KDM6A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KDM6A
Entrez Gene ID	7403
Full Name	lysine demethylase 6A
Synonyms	KABUK2,UTX,bA386N14.2
General protein information	Preferred Names lysine demethylase 6A Names lysine-specific demethylase 6A bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)) histone demethylase UTX lysine (K)-specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat protein X-linked ubiquitously-transcribed TPR gene on the X chromosome
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.3
Summary	This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014].
Disorder MIM:	300128
Disorder Html:	Kabuki syndrome 2, 300867 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011543973.2	XP_011542275.1	lysine-specific demethylase 6A isoform X19
XM_011543969.2	XP_011542271.1	lysine-specific demethylase 6A isoform X15
XM_011543971.2	XP_011542273.1	lysine-specific demethylase 6A isoform X17
XM_011543963.2	XP_011542265.1	lysine-specific demethylase 6A isoform X7
XM_011543964.2	XP_011542266.1	lysine-specific demethylase 6A isoform X8
XM_024452438.1	XP_024308206.1	lysine-specific demethylase 6A isoform X2
XM_011543974.2	XP_011542276.1	lysine-specific demethylase 6A isoform X12
XM_011543975.2	XP_011542277.1	lysine-specific demethylase 6A isoform X13
XM_017029784.1	XP_016885273.1	lysine-specific demethylase 6A isoform X14
XM_017029785.1	XP_016885274.1	lysine-specific demethylase 6A isoform X15
XM_011543962.2	XP_011542264.1	lysine-specific demethylase 6A isoform X6
NM_001291421.1	NP_001278350.1	lysine-specific demethylase 6A isoform 6
NM_021140.3	NP_066963.2	lysine-specific demethylase 6A isoform 3
NM_001291415.2	NP_001278344.1	lysine-specific demethylase 6A isoform 1
XM_005272656.5	XP_005272713.1	lysine-specific demethylase 6A isoform X5
XM_017029783.2	XP_016885272.1	lysine-specific demethylase 6A isoform X9
XM_024452439.1	XP_024308207.1	lysine-specific demethylase 6A isoform X11
XM_011543957.2	XP_011542259.1	lysine-specific demethylase 6A isoform X1
XM_011543958.2	XP_011542260.1	lysine-specific demethylase 6A isoform X2
XM_011543960.2	XP_011542262.1	lysine-specific demethylase 6A isoform X4
XM_011543970.2	XP_011542272.1	lysine-specific demethylase 6A isoform X16
XM_005272659.4	XP_005272716.1	lysine-specific demethylase 6A isoform X21
XM_011543974.1	XP_011542276.1	lysine-specific demethylase 6A isoform X22
NM_001291415.1	NP_001278344.1	lysine-specific demethylase 6A isoform 1
XM_011543958.3	XP_011542260.1	lysine-specific demethylase 6A isoform X1
XM_011543963.3	XP_011542265.1	lysine-specific demethylase 6A isoform X3
XM_005272659.5	XP_005272716.1	lysine-specific demethylase 6A isoform X10
XM_017029782.1	XP_016885271.1	lysine-specific demethylase 6A isoform X10
XM_011543966.2	XP_011542268.1	lysine-specific demethylase 6A isoform X11
XM_011543968.2	XP_011542270.1	lysine-specific demethylase 6A isoform X14
XM_011543972.2	XP_011542274.1	lysine-specific demethylase 6A isoform X18
NM_001291417.1	NP_001278346.1	lysine-specific demethylase 6A isoform 4
XM_011543969.3	XP_011542271.1	lysine-specific demethylase 6A isoform X6
XM_011543972.3	XP_011542274.1	lysine-specific demethylase 6A isoform X8
XM_011543959.2	XP_011542261.1	lysine-specific demethylase 6A isoform X3
XM_011543961.2	XP_011542263.1	lysine-specific demethylase 6A isoform X5
XM_011543965.2	XP_011542267.1	lysine-specific demethylase 6A isoform X9
XM_011543967.2	XP_011542269.1	lysine-specific demethylase 6A isoform X12
XM_005272656.4	XP_005272713.1	lysine-specific demethylase 6A isoform X13
XM_017029783.1	XP_016885272.1	lysine-specific demethylase 6A isoform X20
NM_001291416.1	NP_001278345.1	lysine-specific demethylase 6A isoform 2
NM_001291418.1	NP_001278347.1	lysine-specific demethylase 6A isoform 5
XM_011543964.3	XP_011542266.1	lysine-specific demethylase 6A isoform X4
XM_011543970.3	XP_011542272.1	lysine-specific demethylase 6A isoform X7

Pathways from BioSystems

Homologies

Homo sapiens (human)	KDM6A	NP_066963.2
Danio rerio (zebrafish)	kdm6a	XP_697746.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008509	XP_316935.4
Xenopus tropicalis (tropical clawed frog)	kdm6a	NP_001123807.1
Bos taurus (cattle)	KDM6A	NP_001193504.1
Drosophila melanogaster (fruit fly)	Utx	NP_001188773.1
Pan troglodytes (chimpanzee)	KDM6A	XP_003954022.1
Canis lupus familiaris (dog)	KDM6A	NP_001184114.1
Caenorhabditis elegans (roundworm)	utx-1	NP_509450.1
Rattus norvegicus (Norway rat)	Kdm6a	XP_006256701.1
	KDM6A	XP_002806253.1
Mus musculus (house mouse)	Kdm6a	NP_033509.1
Gallus gallus (chicken)	KDM6A	XP_416762.4

Gene Ontology

The following KDM6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24583	NM_021140.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu24601	NM_001291415.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu106278	XM_024452438.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu106279	XM_024452439.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu99447	XM_017029782.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu99448	XM_017029783.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu99449	XM_017029784.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu99450	XM_017029785.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu24600	NM_001291421.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu24605	NM_001291418.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61265	XM_011543974.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu61266	XM_011543975.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu24587	NM_001291417.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu24588	NM_001291416.1 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54022	XM_005272656.5 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54024	XM_005272659.5 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61248	XM_011543957.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu61249	XM_011543958.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61250	XM_011543959.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61251	XM_011543960.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61252	XM_011543961.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61253	XM_011543962.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61254	XM_011543963.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61255	XM_011543964.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61256	XM_011543965.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61257	XM_011543966.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61258	XM_011543967.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61259	XM_011543968.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61260	XM_011543969.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61261	XM_011543970.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61262	XM_011543971.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61263	XM_011543972.3 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61264	XM_011543973.2 Latest version!	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu61265	XM_011543974.1	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu24601	NM_001291415.1	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61249	XM_011543958.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61254	XM_011543963.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54024	XM_005272659.4	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61260	XM_011543969.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61263	XM_011543972.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54022	XM_005272656.4	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu99448	XM_017029783.1	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu61255	XM_011543964.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu61261	XM_011543970.2	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KDM6A ( NM_021140.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066963.2 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 3, mRNA.

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CloneID	OHu24583
Clone ID Related Accession (Same CDS sequence)	NM_021140.3
Accession Version	NM_021140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, AB208795.1, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced NM_021140.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF000993.1, AB208795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT AAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCTCATTAC TGTAGCATTT GTGAAGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCA CGAAAGACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGCGG AAACTTGGAA AACTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066963.2
CDS	378..4583
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_021140.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021140.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT AAGAACTCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTC ATTGCTGCAG GAAAAGAGAT TATATGGCAT 
GGGCGGACAA AAGAAGAACC AGCTCATTAC TGTAGCATTT GTGAAGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCA CGAAAGACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGCGG AAACTTGGAA AACTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGACCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278344.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.

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CloneID	OHu24601
Clone ID Related Accession (Same CDS sequence)	NM_001291415.2 , NM_001291415.1
Accession Version	NM_001291415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133545.10, AL138744.41 and AC136488.3. On Oct 1, 2019 this sequence version replaced NM_001291415.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143277.1, SRR1660805.126238.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278344.1
CDS	365..4726
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291415.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_024452438.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308206.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu106278
Clone ID Related Accession (Same CDS sequence)	XM_024452438.1
Accession Version	XM_024452438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGATATTTT CATTGTCTCC GAATTTTAGA 
GCTGAAAAGT GCCTTAGAGA TCATCTAGTT CAACCTCTCC GTTCAAATGG AGAACCTGAG 
CCACTAAGAT TCACAGGAGA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG 
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA 
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT 
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC 
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC 
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308206.1
CDS	371..4738
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_024452438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452438.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGATATTTT CATTGTCTCC GAATTTTAGA 
GCTGAAAAGT GCCTTAGAGA TCATCTAGTT CAACCTCTCC GTTCAAATGG AGAACCTGAG 
CCACTAAGAT TCACAGGAGA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG 
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA 
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT 
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC 
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC 
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_024452439.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308207.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu106279
Clone ID Related Accession (Same CDS sequence)	XM_024452439.1
Accession Version	XM_024452439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAAG TGAACACAGA CTATGAGTCT AGTTTAAAGC ATTTTCAGTT AGCTTTGGTT 
GACTGTAATC CCTGCACTTT GTCCAATGCT GAAATTCAAT TTCACATTGC CCACTTATAT 
GAAACCCAGA GGAAATATCA TTCTGCAAAA GAAGCTTATG AACAACTTTT GCAGACAGAG 
AATCTTTCTG CACAAGTAAA AGCAACTGTC TTACAACAGT TAGGTTGGAT GCATCACACT 
GTAGATCTCC TGGGAGATAA AGCCACCAAG GAAAGCTATG CTATTCAGTA TCTCCAAAAG 
TCCTTGGAAG CAGATCCTAA TTCTGGCCAG TCCTGGTATT TCCTCGGAAG GTGCTATTCA 
AGTATTGGGA AAGTTCAGGA TGCCTTTATA TCTTACAGGC AGTCTATTGA TAAATCAGAA 
GCAAGTGCAG ATACATGGTG TTCAATAGGT GTGCTATATC AGCAGCAAAA TCAGCCCATG 
GATGCTTTAC AGGCCTATAT TTGTGCTGTA CAATTGGACC ATGGCCATGC TGCAGCCTGG 
ATGGACCTAG GCACTCTCTA TGAATCCTGC AACCAGCCTC AGGATGCCAT TAAATGCTAC 
TTAAATGCAA CTAGAAGCAA AAGTTGTAGT AATACCTCTG CACTTGCAGC ACGAATTAAG 
TATTTACAGG CTCAGTTGTG TAACCTTCCA CAAGGTAGTC TACAGAATAA AACTAAATTA 
CTTCCTAGTA TTGAGGAGGC GTGGAGCCTA CCAATTCCCG CAGAGCTTAC CTCCAGGCAG 
GGTGCCATGA ACACAGCACA GCAGGCATGT AAACCTCATC ATCCAAATAC TGAACCTGTA 
TTAGGCCTCA GTCAAACACC AATTTCACAG CAATCCTTGC CACTACACAT GATTCCTTCT 
AGCCAAGTAG ATGACCTGTC CAGTCCTGCC AAGAGGAAAA GAACATCTAG TCCAACAAAG 
AATACTTCTG ACAATTGGAG TGGTGGACAT GCTGTGTCAC ATCCTCCAGT ACAGCAACAA 
GCTCATTCAT GGTGTTTGAC ACCACAGAAA TTACAGCATT TGGAACAGCT CCGCGCAAAT 
AGAAATAATT TAAATCCAGC ACAGAAACTG ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC 
TTAATGCAAC AACACCAAAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT 
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA 
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT 
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA 
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT 
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC 
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT 
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT 
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC 
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT 
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC 
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT 
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC 
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG 
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC 
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT 
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC 
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG 
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA 
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA 
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA 
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT 
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG 
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT 
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG 
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT 
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC 
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT 
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG 
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG 
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT 
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA 
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC 
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA 
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA 
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC 
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA 
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT 
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC 
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT 
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA 
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308207.1
CDS	534..4478
Translation	MFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_024452439.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452439.1

