PIF1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001286496.1	NP_001273425.1	ATP-dependent DNA helicase PIF1 isoform a
NM_025049.4	NP_079325.2	ATP-dependent DNA helicase PIF1 isoform a
NM_001286499.1	NP_001273428.1	ATP-dependent DNA helicase PIF1 isoform c
XM_011522083.2	XP_011520385.1	ATP-dependent DNA helicase PIF1 isoform X1
NM_001286496.2	NP_001273425.1	ATP-dependent DNA helicase PIF1 isoform a
NM_001286497.2	NP_001273426.1	ATP-dependent DNA helicase PIF1 isoform b
XM_011522084.2	XP_011520386.1	ATP-dependent DNA helicase PIF1 isoform X1
NM_001286497.1	NP_001273426.1	ATP-dependent DNA helicase PIF1 isoform b
NM_025049.3	NP_079325.2	ATP-dependent DNA helicase PIF1 isoform a
NM_001286499.2	NP_001273428.1	ATP-dependent DNA helicase PIF1 isoform c

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09857	NM_025049.4 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu09857	NM_001286496.2 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu09857	XM_011522083.2 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu09857	XM_011522084.2 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu00975	NM_001286497.2 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu10139	NM_001286499.2 Latest version!	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu09857	NM_001286496.1	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu00975	NM_001286497.1	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu09857	NM_025049.3	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu10139	NM_001286499.1	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

PIF1 ( NM_001286496.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273425.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	NM_001286496.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1, BC033254.1 and AW170361.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273425.1
CDS	120..2045
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_001286496.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286496.1

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_025049.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079325.2 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	NM_025049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1 and AC091582.17. On May 31, 2019 this sequence version replaced NM_025049.3. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185926.1, BC137503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079325.2
CDS	75..2000
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_025049.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025049.4

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001286496.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273425.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	NM_001286496.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1 and BC033254.1. On Nov 22, 2018 this sequence version replaced NM_001286496.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 MANE Ensembl match :: ENST00000559239.2/ ENSP00000452792.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273425.1
CDS	99..2024
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_001286496.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286496.2

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_025049.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079325.2 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

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CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	NM_025049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, AC091582.17 and AW170361.1. On Nov 7, 2013 this sequence version replaced NM_025049.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185926.1, BC137503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079325.2
CDS	96..2021
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_025049.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025049.3

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_011522083.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520385.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

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CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	XM_011522083.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520385.1
CDS	212..2137
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	XM_011522083.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522083.2

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( XM_011522084.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520386.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

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CloneID	OHu09857
Clone ID Related Accession (Same CDS sequence)	NM_001286496.1 , NM_025049.4 , XM_011522083.2 , NM_001286496.2 , XM_011522084.2 , NM_025049.3
Accession Version	XM_011522084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520386.1
CDS	44..1969
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	XM_011522084.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522084.2

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGAGT CCCCAGATGA TGATGAGGCA GCCTCAGACC AGGAGAACAT GGACCCAATC 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001286497.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273426.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

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CloneID	OHu00975
Clone ID Related Accession (Same CDS sequence)	NM_001286497.2 , NM_001286497.1
Accession Version	NM_001286497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185927.1 and EU084033.1. On May 31, 2019 this sequence version replaced NM_001286497.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and contains an alternate exon structure in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as beta) has a distinct C-terminus and is longer than isoform a. This isoform localizes to the mitochondria (PMID: 17827721). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT 
CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT 
AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC 
CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG 
ATAAATAGTG TTTGGGGACA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273426.1
CDS	75..2198
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLAAEGRGNEDRCSGSSIRALGGDWWGLRLGAASKQRTELRCVSTARPSLAQPRTNTLQSLTKEHKLQNVHPYFKLLFQGINSVWGH

Target ORF information:

RefSeq Version	NM_001286497.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286497.2

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT 
CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT 
AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC 
CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG 
ATAAATAGTG TTTGGGGACA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001286497.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273426.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00975
Clone ID Related Accession (Same CDS sequence)	NM_001286497.2 , NM_001286497.1
Accession Version	NM_001286497.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185927.1, EU084033.1 and AW170361.1. On Nov 7, 2013 this sequence version replaced XM_005254691.1. Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR and contains an alternate exon structure in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as beta) has a distinct C-terminus and is longer than isoform a. This isoform localizes to the mitochondria (PMID: 17827721). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB185927.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT 
CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT 
AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC 
CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG 
ATAAATAGTG TTTGGGGACA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273426.1
CDS	96..2219
Translation	MLSGIEAAAGEYEDSELRCRVAVEELSPGGQPRRRQALRTAELSLGRNERRELMLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLAAEGRGNEDRCSGSSIRALGGDWWGLRLGAASKQRTELRCVSTARPSLAQPRTNTLQSLTKEHKLQNVHPYFKLLFQGINSVWGH

