequence) NM_001324511.1 , NM_001324511.2
    Accession Version NM_001324511.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ250725.1, AC139777.3, BG108002.1, BC139900.1, BQ774480.1 and N93301.1. Transcript Variant: This variant (2) encodes isoform 2. The encoded isoform (2) lacks the furin cleavage site, a portion of the propeptide and most conserved domains compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.266668.1, SRR1163655.428592.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267784 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGGTATTG TGACTCACAT GTCCTCCTGG
    GTTGAAGAAT CTGTTTTGTT CTTTTGGTTG TTTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311440.1
    CDS337..1035
    Translation

    Target ORF information:

    RefSeq Version NM_001324511.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGGTATTG TGACTCACAT GTCCTCCTGG
    GTTGAAGAAT CTGTTTTGTT CTTTTGGTTG TTTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102046
    Clone ID Related Accession (Same CDS sequence) NM_001324511.1 , NM_001324511.2
    Accession Version NM_001324511.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG108002.1, BC139900.1, BQ774480.1, N93301.1 and AC008880.6. On Jun 1, 2019 this sequence version replaced NM_001324511.1. Transcript Variant: This variant (2) encodes isoform 2. The encoded isoform (2) lacks the furin cleavage site, a portion of the propeptide and most conserved domains compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358745.1, SRR1163655.266668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267784 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGGTATTG TGACTCACAT GTCCTCCTGG
    GTTGAAGAAT CTGTTTTGTT CTTTTGGTTG TTTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311440.1
    CDS135..833
    Translation

    Target ORF information:

    RefSeq Version NM_001324511.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324511.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGGTATTG TGACTCACAT GTCCTCCTGG
    GTTGAAGAAT CTGTTTTGTT CTTTTGGTTG TTTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96110
    Clone ID Related Accession (Same CDS sequence) XM_017009908.1
    Accession Version XM_017009908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAGTT TTCTGAGAAA ATGCTGGATG CTGTCATTGA TGGTACCCCT TGCTTTGAAG 
    GCGGCAACAG CAGAAATGTC TGTATTAATG GCATATGTAA GGTATTGGAT GGTTGGCTGT
    GACTATGAGA TCGATTCCAA TGCCACCGAG GATCGCTGCG GTGTGTGCCT GGGAGATGGC
    TCTTCCTGCC AGACTGTGAG AAAGATGTTT AAGCAGAAGG AAGGATCTGG TTATGTTGAC
    ATTGGGCTCA TTCCAAAAGG AGCAAGGGAC ATAAGAGTGA TGGAAATTGA GGGAGCTGGA
    AACTTCCTGG CCATCAGGAG TGAAGATCCT GAAAAATATT ACCTGAATGG AGGGTTTATT
    ATCCAGTGGA ACGGGAACTA TAAGCTGGCA GGGACTGTCT TTCAGTATGA CAGGAAAGGA
    GACCTGGAAA AGCTGATGGC CACAGGTCCC ACCAATGAGT CTGTGTGGAT CCAGCTTCTA
    TTCCAGGTGA CTAACCCTGG CATCAAGTAT GAGTACACAA TCCAGAAAGA TGGCCTTGAC
    AATGATGTTG AGCAGCAGAT GTACTTCTGG CAGTACGGCC ACTGGACAGA GTGCAGTGTG
    ACCTGCGGGA CAGGTATCCG CCGCCAAACT GCCCATTGCA TAAAGAAGGG CCGCGGGATG
    GTGAAAGCTA CATTCTGTGA CCCAGAAACA CAGCCCAATG GGAGACAGAA GAAGTGCCAT
    GAAAAGGCTT GTCCACCCAG GTGGTGGGCA GGGGAGTGGG AAGCATGCTC GGCGACATGC
    GGGCCCCACG GGGAGAAGAA GCGAACCGTG CTGTGCATCC AGACCATGGT CTCTGACGAG
    CAGGCTCTCC CGCCCACAGA CTGCCAGCAC CTGCTGAAGC CCAAGACCCT CCTTTCCTGC
    AACAGAGACA TCCTGTGCCC CTCGGACTGG ACAGTGGGCA ACTGGAGTGA GCTTCACTGG
    TTGGGTCCAA ACACAGTTCG ACACTCCTTC AGCTGTACAT TCTCCTGTGC TCACAGCATC
    TGCTTTTCAA AAGAGCTGAT TTCACAGAGC TGTTTTACAT CATGTTCTGT TTCCTGTGGT
    GGTGGAGTGC GGATTCGCAG TGTCACATGT GCCAAGAACC ATGATGAACC TTGCGATGTG
    ACAAGGAAAC CCAACAGCCG AGCTCTGTGT GGCCTCCAGC AATGCCCTTC TAGCCGGAGA
    GTTCTGAAAC CAAACAAAGG CACTATTTCC AATGGAAAAA ACCCACCAAC ACTAAAGCCC
    GTCCCTCCAC CTACATCCAG GCCCAGAATG CTGACCACAC CCACAGGGCC TGAGTCTATG
    AGCACAAGCA CTCCAGCAAT CAGCAGCCCT AGTCCTACCA CAGCCTCCAA AGAAGGAGAC
    CTGGGTGGGA AACAGTGGCA AGATAGCTCA ACCCAACCTG AGCTGAGCTC TCGCTATCTC
    ATTTCCACTG GAAGCACTTC CCAGCCCATC CTCACTTCCC AATCCTTGAG CATTCAGCCA
    AGTGAGGAAA ATGTTTCCAG TTCAGATACT GGTCCTACCT CGGAGGGAGG CCTTGTAGCT
    ACAACAACAA GTGGTTCTGG CTTGTCATCT TCCCGCAACC CTATCACTTG GCCTGTGACT
    CCATTTTACA ATACCTTGAC CAAAGGTCCA GAAATGGAGA TTCACAGTGG CTCAGGGGAA
    GAAAGAGAAC AGCCTGAGGA CAAAGATGAA AGCAATCCTG TAATATGGAC CAAGATCAGA
    GTACCTGGAA ATGACGCTCC AGTGGAAAGT ACAGAAATGC CACTTGCACC TCCACTAACA
    CCAGATCTCA GCAGGGAGTC CTGGTGGCCA CCCTTCAGCA CAGTAATGGA AGGACTGCTC
    CCCAGCCAAA GGCCCACTAC TTCCGAAACT GGGACACCCA GAGTTGAGGG GATGGTTACT
    GAAAAGCCAG CCAACACTCT GCTCCCTCTG GGAGGAGACC ACCAGCCAGA ACCCTCAGGA
    AAGACGGCAA ACCGTAACCA CCTGAAACTT CCAAACAACA TGAACCAAAC AAAAAGTTCT
    GAACCAGTCC TGACTGAGGA GGATGCAACA AGTCTGATTA CTGAGGGCTT TTTGCTAAAT
    GCCTCCAATT ACAAGCAGCT CACAAACGGC CACGGCTCTG CACACTGGAT CGTCGGAAAC
    TGGAGCGAGT GCTCCACCAC ATGTGGCCTG GGGGCCTACT GGAGAAGGGT GGAGTGCAGC
    ACCCAGATGG ATTCTGACTG TGCGGCCATC CAGAGACCTG ACCCTGCAAA AAGATGCCAC
    CTCCGTCCCT GTGCTGGCTG GAAAGTGGGA AACTGGAGCA AGTGCTCCAG AAACTGCAGT
    GGGGGCTTCA AGATACGCGA GATTCAGTGC GTGGACAGCC GGGACCACCG GAACCTGAGG
    CCATTTCACT GCCAGTTCCT GGCCGGCATT CCTCCCCCAT TGAGCATGAG CTGTAACCCG
    GAGCCCTGTG AGGCGTGGCA GGTGGAGCCT TGGAGCCAGT GCTCCAGGTC CTGTGGAGGT
    GGAGTTCAGG AGAGAGGAGT GTTCTGTCCA GGAGGCCTCT GTGATTGGAC AAAAAGACCC
    ACATCCACCA TGTCTTGCAA TGAGCACCTG TGCTGTCACT GGGCCACTGG GAACTGGGAC
    CTGTGTTCCA CTTCCTGTGG AGGTGGCTTT CAGAAGAGGA CTGTCCAATG TGTGCCCTCA
    GAGGGCAATA AAACTGAAGA CCAAGACCAA TGTCTATGTG ATCACAAACC CAGACCTCCA
    GAATTCAAAA AATGCAACCA GCAGGCCTGC AAGAAAAGTG CCGATTTACT TTGCACTAAG
    GACAAACTGT CAGCCAGTTT CTGCCAGACA CTGAAAGCCA TGAAGAAATG TTCTGTGCCC
    ACCGTGAGGG CTGAGTGCTG CTTCTCGTGT CCCCAGACAC ACATCACACA CACCCAAAGG
    CAAAGAAGGC AACGGTTGCT CCAAAAGTCA AAAGAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865397.1
    CDS124..3105
    Translation

    Target ORF information:

