DDX54 cDNA ORF clone, Homo sapiens(human)

The following DDX54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06329 NM_001111322.2
Latest version!
Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu04022 NM_024072.4
Latest version!
Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
View Old Accession Versions >>
OHu06329 NM_001111322.1 Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu04022 NM_024072.3 Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu06329
    Clone ID Related Accession (Same CDS sequence) NM_001111322.2 , NM_001111322.1
    Accession Version NM_001111322.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product ATP-dependent RNA helicase DDX54 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF478457.1 and BC005848.2. On Jun 2, 2019 this sequence version replaced NM_001111322.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478457.1, SRR7346977.1630778.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGCAGGTGC ATCCCGGCCC CACGCCCCAG GCACCCCTGC AGGCCGAGTC
    CGCCCGGAAC TCAAGACCAA GCAGCAGATC CTGAAGCAGC GGCGCCGGGC CCAGAAGCTG
    CACTTCCTGC AGCGTGGTGG CCTCAAGCAG CTCTCTGCCC GCAACCGCCG CCGCGTCCAG
    GAGCTGCAGC AGGGCGCCTT CGGCCGGGGT GCCCGCTCCA AGAAGGGCAA GATGCGGAAG
    AGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104792.1
    CDS28..2676
    Translation

    Target ORF information:

    RefSeq Version NM_001111322.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGCAGGTGC ATCCCGGCCC CACGCCCCAG GCACCCCTGC AGGCCGAGTC
    CGCCCGGAAC TCAAGACCAA GCAGCAGATC CTGAAGCAGC GGCGCCGGGC CCAGAAGCTG
    CACTTCCTGC AGCGTGGTGG CCTCAAGCAG CTCTCTGCCC GCAACCGCCG CCGCGTCCAG
    GAGCTGCAGC AGGGCGCCTT CGGCCGGGGT GCCCGCTCCA AGAAGGGCAA GATGCGGAAG
    AGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu04022
    Clone ID Related Accession (Same CDS sequence) NM_024072.3 , NM_024072.4
    Accession Version NM_024072.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Homo sapiens(human)
    Product ATP-dependent RNA helicase DDX54 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF478457.1 and BC005848.2. On Aug 10, 2004 this sequence version replaced NM_024072.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005848.2, SRR1660807.163053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGGTGCATC CCGGCCCCAC GCCCCAGGCA CCCCTGCAGG CCGAGTCCGC
    CCGGAACTCA AGACCAAGCA GCAGATCCTG AAGCAGCGGC GCCGGGCCCA GAAGCTGCAC
    TTCCTGCAGC GTGGTGGCCT CAAGCAGCTC TCTGCCCGCA ACCGCCGCCG CGTCCAGGAG
    CTGCAGCAGG GCGCCTTCGG CCGGGGTGCC CGCTCCAAGA AGGGCAAGAT GCGGAAGAGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_076977.3
    CDS29..2674
    Translation

    Target ORF information:

    RefSeq Version NM_024072.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024072.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGGTGCATC CCGGCCCCAC GCCCCAGGCA CCCCTGCAGG CCGAGTCCGC
    CCGGAACTCA AGACCAAGCA GCAGATCCTG AAGCAGCGGC GCCGGGCCCA GAAGCTGCAC
    TTCCTGCAGC GTGGTGGCCT CAAGCAGCTC TCTGCCCGCA ACCGCCGCCG CGTCCAGGAG
    CTGCAGCAGG GCGCCTTCGG CCGGGGTGCC CGCTCCAAGA AGGGCAAGAT GCGGAAGAGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu04022
    Clone ID Related Accession (Same CDS sequence) NM_024072.3 , NM_024072.4
    Accession Version NM_024072.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2646bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product ATP-dependent RNA helicase DDX54 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF478457.1 and BC005848.2. On Jun 2, 2019 this sequence version replaced NM_024072.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005848.2, SRR1660807.163053.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGGTGCATC CCGGCCCCAC GCCCCAGGCA CCCCTGCAGG CCGAGTCCGC
    CCGGAACTCA AGACCAAGCA GCAGATCCTG AAGCAGCGGC GCCGGGCCCA GAAGCTGCAC
    TTCCTGCAGC GTGGTGGCCT CAAGCAGCTC TCTGCCCGCA ACCGCCGCCG CGTCCAGGAG
    CTGCAGCAGG GCGCCTTCGG CCGGGGTGCC CGCTCCAAGA AGGGCAAGAT GCGGAAGAGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_076977.3
    CDS28..2673
    Translation

