BEST1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BEST1
Entrez Gene ID	7439
Full Name	bestrophin 1
Synonyms	ARB,BEST,BMD,RP50,TU15B,VMD2
General protein information	Preferred Names bestrophin 1 Names bestrophin-1 Best disease Best1V1Delta2 vitelliform macular dystrophy protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.3
Summary	This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008].
Disorder MIM:	607854
Disorder Html:	Macular dystrophy, vitelliform, 2, 153700 (3); Bestrophinopathy, autosomal recessive, 611809 (3); Vitreoretinochoroidopathy, 193220 (3); Microcornea, rod-cone dystrophy, cataract, and posterior staphyloma, 193220 (3); Retinitis pigmentosa-50, 613194 (3); Retinitis pigmentosa, concentric, 613194 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001139443.1	NP_001132915.1	bestrophin-1 isoform 2
XM_011545229.3	XP_011543531.1	bestrophin-1 isoform X1
XM_011545233.3	XP_011543535.1	bestrophin-1 isoform X6
XM_005274219.4	XP_005274276.1	bestrophin-1 isoform X9
XM_017018228.1	XP_016873717.1	bestrophin-1 isoform X3
XM_017018229.1	XP_016873718.1	bestrophin-1 isoform X5
XM_005274219.3	XP_005274276.1	bestrophin-1 isoform X9
XM_005274216.3	XP_005274273.1	bestrophin-1 isoform X7
NM_001363591.1	NP_001350520.1	bestrophin-1 isoform 5
XM_005274210.4	XP_005274267.1	bestrophin-1 isoform X1
XM_005274221.4	XP_005274278.1	bestrophin-1 isoform X10
NM_001139443.2	NP_001132915.1	bestrophin-1 isoform 2
NM_001363593.2	NP_001350522.1	bestrophin-1 isoform 7
NM_001300787.1	NP_001287716.1	bestrophin-1 isoform 4
XM_005274210.3	XP_005274267.1	bestrophin-1 isoform X1
XM_011545229.2	XP_011543531.1	bestrophin-1 isoform X1
NM_001363593.1	NP_001350522.1	bestrophin-1 isoform 7
XM_005274215.4	XP_005274272.1	bestrophin-1 isoform X4
XM_017018230.2	XP_016873719.1	bestrophin-1 isoform X8
NM_001363592.1	NP_001350521.1	bestrophin-1 isoform 6
NM_004183.4	NP_004174.1	bestrophin-1 isoform 1
NM_001363591.2	NP_001350520.1	bestrophin-1 isoform 5
XM_011545230.2	XP_011543532.1	bestrophin-1 isoform X2
XM_011545233.2	XP_011543535.1	bestrophin-1 isoform X6
XM_017018230.1	XP_016873719.1	bestrophin-1 isoform X8
NM_001300786.1	NP_001287715.1	bestrophin-1 isoform 3
NM_004183.3	NP_004174.1	bestrophin-1 isoform 1
XM_005274216.4	XP_005274273.1	bestrophin-1 isoform X7
XM_005274215.3	XP_005274272.1	bestrophin-1 isoform X4
XM_005274221.3	XP_005274278.1	bestrophin-1 isoform X10
XM_011545230.3	XP_011543532.1	bestrophin-1 isoform X2
NM_001300787.2	NP_001287716.1	bestrophin-1 isoform 4
NM_001300786.2	NP_001287715.1	bestrophin-1 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	BEST1	NP_004174.1
Bos taurus (cattle)	BEST1	NP_001073714.1
Mus musculus (house mouse)	Best1	NP_036043.2
Danio rerio (zebrafish)	LOC560608	XP_689098.3
Pan troglodytes (chimpanzee)	BEST1	XP_001151529.1
	LOC718993	XP_001116591.1
Xenopus tropicalis (tropical clawed frog)	best1	NP_001155223.1
Canis lupus familiaris (dog)	BEST1	NP_001091014.1
Rattus norvegicus (Norway rat)	Best1	NP_001011940.1
Gallus gallus (chicken)	BEST1	XP_004941545.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variations in Bestrophin?1 and associated clinical findings in two Chinese patients with juvenile?onset and adult?onset best vitelliform macular dystrophy.
Lin Y, Li T, Ma C, Gao H, Chen C, Zhu Y, Liu B, Lian Y, Huang Y, Li H, Wu Q, Liang X, Jin C, Huang X, Ye J, Lu L
Molecular medicine reports17(1)225-233(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We present a consanguineous family of five affected individuals with autosomal recessive bestrophinopathy and four confirmed carriers. Their pedigree was consistent with dominant inheritance and incomplete penetrance.
Title: A unique case series of autosomal recessive bestrophinopathy exhibiting multigenerational inheritance.

For patients with Best vitelliform macular dystrophy, single heterozygous BEST1 mutations were identified in 13 patients and compound heterozygous mutations were found in 3 patients. For patients with autosomal recessive bestrophinopathy, biallelic mutations were found in 13 probands and single mutant alleles in six patients. Overall, 36 disease-causing variants (20 novel mutations) of the BEST1 gene were identified.
Title: Screening of BEST1 Gene in a Chinese Cohort With Best Vitelliform Macular Dystrophy or Autosomal Recessive Bestrophinopathy.

Of the 225 genetic tests performed, 150 were for recessive IRD, and 75 were for dominant IRD. A positive molecular diagnosis was made in 70 (59%) of probands with recessive IRD and 19 (26%) probands with dominant IRD. Thirty-two novel variants were identified; among these, 17 sequence changes in four genes were predicted to be possibly or probably damaging including: ABCA4 (14), BEST1 (2), PRPH2 (1), and TIMP3
Title: A Report on Molecular Diagnostic Testing for Inherited Retinal Dystrophies by Targeted Genetic Analyses.

The secondary structure of Best1 and the effect of calcium have been described.
Title: Effects of Ca

Two previously unreported disease-associated variants in the BEST1 gene (p.Gly15Arg and p.Arg105Gly) were found in Slovenian patients with Best disease.
Title: Clinical and genetic heterogeneity in Slovenian patients with BEST disease.

The following BEST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19841	NM_004183.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu19927	NM_001139443.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu45486	NM_001300787.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu45487	XM_005274215.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu61311	NM_001363591.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61312	NM_001363592.1 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu61314	NM_001300786.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu45482	XM_005274210.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu45482	XM_011545229.3 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu45488	XM_005274216.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu45490	XM_017018230.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu45491	XM_005274219.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu45492	XM_005274221.4 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu61310	XM_011545230.3 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61311	XM_017018228.1 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61312	XM_017018229.1 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu61313	XM_011545233.3 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu107873	NM_001363593.2 Latest version!	Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu45491	XM_005274219.3	Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu19927	NM_001139443.1	Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu45482	XM_005274210.3	Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu45482	XM_011545229.2	Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu107873	NM_001363593.1	Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu61311	NM_001363591.1	Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu61313	XM_011545233.2	Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu45490	XM_017018230.1	Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu19841	NM_004183.3	Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu45488	XM_005274216.3	Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu45487	XM_005274215.3	Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu45492	XM_005274221.3	Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu61310	XM_011545230.2	Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu45486	NM_001300787.1	Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu61314	NM_001300786.1	Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEST1 ( NM_004183.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004174.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.

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CloneID	OHu19841
Clone ID Related Accession (Same CDS sequence)	NM_004183.4 , NM_004183.3
Accession Version	NM_004183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2 and AF057169.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004183.3. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (1) is longer at the N-terminus and has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF057169.1, SRR1803614.72816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378043.9/ ENSP00000367282.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004174.1
CDS	114..1871
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_004183.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004183.4

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_004183.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004174.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19841
Clone ID Related Accession (Same CDS sequence)	NM_004183.4 , NM_004183.3
Accession Version	NM_004183.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2 and AF057169.1. This sequence is a reference standard in the RefSeqGene project. On Nov 14, 2008 this sequence version replaced NM_004183.2. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (1) is longer at the N-terminus and has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.55361.1, SRR1803614.72816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004174.1
CDS	581..2338
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_004183.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004183.3

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001139443.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001132915.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.

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CloneID	OHu19927
Clone ID Related Accession (Same CDS sequence)	NM_001139443.1 , NM_001139443.2
Accession Version	NM_001139443.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2, BC041664.1 and AF057169.1. Transcript Variant: This variant (2) encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041664.1, SRR1660809.76219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001132915.1
CDS	573..2387
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001139443.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001139443.1

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001139443.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001132915.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19927
Clone ID Related Accession (Same CDS sequence)	NM_001139443.1 , NM_001139443.2
Accession Version	NM_001139443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2, BC041664.1, AF057169.1 and AP003733.5. On May 31, 2019 this sequence version replaced NM_001139443.1. Transcript Variant: This variant (2) encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.9336.1, SRR1660809.76219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001132915.1
CDS	106..1920
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001139443.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001139443.2

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001300787.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287716.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu45486
Clone ID Related Accession (Same CDS sequence)	NM_001300787.1 , NM_001300787.2
Accession Version	NM_001300787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2, AK295998.1 and AP003733.5. On Jul 12, 2014 this sequence version replaced XM_005274213.1. Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.67883.1, SRR1660807.66523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287716.1
CDS	573..2150
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_001300787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300787.1

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001300787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287716.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.

