EFHD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFHD1
Entrez Gene ID	80303
Full Name	EF-hand domain family member D1
Synonyms	MST133,MSTP133,PP3051,SWS2
General protein information	Preferred Names EF-hand domain family member D1 Names EF-hand domain-containing protein D1 EF-hand domain-containing protein 1 swiprosin-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016].
Disorder MIM:	611617

mRNA and Protein(s)

mRNA	Protein	Name
XM_011511924.2	XP_011510226.1	EF-hand domain-containing protein D1 isoform X1
NM_025202.3	NP_079478.1	EF-hand domain-containing protein D1 isoform 1
NM_001308395.2	NP_001295324.1	EF-hand domain-containing protein D1 isoform 3
NM_001308395.1	NP_001295324.1	EF-hand domain-containing protein D1 isoform 3
NM_025202.4	NP_079478.1	EF-hand domain-containing protein D1 isoform 1
NM_001243252.1	NP_001230181.1	EF-hand domain-containing protein D1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Efhd1	NP_083165.1
Rattus norvegicus (Norway rat)	Efhd1	NP_001102780.1
Gallus gallus (chicken)	EFHD1	NP_001026943.1
Danio rerio (zebrafish)	efhd1	XP_683253.1
Canis lupus familiaris (dog)	EFHD1	XP_005635912.1
	EFHD1	XP_001109340.2
Homo sapiens (human)	EFHD1	NP_079478.1
Bos taurus (cattle)	EFHD1	NP_001069300.1
Pan troglodytes (chimpanzee)	EFHD1	XP_001143163.2
Xenopus tropicalis (tropical clawed frog)	efhd1	NP_989300.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of EFHD1 as a novel Ca(2+) sensor for mitoflash activation.
Hou T, Jian C, Xu J, Huang AY, Xi J, Hu K, Wei L, Cheng H, Wang X
Cell calcium59(5)262-70(2016 05)

Aberrant promoter methylation of PPP1R3C and EFHD1 in plasma of colorectal cancer patients.
Takane K, Midorikawa Y, Yagi K, Sakai A, Aburatani H, Takayama T, Kaneda A
Cancer medicine3(5)1235-45(2014 Oct)

Fraternal twins: Swiprosin-1/EFhd2 and Swiprosin-2/EFhd1, two homologous EF-hand containing calcium binding adaptor proteins with distinct functions.
D?tting S, Brachs S, Mielenz D
Cell communication and signaling : CCS92(2011 Jan)

Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53(-/-) mouse.
Tominaga M, Tomooka Y
Biochemical and biophysical research communications297(3)473-9(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EFHD1 functions as a novel mitochondrial Ca(2+) sensor underlying Ca(2+)-dependent activation of mitoflashes
Title: Identification of EFHD1 as a novel Ca(2+) sensor for mitoflash activation.

detection of methylation of PPP1R3C alone or in combination with EFHD1 in plasma DNA showed high sensitivity and specificity in CRC detection, and may be useful detection method for CRC, especially for early-stage CRCs.
Title: Aberrant promoter methylation of PPP1R3C and EFHD1 in plasma of colorectal cancer patients.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Cloning of novel mouse genes associated with neuronal function, including Efhd1.
Title: Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53(-/-) mouse.

The following EFHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01220	NM_025202.4 Latest version!	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu72736	NM_001308395.2 Latest version!	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu72736	XM_011511924.2 Latest version!	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu02227	NM_001243252.1 Latest version!	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu72736	NM_001308395.1	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu01220	NM_025202.3	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFHD1 ( NM_025202.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079478.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01220
Clone ID Related Accession (Same CDS sequence)	NM_025202.3 , NM_025202.4
Accession Version	NM_025202.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC328084.1, AC073254.6, BC002449.2, AC064852.4 and AF218006.1. On May 15, 2009 this sequence version replaced NM_025202.2. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF218006.1, BC035476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGTG AGGAGCTGGC GTGCAAGCTG GAGCGCCGGC TGCGGCGCGA GGAGGCCGAG 
GAGAGTGGCC CCCAGCTGGC TCCCCTCGGC GCCCCAGCCC CGGAGCCCAA GCCCGAGCCC 
GAGCCTCCCG CCCGTGCGCC CACGGCCAGC GCCGACGCGG AGCTGAGCGC CCAGCTGAGC 
CGGCGGCTGG ACATCAACGA GGGCGCTGCG CGGCCCCGGC GCTGCAGGGT CTTCAACCCC 
TACACGGAGT TCCCGGAGTT CAGCCGCCGC CTCATCAAGG ACCTGGAGAG CATGTTCAAA 
CTGTATGACG CTGGGCGGGA TGGCTTCATC GACCTGATGG AGCTGAAGCT GATGATGGAG 
AAGCTGGGGG CCCCCCAGAC CCACCTGGGC CTGAAGAGCA TGATCAAGGA GGTGGATGAG 
GACTTCGATG GCAAGCTCAG CTTCCGGGAG TTCCTGCTCA TTTTCCACAA GGCCGCGGCA 
GGGGAGCTGC AGGAGGACAG TGGGCTGATG GCGCTGGCAA AGCTTTCTGA GATCGATGTG 
GCCCTGGAGG GTGTCAAAGG TGCCAAGAAC TTCTTTGAAG CCAAGGTCCA AGCCTTGTCA 
TCGGCCAGTA AGTTTGAAGC AGAGTTGAAA GCTGAGCAAG ATGAGCGGAA GCGGGAGGAG 
GAGGAGAGGC GGCTCCGCCA GGCAGCCTTC CAGAAACTCA AGGCCAACTT CAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079478.1
CDS	209..928
Translation	MASEELACKLERRLRREEAEESGPQLAPLGAPAPEPKPEPEPPARAPTASADAELSAQLSRRLDINEGAARPRRCRVFNPYTEFPEFSRRLIKDLESMFKLYDAGRDGFIDLMELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	NM_025202.3
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025202.3

