WT1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_024424.4	NP_077742.3	Wilms tumor protein isoform B
NM_024424.5	NP_077742.3	Wilms tumor protein isoform B
NM_001367854.1	NP_001354783.1	Wilms tumor protein isoform G
NM_024424.3	NP_077742.2	Wilms tumor protein isoform B
NM_000378.4	NP_000369.3	Wilms tumor protein isoform A
NM_000378.6	NP_000369.4	Wilms tumor protein isoform A
NM_001198551.1	NP_001185480.1	Wilms tumor protein isoform E
NM_001198552.1	NP_001185481.1	Wilms tumor protein isoform F
NM_024426.4	NP_077744.3	Wilms tumor protein isoform D
NM_000378.5	NP_000369.4	Wilms tumor protein isoform A
NM_024426.6	NP_077744.4	Wilms tumor protein isoform D
NM_024426.5	NP_077744.4	Wilms tumor protein isoform D
NM_001198552.2	NP_001185481.1	Wilms tumor protein isoform F

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27056	NM_000378.4 Latest version!	Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$209.50-$293.30 $419.00
OHu27290	NM_001198552.2 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu27316	NM_001198551.1 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu27351	NM_024426.4 Latest version!	Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$214.50-$300.30 $429.00
OHu27419	NM_024424.3 Latest version!	Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$214.50-$300.30 $429.00
OHu107770	NM_001367854.1 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu107650	NM_024424.5 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107651	NM_024426.6 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107652	NM_000378.6 Latest version!	Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu107650	NM_024424.4	Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107652	NM_000378.5	Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu107651	NM_024426.5	Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu27290	NM_001198552.1	Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

WT1 ( NM_000378.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000369.3 Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu27056
Clone ID Related Accession (Same CDS sequence)	NM_000378.4
Accession Version	NM_000378.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced NM_000378.3. Transcript Variant: This variant (A) lacks an alternate in-frame exon and uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform A), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA 
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC 
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT 
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC 
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT 
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG 
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG 
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG 
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000369.3
CDS	191..1684
Translation	LQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_000378.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Wilms tumor 1 (WT1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000378.4

ORF Insert Sequence:

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG CCACAGCACA 
GGGTACGAGA GCGATAACCA CACAACGCCC ATCCTCTGCG GAGCCCAATA CAGAATACAC 
ACGCACGGTG TCTTCAGAGG CATTCAGGAT GTGCGACGTG TGCCTGGAGT AGCCCCGACT 
CTTGTACGGT CGGCATCTGA GACCAGTGAG AAACGCCCCT TCATGTGTGC TTACCCAGGC 
TGCAATAAGA GATATTTTAA GCTGTCCCAC TTACAGATGC ACAGCAGGAA GCACACTGGT 
GAGAAACCAT ACCAGTGTGA CTTCAAGGAC TGTGAACGAA GGTTTTCTCG TTCAGACCAG 
CTCAAAAGAC ACCAAAGGAG ACATACAGGT GTGAAACCAT TCCAGTGTAA AACTTGTCAG 
CGAAAGTTCT CCCGGTCCGA CCACCTGAAG ACCCACACCA GGACTCATAC AGGTGAAAAG 
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_001198552.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185481.1 Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OHu27290
Clone ID Related Accession (Same CDS sequence)	NM_001198552.1 , NM_001198552.2
Accession Version	NM_001198552.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, AK093168.1, M30393.1 and BC046461.1. Transcript Variant: This variant (F) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also lacks an alternate, in-frame exon compared to variant D. The encoded isoform (F) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093168.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC 
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA 
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC 
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA 
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT 
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185481.1
CDS	279..1145
Translation	MEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_001198552.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198552.1

ORF Insert Sequence:

