SPEF2 cDNA ORF clone, Homo sapiens(human)

The following SPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02181 NM_024867.4
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu02105 NM_144722.4
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu39379 XM_005248378.4
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu96094 XM_017009882.2
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu105700 XM_024446219.1
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu39375 XM_005248376.4
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39376 XM_005248377.4
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu72409 XM_011514141.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu72409 XM_017009881.2
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu72410 XM_011514142.1
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu96093 XM_017009880.2
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu72403 XM_011514135.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72404 XM_011514136.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72405 XM_011514137.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72406 XM_011514138.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72407 XM_011514139.3
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72408 XM_011514140.2
Latest version!
Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu39375 XM_005248376.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu72403 XM_011514135.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72407 XM_011514139.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu02105 NM_144722.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu72404 XM_011514136.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72405 XM_011514137.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu96093 XM_017009880.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OHu02181 NM_024867.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu72409 XM_011514141.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu39376 XM_005248377.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu72406 XM_011514138.2 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu72409 XM_017009881.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu96094 XM_017009882.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu39379 XM_005248378.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02181
    Clone ID Related Accession (Same CDS sequence) NM_024867.3 , NM_024867.4
    Accession Version NM_024867.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC067761.1, BC016773.1, BC110984.1, AL832607.1, AK097547.1, AB051557.1, DA295300.1 and BX648691.1. On Apr 25, 2007 this sequence version replaced NM_024867.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA
    AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA
    AAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079143.3
    CDS112..5580
    Translation

    Target ORF information:

    RefSeq Version NM_024867.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA
    AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA
    AAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02181
    Clone ID Related Accession (Same CDS sequence) NM_024867.3 , NM_024867.4
    Accession Version NM_024867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-01
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC025448.6, BC067761.1, BC016773.1, BC110984.1, AL832607.1, AK097547.1, AB051557.1, DA295300.1 and BX648691.1. On Nov 22, 2018 this sequence version replaced NM_024867.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: HM005670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356031.8/ ENSP00000348314.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA
    AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA
    AAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079143.3
    CDS136..5604
    Translation

    Target ORF information:

    RefSeq Version NM_024867.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA
    AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA
    AAGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02105
    Clone ID Related Accession (Same CDS sequence) NM_144722.4 , NM_144722.3
    Accession Version NM_144722.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC025448.6, BC016773.1 and AK058124.1. On May 31, 2019 this sequence version replaced NM_144722.3. Transcript Variant: This variant (2) lacks more than half of the 3'-most exons and has a 3' end that extends into an intron compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.68671.1, BC067761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_653323.1
    CDS136..1680
    Translation

    Target ORF information:

    RefSeq Version NM_144722.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144722.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02105
    Clone ID Related Accession (Same CDS sequence) NM_144722.4 , NM_144722.3
    Accession Version NM_144722.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-22
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016773.1, AK058124.1 and AC025448.6. On Apr 25, 2006 this sequence version replaced NM_144722.2. Transcript Variant: This variant (2) lacks more than half of the 3'-most exons and has a 3' end that extends into an intron compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.68671.1, BC067761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_653323.1
    CDS112..1656
    Translation

    Target ORF information:

    RefSeq Version NM_144722.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_144722.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39379
    Clone ID Related Accession (Same CDS sequence) XM_005248378.3 , XM_005248378.4
    Accession Version XM_005248378.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA
    GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248435.1
    CDS206..3124
    Translation

    Target ORF information:

    RefSeq Version XM_005248378.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248378.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA
    GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39379
    Clone ID Related Accession (Same CDS sequence) XM_005248378.3 , XM_005248378.4
    Accession Version XM_005248378.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248378.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA
    GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248435.1
    CDS104..3022
    Translation

    Target ORF information:

    RefSeq Version XM_005248378.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248378.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA
    GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96094
    Clone ID Related Accession (Same CDS sequence) XM_017009882.1 , XM_017009882.2
    Accession Version XM_017009882.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT
    TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT
    GAAGGGCAAG ATGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865371.1
    CDS210..3767
    Translation