ORF Insert Sequence:

ATGTTCAAAG TGAACACAGA CTATGAGTCT AGTTTAAAGC ATTTTCAGTT AGCTTTGGTT 
GACTGTAATC CCTGCACTTT GTCCAATGCT GAAATTCAAT TTCACATTGC CCACTTATAT 
GAAACCCAGA GGAAATATCA TTCTGCAAAA GAAGCTTATG AACAACTTTT GCAGACAGAG 
AATCTTTCTG CACAAGTAAA AGCAACTGTC TTACAACAGT TAGGTTGGAT GCATCACACT 
GTAGATCTCC TGGGAGATAA AGCCACCAAG GAAAGCTATG CTATTCAGTA TCTCCAAAAG 
TCCTTGGAAG CAGATCCTAA TTCTGGCCAG TCCTGGTATT TCCTCGGAAG GTGCTATTCA 
AGTATTGGGA AAGTTCAGGA TGCCTTTATA TCTTACAGGC AGTCTATTGA TAAATCAGAA 
GCAAGTGCAG ATACATGGTG TTCAATAGGT GTGCTATATC AGCAGCAAAA TCAGCCCATG 
GATGCTTTAC AGGCCTATAT TTGTGCTGTA CAATTGGACC ATGGCCATGC TGCAGCCTGG 
ATGGACCTAG GCACTCTCTA TGAATCCTGC AACCAGCCTC AGGATGCCAT TAAATGCTAC 
TTAAATGCAA CTAGAAGCAA AAGTTGTAGT AATACCTCTG CACTTGCAGC ACGAATTAAG 
TATTTACAGG CTCAGTTGTG TAACCTTCCA CAAGGTAGTC TACAGAATAA AACTAAATTA 
CTTCCTAGTA TTGAGGAGGC GTGGAGCCTA CCAATTCCCG CAGAGCTTAC CTCCAGGCAG 
GGTGCCATGA ACACAGCACA GCAGGCATGT AAACCTCATC ATCCAAATAC TGAACCTGTA 
TTAGGCCTCA GTCAAACACC AATTTCACAG CAATCCTTGC CACTACACAT GATTCCTTCT 
AGCCAAGTAG ATGACCTGTC CAGTCCTGCC AAGAGGAAAA GAACATCTAG TCCAACAAAG 
AATACTTCTG ACAATTGGAG TGGTGGACAT GCTGTGTCAC ATCCTCCAGT ACAGCAACAA 
GCTCATTCAT GGTGTTTGAC ACCACAGAAA TTACAGCATT TGGAACAGCT CCGCGCAAAT 
AGAAATAATT TAAATCCAGC ACAGAAACTG ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC 
TTAATGCAAC AACACCAAAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT 
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA 
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT 
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA 
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT 
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC 
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT 
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT 
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC 
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT 
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC 
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT 
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC 
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG 
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC 
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT 
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC 
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG 
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA 
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA 
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA 
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT 
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG 
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT 
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG 
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT 
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC 
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT 
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG 
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG 
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT 
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA 
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC 
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA 
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA 
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC 
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA 
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT 
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC 
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT 
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA 
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_017029782.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885271.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.

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CloneID	OHu99447
Clone ID Related Accession (Same CDS sequence)	XM_017029782.1
Accession Version	XM_017029782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG 
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885271.1
CDS	378..4661
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_017029782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029782.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG 
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_017029783.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885272.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.

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CloneID	OHu99448
Clone ID Related Accession (Same CDS sequence)	XM_017029783.2 , XM_017029783.1
Accession Version	XM_017029783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885272.1
CDS	371..4495
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_017029783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029783.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_017029783.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885272.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu99448
Clone ID Related Accession (Same CDS sequence)	XM_017029783.2 , XM_017029783.1
Accession Version	XM_017029783.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885272.1
CDS	378..4502
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_017029783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029783.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_017029784.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885273.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.

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CloneID	OHu99449
Clone ID Related Accession (Same CDS sequence)	XM_017029784.1
Accession Version	XM_017029784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GGCATGTAAA CCTCATCATC CAAATACTGA ACCTGTATTA 
GGCCTCAGTC AAACACCAAT TTCACAGCAA TCCTTGCCAC TACACATGAT TCCTTCTAGC 
CAAGTAGATG ACCTGTCCAG TCCTGCCAAG AGGAAAAGAA CATCTAGTCC AACAAAGAAT 
ACTTCTGACA ATTGGAGTGG TGGACATGCT GTGTCACATC CTCCAGTACA GCAACAAGCT 
CATTCATGGT GTTTGACACC ACAGAAATTA CAGCATTTGG AACAGCTCCG CGCAAATAGA 
AATAATTTAA ATCCAGCACA GAAACTGATG CTGGAACAGC TGGAAAGTCA GTTTGTCTTA 
ATGCAACAAC ACCAAATGAG ACCAACAGGA GTTGCACAGG TACGATCTAC TGGAATTCCT 
AATGGGCCAA CAGCTGACTC ATCACTGCCT ACAAACTCAG TCTCTGGCCA GCAGCCACAG 
CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG 
CAGCCTTTGG CCAATGGACC CTTTTCTGCA GGCCATGTTC CCTGTAGCAC ATCAAGAACG 
CTGGGAAGTA CAGACACTAT TTTGATAGGC AATAATCATA TAACAGGAAG TGGAAGTAAT 
GGAAACGTGC CTTACCTGCA GCGAAACGCA CTCACTCTAC CTCATAACCG CACAAACCTG 
ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC CAACTATCTA ACTCCACTCA GGGGCTTCAC 
AAAGGTCAGA GTTCACATTC GGCAGGTCCT AATGGTGAAC GACCTCTCTC TTCCACTGGG 
CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT GGTATTCAGA ATCAGAACGG ACATCCCACC 
CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT GCTCTCAATC ACCTCTCCTC TCACACTGCT 
ACCTCAGGTG GACAACAAGG CATTACCTTA ACCAAAGAGA GCAAGCCTTC AGGAAACATA 
TTGACGGTGC CTGAAACAAG CAGGCACACT GGAGAGACAC CTAACAGCAC TGCCAGTGTC 
GAGGGACTTC CTAATCATGT CCATCAGATG ACGGCAGATG CTGTTTGCAG TCCTAGCCAT 
GGAGATTCTA AGTCACCAGG TTTACTAAGT TCAGACAATC CTCAGCTCTC TGCCTTGTTG 
ATGGGAAAAG CCAATAACAA TGTGGGTACT GGAACCTGTG ACAAAGTCAA TAACATCCAC 
CCAGCTGTTC ATACAAAGAC TGATAACTCT GTTGCCTCTT CACCATCTTC AGCCATTTCA 
ACAGCAACAC CTTCTCCAAA ATCCACTGAG CAGACAACCA CAAACAGTGT TACCAGCCTT 
AACAGCCCTC ACAGTGGGCT ACACACAATT AATGGAGAAG GGATGGAAGA ATCTCAGAGC 
CCCATGAAAA CAGATCTGCT TCTGGTTAAC CACAAACCTA GTCCACAGAT CATACCATCA 
ATGTCTGTGT CCATATACCC CAGCTCAGCA GAAGTTCTGA AGGCATGCAG GAATCTAGGT 
AAAAATGGCT TATCTAACAG TAGCATTTTG TTGGATAAAT GTCCACCTCC AAGACCACCA 
TCTTCACCAT ACCCTCCCTT GCCAAAGGAC AAGTTGAATC CACCTACACC TAGTATTTAC 
TTGGAAAATA AACGTGATGC TTTCTTTCCT CCATTACATC AATTTTGTAC AAATCCGAAC 
AACCCTGTTA CAGTAATACG TGGCCTTGCT GGAGCTCTTA AGTTAGACCT GGGACTTTTC 
TCTACTAAAA CTTTGGTGGA AGCTAACAAT GAACATATGG TAGAAGTGAG GACACAGTTG 
TTGCAGCCAG CAGATGAAAA CTGGGATCCC ACTGGAACAA AGAAAATCTG GCATTGTGAA 
AGTAATAGAT CTCATACTAC AATTGCTAAA TATGCACAGT ACCAGGCCTC CTCATTCCAG 
GAATCATTGA GAGAAGAAAA TGAAAAAAGA AGTCATCATA AAGACCACTC AGATAGTGAA 
TCTACATCGT CAGATAATTC TGGGAGGAGG AGGAAAGGAC CCTTTAAAAC CATAAAGTTT 
GGGACCAATA TTGACCTATC TGATGACAAA AAGTGGAAGT TGCAGCTACA TGAGCTGACT 
AAACTTCCTG CTTTTGTGCG TGTCGTATCA GCAGGAAATC TTCTAAGCCA TGTTGGTCAT 
ACCATATTGG GCATGAACAC AGTTCAACTA TACATGAAAG TTCCAGGGAG CAGAACACCA 
GGTCATCAGG AAAATAACAA CTTCTGTTCA GTTAACATAA ATATTGGCCC AGGTGACTGT 
GAATGGTTTG TTGTTCCTGA AGGTTACTGG GGTGTTCTGA ATGACTTCTG TGAAAAAAAT 
AATTTGAATT TCCTAATGGG TTCTTGGTGG CCCAATCTTG AAGATCTTTA TGAAGCAAAT 
GTTCCAGTGT ATAGGTTTAT TCAGCGACCT GGAGATTTGG TCTGGATAAA TGCAGGCACT 
GTTCATTGGG TTCAGGCTAT TGGCTGGTGC AACAACATTG CTTGGAATGT TGGTCCACTT 
ACAGCCTGCC AGTATAAATT GGCAGTGGAA CGGTACGAAT GGAACAAATT GCAAAGTGTG 
AAGTCAATAG TACCCATGGT TCATCTTTCC TGGAATATGG CACGAAATAT CAAGGTCTCA 
GATCCAAAGC TTTTTGAAAT GATTAAGATA TTTTCATTGT CTCCGAATTT TAGAGCTGAA 
AAGTGCCTTA GAGATCATCT AGTTCAACCT CTCCGTTCAA ATGGAGAACC TGAGCCACTA 
AGATTCACAG GAGAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885273.1
CDS	576..4157
Translation	MHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_017029784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029784.1