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC 
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA 
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC 
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA 
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT 
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_001198552.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185481.1 Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OHu27290
Clone ID Related Accession (Same CDS sequence)	NM_001198552.1 , NM_001198552.2
Accession Version	NM_001198552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, AK093168.1, M30393.1 and BC046461.1. On Jun 2, 2019 this sequence version replaced NM_001198552.1. Transcript Variant: This variant (F) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also lacks an alternate, in-frame exon compared to variant D. The encoded isoform (F) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK093168.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC 
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA 
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC 
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA 
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT 
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185481.1
CDS	275..1141
Translation	MEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_001198552.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198552.2

ORF Insert Sequence:

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGCCACAG CACAGGGTAC GAGAGCGATA ACCACACAAC GCCCATCCTC 
TGCGGAGCCC AATACAGAAT ACACACGCAC GGTGTCTTCA GAGGCATTCA GGATGTGCGA 
CGTGTGCCTG GAGTAGCCCC GACTCTTGTA CGGTCGGCAT CTGAGACCAG TGAGAAACGC 
CCCTTCATGT GTGCTTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCA GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGTGAA 
CGAAGGTTTT CTCGTTCAGA CCAGCTCAAA AGACACCAAA GGAGACATAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTCTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCCAAGTTGT 
CAGAAAAAGT TTGCCCGGTC AGATGAATTA GTCCGCCATC ACAACATGCA TCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_001198551.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185480.1 Homo sapiens WT1 transcription factor (WT1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OHu27316
Clone ID Related Accession (Same CDS sequence)	NM_001198551.1
Accession Version	NM_001198551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG718348.1, BC032861.2, AK093168.1, M30393.1 and BC046461.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (E) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant D. This variant also uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. The encoded isoform (E) has a distinct N-terminus and is shorter than isoform D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032861.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG 
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185480.1
CDS	279..1187
Translation	MEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_001198551.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198551.1

ORF Insert Sequence:

ATGGAGAAGG GTTACAGCAC GGTCACCTTC GACGGGACGC CCAGCTACGG TCACACGCCC 
TCGCACCATG CGGCGCAGTT CCCCAACCAC TCATTCAAGC ATGAGGATCC CATGGGCCAG 
CAGGGCTCGC TGGGTGAGCA GCAGTACTCG GTGCCGCCCC CGGTCTATGG CTGCCACACC 
CCCACCGACA GCTGCACCGG CAGCCAGGCT TTGCTGCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AAATGACATC CCAGCTTGAA TGCATGACCT GGAATCAGAT GAACTTAGGA 
GCCACCTTAA AGGGAGTTGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG 
AGCAACCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024426.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077744.3 Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OHu27351
Clone ID Related Accession (Same CDS sequence)	NM_024426.4
Accession Version	NM_024426.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced NM_024426.3. Transcript Variant: This variant (D) represents the longest transcript and encodes the longest isoform (D). It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG 
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT 
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG 
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC 
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG 
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077744.3
CDS	191..1744
Translation	LQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024426.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Wilms tumor 1 (WT1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024426.4

ORF Insert Sequence:

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG 
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT 
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG 
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC 
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTAAAAC AAGTGAAAAG 
CCCTTCAGCT GTCGGTGGCC AAGTTGTCAG AAAAAGTTTG CCCGGTCAGA TGAATTAGTC 
CGCCATCACA ACATGCATCA GAGAAACATG ACCAAACTCC AGCTGGCGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024424.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_077742.2 Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu27419
Clone ID Related Accession (Same CDS sequence)	NM_024424.3
Accession Version	NM_024424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Oct 23, 2010 this sequence version replaced NM_024424.2. Transcript Variant: This variant (B) uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform B), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG 
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT 
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG 
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC 
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC 
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC 
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077742.2
CDS	191..1735
Translation	LQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024424.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Wilms tumor 1 (WT1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024424.3

ORF Insert Sequence:

CTGCAGGACC CGGCTTCCAC GTGTGTCCCG GAGCCGGCGT CTCAGCACAC GCTCCGCTCC 
GGGCCTGGGT GCCTACAGCA GCCAGAGCAG CAGGGAGTCC GGGACCCGGG CGGCATCTGG 
GCCAAGTTAG GCGCCGCCGA GGCCAGCGCT GAACGTCTCC AGGGCCGGAG GAGCCGCGGG 
GCGTCCGGGT CTGAGCCGCA GCAAATGGGC TCCGACGTGC GGGACCTGAA CGCGCTGCTG 
CCCGCCGTCC CCTCCCTGGG TGGCGGCGGC GGCTGTGCCC TGCCTGTGAG CGGCGCGGCG 
CAGTGGGCGC CGGTGCTGGA CTTTGCGCCC CCGGGCGCTT CGGCTTACGG GTCGTTGGGC 
GGCCCCGCGC CGCCACCGGC TCCGCCGCCA CCCCCGCCGC CGCCGCCTCA CTCCTTCATC 
AAACAGGAGC CGAGCTGGGG CGGCGCGGAG CCGCACGAGG AGCAGTGCCT GAGCGCCTTC 
ACTGTCCACT TTTCCGGCCA GTTCACTGGC ACAGCCGGAG CCTGTCGCTA CGGGCCCTTC 
GGTCCTCCTC CGCCCAGCCA GGCGTCATCC GGCCAGGCCA GGATGTTTCC TAACGCGCCC 
TACCTGCCCA GCTGCCTCGA GAGCCAGCCC GCTATTCGCA ATCAGGGTTA CAGCACGGTC 
ACCTTCGACG GGACGCCCAG CTACGGTCAC ACGCCCTCGC ACCATGCGGC GCAGTTCCCC 
AACCACTCAT TCAAGCATGA GGATCCCATG GGCCAGCAGG GCTCGCTGGG TGAGCAGCAG 
TACTCGGTGC CGCCCCCGGT CTATGGCTGC CACACCCCCA CCGACAGCTG CACCGGCAGC 
CAGGCTTTGC TGCTGAGGAC GCCCTACAGC AGTGACAATT TATACCAAAT GACATCCCAG 
CTTGAATGCA TGACCTGGAA TCAGATGAAC TTAGGAGCCA CCTTAAAGGG AGTTGCTGCT 
GGGAGCTCCA GCTCAGTGAA ATGGACAGAA GGGCAGAGCA ACCACAGCAC AGGGTACGAG 
AGCGATAACC ACACAACGCC CATCCTCTGC GGAGCCCAAT ACAGAATACA CACGCACGGT 
GTCTTCAGAG GCATTCAGGA TGTGCGACGT GTGCCTGGAG TAGCCCCGAC TCTTGTACGG 
TCGGCATCTG AGACCAGTGA GAAACGCCCC TTCATGTGTG CTTACCCAGG CTGCAATAAG 
AGATATTTTA AGCTGTCCCA CTTACAGATG CACAGCAGGA AGCACACTGG TGAGAAACCA 
TACCAGTGTG ACTTCAAGGA CTGTGAACGA AGGTTTTCTC GTTCAGACCA GCTCAAAAGA 
CACCAAAGGA GACATACAGG TGTGAAACCA TTCCAGTGTA AAACTTGTCA GCGAAAGTTC 
TCCCGGTCCG ACCACCTGAA GACCCACACC AGGACTCATA CAGGTGAAAA GCCCTTCAGC 
TGTCGGTGGC CAAGTTGTCA GAAAAAGTTT GCCCGGTCAG ATGAATTAGT CCGCCATCAC 
AACATGCATC AGAGAAACAT GACCAAACTC CAGCTGGCGC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_001367854.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354783.1 Homo sapiens WT1 transcription factor (WT1), transcript variant G, mRNA.