    Target ORF information:

    RefSeq Version XM_017009882.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT
    TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT
    GAAGGGCAAG ATGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96094
    Clone ID Related Accession (Same CDS sequence) XM_017009882.1 , XM_017009882.2
    Accession Version XM_017009882.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT
    TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT
    GAAGGGCAAG ATGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865371.1
    CDS104..3661
    Translation

    Target ORF information:

    RefSeq Version XM_017009882.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT
    TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT
    GAAGGGCAAG ATGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105700
    Clone ID Related Accession (Same CDS sequence) XM_024446219.1
    Accession Version XM_024446219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG
    CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA
    AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT
    GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG
    GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA
    AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA
    TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT
    ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT
    GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT
    TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT
    CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT
    CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT
    CCTACTGCAA AAGGAAAACC TCAATCAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGCATAATAG GAAATAAAGC ATTTACTGAC ATTCTGATCG ATTTGGTGAC CCTGAACCTT
    GGCACAAACA ACTTTCCTAG TAATTGGATG CACCTTACCC AACCTGAATT ACAGGAATTA
    ACATCTTTAT TAACAGTCAA CTCCGAGTTC GTGGACTGGC GGAAGTTCCT GTTAGTAACC
    TCAATGCCTT GGCCCATTCC CTTGGAGGAG GAGCTCCTTG AGACCCTTCA GAAGTTCAAG
    GCTGTGGATA AGGAGCAGTT GGGCACCATC ACTTTTGAGC AGTATATGCA GGCTGGTCTG
    TGGTTTACAG GAGATGAAGA TATAAAAATT CCAGAAAATC CTCTTGAACC CCTTCCATTT
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGCTAT TTGCTGACTA TGAGAAGGAT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTACTTTG CTTGCCACCC AGACACCGTG
    GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTTGGAACTC ATGTCTTCCA ACAAGTCAAA
    GCTTCCATTC CAAGTGCAGA AAAGACCTCC TCAACTGATG CAGGTCCAGC TGAGGAATTT
    CCTGAACCTG AGGAAAATGC TGCAAGAGAA GAAAGGAAAT TAAAAGACGA CACGGAGAAA
    AGGGAACAGA AGGATGAAGA AATCCCTGAA AATGCAAACA ATGAAAAGAT GTCCATGGAA
    ACACTACTCA AAGTGTTCAA AGGGGGAAGT GAAGCACAGG ACTCCAATAG ATTTGCCAGC
    CACCTAAAGA TAGAGAACAT TTATGCAGAG GGCTTCATAA AAACATTTCA AGACCTAGGT
    GCCAAGAACC TGGAGCCAAT TGAAGTCGCT GTTCTCTTGA AGCATCCTTT TATTCAAGAC
    CTGATTTCAA ATTATTCAGA CTATAAGTTT CCTGATATTA AAATAATTCT CCAAAGGAGT
    GAACATGTAC AAGGAAGTGA TGGAGAGAGA TCACCTTCAA GACATACAGA GGAAAAGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301987.1
    CDS632..5854
    Translation

    Target ORF information:

    RefSeq Version XM_024446219.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG
    CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA
    AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT
    GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG
    GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA
    AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA
    TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT
    ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT
    GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT
    TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT
    CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT
    CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT
    CCTACTGCAA AAGGAAAACC TCAATCAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGCATAATAG GAAATAAAGC ATTTACTGAC ATTCTGATCG ATTTGGTGAC CCTGAACCTT
    GGCACAAACA ACTTTCCTAG TAATTGGATG CACCTTACCC AACCTGAATT ACAGGAATTA
    ACATCTTTAT TAACAGTCAA CTCCGAGTTC GTGGACTGGC GGAAGTTCCT GTTAGTAACC
    TCAATGCCTT GGCCCATTCC CTTGGAGGAG GAGCTCCTTG AGACCCTTCA GAAGTTCAAG
    GCTGTGGATA AGGAGCAGTT GGGCACCATC ACTTTTGAGC AGTATATGCA GGCTGGTCTG
    TGGTTTACAG GAGATGAAGA TATAAAAATT CCAGAAAATC CTCTTGAACC CCTTCCATTT
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGCTAT TTGCTGACTA TGAGAAGGAT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTACTTTG CTTGCCACCC AGACACCGTG
    GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTTGGAACTC ATGTCTTCCA ACAAGTCAAA
    GCTTCCATTC CAAGTGCAGA AAAGACCTCC TCAACTGATG CAGGTCCAGC TGAGGAATTT
    CCTGAACCTG AGGAAAATGC TGCAAGAGAA GAAAGGAAAT TAAAAGACGA CACGGAGAAA
    AGGGAACAGA AGGATGAAGA AATCCCTGAA AATGCAAACA ATGAAAAGAT GTCCATGGAA
    ACACTACTCA AAGTGTTCAA AGGGGGAAGT GAAGCACAGG ACTCCAATAG ATTTGCCAGC
    CACCTAAAGA TAGAGAACAT TTATGCAGAG GGCTTCATAA AAACATTTCA AGACCTAGGT
    GCCAAGAACC TGGAGCCAAT TGAAGTCGCT GTTCTCTTGA AGCATCCTTT TATTCAAGAC
    CTGATTTCAA ATTATTCAGA CTATAAGTTT CCTGATATTA AAATAATTCT CCAAAGGAGT
    GAACATGTAC AAGGAAGTGA TGGAGAGAGA TCACCTTCAA GACATACAGA GGAAAAGAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39375
    Clone ID Related Accession (Same CDS sequence) XM_005248376.3 , XM_005248376.4
    Accession Version XM_005248376.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005248376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG
    GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA
    TGGAGAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248433.1
    CDS217..5688
    Translation

    Target ORF information:

    RefSeq Version XM_005248376.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248376.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG
    GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA
    TGGAGAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39375
    Clone ID Related Accession (Same CDS sequence) XM_005248376.3 , XM_005248376.4
    Accession Version XM_005248376.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248376.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG
    GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA
    TGGAGAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248433.1
    CDS104..5575
    Translation

    Target ORF information:

    RefSeq Version XM_005248376.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248376.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG
    AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG
    GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA
    GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG
    TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT
    GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT
    CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG
    AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC
    ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA
    GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG
    GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG
    GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC
    ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT
    GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC
    CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT
    CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG
    GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA
    TGGAGAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39376
    Clone ID Related Accession (Same CDS sequence) XM_005248377.4 , XM_005248377.3
    Accession Version XM_005248377.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248377.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA
    GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC
    AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA
    TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT
    TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT
    AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG
    CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC
    TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT
    CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT
    GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG
    AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC
    AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG
    ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC
    CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT
    GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248434.1
    CDS104..5560
    Translation

    Target ORF information:

    RefSeq Version XM_005248377.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248377.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA
    GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC
    AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA
    TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT
    TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT
    AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG
    CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC
    TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT
    CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT
    GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG
    AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC
    AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG
    ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC
    CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT
    GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39376
    Clone ID Related Accession (Same CDS sequence) XM_005248377.4 , XM_005248377.3
    Accession Version XM_005248377.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA
    GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC
    AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA
    TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT
    TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT
    AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG
    CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC
    TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT
    CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT
    GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG
    AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC
    AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG
    ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC
    CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT
    GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005248434.1
    CDS217..5673
    Translation

    Target ORF information:

    RefSeq Version XM_005248377.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005248377.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA
    GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC
    AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA
    TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT
    TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT
    AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA
    GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG
    CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC
    TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT
    CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT
    GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG
    AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC
    AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG
    ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC
    CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT
    GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72409
    Clone ID Related Accession (Same CDS sequence) XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
    Accession Version XM_011514141.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512443.1
    CDS1253..7120
    Translation

    Target ORF information:

    RefSeq Version XM_011514141.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514141.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72409
    Clone ID Related Accession (Same CDS sequence) XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
    Accession Version XM_017009881.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865370.1
    CDS1502..7369
    Translation

    Target ORF information:

    RefSeq Version XM_017009881.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72409
    Clone ID Related Accession (Same CDS sequence) XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
    Accession Version XM_011514141.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512443.1
    CDS846..6713
    Translation

    Target ORF information:

    RefSeq Version XM_011514141.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72409
    Clone ID Related Accession (Same CDS sequence) XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
    Accession Version XM_017009881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865370.1
    CDS1908..7775
    Translation

    Target ORF information:

    RefSeq Version XM_017009881.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
    TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA
    AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA
    CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA
    AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA
    GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA
    GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT
    ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT
    GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC
    GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC
    CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA
    TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG
    GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA
    TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT
    GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA
    GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG
    AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT
    GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT
    GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC
    AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA
    TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA
    TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC
    TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT
    GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA
    CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT
    CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72410
    Clone ID Related Accession (Same CDS sequence) XM_011514142.1
    Accession Version XM_011514142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT
    GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512444.1
    CDS217..5661
    Translation

    Target ORF information:

    RefSeq Version XM_011514142.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT
    GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96093
    Clone ID Related Accession (Same CDS sequence) XM_017009880.2 , XM_017009880.1
    Accession Version XM_017009880.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865369.1
    CDS632..6739
    Translation

    Target ORF information:

    RefSeq Version XM_017009880.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96093
    Clone ID Related Accession (Same CDS sequence) XM_017009880.2 , XM_017009880.1
    Accession Version XM_017009880.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6108bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016865369.1
    CDS224..6331
    Translation

    Target ORF information:

    RefSeq Version XM_017009880.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT
    GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA
    CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT
    GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT
    ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA
    GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT
    ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA
    TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA
    TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT
    AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC
    AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT
    GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA
    GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA
    GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA
    GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA
    AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG
    AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA
    GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA
    CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA
    ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC
    AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG
    ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT
    CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG
    GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC
    CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC
    ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC
    CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA
    GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT
    GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA
    CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT
    GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA
    GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA
    AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA
    AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG
    GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT
    GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC
    ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA
    ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT
    GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA
    AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA
    GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT
    ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA
    GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT
    CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG
    AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC
    TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC
    TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA
    ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC
    CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA
    AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC
    CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT
    ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG
    ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC
    GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG
    AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA
    GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT
    AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC
    AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT
    CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG
    TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA
    GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT
    ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC
    ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC
    CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA
    GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT
    GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA
    GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC
    AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC
    ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA
    ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA
    GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT
    TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72403
    Clone ID Related Accession (Same CDS sequence) XM_011514135.2 , XM_011514135.3
    Accession Version XM_011514135.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA
    AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512437.1
    CDS222..6515
    Translation

    Target ORF information:

    RefSeq Version XM_011514135.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514135.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA
    AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72403
    Clone ID Related Accession (Same CDS sequence) XM_011514135.2 , XM_011514135.3
    Accession Version XM_011514135.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6294bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA
    AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512437.1
    CDS104..6397
    Translation

    Target ORF information:

    RefSeq Version XM_011514135.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514135.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA
    AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72404
    Clone ID Related Accession (Same CDS sequence) XM_011514136.2 , XM_011514136.3
    Accession Version XM_011514136.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA
    GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512438.1
    CDS221..6511
    Translation

    Target ORF information:

    RefSeq Version XM_011514136.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514136.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA
    GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72404
    Clone ID Related Accession (Same CDS sequence) XM_011514136.2 , XM_011514136.3
    Accession Version XM_011514136.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA
    GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512438.1
    CDS104..6394
    Translation

    Target ORF information:

    RefSeq Version XM_011514136.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514136.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA
    GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA
    GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT
    GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC
    ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA
    TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT
    GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT
    GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA
    CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC
    CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC
    CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC
    AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA
    GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA
    AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT
    TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA
    GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA
    CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG
    AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA
    ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT
    TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT
    TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA
    TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT
    ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA
    GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA
    CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG
    GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT
    CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC
    CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA
    AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG
    TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT
    AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT
    ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG
    ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA
    TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA
    GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA
    AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA
    CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT
    CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA
    AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC
    TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA
    ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG
    CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG
    GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA
    GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG
    CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT
    GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC
    AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA
    AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG
    GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG
    GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA
    GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA
    GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG
    GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT
    TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA
    GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72405
    Clone ID Related Accession (Same CDS sequence) XM_011514137.2 , XM_011514137.3
    Accession Version XM_011514137.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512439.1
    CDS221..6499
    Translation

    Target ORF information:

    RefSeq Version XM_011514137.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72405
    Clone ID Related Accession (Same CDS sequence) XM_011514137.2 , XM_011514137.3
    Accession Version XM_011514137.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6279bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512439.1
    CDS104..6382
    Translation

    Target ORF information:

    RefSeq Version XM_011514137.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514137.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA
    CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG
    CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT
    GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT
    GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT
    AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT
    GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT
    GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT
    AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA
    GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT
    TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA
    GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA
    AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC
    CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG
    AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA
    TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG
    ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72406
    Clone ID Related Accession (Same CDS sequence) XM_011514138.2 , XM_011514138.3
    Accession Version XM_011514138.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT
    CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA
    CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG
    GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT
    GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT
    GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA
    GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC
    ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC
    AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC
    ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA
    CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG
    AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT
    TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT
    CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT
    ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG
    ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT
    ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC
    TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC
    CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT
    GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA
    AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC
    TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA
    ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT
    GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG
    AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA
    AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA
    CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC
    ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA
    CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA
    GGAAAACAGT GTGCAAGATG GAGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512440.1
    CDS222..6491
    Translation

    Target ORF information:

    RefSeq Version XM_011514138.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514138.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT
    CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA
    CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG
    GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT
    GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT
    GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA
    GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC
    ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC
    AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC
    ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA
    CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG
    AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT
    TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT
    CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT
    ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG
    ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT
    ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC
    TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC
    CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT
    GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA
    AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC
    TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA
    ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT
    GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG
    AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA
    AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA
    CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC
    ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA
    CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA
    GGAAAACAGT GTGCAAGATG GAGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72406
    Clone ID Related Accession (Same CDS sequence) XM_011514138.2 , XM_011514138.3
    Accession Version XM_011514138.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT
    CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA
    CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG
    GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT
    GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT
    GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA
    GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC
    ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC
    AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC
    ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA
    CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG
    AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT
    TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT
    CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT
    ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG
    ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT
    ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC
    TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC
    CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT
    GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA
    AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC
    TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA
    ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT
    GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG
    AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA
    AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA
    CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC
    ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA
    CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA
    GGAAAACAGT GTGCAAGATG GAGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512440.1
    CDS104..6373
    Translation

    Target ORF information:

    RefSeq Version XM_011514138.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514138.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA
    GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA
    CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA
    TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA
    CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT
    CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG
    GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA
    GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG
    CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG
    AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC
    CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT
    ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT
    CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA
    TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC
    GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC
    AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA
    AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC
    ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA
    AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA
    CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA
    TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA
    AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT
    TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC
    CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG
    CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT
    CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA
    CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG
    GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT
    GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT
    GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA
    GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC
    ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC
    AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC
    ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA
    CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG
    AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT
    TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT
    CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT
    ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG
    ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT
    ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC
    TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC
    CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT
    GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA
    AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC
    TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA
    ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT
    GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG
    AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA
    AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA
    CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC
    ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC
    TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA
    CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA
    GGAAAACAGT GTGCAAGATG GAGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72407
    Clone ID Related Accession (Same CDS sequence) XM_011514139.2 , XM_011514139.3
    Accession Version XM_011514139.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512441.1
    CDS221..6439
    Translation

    Target ORF information:

    RefSeq Version XM_011514139.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514139.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72407
    Clone ID Related Accession (Same CDS sequence) XM_011514139.2 , XM_011514139.3
    Accession Version XM_011514139.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512441.1
    CDS104..6322
    Translation

    Target ORF information:

    RefSeq Version XM_011514139.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514139.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT
    AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA
    GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA
    TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA
    AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT
    GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG
    GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA
    GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG
    TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT
    GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA
    GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG
    CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA
    GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT
    TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA
    GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT
    CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC
    ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT
    GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA
    CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT
    ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG
    GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT
    GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT
    AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA
    AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA
    TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT
    GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA
    GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT
    AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC
    ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT
    CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA
    GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT
    CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA
    TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT
    GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT
    GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC
    CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC
    TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA
    ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT
    GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA
    AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT
    GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA
    AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA
    AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT
    AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG
    TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA
    CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA
    AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG
    GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA
    CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA
    GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA
    ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA
    AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC
    AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG
    CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA
    AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC
    TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA
    CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA
    ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG
    GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT
    CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG
    GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC
    AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG
    AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA
    GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC
    ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG
    AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT
    GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA
    GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT
    GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT
    GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA
    GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG
    AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA
    GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA
    TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA
    CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG
    AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT
    TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG
    TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC
    AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT
    GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG
    ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG
    TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG
    GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC
    ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA
    ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT
    TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG
    CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG
    GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC
    TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA
    GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT
    GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA
    AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA
    GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC
    GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG
    TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA
    CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu72408
    Clone ID Related Accession (Same CDS sequence) XM_011514140.2
    Accession Version XM_011514140.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG
    CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA
    AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT
    GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG
    GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA
    AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA
    TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT
    ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT
    GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT
    TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT
    CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT
    CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT
    CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG
    GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT
    GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA
    CCAGTTCCTG AGGAAATGCC TTTGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC
    TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGACCAGCA TACTGTGCTT
    GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC GGATCACAAG
    CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT
    GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG
    GACATTTGTG ATGCCCGGAA GGAAGAGGCG GAGCAGGAGC GGCTTGACAT CATTAATGAG
    AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA
    GAGCTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA
    AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCGAA TCCCTTTGGT CCAACTGGAT
    AGTAAAGACA ATTCTGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT
    CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGGGCAA GATGGATAAC
    TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG
    CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG
    GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA
    AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT
    AAATCACCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA
    ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA
    TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT
    CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT
    GAGAGGTATT TGAATGAAAT GGCCAGTACA GAAAAATTAA CTGACGTAGC TCGCTATCAC
    ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT
    AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCATCAA TACGTCCTCC ACCTGTAGAA
    AAGGAAGAAG ATGGTACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA
    GATATGGCAC CTAAAGGAGA CAGGAAGTGG CTTCATGGAT TACAGGCAGC CCTCCAGAAC
    TTTCTCAAGG GATATCCTGC AATTGTTCGG CAACTGCACT CAGCAGGCAG AGGCAAAAGT
    GACACCAGTC AAAAGAAACC CAAAGAACTT CTGGAGTCCC TTCTCCAAGC TGACATTGTT
    AAATTTGCCC ACTTCTTGTT GGATGTCATG AATGTCCTTA GCATTCTGTC CTGTGTCAAT
    AAGAATAGAA ATTCCTCAAT TGCTGACATA TTTGCCACTT TAGAGTCAAT GCTGGAAATG
    CTCCAAATGT ATCAAACAAG ACTAGGGCCA AAAGAACGCA TGGTGGATTC AGCCACACAC
    TTTCATGGTA ACTGCCTCAG AGGGAAAGAA AACATTTCTG CTGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGACT TCAAGGCTGT TTCAGAGATG CCAGCCAAAA TGTGGTGAGG
    GCTACCGTGA TTGGAAGTTT TAAATTGTGG CCCACAAAGA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTAT CCATCCTGAC TGCACATTAT GAACCAGTAT TAGAAGCTGC CAATGTGAAA
    CTTAGTGAAG TGGATACTGA ATGGAGCATG TTGAAATTAG AGATTTATGC CAGATTTCAG
    AACATTCGAA AATTGACCTG GGATTTTGTG AATTCCATTT ACTTACACAA GTATCCAAAT
    ATCCTGACAC TAGTCGACCT AGTGTTGACC CTTCCTGCAA GTTCAGCTGA AGCAGAACAT
    GGCTTTAGTC AGATGAAATG GACCAAGTCA CATATGCATG CAAAAATCAA GGCTGAAAGC
    ATAATAGGAA ATAAAGCATT TACTGACATT CTGATCGATT TGGTGACCCT GAACCTTGGC
    ACAAACAACT TTCCTAGTAA TTGGATGCAC CTTACCCAAC CTGAATTACA GGAATTAACA
    TCTTTATTAA CAGTCAACTC CGAGTTCGTG GACTGGCGGA AGTTCCTGTT AGTAACCTCA
    ATGCCTTGGC CCATTCCCTT GGAGGAGGAG CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT
    GTGGATAAGG AGCAGTTGGG CACCATCACT TTTGAGCAGT ATATGCAGGC TGGTCTGTGG
    TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT TCCATTTAAT
    AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGCTATTTG CTGACTATGA GAAGGATCCA
    CCCCAGCTTG ACTACACACA GATGCTGCTT TACTTTGCTT GCCACCCAGA CACCGTGGAA
    GGAGTCTACA GGGCCCTCAG TGTGGCTGTT GGAACTCATG TCTTCCAACA AGTCAAAGCT
    TCCATTCCAA GTGCAGAAAA GACCTCCTCA ACTGATGCAG GTCCAGCTGA GGAATTTCCT
    GAACCTGAGG AAAATGCTGC AAGAGAAGAA AGGAAATTAA AAGACGACAC GGAGAAAAGG
    GAACAGAAGG ATGAAGAAAT CCCTGAAAAT GCAAACAATG AAAAGATGTC CATGGAAACA
    CTACTCAAAG TGTTCAAAGG GGGAAGTGAA GCACAGGACT CCAATAGATT TGCCAGCCAC
    CTAAAGATAG AGAACATTTA TGCAGAGGGC TTCATAAAAA CATTTCAAGA CCTAGGTGCC
    AAGAACCTGG AGCCAATTGA AGTCGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAGTTTCCT GTACACATCA TCTATGTAGT GGAAATGATA
    GTGTGTGCAA AAGGTTGGGC ACCACTTCCA TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011512442.1
    CDS127..6249
    Translation