    RefSeq Version XM_017009908.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGCCAGTT TTCTGAGAAA ATGCTGGATG CTGTCATTGA TGGTACCCCT TGCTTTGAAG 
    GCGGCAACAG CAGAAATGTC TGTATTAATG GCATATGTAA GGTATTGGAT GGTTGGCTGT
    GACTATGAGA TCGATTCCAA TGCCACCGAG GATCGCTGCG GTGTGTGCCT GGGAGATGGC
    TCTTCCTGCC AGACTGTGAG AAAGATGTTT AAGCAGAAGG AAGGATCTGG TTATGTTGAC
    ATTGGGCTCA TTCCAAAAGG AGCAAGGGAC ATAAGAGTGA TGGAAATTGA GGGAGCTGGA
    AACTTCCTGG CCATCAGGAG TGAAGATCCT GAAAAATATT ACCTGAATGG AGGGTTTATT
    ATCCAGTGGA ACGGGAACTA TAAGCTGGCA GGGACTGTCT TTCAGTATGA CAGGAAAGGA
    GACCTGGAAA AGCTGATGGC CACAGGTCCC ACCAATGAGT CTGTGTGGAT CCAGCTTCTA
    TTCCAGGTGA CTAACCCTGG CATCAAGTAT GAGTACACAA TCCAGAAAGA TGGCCTTGAC
    AATGATGTTG AGCAGCAGAT GTACTTCTGG CAGTACGGCC ACTGGACAGA GTGCAGTGTG
    ACCTGCGGGA CAGGTATCCG CCGCCAAACT GCCCATTGCA TAAAGAAGGG CCGCGGGATG
    GTGAAAGCTA CATTCTGTGA CCCAGAAACA CAGCCCAATG GGAGACAGAA GAAGTGCCAT
    GAAAAGGCTT GTCCACCCAG GTGGTGGGCA GGGGAGTGGG AAGCATGCTC GGCGACATGC
    GGGCCCCACG GGGAGAAGAA GCGAACCGTG CTGTGCATCC AGACCATGGT CTCTGACGAG
    CAGGCTCTCC CGCCCACAGA CTGCCAGCAC CTGCTGAAGC CCAAGACCCT CCTTTCCTGC
    AACAGAGACA TCCTGTGCCC CTCGGACTGG ACAGTGGGCA ACTGGAGTGA GCTTCACTGG
    TTGGGTCCAA ACACAGTTCG ACACTCCTTC AGCTGTACAT TCTCCTGTGC TCACAGCATC
    TGCTTTTCAA AAGAGCTGAT TTCACAGAGC TGTTTTACAT CATGTTCTGT TTCCTGTGGT
    GGTGGAGTGC GGATTCGCAG TGTCACATGT GCCAAGAACC ATGATGAACC TTGCGATGTG
    ACAAGGAAAC CCAACAGCCG AGCTCTGTGT GGCCTCCAGC AATGCCCTTC TAGCCGGAGA
    GTTCTGAAAC CAAACAAAGG CACTATTTCC AATGGAAAAA ACCCACCAAC ACTAAAGCCC
    GTCCCTCCAC CTACATCCAG GCCCAGAATG CTGACCACAC CCACAGGGCC TGAGTCTATG
    AGCACAAGCA CTCCAGCAAT CAGCAGCCCT AGTCCTACCA CAGCCTCCAA AGAAGGAGAC
    CTGGGTGGGA AACAGTGGCA AGATAGCTCA ACCCAACCTG AGCTGAGCTC TCGCTATCTC
    ATTTCCACTG GAAGCACTTC CCAGCCCATC CTCACTTCCC AATCCTTGAG CATTCAGCCA
    AGTGAGGAAA ATGTTTCCAG TTCAGATACT GGTCCTACCT CGGAGGGAGG CCTTGTAGCT
    ACAACAACAA GTGGTTCTGG CTTGTCATCT TCCCGCAACC CTATCACTTG GCCTGTGACT
    CCATTTTACA ATACCTTGAC CAAAGGTCCA GAAATGGAGA TTCACAGTGG CTCAGGGGAA
    GAAAGAGAAC AGCCTGAGGA CAAAGATGAA AGCAATCCTG TAATATGGAC CAAGATCAGA
    GTACCTGGAA ATGACGCTCC AGTGGAAAGT ACAGAAATGC CACTTGCACC TCCACTAACA
    CCAGATCTCA GCAGGGAGTC CTGGTGGCCA CCCTTCAGCA CAGTAATGGA AGGACTGCTC
    CCCAGCCAAA GGCCCACTAC TTCCGAAACT GGGACACCCA GAGTTGAGGG GATGGTTACT
    GAAAAGCCAG CCAACACTCT GCTCCCTCTG GGAGGAGACC ACCAGCCAGA ACCCTCAGGA
    AAGACGGCAA ACCGTAACCA CCTGAAACTT CCAAACAACA TGAACCAAAC AAAAAGTTCT
    GAACCAGTCC TGACTGAGGA GGATGCAACA AGTCTGATTA CTGAGGGCTT TTTGCTAAAT
    GCCTCCAATT ACAAGCAGCT CACAAACGGC CACGGCTCTG CACACTGGAT CGTCGGAAAC
    TGGAGCGAGT GCTCCACCAC ATGTGGCCTG GGGGCCTACT GGAGAAGGGT GGAGTGCAGC
    ACCCAGATGG ATTCTGACTG TGCGGCCATC CAGAGACCTG ACCCTGCAAA AAGATGCCAC
    CTCCGTCCCT GTGCTGGCTG GAAAGTGGGA AACTGGAGCA AGTGCTCCAG AAACTGCAGT
    GGGGGCTTCA AGATACGCGA GATTCAGTGC GTGGACAGCC GGGACCACCG GAACCTGAGG
    CCATTTCACT GCCAGTTCCT GGCCGGCATT CCTCCCCCAT TGAGCATGAG CTGTAACCCG
    GAGCCCTGTG AGGCGTGGCA GGTGGAGCCT TGGAGCCAGT GCTCCAGGTC CTGTGGAGGT
    GGAGTTCAGG AGAGAGGAGT GTTCTGTCCA GGAGGCCTCT GTGATTGGAC AAAAAGACCC
    ACATCCACCA TGTCTTGCAA TGAGCACCTG TGCTGTCACT GGGCCACTGG GAACTGGGAC
    CTGTGTTCCA CTTCCTGTGG AGGTGGCTTT CAGAAGAGGA CTGTCCAATG TGTGCCCTCA
    GAGGGCAATA AAACTGAAGA CCAAGACCAA TGTCTATGTG ATCACAAACC CAGACCTCCA
    GAATTCAAAA AATGCAACCA GCAGGCCTGC AAGAAAAGTG CCGATTTACT TTGCACTAAG
    GACAAACTGT CAGCCAGTTT CTGCCAGACA CTGAAAGCCA TGAAGAAATG TTCTGTGCCC
    ACCGTGAGGG CTGAGTGCTG CTTCTCGTGT CCCCAGACAC ACATCACACA CACCCAAAGG
    CAAAGAAGGC AACGGTTGCT CCAAAAGTCA AAAGAACTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96111
    Clone ID Related Accession (Same CDS sequence) XM_017009909.1
    Accession Version XM_017009909.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCATTGC AAAAGTATTC TTTGTGTAAG GTTGTGATAG CCTTTGTGCA AGGTTGTGGT 
    GTTTGCAGAG GCTTTTTCAT CATCAGGCAT ACAAGGATGG TTGGCTGTGA CTATGAGATC
    GATTCCAATG CCACCGAGGA TCGCTGCGGT GTGTGCCTGG GAGATGGCTC TTCCTGCCAG
    ACTGTGAGAA AGATGTTTAA GCAGAAGGAA GGATCTGGTT ATGTTGACAT TGGGCTCATT
    CCAAAAGGAG CAAGGGACAT AAGAGTGATG GAAATTGAGG GAGCTGGAAA CTTCCTGGCC
    ATCAGGAGTG AAGATCCTGA AAAATATTAC CTGAATGGAG GGTTTATTAT CCAGTGGAAC
    GGGAACTATA AGCTGGCAGG GACTGTCTTT CAGTATGACA GGAAAGGAGA CCTGGAAAAG
    CTGATGGCCA CAGGTCCCAC CAATGAGTCT GTGTGGATCC AGCTTCTATT CCAGGTGACT
    AACCCTGGCA TCAAGTATGA GTACACAATC CAGAAAGATG GCCTTGACAA TGATGTTGAG
    CAGCAGATGT ACTTCTGGCA GTACGGCCAC TGGACAGAGT GCAGTGTGAC CTGCGGGACA
    GGTATCCGCC GCCAAACTGC CCATTGCATA AAGAAGGGCC GCGGGATGGT GAAAGCTACA
    TTCTGTGACC CAGAAACACA GCCCAATGGG AGACAGAAGA AGTGCCATGA AAAGGCTTGT
    CCACCCAGGT GGTGGGCAGG GGAGTGGGAA GCATGCTCGG CGACATGCGG GCCCCACGGG
    GAGAAGAAGC GAACCGTGCT GTGCATCCAG ACCATGGTCT CTGACGAGCA GGCTCTCCCG
    CCCACAGACT GCCAGCACCT GCTGAAGCCC AAGACCCTCC TTTCCTGCAA CAGAGACATC
    CTGTGCCCCT CGGACTGGAC AGTGGGCAAC TGGAGTGAGC TTCACTGGTT GGGTCCAAAC
    ACAGTTCGAC ACTCCTTCAG CTGTACATTC TCCTGTGCTC ACAGCATCTG CTTTTCAAAA
    GAGCTGATTT CACAGAGCTG TTTTACATCA TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865398.1
    CDS50..3019
    Translation

    Target ORF information:

    RefSeq Version XM_017009909.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009909.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCATTGC AAAAGTATTC TTTGTGTAAG GTTGTGATAG CCTTTGTGCA AGGTTGTGGT 
    GTTTGCAGAG GCTTTTTCAT CATCAGGCAT ACAAGGATGG TTGGCTGTGA CTATGAGATC
    GATTCCAATG CCACCGAGGA TCGCTGCGGT GTGTGCCTGG GAGATGGCTC TTCCTGCCAG
    ACTGTGAGAA AGATGTTTAA GCAGAAGGAA GGATCTGGTT ATGTTGACAT TGGGCTCATT
    CCAAAAGGAG CAAGGGACAT AAGAGTGATG GAAATTGAGG GAGCTGGAAA CTTCCTGGCC
    ATCAGGAGTG AAGATCCTGA AAAATATTAC CTGAATGGAG GGTTTATTAT CCAGTGGAAC
    GGGAACTATA AGCTGGCAGG GACTGTCTTT CAGTATGACA GGAAAGGAGA CCTGGAAAAG
    CTGATGGCCA CAGGTCCCAC CAATGAGTCT GTGTGGATCC AGCTTCTATT CCAGGTGACT
    AACCCTGGCA TCAAGTATGA GTACACAATC CAGAAAGATG GCCTTGACAA TGATGTTGAG
    CAGCAGATGT ACTTCTGGCA GTACGGCCAC TGGACAGAGT GCAGTGTGAC CTGCGGGACA
