VCL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	7414
Full Name	vinculin
Synonyms	CMD1W,CMH15,HEL114,MV,MVCL
General protein information	Preferred Names vinculin Names vinculin epididymis luminal protein 114 meta-vinculin metavinculin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.2
Summary	Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	193065
Disorder Html:	Cardiomyopathy, dilated, 1W, 611407 (3); Cardiomyopathy, hypertrophic, 15, 613255 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_014000.2	NP_054706.1	vinculin isoform meta-VCL
NM_003373.4	NP_003364.1	vinculin isoform VCL
NM_014000.3	NP_054706.1	vinculin isoform meta-VCL
NM_003373.3	NP_003364.1	vinculin isoform VCL

Pathways from BioSystems

Homologies

	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology
Bibliography

Related articles in PubMed

Vinculin is required to maintain glomerular barrier integrity.
Lausecker F, Tian X, Inoue K, Wang Z, Pedigo CE, Hassan H, Liu C, Zimmer M, Jinno S, Huckle AL, Hamidi H, Ross RS, Zent R, Ballestrem C, Lennon R, Ishibe S
Kidney international(2017 Dec)

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Rogg M, Yasuda-Yamahara M, Abed A, Dinse P, Helmst?dter M, Conzelmann AC, Frimmel J, Sellung D, Biniossek ML, Kretz O, Grahammer F, Schilling O, Huber TB, Schell C
Scientific reports7(1)15910(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study used an all-heavy-atom structure-based model to study vinculin activation by talin in a high-tension context mechanically driven by F-actin, showed that vinculin activation may proceed from an intermediate state stabilized by partial talin-vinculin association. There is a low-force regime and a high-force regime where vinculin activation is dominated by two different pathways with distinct responses to force.
Title: Molecular Simulations Suggest a Force-Dependent Mechanism of Vinculin Activation.

This review discusses the current understanding of the roles of vinculin in cell-cell and cell-matrix adhesions. Emphasis is placed on the how vinculin is recruited, activated and regulated. [review]
Title: Vinculin in cell-cell and cell-matrix adhesions.

We identified mutations in VCL associated with Short segment Hirschsprung disease. Correction of this mutation in induced pluripotent stem cells using CRISPR/Cas9 editing, as well as the RET G731del mutation that causes Hirschsprung disease with total colonic aganglionosis, restored enteric neural crest cell function.
Title: Correction of Hirschsprung-Associated Mutations in Human Induced Pluripotent Stem Cells Via Clustered Regularly Interspaced Short Palindromic Repeats/Cas9, Restores Neural?Crest Cell Function.

Study predicted the structure of the MAPK1-vinculin binding interface using a combination of flexible docking and molecular dynamics simulations, and confirmed that the MAPK1-vinculin interaction is mechanically regulated, and implicated a change in the vinculin D3-D4 cleft size upon vinculin activation as the basis for the conformational selectivity of MAPK1 binding toward open vinculin.
Title: Mechanosensitive Conformation of Vinculin Regulates Its Binding to MAPK1.

East Asian common VCL variant p.Asp841His (D841H) was associated with sudden unexplained nocturnal death syndrome (SUNDS) in the Chinese Han population.
Title: An East Asian Common Variant Vinculin P.Asp841His Was Associated With Sudden Unexplained Nocturnal Death Syndrome in the Chinese Han Population.

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25331	NM_003373.4 Latest version!	Homo sapiens vinculin (VCL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25616	NM_014000.3 Latest version!	Homo sapiens vinculin (VCL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
View Old Accession Versions >>
OHu25616	NM_014000.2	Homo sapiens vinculin (VCL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu25331	NM_003373.3	Homo sapiens vinculin (VCL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( NM_003373.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003364.1 Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25331
Clone ID Related Accession (Same CDS sequence)	NM_003373.4 , NM_003373.3
Accession Version	NM_003373.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	vinculin isoform VCL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, BX537994.1, AK126978.1, BC013847.1, AI290052.1, AL832130.1 and AL731576.9. On Jun 2, 2019 this sequence version replaced NM_003373.3. Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.178379.1, SRR1660807.248162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003364.1
CDS	93..3293
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_003373.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003373.4

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( NM_003373.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003364.1 Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25331
Clone ID Related Accession (Same CDS sequence)	NM_003373.4 , NM_003373.3
Accession Version	NM_003373.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	vinculin isoform VCL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, BX537994.1, AK126978.1, BC013847.1, AI290052.1 and AL832130.1. On Jul 26, 2004 this sequence version replaced NM_003373.2. Transcript Variant: This variant (2) lacks a 3' coding exon, as compared to variant 1. It encodes a shorter isoform (VCL), which is the major talin-binding protein in platelets. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.3834.1, SRR1803611.74744.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003364.1
CDS	95..3295
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_003373.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vinculin (VCL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003373.3

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCTGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT CATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAACGGAT TAGAACCAAC 
CTCTTACAGG TATGTGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTGCGGGAA 
GCTGAAGCTG CTTCAATCAA AATTCGAACA GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( NM_014000.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_054706.1 Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