ORF Insert Sequence:

ATGGCCAGTG AGGAGCTGGC GTGCAAGCTG GAGCGCCGGC TGCGGCGCGA GGAGGCCGAG 
GAGAGTGGCC CCCAGCTGGC TCCCCTCGGC GCCCCAGCCC CGGAGCCCAA GCCCGAGCCC 
GAGCCTCCCG CCCGTGCGCC CACGGCCAGC GCCGACGCGG AGCTGAGCGC CCAGCTGAGC 
CGGCGGCTGG ACATCAACGA GGGCGCTGCG CGGCCCCGGC GCTGCAGGGT CTTCAACCCC 
TACACGGAGT TCCCGGAGTT CAGCCGCCGC CTCATCAAGG ACCTGGAGAG CATGTTCAAA 
CTGTATGACG CTGGGCGGGA TGGCTTCATC GACCTGATGG AGCTGAAGCT GATGATGGAG 
AAGCTGGGGG CCCCCCAGAC CCACCTGGGC CTGAAGAGCA TGATCAAGGA GGTGGATGAG 
GACTTCGATG GCAAGCTCAG CTTCCGGGAG TTCCTGCTCA TTTTCCACAA GGCCGCGGCA 
GGGGAGCTGC AGGAGGACAG TGGGCTGATG GCGCTGGCAA AGCTTTCTGA GATCGATGTG 
GCCCTGGAGG GTGTCAAAGG TGCCAAGAAC TTCTTTGAAG CCAAGGTCCA AGCCTTGTCA 
TCGGCCAGTA AGTTTGAAGC AGAGTTGAAA GCTGAGCAAG ATGAGCGGAA GCGGGAGGAG 
GAGGAGAGGC GGCTCCGCCA GGCAGCCTTC CAGAAACTCA AGGCCAACTT CAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFHD1 ( NM_025202.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079478.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01220
Clone ID Related Accession (Same CDS sequence)	NM_025202.3 , NM_025202.4
Accession Version	NM_025202.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073254.6, BC002449.2, AC064852.4 and AF218006.1. On Nov 22, 2018 this sequence version replaced NM_025202.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC035476.1, SRR3476690.420233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264059.8/ ENSP00000264059.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAGTG AGGAGCTGGC GTGCAAGCTG GAGCGCCGGC TGCGGCGCGA GGAGGCCGAG 
GAGAGTGGCC CCCAGCTGGC TCCCCTCGGC GCCCCAGCCC CGGAGCCCAA GCCCGAGCCC 
GAGCCTCCCG CCCGTGCGCC CACGGCCAGC GCCGACGCGG AGCTGAGCGC CCAGCTGAGC 
CGGCGGCTGG ACATCAACGA GGGCGCTGCG CGGCCCCGGC GCTGCAGGGT CTTCAACCCC 
TACACGGAGT TCCCGGAGTT CAGCCGCCGC CTCATCAAGG ACCTGGAGAG CATGTTCAAA 
CTGTATGACG CTGGGCGGGA TGGCTTCATC GACCTGATGG AGCTGAAGCT GATGATGGAG 
AAGCTGGGGG CCCCCCAGAC CCACCTGGGC CTGAAGAGCA TGATCAAGGA GGTGGATGAG 
GACTTCGATG GCAAGCTCAG CTTCCGGGAG TTCCTGCTCA TTTTCCACAA GGCCGCGGCA 
GGGGAGCTGC AGGAGGACAG TGGGCTGATG GCGCTGGCAA AGCTTTCTGA GATCGATGTG 
GCCCTGGAGG GTGTCAAAGG TGCCAAGAAC TTCTTTGAAG CCAAGGTCCA AGCCTTGTCA 
TCGGCCAGTA AGTTTGAAGC AGAGTTGAAA GCTGAGCAAG ATGAGCGGAA GCGGGAGGAG 
GAGGAGAGGC GGCTCCGCCA GGCAGCCTTC CAGAAACTCA AGGCCAACTT CAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079478.1
CDS	102..821
Translation	MASEELACKLERRLRREEAEESGPQLAPLGAPAPEPKPEPEPPARAPTASADAELSAQLSRRLDINEGAARPRRCRVFNPYTEFPEFSRRLIKDLESMFKLYDAGRDGFIDLMELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	NM_025202.4
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025202.4