    GGTATCCGCC GCCAAACTGC CCATTGCATA AAGAAGGGCC GCGGGATGGT GAAAGCTACA
    TTCTGTGACC CAGAAACACA GCCCAATGGG AGACAGAAGA AGTGCCATGA AAAGGCTTGT
    CCACCCAGGT GGTGGGCAGG GGAGTGGGAA GCATGCTCGG CGACATGCGG GCCCCACGGG
    GAGAAGAAGC GAACCGTGCT GTGCATCCAG ACCATGGTCT CTGACGAGCA GGCTCTCCCG
    CCCACAGACT GCCAGCACCT GCTGAAGCCC AAGACCCTCC TTTCCTGCAA CAGAGACATC
    CTGTGCCCCT CGGACTGGAC AGTGGGCAAC TGGAGTGAGC TTCACTGGTT GGGTCCAAAC
    ACAGTTCGAC ACTCCTTCAG CTGTACATTC TCCTGTGCTC ACAGCATCTG CTTTTCAAAA
    GAGCTGATTT CACAGAGCTG TTTTACATCA TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96108
    Clone ID Related Accession (Same CDS sequence) XM_017009906.1
    Accession Version XM_017009906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCAGGAG GGACTCAGAA CTGGAGCTTT GCAAATAGAA TGAAAGGACA AGAGGACAAG 
    CAACTTCTCT ACAGACTTCA CCCAGTCCCT ATGCCAGATA GCAACTTCCC TGGGAGTTTC
    GTCTATCATC TTAGCAGAGC ATACAGTGTT AACATCTCCC AGAAGCAAGA GCTATGGCGG
    GAGAAGTGGG AGAGGCACAA CTTGCCAAGC AGAAGCCTCT CTCGGCGTTC CATCAGCAAG
    GAGAGATGGG TGGAGACACT GGTGGTGGCC GACACAAAGA TGATTGAATA CCATGGGAGT
    GAGAATGTGG AGTCCTACAT CCTCACCATC ATGAACATGG TCACTGGGTT GTTCCATAAC
    CCAAGCATTG GCAATGCAAT TCACATTGTT GTGGTTCGGC TCATTCTACT CGAAGAAGAA
    GAGCAAGGAC TGAAAATAGT TCACCATGCA GAAAAGACAC TGTCTAGCTT CTGCAAGTGG
    CAGAAGAGTA TCAATCCCAA GAGTGACCTC AATCCTGTTC ATCACGACGT GGCTGTCCTT
    CTCACCAGAA AGGACATCTG TGCTGGTTTC AATCGCCCCT GCGAGACCCT GGGCCTGTCT
    CACCTTTCAG GAATGTGTCA GCCTCACCGC AGTTGTAACA TCAATGAAGA TTCGGGACTC
    CCTCTGGCTT TCACAATTGC CCATGAGCTA GGACACAGCT TCGGCATCCA GCATGATGGG
    AAAGAAAATG ACTGTGAGCC TGTGGGCAGA CATCCGTACA TCATGTCCCG CCAGCTCCAG
    TACGATCCCA CTCCGCTGAC ATGGTCCAAG TGCAGCGAGG AGTACATCAC CCGCTTCTTG
    GACCGAGGCT GGGGGTTCTG TCTTGATGAC ATACCTAAAA AGAAAGGCTT GAAGTCCAAG
    GTCATTGCCC CCGGAGTGAT CTATGATGTT CACCACCAGT GCCAGCTACA ATATGGACCC
    AATGCTACCT TCTGCCAGGA AGTAGAAAAC GTCTGCCAGA CACTGTGGTG CTCCGTGAAG
    GGCTTTTGTC GCTCTAAGCT GGACGCTGCT GCAGATGGAA CTCAATGTGG TGAGAAGAAG
    TGGTGTATGG CAGGCAAGTG CATCACAGTG GGGAAGAAAC CAGAGAGCAT TCCTGGAGGC
    TGGGGCCGCT GGTCACCCTG GTCCCACTGT TCCAGGACCT GTGGGGCTGG AGTCCAGAGC
    GCAGAGAGGC TCTGCAACAA CCCCGAGCCA AAGTTTGGAG GGAAATATTG CACTGGAGAA
    AGAAAACGCT ATCGCTTGTG CAACGTCCAC CCCTGTCGCT CAGAGGCACC AACATTTCGG
    CAGATGCAGT GCAGTGAATT TGACACTGTT CCCTACAAGA ATGAACTCTA CCACTGGTTT
    CCCATTTTTA ACCCAGCACA TCCTTGTGAG CTCTACTGCC GACCCATAGA TGGCCAGTTT
    TCTGAGAAAA TGCTGGATGC TGTCATTGAT GGTACCCCTT GCTTTGAAGG CGGCAACAGC
    AGAAATGTCT GTATTAATGG CATATGTAAG ATGGTTGGCT GTGACTATGA GATCGATTCC
    AATGCCACCG AGGATCGCTG CGGTGTGTGC CTGGGAGATG GCTCTTCCTG CCAGACTGTG
    AGAAAGATGT TTAAGCAGAA GGAAGGATCT GGTTATGTTG ACATTGGGCT CATTCCAAAA
    GGAGCAAGGG ACATAAGAGT GATGGAAATT GAGGGAGCTG GAAACTTCCT GGCCATCAGG
    AGTGAAGATC CTGAAAAATA TTACCTGAAT GGAGGGTTTA TTATCCAGTG GAACGGGAAC
    TATAAGCTGG CAGGGACTGT CTTTCAGTAT GACAGGAAAG GAGACCTGGA AAAGCTGATG
    GCCACAGGTC CCACCAATGA GTCTGTGTGG ATCCAGCTTC TATTCCAGGT GACTAACCCT
    GGCATCAAGT ATGAGTACAC AATCCAGAAA GATGGCCTTG ACAATGATGT TGAGCAGCAG
    ATGTACTTCT GGCAGTACGG CCACTGGACA GAGTGCAGTG TGACCTGCGG GACAGGTATC
    CGCCGCCAAA CTGCCCATTG CATAAAGAAG GGCCGCGGGA TGGTGAAAGC TACATTCTGT
    GACCCAGAAA CACAGCCCAA TGGGAGACAG AAGAAGTGCC ATGAAAAGGC TTGTCCACCC
    AGGTGGTGGG CAGGGGAGTG GGAAGCATGC TCGGCGACAT GCGGGCCCCA CGGGGAGAAG
    AAGCGAACCG TGCTGTGCAT CCAGACCATG GTCTCTGACG AGCAGGCTCT CCCGCCCACA
    GACTGCCAGC ACCTGCTGAA GCCCAAGACC CTCCTTTCCT GCAACAGAGA CATCCTGTGC
    CCCTCGGACT GGACAGTGGG CAACTGGAGT GAGCTTCACT GGTTGGGTCC AAACACAGTT
    CGACACTCCT TCAGCTGTAC ATTCTCCTGT GCTCACAGCA TCTGCTTTTC AAAAGAGCTG
    ATTTCACAGA GCTGTTTTAC ATCATGTTCT GTTTCCTGTG GTGGTGGAGT GCGGATTCGC
    AGTGTCACAT GTGCCAAGAA CCATGATGAA CCTTGCGATG TGACAAGGAA ACCCAACAGC
    CGAGCTCTGT GTGGCCTCCA GCAATGCCCT TCTAGCCGGA GAGTTCTGAA ACCAAACAAA
    GGCACTATTT CCAATGGAAA AAACCCACCA ACACTAAAGC CCGTCCCTCC ACCTACATCC
    AGGCCCAGAA TGCTGACCAC ACCCACAGGG CCTGAGTCTA TGAGCACAAG CACTCCAGCA
    ATCAGCAGCC CTAGTCCTAC CACAGCCTCC AAAGAAGGAG ACCTGGGTGG GAAACAGTGG
    CAAGATAGCT CAACCCAACC TGAGCTGAGC TCTCGCTATC TCATTTCCAC TGGAAGCACT
    TCCCAGCCCA TCCTCACTTC CCAATCCTTG AGCATTCAGC CAAGTGAGGA AAATGTTTCC
    AGTTCAGATA CTGGTCCTAC CTCGGAGGGA GGCCTTGTAG CTACAACAAC AAGTGGTTCT
    GGCTTGTCAT CTTCCCGCAA CCCTATCACT TGGCCTGTGA CTCCATTTTA CAATACCTTG
    ACCAAAGGTC CAGAAATGGA GATTCACAGT GGCTCAGGGG AAGAAAGAGA ACAGCCTGAG
    GACAAAGATG AAAGCAATCC TGTAATATGG ACCAAGATCA GAGTACCTGG AAATGACGCT
    CCAGTGGAAA GTACAGAAAT GCCACTTGCA CCTCCACTAA CACCAGATCT CAGCAGGGAG
    TCCTGGTGGC CACCCTTCAG CACAGTAATG GAAGGACTGC TCCCCAGCCA AAGGCCCACT
    ACTTCCGAAA CTGGGACACC CAGAGTTGAG GGGATGGTTA CTGAAAAGCC AGCCAACACT
    CTGCTCCCTC TGGGAGGAGA CCACCAGCCA GAACCCTCAG GAAAGACGGC AAACCGTAAC
    CACCTGAAAC TTCCAAACAA CATGAACCAA ACAAAAAGTT CTGAACCAGT CCTGACTGAG
    GAGGATGCAA CAAGTCTGAT TACTGAGGGC TTTTTGCTAA ATGCCTCCAA TTACAAGCAG
    CTCACAAACG GCCACGGCTC TGCACACTGG ATCGTCGGAA ACTGGAGCGA GTGCTCCACC
    ACATGTGGCC TGGGGGCCTA CTGGAGAAGG GTGGAGTGCA GCACCCAGAT GGATTCTGAC
    TGTGCGGCCA TCCAGAGACC TGACCCTGCA AAAAGATGCC ACCTCCGTCC CTGTGCTGGC
    TGGAAAGTGG GAAACTGGAG CAAGTGCTCC AGAAACTGCA GTGGGGGCTT CAAGATACGC
    GAGATTCAGT GCGTGGACAG CCGGGACCAC CGGAACCTGA GGCCATTTCA CTGCCAGTTC
    CTGGCCGGCA TTCCTCCCCC ATTGAGCATG AGCTGTAACC CGGAGCCCTG TGAGGCGTGG
    CAGGTGGAGC CTTGGAGCCA GTGCTCCAGG TCCTGTGGAG GTGGAGTTCA GGAGAGAGGA
    GTGTTCTGTC CAGGAGGCCT CTGTGATTGG ACAAAAAGAC CCACATCCAC CATGTCTTGC
    AATGAGCACC TGTGCTGTCA CTGGGCCACT GGGAACTGGG ACCTGTGTTC CACTTCCTGT
    GGAGGTGGCT TTCAGAAGAG GACTGTCCAA TGTGTGCCCT CAGAGGGCAA TAAAACTGAA
    GACCAAGACC AATGTCTATG TGATCACAAA CCCAGACCTC CAGAATTCAA AAAATGCAAC
    CAGCAGGCCT GCAAGAAAAG TGCCGATTTA CTTTGCACTA AGGACAAACT GTCAGCCAGT
    TTCTGCCAGA CACTGAAAGC CATGAAGAAA TGTTCTGTGC CCACCGTGAG GGCTGAGTGC
    TGCTTCTCGT GTCCCCAGAC ACACATCACA CACACCCAAA GGCAAAGAAG GCAACGGTTG
    CTCCAAAAGT CAAAAGAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865395.1
    CDS218..4621
    Translation