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CloneID	OHu25616
Clone ID Related Accession (Same CDS sequence)	NM_014000.2 , NM_014000.3
Accession Version	NM_014000.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	vinculin isoform meta-VCL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, AK126978.1, AL832130.1, AA368699.1, AL700886.1, BC013847.1 and AI290052.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2004 this sequence version replaced NM_014000.1. Transcript Variant: This variant (1) differs from variant 2 in having an additional exon in the 3' coding region. It thus encodes a longer isoform (meta-VCL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794673.1, SRR1803612.133086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054706.1
CDS	95..3499
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_014000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014000.2

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( NM_014000.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054706.1 Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

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CloneID	OHu25616
Clone ID Related Accession (Same CDS sequence)	NM_014000.2 , NM_014000.3
Accession Version	NM_014000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	vinculin isoform meta-VCL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL708715.1, BC039174.1, AK126978.1, AL832130.1, AA368699.1, AL700886.1, BC013847.1, AI290052.1 and AL731576.9. On Nov 22, 2019 this sequence version replaced NM_014000.2. Transcript Variant: This variant (1) differs from variant 2 in having an additional exon in the 3' coding region. It thus encodes a longer isoform (meta-VCL). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794673.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054706.1
CDS	93..3497
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_014000.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vinculin (VCL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014000.3

ORF Insert Sequence:

ATGCCAGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC ACAGCAGATC 
TCCCACCTGG TGATAATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCCGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGCACCAA GCTTGTCCAG GCAGCTCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GCATCCTCTC TGGAACATCA 
GACCTGCTCC TTACCTTCGA TGAGGCTGAG GTCCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTGGAGACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGGCCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGACAGCAG 
GAGCTCACTC ACCAGGAGCA CCGAGTGATG TTGGTGAACT CGATGAACAC CGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGCATAG AGGAAGCTTT AAAAAATCGC AATTTTACTG TAGAAAAAAT GAGTGCTGAA 
ATTAATGAGA TAATTCGTGT GTTACAACTC ACCTCTTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC TAGTGCCTCC CCAGGGGATG CTGGTGAGCA GGCCATCAGA 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATTCTGGGAA CTTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGTGCC 
AGAGGACAAG GATCCTCACC GGTGGCCATG CAGAAAGCTC AGCAGGTATC TCAGGGTCTG 
GATGTGCTCA CAGCAAAAGT GGAAAATGCA GCTCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAACT GGCTTGCAGA TCCAAATGGT 
GGACCGGAAG GAGAAGAGCA GATTCGAGGT GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA AAGAGATGAC ATTCTACGTT CCCTTGGGGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGGAAAG GAGATTCTCC AGAGGCTCGA 
GCCTTGGCCA AACAGGTGGC CACGGCCCTG CAGAACCTGC AGACCAAAAC CAACCGGGCT 
GTGGCCAACA GCAGACCGGC CAAAGCAGCT GTACACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGTGGAG TCGGTCAGGC TGCCATCCGG 
GGGCTTGTGG CCGAAGGGCA TCGTCTGGCT AATGTTATGA TGGGGCCTTA TCGGCAAGAT 
CTTCTCGCCA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCACGA GCACTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTAA AAGCTCGGAT GCAGGAGGCC ATGACTCAGG AAGTGTCAGA TGTTTTCAGC 
GATACCACAA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CGGCGCCTCC TGATGCGCCT 
AACAGGGAAG AGGTATTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAAGCTT 
GGTGCTACGG CCGAGAAGGC GGCTGCGGTT GGTACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC GGCCCGAGAA CTCACACCCC AGGTGGTCTC GGCTGCTCGT 
ATCTTACTTA GGAACCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCAGA AGAAGCAATT AAAAAAGACC TGGACAAGTG CAAGGTAGCT 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAATT CCGAGGATCC CAAGTTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCGAT GGTGATGGAT 
GCAAAAGCTG TGGCTGGAAA CATTTCCGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CGCCTCCACC AGACCTTGAA CAACTCCGAC TAACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA AGGTGAGGTC CCTCCACCTA GGCCTCCACC ACCAGAGGAA 
AAGGATGAAG AGTTCCCTGA GCAGAAGGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGAC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GCAAGCCGGG CATCCCAGCC 
GCTGAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CCGATGCTGC TGGCTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCTGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTCATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAAC GGATTAGAAC CAACCTCTTA CAGGTATGTG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTGCG GGAAGCTGAA GCTGCTTCAA TCAAAATTCG AACAGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vinculin is required to maintain glomerular barrier integrity.
Kidney international(2017 Dec)
Lausecker F,Tian X,Inoue K,Wang Z,Pedigo CE,Hassan H,Liu C,Zimmer M,Jinno S,Huckle AL,Hamidi H,Ross RS,Zent R,Ballestrem C,Lennon R,Ishibe S

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Scientific reports7(1)15910(2017 Nov)
Rogg M,Yasuda-Yamahara M,Abed A,Dinse P,Helmst?dter M,Conzelmann AC,Frimmel J,Sellung D,Biniossek ML,Kretz O,Grahammer F,Schilling O,Huber TB,Schell C

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VCL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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