ORF Insert Sequence:

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GGCATGTAAA CCTCATCATC CAAATACTGA ACCTGTATTA 
GGCCTCAGTC AAACACCAAT TTCACAGCAA TCCTTGCCAC TACACATGAT TCCTTCTAGC 
CAAGTAGATG ACCTGTCCAG TCCTGCCAAG AGGAAAAGAA CATCTAGTCC AACAAAGAAT 
ACTTCTGACA ATTGGAGTGG TGGACATGCT GTGTCACATC CTCCAGTACA GCAACAAGCT 
CATTCATGGT GTTTGACACC ACAGAAATTA CAGCATTTGG AACAGCTCCG CGCAAATAGA 
AATAATTTAA ATCCAGCACA GAAACTGATG CTGGAACAGC TGGAAAGTCA GTTTGTCTTA 
ATGCAACAAC ACCAAATGAG ACCAACAGGA GTTGCACAGG TACGATCTAC TGGAATTCCT 
AATGGGCCAA CAGCTGACTC ATCACTGCCT ACAAACTCAG TCTCTGGCCA GCAGCCACAG 
CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG 
CAGCCTTTGG CCAATGGACC CTTTTCTGCA GGCCATGTTC CCTGTAGCAC ATCAAGAACG 
CTGGGAAGTA CAGACACTAT TTTGATAGGC AATAATCATA TAACAGGAAG TGGAAGTAAT 
GGAAACGTGC CTTACCTGCA GCGAAACGCA CTCACTCTAC CTCATAACCG CACAAACCTG 
ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC CAACTATCTA ACTCCACTCA GGGGCTTCAC 
AAAGGTCAGA GTTCACATTC GGCAGGTCCT AATGGTGAAC GACCTCTCTC TTCCACTGGG 
CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT GGTATTCAGA ATCAGAACGG ACATCCCACC 
CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT GCTCTCAATC ACCTCTCCTC TCACACTGCT 
ACCTCAGGTG GACAACAAGG CATTACCTTA ACCAAAGAGA GCAAGCCTTC AGGAAACATA 
TTGACGGTGC CTGAAACAAG CAGGCACACT GGAGAGACAC CTAACAGCAC TGCCAGTGTC 
GAGGGACTTC CTAATCATGT CCATCAGATG ACGGCAGATG CTGTTTGCAG TCCTAGCCAT 
GGAGATTCTA AGTCACCAGG TTTACTAAGT TCAGACAATC CTCAGCTCTC TGCCTTGTTG 
ATGGGAAAAG CCAATAACAA TGTGGGTACT GGAACCTGTG ACAAAGTCAA TAACATCCAC 
CCAGCTGTTC ATACAAAGAC TGATAACTCT GTTGCCTCTT CACCATCTTC AGCCATTTCA 
ACAGCAACAC CTTCTCCAAA ATCCACTGAG CAGACAACCA CAAACAGTGT TACCAGCCTT 
AACAGCCCTC ACAGTGGGCT ACACACAATT AATGGAGAAG GGATGGAAGA ATCTCAGAGC 
CCCATGAAAA CAGATCTGCT TCTGGTTAAC CACAAACCTA GTCCACAGAT CATACCATCA 
ATGTCTGTGT CCATATACCC CAGCTCAGCA GAAGTTCTGA AGGCATGCAG GAATCTAGGT 
AAAAATGGCT TATCTAACAG TAGCATTTTG TTGGATAAAT GTCCACCTCC AAGACCACCA 
TCTTCACCAT ACCCTCCCTT GCCAAAGGAC AAGTTGAATC CACCTACACC TAGTATTTAC 
TTGGAAAATA AACGTGATGC TTTCTTTCCT CCATTACATC AATTTTGTAC AAATCCGAAC 
AACCCTGTTA CAGTAATACG TGGCCTTGCT GGAGCTCTTA AGTTAGACCT GGGACTTTTC 
TCTACTAAAA CTTTGGTGGA AGCTAACAAT GAACATATGG TAGAAGTGAG GACACAGTTG 
TTGCAGCCAG CAGATGAAAA CTGGGATCCC ACTGGAACAA AGAAAATCTG GCATTGTGAA 
AGTAATAGAT CTCATACTAC AATTGCTAAA TATGCACAGT ACCAGGCCTC CTCATTCCAG 
GAATCATTGA GAGAAGAAAA TGAAAAAAGA AGTCATCATA AAGACCACTC AGATAGTGAA 
TCTACATCGT CAGATAATTC TGGGAGGAGG AGGAAAGGAC CCTTTAAAAC CATAAAGTTT 
GGGACCAATA TTGACCTATC TGATGACAAA AAGTGGAAGT TGCAGCTACA TGAGCTGACT 
AAACTTCCTG CTTTTGTGCG TGTCGTATCA GCAGGAAATC TTCTAAGCCA TGTTGGTCAT 
ACCATATTGG GCATGAACAC AGTTCAACTA TACATGAAAG TTCCAGGGAG CAGAACACCA 
GGTCATCAGG AAAATAACAA CTTCTGTTCA GTTAACATAA ATATTGGCCC AGGTGACTGT 
GAATGGTTTG TTGTTCCTGA AGGTTACTGG GGTGTTCTGA ATGACTTCTG TGAAAAAAAT 
AATTTGAATT TCCTAATGGG TTCTTGGTGG CCCAATCTTG AAGATCTTTA TGAAGCAAAT 
GTTCCAGTGT ATAGGTTTAT TCAGCGACCT GGAGATTTGG TCTGGATAAA TGCAGGCACT 
GTTCATTGGG TTCAGGCTAT TGGCTGGTGC AACAACATTG CTTGGAATGT TGGTCCACTT 
ACAGCCTGCC AGTATAAATT GGCAGTGGAA CGGTACGAAT GGAACAAATT GCAAAGTGTG 
AAGTCAATAG TACCCATGGT TCATCTTTCC TGGAATATGG CACGAAATAT CAAGGTCTCA 
GATCCAAAGC TTTTTGAAAT GATTAAGATA TTTTCATTGT CTCCGAATTT TAGAGCTGAA 
AAGTGCCTTA GAGATCATCT AGTTCAACCT CTCCGTTCAA ATGGAGAACC TGAGCCACTA 
AGATTCACAG GAGAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_017029785.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885274.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.

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CloneID	OHu99450
Clone ID Related Accession (Same CDS sequence)	XM_017029785.1
Accession Version	XM_017029785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA 
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGATG 
AGACCAACAG GAGTTGCACA GGTACGATCT ACTGGAATTC CTAATGGGCC AACAGCTGAC 
TCATCACTGC CTACAAACTC AGTCTCTGGC CAGCAGCCAC AGCTTGCTCT GACCAGAGTG 
CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT GCCTGCCCTG GGCAGCCTTT GGCCAATGGA 
CCCTTTTCTG CAGGCCATGT TCCCTGTAGC ACATCAAGAA CGCTGGGAAG TACAGACACT 
ATTTTGATAG GCAATAATCA TATAACAGGA AGTGGAAGTA ATGGAAACGT GCCTTACCTG 
CAGCGAAACG CACTCACTCT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTATC TAACTCCACT CAGGGGCTTC ACAAAGGTCA GAGTTCACAT 
TCGGCAGGTC CTAATGGTGA ACGACCTCTC TCTTCCACTG GGCCTTCCCA GCATCTCCAG 
GCAGCTGGCT CTGGTATTCA GAATCAGAAC GGACATCCCA CCCTGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTACCT TAACCAAAGA GAGCAAGCCT TCAGGAAACA TATTGACGGT GCCTGAAACA 
AGCAGGCACA CTGGAGAGAC ACCTAACAGC ACTGCCAGTG TCGAGGGACT TCCTAATCAT 
GTCCATCAGA TGACGGCAGA TGCTGTTTGC AGTCCTAGCC ATGGAGATTC TAAGTCACCA 
GGTTTACTAA GTTCAGACAA TCCTCAGCTC TCTGCCTTGT TGATGGGAAA AGCCAATAAC 
AATGTGGGTA CTGGAACCTG TGACAAAGTC AATAACATCC ACCCAGCTGT TCATACAAAG 
ACTGATAACT CTGTTGCCTC TTCACCATCT TCAGCCATTT CAACAGCAAC ACCTTCTCCA 
AAATCCACTG AGCAGACAAC CACAAACAGT GTTACCAGCC TTAACAGCCC TCACAGTGGG 
CTACACACAA TTAATGGAGA AGGGATGGAA GAATCTCAGA GCCCCATGAA AACAGATCTG 
CTTCTGGTTA ACCACAAACC TAGTCCACAG ATCATACCAT CAATGTCTGT GTCCATATAC 
CCCAGCTCAG CAGAAGTTCT GAAGGCATGC AGGAATCTAG GTAAAAATGG CTTATCTAAC 
AGTAGCATTT TGTTGGATAA ATGTCCACCT CCAAGACCAC CATCTTCACC ATACCCTCCC 
TTGCCAAAGG ACAAGTTGAA TCCACCTACA CCTAGTATTT ACTTGGAAAA TAAACGTGAT 
GCTTTCTTTC CTCCATTACA TCAATTTTGT ACAAATCCGA ACAACCCTGT TACAGTAATA 
CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC CTGGGACTTT TCTCTACTAA AACTTTGGTG 
GAAGCTAACA ATGAACATAT GGTAGAAGTG AGGACACAGT TGTTGCAGCC AGCAGATGAA 
AACTGGGATC CCACTGGAAC AAAGAAAATC TGGCATTGTG AAAGTAATAG ATCTCATACT 
ACAATTGCTA AATATGCACA GTACCAGGCC TCCTCATTCC AGGAATCATT GAGAGAAGAA 
AATGAAAAAA GAAGTCATCA TAAAGACCAC TCAGATAGTG AATCTACATC GTCAGATAAT 
TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA ACCATAAAGT TTGGGACCAA TATTGACCTA 
TCTGATGACA AAAAGTGGAA GTTGCAGCTA CATGAGCTGA CTAAACTTCC TGCTTTTGTG 
CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC CATGTTGGTC ATACCATATT GGGCATGAAC 
ACAGTTCAAC TATACATGAA AGTTCCAGGG AGCAGAACAC CAGGTCATCA GGAAAATAAC 
AACTTCTGTT CAGTTAACAT AAATATTGGC CCAGGTGACT GTGAATGGTT TGTTGTTCCT 
GAAGGTTACT GGGGTGTTCT GAATGACTTC TGTGAAAAAA ATAATTTGAA TTTCCTAATG 
GGTTCTTGGT GGCCCAATCT TGAAGATCTT TATGAAGCAA ATGTTCCAGT GTATAGGTTT 
ATTCAGCGAC CTGGAGATTT GGTCTGGATA AATGCAGGCA CTGTTCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCTTGGAAT GTTGGTCCAC TTACAGCCTG CCAGTATAAA 
TTGGCAGTGG AACGGTACGA ATGGAACAAA TTGCAAAGTG TGAAGTCAAT AGTACCCATG 
GTTCATCTTT CCTGGAATAT GGCACGAAAT ATCAAGGTCT CAGATCCAAA GCTTTTTGAA 
ATGATTAAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC 
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC 
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA 
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA 
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT 
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885274.1
CDS	807..4022
Translation	MHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_017029785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029785.1

