MAPKAPK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAPKAPK3
Entrez Gene ID	7867
Full Name	mitogen-activated protein kinase-activated protein kinase 3
Synonyms	3PK,MAPKAP-K3,MAPKAP3,MAPKAPK-3,MDPT3,MK-3
General protein information	Preferred Names mitogen-activated protein kinase-activated protein kinase 3 Names MAP kinase-activated protein kinase 3 MAPK-activated protein kinase 3 MAPKAP kinase 3 chromosome 3p kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.2
Summary	This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011].
Disorder MIM:	602130
Disorder Html:	?Macular dystrophy, patterned, 3, 617111 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001243925.1	NP_001230854.1	MAP kinase-activated protein kinase 3
NM_001243925.2	NP_001230854.1	MAP kinase-activated protein kinase 3
NM_004635.4	NP_004626.1	MAP kinase-activated protein kinase 3
NM_001243926.1	NP_001230855.1	MAP kinase-activated protein kinase 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAPKAPK3	XP_516486.3
	MAPKAPK3	XP_001097318.1
Drosophila melanogaster (fruit fly)	MAPk-Ak2	NP_001188547.1
Mus musculus (house mouse)	Mapkapk3	NP_849238.1
Danio rerio (zebrafish)	mapkapk3	NP_001073548.1
Xenopus tropicalis (tropical clawed frog)	mapkapk3	NP_001072862.1
Bos taurus (cattle)	MAPKAPK3	NP_001029951.1
Gallus gallus (chicken)	MAPKAPK3	XP_414262.3
Canis lupus familiaris (dog)	MAPKAPK3	XP_541872.3
Rattus norvegicus (Norway rat)	Mapkapk3	NP_001012127.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011890	XP_320635.4
Caenorhabditis elegans (roundworm)	mak-2	NP_500005.1
Homo sapiens (human)	MAPKAPK3	NP_004626.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Martinique Crinkled Retinal Pigment Epitheliopathy: Clinical Stages and Pathophysiologic Insights.
Jean-Charles A, Merle H, Audo I, Desoudin C, Bocquet B, Baudoin C, Sidibe M, Mauget-Fa?sse M, Wolff B, Fichard A, Lenaers G, Sahel JA, Gaudric A, Cohen SY, Hamel CP, Meunier I
Ophthalmology123(10)2196-204(2016 Oct)

A dominant mutation in MAPKAPK3, an actor of p38 signaling pathway, causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.
Meunier I, Lenaers G, Bocquet B, Baudoin C, Piro-Megy C, Cubizolle A, Quil?s M, Jean-Charles A, Cohen SY, Merle H, Gaudric A, Labesse G, Manes G, P?quignot M, Cazevieille C, Dhaenens CM, Fichard A, Ronkina N, Arthur SJ, Gaestel M, Hamel CP
Human molecular genetics25(5)916-26(2016 Mar)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

MK3 modulation affects BMI1-dependent and independent cell cycle check-points.
Prickaerts P, Niessen HE, Dahlmans VE, Spaapen F, Salvaing J, Vanhove J, Geijselaers C, Bartels SJ, Partouns I, Neumann D, Speel EJ, Takihara Y, Wouters BG, Voncken JW
PloS one10(4)e0118840(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The natural history of MCRPE is in relation to the role of MAPKAPK3 in BM modeling, vascular endothelial growth factor activity, retinal pigment epithelial responses
Title: Martinique Crinkled Retinal Pigment Epitheliopathy: Clinical Stages and Pathophysiologic Insights.

A dominant mutation in MAPKAPK3 causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.
Title: A dominant mutation in MAPKAPK3, an actor of p38 signaling pathway, causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.

findings reveal MK2/MK3 as crucial stress-responsive kinases that promote autophagy through Beclin 1 S90 phosphorylation
Title: The stress-responsive kinases MAPKAPK2/MAPKAPK3 activate starvation-induced autophagy through Beclin 1 phosphorylation.

MK3 modulation affects BMI1-dependent and independent cell cycle check-points
Title: MK3 modulation affects BMI1-dependent and independent cell cycle check-points.

Hepatitis C virus core protein interacted with MAPKAPK3 through amino acid residues 41 to 75 of core and the N-terminal half of kinase domain of MAPKAPK3.
Title: Modulation of mitogen-activated protein kinase-activated protein kinase 3 by hepatitis C virus core protein.

The following MAPKAPK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAPKAPK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07888	NM_001243925.2 Latest version!	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07888	NM_004635.4 Latest version!	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07888	NM_001243926.1 Latest version!	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu07888	NM_001243925.1	Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAPKAPK3 ( NM_001243925.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230854.1 Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07888
Clone ID Related Accession (Same CDS sequence)	NM_001243925.1 , NM_001243925.2 , NM_004635.4 , NM_001243926.1
Accession Version	NM_001243925.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	MAP kinase-activated protein kinase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848086.1, U09578.1, AC096920.2 and BE301676.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.211140.1, SRR1660809.207130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230854.1
CDS	164..1312
Translation	MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

Target ORF information:

RefSeq Version	NM_001243925.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243925.1

ORF Insert Sequence:

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPKAPK3 ( NM_001243925.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230854.1 Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07888
Clone ID Related Accession (Same CDS sequence)	NM_001243925.1 , NM_001243925.2 , NM_004635.4 , NM_001243926.1
Accession Version	NM_001243925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	MAP kinase-activated protein kinase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848086.1, U09578.1, AC096920.2 and BE301676.1. On Nov 23, 2018 this sequence version replaced NM_001243925.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.211140.1, SRR1660807.98658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000621469.5/ ENSP00000478922.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230854.1
CDS	144..1292
Translation	MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

