PIF1 cDNA ORF clone, Homo sapiens(human)

The following PIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09857 NM_025049.4
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu09857 NM_001286496.2
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu09857 XM_011522083.2
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu09857 XM_011522084.2
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu00975 NM_001286497.2
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu10139 NM_001286499.2
Latest version!
Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OHu09857 NM_001286496.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu00975 NM_001286497.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
OHu09857 NM_025049.3 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu10139 NM_001286499.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version NM_001286496.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1, BC033254.1 and AW170361.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273425.1
    CDS120..2045
    Translation

    Target ORF information:

    RefSeq Version NM_001286496.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version NM_025049.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1 and AC091582.17. On May 31, 2019 this sequence version replaced NM_025049.3. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185926.1, BC137503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079325.2
    CDS75..2000
    Translation

    Target ORF information:

    RefSeq Version NM_025049.4
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025049.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version NM_001286496.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1 and BC033254.1. On Nov 22, 2018 this sequence version replaced NM_001286496.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 MANE Ensembl match :: ENST00000559239.2/ ENSP00000452792.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273425.1
    CDS99..2024
    Translation

    Target ORF information:

    RefSeq Version NM_001286496.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version NM_025049.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, AC091582.17 and AW170361.1. On Nov 7, 2013 this sequence version replaced NM_025049.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185926.1, BC137503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079325.2
    CDS96..2021
    Translation

    Target ORF information:

    RefSeq Version NM_025049.3
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025049.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version XM_011522083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520385.1
    CDS212..2137
    Translation

    Target ORF information:

    RefSeq Version XM_011522083.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09857
    Clone ID Related Accession (Same CDS sequence) NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
    Accession Version XM_011522084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1926bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520386.1
    CDS44..1969
    Translation

    Target ORF information:

    RefSeq Version XM_011522084.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011522084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00975
    Clone ID Related Accession (Same CDS sequence) NM_001286497.2 , NM_001286497.1
    Accession Version NM_001286497.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185927.1 and EU084033.1. On May 31, 2019 this sequence version replaced NM_001286497.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and contains an alternate exon structure in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as beta) has a distinct C-terminus and is longer than isoform a. This isoform localizes to the mitochondria (PMID: 17827721). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT
    CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT
    AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC
    CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG
    ATAAATAGTG TTTGGGGACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273426.1
    CDS75..2198
    Translation

    Target ORF information:

    RefSeq Version NM_001286497.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286497.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT
    CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT
    AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC
    CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG
    ATAAATAGTG TTTGGGGACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00975
    Clone ID Related Accession (Same CDS sequence) NM_001286497.2 , NM_001286497.1
    Accession Version NM_001286497.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185927.1, EU084033.1 and AW170361.1. On Nov 7, 2013 this sequence version replaced XM_005254691.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and contains an alternate exon structure in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as beta) has a distinct C-terminus and is longer than isoform a. This isoform localizes to the mitochondria (PMID: 17827721). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT
    CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT
    AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC
    CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG
    ATAAATAGTG TTTGGGGACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273426.1
    CDS96..2219
    Translation

    Target ORF information:

    RefSeq Version NM_001286497.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286497.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
    GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC
    GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA
    GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC
    GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA
    GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG
    CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG
    GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC
    AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT
    GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG
    CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA
    GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA
    ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC
    TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT
    CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC
    CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG
    GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC
    TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC
    TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG
    AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA
    GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT
    GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT
    CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC
    AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG
    GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG
    GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC
    ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT
    CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG
    ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG
    GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG
    GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC
    AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT
    CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT
    AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC
    CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG
    ATAAATAGTG TTTGGGGACA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10139
    Clone ID Related Accession (Same CDS sequence) NM_001286499.1 , NM_001286499.2
    Accession Version NM_001286499.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1, BC033254.1 and AW170361.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (c, also known as alpha-54) initiates translation from a downstream AUG. This isoform localizes to the mitochondria (PMID: 23275553). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:23275553) gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
    GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC
    GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG
    CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC
    TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG
    CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG
    CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG
    CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT
    GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG
    AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT
    GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC
    ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG
    CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA
    GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC
    GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC
    TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC
    CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC
    GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC
    AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC
    CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG
    GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT
    CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG
    GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG
    GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC
    ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC
    ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT
    GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG
    CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC
    CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC
    CAGGAGAACA TGGACCCAAT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273428.1
    CDS279..2045
    Translation

    Target ORF information:

    RefSeq Version NM_001286499.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
    GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC
    GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG
    CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC
    TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG
    CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG
    CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG
    CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT
    GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG
    AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT
    GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC
    ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG
    CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA
    GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC
    GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC
    TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC
    CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC
    GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC
    AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC
    CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG
    GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT
    CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG
    GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG
    GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC
    ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC
    ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT
    GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG
    CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC
    CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC
    CAGGAGAACA TGGACCCAAT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10139
    Clone ID Related Accession (Same CDS sequence) NM_001286499.1 , NM_001286499.2
    Accession Version NM_001286499.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product ATP-dependent DNA helicase PIF1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1 and BC033254.1. On May 31, 2019 this sequence version replaced NM_001286499.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (c, also known as alpha-54) initiates translation from a downstream AUG. This isoform localizes to the mitochondria (PMID: 23275553). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:23275553) gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
    GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC
    GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG
    CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC
    TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG
    CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG
    CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG
    CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT
    GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG
    AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT
    GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC
    ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG
    CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA
    GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC
    GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC
    TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC
    CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC
    GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC
    AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC
    CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG
    GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT
    CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG
    GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG
    GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC
    ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC
    ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT
    GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG
    CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC
    CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC
    CAGGAGAACA TGGACCCAAT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273428.1
    CDS258..2024
    Translation

    Target ORF information:

    RefSeq Version NM_001286499.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286499.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
    GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC
    GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG
    CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC
    TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG
    CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG
    CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG
    CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT
    GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG
    AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT
    GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC
    ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG
    CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA
    GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC
    GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC
    TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC
    CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC
    GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC
    AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC
    CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG
    GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT
    CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG
    GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG
    GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC
    ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC
    ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT
    GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG
    CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC
    CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC
    CAGGAGAACA TGGACCCAAT CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.