ORF Insert Sequence:

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA 
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGATG 
AGACCAACAG GAGTTGCACA GGTACGATCT ACTGGAATTC CTAATGGGCC AACAGCTGAC 
TCATCACTGC CTACAAACTC AGTCTCTGGC CAGCAGCCAC AGCTTGCTCT GACCAGAGTG 
CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT GCCTGCCCTG GGCAGCCTTT GGCCAATGGA 
CCCTTTTCTG CAGGCCATGT TCCCTGTAGC ACATCAAGAA CGCTGGGAAG TACAGACACT 
ATTTTGATAG GCAATAATCA TATAACAGGA AGTGGAAGTA ATGGAAACGT GCCTTACCTG 
CAGCGAAACG CACTCACTCT ACCTCATAAC CGCACAAACC TGACCAGCAG CGCAGAGGAG 
CCGTGGAAAA ACCAACTATC TAACTCCACT CAGGGGCTTC ACAAAGGTCA GAGTTCACAT 
TCGGCAGGTC CTAATGGTGA ACGACCTCTC TCTTCCACTG GGCCTTCCCA GCATCTCCAG 
GCAGCTGGCT CTGGTATTCA GAATCAGAAC GGACATCCCA CCCTGCCTAG CAATTCAGTA 
ACACAGGGGG CTGCTCTCAA TCACCTCTCC TCTCACACTG CTACCTCAGG TGGACAACAA 
GGCATTACCT TAACCAAAGA GAGCAAGCCT TCAGGAAACA TATTGACGGT GCCTGAAACA 
AGCAGGCACA CTGGAGAGAC ACCTAACAGC ACTGCCAGTG TCGAGGGACT TCCTAATCAT 
GTCCATCAGA TGACGGCAGA TGCTGTTTGC AGTCCTAGCC ATGGAGATTC TAAGTCACCA 
GGTTTACTAA GTTCAGACAA TCCTCAGCTC TCTGCCTTGT TGATGGGAAA AGCCAATAAC 
AATGTGGGTA CTGGAACCTG TGACAAAGTC AATAACATCC ACCCAGCTGT TCATACAAAG 
ACTGATAACT CTGTTGCCTC TTCACCATCT TCAGCCATTT CAACAGCAAC ACCTTCTCCA 
AAATCCACTG AGCAGACAAC CACAAACAGT GTTACCAGCC TTAACAGCCC TCACAGTGGG 
CTACACACAA TTAATGGAGA AGGGATGGAA GAATCTCAGA GCCCCATGAA AACAGATCTG 
CTTCTGGTTA ACCACAAACC TAGTCCACAG ATCATACCAT CAATGTCTGT GTCCATATAC 
CCCAGCTCAG CAGAAGTTCT GAAGGCATGC AGGAATCTAG GTAAAAATGG CTTATCTAAC 
AGTAGCATTT TGTTGGATAA ATGTCCACCT CCAAGACCAC CATCTTCACC ATACCCTCCC 
TTGCCAAAGG ACAAGTTGAA TCCACCTACA CCTAGTATTT ACTTGGAAAA TAAACGTGAT 
GCTTTCTTTC CTCCATTACA TCAATTTTGT ACAAATCCGA ACAACCCTGT TACAGTAATA 
CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC CTGGGACTTT TCTCTACTAA AACTTTGGTG 
GAAGCTAACA ATGAACATAT GGTAGAAGTG AGGACACAGT TGTTGCAGCC AGCAGATGAA 
AACTGGGATC CCACTGGAAC AAAGAAAATC TGGCATTGTG AAAGTAATAG ATCTCATACT 
ACAATTGCTA AATATGCACA GTACCAGGCC TCCTCATTCC AGGAATCATT GAGAGAAGAA 
AATGAAAAAA GAAGTCATCA TAAAGACCAC TCAGATAGTG AATCTACATC GTCAGATAAT 
TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA ACCATAAAGT TTGGGACCAA TATTGACCTA 
TCTGATGACA AAAAGTGGAA GTTGCAGCTA CATGAGCTGA CTAAACTTCC TGCTTTTGTG 
CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC CATGTTGGTC ATACCATATT GGGCATGAAC 
ACAGTTCAAC TATACATGAA AGTTCCAGGG AGCAGAACAC CAGGTCATCA GGAAAATAAC 
AACTTCTGTT CAGTTAACAT AAATATTGGC CCAGGTGACT GTGAATGGTT TGTTGTTCCT 
GAAGGTTACT GGGGTGTTCT GAATGACTTC TGTGAAAAAA ATAATTTGAA TTTCCTAATG 
GGTTCTTGGT GGCCCAATCT TGAAGATCTT TATGAAGCAA ATGTTCCAGT GTATAGGTTT 
ATTCAGCGAC CTGGAGATTT GGTCTGGATA AATGCAGGCA CTGTTCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCTTGGAAT GTTGGTCCAC TTACAGCCTG CCAGTATAAA 
TTGGCAGTGG AACGGTACGA ATGGAACAAA TTGCAAAGTG TGAAGTCAAT AGTACCCATG 
GTTCATCTTT CCTGGAATAT GGCACGAAAT ATCAAGGTCT CAGATCCAAA GCTTTTTGAA 
ATGATTAAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC 
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC 
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA 
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA 
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT 
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291421.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278350.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu24600
Clone ID Related Accession (Same CDS sequence)	NM_001291421.1
Accession Version	NM_001291421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143275.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA 
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGCAT 
TTGGAACAGC TCCGCGCAAA TAGAAATAAT TTAAATCCAG CACAGAAACT GATGCTGGAA 
CAGCTGGAAA GTCAGTTTGT CTTAATGCAA CAACACCAAA TGAGACCAAC AGGAGTTGCA 
CAGGTACGAT CTACTGGAAT TCCTAATGGG CCAACAGCTG ACTCATCACT GCCTACAAAC 
TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT CTGACCAGAG TGCCTAGCGT CTCTCAGCCT 
GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT TTGGCCAATG GACCCTTTTC TGCAGGCCAT 
GTTCCCTGTA GCACATCAAG AACGCTGGGA AGTACAGACA CTATTTTGAT AGGCAATAAT 
CATATAACAG GAAGTGGAAG TAATGGAAAC GTGCCTTACC TGCAGCGAAA CGCACTCACT 
CTACCTCATA ACCGCACAAA CCTGACCAGC AGCGCAGAGG AGCCGTGGAA AAACCAACTA 
TCTAACTCCA CTCAGGGGCT TCACAAAGGT CAGAGTTCAC ATTCGGCAGG TCCTAATGGT 
GAACGACCTC TCTCTTCCAC TGGGCCTTCC CAGCATCTCC AGGCAGCTGG CTCTGGTATT 
CAGAATCAGA ACGGACATCC CACCCTGCCT AGCAATTCAG TAACACAGGG GGCTGCTCTC 
AATCACCTCT CCTCTCACAC TGCTACCTCA GGTGGACAAC AAGGCATTAC CTTAACCAAA 
GAGAGCAAGC CTTCAGGAAA CATATTGACG GTGCCTGAAA CAAGCAGGCA CACTGGAGAG 
ACACCTAACA GCACTGCCAG TGTCGAGGGA CTTCCTAATC ATGTCCATCA GATGACGGCA 
GATGCTGTTT GCAGTCCTAG CCATGGAGAT TCTAAGTCAC CAGGTTTACT AAGTTCAGAC 
AATCCTCAGC TCTCTGCCTT GTTGATGGGA AAAGCCAATA ACAATGTGGG TACTGGAACC 
TGTGACAAAG TCAATAACAT CCACCCAGCT GTTCATACAA AGACTGATAA CTCTGTTGCC 
TCTTCACCAT CTTCAGCCAT TTCAACAGCA ACACCTTCTC CAAAATCCAC TGAGCAGACA 
ACCACAAACA GTGTTACCAG CCTTAACAGC CCTCACAGTG GGCTACACAC AATTAATGGA 
GAAGGGATGG AAGAATCTCA GAGCCCCATG AAAACAGATC TGCTTCTGGT TAACCACAAA 
CCTAGTCCAC AGATCATACC ATCAATGTCT GTGTCCATAT ACCCCAGCTC AGCAGAAGTT 
CTGAAGGCAT GCAGGAATCT AGGTAAAAAT GGCTTATCTA ACAGTAGCAT TTTGTTGGAT 
AAATGTCCAC CTCCAAGACC ACCATCTTCA CCATACCCTC CCTTGCCAAA GGACAAGTTG 
AATCCACCTA CACCTAGTAT TTACTTGGAA AATAAACGTG ATGCTTTCTT TCCTCCATTA 
CATCAATTTT GTACAAATCC GAACAACCCT GTTACAGTAA TACGTGGCCT TGCTGGAGCT 
CTTAAGTTAG ACCTGGGACT TTTCTCTACT AAAACTTTGG TGGAAGCTAA CAATGAACAT 
ATGGTAGAAG TGAGGACACA GTTGTTGCAG CCAGCAGATG AAAACTGGGA TCCCACTGGA 
ACAAAGAAAA TCTGGCATTG TGAAAGTAAT AGATCTCATA CTACAATTGC TAAATATGCA 
CAGTACCAGG CCTCCTCATT CCAGGAATCA TTGAGAGAAG AAAATGAAAA AAGAAGTCAT 
CATAAAGACC ACTCAGATAG TGAATCTACA TCGTCAGATA ATTCTGGGAG GAGGAGGAAA 
GGACCCTTTA AAACCATAAA GTTTGGGACC AATATTGACC TATCTGATGA CAAAAAGTGG 
AAGTTGCAGC TACATGAGCT GACTAAACTT CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA 
AATCTTCTAA GCCATGTTGG TCATACCATA TTGGGCATGA ACACAGTTCA ACTATACATG 
AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT CAGGAAAATA ACAACTTCTG TTCAGTTAAC 
ATAAATATTG GCCCAGGTGA CTGTGAATGG TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT 
CTGAATGACT TCTGTGAAAA AAATAATTTG AATTTCCTAA TGGGTTCTTG GTGGCCCAAT 
CTTGAAGATC TTTATGAAGC AAATGTTCCA GTGTATAGGT TTATTCAGCG ACCTGGAGAT 
TTGGTCTGGA TAAATGCAGG CACTGTTCAT TGGGTTCAGG CTATTGGCTG GTGCAACAAC 
ATTGCTTGGA ATGTTGGTCC ACTTACAGCC TGCCAGTATA AATTGGCAGT GGAACGGTAC 
GAATGGAACA AATTGCAAAG TGTGAAGTCA ATAGTACCCA TGGTTCATCT TTCCTGGAAT 
ATGGCACGAA ATATCAAGGT CTCAGATCCA AAGCTTTTTG AAATGATTAA GTATTGTCTT 
CTAAGAACTC TGAAGCAATG TCAGACATTG AGGGAAGCTC TCATTGCTGC AGGAAAAGAG 
ATTATATGGC ATGGGCGGAC AAAAGAAGAA CCAGCTCATT ACTGTAGCAT TTGTGAAGTG 
GAGGTTTTTG ATCTGCTTTT TGTCACTAAT GAGAGTAATT CACGAAAGAC CTACATAGTA 
CATTGCCAAG ATTGTGCACG AAAAACAAGC GGAAACTTGG AAAACTTTGT GGTGCTAGAA 
CAGTACAAAA TGGAGGACCT GATGCAAGTC TATGACCAAT TTACATTAGC TCCTCCATTA 
CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278350.1
CDS	1010..4327
Translation	MHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291421.1