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CloneID	OHu45486
Clone ID Related Accession (Same CDS sequence)	NM_001300787.1 , NM_001300787.2
Accession Version	NM_001300787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295998.1, AP003733.5 and BC015220.2. On Jun 1, 2019 this sequence version replaced NM_001300787.1. Transcript Variant: This variant (4) differs in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.67883.1, AK295998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287716.1
CDS	106..1683
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_001300787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300787.2

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
GATGAAGCAC ATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274215.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274272.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu45487
Clone ID Related Accession (Same CDS sequence)	XM_005274215.4 , XM_005274215.3
Accession Version	XM_005274215.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274215.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGGATGAAGC ACATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274272.1
CDS	664..2103
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_005274215.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274215.4

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGGATGAAGC ACATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274215.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274272.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu45487
Clone ID Related Accession (Same CDS sequence)	XM_005274215.4 , XM_005274215.3
Accession Version	XM_005274215.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274215.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGGATGAAGC ACATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274272.1
CDS	664..2103
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_005274215.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274215.3

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGGATGAAGC ACATTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001363591.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350520.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu61311
Clone ID Related Accession (Same CDS sequence)	XM_017018228.1 , NM_001363591.1 , NM_001363591.2
Accession Version	NM_001363591.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003733.5. On May 24, 2018 this sequence version replaced XM_017018228.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.182113.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350520.1
CDS	664..2340
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001363591.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363591.1

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001363591.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350520.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu61311
Clone ID Related Accession (Same CDS sequence)	XM_017018228.1 , NM_001363591.1 , NM_001363591.2
Accession Version	NM_001363591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003733.5. On May 31, 2019 this sequence version replaced NM_001363591.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.182113.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350520.1
CDS	225..1901
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001363591.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363591.2

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001363592.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350521.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu61312
Clone ID Related Accession (Same CDS sequence)	XM_017018229.1 , NM_001363592.1
Accession Version	NM_001363592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003733.5 and AK289681.1. On May 24, 2018 this sequence version replaced XM_017018229.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.184542.1, SRR1660809.219985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350521.1
CDS	644..1951
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	NM_001363592.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363592.1

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001300786.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287715.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.

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CloneID	OHu61314
Clone ID Related Accession (Same CDS sequence)	NM_001300786.1 , NM_001300786.2
Accession Version	NM_001300786.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2 and AP003733.5. Transcript Variant: This variant (3) lacks an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (3) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015220.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG TCCCTGTTGG CTGTGGATGA GATGCACCAG 
GACCTGCCTC GGATGGAGCC GGACATGTAC TGGAATAAGC CCGAGCCACA GCCCCCCTAC 
ACAGCTGCTT CCGCCCAGTT CCGTCGAGCC TCCTTTATGG GCTCCACCTT CAACATCAGC 
CTGAACAAAG AGGAGATGGA GTTCCAGCCC AATCAGGAGG ACGAGGAGGA TGCTCACGCT 
GGCATCATTG GCCGCTTCCT AGGCCTGCAG TCCCATGATC ACCATCCTCC CAGGGCAAAC 
TCAAGGACCA AACTACTGTG GCCCAAGAGG GAATCCCTTC TCCACGAGGG CCTGCCCAAA 
AACCACAAGG CAGCCAAACA GAACGTTAGG GGCCAGGAAG ACAACAAGGC CTGGAAGCTT 
AAGGCTGTGG ACGCCTTCAA GTCTGCCCCA CTGTATCAGA GGCCAGGCTA CTACAGTGCC 
CCACAGACGC CCCTCAGCCC CACTCCCATG TTCTTCCCCC TAGAACCATC AGCGCCGTCA 
AAGCTTCACA GTGTCACAGG CATAGACACC AAAGACAAAA GCTTAAAGAC TGTGAGTTCT 
GGGGCCAAGA AAAGTTTTGA ATTGCTCTCA GAGAGCGATG GGGCCTTGAT GGAGCACCCA 
GAAGTATCTC AAGTGAGGAG GAAAACTGTG GAGTTTAACC TGACGGATAT GCCAGAGATC 
CCCGAAAATC ACCTCAAAGA ACCTTTGGAA CAATCACCAA CCAACATACA CACTACACTC 
AAAGATCACA TGGATCCTTA TTGGGCCTTG GAAAACAGGG ATGAAGCACA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287715.1
CDS	573..2069
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_001300786.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300786.1

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG TCCCTGTTGG CTGTGGATGA GATGCACCAG 
GACCTGCCTC GGATGGAGCC GGACATGTAC TGGAATAAGC CCGAGCCACA GCCCCCCTAC 
ACAGCTGCTT CCGCCCAGTT CCGTCGAGCC TCCTTTATGG GCTCCACCTT CAACATCAGC 
CTGAACAAAG AGGAGATGGA GTTCCAGCCC AATCAGGAGG ACGAGGAGGA TGCTCACGCT 
GGCATCATTG GCCGCTTCCT AGGCCTGCAG TCCCATGATC ACCATCCTCC CAGGGCAAAC 
TCAAGGACCA AACTACTGTG GCCCAAGAGG GAATCCCTTC TCCACGAGGG CCTGCCCAAA 
AACCACAAGG CAGCCAAACA GAACGTTAGG GGCCAGGAAG ACAACAAGGC CTGGAAGCTT 
AAGGCTGTGG ACGCCTTCAA GTCTGCCCCA CTGTATCAGA GGCCAGGCTA CTACAGTGCC 
CCACAGACGC CCCTCAGCCC CACTCCCATG TTCTTCCCCC TAGAACCATC AGCGCCGTCA 
AAGCTTCACA GTGTCACAGG CATAGACACC AAAGACAAAA GCTTAAAGAC TGTGAGTTCT 
GGGGCCAAGA AAAGTTTTGA ATTGCTCTCA GAGAGCGATG GGGCCTTGAT GGAGCACCCA 
GAAGTATCTC AAGTGAGGAG GAAAACTGTG GAGTTTAACC TGACGGATAT GCCAGAGATC 
CCCGAAAATC ACCTCAAAGA ACCTTTGGAA CAATCACCAA CCAACATACA CACTACACTC 
AAAGATCACA TGGATCCTTA TTGGGCCTTG GAAAACAGGG ATGAAGCACA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001300786.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287715.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.

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CloneID	OHu61314
Clone ID Related Accession (Same CDS sequence)	NM_001300786.1 , NM_001300786.2
Accession Version	NM_001300786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015220.2 and AP003733.5. On May 31, 2019 this sequence version replaced NM_001300786.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 2. The resulting isoform (3) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC015220.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG TCCCTGTTGG CTGTGGATGA GATGCACCAG 
GACCTGCCTC GGATGGAGCC GGACATGTAC TGGAATAAGC CCGAGCCACA GCCCCCCTAC 
ACAGCTGCTT CCGCCCAGTT CCGTCGAGCC TCCTTTATGG GCTCCACCTT CAACATCAGC 
CTGAACAAAG AGGAGATGGA GTTCCAGCCC AATCAGGAGG ACGAGGAGGA TGCTCACGCT 
GGCATCATTG GCCGCTTCCT AGGCCTGCAG TCCCATGATC ACCATCCTCC CAGGGCAAAC 
TCAAGGACCA AACTACTGTG GCCCAAGAGG GAATCCCTTC TCCACGAGGG CCTGCCCAAA 
AACCACAAGG CAGCCAAACA GAACGTTAGG GGCCAGGAAG ACAACAAGGC CTGGAAGCTT 
AAGGCTGTGG ACGCCTTCAA GTCTGCCCCA CTGTATCAGA GGCCAGGCTA CTACAGTGCC 
CCACAGACGC CCCTCAGCCC CACTCCCATG TTCTTCCCCC TAGAACCATC AGCGCCGTCA 
AAGCTTCACA GTGTCACAGG CATAGACACC AAAGACAAAA GCTTAAAGAC TGTGAGTTCT 
GGGGCCAAGA AAAGTTTTGA ATTGCTCTCA GAGAGCGATG GGGCCTTGAT GGAGCACCCA 
GAAGTATCTC AAGTGAGGAG GAAAACTGTG GAGTTTAACC TGACGGATAT GCCAGAGATC 
CCCGAAAATC ACCTCAAAGA ACCTTTGGAA CAATCACCAA CCAACATACA CACTACACTC 
AAAGATCACA TGGATCCTTA TTGGGCCTTG GAAAACAGGG ATGAAGCACA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287715.1
CDS	106..1602
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_001300786.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300786.2

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG TCCCTGTTGG CTGTGGATGA GATGCACCAG 
GACCTGCCTC GGATGGAGCC GGACATGTAC TGGAATAAGC CCGAGCCACA GCCCCCCTAC 
ACAGCTGCTT CCGCCCAGTT CCGTCGAGCC TCCTTTATGG GCTCCACCTT CAACATCAGC 
CTGAACAAAG AGGAGATGGA GTTCCAGCCC AATCAGGAGG ACGAGGAGGA TGCTCACGCT 
GGCATCATTG GCCGCTTCCT AGGCCTGCAG TCCCATGATC ACCATCCTCC CAGGGCAAAC 
TCAAGGACCA AACTACTGTG GCCCAAGAGG GAATCCCTTC TCCACGAGGG CCTGCCCAAA 
AACCACAAGG CAGCCAAACA GAACGTTAGG GGCCAGGAAG ACAACAAGGC CTGGAAGCTT 
AAGGCTGTGG ACGCCTTCAA GTCTGCCCCA CTGTATCAGA GGCCAGGCTA CTACAGTGCC 
CCACAGACGC CCCTCAGCCC CACTCCCATG TTCTTCCCCC TAGAACCATC AGCGCCGTCA 
AAGCTTCACA GTGTCACAGG CATAGACACC AAAGACAAAA GCTTAAAGAC TGTGAGTTCT 
GGGGCCAAGA AAAGTTTTGA ATTGCTCTCA GAGAGCGATG GGGCCTTGAT GGAGCACCCA 
GAAGTATCTC AAGTGAGGAG GAAAACTGTG GAGTTTAACC TGACGGATAT GCCAGAGATC 
CCCGAAAATC ACCTCAAAGA ACCTTTGGAA CAATCACCAA CCAACATACA CACTACACTC 
AAAGATCACA TGGATCCTTA TTGGGCCTTG GAAAACAGGG ATGAAGCACA TTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545229.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543531.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.