Target ORF information:

RefSeq Version	NM_001243925.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243925.2

ORF Insert Sequence:

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPKAPK3 ( NM_004635.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004626.1 Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07888
Clone ID Related Accession (Same CDS sequence)	NM_001243925.1 , NM_001243925.2 , NM_004635.4 , NM_001243926.1
Accession Version	NM_004635.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	MAP kinase-activated protein kinase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848086.1, BC001662.2, AC096920.2 and BE301676.1. On Sep 2, 2011 this sequence version replaced NM_004635.3. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.167488.1, SRR1660805.239672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004626.1
CDS	149..1297
Translation	MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

Target ORF information:

RefSeq Version	NM_004635.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004635.4

ORF Insert Sequence:

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAPKAPK3 ( NM_001243926.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230855.1 Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 1, mRNA.

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CloneID	OHu07888
Clone ID Related Accession (Same CDS sequence)	NM_001243925.1 , NM_001243925.2 , NM_004635.4 , NM_001243926.1
Accession Version	NM_001243926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	MAP kinase-activated protein kinase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB450001.1, AC096920.2, BC068497.1 and BE301676.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230855.1
CDS	643..1791
Translation	MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

Target ORF information:

RefSeq Version	NM_001243926.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MAPK activated protein kinase 3 (MAPKAPK3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243926.1

ORF Insert Sequence:

ATGGATGGTG AAACAGCAGA GGAGCAGGGG GGCCCTGTGC CCCCGCCAGT TGCACCCGGC 
GGACCCGGCT TGGGCGGTGC TCCGGGGGGG CGGCGGGAGC CCAAGAAGTA CGCAGTGACC 
GACGACTACC AGTTGTCCAA GCAGGTGCTG GGCCTGGGTG TGAACGGCAA AGTGCTGGAG 
TGCTTCCATC GGCGCACTGG ACAGAAGTGT GCCCTGAAGC TCCTGTATGA CAGCCCCAAG 
GCCCGGCAGG AGGTAGACCA TCACTGGCAG GCTTCTGGCG GCCCCCATAT TGTCTGCATC 
CTGGATGTGT ATGAGAACAT GCACCATGGC AAGCGCTGTC TCCTCATCAT CATGGAATGC 
ATGGAAGGTG GTGAGTTGTT CAGCAGGATT CAGGAGCGTG GCGACCAGGC TTTCACTGAG 
AGAGAAGCTG CAGAGATAAT GCGGGATATT GGCACTGCCA TCCAGTTTCT GCACAGCCAT 
AACATTGCCC ACCGAGATGT CAAGCCTGAA AACCTACTCT ACACATCTAA GGAGAAAGAC 
GCAGTGCTTA AGCTCACCGA TTTTGGCTTT GCTAAGGAGA CCACCCAAAA TGCCCTGCAG 
ACACCCTGCT ATACTCCCTA TTATGTGGCC CCTGAGGTCC TGGGTCCAGA GAAGTATGAC 
AAGTCATGTG ACATGTGGTC CCTGGGTGTC ATCATGTACA TCCTCCTTTG TGGCTTCCCA 
CCCTTCTACT CCAACACGGG CCAGGCCATC TCCCCGGGGA TGAAGAGGAG GATTCGCCTG 
GGCCAGTACG GCTTCCCCAA TCCTGAGTGG TCAGAAGTCT CTGAGGATGC CAAGCAGCTG 
ATCCGCCTCC TGTTGAAGAC AGACCCCACA GAGAGGCTGA CCATCACTCA GTTCATGAAC 
CACCCCTGGA TCAACCAATC GATGGTAGTG CCACAGACCC CACTCCACAC GGCCCGAGTG 
CTGCAGGAGG ACAAAGACCA CTGGGACGAA GTCAAGGAGG AGATGACCAG TGCCTTGGCC 
ACTATGCGGG TAGACTACGA CCAGGTGAAG ATCAAGGACC TGAAGACCTC TAACAACCGG 
CTCCTCAACA AGAGGAGAAA AAAGCAGGCA GGCAGCTCCT CTGCCTCACA GGGCTGCAAC 
AACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Martinique Crinkled Retinal Pigment Epitheliopathy: Clinical Stages and Pathophysiologic Insights.
Ophthalmology123(10)2196-204(2016 Oct)
Jean-Charles A,Merle H,Audo I,Desoudin C,Bocquet B,Baudoin C,Sidibe M,Mauget-Fa?sse M,Wolff B,Fichard A,Lenaers G,Sahel JA,Gaudric A,Cohen SY,Hamel CP,Meunier I

A dominant mutation in MAPKAPK3, an actor of p38 signaling pathway, causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.
Human molecular genetics25(5)916-26(2016 Mar)
Meunier I,Lenaers G,Bocquet B,Baudoin C,Piro-Megy C,Cubizolle A,Quil?s M,Jean-Charles A,Cohen SY,Merle H,Gaudric A,Labesse G,Manes G,P?quignot M,Cazevieille C,Dhaenens CM,Fichard A,Ronkina N,Arthur SJ,Gaestel M,Hamel CP

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

MK3 modulation affects BMI1-dependent and independent cell cycle check-points.
PloS one10(4)e0118840(2015)
Prickaerts P,Niessen HE,Dahlmans VE,Spaapen F,Salvaing J,Vanhove J,Geijselaers C,Bartels SJ,Partouns I,Neumann D,Speel EJ,Takihara Y,Wouters BG,Voncken JW

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MAPKAPK3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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