ORF Insert Sequence:

ATGGCCAGTG AGGAGCTGGC GTGCAAGCTG GAGCGCCGGC TGCGGCGCGA GGAGGCCGAG 
GAGAGTGGCC CCCAGCTGGC TCCCCTCGGC GCCCCAGCCC CGGAGCCCAA GCCCGAGCCC 
GAGCCTCCCG CCCGTGCGCC CACGGCCAGC GCCGACGCGG AGCTGAGCGC CCAGCTGAGC 
CGGCGGCTGG ACATCAACGA GGGCGCTGCG CGGCCCCGGC GCTGCAGGGT CTTCAACCCC 
TACACGGAGT TCCCGGAGTT CAGCCGCCGC CTCATCAAGG ACCTGGAGAG CATGTTCAAA 
CTGTATGACG CTGGGCGGGA TGGCTTCATC GACCTGATGG AGCTGAAGCT GATGATGGAG 
AAGCTGGGGG CCCCCCAGAC CCACCTGGGC CTGAAGAGCA TGATCAAGGA GGTGGATGAG 
GACTTCGATG GCAAGCTCAG CTTCCGGGAG TTCCTGCTCA TTTTCCACAA GGCCGCGGCA 
GGGGAGCTGC AGGAGGACAG TGGGCTGATG GCGCTGGCAA AGCTTTCTGA GATCGATGTG 
GCCCTGGAGG GTGTCAAAGG TGCCAAGAAC TTCTTTGAAG CCAAGGTCCA AGCCTTGTCA 
TCGGCCAGTA AGTTTGAAGC AGAGTTGAAA GCTGAGCAAG ATGAGCGGAA GCGGGAGGAG 
GAGGAGAGGC GGCTCCGCCA GGCAGCCTTC CAGAAACTCA AGGCCAACTT CAATACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFHD1 ( NM_001308395.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295324.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu72736
Clone ID Related Accession (Same CDS sequence)	XM_011511924.2 , NM_001308395.2 , NM_001308395.1
Accession Version	NM_001308395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC328084.1, AK315442.1, BI460146.1, CR627385.1, BU731354.1 and DB570289.1. On May 31, 2019 this sequence version replaced NM_001308395.1. Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream start codon compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream start codon to encode an isoform (3) that has a significantly shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BI460146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295324.1
CDS	517..900
Translation	MELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	NM_001308395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308395.2

ORF Insert Sequence:

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFHD1 ( NM_001308395.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295324.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu72736
Clone ID Related Accession (Same CDS sequence)	XM_011511924.2 , NM_001308395.2 , NM_001308395.1
Accession Version	NM_001308395.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC328084.1, AC073254.6, AK315442.1, BI460146.1, CR627385.1, BU731354.1, DB570289.1 and AW271276.1. Transcript Variant: This variant (3) contains an alternate exon in its 5' UTR compared to variant 1. This variant represents translation initiation at a downstream start codon compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream start codon to encode an isoform (3) that has a significantly shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BI460146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295324.1
CDS	624..1007
Translation	MELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	NM_001308395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308395.1

ORF Insert Sequence:

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFHD1 ( XM_011511924.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510226.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu72736
Clone ID Related Accession (Same CDS sequence)	XM_011511924.2 , NM_001308395.2 , NM_001308395.1
Accession Version	XM_011511924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510226.1
CDS	393..776
Translation	MELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	XM_011511924.2
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511924.2

ORF Insert Sequence:

ATGGAGCTGA AGCTGATGAT GGAGAAGCTG GGGGCCCCCC AGACCCACCT GGGCCTGAAG 
AGCATGATCA AGGAGGTGGA TGAGGACTTC GATGGCAAGC TCAGCTTCCG GGAGTTCCTG 
CTCATTTTCC ACAAGGCCGC GGCAGGGGAG CTGCAGGAGG ACAGTGGGCT GATGGCGCTG 
GCAAAGCTTT CTGAGATCGA TGTGGCCCTG GAGGGTGTCA AAGGTGCCAA GAACTTCTTT 
GAAGCCAAGG TCCAAGCCTT GTCATCGGCC AGTAAGTTTG AAGCAGAGTT GAAAGCTGAG 
CAAGATGAGC GGAAGCGGGA GGAGGAGGAG AGGCGGCTCC GCCAGGCAGC CTTCCAGAAA 
CTCAAGGCCA ACTTCAATAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFHD1 ( NM_001243252.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230181.1 Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 2, mRNA.