ORF Insert Sequence:

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GAATACTTCT GACAATTGGA GTGGTGGACA TGCTGTGTCA 
CATCCTCCAG TACAGCAACA AGCTCATTCA TGGTGTTTGA CACCACAGAA ATTACAGCAT 
TTGGAACAGC TCCGCGCAAA TAGAAATAAT TTAAATCCAG CACAGAAACT GATGCTGGAA 
CAGCTGGAAA GTCAGTTTGT CTTAATGCAA CAACACCAAA TGAGACCAAC AGGAGTTGCA 
CAGGTACGAT CTACTGGAAT TCCTAATGGG CCAACAGCTG ACTCATCACT GCCTACAAAC 
TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT CTGACCAGAG TGCCTAGCGT CTCTCAGCCT 
GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT TTGGCCAATG GACCCTTTTC TGCAGGCCAT 
GTTCCCTGTA GCACATCAAG AACGCTGGGA AGTACAGACA CTATTTTGAT AGGCAATAAT 
CATATAACAG GAAGTGGAAG TAATGGAAAC GTGCCTTACC TGCAGCGAAA CGCACTCACT 
CTACCTCATA ACCGCACAAA CCTGACCAGC AGCGCAGAGG AGCCGTGGAA AAACCAACTA 
TCTAACTCCA CTCAGGGGCT TCACAAAGGT CAGAGTTCAC ATTCGGCAGG TCCTAATGGT 
GAACGACCTC TCTCTTCCAC TGGGCCTTCC CAGCATCTCC AGGCAGCTGG CTCTGGTATT 
CAGAATCAGA ACGGACATCC CACCCTGCCT AGCAATTCAG TAACACAGGG GGCTGCTCTC 
AATCACCTCT CCTCTCACAC TGCTACCTCA GGTGGACAAC AAGGCATTAC CTTAACCAAA 
GAGAGCAAGC CTTCAGGAAA CATATTGACG GTGCCTGAAA CAAGCAGGCA CACTGGAGAG 
ACACCTAACA GCACTGCCAG TGTCGAGGGA CTTCCTAATC ATGTCCATCA GATGACGGCA 
GATGCTGTTT GCAGTCCTAG CCATGGAGAT TCTAAGTCAC CAGGTTTACT AAGTTCAGAC 
AATCCTCAGC TCTCTGCCTT GTTGATGGGA AAAGCCAATA ACAATGTGGG TACTGGAACC 
TGTGACAAAG TCAATAACAT CCACCCAGCT GTTCATACAA AGACTGATAA CTCTGTTGCC 
TCTTCACCAT CTTCAGCCAT TTCAACAGCA ACACCTTCTC CAAAATCCAC TGAGCAGACA 
ACCACAAACA GTGTTACCAG CCTTAACAGC CCTCACAGTG GGCTACACAC AATTAATGGA 
GAAGGGATGG AAGAATCTCA GAGCCCCATG AAAACAGATC TGCTTCTGGT TAACCACAAA 
CCTAGTCCAC AGATCATACC ATCAATGTCT GTGTCCATAT ACCCCAGCTC AGCAGAAGTT 
CTGAAGGCAT GCAGGAATCT AGGTAAAAAT GGCTTATCTA ACAGTAGCAT TTTGTTGGAT 
AAATGTCCAC CTCCAAGACC ACCATCTTCA CCATACCCTC CCTTGCCAAA GGACAAGTTG 
AATCCACCTA CACCTAGTAT TTACTTGGAA AATAAACGTG ATGCTTTCTT TCCTCCATTA 
CATCAATTTT GTACAAATCC GAACAACCCT GTTACAGTAA TACGTGGCCT TGCTGGAGCT 
CTTAAGTTAG ACCTGGGACT TTTCTCTACT AAAACTTTGG TGGAAGCTAA CAATGAACAT 
ATGGTAGAAG TGAGGACACA GTTGTTGCAG CCAGCAGATG AAAACTGGGA TCCCACTGGA 
ACAAAGAAAA TCTGGCATTG TGAAAGTAAT AGATCTCATA CTACAATTGC TAAATATGCA 
CAGTACCAGG CCTCCTCATT CCAGGAATCA TTGAGAGAAG AAAATGAAAA AAGAAGTCAT 
CATAAAGACC ACTCAGATAG TGAATCTACA TCGTCAGATA ATTCTGGGAG GAGGAGGAAA 
GGACCCTTTA AAACCATAAA GTTTGGGACC AATATTGACC TATCTGATGA CAAAAAGTGG 
AAGTTGCAGC TACATGAGCT GACTAAACTT CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA 
AATCTTCTAA GCCATGTTGG TCATACCATA TTGGGCATGA ACACAGTTCA ACTATACATG 
AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT CAGGAAAATA ACAACTTCTG TTCAGTTAAC 
ATAAATATTG GCCCAGGTGA CTGTGAATGG TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT 
CTGAATGACT TCTGTGAAAA AAATAATTTG AATTTCCTAA TGGGTTCTTG GTGGCCCAAT 
CTTGAAGATC TTTATGAAGC AAATGTTCCA GTGTATAGGT TTATTCAGCG ACCTGGAGAT 
TTGGTCTGGA TAAATGCAGG CACTGTTCAT TGGGTTCAGG CTATTGGCTG GTGCAACAAC 
ATTGCTTGGA ATGTTGGTCC ACTTACAGCC TGCCAGTATA AATTGGCAGT GGAACGGTAC 
GAATGGAACA AATTGCAAAG TGTGAAGTCA ATAGTACCCA TGGTTCATCT TTCCTGGAAT 
ATGGCACGAA ATATCAAGGT CTCAGATCCA AAGCTTTTTG AAATGATTAA GTATTGTCTT 
CTAAGAACTC TGAAGCAATG TCAGACATTG AGGGAAGCTC TCATTGCTGC AGGAAAAGAG 
ATTATATGGC ATGGGCGGAC AAAAGAAGAA CCAGCTCATT ACTGTAGCAT TTGTGAAGTG 
GAGGTTTTTG ATCTGCTTTT TGTCACTAAT GAGAGTAATT CACGAAAGAC CTACATAGTA 
CATTGCCAAG ATTGTGCACG AAAAACAAGC GGAAACTTGG AAAACTTTGT GGTGCTAGAA 
CAGTACAAAA TGGAGGACCT GATGCAAGTC TATGACCAAT TTACATTAGC TCCTCCATTA 
CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291418.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278347.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 5, mRNA.

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CloneID	OHu24605
Clone ID Related Accession (Same CDS sequence)	NM_001291418.1
Accession Version	NM_001291418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143278.1, DQ062677.1, AB208795.1, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced XM_005272661.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ062677.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACCAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG 
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA 
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT 
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA 
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA 
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC 
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA 
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT 
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTAAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT 
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA 
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278347.1
CDS	378..4346
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291418.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291418.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG ATGAGACCAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG 
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA 
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT 
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA 
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA 
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC 
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA 
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT 
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGTATTGTCT TCTAAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT 
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA 
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543974.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542276.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu61265
Clone ID Related Accession (Same CDS sequence)	XM_011543974.2 , XM_011543974.1
Accession Version	XM_011543974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542276.1
CDS	371..4252
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIK

Target ORF information:

RefSeq Version	XM_011543974.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543974.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543974.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542276.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu61265
Clone ID Related Accession (Same CDS sequence)	XM_011543974.2 , XM_011543974.1
Accession Version	XM_011543974.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542276.1
CDS	378..4259
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIK

Target ORF information:

RefSeq Version	XM_011543974.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543974.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543975.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542277.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.