    Target ORF information:

    RefSeq Version XM_017009906.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCAGGAG GGACTCAGAA CTGGAGCTTT GCAAATAGAA TGAAAGGACA AGAGGACAAG 
    CAACTTCTCT ACAGACTTCA CCCAGTCCCT ATGCCAGATA GCAACTTCCC TGGGAGTTTC
    GTCTATCATC TTAGCAGAGC ATACAGTGTT AACATCTCCC AGAAGCAAGA GCTATGGCGG
    GAGAAGTGGG AGAGGCACAA CTTGCCAAGC AGAAGCCTCT CTCGGCGTTC CATCAGCAAG
    GAGAGATGGG TGGAGACACT GGTGGTGGCC GACACAAAGA TGATTGAATA CCATGGGAGT
    GAGAATGTGG AGTCCTACAT CCTCACCATC ATGAACATGG TCACTGGGTT GTTCCATAAC
    CCAAGCATTG GCAATGCAAT TCACATTGTT GTGGTTCGGC TCATTCTACT CGAAGAAGAA
    GAGCAAGGAC TGAAAATAGT TCACCATGCA GAAAAGACAC TGTCTAGCTT CTGCAAGTGG
    CAGAAGAGTA TCAATCCCAA GAGTGACCTC AATCCTGTTC ATCACGACGT GGCTGTCCTT
    CTCACCAGAA AGGACATCTG TGCTGGTTTC AATCGCCCCT GCGAGACCCT GGGCCTGTCT
    CACCTTTCAG GAATGTGTCA GCCTCACCGC AGTTGTAACA TCAATGAAGA TTCGGGACTC
    CCTCTGGCTT TCACAATTGC CCATGAGCTA GGACACAGCT TCGGCATCCA GCATGATGGG
    AAAGAAAATG ACTGTGAGCC TGTGGGCAGA CATCCGTACA TCATGTCCCG CCAGCTCCAG
    TACGATCCCA CTCCGCTGAC ATGGTCCAAG TGCAGCGAGG AGTACATCAC CCGCTTCTTG
    GACCGAGGCT GGGGGTTCTG TCTTGATGAC ATACCTAAAA AGAAAGGCTT GAAGTCCAAG
    GTCATTGCCC CCGGAGTGAT CTATGATGTT CACCACCAGT GCCAGCTACA ATATGGACCC
    AATGCTACCT TCTGCCAGGA AGTAGAAAAC GTCTGCCAGA CACTGTGGTG CTCCGTGAAG
    GGCTTTTGTC GCTCTAAGCT GGACGCTGCT GCAGATGGAA CTCAATGTGG TGAGAAGAAG
    TGGTGTATGG CAGGCAAGTG CATCACAGTG GGGAAGAAAC CAGAGAGCAT TCCTGGAGGC
    TGGGGCCGCT GGTCACCCTG GTCCCACTGT TCCAGGACCT GTGGGGCTGG AGTCCAGAGC
    GCAGAGAGGC TCTGCAACAA CCCCGAGCCA AAGTTTGGAG GGAAATATTG CACTGGAGAA
    AGAAAACGCT ATCGCTTGTG CAACGTCCAC CCCTGTCGCT CAGAGGCACC AACATTTCGG
    CAGATGCAGT GCAGTGAATT TGACACTGTT CCCTACAAGA ATGAACTCTA CCACTGGTTT
    CCCATTTTTA ACCCAGCACA TCCTTGTGAG CTCTACTGCC GACCCATAGA TGGCCAGTTT
    TCTGAGAAAA TGCTGGATGC TGTCATTGAT GGTACCCCTT GCTTTGAAGG CGGCAACAGC
    AGAAATGTCT GTATTAATGG CATATGTAAG ATGGTTGGCT GTGACTATGA GATCGATTCC
    AATGCCACCG AGGATCGCTG CGGTGTGTGC CTGGGAGATG GCTCTTCCTG CCAGACTGTG
    AGAAAGATGT TTAAGCAGAA GGAAGGATCT GGTTATGTTG ACATTGGGCT CATTCCAAAA
    GGAGCAAGGG ACATAAGAGT GATGGAAATT GAGGGAGCTG GAAACTTCCT GGCCATCAGG
    AGTGAAGATC CTGAAAAATA TTACCTGAAT GGAGGGTTTA TTATCCAGTG GAACGGGAAC
    TATAAGCTGG CAGGGACTGT CTTTCAGTAT GACAGGAAAG GAGACCTGGA AAAGCTGATG
    GCCACAGGTC CCACCAATGA GTCTGTGTGG ATCCAGCTTC TATTCCAGGT GACTAACCCT
    GGCATCAAGT ATGAGTACAC AATCCAGAAA GATGGCCTTG ACAATGATGT TGAGCAGCAG
    ATGTACTTCT GGCAGTACGG CCACTGGACA GAGTGCAGTG TGACCTGCGG GACAGGTATC
    CGCCGCCAAA CTGCCCATTG CATAAAGAAG GGCCGCGGGA TGGTGAAAGC TACATTCTGT
    GACCCAGAAA CACAGCCCAA TGGGAGACAG AAGAAGTGCC ATGAAAAGGC TTGTCCACCC
    AGGTGGTGGG CAGGGGAGTG GGAAGCATGC TCGGCGACAT GCGGGCCCCA CGGGGAGAAG
    AAGCGAACCG TGCTGTGCAT CCAGACCATG GTCTCTGACG AGCAGGCTCT CCCGCCCACA
    GACTGCCAGC ACCTGCTGAA GCCCAAGACC CTCCTTTCCT GCAACAGAGA CATCCTGTGC
    CCCTCGGACT GGACAGTGGG CAACTGGAGT GAGCTTCACT GGTTGGGTCC AAACACAGTT
    CGACACTCCT TCAGCTGTAC ATTCTCCTGT GCTCACAGCA TCTGCTTTTC AAAAGAGCTG
    ATTTCACAGA GCTGTTTTAC ATCATGTTCT GTTTCCTGTG GTGGTGGAGT GCGGATTCGC
    AGTGTCACAT GTGCCAAGAA CCATGATGAA CCTTGCGATG TGACAAGGAA ACCCAACAGC
    CGAGCTCTGT GTGGCCTCCA GCAATGCCCT TCTAGCCGGA GAGTTCTGAA ACCAAACAAA
    GGCACTATTT CCAATGGAAA AAACCCACCA ACACTAAAGC CCGTCCCTCC ACCTACATCC
    AGGCCCAGAA TGCTGACCAC ACCCACAGGG CCTGAGTCTA TGAGCACAAG CACTCCAGCA
    ATCAGCAGCC CTAGTCCTAC CACAGCCTCC AAAGAAGGAG ACCTGGGTGG GAAACAGTGG
    CAAGATAGCT CAACCCAACC TGAGCTGAGC TCTCGCTATC TCATTTCCAC TGGAAGCACT
    TCCCAGCCCA TCCTCACTTC CCAATCCTTG AGCATTCAGC CAAGTGAGGA AAATGTTTCC
    AGTTCAGATA CTGGTCCTAC CTCGGAGGGA GGCCTTGTAG CTACAACAAC AAGTGGTTCT
    GGCTTGTCAT CTTCCCGCAA CCCTATCACT TGGCCTGTGA CTCCATTTTA CAATACCTTG
    ACCAAAGGTC CAGAAATGGA GATTCACAGT GGCTCAGGGG AAGAAAGAGA ACAGCCTGAG
    GACAAAGATG AAAGCAATCC TGTAATATGG ACCAAGATCA GAGTACCTGG AAATGACGCT
    CCAGTGGAAA GTACAGAAAT GCCACTTGCA CCTCCACTAA CACCAGATCT CAGCAGGGAG
    TCCTGGTGGC CACCCTTCAG CACAGTAATG GAAGGACTGC TCCCCAGCCA AAGGCCCACT
    ACTTCCGAAA CTGGGACACC CAGAGTTGAG GGGATGGTTA CTGAAAAGCC AGCCAACACT
    CTGCTCCCTC TGGGAGGAGA CCACCAGCCA GAACCCTCAG GAAAGACGGC AAACCGTAAC
    CACCTGAAAC TTCCAAACAA CATGAACCAA ACAAAAAGTT CTGAACCAGT CCTGACTGAG
    GAGGATGCAA CAAGTCTGAT TACTGAGGGC TTTTTGCTAA ATGCCTCCAA TTACAAGCAG
    CTCACAAACG GCCACGGCTC TGCACACTGG ATCGTCGGAA ACTGGAGCGA GTGCTCCACC
    ACATGTGGCC TGGGGGCCTA CTGGAGAAGG GTGGAGTGCA GCACCCAGAT GGATTCTGAC
    TGTGCGGCCA TCCAGAGACC TGACCCTGCA AAAAGATGCC ACCTCCGTCC CTGTGCTGGC
    TGGAAAGTGG GAAACTGGAG CAAGTGCTCC AGAAACTGCA GTGGGGGCTT CAAGATACGC
    GAGATTCAGT GCGTGGACAG CCGGGACCAC CGGAACCTGA GGCCATTTCA CTGCCAGTTC
    CTGGCCGGCA TTCCTCCCCC ATTGAGCATG AGCTGTAACC CGGAGCCCTG TGAGGCGTGG
    CAGGTGGAGC CTTGGAGCCA GTGCTCCAGG TCCTGTGGAG GTGGAGTTCA GGAGAGAGGA
    GTGTTCTGTC CAGGAGGCCT CTGTGATTGG ACAAAAAGAC CCACATCCAC CATGTCTTGC
    AATGAGCACC TGTGCTGTCA CTGGGCCACT GGGAACTGGG ACCTGTGTTC CACTTCCTGT
    GGAGGTGGCT TTCAGAAGAG GACTGTCCAA TGTGTGCCCT CAGAGGGCAA TAAAACTGAA
    GACCAAGACC AATGTCTATG TGATCACAAA CCCAGACCTC CAGAATTCAA AAAATGCAAC
    CAGCAGGCCT GCAAGAAAAG TGCCGATTTA CTTTGCACTA AGGACAAACT GTCAGCCAGT
    TTCTGCCAGA CACTGAAAGC CATGAAGAAA TGTTCTGTGC CCACCGTGAG GGCTGAGTGC
    TGCTTCTCGT GTCCCCAGAC ACACATCACA CACACCCAAA GGCAAAGAAG GCAACGGTTG
    CTCCAAAAGT CAAAAGAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96109
    Clone ID Related Accession (Same CDS sequence) XM_017009907.1
    Accession Version XM_017009907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACTCA ATGTGTGGTG TATGGCAGGC AAGTGCATCA CAGTGGGGAA GAAACCAGAG 
    AGCATTCCTG GAGGCTGGGG CCGCTGGTCA CCCTGGTCCC ACTGTTCCAG GACCTGTGGG
    GCTGGAGTCC AGAGCGCAGA GAGGCTCTGC AACAACCCCG AGCCAAAGTT TGGAGGGAAA
    TATTGCACTG GAGAAAGAAA ACGCTATCGC TTGTGCAACG TCCACCCCTG TCGCTCAGAG
    GCACCAACAT TTCGGCAGAT GCAGTGCAGT GAATTTGACA CTGTTCCCTA CAAGAATGAA
    CTCTACCACT GGTTTCCCAT TTTTAACCCA GCACATCCTT GTGAGCTCTA CTGCCGACCC
    ATAGATGGCC AGTTTTCTGA GAAAATGCTG GATGCTGTCA TTGATGGTAC CCCTTGCTTT
    GAAGGCGGCA ACAGCAGAAA TGTCTGTATT AATGGCATAT GTAAGATGGT TGGCTGTGAC
    TATGAGATCG ATTCCAATGC CACCGAGGAT CGCTGCGGTG TGTGCCTGGG AGATGGCTCT
    TCCTGCCAGA CTGTGAGAAA GATGTTTAAG CAGAAGGAAG GATCTGGTTA TGTTGACATT
    GGGCTCATTC CAAAAGGAGC AAGGGACATA AGAGTGATGG AAATTGAGGG AGCTGGAAAC
    TTCCTGGCCA TCAGGAGTGA AGATCCTGAA AAATATTACC TGAATGGAGG GTTTATTATC
    CAGTGGAACG GGAACTATAA GCTGGCAGGG ACTGTCTTTC AGTATGACAG GAAAGGAGAC
    CTGGAAAAGC TGATGGCCAC AGGTCCCACC AATGAGTCTG TGTGGATCCA GCTTCTATTC
    CAGGTGACTA ACCCTGGCAT CAAGTATGAG TACACAATCC AGAAAGATGG CCTTGACAAT
    GATGTTGAGC AGCAGATGTA CTTCTGGCAG TACGGCCACT GGACAGAGTG CAGTGTGACC
    TGCGGGACAG GTATCCGCCG CCAAACTGCC CATTGCATAA AGAAGGGCCG CGGGATGGTG
    AAAGCTACAT TCTGTGACCC AGAAACACAG CCCAATGGGA GACAGAAGAA GTGCCATGAA
    AAGGCTTGTC CACCCAGGTG GTGGGCAGGG GAGTGGGAAG CATGCTCGGC GACATGCGGG
    CCCCACGGGG AGAAGAAGCG AACCGTGCTG TGCATCCAGA CCATGGTCTC TGACGAGCAG
    GCTCTCCCGC CCACAGACTG CCAGCACCTG CTGAAGCCCA AGACCCTCCT TTCCTGCAAC
    AGAGACATCC TGTGCCCCTC GGACTGGACA GTGGGCAACT GGAGTGAGCT TCACTGGTTG
    GGTCCAAACA CAGTTCGACA CTCCTTCAGC TGTACATTCT CCTGTGCTCA CAGCATCTGC
    TTTTCAAAAG AGCTGATTTC ACAGAGCTGT TTTACATCAT GTTCTGTTTC CTGTGGTGGT
    GGAGTGCGGA TTCGCAGTGT CACATGTGCC AAGAACCATG ATGAACCTTG CGATGTGACA
    AGGAAACCCA ACAGCCGAGC TCTGTGTGGC CTCCAGCAAT GCCCTTCTAG CCGGAGAGTT
    CTGAAACCAA ACAAAGGCAC TATTTCCAAT GGAAAAAACC CACCAACACT AAAGCCCGTC
    CCTCCACCTA CATCCAGGCC CAGAATGCTG ACCACACCCA CAGGGCCTGA GTCTATGAGC
    ACAAGCACTC CAGCAATCAG CAGCCCTAGT CCTACCACAG CCTCCAAAGA AGGAGACCTG
    GGTGGGAAAC AGTGGCAAGA TAGCTCAACC CAACCTGAGC TGAGCTCTCG CTATCTCATT
    TCCACTGGAA GCACTTCCCA GCCCATCCTC ACTTCCCAAT CCTTGAGCAT TCAGCCAAGT
    GAGGAAAATG TTTCCAGTTC AGATACTGGT CCTACCTCGG AGGGAGGCCT TGTAGCTACA
    ACAACAAGTG GTTCTGGCTT GTCATCTTCC CGCAACCCTA TCACTTGGCC TGTGACTCCA
    TTTTACAATA CCTTGACCAA AGGTCCAGAA ATGGAGATTC ACAGTGGCTC AGGGGAAGAA
    AGAGAACAGC CTGAGGACAA AGATGAAAGC AATCCTGTAA TATGGACCAA GATCAGAGTA
    CCTGGAAATG ACGCTCCAGT GGAAAGTACA GAAATGCCAC TTGCACCTCC ACTAACACCA
    GATCTCAGCA GGGAGTCCTG GTGGCCACCC TTCAGCACAG TAATGGAAGG ACTGCTCCCC
    AGCCAAAGGC CCACTACTTC CGAAACTGGG ACACCCAGAG TTGAGGGGAT GGTTACTGAA
    AAGCCAGCCA ACACTCTGCT CCCTCTGGGA GGAGACCACC AGCCAGAACC CTCAGGAAAG
    ACGGCAAACC GTAACCACCT GAAACTTCCA AACAACATGA ACCAAACAAA AAGTTCTGAA
    CCAGTCCTGA CTGAGGAGGA TGCAACAAGT CTGATTACTG AGGGCTTTTT GCTAAATGCC
    TCCAATTACA AGCAGCTCAC AAACGGCCAC GGCTCTGCAC ACTGGATCGT CGGAAACTGG
    AGCGAGTGCT CCACCACATG TGGCCTGGGG GCCTACTGGA GAAGGGTGGA GTGCAGCACC
    CAGATGGATT CTGACTGTGC GGCCATCCAG AGACCTGACC CTGCAAAAAG ATGCCACCTC
    CGTCCCTGTG CTGGCTGGAA AGTGGGAAAC TGGAGCAAGT GCTCCAGAAA CTGCAGTGGG
    GGCTTCAAGA TACGCGAGAT TCAGTGCGTG GACAGCCGGG ACCACCGGAA CCTGAGGCCA
    TTTCACTGCC AGTTCCTGGC CGGCATTCCT CCCCCATTGA GCATGAGCTG TAACCCGGAG
    CCCTGTGAGG CGTGGCAGGT GGAGCCTTGG AGCCAGTGCT CCAGGTCCTG TGGAGGTGGA
    GTTCAGGAGA GAGGAGTGTT CTGTCCAGGA GGCCTCTGTG ATTGGACAAA AAGACCCACA
    TCCACCATGT CTTGCAATGA GCACCTGTGC TGTCACTGGG CCACTGGGAA CTGGGACCTG
    TGTTCCACTT CCTGTGGAGG TGGCTTTCAG AAGAGGACTG TCCAATGTGT GCCCTCAGAG
    GGCAATAAAA CTGAAGACCA AGACCAATGT CTATGTGATC ACAAACCCAG ACCTCCAGAA
    TTCAAAAAAT GCAACCAGCA GGCCTGCAAG AAAAGTGCCG ATTTACTTTG CACTAAGGAC
    AAACTGTCAG CCAGTTTCTG CCAGACACTG AAAGCCATGA AGAAATGTTC TGTGCCCACC
    GTGAGGGCTG AGTGCTGCTT CTCGTGTCCC CAGACACACA TCACACACAC CCAAAGGCAA
    AGAAGGCAAC GGTTGCTCCA AAAGTCAAAA GAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865396.1
    CDS175..3513
    Translation

    Target ORF information:

    RefSeq Version XM_017009907.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAACTCA ATGTGTGGTG TATGGCAGGC AAGTGCATCA CAGTGGGGAA GAAACCAGAG 
    AGCATTCCTG GAGGCTGGGG CCGCTGGTCA CCCTGGTCCC ACTGTTCCAG GACCTGTGGG
    GCTGGAGTCC AGAGCGCAGA GAGGCTCTGC AACAACCCCG AGCCAAAGTT TGGAGGGAAA
    TATTGCACTG GAGAAAGAAA ACGCTATCGC TTGTGCAACG TCCACCCCTG TCGCTCAGAG
    GCACCAACAT TTCGGCAGAT GCAGTGCAGT GAATTTGACA CTGTTCCCTA CAAGAATGAA
    CTCTACCACT GGTTTCCCAT TTTTAACCCA GCACATCCTT GTGAGCTCTA CTGCCGACCC
    ATAGATGGCC AGTTTTCTGA GAAAATGCTG GATGCTGTCA TTGATGGTAC CCCTTGCTTT
    GAAGGCGGCA ACAGCAGAAA TGTCTGTATT AATGGCATAT GTAAGATGGT TGGCTGTGAC
    TATGAGATCG ATTCCAATGC CACCGAGGAT CGCTGCGGTG TGTGCCTGGG AGATGGCTCT
    TCCTGCCAGA CTGTGAGAAA GATGTTTAAG CAGAAGGAAG GATCTGGTTA TGTTGACATT
    GGGCTCATTC CAAAAGGAGC AAGGGACATA AGAGTGATGG AAATTGAGGG AGCTGGAAAC
    TTCCTGGCCA TCAGGAGTGA AGATCCTGAA AAATATTACC TGAATGGAGG GTTTATTATC
    CAGTGGAACG GGAACTATAA GCTGGCAGGG ACTGTCTTTC AGTATGACAG GAAAGGAGAC
    CTGGAAAAGC TGATGGCCAC AGGTCCCACC AATGAGTCTG TGTGGATCCA GCTTCTATTC
    CAGGTGACTA ACCCTGGCAT CAAGTATGAG TACACAATCC AGAAAGATGG CCTTGACAAT
    GATGTTGAGC AGCAGATGTA CTTCTGGCAG TACGGCCACT GGACAGAGTG CAGTGTGACC
    TGCGGGACAG GTATCCGCCG CCAAACTGCC CATTGCATAA AGAAGGGCCG CGGGATGGTG
    AAAGCTACAT TCTGTGACCC AGAAACACAG CCCAATGGGA GACAGAAGAA GTGCCATGAA
    AAGGCTTGTC CACCCAGGTG GTGGGCAGGG GAGTGGGAAG CATGCTCGGC GACATGCGGG
    CCCCACGGGG AGAAGAAGCG AACCGTGCTG TGCATCCAGA CCATGGTCTC TGACGAGCAG
    GCTCTCCCGC CCACAGACTG CCAGCACCTG CTGAAGCCCA AGACCCTCCT TTCCTGCAAC
    AGAGACATCC TGTGCCCCTC GGACTGGACA GTGGGCAACT GGAGTGAGCT TCACTGGTTG
    GGTCCAAACA CAGTTCGACA CTCCTTCAGC TGTACATTCT CCTGTGCTCA CAGCATCTGC
    TTTTCAAAAG AGCTGATTTC ACAGAGCTGT TTTACATCAT GTTCTGTTTC CTGTGGTGGT
    GGAGTGCGGA TTCGCAGTGT CACATGTGCC AAGAACCATG ATGAACCTTG CGATGTGACA
    AGGAAACCCA ACAGCCGAGC TCTGTGTGGC CTCCAGCAAT GCCCTTCTAG CCGGAGAGTT
    CTGAAACCAA ACAAAGGCAC TATTTCCAAT GGAAAAAACC CACCAACACT AAAGCCCGTC
    CCTCCACCTA CATCCAGGCC CAGAATGCTG ACCACACCCA CAGGGCCTGA GTCTATGAGC
    ACAAGCACTC CAGCAATCAG CAGCCCTAGT CCTACCACAG CCTCCAAAGA AGGAGACCTG
    GGTGGGAAAC AGTGGCAAGA TAGCTCAACC CAACCTGAGC TGAGCTCTCG CTATCTCATT
    TCCACTGGAA GCACTTCCCA GCCCATCCTC ACTTCCCAAT CCTTGAGCAT TCAGCCAAGT
    GAGGAAAATG TTTCCAGTTC AGATACTGGT CCTACCTCGG AGGGAGGCCT TGTAGCTACA
    ACAACAAGTG GTTCTGGCTT GTCATCTTCC CGCAACCCTA TCACTTGGCC TGTGACTCCA
    TTTTACAATA CCTTGACCAA AGGTCCAGAA ATGGAGATTC ACAGTGGCTC AGGGGAAGAA
    AGAGAACAGC CTGAGGACAA AGATGAAAGC AATCCTGTAA TATGGACCAA GATCAGAGTA
    CCTGGAAATG ACGCTCCAGT GGAAAGTACA GAAATGCCAC TTGCACCTCC ACTAACACCA
    GATCTCAGCA GGGAGTCCTG GTGGCCACCC TTCAGCACAG TAATGGAAGG ACTGCTCCCC
    AGCCAAAGGC CCACTACTTC CGAAACTGGG ACACCCAGAG TTGAGGGGAT GGTTACTGAA
    AAGCCAGCCA ACACTCTGCT CCCTCTGGGA GGAGACCACC AGCCAGAACC CTCAGGAAAG
    ACGGCAAACC GTAACCACCT GAAACTTCCA AACAACATGA ACCAAACAAA AAGTTCTGAA
    CCAGTCCTGA CTGAGGAGGA TGCAACAAGT CTGATTACTG AGGGCTTTTT GCTAAATGCC
    TCCAATTACA AGCAGCTCAC AAACGGCCAC GGCTCTGCAC ACTGGATCGT CGGAAACTGG
    AGCGAGTGCT CCACCACATG TGGCCTGGGG GCCTACTGGA GAAGGGTGGA GTGCAGCACC
    CAGATGGATT CTGACTGTGC GGCCATCCAG AGACCTGACC CTGCAAAAAG ATGCCACCTC
    CGTCCCTGTG CTGGCTGGAA AGTGGGAAAC TGGAGCAAGT GCTCCAGAAA CTGCAGTGGG
    GGCTTCAAGA TACGCGAGAT TCAGTGCGTG GACAGCCGGG ACCACCGGAA CCTGAGGCCA
    TTTCACTGCC AGTTCCTGGC CGGCATTCCT CCCCCATTGA GCATGAGCTG TAACCCGGAG
    CCCTGTGAGG CGTGGCAGGT GGAGCCTTGG AGCCAGTGCT CCAGGTCCTG TGGAGGTGGA
    GTTCAGGAGA GAGGAGTGTT CTGTCCAGGA GGCCTCTGTG ATTGGACAAA AAGACCCACA
    TCCACCATGT CTTGCAATGA GCACCTGTGC TGTCACTGGG CCACTGGGAA CTGGGACCTG
    TGTTCCACTT CCTGTGGAGG TGGCTTTCAG AAGAGGACTG TCCAATGTGT GCCCTCAGAG
    GGCAATAAAA CTGAAGACCA AGACCAATGT CTATGTGATC ACAAACCCAG ACCTCCAGAA
    TTCAAAAAAT GCAACCAGCA GGCCTGCAAG AAAAGTGCCG ATTTACTTTG CACTAAGGAC
    AAACTGTCAG CCAGTTTCTG CCAGACACTG AAAGCCATGA AGAAATGTTC TGTGCCCACC
    GTGAGGGCTG AGTGCTGCTT CTCGTGTCCC CAGACACACA TCACACACAC CCAAAGGCAA
    AGAAGGCAAC GGTTGCTCCA AAAGTCAAAA GAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101976
    Clone ID Related Accession (Same CDS sequence) NM_001324512.1 , NM_001324512.2
    Accession Version NM_001324512.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ250725.1, AC139777.3, BG108002.1, BC131733.1, AC034232.8 and CA413137.1. Transcript Variant: This variant (3) encodes isoform 3. The encoded isoform (3) may undergo proteolytic processing. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC131733.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGGT TATGTTGACA TTGGGCTCAT TCCAAAAGGA
    GCAAGGGACA TAAGAGTGAT GGAAATTGAG GGAGCTGGAA ACTTCCTGGC CATCAGGAGT
    GAAGATCCTG AAAAATATTA CCTGAATGGA GGGTTTATTA TCCAGTGGAA CGGGAACTAT
    AAGCTGGCAG GGACTGTCTT TCAGTATGAC AGGAAAGGAG ACCTGGAAAA GCTGATGGCC
    ACAGGTCCCA CCAATGAGTC TGTGTGGATC CAGCTTCTAT TCCAGGTGAC TAACCCTGGC
    ATCAAGTATG AGTACACAAT CCAGAAAGAT GGCCTTGACA ATGATGTTGA GCAGCAGATG
    TACTTCTGGC AGTACGGCCA CTGGACAGAG TGCAGTGTGA CCTGCGGGAC AGGTATCCGC
    CGCCAAACTG CCCATTGCAT AAAGAAGGGC CGCGGGATGG TGAAAGCTAC ATTCTGTGAC
    CCAGAAACAC AGCCCAATGG GAGACAGAAG AAGTGCCATG AAAAGGCTTG TCCACCCAGG
    TGGTGGGCAG GGGAGTGGGA AGCATGCTCG GCGACATGCG GGCCCCACGG GGAGAAGAAG
    CGAACCGTGC TGTGCATCCA GACCATGGTC TCTGACGAGC AGGCTCTCCC GCCCACAGAC
    TGCCAGCACC TGCTGAAGCC CAAGACCCTC CTTTCCTGCA ACAGAGACAT CCTGTGCCCC
    TCGGACTGGA CAGTGGGCAA CTGGAGTGAG TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311441.1
    CDS337..4866
    Translation

    Target ORF information:

    RefSeq Version NM_001324512.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGGT TATGTTGACA TTGGGCTCAT TCCAAAAGGA
    GCAAGGGACA TAAGAGTGAT GGAAATTGAG GGAGCTGGAA ACTTCCTGGC CATCAGGAGT
    GAAGATCCTG AAAAATATTA CCTGAATGGA GGGTTTATTA TCCAGTGGAA CGGGAACTAT
    AAGCTGGCAG GGACTGTCTT TCAGTATGAC AGGAAAGGAG ACCTGGAAAA GCTGATGGCC
    ACAGGTCCCA CCAATGAGTC TGTGTGGATC CAGCTTCTAT TCCAGGTGAC TAACCCTGGC
    ATCAAGTATG AGTACACAAT CCAGAAAGAT GGCCTTGACA ATGATGTTGA GCAGCAGATG
    TACTTCTGGC AGTACGGCCA CTGGACAGAG TGCAGTGTGA CCTGCGGGAC AGGTATCCGC
    CGCCAAACTG CCCATTGCAT AAAGAAGGGC CGCGGGATGG TGAAAGCTAC ATTCTGTGAC
    CCAGAAACAC AGCCCAATGG GAGACAGAAG AAGTGCCATG AAAAGGCTTG TCCACCCAGG
    TGGTGGGCAG GGGAGTGGGA AGCATGCTCG GCGACATGCG GGCCCCACGG GGAGAAGAAG
    CGAACCGTGC TGTGCATCCA GACCATGGTC TCTGACGAGC AGGCTCTCCC GCCCACAGAC
    TGCCAGCACC TGCTGAAGCC CAAGACCCTC CTTTCCTGCA ACAGAGACAT CCTGTGCCCC
    TCGGACTGGA CAGTGGGCAA CTGGAGTGAG TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu101976
    Clone ID Related Accession (Same CDS sequence) NM_001324512.1 , NM_001324512.2
    Accession Version NM_001324512.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-27
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG108002.1, BC131733.1, AC034232.8 and CA413137.1. On May 31, 2019 this sequence version replaced NM_001324512.1. Transcript Variant: This variant (3) encodes isoform 3. The encoded isoform (3) may undergo proteolytic processing. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC131733.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGGT TATGTTGACA TTGGGCTCAT TCCAAAAGGA
    GCAAGGGACA TAAGAGTGAT GGAAATTGAG GGAGCTGGAA ACTTCCTGGC CATCAGGAGT
    GAAGATCCTG AAAAATATTA CCTGAATGGA GGGTTTATTA TCCAGTGGAA CGGGAACTAT
    AAGCTGGCAG GGACTGTCTT TCAGTATGAC AGGAAAGGAG ACCTGGAAAA GCTGATGGCC
    ACAGGTCCCA CCAATGAGTC TGTGTGGATC CAGCTTCTAT TCCAGGTGAC TAACCCTGGC
    ATCAAGTATG AGTACACAAT CCAGAAAGAT GGCCTTGACA ATGATGTTGA GCAGCAGATG
    TACTTCTGGC AGTACGGCCA CTGGACAGAG TGCAGTGTGA CCTGCGGGAC AGGTATCCGC
    CGCCAAACTG CCCATTGCAT AAAGAAGGGC CGCGGGATGG TGAAAGCTAC ATTCTGTGAC
    CCAGAAACAC AGCCCAATGG GAGACAGAAG AAGTGCCATG AAAAGGCTTG TCCACCCAGG
    TGGTGGGCAG GGGAGTGGGA AGCATGCTCG GCGACATGCG GGCCCCACGG GGAGAAGAAG
    CGAACCGTGC TGTGCATCCA GACCATGGTC TCTGACGAGC AGGCTCTCCC GCCCACAGAC
    TGCCAGCACC TGCTGAAGCC CAAGACCCTC CTTTCCTGCA ACAGAGACAT CCTGTGCCCC
    TCGGACTGGA CAGTGGGCAA CTGGAGTGAG TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001311441.1
    CDS135..4664
    Translation

    Target ORF information:

    RefSeq Version NM_001324512.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001324512.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGGT TATGTTGACA TTGGGCTCAT TCCAAAAGGA
    GCAAGGGACA TAAGAGTGAT GGAAATTGAG GGAGCTGGAA ACTTCCTGGC CATCAGGAGT
    GAAGATCCTG AAAAATATTA CCTGAATGGA GGGTTTATTA TCCAGTGGAA CGGGAACTAT
    AAGCTGGCAG GGACTGTCTT TCAGTATGAC AGGAAAGGAG ACCTGGAAAA GCTGATGGCC
    ACAGGTCCCA CCAATGAGTC TGTGTGGATC CAGCTTCTAT TCCAGGTGAC TAACCCTGGC
    ATCAAGTATG AGTACACAAT CCAGAAAGAT GGCCTTGACA ATGATGTTGA GCAGCAGATG
    TACTTCTGGC AGTACGGCCA CTGGACAGAG TGCAGTGTGA CCTGCGGGAC AGGTATCCGC
    CGCCAAACTG CCCATTGCAT AAAGAAGGGC CGCGGGATGG TGAAAGCTAC ATTCTGTGAC
    CCAGAAACAC AGCCCAATGG GAGACAGAAG AAGTGCCATG AAAAGGCTTG TCCACCCAGG
    TGGTGGGCAG GGGAGTGGGA AGCATGCTCG GCGACATGCG GGCCCCACGG GGAGAAGAAG
    CGAACCGTGC TGTGCATCCA GACCATGGTC TCTGACGAGC AGGCTCTCCC GCCCACAGAC
    TGCCAGCACC TGCTGAAGCC CAAGACCCTC CTTTCCTGCA ACAGAGACAT CCTGTGCCCC
    TCGGACTGGA CAGTGGGCAA CTGGAGTGAG TGTTCTGTTT CCTGTGGTGG TGGAGTGCGG
    ATTCGCAGTG TCACATGTGC CAAGAACCAT GATGAACCTT GCGATGTGAC AAGGAAACCC
    AACAGCCGAG CTCTGTGTGG CCTCCAGCAA TGCCCTTCTA GCCGGAGAGT TCTGAAACCA
    AACAAAGGCA CTATTTCCAA TGGAAAAAAC CCACCAACAC TAAAGCCCGT CCCTCCACCT
    ACATCCAGGC CCAGAATGCT GACCACACCC ACAGGGCCTG AGTCTATGAG CACAAGCACT
    CCAGCAATCA GCAGCCCTAG TCCTACCACA GCCTCCAAAG AAGGAGACCT GGGTGGGAAA
    CAGTGGCAAG ATAGCTCAAC CCAACCTGAG CTGAGCTCTC GCTATCTCAT TTCCACTGGA
    AGCACTTCCC AGCCCATCCT CACTTCCCAA TCCTTGAGCA TTCAGCCAAG TGAGGAAAAT
    GTTTCCAGTT CAGATACTGG TCCTACCTCG GAGGGAGGCC TTGTAGCTAC AACAACAAGT
    GGTTCTGGCT TGTCATCTTC CCGCAACCCT ATCACTTGGC CTGTGACTCC ATTTTACAAT
    ACCTTGACCA AAGGTCCAGA AATGGAGATT CACAGTGGCT CAGGGGAAGA AAGAGAACAG
    CCTGAGGACA AAGATGAAAG CAATCCTGTA ATATGGACCA AGATCAGAGT ACCTGGAAAT
    GACGCTCCAG TGGAAAGTAC AGAAATGCCA CTTGCACCTC CACTAACACC AGATCTCAGC
    AGGGAGTCCT GGTGGCCACC CTTCAGCACA GTAATGGAAG GACTGCTCCC CAGCCAAAGG
    CCCACTACTT CCGAAACTGG GACACCCAGA GTTGAGGGGA TGGTTACTGA AAAGCCAGCC
    AACACTCTGC TCCCTCTGGG AGGAGACCAC CAGCCAGAAC CCTCAGGAAA GACGGCAAAC
    CGTAACCACC TGAAACTTCC AAACAACATG AACCAAACAA AAAGTTCTGA ACCAGTCCTG
    ACTGAGGAGG ATGCAACAAG TCTGATTACT GAGGGCTTTT TGCTAAATGC CTCCAATTAC
    AAGCAGCTCA CAAACGGCCA CGGCTCTGCA CACTGGATCG TCGGAAACTG GAGCGAGTGC
    TCCACCACAT GTGGCCTGGG GGCCTACTGG AGAAGGGTGG AGTGCAGCAC CCAGATGGAT
    TCTGACTGTG CGGCCATCCA GAGACCTGAC CCTGCAAAAA GATGCCACCT CCGTCCCTGT
    GCTGGCTGGA AAGTGGGAAA CTGGAGCAAG TGCTCCAGAA ACTGCAGTGG GGGCTTCAAG
    ATACGCGAGA TTCAGTGCGT GGACAGCCGG GACCACCGGA ACCTGAGGCC ATTTCACTGC
    CAGTTCCTGG CCGGCATTCC TCCCCCATTG AGCATGAGCT GTAACCCGGA GCCCTGTGAG
    GCGTGGCAGG TGGAGCCTTG GAGCCAGTGC TCCAGGTCCT GTGGAGGTGG AGTTCAGGAG
    AGAGGAGTGT TCTGTCCAGG AGGCCTCTGT GATTGGACAA AAAGACCCAC ATCCACCATG
    TCTTGCAATG AGCACCTGTG CTGTCACTGG GCCACTGGGA ACTGGGACCT GTGTTCCACT
    TCCTGTGGAG GTGGCTTTCA GAAGAGGACT GTCCAATGTG TGCCCTCAGA GGGCAATAAA
    ACTGAAGACC AAGACCAATG TCTATGTGAT CACAAACCCA GACCTCCAGA ATTCAAAAAA
    TGCAACCAGC AGGCCTGCAA GAAAAGTGCC GATTTACTTT GCACTAAGGA CAAACTGTCA
    GCCAGTTTCT GCCAGACACT GAAAGCCATG AAGAAATGTT CTGTGCCCAC CGTGAGGGCT
    GAGTGCTGCT TCTCGTGTCC CCAGACACAC ATCACACACA CCCAAAGGCA AAGAAGGCAA
    CGGTTGCTCC AAAAGTCAAA AGAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96107
    Clone ID Related Accession (Same CDS sequence) XM_017009905.1
    Accession Version XM_017009905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGCTTCA CTGGTTGGGT
    CCAAACACAG TTCGACACTC CTTCAGCTGT ACATTCTCCT GTGCTCACAG CATCTGCTTT
    TCAAAAGAGC TGATTTCACA GAGCTGTTTT ACATCATGTT CTGTTTCCTG TGGTGGTGGA
    GTGCGGATTC GCAGTGTCAC ATGTGCCAAG AACCATGATG AACCTTGCGA TGTGACAAGG
    AAACCCAACA GCCGAGCTCT GTGTGGCCTC CAGCAATGCC CTTCTAGCCG GAGAGTTCTG
    AAACCAAACA AAGGCACTAT TTCCAATGGA AAAAACCCAC CAACACTAAA GCCCGTCCCT
    CCACCTACAT CCAGGCCCAG AATGCTGACC ACACCCACAG GGCCTGAGTC TATGAGCACA
    AGCACTCCAG CAATCAGCAG CCCTAGTCCT ACCACAGCCT CCAAAGAAGG AGACCTGGGT
    GGGAAACAGT GGCAAGATAG CTCAACCCAA CCTGAGCTGA GCTCTCGCTA TCTCATTTCC
    ACTGGAAGCA CTTCCCAGCC CATCCTCACT TCCCAATCCT TGAGCATTCA GCCAAGTGAG
    GAAAATGTTT CCAGTTCAGA TACTGGTCCT ACCTCGGAGG GAGGCCTTGT AGCTACAACA
    ACAAGTGGTT CTGGCTTGTC ATCTTCCCGC AACCCTATCA CTTGGCCTGT GACTCCATTT
    TACAATACCT TGACCAAAGG TCCAGAAATG GAGATTCACA GTGGCTCAGG GGAAGAAAGA
    GAACAGCCTG AGGACAAAGA TGAAAGCAAT CCTGTAATAT GGACCAAGAT CAGAGTACCT
    GGAAATGACG CTCCAGTGGA AAGTACAGAA ATGCCACTTG CACCTCCACT AACACCAGAT
    CTCAGCAGGG AGTCCTGGTG GCCACCCTTC AGCACAGTAA TGGAAGGACT GCTCCCCAGC
    CAAAGGCCCA CTACTTCCGA AACTGGGACA CCCAGAGTTG AGGGGATGGT TACTGAAAAG
    CCAGCCAACA CTCTGCTCCC TCTGGGAGGA GACCACCAGC CAGAACCCTC AGGAAAGACG
    GCAAACCGTA ACCACCTGAA ACTTCCAAAC AACATGAACC AAACAAAAAG TTCTGAACCA
    GTCCTGACTG AGGAGGATGC AACAAGTCTG ATTACTGAGG GCTTTTTGCT AAATGCCTCC
    AATTACAAGC AGCTCACAAA CGGCCACGGC TCTGCACACT GGATCGTCGG AAACTGGAGC
    GAGTGCTCCA CCACATGTGG CCTGGGGGCC TACTGGAGAA GGGTGGAGTG CAGCACCCAG
    ATGGATTCTG ACTGTGCGGC CATCCAGAGA CCTGACCCTG CAAAAAGATG CCACCTCCGT
    CCCTGTGCTG GCTGGAAAGT GGGAAACTGG AGCAAGTGCT CCAGAAACTG CAGTGGGGGC
    TTCAAGATAC GCGAGATTCA GTGCGTGGAC AGCCGGGACC ACCGGAACCT GAGGCCATTT
    CACTGCCAGT TCCTGGCCGG CATTCCTCCC CCATTGAGCA TGAGCTGTAA CCCGGAGCCC
    TGTGAGGCGT GGCAGGTGGA GCCTTGGAGC CAGTGCTCCA GGTCCTGTGG AGGTGGAGTT
    CAGGAGAGAG GAGTGTTCTG TCCAGGAGGC CTCTGTGATT GGACAAAAAG ACCCACATCC
    ACCATGTCTT GCAATGAGCA CCTGTGCTGT CACTGGGCCA CTGGGAACTG GGACCTGTGT
    TCCACTTCCT GTGGAGGTGG CTTTCAGAAG AGGACTGTCC AATGTGTGCC CTCAGAGGGC
    AATAAAACTG AAGACCAAGA CCAATGTCTA TGTGATCACA AACCCAGACC TCCAGAATTC
    AAAAAATGCA ACCAGCAGGC CTGCAAGAAA AGTGCCGATT TACTTTGCAC TAAGGACAAA
    CTGTCAGCCA GTTTCTGCCA GACACTGAAA GCCATGAAGA AATGTTCTGT GCCCACCGTG
    AGGGCTGAGT GCTGCTTCTC GTGTCCCCAG ACACACATCA CACACACCCA AAGGCAAAGA
    AGGCAACGGT TGCTCCAAAA GTCAAAAGAA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865394.1
    CDS325..5220
    Translation

    Target ORF information:

    RefSeq Version XM_017009905.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGCTTCA CTGGTTGGGT
    CCAAACACAG TTCGACACTC CTTCAGCTGT ACATTCTCCT GTGCTCACAG CATCTGCTTT
    TCAAAAGAGC TGATTTCACA GAGCTGTTTT ACATCATGTT CTGTTTCCTG TGGTGGTGGA
    GTGCGGATTC GCAGTGTCAC ATGTGCCAAG AACCATGATG AACCTTGCGA TGTGACAAGG
    AAACCCAACA GCCGAGCTCT GTGTGGCCTC CAGCAATGCC CTTCTAGCCG GAGAGTTCTG
    AAACCAAACA AAGGCACTAT TTCCAATGGA AAAAACCCAC CAACACTAAA GCCCGTCCCT
    CCACCTACAT CCAGGCCCAG AATGCTGACC ACACCCACAG GGCCTGAGTC TATGAGCACA
    AGCACTCCAG CAATCAGCAG CCCTAGTCCT ACCACAGCCT CCAAAGAAGG AGACCTGGGT
    GGGAAACAGT GGCAAGATAG CTCAACCCAA CCTGAGCTGA GCTCTCGCTA TCTCATTTCC
    ACTGGAAGCA CTTCCCAGCC CATCCTCACT TCCCAATCCT TGAGCATTCA GCCAAGTGAG
    GAAAATGTTT CCAGTTCAGA TACTGGTCCT ACCTCGGAGG GAGGCCTTGT AGCTACAACA
    ACAAGTGGTT CTGGCTTGTC ATCTTCCCGC AACCCTATCA CTTGGCCTGT GACTCCATTT
    TACAATACCT TGACCAAAGG TCCAGAAATG GAGATTCACA GTGGCTCAGG GGAAGAAAGA
    GAACAGCCTG AGGACAAAGA TGAAAGCAAT CCTGTAATAT GGACCAAGAT CAGAGTACCT
    GGAAATGACG CTCCAGTGGA AAGTACAGAA ATGCCACTTG CACCTCCACT AACACCAGAT
    CTCAGCAGGG AGTCCTGGTG GCCACCCTTC AGCACAGTAA TGGAAGGACT GCTCCCCAGC
    CAAAGGCCCA CTACTTCCGA AACTGGGACA CCCAGAGTTG AGGGGATGGT TACTGAAAAG
    CCAGCCAACA CTCTGCTCCC TCTGGGAGGA GACCACCAGC CAGAACCCTC AGGAAAGACG
    GCAAACCGTA ACCACCTGAA ACTTCCAAAC AACATGAACC AAACAAAAAG TTCTGAACCA
    GTCCTGACTG AGGAGGATGC AACAAGTCTG ATTACTGAGG GCTTTTTGCT AAATGCCTCC
    AATTACAAGC AGCTCACAAA CGGCCACGGC TCTGCACACT GGATCGTCGG AAACTGGAGC
    GAGTGCTCCA CCACATGTGG CCTGGGGGCC TACTGGAGAA GGGTGGAGTG CAGCACCCAG
    ATGGATTCTG ACTGTGCGGC CATCCAGAGA CCTGACCCTG CAAAAAGATG CCACCTCCGT
    CCCTGTGCTG GCTGGAAAGT GGGAAACTGG AGCAAGTGCT CCAGAAACTG CAGTGGGGGC
    TTCAAGATAC GCGAGATTCA GTGCGTGGAC AGCCGGGACC ACCGGAACCT GAGGCCATTT
    CACTGCCAGT TCCTGGCCGG CATTCCTCCC CCATTGAGCA TGAGCTGTAA CCCGGAGCCC
    TGTGAGGCGT GGCAGGTGGA GCCTTGGAGC CAGTGCTCCA GGTCCTGTGG AGGTGGAGTT
    CAGGAGAGAG GAGTGTTCTG TCCAGGAGGC CTCTGTGATT GGACAAAAAG ACCCACATCC
    ACCATGTCTT GCAATGAGCA CCTGTGCTGT CACTGGGCCA CTGGGAACTG GGACCTGTGT
    TCCACTTCCT GTGGAGGTGG CTTTCAGAAG AGGACTGTCC AATGTGTGCC CTCAGAGGGC
    AATAAAACTG AAGACCAAGA CCAATGTCTA TGTGATCACA AACCCAGACC TCCAGAATTC
    AAAAAATGCA ACCAGCAGGC CTGCAAGAAA AGTGCCGATT TACTTTGCAC TAAGGACAAA
    CTGTCAGCCA GTTTCTGCCA GACACTGAAA GCCATGAAGA AATGTTCTGT GCCCACCGTG
    AGGGCTGAGT GCTGCTTCTC GTGTCCCCAG ACACACATCA CACACACCCA AAGGCAAAGA
    AGGCAACGGT TGCTCCAAAA GTCAAAAGAA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29902
    Clone ID Related Accession (Same CDS sequence) NM_030955.4 , NM_030955.3
    Accession Version NM_030955.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058841.1, AY358745.1, AJ250725.1, AW938686.1, AC034232.8 and CA413137.1. On Nov 23, 2018 this sequence version replaced NM_030955.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ250725.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000504830.6/ ENSP00000422554.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGTGTTC TGTTTCCTGT
    GGTGGTGGAG TGCGGATTCG CAGTGTCACA TGTGCCAAGA ACCATGATGA ACCTTGCGAT
    GTGACAAGGA AACCCAACAG CCGAGCTCTG TGTGGCCTCC AGCAATGCCC TTCTAGCCGG
    AGAGTTCTGA AACCAAACAA AGGCACTATT TCCAATGGAA AAAACCCACC AACACTAAAG
    CCCGTCCCTC CACCTACATC CAGGCCCAGA ATGCTGACCA CACCCACAGG GCCTGAGTCT
    ATGAGCACAA GCACTCCAGC AATCAGCAGC CCTAGTCCTA CCACAGCCTC CAAAGAAGGA
    GACCTGGGTG GGAAACAGTG GCAAGATAGC TCAACCCAAC CTGAGCTGAG CTCTCGCTAT
    CTCATTTCCA CTGGAAGCAC TTCCCAGCCC ATCCTCACTT CCCAATCCTT GAGCATTCAG
    CCAAGTGAGG AAAATGTTTC CAGTTCAGAT ACTGGTCCTA CCTCGGAGGG AGGCCTTGTA
    GCTACAACAA CAAGTGGTTC TGGCTTGTCA TCTTCCCGCA ACCCTATCAC TTGGCCTGTG
    ACTCCATTTT ACAATACCTT GACCAAAGGT CCAGAAATGG AGATTCACAG TGGCTCAGGG
    GAAGAAAGAG AACAGCCTGA GGACAAAGAT GAAAGCAATC CTGTAATATG GACCAAGATC
    AGAGTACCTG GAAATGACGC TCCAGTGGAA AGTACAGAAA TGCCACTTGC ACCTCCACTA
    ACACCAGATC TCAGCAGGGA GTCCTGGTGG CCACCCTTCA GCACAGTAAT GGAAGGACTG
    CTCCCCAGCC AAAGGCCCAC TACTTCCGAA ACTGGGACAC CCAGAGTTGA GGGGATGGTT
    ACTGAAAAGC CAGCCAACAC TCTGCTCCCT CTGGGAGGAG ACCACCAGCC AGAACCCTCA
    GGAAAGACGG CAAACCGTAA CCACCTGAAA CTTCCAAACA ACATGAACCA AACAAAAAGT
    TCTGAACCAG TCCTGACTGA GGAGGATGCA ACAAGTCTGA TTACTGAGGG CTTTTTGCTA
    AATGCCTCCA ATTACAAGCA GCTCACAAAC GGCCACGGCT CTGCACACTG GATCGTCGGA
    AACTGGAGCG AGTGCTCCAC CACATGTGGC CTGGGGGCCT ACTGGAGAAG GGTGGAGTGC
    AGCACCCAGA TGGATTCTGA CTGTGCGGCC ATCCAGAGAC CTGACCCTGC AAAAAGATGC
    CACCTCCGTC CCTGTGCTGG CTGGAAAGTG GGAAACTGGA GCAAGTGCTC CAGAAACTGC
    AGTGGGGGCT TCAAGATACG CGAGATTCAG TGCGTGGACA GCCGGGACCA CCGGAACCTG
    AGGCCATTTC ACTGCCAGTT CCTGGCCGGC ATTCCTCCCC CATTGAGCAT GAGCTGTAAC
    CCGGAGCCCT GTGAGGCGTG GCAGGTGGAG CCTTGGAGCC AGTGCTCCAG GTCCTGTGGA
    GGTGGAGTTC AGGAGAGAGG AGTGTTCTGT CCAGGAGGCC TCTGTGATTG GACAAAAAGA
    CCCACATCCA CCATGTCTTG CAATGAGCAC CTGTGCTGTC ACTGGGCCAC TGGGAACTGG
    GACCTGTGTT CCACTTCCTG TGGAGGTGGC TTTCAGAAGA GGACTGTCCA ATGTGTGCCC
    TCAGAGGGCA ATAAAACTGA AGACCAAGAC CAATGTCTAT GTGATCACAA ACCCAGACCT
    CCAGAATTCA AAAAATGCAA CCAGCAGGCC TGCAAGAAAA GTGCCGATTT ACTTTGCACT
    AAGGACAAAC TGTCAGCCAG TTTCTGCCAG ACACTGAAAG CCATGAAGAA ATGTTCTGTG
    CCCACCGTGA GGGCTGAGTG CTGCTTCTCG TGTCCCCAGA CACACATCAC ACACACCCAA
    AGGCAAAGAA GGCAACGGTT GCTCCAAAAG TCAAAAGAAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_112217.2
    CDS135..4919
    Translation

    Target ORF information:

    RefSeq Version NM_030955.4
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_030955.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGTGTTC TGTTTCCTGT
    GGTGGTGGAG TGCGGATTCG CAGTGTCACA TGTGCCAAGA ACCATGATGA ACCTTGCGAT
    GTGACAAGGA AACCCAACAG CCGAGCTCTG TGTGGCCTCC AGCAATGCCC TTCTAGCCGG
    AGAGTTCTGA AACCAAACAA AGGCACTATT TCCAATGGAA AAAACCCACC AACACTAAAG
    CCCGTCCCTC CACCTACATC CAGGCCCAGA ATGCTGACCA CACCCACAGG GCCTGAGTCT
    ATGAGCACAA GCACTCCAGC AATCAGCAGC CCTAGTCCTA CCACAGCCTC CAAAGAAGGA
    GACCTGGGTG GGAAACAGTG GCAAGATAGC TCAACCCAAC CTGAGCTGAG CTCTCGCTAT
    CTCATTTCCA CTGGAAGCAC TTCCCAGCCC ATCCTCACTT CCCAATCCTT GAGCATTCAG
    CCAAGTGAGG AAAATGTTTC CAGTTCAGAT ACTGGTCCTA CCTCGGAGGG AGGCCTTGTA
    GCTACAACAA CAAGTGGTTC TGGCTTGTCA TCTTCCCGCA ACCCTATCAC TTGGCCTGTG
    ACTCCATTTT ACAATACCTT GACCAAAGGT CCAGAAATGG AGATTCACAG TGGCTCAGGG
    GAAGAAAGAG AACAGCCTGA GGACAAAGAT GAAAGCAATC CTGTAATATG GACCAAGATC
    AGAGTACCTG GAAATGACGC TCCAGTGGAA AGTACAGAAA TGCCACTTGC ACCTCCACTA
    ACACCAGATC TCAGCAGGGA GTCCTGGTGG CCACCCTTCA GCACAGTAAT GGAAGGACTG
    CTCCCCAGCC AAAGGCCCAC TACTTCCGAA ACTGGGACAC CCAGAGTTGA GGGGATGGTT
    ACTGAAAAGC CAGCCAACAC TCTGCTCCCT CTGGGAGGAG ACCACCAGCC AGAACCCTCA
    GGAAAGACGG CAAACCGTAA CCACCTGAAA CTTCCAAACA ACATGAACCA AACAAAAAGT
    TCTGAACCAG TCCTGACTGA GGAGGATGCA ACAAGTCTGA TTACTGAGGG CTTTTTGCTA
    AATGCCTCCA ATTACAAGCA GCTCACAAAC GGCCACGGCT CTGCACACTG GATCGTCGGA
    AACTGGAGCG AGTGCTCCAC CACATGTGGC CTGGGGGCCT ACTGGAGAAG GGTGGAGTGC
    AGCACCCAGA TGGATTCTGA CTGTGCGGCC ATCCAGAGAC CTGACCCTGC AAAAAGATGC
    CACCTCCGTC CCTGTGCTGG CTGGAAAGTG GGAAACTGGA GCAAGTGCTC CAGAAACTGC
    AGTGGGGGCT TCAAGATACG CGAGATTCAG TGCGTGGACA GCCGGGACCA CCGGAACCTG
    AGGCCATTTC ACTGCCAGTT CCTGGCCGGC ATTCCTCCCC CATTGAGCAT GAGCTGTAAC
    CCGGAGCCCT GTGAGGCGTG GCAGGTGGAG CCTTGGAGCC AGTGCTCCAG GTCCTGTGGA
    GGTGGAGTTC AGGAGAGAGG AGTGTTCTGT CCAGGAGGCC TCTGTGATTG GACAAAAAGA
    CCCACATCCA CCATGTCTTG CAATGAGCAC CTGTGCTGTC ACTGGGCCAC TGGGAACTGG
    GACCTGTGTT CCACTTCCTG TGGAGGTGGC TTTCAGAAGA GGACTGTCCA ATGTGTGCCC
    TCAGAGGGCA ATAAAACTGA AGACCAAGAC CAATGTCTAT GTGATCACAA ACCCAGACCT
    CCAGAATTCA AAAAATGCAA CCAGCAGGCC TGCAAGAAAA GTGCCGATTT ACTTTGCACT
    AAGGACAAAC TGTCAGCCAG TTTCTGCCAG ACACTGAAAG CCATGAAGAA ATGTTCTGTG
    CCCACCGTGA GGGCTGAGTG CTGCTTCTCG TGTCCCCAGA CACACATCAC ACACACCCAA
    AGGCAAAGAA GGCAACGGTT GCTCCAAAAG TCAAAAGAAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29902
    Clone ID Related Accession (Same CDS sequence) NM_030955.4 , NM_030955.3
    Accession Version NM_030955.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-03
    Organism Homo sapiens(human)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 12 isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ250725.1, AC139777.3, BG108002.1, BC058841.1, AY358745.1, AW938686.1, AC034232.8 and CA413137.1. On May 12, 2016 this sequence version replaced NM_030955.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ250725.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGTGTTC TGTTTCCTGT
    GGTGGTGGAG TGCGGATTCG CAGTGTCACA TGTGCCAAGA ACCATGATGA ACCTTGCGAT
    GTGACAAGGA AACCCAACAG CCGAGCTCTG TGTGGCCTCC AGCAATGCCC TTCTAGCCGG
    AGAGTTCTGA AACCAAACAA AGGCACTATT TCCAATGGAA AAAACCCACC AACACTAAAG
    CCCGTCCCTC CACCTACATC CAGGCCCAGA ATGCTGACCA CACCCACAGG GCCTGAGTCT
    ATGAGCACAA GCACTCCAGC AATCAGCAGC CCTAGTCCTA CCACAGCCTC CAAAGAAGGA
    GACCTGGGTG GGAAACAGTG GCAAGATAGC TCAACCCAAC CTGAGCTGAG CTCTCGCTAT
    CTCATTTCCA CTGGAAGCAC TTCCCAGCCC ATCCTCACTT CCCAATCCTT GAGCATTCAG
    CCAAGTGAGG AAAATGTTTC CAGTTCAGAT ACTGGTCCTA CCTCGGAGGG AGGCCTTGTA
    GCTACAACAA CAAGTGGTTC TGGCTTGTCA TCTTCCCGCA ACCCTATCAC TTGGCCTGTG
    ACTCCATTTT ACAATACCTT GACCAAAGGT CCAGAAATGG AGATTCACAG TGGCTCAGGG
    GAAGAAAGAG AACAGCCTGA GGACAAAGAT GAAAGCAATC CTGTAATATG GACCAAGATC
    AGAGTACCTG GAAATGACGC TCCAGTGGAA AGTACAGAAA TGCCACTTGC ACCTCCACTA
    ACACCAGATC TCAGCAGGGA GTCCTGGTGG CCACCCTTCA GCACAGTAAT GGAAGGACTG
    CTCCCCAGCC AAAGGCCCAC TACTTCCGAA ACTGGGACAC CCAGAGTTGA GGGGATGGTT
    ACTGAAAAGC CAGCCAACAC TCTGCTCCCT CTGGGAGGAG ACCACCAGCC AGAACCCTCA
    GGAAAGACGG CAAACCGTAA CCACCTGAAA CTTCCAAACA ACATGAACCA AACAAAAAGT
    TCTGAACCAG TCCTGACTGA GGAGGATGCA ACAAGTCTGA TTACTGAGGG CTTTTTGCTA
    AATGCCTCCA ATTACAAGCA GCTCACAAAC GGCCACGGCT CTGCACACTG GATCGTCGGA
    AACTGGAGCG AGTGCTCCAC CACATGTGGC CTGGGGGCCT ACTGGAGAAG GGTGGAGTGC
    AGCACCCAGA TGGATTCTGA CTGTGCGGCC ATCCAGAGAC CTGACCCTGC AAAAAGATGC
    CACCTCCGTC CCTGTGCTGG CTGGAAAGTG GGAAACTGGA GCAAGTGCTC CAGAAACTGC
    AGTGGGGGCT TCAAGATACG CGAGATTCAG TGCGTGGACA GCCGGGACCA CCGGAACCTG
    AGGCCATTTC ACTGCCAGTT CCTGGCCGGC ATTCCTCCCC CATTGAGCAT GAGCTGTAAC
    CCGGAGCCCT GTGAGGCGTG GCAGGTGGAG CCTTGGAGCC AGTGCTCCAG GTCCTGTGGA
    GGTGGAGTTC AGGAGAGAGG AGTGTTCTGT CCAGGAGGCC TCTGTGATTG GACAAAAAGA
    CCCACATCCA CCATGTCTTG CAATGAGCAC CTGTGCTGTC ACTGGGCCAC TGGGAACTGG
    GACCTGTGTT CCACTTCCTG TGGAGGTGGC TTTCAGAAGA GGACTGTCCA ATGTGTGCCC
    TCAGAGGGCA ATAAAACTGA AGACCAAGAC CAATGTCTAT GTGATCACAA ACCCAGACCT
    CCAGAATTCA AAAAATGCAA CCAGCAGGCC TGCAAGAAAA GTGCCGATTT ACTTTGCACT
    AAGGACAAAC TGTCAGCCAG TTTCTGCCAG ACACTGAAAG CCATGAAGAA ATGTTCTGTG
    CCCACCGTGA GGGCTGAGTG CTGCTTCTCG TGTCCCCAGA CACACATCAC ACACACCCAA
    AGGCAAAGAA GGCAACGGTT GCTCCAAAAG TCAAAAGAAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_112217.2
    CDS337..5121
    Translation