Target ORF information:

RefSeq Version	NM_001286497.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286497.1

ORF Insert Sequence:

ATGCTCTCGG GCATAGAGGC GGCGGCAGGG GAATATGAGG ACTCGGAGCT GCGGTGCCGC 
GTGGCTGTGG AGGAGCTGAG CCCGGGCGGG CAGCCGCGAA GGCGCCAGGC CCTGCGCACC 
GCGGAGCTGA GCCTGGGTCG CAACGAGCGC CGCGAGTTGA TGCTGCGGCT GCAAGCGCCA 
GGGCCCGCGG GGCGGCCGCG CTGCTTTCCT CTGCGCGCCG CGCGCCTCTT CACGCGTTTC 
GCCGAGGCCG GGCGCAGCAC CCTGCGGCTC CCCGCCCACG ACACCCCCGG GGCCGGCGCA 
GTGCAGCTGC TGCTCTCGGA CTGCCCCCCA GACCGCCTGC GCCGCTTCCT GCGCACATTG 
CGCCTCAAGC TGGCTGCGGC CCCGGGTCCC GGGCCGGCCT CCGCCCGAGC GCAGCTGCTG 
GGCCCGCGGC CCCGCGACTT CGTCACCATC AGCCCTGTGC AGCCCGAGGA GCGGCGGCTC 
AGGGCGGCCA CCCGGGTTCC GGACACTACG CTGGTGAAGC GGCCTGTGGA GCCCCAGGCT 
GGGGCCGAGC CTAGCACAGA AGCCCCAAGG TGGCCCCTGC CTGTGAAGAG GCTGAGCTTG 
CCCTCCACCA AGCCACAGCT TTCTGAGGAA CAGGCTGCTG TGCTGAGGGC CGTCCTGAAA 
GGCCAGAGCA TCTTCTTCAC TGGGAGTGCA GGAACAGGGA AGTCATATCT GCTAAAGCGA 
ATCCTGGGCT CACTGCCCCC CACAGGCACT GTGGCCACTG CCAGCACTGG GGTGGCAGCC 
TGCCACATCG GGGGCACCAC CCTCCATGCC TTTGCAGGCA TCGGCTCAGG CCAGGCTCCT 
CTAGCCCAGT GTGTGGCCCT GGCCCAAAGG CCAGGCGTGC GGCAGGGCTG GCTGAACTGC 
CAGCGGTTGG TCATTGACGA GATCTCAATG GTGGAGGCAG ACCTGTTTGA CAAACTGGAG 
GCCGTGGCCA GAGCTGTCCG GCAGCAGAAC AAGCCATTCG GAGGGATCCA GCTCATCATC 
TGTGGGGACT TTCTGCAGCT GCCACCTGTG ACCAAGGGCT CCCAGCCCCC ACGGTTCTGC 
TTCCAGTCCA AGAGCTGGAA GAGGTGTGTG CCAGTGACCC TGGAGCTGAC CAAGGTGTGG 
AGGCAGGCAG ACCAGACCTT CATCTCTCTA CTGCAGGCCG TGAGGCTAGG CAGGTGTTCA 
GATGAGGTGA CCCGCCAGCT CCAGGCCACA GCTTCCCACA AGGTGGGGCG AGATGGGATT 
GTGGCCACGA GGCTCTGCAC CCACCAGGAT GATGTGGCCC TCACCAACGA GAGGCGGCTT 
CAGGAGCTGC CAGGTAAGGT ACACAGATTT GAGGCTATGG ACAGCAACCC TGAGCTGGCC 
AGTACCCTGG ATGCCCAGTG TCCTGTTAGC CAGCTCCTTC AACTAAAGCT GGGGGCCCAG 
GTGATGCTGG TGAAAAACTT ATCGGTGTCT CGGGGCCTGG TGAATGGTGC CCGAGGGGTG 
GTAGTTGGGT TCGAGGCAGA AGGGAGAGGG CTACCCCAGG TGCGGTTCCT GTGTGGAGTC 
ACTGAGGTCA TCCACGCTGA CCGCTGGACG GTGCAGGCCA CCGGGGGCCA GCTCCTCAGT 
CGGCAGCAGC TGCCCCTCCA GCTGGCCTGG GCGATGTCCA TCCACAAGAG CCAAGGCATG 
ACCCTGGATT GTGTGGAGAT TTCTCTGGGC CGTGTGTTTG CCAGTGGCCA GGCCTATGTG 
GCCCTTTCTC GGGCCCGCAG CCTGCAGGGC CTACGTGTGC TGGACTTTGA CCCCATGGCG 
GTTCGCTGTG ACCCCCGTGT GCTGCACTTC TATGCCACCC TGCGGCGGGG CAGGAGCCTC 
AGTCTGGCTG CAGAAGGGAG AGGCAATGAA GACAGGTGCT CCGGAAGCAG CATCAGGGCT 
CTTGGAGGGG ACTGGTGGGG ACTCAGGCTG GGTGCAGCCT CCAAACAGAG AACGGAACTT 
AGGTGTGTCT CTACAGCTAG GCCCAGCCTA GCCCAGCCCA GAACAAACAC CCTTCAGAGC 
CTAACCAAAG AACATAAGCT GCAAAATGTG CACCCATATT TTAAGCTGCT TTTTCAGGGG 
ATAAATAGTG TTTGGGGACA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001286499.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273428.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