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CloneID	OHu61266
Clone ID Related Accession (Same CDS sequence)	XM_011543975.2
Accession Version	XM_011543975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCGTGGAGCC TACCAATTCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAATCCTT GCCACTACAC 
ATGATTCCTT CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA 
GTACAGCAAC AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGCA TTTGGAACAG 
CTCCGCGCAA ATAGAAATAA TTTAAATCCA GCACAGAAAC TGATGCTGGA ACAGCTGGAA 
AGTCAGTTTG TCTTAATGCA ACAACACCAA ATGAGACCAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG 
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA 
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT 
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA 
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA 
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC 
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA 
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT 
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGATATTTTC ATTGTCTCCG 
AATTTTAGAG CTGAAAAGTG CCTTAGAGAT CATCTAGTTC AACCTCTCCG TTCAAATGGA 
GAACCTGAGC CACTAAGATT CACAGGAGAG TATTGTCTTC TAAGAACTCT GAAGCAATGT 
CAGACATTGA GGGAAGCTCT CATTGCTGCA GGAAAAGAGA TTATATGGCA TGGGCGGACA 
AAAGAAGAAC CAGCTCATTA CTGTAGCATT TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT 
GTCACTAATG AGAGTAATTC ACGAAAGACC TACATAGTAC ATTGCCAAGA TTGTGCACGA 
AAAACAAGCG GAAACTTGGA AAACTTTGTG GTGCTAGAAC AGTACAAAAT GGAGGACCTG 
ATGCAAGTCT ATGACCAATT TACATTAGCT CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542277.1
CDS	301..4017
Translation	MHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543975.2

ORF Insert Sequence:

ATGCATCACA CTGTAGATCT CCTGGGAGAT AAAGCCACCA AGGAAAGCTA TGCTATTCAG 
TATCTCCAAA AGTCCTTGGA AGCAGATCCT AATTCTGGCC AGTCCTGGTA TTTCCTCGGA 
AGGTGCTATT CAAGTATTGG GAAAGTTCAG GATGCCTTTA TATCTTACAG GCAGTCTATT 
GATAAATCAG AAGCAAGTGC AGATACATGG TGTTCAATAG GTGTGCTATA TCAGCAGCAA 
AATCAGCCCA TGGATGCTTT ACAGGCCTAT ATTTGTGCTG TACAATTGGA CCATGGCCAT 
GCTGCAGCCT GGATGGACCT AGGCACTCTC TATGAATCCT GCAACCAGCC TCAGGATGCC 
ATTAAATGCT ACTTAAATGC AACTAGAAGC AAAAGTTGTA GTAATACCTC TGCACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCGTGGAGCC TACCAATTCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAATCCTT GCCACTACAC 
ATGATTCCTT CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGACAATTGG AGTGGTGGAC ATGCTGTGTC ACATCCTCCA 
GTACAGCAAC AAGCTCATTC ATGGTGTTTG ACACCACAGA AATTACAGCA TTTGGAACAG 
CTCCGCGCAA ATAGAAATAA TTTAAATCCA GCACAGAAAC TGATGCTGGA ACAGCTGGAA 
AGTCAGTTTG TCTTAATGCA ACAACACCAA ATGAGACCAA CAGGAGTTGC ACAGGTACGA 
TCTACTGGAA TTCCTAATGG GCCAACAGCT GACTCATCAC TGCCTACAAA CTCAGTCTCT 
GGCCAGCAGC CACAGCTTGC TCTGACCAGA GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT 
CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT GGACCCTTTT CTGCAGGCCA TGTTCCCTGT 
AGCACATCAA GAACGCTGGG AAGTACAGAC ACTATTTTGA TAGGCAATAA TCATATAACA 
GGAAGTGGAA GTAATGGAAA CGTGCCTTAC CTGCAGCGAA ACGCACTCAC TCTACCTCAT 
AACCGCACAA ACCTGACCAG CAGCGCAGAG GAGCCGTGGA AAAACCAACT ATCTAACTCC 
ACTCAGGGGC TTCACAAAGG TCAGAGTTCA CATTCGGCAG GTCCTAATGG TGAACGACCT 
CTCTCTTCCA CTGGGCCTTC CCAGCATCTC CAGGCAGCTG GCTCTGGTAT TCAGAATCAG 
AACGGACATC CCACCCTGCC TAGCAATTCA GTAACACAGG GGGCTGCTCT CAATCACCTC 
TCCTCTCACA CTGCTACCTC AGGTGGACAA CAAGGCATTA CCTTAACCAA AGAGAGCAAG 
CCTTCAGGAA ACATATTGAC GGTGCCTGAA ACAAGCAGGC ACACTGGAGA GACACCTAAC 
AGCACTGCCA GTGTCGAGGG ACTTCCTAAT CATGTCCATC AGATGACGGC AGATGCTGTT 
TGCAGTCCTA GCCATGGAGA TTCTAAGTCA CCAGGTTTAC TAAGTTCAGA CAATCCTCAG 
CTCTCTGCCT TGTTGATGGG AAAAGCCAAT AACAATGTGG GTACTGGAAC CTGTGACAAA 
GTCAATAACA TCCACCCAGC TGTTCATACA AAGACTGATA ACTCTGTTGC CTCTTCACCA 
TCTTCAGCCA TTTCAACAGC AACACCTTCT CCAAAATCCA CTGAGCAGAC AACCACAAAC 
AGTGTTACCA GCCTTAACAG CCCTCACAGT GGGCTACACA CAATTAATGG AGAAGGGATG 
GAAGAATCTC AGAGCCCCAT GAAAACAGAT CTGCTTCTGG TTAACCACAA ACCTAGTCCA 
CAGATCATAC CATCAATGTC TGTGTCCATA TACCCCAGCT CAGCAGAAGT TCTGAAGGCA 
TGCAGGAATC TAGGTAAAAA TGGCTTATCT AACAGTAGCA TTTTGTTGGA TAAATGTCCA 
CCTCCAAGAC CACCATCTTC ACCATACCCT CCCTTGCCAA AGGACAAGTT GAATCCACCT 
ACACCTAGTA TTTACTTGGA AAATAAACGT GATGCTTTCT TTCCTCCATT ACATCAATTT 
TGTACAAATC CGAACAACCC TGTTACAGTA ATACGTGGCC TTGCTGGAGC TCTTAAGTTA 
GACCTGGGAC TTTTCTCTAC TAAAACTTTG GTGGAAGCTA ACAATGAACA TATGGTAGAA 
GTGAGGACAC AGTTGTTGCA GCCAGCAGAT GAAAACTGGG ATCCCACTGG AACAAAGAAA 
ATCTGGCATT GTGAAAGTAA TAGATCTCAT ACTACAATTG CTAAATATGC ACAGTACCAG 
GCCTCCTCAT TCCAGGAATC ATTGAGAGAA GAAAATGAAA AAAGAAGTCA TCATAAAGAC 
CACTCAGATA GTGAATCTAC ATCGTCAGAT AATTCTGGGA GGAGGAGGAA AGGACCCTTT 
AAAACCATAA AGTTTGGGAC CAATATTGAC CTATCTGATG ACAAAAAGTG GAAGTTGCAG 
CTACATGAGC TGACTAAACT TCCTGCTTTT GTGCGTGTCG TATCAGCAGG AAATCTTCTA 
AGCCATGTTG GTCATACCAT ATTGGGCATG AACACAGTTC AACTATACAT GAAAGTTCCA 
GGGAGCAGAA CACCAGGTCA TCAGGAAAAT AACAACTTCT GTTCAGTTAA CATAAATATT 
GGCCCAGGTG ACTGTGAATG GTTTGTTGTT CCTGAAGGTT ACTGGGGTGT TCTGAATGAC 
TTCTGTGAAA AAAATAATTT GAATTTCCTA ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT 
CTTTATGAAG CAAATGTTCC AGTGTATAGG TTTATTCAGC GACCTGGAGA TTTGGTCTGG 
ATAAATGCAG GCACTGTTCA TTGGGTTCAG GCTATTGGCT GGTGCAACAA CATTGCTTGG 
AATGTTGGTC CACTTACAGC CTGCCAGTAT AAATTGGCAG TGGAACGGTA CGAATGGAAC 
AAATTGCAAA GTGTGAAGTC AATAGTACCC ATGGTTCATC TTTCCTGGAA TATGGCACGA 
AATATCAAGG TCTCAGATCC AAAGCTTTTT GAAATGATTA AGATATTTTC ATTGTCTCCG 
AATTTTAGAG CTGAAAAGTG CCTTAGAGAT CATCTAGTTC AACCTCTCCG TTCAAATGGA 
GAACCTGAGC CACTAAGATT CACAGGAGAG TATTGTCTTC TAAGAACTCT GAAGCAATGT 
CAGACATTGA GGGAAGCTCT CATTGCTGCA GGAAAAGAGA TTATATGGCA TGGGCGGACA 
AAAGAAGAAC CAGCTCATTA CTGTAGCATT TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT 
GTCACTAATG AGAGTAATTC ACGAAAGACC TACATAGTAC ATTGCCAAGA TTGTGCACGA 
AAAACAAGCG GAAACTTGGA AAACTTTGTG GTGCTAGAAC AGTACAAAAT GGAGGACCTG 
ATGCAAGTCT ATGACCAATT TACATTAGCT CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291417.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278346.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu24587
Clone ID Related Accession (Same CDS sequence)	NM_001291417.1
Accession Version	NM_001291417.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143278.1, AK303430.1, AL831996.2, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced XM_005272660.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.7609.1, SRR1660803.146765.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278346.1
CDS	378..4448
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291417.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291417.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291416.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278345.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24588
Clone ID Related Accession (Same CDS sequence)	NM_001291416.1
Accession Version	NM_001291416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143272.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. On Apr 12, 2014 this sequence version replaced XM_005272657.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.259648.1, SRR1803611.5332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT 
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278345.1
CDS	378..4604
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291416.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291416.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT 
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( NM_001291415.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278344.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.