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CloneID	OHu45482
Clone ID Related Accession (Same CDS sequence)	XM_011545229.3 , XM_005274210.4 , XM_005274210.3 , XM_011545229.2
Accession Version	XM_011545229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543531.1
CDS	131..2125
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545229.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545229.3

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274210.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274267.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu45482
Clone ID Related Accession (Same CDS sequence)	XM_011545229.3 , XM_005274210.4 , XM_005274210.3 , XM_011545229.2
Accession Version	XM_005274210.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274210.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274267.1
CDS	691..2685
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_005274210.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274210.4

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274210.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274267.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu45482
Clone ID Related Accession (Same CDS sequence)	XM_011545229.3 , XM_005274210.4 , XM_005274210.3 , XM_011545229.2
Accession Version	XM_005274210.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274267.1
CDS	691..2685
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_005274210.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274210.3

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545229.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543531.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.

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CloneID	OHu45482
Clone ID Related Accession (Same CDS sequence)	XM_011545229.3 , XM_005274210.4 , XM_005274210.3 , XM_011545229.2
Accession Version	XM_011545229.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545229.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543531.1
CDS	131..2125
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545229.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545229.2

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GCAGAGCAGC TCATCAACCC CTTTGGAGAG 
GATGATGATG ATTTTGAGAC CAACTGGATT GTCGACAGGA ATTTGCAGGT GTCCCTGTTG 
GCTGTGGATG AGATGCACCA GGACCTGCCT CGGATGGAGC CGGACATGTA CTGGAATAAG 
CCCGAGCCAC AGCCCCCCTA CACAGCTGCT TCCGCCCAGT TCCGTCGAGC CTCCTTTATG 
GGCTCCACCT TCAACATCAG CCTGAACAAA GAGGAGATGG AGTTCCAGCC CAATCAGGAG 
GACGAGGAGG ATGCTCACGC TGGCATCATT GGCCGCTTCC TAGGCCTGCA GTCCCATGAT 
CACCATCCTC CCAGGGCAAA CTCAAGGACC AAACTACTGT GGCCCAAGAG GGAATCCCTT 
CTCCACGAGG GCCTGCCCAA AAACCACAAG GCAGCCAAAC AGAACGTTAG GGGCCAGGAA 
GACAACAAGG CCTGGAAGCT TAAGGCTGTG GACGCCTTCA AGTCTGCCCC ACTGTATCAG 
AGGCCAGGCT ACTACAGTGC CCCACAGACG CCCCTCAGCC CCACTCCCAT GTTCTTCCCC 
CTAGAACCAT CAGCGCCGTC AAAGCTTCAC AGTGTCACAG GCATAGACAC CAAAGACAAA 
AGCTTAAAGA CTGTGAGTTC TGGGGCCAAG AAAAGTTTTG AATTGCTCTC AGAGAGCGAT 
GGGGCCTTGA TGGAGCACCC AGAAGTATCT CAAGTGAGGA GGAAAACTGT GGAGTTTAAC 
CTGACGGATA TGCCAGAGAT CCCCGAAAAT CACCTCAAAG AACCTTTGGA ACAATCACCA 
ACCAACATAC ACACTACACT CAAAGATCAC ATGGATCCTT ATTGGGCCTT GGAAAACAGG 
TCTGTCCTCC ACCTGAACCA GGGGCACTGC ATTGCCCTGT GCCCCACCCC AGCTTCCCTT 
GCTCTGAGCC TACCCTTCCT CCACAATTTC CTAGGGTTCC ATCACTGCCA GAGCACACTG 
GACCTACGCC CAGCACTGGC TTGGGGTATA TACTTGGCCA CCTTCACAGG GATCCTAGGG 
AAGTGTTCGG GACCTTTTCT CACTTCACCC TGGTATCACC CGGAAGACTT CTTGGGACCA 
GGTGAAGGAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274216.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274273.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu45488
Clone ID Related Accession (Same CDS sequence)	XM_005274216.3 , XM_005274216.4
Accession Version	XM_005274216.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274273.1
CDS	683..1810
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	XM_005274216.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274216.3

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274216.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274273.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu45488
Clone ID Related Accession (Same CDS sequence)	XM_005274216.3 , XM_005274216.4
Accession Version	XM_005274216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274216.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274273.1
CDS	679..1806
Translation	MFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	XM_005274216.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274216.4

ORF Insert Sequence:

ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_017018230.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873719.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu45490
Clone ID Related Accession (Same CDS sequence)	XM_017018230.2 , XM_017018230.1
Accession Version	XM_017018230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGGCCTCTC CAGGGCCCTG CTGGGCTGGA GGCATGGCCA GAGGGGTCAT 
GGCCAGCAGC TGCCTGAGAC GAGGATGCAG TGTCAGGAAA GGAAGGTCTC ACGGGTAGAA 
AGCAGCCAGG CGTGGTGGCG CACACCTGTA ATCCCAGCTA CTCGGGAGGC TGAGGCAGGA 
GAATCGCTTG AACCCGGGAG GCGGAGGTTG TGGTGGCAGA GCAGCTCATC AACCCCTTTG 
GAGAGGATGA TGATGATTTT GAGACCAACT GGATTGTCGA CAGGAATTTG CAGGTGTCCC 
TGTTGGCTGT GGATGAGATG CACCAGGACC TGCCTCGGAT GGAGCCGGAC ATGTACTGGA 
ATAAGCCCGA GCCACAGCCC CCCTACACAG CTGCTTCCGC CCAGTTCCGT CGAGCCTCCT 
TTATGGGCTC CACCTTCAAC ATCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873719.1
CDS	664..1653
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	XM_017018230.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018230.2

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGGCCTCTC CAGGGCCCTG CTGGGCTGGA GGCATGGCCA GAGGGGTCAT 
GGCCAGCAGC TGCCTGAGAC GAGGATGCAG TGTCAGGAAA GGAAGGTCTC ACGGGTAGAA 
AGCAGCCAGG CGTGGTGGCG CACACCTGTA ATCCCAGCTA CTCGGGAGGC TGAGGCAGGA 
GAATCGCTTG AACCCGGGAG GCGGAGGTTG TGGTGGCAGA GCAGCTCATC AACCCCTTTG 
GAGAGGATGA TGATGATTTT GAGACCAACT GGATTGTCGA CAGGAATTTG CAGGTGTCCC 
TGTTGGCTGT GGATGAGATG CACCAGGACC TGCCTCGGAT GGAGCCGGAC ATGTACTGGA 
ATAAGCCCGA GCCACAGCCC CCCTACACAG CTGCTTCCGC CCAGTTCCGT CGAGCCTCCT 
TTATGGGCTC CACCTTCAAC ATCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_017018230.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873719.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu45490
Clone ID Related Accession (Same CDS sequence)	XM_017018230.2 , XM_017018230.1
Accession Version	XM_017018230.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGGCCTCTC CAGGGCCCTG CTGGGCTGGA GGCATGGCCA GAGGGGTCAT 
GGCCAGCAGC TGCCTGAGAC GAGGATGCAG TGTCAGGAAA GGAAGGTCTC ACGGGTAGAA 
AGCAGCCAGG CGTGGTGGCG CACACCTGTA ATCCCAGCTA CTCGGGAGGC TGAGGCAGGA 
GAATCGCTTG AACCCGGGAG GCGGAGGTTG TGGTGGCAGA GCAGCTCATC AACCCCTTTG 
GAGAGGATGA TGATGATTTT GAGACCAACT GGATTGTCGA CAGGAATTTG CAGGTGTCCC 
TGTTGGCTGT GGATGAGATG CACCAGGACC TGCCTCGGAT GGAGCCGGAC ATGTACTGGA 
ATAAGCCCGA GCCACAGCCC CCCTACACAG CTGCTTCCGC CCAGTTCCGT CGAGCCTCCT 
TTATGGGCTC CACCTTCAAC ATCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873719.1
CDS	664..1653
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	XM_017018230.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018230.1

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGGCCTCTC CAGGGCCCTG CTGGGCTGGA GGCATGGCCA GAGGGGTCAT 
GGCCAGCAGC TGCCTGAGAC GAGGATGCAG TGTCAGGAAA GGAAGGTCTC ACGGGTAGAA 
AGCAGCCAGG CGTGGTGGCG CACACCTGTA ATCCCAGCTA CTCGGGAGGC TGAGGCAGGA 
GAATCGCTTG AACCCGGGAG GCGGAGGTTG TGGTGGCAGA GCAGCTCATC AACCCCTTTG 
GAGAGGATGA TGATGATTTT GAGACCAACT GGATTGTCGA CAGGAATTTG CAGGTGTCCC 
TGTTGGCTGT GGATGAGATG CACCAGGACC TGCCTCGGAT GGAGCCGGAC ATGTACTGGA 
ATAAGCCCGA GCCACAGCCC CCCTACACAG CTGCTTCCGC CCAGTTCCGT CGAGCCTCCT 
TTATGGGCTC CACCTTCAAC ATCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274219.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274276.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu45491
Clone ID Related Accession (Same CDS sequence)	XM_005274219.4 , XM_005274219.3
Accession Version	XM_005274219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274219.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA 
TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274276.1
CDS	691..1656
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKPEQRGDGVPAQSGGRGGCSRWHHWPLPRPAVP

Target ORF information:

RefSeq Version	XM_005274219.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274219.4

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA 
TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274219.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274276.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu45491
Clone ID Related Accession (Same CDS sequence)	XM_005274219.4 , XM_005274219.3
Accession Version	XM_005274219.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA 
TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274276.1
CDS	691..1656
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKPEQRGDGVPAQSGGRGGCSRWHHWPLPRPAVP

Target ORF information:

RefSeq Version	XM_005274219.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274219.3

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA 
TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274221.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274278.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu45492
Clone ID Related Accession (Same CDS sequence)	XM_005274221.4 , XM_005274221.3
Accession Version	XM_005274221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005274221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGCCTGAA 
CAAAGAGGAG ATGGAGTTCC AGCCCAATCA GGAGGACGAG GAGGATGCTC ACGCTGGCAT 
CATTGGCCGC TTCCTAGGCC TGCAGTCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274278.1
CDS	691..1503
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQPEQRGDGVPAQSGGRGGCSRWHHWPLPRPAVP

Target ORF information:

RefSeq Version	XM_005274221.4
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274221.4

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGCCTGAA 
CAAAGAGGAG ATGGAGTTCC AGCCCAATCA GGAGGACGAG GAGGATGCTC ACGCTGGCAT 
CATTGGCCGC TTCCTAGGCC TGCAGTCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_005274221.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274278.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.