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CloneID	OHu10139
Clone ID Related Accession (Same CDS sequence)	NM_001286499.1 , NM_001286499.2
Accession Version	NM_001286499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1, BC033254.1 and AW170361.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (c, also known as alpha-54) initiates translation from a downstream AUG. This isoform localizes to the mitochondria (PMID: 23275553). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:23275553) gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC 
GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG 
CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC 
TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG 
CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG 
CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG 
CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT 
GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG 
AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT 
GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC 
ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG 
CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA 
GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC 
GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC 
TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC 
CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC 
GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC 
AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC 
CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG 
GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT 
CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG 
GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG 
GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC 
ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC 
ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT 
GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG 
CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC 
CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC 
CAGGAGAACA TGGACCCAAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273428.1
CDS	279..2045
Translation	MLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_001286499.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286499.1

ORF Insert Sequence:

ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC 
GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG 
CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC 
TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG 
CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG 
CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG 
CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT 
GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG 
AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT 
GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC 
ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG 
CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA 
GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC 
GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC 
TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC 
CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC 
GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC 
AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC 
CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG 
GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT 
CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG 
GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG 
GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC 
ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC 
ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT 
GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG 
CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC 
CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC 
CAGGAGAACA TGGACCCAAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIF1 ( NM_001286499.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273428.1 Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

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CloneID	OHu10139
Clone ID Related Accession (Same CDS sequence)	NM_001286499.1 , NM_001286499.2
Accession Version	NM_001286499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ATP-dependent DNA helicase PIF1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB185926.1, EU084033.1 and BC033254.1. On May 31, 2019 this sequence version replaced NM_001286499.1. Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (c, also known as alpha-54) initiates translation from a downstream AUG. This isoform localizes to the mitochondria (PMID: 23275553). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU084033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:23275553) gene product(s) localized to mito. :: PMID: 17827721, 23275553 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC 
GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG 
CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC 
TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG 
CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG 
CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG 
CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT 
GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG 
AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT 
GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC 
ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG 
CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA 
GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC 
GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC 
TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC 
CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC 
GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC 
AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC 
CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG 
GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT 
CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG 
GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG 
GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC 
ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC 
ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT 
GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG 
CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC 
CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC 
CAGGAGAACA TGGACCCAAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273428.1
CDS	258..2024
Translation	MLRLQAPGPAGRPRCFPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFVTISPVQPEERRLRAATRVPDTTLVKRPVEPQAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQLPPVTKGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFEAEGRGLPQVRFLCGVTEVIHADRWTVQATGGQLLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLESPDDDEAASDQENMDPIL

Target ORF information:

RefSeq Version	NM_001286499.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PIF1 5'-to-3' DNA helicase (PIF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286499.2

ORF Insert Sequence:

ATGCTGCGGC TGCAAGCGCC AGGGCCCGCG GGGCGGCCGC GCTGCTTTCC TCTGCGCGCC 
GCGCGCCTCT TCACGCGTTT CGCCGAGGCC GGGCGCAGCA CCCTGCGGCT CCCCGCCCAC 
GACACCCCCG GGGCCGGCGC AGTGCAGCTG CTGCTCTCGG ACTGCCCCCC AGACCGCCTG 
CGCCGCTTCC TGCGCACATT GCGCCTCAAG CTGGCTGCGG CCCCGGGTCC CGGGCCGGCC 
TCCGCCCGAG CGCAGCTGCT GGGCCCGCGG CCCCGCGACT TCGTCACCAT CAGCCCTGTG 
CAGCCCGAGG AGCGGCGGCT CAGGGCGGCC ACCCGGGTTC CGGACACTAC GCTGGTGAAG 
CGGCCTGTGG AGCCCCAGGC TGGGGCCGAG CCTAGCACAG AAGCCCCAAG GTGGCCCCTG 
CCTGTGAAGA GGCTGAGCTT GCCCTCCACC AAGCCACAGC TTTCTGAGGA ACAGGCTGCT 
GTGCTGAGGG CCGTCCTGAA AGGCCAGAGC ATCTTCTTCA CTGGGAGTGC AGGAACAGGG 
AAGTCATATC TGCTAAAGCG AATCCTGGGC TCACTGCCCC CCACAGGCAC TGTGGCCACT 
GCCAGCACTG GGGTGGCAGC CTGCCACATC GGGGGCACCA CCCTCCATGC CTTTGCAGGC 
ATCGGCTCAG GCCAGGCTCC TCTAGCCCAG TGTGTGGCCC TGGCCCAAAG GCCAGGCGTG 
CGGCAGGGCT GGCTGAACTG CCAGCGGTTG GTCATTGACG AGATCTCAAT GGTGGAGGCA 
GACCTGTTTG ACAAACTGGA GGCCGTGGCC AGAGCTGTCC GGCAGCAGAA CAAGCCATTC 
GGAGGGATCC AGCTCATCAT CTGTGGGGAC TTTCTGCAGC TGCCACCTGT GACCAAGGGC 
TCCCAGCCCC CACGGTTCTG CTTCCAGTCC AAGAGCTGGA AGAGGTGTGT GCCAGTGACC 
CTGGAGCTGA CCAAGGTGTG GAGGCAGGCA GACCAGACCT TCATCTCTCT ACTGCAGGCC 
GTGAGGCTAG GCAGGTGTTC AGATGAGGTG ACCCGCCAGC TCCAGGCCAC AGCTTCCCAC 
AAGGTGGGGC GAGATGGGAT TGTGGCCACG AGGCTCTGCA CCCACCAGGA TGATGTGGCC 
CTCACCAACG AGAGGCGGCT TCAGGAGCTG CCAGGTAAGG TACACAGATT TGAGGCTATG 
GACAGCAACC CTGAGCTGGC CAGTACCCTG GATGCCCAGT GTCCTGTTAG CCAGCTCCTT 
CAACTAAAGC TGGGGGCCCA GGTGATGCTG GTGAAAAACT TATCGGTGTC TCGGGGCCTG 
GTGAATGGTG CCCGAGGGGT GGTAGTTGGG TTCGAGGCAG AAGGGAGAGG GCTACCCCAG 
GTGCGGTTCC TGTGTGGAGT CACTGAGGTC ATCCACGCTG ACCGCTGGAC GGTGCAGGCC 
ACCGGGGGCC AGCTCCTCAG TCGGCAGCAG CTGCCCCTCC AGCTGGCCTG GGCGATGTCC 
ATCCACAAGA GCCAAGGCAT GACCCTGGAT TGTGTGGAGA TTTCTCTGGG CCGTGTGTTT 
GCCAGTGGCC AGGCCTATGT GGCCCTTTCT CGGGCCCGCA GCCTGCAGGG CCTACGTGTG 
CTGGACTTTG ACCCCATGGC GGTTCGCTGT GACCCCCGTG TGCTGCACTT CTATGCCACC 
CTGCGGCGGG GCAGGAGCCT CAGTCTGGAG TCCCCAGATG ATGATGAGGC AGCCTCAGAC 
CAGGAGAACA TGGACCCAAT CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PIF1
Entrez Gene ID	80119
Full Name	PIF1 5'-to-3' DNA helicase
Synonyms	C15orf20,PIF
General protein information	Preferred Names PIF1 5'-to-3' DNA helicase Names ATP-dependent DNA helicase PIF1 DNA repair and recombination helicase PIF1 PIF1 5'-to-3' DNA helicase homolog PIF1/RRM3 DNA helicase-like protein petite integration frequency 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q22.31
Summary	This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013].
Disorder MIM:	610953

Mus musculus (house mouse)	Pif1	NP_766041.1
Caenorhabditis elegans (roundworm)	pif-1	NP_490774.2
Xenopus tropicalis (tropical clawed frog)	pif1	NP_001016603.1
Homo sapiens (human)	PIF1	NP_001273426.1
	PIF1	XP_001108233.1
Gallus gallus (chicken)	PIF1	XP_426648.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007775	XP_317740.4
Xenopus tropicalis (tropical clawed frog)	LOC101730335	XP_004914011.1
Bos taurus (cattle)	PIF1	NP_001192981.1
Rattus norvegicus (Norway rat)	Pif1	NP_001037718.1
Danio rerio (zebrafish)	pif1	NP_942102.1
Pan troglodytes (chimpanzee)	PIF1	XP_510475.2
Canis lupus familiaris (dog)	PIF1	XP_003640106.1
Drosophila melanogaster (fruit fly)	CG3238	NP_608782.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PIF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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