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CloneID	OHu24601
Clone ID Related Accession (Same CDS sequence)	NM_001291415.2 , NM_001291415.1
Accession Version	NM_001291415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381812.1, BC143277.1, BC143278.1, AL831996.2, AL041377.1 and AI364446.1. This sequence is a reference standard in the RefSeqGene project. On Apr 12, 2014 this sequence version replaced XM_005272655.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.126238.1, SRR1803616.152585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278344.1
CDS	378..4739
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001291415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291415.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_005272656.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272713.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.

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CloneID	OHu54022
Clone ID Related Accession (Same CDS sequence)	XM_005272656.5 , XM_005272656.4
Accession Version	XM_005272656.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272656.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT 
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA 
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272713.1
CDS	371..4630
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_005272656.5
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272656.5

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT 
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA 
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_005272656.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272713.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu54022
Clone ID Related Accession (Same CDS sequence)	XM_005272656.5 , XM_005272656.4
Accession Version	XM_005272656.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272656.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT 
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA 
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272713.1
CDS	378..4637
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_005272656.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272656.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GATGAGACCA ACAGGAGTTG CACAGGTACG ATCTACTGGA 
ATTCCTAATG GGCCAACAGC TGACTCATCA CTGCCTACAA ACTCAGTCTC TGGCCAGCAG 
CCACAGCTTG CTCTGACCAG AGTGCCTAGC GTCTCTCAGC CTGGAGTCCG TCCTGCCTGC 
CCTGGGCAGC CTTTGGCCAA TGGACCCTTT TCTGCAGGCC ATGTTCCCTG TAGCACATCA 
AGAACGCTGG GAAGTACAGA CACTATTTTG ATAGGCAATA ATCATATAAC AGGAAGTGGA 
AGTAATGGAA ACGTGCCTTA CCTGCAGCGA AACGCACTCA CTCTACCTCA TAACCGCACA 
AACCTGACCA GCAGCGCAGA GGAGCCGTGG AAAAACCAAC TATCTAACTC CACTCAGGGG 
CTTCACAAAG GTCAGAGTTC ACATTCGGCA GGTCCTAATG GTGAACGACC TCTCTCTTCC 
ACTGGGCCTT CCCAGCATCT CCAGGCAGCT GGCTCTGGTA TTCAGAATCA GAACGGACAT 
CCCACCCTGC CTAGCAATTC AGTAACACAG GGGGCTGCTC TCAATCACCT CTCCTCTCAC 
ACTGCTACCT CAGGTGGACA ACAAGGCATT ACCTTAACCA AAGAGAGCAA GCCTTCAGGA 
AACATATTGA CGGTGCCTGA AACAAGCAGG CACACTGGAG AGACACCTAA CAGCACTGCC 
AGTGTCGAGG GACTTCCTAA TCATGTCCAT CAGATGACGG CAGATGCTGT TTGCAGTCCT 
AGCCATGGAG ATTCTAAGTC ACCAGGTTTA CTAAGTTCAG ACAATCCTCA GCTCTCTGCC 
TTGTTGATGG GAAAAGCCAA TAACAATGTG GGTACTGGAA CCTGTGACAA AGTCAATAAC 
ATCCACCCAG CTGTTCATAC AAAGACTGAT AACTCTGTTG CCTCTTCACC ATCTTCAGCC 
ATTTCAACAG CAACACCTTC TCCAAAATCC ACTGAGCAGA CAACCACAAA CAGTGTTACC 
AGCCTTAACA GCCCTCACAG TGGGCTACAC ACAATTAATG GAGAAGGGAT GGAAGAATCT 
CAGAGCCCCA TGAAAACAGA TCTGCTTCTG GTTAACCACA AACCTAGTCC ACAGATCATA 
CCATCAATGT CTGTGTCCAT ATACCCCAGC TCAGCAGAAG TTCTGAAGGC ATGCAGGAAT 
CTAGGTAAAA ATGGCTTATC TAACAGTAGC ATTTTGTTGG ATAAATGTCC ACCTCCAAGA 
CCACCATCTT CACCATACCC TCCCTTGCCA AAGGACAAGT TGAATCCACC TACACCTAGT 
ATTTACTTGG AAAATAAACG TGATGCTTTC TTTCCTCCAT TACATCAATT TTGTACAAAT 
CCGAACAACC CTGTTACAGT AATACGTGGC CTTGCTGGAG CTCTTAAGTT AGACCTGGGA 
CTTTTCTCTA CTAAAACTTT GGTGGAAGCT AACAATGAAC ATATGGTAGA AGTGAGGACA 
CAGTTGTTGC AGCCAGCAGA TGAAAACTGG GATCCCACTG GAACAAAGAA AATCTGGCAT 
TGTGAAAGTA ATAGATCTCA TACTACAATT GCTAAATATG CACAGTACCA GGCCTCCTCA 
TTCCAGGAAT CATTGAGAGA AGAAAATGAA AAAAGAAGTC ATCATAAAGA CCACTCAGAT 
AGTGAATCTA CATCGTCAGA TAATTCTGGG AGGAGGAGGA AAGGACCCTT TAAAACCATA 
AAGTTTGGGA CCAATATTGA CCTATCTGAT GACAAAAAGT GGAAGTTGCA GCTACATGAG 
CTGACTAAAC TTCCTGCTTT TGTGCGTGTC GTATCAGCAG GAAATCTTCT AAGCCATGTT 
GGTCATACCA TATTGGGCAT GAACACAGTT CAACTATACA TGAAAGTTCC AGGGAGCAGA 
ACACCAGGTC ATCAGGAAAA TAACAACTTC TGTTCAGTTA ACATAAATAT TGGCCCAGGT 
GACTGTGAAT GGTTTGTTGT TCCTGAAGGT TACTGGGGTG TTCTGAATGA CTTCTGTGAA 
AAAAATAATT TGAATTTCCT AATGGGTTCT TGGTGGCCCA ATCTTGAAGA TCTTTATGAA 
GCAAATGTTC CAGTGTATAG GTTTATTCAG CGACCTGGAG ATTTGGTCTG GATAAATGCA 
GGCACTGTTC ATTGGGTTCA GGCTATTGGC TGGTGCAACA ACATTGCTTG GAATGTTGGT 
CCACTTACAG CCTGCCAGTA TAAATTGGCA GTGGAACGGT ACGAATGGAA CAAATTGCAA 
AGTGTGAAGT CAATAGTACC CATGGTTCAT CTTTCCTGGA ATATGGCACG AAATATCAAG 
GTCTCAGATC CAAAGCTTTT TGAAATGATT AAGTATTGTC TTCTAAGAAC TCTGAAGCAA 
TGTCAGACAT TGAGGGAAGC TCTCATTGCT GCAGGAAAAG AGATTATATG GCATGGGCGG 
ACAAAAGAAG AACCAGCTCA TTACTGTAGC ATTTGTGAAG TGGAGGTTTT TGATCTGCTT 
TTTGTCACTA ATGAGAGTAA TTCACGAAAG ACCTACATAG TACATTGCCA AGATTGTGCA 
CGAAAAACAA GCGGAAACTT GGAAAACTTT GTGGTGCTAG AACAGTACAA AATGGAGGAC 
CTGATGCAAG TCTATGACCA ATTTACATTA GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_005272659.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272716.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu54024
Clone ID Related Accession (Same CDS sequence)	XM_005272659.4 , XM_005272659.5
Accession Version	XM_005272659.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272716.1
CDS	378..4481
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_005272659.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272659.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_005272659.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272716.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.

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CloneID	OHu54024
Clone ID Related Accession (Same CDS sequence)	XM_005272659.4 , XM_005272659.5
Accession Version	XM_005272659.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272659.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272716.1
CDS	371..4474
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_005272659.5
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272659.5

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGTAT 
TGTCTTCTAA GAACTCTGAA GCAATGTCAG ACATTGAGGG AAGCTCTCAT TGCTGCAGGA 
AAAGAGATTA TATGGCATGG GCGGACAAAA GAAGAACCAG CTCATTACTG TAGCATTTGT 
GAAGTGGAGG TTTTTGATCT GCTTTTTGTC ACTAATGAGA GTAATTCACG AAAGACCTAC 
ATAGTACATT GCCAAGATTG TGCACGAAAA ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG 
CTAGAACAGT ACAAAATGGA GGACCTGATG CAAGTCTATG ACCAATTTAC ATTAGCTCCT 
CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543957.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542259.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61248
Clone ID Related Accession (Same CDS sequence)	XM_011543957.2
Accession Version	XM_011543957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA 
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA 
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT 
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA 
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT 
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC 
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC 
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG 
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA 
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC 
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA 
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA 
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA 
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT 
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA 
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT 
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA 
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT 
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT 
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT 
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA 
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT 
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT 
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT 
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA 
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG 
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT 
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT 
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA 
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT 
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC 
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT 
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG 
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT 
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT 
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA 
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG 
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG 
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT 
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC 
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT 
GAAATGATTA AGATATTTTC ATTGTCTCCG AATTTTAGAG CTGAAAAGTG CCTTAGAGAT 
CATCTAGTTC AACCTCTCCG TTCAAATGGA GAACCTGAGC CACTAAGATT CACAGGAGAG 
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT 
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542259.1
CDS	378..4904
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543957.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543957.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA 
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA 
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT 
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA 
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT 
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC 
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC 
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG 
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA 
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC 
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA 
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA 
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA 
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT 
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA 
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT 
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA 
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT 
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT 
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT 
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA 
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT 
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT 
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT 
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA 
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG 
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT 
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT 
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA 
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT 
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC 
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT 
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG 
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT 
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT 
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA 
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG 
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG 
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT 
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC 
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT 
GAAATGATTA AGATATTTTC ATTGTCTCCG AATTTTAGAG CTGAAAAGTG CCTTAGAGAT 
CATCTAGTTC AACCTCTCCG TTCAAATGGA GAACCTGAGC CACTAAGATT CACAGGAGAG 
TATTGTCTTC TAAGAACTCT GAAGCAATGT CAGACATTGA GGGAAGCTCT CATTGCTGCA 
GGAAAAGAGA TTATATGGCA TGGGCGGACA AAAGAAGAAC CAGCTCATTA CTGTAGCATT 
TGTGAAGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC ACGAAAGACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGCG GAAACTTGGA AAACTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGACCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543958.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542260.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61249
Clone ID Related Accession (Same CDS sequence)	XM_011543958.2 , XM_011543958.3
Accession Version	XM_011543958.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542260.1
CDS	378..4847
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543958.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543958.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543958.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542260.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