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CloneID	OHu02227
Clone ID Related Accession (Same CDS sequence)	NM_001243252.1
Accession Version	NM_001243252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	EF-hand domain-containing protein D1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310565.1, DB461847.1, BM677018.1 and AW271276.1. This sequence is a reference standard in the RefSeqGene project. On Aug 6, 2011 this sequence version replaced NR_027663.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and uses an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK310565.1, SRR1803616.241668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04284274 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAGT TGCAGTATGA CGCTGGGCGG GATGGCTTCA TCGACCTGAT GGAGCTGAAG 
CTGATGATGG AGAAGCTGGG GGCCCCCCAG ACCCACCTGG GCCTGAAGAG CATGATCAAG 
GAGGTGGATG AGGACTTCGA TGGCAAGCTC AGCTTCCGGG AGTTCCTGCT CATTTTCCAC 
AAGGCCGCGG CAGGGGAGCT GCAGGAGGAC AGTGGGCTGA TGGCGCTGGC AAAGCTTTCT 
GAGATCGATG TGGCCCTGGA GGGTGTCAAA GGTGCCAAGA ACTTCTTTGA AGCCAAGGTC 
CAAGCCTTGT CATCGGCCAG TAAGTTTGAA GCAGAGTTGA AAGCTGAGCA AGATGAGCGG 
AAGCGGGAGG AGGAGGAGAG GCGGCTCCGC CAGGCAGCCT TCCAGAAACT CAAGGCCAAC 
TTCAATACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230181.1
CDS	104..535
Translation	MGELQYDAGRDGFIDLMELKLMMEKLGAPQTHLGLKSMIKEVDEDFDGKLSFREFLLIFHKAAAGELQEDSGLMALAKLSEIDVALEGVKGAKNFFEAKVQALSSASKFEAELKAEQDERKREEEERRLRQAAFQKLKANFNT

Target ORF information:

RefSeq Version	NM_001243252.1
Organism	Homo sapiens(human)
Definition	Homo sapiens EF-hand domain family member D1 (EFHD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243252.1

ORF Insert Sequence:

ATGGGAGAGT TGCAGTATGA CGCTGGGCGG GATGGCTTCA TCGACCTGAT GGAGCTGAAG 
CTGATGATGG AGAAGCTGGG GGCCCCCCAG ACCCACCTGG GCCTGAAGAG CATGATCAAG 
GAGGTGGATG AGGACTTCGA TGGCAAGCTC AGCTTCCGGG AGTTCCTGCT CATTTTCCAC 
AAGGCCGCGG CAGGGGAGCT GCAGGAGGAC AGTGGGCTGA TGGCGCTGGC AAAGCTTTCT 
GAGATCGATG TGGCCCTGGA GGGTGTCAAA GGTGCCAAGA ACTTCTTTGA AGCCAAGGTC 
CAAGCCTTGT CATCGGCCAG TAAGTTTGAA GCAGAGTTGA AAGCTGAGCA AGATGAGCGG 
AAGCGGGAGG AGGAGGAGAG GCGGCTCCGC CAGGCAGCCT TCCAGAAACT CAAGGCCAAC 
TTCAATACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of EFHD1 as a novel Ca(2+) sensor for mitoflash activation.
Cell calcium59(5)262-70(2016 05)
Hou T,Jian C,Xu J,Huang AY,Xi J,Hu K,Wei L,Cheng H,Wang X

Aberrant promoter methylation of PPP1R3C and EFHD1 in plasma of colorectal cancer patients.
Cancer medicine3(5)1235-45(2014 Oct)
Takane K,Midorikawa Y,Yagi K,Sakai A,Aburatani H,Takayama T,Kaneda A

Fraternal twins: Swiprosin-1/EFhd2 and Swiprosin-2/EFhd1, two homologous EF-hand containing calcium binding adaptor proteins with distinct functions.
Cell communication and signaling : CCS92(2011 Jan)
D?tting S,Brachs S,Mielenz D

Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53(-/-) mouse.
Biochemical and biophysical research communications297(3)473-9(2002 Sep)
Tominaga M,Tomooka Y

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EFHD1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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