    Target ORF information:

    RefSeq Version XM_011514140.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011514140.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG
    TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT
    AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT
    GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG
    TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG
    CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC
    TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT
    CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG
    AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG
    GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC
    ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC
    CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG
    CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA
    CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT
    GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA
    GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT
    GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG
    TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT
    GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC
    TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA
    GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC
    AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA
    TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG
    TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT
    GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT
    GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG
    CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC
    TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA
    AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT
    GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG
    GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA
    AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA
    TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT
    ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT
    GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT
    ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT
    TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT
    CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA
    GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT
    CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT
    CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG
    GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA
    TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT
    GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA
    CCAGTTCCTG AGGAAATGCC TTTGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC
    TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGACCAGCA TACTGTGCTT
    GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC GGATCACAAG
    CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT
    GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG
    GACATTTGTG ATGCCCGGAA GGAAGAGGCG GAGCAGGAGC GGCTTGACAT CATTAATGAG
    AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA
    GAGCTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA
    AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCGAA TCCCTTTGGT CCAACTGGAT
    AGTAAAGACA ATTCTGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT
    CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGGGCAA GATGGATAAC
    TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG
    CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG
    GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA
    AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT
    AAATCACCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA
    ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA
    TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT
    CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT
    GAGAGGTATT TGAATGAAAT GGCCAGTACA GAAAAATTAA CTGACGTAGC TCGCTATCAC
    ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT
    AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCATCAA TACGTCCTCC ACCTGTAGAA
    AAGGAAGAAG ATGGTACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA
    GATATGGCAC CTAAAGGAGA CAGGAAGTGG CTTCATGGAT TACAGGCAGC CCTCCAGAAC
    TTTCTCAAGG GATATCCTGC AATTGTTCGG CAACTGCACT CAGCAGGCAG AGGCAAAAGT
    GACACCAGTC AAAAGAAACC CAAAGAACTT CTGGAGTCCC TTCTCCAAGC TGACATTGTT
    AAATTTGCCC ACTTCTTGTT GGATGTCATG AATGTCCTTA GCATTCTGTC CTGTGTCAAT
    AAGAATAGAA ATTCCTCAAT TGCTGACATA TTTGCCACTT TAGAGTCAAT GCTGGAAATG
    CTCCAAATGT ATCAAACAAG ACTAGGGCCA AAAGAACGCA TGGTGGATTC AGCCACACAC
    TTTCATGGTA ACTGCCTCAG AGGGAAAGAA AACATTTCTG CTGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGACT TCAAGGCTGT TTCAGAGATG CCAGCCAAAA TGTGGTGAGG
    GCTACCGTGA TTGGAAGTTT TAAATTGTGG CCCACAAAGA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTAT CCATCCTGAC TGCACATTAT GAACCAGTAT TAGAAGCTGC CAATGTGAAA
    CTTAGTGAAG TGGATACTGA ATGGAGCATG TTGAAATTAG AGATTTATGC CAGATTTCAG
    AACATTCGAA AATTGACCTG GGATTTTGTG AATTCCATTT ACTTACACAA GTATCCAAAT
    ATCCTGACAC TAGTCGACCT AGTGTTGACC CTTCCTGCAA GTTCAGCTGA AGCAGAACAT
    GGCTTTAGTC AGATGAAATG GACCAAGTCA CATATGCATG CAAAAATCAA GGCTGAAAGC
    ATAATAGGAA ATAAAGCATT TACTGACATT CTGATCGATT TGGTGACCCT GAACCTTGGC
    ACAAACAACT TTCCTAGTAA TTGGATGCAC CTTACCCAAC CTGAATTACA GGAATTAACA
    TCTTTATTAA CAGTCAACTC CGAGTTCGTG GACTGGCGGA AGTTCCTGTT AGTAACCTCA
    ATGCCTTGGC CCATTCCCTT GGAGGAGGAG CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT
    GTGGATAAGG AGCAGTTGGG CACCATCACT TTTGAGCAGT ATATGCAGGC TGGTCTGTGG
    TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT TCCATTTAAT
    AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGCTATTTG CTGACTATGA GAAGGATCCA
    CCCCAGCTTG ACTACACACA GATGCTGCTT TACTTTGCTT GCCACCCAGA CACCGTGGAA
    GGAGTCTACA GGGCCCTCAG TGTGGCTGTT GGAACTCATG TCTTCCAACA AGTCAAAGCT
    TCCATTCCAA GTGCAGAAAA GACCTCCTCA ACTGATGCAG GTCCAGCTGA GGAATTTCCT
    GAACCTGAGG AAAATGCTGC AAGAGAAGAA AGGAAATTAA AAGACGACAC GGAGAAAAGG
    GAACAGAAGG ATGAAGAAAT CCCTGAAAAT GCAAACAATG AAAAGATGTC CATGGAAACA
    CTACTCAAAG TGTTCAAAGG GGGAAGTGAA GCACAGGACT CCAATAGATT TGCCAGCCAC
    CTAAAGATAG AGAACATTTA TGCAGAGGGC TTCATAAAAA CATTTCAAGA CCTAGGTGCC
    AAGAACCTGG AGCCAATTGA AGTCGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG
    ATTTCAAATT ATTCAGACTA TAAGTTTCCT GTACACATCA TCTATGTAGT GGAAATGATA
    GTGTGTGCAA AAGGTTGGGC ACCACTTCCA TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.