    Target ORF information:

    RefSeq Version NM_024072.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024072.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGGTGCATC CCGGCCCCAC GCCCCAGGCA CCCCTGCAGG CCGAGTCCGC
    CCGGAACTCA AGACCAAGCA GCAGATCCTG AAGCAGCGGC GCCGGGCCCA GAAGCTGCAC
    TTCCTGCAGC GTGGTGGCCT CAAGCAGCTC TCTGCCCGCA ACCGCCGCCG CGTCCAGGAG
    CTGCAGCAGG GCGCCTTCGG CCGGGGTGCC CGCTCCAAGA AGGGCAAGAT GCGGAAGAGG
    ATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06329
    Clone ID Related Accession (Same CDS sequence) NM_001111322.2 , NM_001111322.1
    Accession Version NM_001111322.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product ATP-dependent RNA helicase DDX54 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF478457.1 and BC005848.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF478457.1, SRR1803611.171886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGCAGGTGC ATCCCGGCCC CACGCCCCAG GCACCCCTGC AGGCCGAGTC
    CGCCCGGAAC TCAAGACCAA GCAGCAGATC CTGAAGCAGC GGCGCCGGGC CCAGAAGCTG
    CACTTCCTGC AGCGTGGTGG CCTCAAGCAG CTCTCTGCCC GCAACCGCCG CCGCGTCCAG
    GAGCTGCAGC AGGGCGCCTT CGGCCGGGGT GCCCGCTCCA AGAAGGGCAA GATGCGGAAG
    AGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001104792.1
    CDS29..2677
    Translation

    Target ORF information:

    RefSeq Version NM_001111322.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DEAD-box helicase 54 (DDX54), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001111322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCG ACAAGGGCCC GGCGGCTGGA CCTCGGTCGC GAGCTGCCAT GGCCCAGTGG 
    AGGAAGAAGA AAGGGCTCCG GAAGCGCCGA GGCGCGGCCT CCCAGGCCCG CGGCAGCGAC
    TCGGAGGACG GCGAGTTTGA GATCCAGGCG GAAGATGACG CCCGGGCCCG GAAGCTGGGA
    CCTGGAAGAC CCCTGCCCAC CTTCCCCACC TCGGAATGCA CCTCGGATGT GGAGCCGGAC
    ACCCGGGAGA TGGTGCGTGC CCAGAACAAG AAGAAGAAGA AGTCTGGAGG CTTCCAGTCC
    ATGGGCCTGA GCTACCCGGT GTTCAAAGGC ATCATGAAGA AGGGGTACAA GGTGCCAACA
    CCCATCCAGA GGAAGACCAT CCCGGTGATC TTGGATGGCA AGGACGTGGT GGCCATGGCC
    CGGACGGGCA GTGGCAAGAC AGCCTGCTTC CTCCTCCCAA TGTTCGAGCG GCTCAAGACC
    CACAGTGCCC AGACCGGGGC CCGCGCCCTC ATCCTCTCGC CGACCCGAGA GCTGGCCCTG
    CAGACCCTGA AGTTCACTAA GGAGCTAGGC AAGTTCACTG GCCTCAAGAC TGCCCTGATC
    CTGGGTGGAG ACAGGATGGA AGACCAGTTT GCAGCCCTGC ACGAAAATCC CGACATAATT
    ATTGCCACGC CCGGACGGTT GGTGCATGTG GCTGTGGAAA TGAGCCTGAA GCTGCAGAGT
    GTGGAATACG TGGTGTTCGA TGAAGCTGAC CGGCTTTTTG AAATGGGTTT CGCAGAGCAG
    CTGCAGGAGA TCATCGCCCG CCTCCCCGGG GGCCACCAGA CGGTGCTGTT CTCCGCCACG
    CTGCCCAAAC TGCTGGTGGA ATTTGCCCGG GCTGGCCTCA CGGAGCCCGT GCTCATCCGG
    CTTGACGTGG ATACCAAGCT CAACGAGCAG CTGAAGACCT CCTTCTTCCT CGTGCGGGAG
    GACACCAAGG CTGCCGTGCT GCTCCACCTG CTGCACAACG TGGTGCGGCC CCAGGACCAG
    ACCGTGGTGT TTGTGGCCAC GAAGCACCAC GCCGAGTACC TCACTGAGCT GCTGACGACC
    CAGCGGGTGA GCTGCGCCCA CATCTACAGT GCCCTAGACC CGACAGCCCG CAAGATCAAT
    CTCGCCAAAT TCACGCTTGG CAAGTGCTCC ACTCTCATTG TGACTGACCT GGCCGCCCGA
    GGCCTGGACA TCCCGCTGCT GGACAATGTC ATCAACTACA GCTTCCCCGC CAAGGGCAAA
    CTCTTCCTGC ACCGCGTGGG CCGTGTGGCT CGGGCTGGCC GAAGTGGCAC AGCCTACTCC
    TTGGTGGCCC CTGATGAAAT CCCCTACCTG CTGGATCTGC ACCTGTTCCT GGGCCGCTCC
    CTCACCCTCG CCCGACCCCT CAAGGAGCCC TCAGGTGTGG CCGGTGTGGA TGGCATGCTG
    GGTCGGGTGC CACAGAGTGT GGTGGACGAG GAGGACAGTG GTCTGCAGAG CACCCTGGAG
    GCATCGCTGG AGCTACGGGG CCTGGCCCGC GTTGCTGATA ACGCCCAGCA GCAGTATGTG
    CGCTCACGCC CGGCGCCCTC GCCTGAGTCC ATCAAGAGGG CCAAGGAGAT GGACCTTGTG
    GGGCTGGGCC TGCACCCCCT CTTCAGCTCG CGTTTTGAGG AGGAGGAGCT GCAGCGGCTG
    AGGCTGGTGG ACAGCATAAA GAACTACCGC TCCCGGGCGA CTATCTTTGA GATCAACGCC
    TCCAGCCGAG ACCTGTGCAG CCAGGTGATG CGCGCCAAGC GGCAGAAGGA CCGCAAGGCC
    ATCGCCCGCT TCCAGCAGGG ACAGCAGGGG CGGCAGGAGC AGCAGGAGGG CCCAGTGGGC
    CCAGCCCCGA GCCGCCCAGC ACTGCAGGAG AAGCAGCCTG AGAAGGAGGA GGAGGAGGAG
    GCGGGAGAGA GTGTGGAGGA CATTTTCTCA GAGGTCGTGG GCCGGAAGCG GCAGCGGTCA
    GGACCCAACA GGGGAGCCAA GAGGCGGAGG GAGGAGGCCC GGCAGCGGGA CCAGGAATTC
    TACATCCCCT ACCGGCCCAA GGACTTTGAC AGCGAGCGGG GCCTGAGCAT CAGCGGGGAA
    GGGGGAGCCT TTGAGCAGCA GGCAGCTGGC GCTGTCCTGG ACTTGATGGG GGATGAAGCC
    CAGAACCTGA CGAGGGGCCG GCAGCAGCTC AAGTGGGACC GTAAGAAGAA GCGGTTTGTG
    GGACAGTCAG GACAGGAAGA CAAGAAGAAG ATTAAGACAG AGAGCGGCCG CTACATCAGC
    AGCTCCTACA AGCGAGACCT CTATCAGAAG TGGAAACAGA AACAGAAAAT TGATGATCGT
    GACTCGGACG AAGAAGGGGC ATCTGACCGG CGAGGCCCAG AGCGAAGAGG TGGGAAGCGA
    GACCGTGGCC AAGCAGGTGC ATCCCGGCCC CACGCCCCAG GCACCCCTGC AGGCCGAGTC
    CGCCCGGAAC TCAAGACCAA GCAGCAGATC CTGAAGCAGC GGCGCCGGGC CCAGAAGCTG
    CACTTCCTGC AGCGTGGTGG CCTCAAGCAG CTCTCTGCCC GCAACCGCCG CCGCGTCCAG
    GAGCTGCAGC AGGGCGCCTT CGGCCGGGGT GCCCGCTCCA AGAAGGGCAA GATGCGGAAG
    AGGATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.