Price & Availability↑

CloneID	OHu107770
Clone ID Related Accession (Same CDS sequence)	NM_001367854.1
Accession Version	NM_001367854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049692.13. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.212982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155371, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGTGCTT ACCCAGGCTG CAATAAGAGA TATTTTAAGC TGTCCCACTT ACAGATGCAC 
AGCAGGAAGC ACACTGGTGA GAAACCATAC CAGTGTGACT TCAAGGACTG TGAACGAAGG 
TTTTCTCGTT CAGACCAGCT CAAAAGACAC CAAAGGAGAC ATACAGGTGT GAAACCATTC 
CAGTGTAAAA CTTGTCAGCG AAAGTTCTCC CGGTCCGACC ACCTGAAGAC CCACACCAGG 
ACTCATACAG GTAAAACAAG TGAAAAGCCC TTCAGCTGTC GGTGGCCAAG TTGTCAGAAA 
AAGTTTGCCC GGTCAGATGA ATTAGTCCGC CATCACAACA TGCATCAGAG AAACATGACC 
AAACTCCAGC TGGCGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354783.1
CDS	428..808
Translation	MCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_001367854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367854.1

ORF Insert Sequence:

ATGTGTGCTT ACCCAGGCTG CAATAAGAGA TATTTTAAGC TGTCCCACTT ACAGATGCAC 
AGCAGGAAGC ACACTGGTGA GAAACCATAC CAGTGTGACT TCAAGGACTG TGAACGAAGG 
TTTTCTCGTT CAGACCAGCT CAAAAGACAC CAAAGGAGAC ATACAGGTGT GAAACCATTC 
CAGTGTAAAA CTTGTCAGCG AAAGTTCTCC CGGTCCGACC ACCTGAAGAC CCACACCAGG 
ACTCATACAG GTAAAACAAG TGAAAAGCCC TTCAGCTGTC GGTGGCCAAG TTGTCAGAAA 
AAGTTTGCCC GGTCAGATGA ATTAGTCCGC CATCACAACA TGCATCAGAG AAACATGACC 
AAACTCCAGC TGGCGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024424.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077742.3 Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu107650
Clone ID Related Accession (Same CDS sequence)	NM_024424.4 , NM_024424.5
Accession Version	NM_024424.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Jan 13, 2018 this sequence version replaced NM_024424.3. Transcript Variant: This variant (B) uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform B), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
GAAAAGCCCT TCAGCTGTCG GTGGCCAAGT TGTCAGAAAA AGTTTGCCCG GTCAGATGAA 
TTAGTCCGCC ATCACAACAT GCATCAGAGA AACATGACCA AACTCCAGCT GGCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077742.3
CDS	180..1739
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024424.4
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024424.4

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
GAAAAGCCCT TCAGCTGTCG GTGGCCAAGT TGTCAGAAAA AGTTTGCCCG GTCAGATGAA 
TTAGTCCGCC ATCACAACAT GCATCAGAGA AACATGACCA AACTCCAGCT GGCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024424.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_077742.3 Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OHu107650
Clone ID Related Accession (Same CDS sequence)	NM_024424.4 , NM_024424.5
Accession Version	NM_024424.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, AK291736.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Jun 2, 2019 this sequence version replaced NM_024424.4. Transcript Variant: This variant (B) uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform B), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291736.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
GAAAAGCCCT TCAGCTGTCG GTGGCCAAGT TGTCAGAAAA AGTTTGCCCG GTCAGATGAA 
TTAGTCCGCC ATCACAACAT GCATCAGAGA AACATGACCA AACTCCAGCT GGCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077742.3
CDS	180..1739
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024424.5
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024424.5

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
GAAAAGCCCT TCAGCTGTCG GTGGCCAAGT TGTCAGAAAA AGTTTGCCCG GTCAGATGAA 
TTAGTCCGCC ATCACAACAT GCATCAGAGA AACATGACCA AACTCCAGCT GGCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024426.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_077744.4 Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OHu107651
Clone ID Related Accession (Same CDS sequence)	NM_024426.6 , NM_024426.5
Accession Version	NM_024426.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Jun 2, 2019 this sequence version replaced NM_024426.5. Transcript Variant: This variant (D) represents the longest transcript and encodes the longest isoform (D). It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51630.1, MH550345.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
AAAACAAGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077744.4
CDS	180..1748
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024426.6
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024426.6