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CloneID	OHu45492
Clone ID Related Accession (Same CDS sequence)	XM_005274221.4 , XM_005274221.3
Accession Version	XM_005274221.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGCCTGAA 
CAAAGAGGAG ATGGAGTTCC AGCCCAATCA GGAGGACGAG GAGGATGCTC ACGCTGGCAT 
CATTGGCCGC TTCCTAGGCC TGCAGTCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274278.1
CDS	691..1503
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQPEQRGDGVPAQSGGRGGCSRWHHWPLPRPAVP

Target ORF information:

RefSeq Version	XM_005274221.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274221.3

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGCCTGAA 
CAAAGAGGAG ATGGAGTTCC AGCCCAATCA GGAGGACGAG GAGGATGCTC ACGCTGGCAT 
CATTGGCCGC TTCCTAGGCC TGCAGTCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545230.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543532.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.

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CloneID	OHu61310
Clone ID Related Accession (Same CDS sequence)	XM_011545230.2 , XM_011545230.3
Accession Version	XM_011545230.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCC CATCCTTTGG ACCTCCCTTC AACCCCAAGA GAGACCACTA CCCTAGCAGG 
CTGGCCCTCA CGGAAGAACA ACAGCTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACGCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAG CCTGGTGTCG 
GGCTTCGTCG AAGGCAAGGA CGAGCAAGGC CGGCTGCTGC GGCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCA CCGCAGTCTA CAAGCGCTTC 
CCCAGCGCCC AGCACCTGGT GCAAGCAGGC TTTATGACTC CGGCAGAACA CAAGCAGTTG 
GAGAAACTGA GCCTACCACA CAACATGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGCTTGG AGGTCGAATC CGGGACCCTA TCCTGCTCCA GAGCCTGCTG 
AACGAGATGA ACACCTTGCG TACTCAGTGT GGACACCTGT ATGCCTACGA CTGGATTAGT 
ATCCCACTGG TGTATACACA GGTGGTGACT GTGGCGGTGT ACAGCTTCTT CCTGACTTGT 
CTAGTTGGGC GGCAGTTTCT GAACCCAGCC AAGGCCTACC CTGGCCATGA GCTGGACCTC 
GTTGTGCCCG TCTTCACGTT CCTGCAGTTC TTCTTCTATG TTGGCTGGCT GAAGGTGGCA 
GAGCAGCTCA TCAACCCCTT TGGAGAGGAT GATGATGATT TTGAGACCAA CTGGATTGTC 
GACAGGAATT TGCAGGTGTC CCTGTTGGCT GTGGATGAGA TGCACCAGGA CCTGCCTCGG 
ATGGAGCCGG ACATGTACTG GAATAAGCCC GAGCCACAGC CCCCCTACAC AGCTGCTTCC 
GCCCAGTTCC GTCGAGCCTC CTTTATGGGC TCCACCTTCA ACATCAGCCT GAACAAAGAG 
GAGATGGAGT TCCAGCCCAA TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC 
CGCTTCCTAG GCCTGCAGTC CCATGATCAC CATCCTCCCA GGGCAAACTC AAGGACCAAA 
CTACTGTGGC CCAAGAGGGA ATCCCTTCTC CACGAGGGCC TGCCCAAAAA CCACAAGGCA 
GCCAAACAGA ACGTTAGGGG CCAGGAAGAC AACAAGGCCT GGAAGCTTAA GGCTGTGGAC 
GCCTTCAAGT CTGCCCCACT GTATCAGAGG CCAGGCTACT ACAGTGCCCC ACAGACGCCC 
CTCAGCCCCA CTCCCATGTT CTTCCCCCTA GAACCATCAG CGCCGTCAAA GCTTCACAGT 
GTCACAGGCA TAGACACCAA AGACAAAAGC TTAAAGACTG TGAGTTCTGG GGCCAAGAAA 
AGTTTTGAAT TGCTCTCAGA GAGCGATGGG GCCTTGATGG AGCACCCAGA AGTATCTCAA 
GTGAGGAGGA AAACTGTGGA GTTTAACCTG ACGGATATGC CAGAGATCCC CGAAAATCAC 
CTCAAAGAAC CTTTGGAACA ATCACCAACC AACATACACA CTACACTCAA AGATCACATG 
GATCCTTATT GGGCCTTGGA AAACAGGTCT GTCCTCCACC TGAACCAGGG GCACTGCATT 
GCCCTGTGCC CCACCCCAGC TTCCCTTGCT CTGAGCCTAC CCTTCCTCCA CAATTTCCTA 
GGGTTCCATC ACTGCCAGAG CACACTGGAC CTACGCCCAG CACTGGCTTG GGGTATATAC 
TTGGCCACCT TCACAGGGAT CCTAGGGAAG TGTTCGGGAC CTTTTCTCAC TTCACCCTGG 
TATCACCCGG AAGACTTCTT GGGACCAGGT GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543532.1
CDS	27..1928
Translation	MQAPSFGPPFNPKRDHYPSRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545230.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545230.2

ORF Insert Sequence:

ATGCAGGCCC CATCCTTTGG ACCTCCCTTC AACCCCAAGA GAGACCACTA CCCTAGCAGG 
CTGGCCCTCA CGGAAGAACA ACAGCTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACGCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAG CCTGGTGTCG 
GGCTTCGTCG AAGGCAAGGA CGAGCAAGGC CGGCTGCTGC GGCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCA CCGCAGTCTA CAAGCGCTTC 
CCCAGCGCCC AGCACCTGGT GCAAGCAGGC TTTATGACTC CGGCAGAACA CAAGCAGTTG 
GAGAAACTGA GCCTACCACA CAACATGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGCTTGG AGGTCGAATC CGGGACCCTA TCCTGCTCCA GAGCCTGCTG 
AACGAGATGA ACACCTTGCG TACTCAGTGT GGACACCTGT ATGCCTACGA CTGGATTAGT 
ATCCCACTGG TGTATACACA GGTGGTGACT GTGGCGGTGT ACAGCTTCTT CCTGACTTGT 
CTAGTTGGGC GGCAGTTTCT GAACCCAGCC AAGGCCTACC CTGGCCATGA GCTGGACCTC 
GTTGTGCCCG TCTTCACGTT CCTGCAGTTC TTCTTCTATG TTGGCTGGCT GAAGGTGGCA 
GAGCAGCTCA TCAACCCCTT TGGAGAGGAT GATGATGATT TTGAGACCAA CTGGATTGTC 
GACAGGAATT TGCAGGTGTC CCTGTTGGCT GTGGATGAGA TGCACCAGGA CCTGCCTCGG 
ATGGAGCCGG ACATGTACTG GAATAAGCCC GAGCCACAGC CCCCCTACAC AGCTGCTTCC 
GCCCAGTTCC GTCGAGCCTC CTTTATGGGC TCCACCTTCA ACATCAGCCT GAACAAAGAG 
GAGATGGAGT TCCAGCCCAA TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC 
CGCTTCCTAG GCCTGCAGTC CCATGATCAC CATCCTCCCA GGGCAAACTC AAGGACCAAA 
CTACTGTGGC CCAAGAGGGA ATCCCTTCTC CACGAGGGCC TGCCCAAAAA CCACAAGGCA 
GCCAAACAGA ACGTTAGGGG CCAGGAAGAC AACAAGGCCT GGAAGCTTAA GGCTGTGGAC 
GCCTTCAAGT CTGCCCCACT GTATCAGAGG CCAGGCTACT ACAGTGCCCC ACAGACGCCC 
CTCAGCCCCA CTCCCATGTT CTTCCCCCTA GAACCATCAG CGCCGTCAAA GCTTCACAGT 
GTCACAGGCA TAGACACCAA AGACAAAAGC TTAAAGACTG TGAGTTCTGG GGCCAAGAAA 
AGTTTTGAAT TGCTCTCAGA GAGCGATGGG GCCTTGATGG AGCACCCAGA AGTATCTCAA 
GTGAGGAGGA AAACTGTGGA GTTTAACCTG ACGGATATGC CAGAGATCCC CGAAAATCAC 
CTCAAAGAAC CTTTGGAACA ATCACCAACC AACATACACA CTACACTCAA AGATCACATG 
GATCCTTATT GGGCCTTGGA AAACAGGTCT GTCCTCCACC TGAACCAGGG GCACTGCATT 
GCCCTGTGCC CCACCCCAGC TTCCCTTGCT CTGAGCCTAC CCTTCCTCCA CAATTTCCTA 
GGGTTCCATC ACTGCCAGAG CACACTGGAC CTACGCCCAG CACTGGCTTG GGGTATATAC 
TTGGCCACCT TCACAGGGAT CCTAGGGAAG TGTTCGGGAC CTTTTCTCAC TTCACCCTGG 
TATCACCCGG AAGACTTCTT GGGACCAGGT GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545230.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543532.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61310
Clone ID Related Accession (Same CDS sequence)	XM_011545230.2 , XM_011545230.3
Accession Version	XM_011545230.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCCC CATCCTTTGG ACCTCCCTTC AACCCCAAGA GAGACCACTA CCCTAGCAGG 
CTGGCCCTCA CGGAAGAACA ACAGCTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACGCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAG CCTGGTGTCG 
GGCTTCGTCG AAGGCAAGGA CGAGCAAGGC CGGCTGCTGC GGCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCA CCGCAGTCTA CAAGCGCTTC 
CCCAGCGCCC AGCACCTGGT GCAAGCAGGC TTTATGACTC CGGCAGAACA CAAGCAGTTG 
GAGAAACTGA GCCTACCACA CAACATGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGCTTGG AGGTCGAATC CGGGACCCTA TCCTGCTCCA GAGCCTGCTG 
AACGAGATGA ACACCTTGCG TACTCAGTGT GGACACCTGT ATGCCTACGA CTGGATTAGT 
ATCCCACTGG TGTATACACA GGTGGTGACT GTGGCGGTGT ACAGCTTCTT CCTGACTTGT 
CTAGTTGGGC GGCAGTTTCT GAACCCAGCC AAGGCCTACC CTGGCCATGA GCTGGACCTC 
GTTGTGCCCG TCTTCACGTT CCTGCAGTTC TTCTTCTATG TTGGCTGGCT GAAGGTGGCA 
GAGCAGCTCA TCAACCCCTT TGGAGAGGAT GATGATGATT TTGAGACCAA CTGGATTGTC 
GACAGGAATT TGCAGGTGTC CCTGTTGGCT GTGGATGAGA TGCACCAGGA CCTGCCTCGG 
ATGGAGCCGG ACATGTACTG GAATAAGCCC GAGCCACAGC CCCCCTACAC AGCTGCTTCC 
GCCCAGTTCC GTCGAGCCTC CTTTATGGGC TCCACCTTCA ACATCAGCCT GAACAAAGAG 
GAGATGGAGT TCCAGCCCAA TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC 
CGCTTCCTAG GCCTGCAGTC CCATGATCAC CATCCTCCCA GGGCAAACTC AAGGACCAAA 
CTACTGTGGC CCAAGAGGGA ATCCCTTCTC CACGAGGGCC TGCCCAAAAA CCACAAGGCA 
GCCAAACAGA ACGTTAGGGG CCAGGAAGAC AACAAGGCCT GGAAGCTTAA GGCTGTGGAC 
GCCTTCAAGT CTGCCCCACT GTATCAGAGG CCAGGCTACT ACAGTGCCCC ACAGACGCCC 
CTCAGCCCCA CTCCCATGTT CTTCCCCCTA GAACCATCAG CGCCGTCAAA GCTTCACAGT 
GTCACAGGCA TAGACACCAA AGACAAAAGC TTAAAGACTG TGAGTTCTGG GGCCAAGAAA 
AGTTTTGAAT TGCTCTCAGA GAGCGATGGG GCCTTGATGG AGCACCCAGA AGTATCTCAA 
GTGAGGAGGA AAACTGTGGA GTTTAACCTG ACGGATATGC CAGAGATCCC CGAAAATCAC 
CTCAAAGAAC CTTTGGAACA ATCACCAACC AACATACACA CTACACTCAA AGATCACATG 
GATCCTTATT GGGCCTTGGA AAACAGGTCT GTCCTCCACC TGAACCAGGG GCACTGCATT 
GCCCTGTGCC CCACCCCAGC TTCCCTTGCT CTGAGCCTAC CCTTCCTCCA CAATTTCCTA 
GGGTTCCATC ACTGCCAGAG CACACTGGAC CTACGCCCAG CACTGGCTTG GGGTATATAC 
TTGGCCACCT TCACAGGGAT CCTAGGGAAG TGTTCGGGAC CTTTTCTCAC TTCACCCTGG 
TATCACCCGG AAGACTTCTT GGGACCAGGT GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543532.1
CDS	28..1929
Translation	MQAPSFGPPFNPKRDHYPSRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545230.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545230.3

ORF Insert Sequence:

ATGCAGGCCC CATCCTTTGG ACCTCCCTTC AACCCCAAGA GAGACCACTA CCCTAGCAGG 
CTGGCCCTCA CGGAAGAACA ACAGCTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACGCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAG CCTGGTGTCG 
GGCTTCGTCG AAGGCAAGGA CGAGCAAGGC CGGCTGCTGC GGCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCA CCGCAGTCTA CAAGCGCTTC 
CCCAGCGCCC AGCACCTGGT GCAAGCAGGC TTTATGACTC CGGCAGAACA CAAGCAGTTG 
GAGAAACTGA GCCTACCACA CAACATGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGCTTGG AGGTCGAATC CGGGACCCTA TCCTGCTCCA GAGCCTGCTG 
AACGAGATGA ACACCTTGCG TACTCAGTGT GGACACCTGT ATGCCTACGA CTGGATTAGT 
ATCCCACTGG TGTATACACA GGTGGTGACT GTGGCGGTGT ACAGCTTCTT CCTGACTTGT 
CTAGTTGGGC GGCAGTTTCT GAACCCAGCC AAGGCCTACC CTGGCCATGA GCTGGACCTC 
GTTGTGCCCG TCTTCACGTT CCTGCAGTTC TTCTTCTATG TTGGCTGGCT GAAGGTGGCA 
GAGCAGCTCA TCAACCCCTT TGGAGAGGAT GATGATGATT TTGAGACCAA CTGGATTGTC 
GACAGGAATT TGCAGGTGTC CCTGTTGGCT GTGGATGAGA TGCACCAGGA CCTGCCTCGG 
ATGGAGCCGG ACATGTACTG GAATAAGCCC GAGCCACAGC CCCCCTACAC AGCTGCTTCC 
GCCCAGTTCC GTCGAGCCTC CTTTATGGGC TCCACCTTCA ACATCAGCCT GAACAAAGAG 
GAGATGGAGT TCCAGCCCAA TCAGGAGGAC GAGGAGGATG CTCACGCTGG CATCATTGGC 
CGCTTCCTAG GCCTGCAGTC CCATGATCAC CATCCTCCCA GGGCAAACTC AAGGACCAAA 
CTACTGTGGC CCAAGAGGGA ATCCCTTCTC CACGAGGGCC TGCCCAAAAA CCACAAGGCA 
GCCAAACAGA ACGTTAGGGG CCAGGAAGAC AACAAGGCCT GGAAGCTTAA GGCTGTGGAC 
GCCTTCAAGT CTGCCCCACT GTATCAGAGG CCAGGCTACT ACAGTGCCCC ACAGACGCCC 
CTCAGCCCCA CTCCCATGTT CTTCCCCCTA GAACCATCAG CGCCGTCAAA GCTTCACAGT 
GTCACAGGCA TAGACACCAA AGACAAAAGC TTAAAGACTG TGAGTTCTGG GGCCAAGAAA 
AGTTTTGAAT TGCTCTCAGA GAGCGATGGG GCCTTGATGG AGCACCCAGA AGTATCTCAA 
GTGAGGAGGA AAACTGTGGA GTTTAACCTG ACGGATATGC CAGAGATCCC CGAAAATCAC 
CTCAAAGAAC CTTTGGAACA ATCACCAACC AACATACACA CTACACTCAA AGATCACATG 
GATCCTTATT GGGCCTTGGA AAACAGGTCT GTCCTCCACC TGAACCAGGG GCACTGCATT 
GCCCTGTGCC CCACCCCAGC TTCCCTTGCT CTGAGCCTAC CCTTCCTCCA CAATTTCCTA 
GGGTTCCATC ACTGCCAGAG CACACTGGAC CTACGCCCAG CACTGGCTTG GGGTATATAC 
TTGGCCACCT TCACAGGGAT CCTAGGGAAG TGTTCGGGAC CTTTTCTCAC TTCACCCTGG 
TATCACCCGG AAGACTTCTT GGGACCAGGT GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_017018228.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873717.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu61311
Clone ID Related Accession (Same CDS sequence)	XM_017018228.1 , NM_001363591.1 , NM_001363591.2
Accession Version	XM_017018228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873717.1
CDS	664..2340
Translation	MSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_017018228.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018228.1

ORF Insert Sequence:

ATGAGCCTGG TGTCGGGCTT CGTCGAAGGC AAGGACGAGC AAGGCCGGCT GCTGCGGCGC 
ACGCTCATCC GCTACGCCAA CCTGGGCAAC GTGCTCATCC TGCGCAGCGT CAGCACCGCA 
GTCTACAAGC GCTTCCCCAG CGCCCAGCAC CTGGTGCAAG CAGGCTTTAT GACTCCGGCA 
GAACACAAGC AGTTGGAGAA ACTGAGCCTA CCACACAACA TGTTCTGGGT GCCCTGGGTG 
TGGTTTGCCA ACCTGTCAAT GAAGGCGTGG CTTGGAGGTC GAATCCGGGA CCCTATCCTG 
CTCCAGAGCC TGCTGAACGA GATGAACACC TTGCGTACTC AGTGTGGACA CCTGTATGCC 
TACGACTGGA TTAGTATCCC ACTGGTGTAT ACACAGGTGG TGACTGTGGC GGTGTACAGC 
TTCTTCCTGA CTTGTCTAGT TGGGCGGCAG TTTCTGAACC CAGCCAAGGC CTACCCTGGC 
CATGAGCTGG ACCTCGTTGT GCCCGTCTTC ACGTTCCTGC AGTTCTTCTT CTATGTTGGC 
TGGCTGAAGG TGGCAGAGCA GCTCATCAAC CCCTTTGGAG AGGATGATGA TGATTTTGAG 
ACCAACTGGA TTGTCGACAG GAATTTGCAG GTGTCCCTGT TGGCTGTGGA TGAGATGCAC 
CAGGACCTGC CTCGGATGGA GCCGGACATG TACTGGAATA AGCCCGAGCC ACAGCCCCCC 
TACACAGCTG CTTCCGCCCA GTTCCGTCGA GCCTCCTTTA TGGGCTCCAC CTTCAACATC 
AGCCTGAACA AAGAGGAGAT GGAGTTCCAG CCCAATCAGG AGGACGAGGA GGATGCTCAC 
GCTGGCATCA TTGGCCGCTT CCTAGGCCTG CAGTCCCATG ATCACCATCC TCCCAGGGCA 
AACTCAAGGA CCAAACTACT GTGGCCCAAG AGGGAATCCC TTCTCCACGA GGGCCTGCCC 
AAAAACCACA AGGCAGCCAA ACAGAACGTT AGGGGCCAGG AAGACAACAA GGCCTGGAAG 
CTTAAGGCTG TGGACGCCTT CAAGTCTGCC CCACTGTATC AGAGGCCAGG CTACTACAGT 
GCCCCACAGA CGCCCCTCAG CCCCACTCCC ATGTTCTTCC CCCTAGAACC ATCAGCGCCG 
TCAAAGCTTC ACAGTGTCAC AGGCATAGAC ACCAAAGACA AAAGCTTAAA GACTGTGAGT 
TCTGGGGCCA AGAAAAGTTT TGAATTGCTC TCAGAGAGCG ATGGGGCCTT GATGGAGCAC 
CCAGAAGTAT CTCAAGTGAG GAGGAAAACT GTGGAGTTTA ACCTGACGGA TATGCCAGAG 
ATCCCCGAAA ATCACCTCAA AGAACCTTTG GAACAATCAC CAACCAACAT ACACACTACA 
CTCAAAGATC ACATGGATCC TTATTGGGCC TTGGAAAACA GGTCTGTCCT CCACCTGAAC 
CAGGGGCACT GCATTGCCCT GTGCCCCACC CCAGCTTCCC TTGCTCTGAG CCTACCCTTC 
CTCCACAATT TCCTAGGGTT CCATCACTGC CAGAGCACAC TGGACCTACG CCCAGCACTG 
GCTTGGGGTA TATACTTGGC CACCTTCACA GGGATCCTAG GGAAGTGTTC GGGACCTTTT 
CTCACTTCAC CCTGGTATCA CCCGGAAGAC TTCTTGGGAC CAGGTGAAGG AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_017018229.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873718.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X6, mRNA.

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CloneID	OHu61312
Clone ID Related Accession (Same CDS sequence)	XM_017018229.1 , NM_001363592.1
Accession Version	XM_017018229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873718.1
CDS	691..1998
Translation	MTITYTSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLCYYIIRFIYRLALTEEQQLMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMSLVSGFVEGKDEQGRLLRRTLIRYANLGNVLILRSVSTAVYKRFPSAQHLVQAGFMTPAEHKQLEKLSLPHNMFWVPWVWFANLSMKAWLGGRIRDPILLQSLLNEMNTLRTQCGHLYAYDWISIPLVYTQVVTVAVYSFFLTCLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYVGWLKVGLSRALLGWRHGQRGHGQQLPETRMQCQERKVSRVESSQAWWRTPVIPATREAEAGESLEPGRRRLWWQSSSSTPLERMMMILRPTGLSTGICRCPCWLWMRCTRTCLGWSRTCTGISPSHSPPTQLLPPSSVEPPLWAPPSTSA

Target ORF information:

RefSeq Version	XM_017018229.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018229.1

ORF Insert Sequence:

ATGACCATCA CTTACACAAG CCAAGTGGCT AATGCCCGCT TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGGGGCAG CATCTACAAG CTGCTATATG GCGAGTTCTT AATCTTCCTG 
CTCTGCTACT ACATCATCCG CTTTATTTAT AGGCTGGCCC TCACGGAAGA ACAACAGCTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC GCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAGCCTGGTG TCGGGCTTCG TCGAAGGCAA GGACGAGCAA 
GGCCGGCTGC TGCGGCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCACCGCAGT CTACAAGCGC TTCCCCAGCG CCCAGCACCT GGTGCAAGCA 
GGCTTTATGA CTCCGGCAGA ACACAAGCAG TTGGAGAAAC TGAGCCTACC ACACAACATG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGCT TGGAGGTCGA 
ATCCGGGACC CTATCCTGCT CCAGAGCCTG CTGAACGAGA TGAACACCTT GCGTACTCAG 
TGTGGACACC TGTATGCCTA CGACTGGATT AGTATCCCAC TGGTGTATAC ACAGGTGGTG 
ACTGTGGCGG TGTACAGCTT CTTCCTGACT TGTCTAGTTG GGCGGCAGTT TCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA TGAGCTGGAC CTCGTTGTGC CCGTCTTCAC GTTCCTGCAG 
TTCTTCTTCT ATGTTGGCTG GCTGAAGGTG GGCCTCTCCA GGGCCCTGCT GGGCTGGAGG 
CATGGCCAGA GGGGTCATGG CCAGCAGCTG CCTGAGACGA GGATGCAGTG TCAGGAAAGG 
AAGGTCTCAC GGGTAGAAAG CAGCCAGGCG TGGTGGCGCA CACCTGTAAT CCCAGCTACT 
CGGGAGGCTG AGGCAGGAGA ATCGCTTGAA CCCGGGAGGC GGAGGTTGTG GTGGCAGAGC 
AGCTCATCAA CCCCTTTGGA GAGGATGATG ATGATTTTGA GACCAACTGG ATTGTCGACA 
GGAATTTGCA GGTGTCCCTG TTGGCTGTGG ATGAGATGCA CCAGGACCTG CCTCGGATGG 
AGCCGGACAT GTACTGGAAT AAGCCCGAGC CACAGCCCCC CTACACAGCT GCTTCCGCCC 
AGTTCCGTCG AGCCTCCTTT ATGGGCTCCA CCTTCAACAT CAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545233.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543535.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu61313
Clone ID Related Accession (Same CDS sequence)	XM_011545233.3 , XM_011545233.2
Accession Version	XM_011545233.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545233.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAA TTATGCAACT CCAGGTGGCA GAGCAGCTCA TCAACCCCTT TGGAGAGGAT 
GATGATGATT TTGAGACCAA CTGGATTGTC GACAGGAATT TGCAGGTGTC CCTGTTGGCT 
GTGGATGAGA TGCACCAGGA CCTGCCTCGG ATGGAGCCGG ACATGTACTG GAATAAGCCC 
GAGCCACAGC CCCCCTACAC AGCTGCTTCC GCCCAGTTCC GTCGAGCCTC CTTTATGGGC 
TCCACCTTCA ACATCAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA TCAGGAGGAC 
GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC CCATGATCAC 
CATCCTCCCA GGGCAAACTC AAGGACCAAA CTACTGTGGC CCAAGAGGGA ATCCCTTCTC 
CACGAGGGCC TGCCCAAAAA CCACAAGGCA GCCAAACAGA ACGTTAGGGG CCAGGAAGAC 
AACAAGGCCT GGAAGCTTAA GGCTGTGGAC GCCTTCAAGT CTGCCCCACT GTATCAGAGG 
CCAGGCTACT ACAGTGCCCC ACAGACGCCC CTCAGCCCCA CTCCCATGTT CTTCCCCCTA 
GAACCATCAG CGCCGTCAAA GCTTCACAGT GTCACAGGCA TAGACACCAA AGACAAAAGC 
TTAAAGACTG TGAGTTCTGG GGCCAAGAAA AGTTTTGAAT TGCTCTCAGA GAGCGATGGG 
GCCTTGATGG AGCACCCAGA AGTATCTCAA GTGAGGAGGA AAACTGTGGA GTTTAACCTG 
ACGGATATGC CAGAGATCCC CGAAAATCAC CTCAAAGAAC CTTTGGAACA ATCACCAACC 
AACATACACA CTACACTCAA AGATCACATG GATCCTTATT GGGCCTTGGA AAACAGGTCT 
GTCCTCCACC TGAACCAGGG GCACTGCATT GCCCTGTGCC CCACCCCAGC TTCCCTTGCT 
CTGAGCCTAC CCTTCCTCCA CAATTTCCTA GGGTTCCATC ACTGCCAGAG CACACTGGAC 
CTACGCCCAG CACTGGCTTG GGGTATATAC TTGGCCACCT TCACAGGGAT CCTAGGGAAG 
TGTTCGGGAC CTTTTCTCAC TTCACCCTGG TATCACCCGG AAGACTTCTT GGGACCAGGT 
GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543535.1
CDS	671..1822
Translation	MNKIMQLQVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545233.3
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545233.3

ORF Insert Sequence:

ATGAATAAAA TTATGCAACT CCAGGTGGCA GAGCAGCTCA TCAACCCCTT TGGAGAGGAT 
GATGATGATT TTGAGACCAA CTGGATTGTC GACAGGAATT TGCAGGTGTC CCTGTTGGCT 
GTGGATGAGA TGCACCAGGA CCTGCCTCGG ATGGAGCCGG ACATGTACTG GAATAAGCCC 
GAGCCACAGC CCCCCTACAC AGCTGCTTCC GCCCAGTTCC GTCGAGCCTC CTTTATGGGC 
TCCACCTTCA ACATCAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA TCAGGAGGAC 
GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC CCATGATCAC 
CATCCTCCCA GGGCAAACTC AAGGACCAAA CTACTGTGGC CCAAGAGGGA ATCCCTTCTC 
CACGAGGGCC TGCCCAAAAA CCACAAGGCA GCCAAACAGA ACGTTAGGGG CCAGGAAGAC 
AACAAGGCCT GGAAGCTTAA GGCTGTGGAC GCCTTCAAGT CTGCCCCACT GTATCAGAGG 
CCAGGCTACT ACAGTGCCCC ACAGACGCCC CTCAGCCCCA CTCCCATGTT CTTCCCCCTA 
GAACCATCAG CGCCGTCAAA GCTTCACAGT GTCACAGGCA TAGACACCAA AGACAAAAGC 
TTAAAGACTG TGAGTTCTGG GGCCAAGAAA AGTTTTGAAT TGCTCTCAGA GAGCGATGGG 
GCCTTGATGG AGCACCCAGA AGTATCTCAA GTGAGGAGGA AAACTGTGGA GTTTAACCTG 
ACGGATATGC CAGAGATCCC CGAAAATCAC CTCAAAGAAC CTTTGGAACA ATCACCAACC 
AACATACACA CTACACTCAA AGATCACATG GATCCTTATT GGGCCTTGGA AAACAGGTCT 
GTCCTCCACC TGAACCAGGG GCACTGCATT GCCCTGTGCC CCACCCCAGC TTCCCTTGCT 
CTGAGCCTAC CCTTCCTCCA CAATTTCCTA GGGTTCCATC ACTGCCAGAG CACACTGGAC 
CTACGCCCAG CACTGGCTTG GGGTATATAC TTGGCCACCT TCACAGGGAT CCTAGGGAAG 
TGTTCGGGAC CTTTTCTCAC TTCACCCTGG TATCACCCGG AAGACTTCTT GGGACCAGGT 
GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_011545233.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543535.1 Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu61313
Clone ID Related Accession (Same CDS sequence)	XM_011545233.3 , XM_011545233.2
Accession Version	XM_011545233.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAA TTATGCAACT CCAGGTGGCA GAGCAGCTCA TCAACCCCTT TGGAGAGGAT 
GATGATGATT TTGAGACCAA CTGGATTGTC GACAGGAATT TGCAGGTGTC CCTGTTGGCT 
GTGGATGAGA TGCACCAGGA CCTGCCTCGG ATGGAGCCGG ACATGTACTG GAATAAGCCC 
GAGCCACAGC CCCCCTACAC AGCTGCTTCC GCCCAGTTCC GTCGAGCCTC CTTTATGGGC 
TCCACCTTCA ACATCAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA TCAGGAGGAC 
GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC CCATGATCAC 
CATCCTCCCA GGGCAAACTC AAGGACCAAA CTACTGTGGC CCAAGAGGGA ATCCCTTCTC 
CACGAGGGCC TGCCCAAAAA CCACAAGGCA GCCAAACAGA ACGTTAGGGG CCAGGAAGAC 
AACAAGGCCT GGAAGCTTAA GGCTGTGGAC GCCTTCAAGT CTGCCCCACT GTATCAGAGG 
CCAGGCTACT ACAGTGCCCC ACAGACGCCC CTCAGCCCCA CTCCCATGTT CTTCCCCCTA 
GAACCATCAG CGCCGTCAAA GCTTCACAGT GTCACAGGCA TAGACACCAA AGACAAAAGC 
TTAAAGACTG TGAGTTCTGG GGCCAAGAAA AGTTTTGAAT TGCTCTCAGA GAGCGATGGG 
GCCTTGATGG AGCACCCAGA AGTATCTCAA GTGAGGAGGA AAACTGTGGA GTTTAACCTG 
ACGGATATGC CAGAGATCCC CGAAAATCAC CTCAAAGAAC CTTTGGAACA ATCACCAACC 
AACATACACA CTACACTCAA AGATCACATG GATCCTTATT GGGCCTTGGA AAACAGGTCT 
GTCCTCCACC TGAACCAGGG GCACTGCATT GCCCTGTGCC CCACCCCAGC TTCCCTTGCT 
CTGAGCCTAC CCTTCCTCCA CAATTTCCTA GGGTTCCATC ACTGCCAGAG CACACTGGAC 
CTACGCCCAG CACTGGCTTG GGGTATATAC TTGGCCACCT TCACAGGGAT CCTAGGGAAG 
TGTTCGGGAC CTTTTCTCAC TTCACCCTGG TATCACCCGG AAGACTTCTT GGGACCAGGT 
GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543535.1
CDS	671..1822
Translation	MNKIMQLQVAEQLINPFGEDDDDFETNWIVDRNLQVSLLAVDEMHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	XM_011545233.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545233.2

ORF Insert Sequence:

ATGAATAAAA TTATGCAACT CCAGGTGGCA GAGCAGCTCA TCAACCCCTT TGGAGAGGAT 
GATGATGATT TTGAGACCAA CTGGATTGTC GACAGGAATT TGCAGGTGTC CCTGTTGGCT 
GTGGATGAGA TGCACCAGGA CCTGCCTCGG ATGGAGCCGG ACATGTACTG GAATAAGCCC 
GAGCCACAGC CCCCCTACAC AGCTGCTTCC GCCCAGTTCC GTCGAGCCTC CTTTATGGGC 
TCCACCTTCA ACATCAGCCT GAACAAAGAG GAGATGGAGT TCCAGCCCAA TCAGGAGGAC 
GAGGAGGATG CTCACGCTGG CATCATTGGC CGCTTCCTAG GCCTGCAGTC CCATGATCAC 
CATCCTCCCA GGGCAAACTC AAGGACCAAA CTACTGTGGC CCAAGAGGGA ATCCCTTCTC 
CACGAGGGCC TGCCCAAAAA CCACAAGGCA GCCAAACAGA ACGTTAGGGG CCAGGAAGAC 
AACAAGGCCT GGAAGCTTAA GGCTGTGGAC GCCTTCAAGT CTGCCCCACT GTATCAGAGG 
CCAGGCTACT ACAGTGCCCC ACAGACGCCC CTCAGCCCCA CTCCCATGTT CTTCCCCCTA 
GAACCATCAG CGCCGTCAAA GCTTCACAGT GTCACAGGCA TAGACACCAA AGACAAAAGC 
TTAAAGACTG TGAGTTCTGG GGCCAAGAAA AGTTTTGAAT TGCTCTCAGA GAGCGATGGG 
GCCTTGATGG AGCACCCAGA AGTATCTCAA GTGAGGAGGA AAACTGTGGA GTTTAACCTG 
ACGGATATGC CAGAGATCCC CGAAAATCAC CTCAAAGAAC CTTTGGAACA ATCACCAACC 
AACATACACA CTACACTCAA AGATCACATG GATCCTTATT GGGCCTTGGA AAACAGGTCT 
GTCCTCCACC TGAACCAGGG GCACTGCATT GCCCTGTGCC CCACCCCAGC TTCCCTTGCT 
CTGAGCCTAC CCTTCCTCCA CAATTTCCTA GGGTTCCATC ACTGCCAGAG CACACTGGAC 
CTACGCCCAG CACTGGCTTG GGGTATATAC TTGGCCACCT TCACAGGGAT CCTAGGGAAG 
TGTTCGGGAC CTTTTCTCAC TTCACCCTGG TATCACCCGG AAGACTTCTT GGGACCAGGT 
GAAGGAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001363593.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350522.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu107873
Clone ID Related Accession (Same CDS sequence)	NM_001363593.2 , NM_001363593.1
Accession Version	NM_001363593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003733.5. On May 31, 2019 this sequence version replaced NM_001363593.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.110792.1, SRR3476690.120773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCACCAGG ACCTGCCTCG GATGGAGCCG GACATGTACT GGAATAAGCC CGAGCCACAG 
CCCCCCTACA CAGCTGCTTC CGCCCAGTTC CGTCGAGCCT CCTTTATGGG CTCCACCTTC 
AACATCAGCC TGAACAAAGA GGAGATGGAG TTCCAGCCCA ATCAGGAGGA CGAGGAGGAT 
GCTCACGCTG GCATCATTGG CCGCTTCCTA GGCCTGCAGT CCCATGATCA CCATCCTCCC 
AGGGCAAACT CAAGGACCAA ACTACTGTGG CCCAAGAGGG AATCCCTTCT CCACGAGGGC 
CTGCCCAAAA ACCACAAGGC AGCCAAACAG AACGTTAGGG GCCAGGAAGA CAACAAGGCC 
TGGAAGCTTA AGGCTGTGGA CGCCTTCAAG TCTGCCCCAC TGTATCAGAG GCCAGGCTAC 
TACAGTGCCC CACAGACGCC CCTCAGCCCC ACTCCCATGT TCTTCCCCCT AGAACCATCA 
GCGCCGTCAA AGCTTCACAG TGTCACAGGC ATAGACACCA AAGACAAAAG CTTAAAGACT 
GTGAGTTCTG GGGCCAAGAA AAGTTTTGAA TTGCTCTCAG AGAGCGATGG GGCCTTGATG 
GAGCACCCAG AAGTATCTCA AGTGAGGAGG AAAACTGTGG AGTTTAACCT GACGGATATG 
CCAGAGATCC CCGAAAATCA CCTCAAAGAA CCTTTGGAAC AATCACCAAC CAACATACAC 
ACTACACTCA AAGATCACAT GGATCCTTAT TGGGCCTTGG AAAACAGGTC TGTCCTCCAC 
CTGAACCAGG GGCACTGCAT TGCCCTGTGC CCCACCCCAG CTTCCCTTGC TCTGAGCCTA 
CCCTTCCTCC ACAATTTCCT AGGGTTCCAT CACTGCCAGA GCACACTGGA CCTACGCCCA 
GCACTGGCTT GGGGTATATA CTTGGCCACC TTCACAGGGA TCCTAGGGAA GTGTTCGGGA 
CCTTTTCTCA CTTCACCCTG GTATCACCCG GAAGACTTCT TGGGACCAGG TGAAGGAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350522.1
CDS	1082..2104
Translation	MHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001363593.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363593.2