    Target ORF information:

    RefSeq Version NM_030955.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 12 (ADAMTS12), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_030955.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCCATGTG CCCAGAGGAG CTGGCTTGCA AACCTTTCCG TGGTGGCTCA GCTCCTTAAC 
    TTTGGGGCGC TTTGCTATGG GAGACAGCCT CAGCCAGGCC CGGTTCGCTT CCCGGACAGG
    AGGCAAGAGC ATTTTATCAA GGGCCTGCCA GAATACCACG TGGTGGGTCC AGTCCGAGTA
    GATGCCAGTG GGCATTTTTT GTCATATGGC TTGCACTATC CCATCACGAG CAGCAGGAGG
    AAGAGAGATT TGGATGGCTC AGAGGACTGG GTGTACTACA GAATTTCTCA CGAGGAGAAG
    GACCTGTTTT TTAACTTGAC GGTCAATCAA GGATTTCTTT CCAATAGCTA CATCATGGAG
    AAGAGATATG GGAACCTCTC CCATGTTAAG ATGATGGCTT CCTCTGCCCC CCTCTGCCAT
    CTCAGTGGCA CGGTTCTACA GCAGGGCACC AGAGTTGGGA CGGCAGCCCT CAGTGCCTGC
    CATGGACTGA CTGGATTTTT CCAACTACCA CATGGAGACT TTTTCATTGA ACCCGTGAAG
    AAGCATCCAC TGGTTGAGGG AGGGTACCAC CCGCACATCG TTTACAGGAG GCAGAAAGTT
    CCAGAAACCA AGGAGCCAAC CTGTGGATTA AAGGACAGTG TTAACATCTC CCAGAAGCAA
    GAGCTATGGC GGGAGAAGTG GGAGAGGCAC AACTTGCCAA GCAGAAGCCT CTCTCGGCGT
    TCCATCAGCA AGGAGAGATG GGTGGAGACA CTGGTGGTGG CCGACACAAA GATGATTGAA
    TACCATGGGA GTGAGAATGT GGAGTCCTAC ATCCTCACCA TCATGAACAT GGTCACTGGG
    TTGTTCCATA ACCCAAGCAT TGGCAATGCA ATTCACATTG TTGTGGTTCG GCTCATTCTA
    CTCGAAGAAG AAGAGCAAGG ACTGAAAATA GTTCACCATG CAGAAAAGAC ACTGTCTAGC
    TTCTGCAAGT GGCAGAAGAG TATCAATCCC AAGAGTGACC TCAATCCTGT TCATCACGAC
    GTGGCTGTCC TTCTCACCAG AAAGGACATC TGTGCTGGTT TCAATCGCCC CTGCGAGACC
    CTGGGCCTGT CTCACCTTTC AGGAATGTGT CAGCCTCACC GCAGTTGTAA CATCAATGAA
    GATTCGGGAC TCCCTCTGGC TTTCACAATT GCCCATGAGC TAGGACACAG CTTCGGCATC
    CAGCATGATG GGAAAGAAAA TGACTGTGAG CCTGTGGGCA GACATCCGTA CATCATGTCC
    CGCCAGCTCC AGTACGATCC CACTCCGCTG ACATGGTCCA AGTGCAGCGA GGAGTACATC
    ACCCGCTTCT TGGACCGAGG CTGGGGGTTC TGTCTTGATG ACATACCTAA AAAGAAAGGC
    TTGAAGTCCA AGGTCATTGC CCCCGGAGTG ATCTATGATG TTCACCACCA GTGCCAGCTA
    CAATATGGAC CCAATGCTAC CTTCTGCCAG GAAGTAGAAA ACGTCTGCCA GACACTGTGG
    TGCTCCGTGA AGGGCTTTTG TCGCTCTAAG CTGGACGCTG CTGCAGATGG AACTCAATGT
    GGTGAGAAGA AGTGGTGTAT GGCAGGCAAG TGCATCACAG TGGGGAAGAA ACCAGAGAGC
    ATTCCTGGAG GCTGGGGCCG CTGGTCACCC TGGTCCCACT GTTCCAGGAC CTGTGGGGCT
    GGAGTCCAGA GCGCAGAGAG GCTCTGCAAC AACCCCGAGC CAAAGTTTGG AGGGAAATAT
    TGCACTGGAG AAAGAAAACG CTATCGCTTG TGCAACGTCC ACCCCTGTCG CTCAGAGGCA
    CCAACATTTC GGCAGATGCA GTGCAGTGAA TTTGACACTG TTCCCTACAA GAATGAACTC
    TACCACTGGT TTCCCATTTT TAACCCAGCA CATCCTTGTG AGCTCTACTG CCGACCCATA
    GATGGCCAGT TTTCTGAGAA AATGCTGGAT GCTGTCATTG ATGGTACCCC TTGCTTTGAA
    GGCGGCAACA GCAGAAATGT CTGTATTAAT GGCATATGTA AGATGGTTGG CTGTGACTAT
    GAGATCGATT CCAATGCCAC CGAGGATCGC TGCGGTGTGT GCCTGGGAGA TGGCTCTTCC
    TGCCAGACTG TGAGAAAGAT GTTTAAGCAG AAGGAAGGAT CTGGTTATGT TGACATTGGG
    CTCATTCCAA AAGGAGCAAG GGACATAAGA GTGATGGAAA TTGAGGGAGC TGGAAACTTC
    CTGGCCATCA GGAGTGAAGA TCCTGAAAAA TATTACCTGA ATGGAGGGTT TATTATCCAG
    TGGAACGGGA ACTATAAGCT GGCAGGGACT GTCTTTCAGT ATGACAGGAA AGGAGACCTG
    GAAAAGCTGA TGGCCACAGG TCCCACCAAT GAGTCTGTGT GGATCCAGCT TCTATTCCAG
    GTGACTAACC CTGGCATCAA GTATGAGTAC ACAATCCAGA AAGATGGCCT TGACAATGAT
    GTTGAGCAGC AGATGTACTT CTGGCAGTAC GGCCACTGGA CAGAGTGCAG TGTGACCTGC
    GGGACAGGTA TCCGCCGCCA AACTGCCCAT TGCATAAAGA AGGGCCGCGG GATGGTGAAA
    GCTACATTCT GTGACCCAGA AACACAGCCC AATGGGAGAC AGAAGAAGTG CCATGAAAAG
    GCTTGTCCAC CCAGGTGGTG GGCAGGGGAG TGGGAAGCAT GCTCGGCGAC ATGCGGGCCC
    CACGGGGAGA AGAAGCGAAC CGTGCTGTGC ATCCAGACCA TGGTCTCTGA CGAGCAGGCT
    CTCCCGCCCA CAGACTGCCA GCACCTGCTG AAGCCCAAGA CCCTCCTTTC CTGCAACAGA
    GACATCCTGT GCCCCTCGGA CTGGACAGTG GGCAACTGGA GTGAGTGTTC TGTTTCCTGT
    GGTGGTGGAG TGCGGATTCG CAGTGTCACA TGTGCCAAGA ACCATGATGA ACCTTGCGAT
    GTGACAAGGA AACCCAACAG CCGAGCTCTG TGTGGCCTCC AGCAATGCCC TTCTAGCCGG
    AGAGTTCTGA AACCAAACAA AGGCACTATT TCCAATGGAA AAAACCCACC AACACTAAAG
    CCCGTCCCTC CACCTACATC CAGGCCCAGA ATGCTGACCA CACCCACAGG GCCTGAGTCT
    ATGAGCACAA GCACTCCAGC AATCAGCAGC CCTAGTCCTA CCACAGCCTC CAAAGAAGGA
    GACCTGGGTG GGAAACAGTG GCAAGATAGC TCAACCCAAC CTGAGCTGAG CTCTCGCTAT
    CTCATTTCCA CTGGAAGCAC TTCCCAGCCC ATCCTCACTT CCCAATCCTT GAGCATTCAG
    CCAAGTGAGG AAAATGTTTC CAGTTCAGAT ACTGGTCCTA CCTCGGAGGG AGGCCTTGTA
    GCTACAACAA CAAGTGGTTC TGGCTTGTCA TCTTCCCGCA ACCCTATCAC TTGGCCTGTG
    ACTCCATTTT ACAATACCTT GACCAAAGGT CCAGAAATGG AGATTCACAG TGGCTCAGGG
    GAAGAAAGAG AACAGCCTGA GGACAAAGAT GAAAGCAATC CTGTAATATG GACCAAGATC
    AGAGTACCTG GAAATGACGC TCCAGTGGAA AGTACAGAAA TGCCACTTGC ACCTCCACTA
    ACACCAGATC TCAGCAGGGA GTCCTGGTGG CCACCCTTCA GCACAGTAAT GGAAGGACTG
    CTCCCCAGCC AAAGGCCCAC TACTTCCGAA ACTGGGACAC CCAGAGTTGA GGGGATGGTT
    ACTGAAAAGC CAGCCAACAC TCTGCTCCCT CTGGGAGGAG ACCACCAGCC AGAACCCTCA
    GGAAAGACGG CAAACCGTAA CCACCTGAAA CTTCCAAACA ACATGAACCA AACAAAAAGT
    TCTGAACCAG TCCTGACTGA GGAGGATGCA ACAAGTCTGA TTACTGAGGG CTTTTTGCTA
    AATGCCTCCA ATTACAAGCA GCTCACAAAC GGCCACGGCT CTGCACACTG GATCGTCGGA
    AACTGGAGCG AGTGCTCCAC CACATGTGGC CTGGGGGCCT ACTGGAGAAG GGTGGAGTGC
    AGCACCCAGA TGGATTCTGA CTGTGCGGCC ATCCAGAGAC CTGACCCTGC AAAAAGATGC
    CACCTCCGTC CCTGTGCTGG CTGGAAAGTG GGAAACTGGA GCAAGTGCTC CAGAAACTGC
    AGTGGGGGCT TCAAGATACG CGAGATTCAG TGCGTGGACA GCCGGGACCA CCGGAACCTG
    AGGCCATTTC ACTGCCAGTT CCTGGCCGGC ATTCCTCCCC CATTGAGCAT GAGCTGTAAC
    CCGGAGCCCT GTGAGGCGTG GCAGGTGGAG CCTTGGAGCC AGTGCTCCAG GTCCTGTGGA
    GGTGGAGTTC AGGAGAGAGG AGTGTTCTGT CCAGGAGGCC TCTGTGATTG GACAAAAAGA
    CCCACATCCA CCATGTCTTG CAATGAGCAC CTGTGCTGTC ACTGGGCCAC TGGGAACTGG
    GACCTGTGTT CCACTTCCTG TGGAGGTGGC TTTCAGAAGA GGACTGTCCA ATGTGTGCCC
    TCAGAGGGCA ATAAAACTGA AGACCAAGAC CAATGTCTAT GTGATCACAA ACCCAGACCT
    CCAGAATTCA AAAAATGCAA CCAGCAGGCC TGCAAGAAAA GTGCCGATTT ACTTTGCACT
    AAGGACAAAC TGTCAGCCAG TTTCTGCCAG ACACTGAAAG CCATGAAGAA ATGTTCTGTG
    CCCACCGTGA GGGCTGAGTG CTGCTTCTCG TGTCCCCAGA CACACATCAC ACACACCCAA
    AGGCAAAGAA GGCAACGGTT GCTCCAAAAG TCAAAAGAAC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.