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
AAAACAAGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_024426.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_077744.4 Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OHu107651
Clone ID Related Accession (Same CDS sequence)	NM_024426.6 , NM_024426.5
Accession Version	NM_024426.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, X51630.1, M30393.1, AK093168.1 and BC046461.1. On Jan 13, 2018 this sequence version replaced NM_024426.4. Transcript Variant: This variant (D) represents the longest transcript and encodes the longest isoform (D). It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X51630.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
AAAACAAGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077744.4
CDS	180..1748
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_024426.5
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024426.5

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGAGTTG CTGCTGGGAG CTCCAGCTCA GTGAAATGGA CAGAAGGGCA GAGCAACCAC 
AGCACAGGGT ACGAGAGCGA TAACCACACA ACGCCCATCC TCTGCGGAGC CCAATACAGA 
ATACACACGC ACGGTGTCTT CAGAGGCATT CAGGATGTGC GACGTGTGCC TGGAGTAGCC 
CCGACTCTTG TACGGTCGGC ATCTGAGACC AGTGAGAAAC GCCCCTTCAT GTGTGCTTAC 
CCAGGCTGCA ATAAGAGATA TTTTAAGCTG TCCCACTTAC AGATGCACAG CAGGAAGCAC 
ACTGGTGAGA AACCATACCA GTGTGACTTC AAGGACTGTG AACGAAGGTT TTCTCGTTCA 
GACCAGCTCA AAAGACACCA AAGGAGACAT ACAGGTGTGA AACCATTCCA GTGTAAAACT 
TGTCAGCGAA AGTTCTCCCG GTCCGACCAC CTGAAGACCC ACACCAGGAC TCATACAGGT 
AAAACAAGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_000378.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000369.4 Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu107652
Clone ID Related Accession (Same CDS sequence)	NM_000378.6 , NM_000378.5
Accession Version	NM_000378.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, M30393.1, X51630.1, AK093168.1 and BC046461.1. On Jun 2, 2019 this sequence version replaced NM_000378.5. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGCCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000369.4
CDS	180..1688
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_000378.6
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000378.6

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGCCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WT1 ( NM_000378.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000369.4 Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu107652
Clone ID Related Accession (Same CDS sequence)	NM_000378.6 , NM_000378.5
Accession Version	NM_000378.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	Wilms tumor protein isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB971668.1, M30393.1, X51630.1, AK093168.1 and BC046461.1. On Jan 13, 2018 this sequence version replaced NM_000378.4. Transcript Variant: This variant (A) lacks an alternate in-frame exon and uses an alternate, in-frame splice site in the 3' coding region, compared to variant D. This results in a shorter protein (isoform A), compared to isoform D. It initiates translation from a non-AUG (CUG) site, and also from a downstream, in-frame AUG. CCDS Note: The coding region has been updated to start at a non-AUG translation start codon that is supported by a publication and protein conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9361029, 8012395 non-AUG initiation codon :: PMID: 8621495 undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGCCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000369.4
CDS	180..1688
Translation	LDFLLLQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRGASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_000378.5
Organism	Homo sapiens(human)
Definition	Homo sapiens WT1 transcription factor (WT1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000378.5

ORF Insert Sequence:

CTGGACTTCC TCTTGCTGCA GGACCCGGCT TCCACGTGTG TCCCGGAGCC GGCGTCTCAG 
CACACGCTCC GCTCCGGGCC TGGGTGCCTA CAGCAGCCAG AGCAGCAGGG AGTCCGGGAC 
CCGGGCGGCA TCTGGGCCAA GTTAGGCGCC GCCGAGGCCA GCGCTGAACG TCTCCAGGGC 
CGGAGGAGCC GCGGGGCGTC CGGGTCTGAG CCGCAGCAAA TGGGCTCCGA CGTGCGGGAC 
CTGAACGCGC TGCTGCCCGC CGTCCCCTCC CTGGGTGGCG GCGGCGGCTG TGCCCTGCCT 
GTGAGCGGCG CGGCGCAGTG GGCGCCGGTG CTGGACTTTG CGCCCCCGGG CGCTTCGGCT 
TACGGGTCGT TGGGCGGCCC CGCGCCGCCA CCGGCTCCGC CGCCACCCCC GCCGCCGCCG 
CCTCACTCCT TCATCAAACA GGAGCCGAGC TGGGGCGGCG CGGAGCCGCA CGAGGAGCAG 
TGCCTGAGCG CCTTCACTGT CCACTTTTCC GGCCAGTTCA CTGGCACAGC CGGAGCCTGT 
CGCTACGGGC CCTTCGGTCC TCCTCCGCCC AGCCAGGCGT CATCCGGCCA GGCCAGGATG 
TTTCCTAACG CGCCCTACCT GCCCAGCTGC CTCGAGAGCC AGCCCGCTAT TCGCAATCAG 
GGTTACAGCA CGGTCACCTT CGACGGGACG CCCAGCTACG GTCACACGCC CTCGCACCAT 
GCGGCGCAGT TCCCCAACCA CTCATTCAAG CATGAGGATC CCATGGGCCA GCAGGGCTCG 
CTGGGTGAGC AGCAGTACTC GGTGCCGCCC CCGGTCTATG GCTGCCACAC CCCCACCGAC 
AGCTGCACCG GCAGCCAGGC TTTGCTGCTG AGGACGCCCT ACAGCAGTGA CAATTTATAC 
CAAATGACAT CCCAGCTTGA ATGCATGACC TGGAATCAGA TGAACTTAGG AGCCACCTTA 
AAGGGCCACA GCACAGGGTA CGAGAGCGAT AACCACACAA CGCCCATCCT CTGCGGAGCC 
CAATACAGAA TACACACGCA CGGTGTCTTC AGAGGCATTC AGGATGTGCG ACGTGTGCCT 
GGAGTAGCCC CGACTCTTGT ACGGTCGGCA TCTGAGACCA GTGAGAAACG CCCCTTCATG 
TGTGCTTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
AGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGTGA ACGAAGGTTT 
TCTCGTTCAG ACCAGCTCAA AAGACACCAA AGGAGACATA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTCTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCCAAGTT GTCAGAAAAA GTTTGCCCGG 
TCAGATGAAT TAGTCCGCCA TCACAACATG CATCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WT1
Entrez Gene ID	7490
Full Name	Wilms tumor 1
Synonyms	AWT1,GUD,NPHS4,WAGR,WIT-2,WT33
General protein information	Preferred Names Wilms tumor 1 Names Wilms tumor protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p13
Summary	This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015].
Disorder MIM:	607102
Disorder Html:	Wilms tumor, type 1, 194070 (3); Denys-Drash syndrome, 194080 (3); Nephrotic syndrome, type 4, 256370 (3); Frasier syndrome, 136680 (3); Meacham syndrome, 608978 (3); Mesothelioma, somatic, 156240 (3)

Bos taurus (cattle)	WT1	XP_003587050.2
Homo sapiens (human)	WT1	NP_077742.2
Mus musculus (house mouse)	Wt1	NP_659032.3
Gallus gallus (chicken)	WT1	NP_990547.1
Danio rerio (zebrafish)	wt1a	NP_571121.1
	WT1	XP_001084704.2
Rattus norvegicus (Norway rat)	Wt1	NP_113722.2
Xenopus tropicalis (tropical clawed frog)	wt1	NP_001135625.1
Pan troglodytes (chimpanzee)	WT1	XP_001138640.3
Canis lupus familiaris (dog)	WT1	XP_005631206.1

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WT1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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