ORF Insert Sequence:

ATGCACCAGG ACCTGCCTCG GATGGAGCCG GACATGTACT GGAATAAGCC CGAGCCACAG 
CCCCCCTACA CAGCTGCTTC CGCCCAGTTC CGTCGAGCCT CCTTTATGGG CTCCACCTTC 
AACATCAGCC TGAACAAAGA GGAGATGGAG TTCCAGCCCA ATCAGGAGGA CGAGGAGGAT 
GCTCACGCTG GCATCATTGG CCGCTTCCTA GGCCTGCAGT CCCATGATCA CCATCCTCCC 
AGGGCAAACT CAAGGACCAA ACTACTGTGG CCCAAGAGGG AATCCCTTCT CCACGAGGGC 
CTGCCCAAAA ACCACAAGGC AGCCAAACAG AACGTTAGGG GCCAGGAAGA CAACAAGGCC 
TGGAAGCTTA AGGCTGTGGA CGCCTTCAAG TCTGCCCCAC TGTATCAGAG GCCAGGCTAC 
TACAGTGCCC CACAGACGCC CCTCAGCCCC ACTCCCATGT TCTTCCCCCT AGAACCATCA 
GCGCCGTCAA AGCTTCACAG TGTCACAGGC ATAGACACCA AAGACAAAAG CTTAAAGACT 
GTGAGTTCTG GGGCCAAGAA AAGTTTTGAA TTGCTCTCAG AGAGCGATGG GGCCTTGATG 
GAGCACCCAG AAGTATCTCA AGTGAGGAGG AAAACTGTGG AGTTTAACCT GACGGATATG 
CCAGAGATCC CCGAAAATCA CCTCAAAGAA CCTTTGGAAC AATCACCAAC CAACATACAC 
ACTACACTCA AAGATCACAT GGATCCTTAT TGGGCCTTGG AAAACAGGTC TGTCCTCCAC 
CTGAACCAGG GGCACTGCAT TGCCCTGTGC CCCACCCCAG CTTCCCTTGC TCTGAGCCTA 
CCCTTCCTCC ACAATTTCCT AGGGTTCCAT CACTGCCAGA GCACACTGGA CCTACGCCCA 
GCACTGGCTT GGGGTATATA CTTGGCCACC TTCACAGGGA TCCTAGGGAA GTGTTCGGGA 
CCTTTTCTCA CTTCACCCTG GTATCACCCG GAAGACTTCT TGGGACCAGG TGAAGGAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( NM_001363593.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350522.1 Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu107873
Clone ID Related Accession (Same CDS sequence)	NM_001363593.2 , NM_001363593.1
Accession Version	NM_001363593.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	bestrophin-1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003733.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.110792.1, SRR3476690.120773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCACCAGG ACCTGCCTCG GATGGAGCCG GACATGTACT GGAATAAGCC CGAGCCACAG 
CCCCCCTACA CAGCTGCTTC CGCCCAGTTC CGTCGAGCCT CCTTTATGGG CTCCACCTTC 
AACATCAGCC TGAACAAAGA GGAGATGGAG TTCCAGCCCA ATCAGGAGGA CGAGGAGGAT 
GCTCACGCTG GCATCATTGG CCGCTTCCTA GGCCTGCAGT CCCATGATCA CCATCCTCCC 
AGGGCAAACT CAAGGACCAA ACTACTGTGG CCCAAGAGGG AATCCCTTCT CCACGAGGGC 
CTGCCCAAAA ACCACAAGGC AGCCAAACAG AACGTTAGGG GCCAGGAAGA CAACAAGGCC 
TGGAAGCTTA AGGCTGTGGA CGCCTTCAAG TCTGCCCCAC TGTATCAGAG GCCAGGCTAC 
TACAGTGCCC CACAGACGCC CCTCAGCCCC ACTCCCATGT TCTTCCCCCT AGAACCATCA 
GCGCCGTCAA AGCTTCACAG TGTCACAGGC ATAGACACCA AAGACAAAAG CTTAAAGACT 
GTGAGTTCTG GGGCCAAGAA AAGTTTTGAA TTGCTCTCAG AGAGCGATGG GGCCTTGATG 
GAGCACCCAG AAGTATCTCA AGTGAGGAGG AAAACTGTGG AGTTTAACCT GACGGATATG 
CCAGAGATCC CCGAAAATCA CCTCAAAGAA CCTTTGGAAC AATCACCAAC CAACATACAC 
ACTACACTCA AAGATCACAT GGATCCTTAT TGGGCCTTGG AAAACAGGTC TGTCCTCCAC 
CTGAACCAGG GGCACTGCAT TGCCCTGTGC CCCACCCCAG CTTCCCTTGC TCTGAGCCTA 
CCCTTCCTCC ACAATTTCCT AGGGTTCCAT CACTGCCAGA GCACACTGGA CCTACGCCCA 
GCACTGGCTT GGGGTATATA CTTGGCCACC TTCACAGGGA TCCTAGGGAA GTGTTCGGGA 
CCTTTTCTCA CTTCACCCTG GTATCACCCG GAAGACTTCT TGGGACCAGG TGAAGGAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350522.1
CDS	1521..2543
Translation	MHQDLPRMEPDMYWNKPEPQPPYTAASAQFRRASFMGSTFNISLNKEEMEFQPNQEDEEDAHAGIIGRFLGLQSHDHHPPRANSRTKLLWPKRESLLHEGLPKNHKAAKQNVRGQEDNKAWKLKAVDAFKSAPLYQRPGYYSAPQTPLSPTPMFFPLEPSAPSKLHSVTGIDTKDKSLKTVSSGAKKSFELLSESDGALMEHPEVSQVRRKTVEFNLTDMPEIPENHLKEPLEQSPTNIHTTLKDHMDPYWALENRSVLHLNQGHCIALCPTPASLALSLPFLHNFLGFHHCQSTLDLRPALAWGIYLATFTGILGKCSGPFLTSPWYHPEDFLGPGEGR

Target ORF information:

RefSeq Version	NM_001363593.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bestrophin 1 (BEST1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363593.1

ORF Insert Sequence:

ATGCACCAGG ACCTGCCTCG GATGGAGCCG GACATGTACT GGAATAAGCC CGAGCCACAG 
CCCCCCTACA CAGCTGCTTC CGCCCAGTTC CGTCGAGCCT CCTTTATGGG CTCCACCTTC 
AACATCAGCC TGAACAAAGA GGAGATGGAG TTCCAGCCCA ATCAGGAGGA CGAGGAGGAT 
GCTCACGCTG GCATCATTGG CCGCTTCCTA GGCCTGCAGT CCCATGATCA CCATCCTCCC 
AGGGCAAACT CAAGGACCAA ACTACTGTGG CCCAAGAGGG AATCCCTTCT CCACGAGGGC 
CTGCCCAAAA ACCACAAGGC AGCCAAACAG AACGTTAGGG GCCAGGAAGA CAACAAGGCC 
TGGAAGCTTA AGGCTGTGGA CGCCTTCAAG TCTGCCCCAC TGTATCAGAG GCCAGGCTAC 
TACAGTGCCC CACAGACGCC CCTCAGCCCC ACTCCCATGT TCTTCCCCCT AGAACCATCA 
GCGCCGTCAA AGCTTCACAG TGTCACAGGC ATAGACACCA AAGACAAAAG CTTAAAGACT 
GTGAGTTCTG GGGCCAAGAA AAGTTTTGAA TTGCTCTCAG AGAGCGATGG GGCCTTGATG 
GAGCACCCAG AAGTATCTCA AGTGAGGAGG AAAACTGTGG AGTTTAACCT GACGGATATG 
CCAGAGATCC CCGAAAATCA CCTCAAAGAA CCTTTGGAAC AATCACCAAC CAACATACAC 
ACTACACTCA AAGATCACAT GGATCCTTAT TGGGCCTTGG AAAACAGGTC TGTCCTCCAC 
CTGAACCAGG GGCACTGCAT TGCCCTGTGC CCCACCCCAG CTTCCCTTGC TCTGAGCCTA 
CCCTTCCTCC ACAATTTCCT AGGGTTCCAT CACTGCCAGA GCACACTGGA CCTACGCCCA 
GCACTGGCTT GGGGTATATA CTTGGCCACC TTCACAGGGA TCCTAGGGAA GTGTTCGGGA 
CCTTTTCTCA CTTCACCCTG GTATCACCCG GAAGACTTCT TGGGACCAGG TGAAGGAAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variations in Bestrophin?1 and associated clinical findings in two Chinese patients with juvenile?onset and adult?onset best vitelliform macular dystrophy.
Molecular medicine reports17(1)225-233(2018 Jan)
Lin Y,Li T,Ma C,Gao H,Chen C,Zhu Y,Liu B,Lian Y,Huang Y,Li H,Wu Q,Liang X,Jin C,Huang X,Ye J,Lu L

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BEST1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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