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CloneID	OHu61249
Clone ID Related Accession (Same CDS sequence)	XM_011543958.2 , XM_011543958.3
Accession Version	XM_011543958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542260.1
CDS	371..4840
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543958.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543958.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGG CATGTAAACC TCATCATCCA AATACTGAAC CTGTATTAGG CCTCAGTCAA 
ACACCAATTT CACAGCAATC CTTGCCACTA CACATGATTC CTTCTAGCCA AGTAGATGAC 
CTGTCCAGTC CTGCCAAGAG GAAAAGAACA TCTAGTCCAA CAAAGAATAC TTCTGACAAT 
TGGAGTGGTG GACATGCTGT GTCACATCCT CCAGTACAGC AACAAGCTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCATTTGGAA CAGCTCCGCG CAAATAGAAA TAATTTAAAT 
CCAGCACAGA AACTGATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAACAACAC 
CAAATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543959.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542261.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

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CloneID	OHu61250
Clone ID Related Accession (Same CDS sequence)	XM_011543959.2
Accession Version	XM_011543959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT 
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA 
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT 
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA 
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT 
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC 
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT 
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT 
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC 
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT 
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC 
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT 
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC 
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG 
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC 
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT 
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC 
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG 
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA 
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA 
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA 
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT 
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG 
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT 
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG 
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT 
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC 
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT 
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG 
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG 
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT 
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA 
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC 
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA 
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA 
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC 
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA 
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT 
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC 
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT 
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA 
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542261.1
CDS	378..4802
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543959.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543959.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGAT GAGACCAACA GGAGTTGCAC AGGTACGATC TACTGGAATT 
CCTAATGGGC CAACAGCTGA CTCATCACTG CCTACAAACT CAGTCTCTGG CCAGCAGCCA 
CAGCTTGCTC TGACCAGAGT GCCTAGCGTC TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT 
GGGCAGCCTT TGGCCAATGG ACCCTTTTCT GCAGGCCATG TTCCCTGTAG CACATCAAGA 
ACGCTGGGAA GTACAGACAC TATTTTGATA GGCAATAATC ATATAACAGG AAGTGGAAGT 
AATGGAAACG TGCCTTACCT GCAGCGAAAC GCACTCACTC TACCTCATAA CCGCACAAAC 
CTGACCAGCA GCGCAGAGGA GCCGTGGAAA AACCAACTAT CTAACTCCAC TCAGGGGCTT 
CACAAAGGTC AGAGTTCACA TTCGGCAGGT CCTAATGGTG AACGACCTCT CTCTTCCACT 
GGGCCTTCCC AGCATCTCCA GGCAGCTGGC TCTGGTATTC AGAATCAGAA CGGACATCCC 
ACCCTGCCTA GCAATTCAGT AACACAGGGG GCTGCTCTCA ATCACCTCTC CTCTCACACT 
GCTACCTCAG GTGGACAACA AGGCATTACC TTAACCAAAG AGAGCAAGCC TTCAGGAAAC 
ATATTGACGG TGCCTGAAAC AAGCAGGCAC ACTGGAGAGA CACCTAACAG CACTGCCAGT 
GTCGAGGGAC TTCCTAATCA TGTCCATCAG ATGACGGCAG ATGCTGTTTG CAGTCCTAGC 
CATGGAGATT CTAAGTCACC AGGTTTACTA AGTTCAGACA ATCCTCAGCT CTCTGCCTTG 
TTGATGGGAA AAGCCAATAA CAATGTGGGT ACTGGAACCT GTGACAAAGT CAATAACATC 
CACCCAGCTG TTCATACAAA GACTGATAAC TCTGTTGCCT CTTCACCATC TTCAGCCATT 
TCAACAGCAA CACCTTCTCC AAAATCCACT GAGCAGACAA CCACAAACAG TGTTACCAGC 
CTTAACAGCC CTCACAGTGG GCTACACACA ATTAATGGAG AAGGGATGGA AGAATCTCAG 
AGCCCCATGA AAACAGATCT GCTTCTGGTT AACCACAAAC CTAGTCCACA GATCATACCA 
TCAATGTCTG TGTCCATATA CCCCAGCTCA GCAGAAGTTC TGAAGGCATG CAGGAATCTA 
GGTAAAAATG GCTTATCTAA CAGTAGCATT TTGTTGGATA AATGTCCACC TCCAAGACCA 
CCATCTTCAC CATACCCTCC CTTGCCAAAG GACAAGTTGA ATCCACCTAC ACCTAGTATT 
TACTTGGAAA ATAAACGTGA TGCTTTCTTT CCTCCATTAC ATCAATTTTG TACAAATCCG 
AACAACCCTG TTACAGTAAT ACGTGGCCTT GCTGGAGCTC TTAAGTTAGA CCTGGGACTT 
TTCTCTACTA AAACTTTGGT GGAAGCTAAC AATGAACATA TGGTAGAAGT GAGGACACAG 
TTGTTGCAGC CAGCAGATGA AAACTGGGAT CCCACTGGAA CAAAGAAAAT CTGGCATTGT 
GAAAGTAATA GATCTCATAC TACAATTGCT AAATATGCAC AGTACCAGGC CTCCTCATTC 
CAGGAATCAT TGAGAGAAGA AAATGAAAAA AGAAGTCATC ATAAAGACCA CTCAGATAGT 
GAATCTACAT CGTCAGATAA TTCTGGGAGG AGGAGGAAAG GACCCTTTAA AACCATAAAG 
TTTGGGACCA ATATTGACCT ATCTGATGAC AAAAAGTGGA AGTTGCAGCT ACATGAGCTG 
ACTAAACTTC CTGCTTTTGT GCGTGTCGTA TCAGCAGGAA ATCTTCTAAG CCATGTTGGT 
CATACCATAT TGGGCATGAA CACAGTTCAA CTATACATGA AAGTTCCAGG GAGCAGAACA 
CCAGGTCATC AGGAAAATAA CAACTTCTGT TCAGTTAACA TAAATATTGG CCCAGGTGAC 
TGTGAATGGT TTGTTGTTCC TGAAGGTTAC TGGGGTGTTC TGAATGACTT CTGTGAAAAA 
AATAATTTGA ATTTCCTAAT GGGTTCTTGG TGGCCCAATC TTGAAGATCT TTATGAAGCA 
AATGTTCCAG TGTATAGGTT TATTCAGCGA CCTGGAGATT TGGTCTGGAT AAATGCAGGC 
ACTGTTCATT GGGTTCAGGC TATTGGCTGG TGCAACAACA TTGCTTGGAA TGTTGGTCCA 
CTTACAGCCT GCCAGTATAA ATTGGCAGTG GAACGGTACG AATGGAACAA ATTGCAAAGT 
GTGAAGTCAA TAGTACCCAT GGTTCATCTT TCCTGGAATA TGGCACGAAA TATCAAGGTC 
TCAGATCCAA AGCTTTTTGA AATGATTAAG ATATTTTCAT TGTCTCCGAA TTTTAGAGCT 
GAAAAGTGCC TTAGAGATCA TCTAGTTCAA CCTCTCCGTT CAAATGGAGA ACCTGAGCCA 
CTAAGATTCA CAGGAGAGTA TTGTCTTCTA AGAACTCTGA AGCAATGTCA GACATTGAGG 
GAAGCTCTCA TTGCTGCAGG AAAAGAGATT ATATGGCATG GGCGGACAAA AGAAGAACCA 
GCTCATTACT GTAGCATTTG TGAAGTGGAG GTTTTTGATC TGCTTTTTGT CACTAATGAG 
AGTAATTCAC GAAAGACCTA CATAGTACAT TGCCAAGATT GTGCACGAAA AACAAGCGGA 
AACTTGGAAA ACTTTGTGGT GCTAGAACAG TACAAAATGG AGGACCTGAT GCAAGTCTAT 
GACCAATTTA CATTAGCTCC TCCATTACCA TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543960.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542262.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu61251
Clone ID Related Accession (Same CDS sequence)	XM_011543960.2
Accession Version	XM_011543960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA 
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA 
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT 
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA 
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT 
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC 
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC 
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG 
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA 
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC 
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA 
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA 
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA 
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT 
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA 
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT 
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA 
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT 
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT 
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT 
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA 
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT 
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT 
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT 
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA 
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG 
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT 
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT 
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA 
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT 
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC 
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT 
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG 
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT 
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT 
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA 
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG 
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG 
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT 
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC 
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT 
GAAATGATTA AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542262.1
CDS	378..4796
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543960.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543960.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGGCAT GTAAACCTCA TCATCCAAAT ACTGAACCTG TATTAGGCCT CAGTCAAACA 
CCAATTTCAC AGCAATCCTT GCCACTACAC ATGATTCCTT CTAGCCAAGT AGATGACCTG 
TCCAGTCCTG CCAAGAGGAA AAGAACATCT AGTCCAACAA AGAATACTTC TGACAATTGG 
AGTGGTGGAC ATGCTGTGTC ACATCCTCCA GTACAGCAAC AAGCTCATTC ATGGTGTTTG 
ACACCACAGA AATTACAGCA TTTGGAACAG CTCCGCGCAA ATAGAAATAA TTTAAATCCA 
GCACAGAAAC TGATGCTGGA ACAGCTGGAA AGTCAGTTTG TCTTAATGCA ACAACACCAA 
ATGAGACCAA CAGGAGTTGC ACAGGTACGA TCTACTGGAA TTCCTAATGG GCCAACAGCT 
GACTCATCAC TGCCTACAAA CTCAGTCTCT GGCCAGCAGC CACAGCTTGC TCTGACCAGA 
GTGCCTAGCG TCTCTCAGCC TGGAGTCCGT CCTGCCTGCC CTGGGCAGCC TTTGGCCAAT 
GGACCCTTTT CTGCAGGCCA TGTTCCCTGT AGCACATCAA GAACGCTGGG AAGTACAGAC 
ACTATTTTGA TAGGCAATAA TCATATAACA GGAAGTGGAA GTAATGGAAA CGTGCCTTAC 
CTGCAGCGAA ACGCACTCAC TCTACCTCAT AACCGCACAA ACCTGACCAG CAGCGCAGAG 
GAGCCGTGGA AAAACCAACT ATCTAACTCC ACTCAGGGGC TTCACAAAGG TCAGAGTTCA 
CATTCGGCAG GTCCTAATGG TGAACGACCT CTCTCTTCCA CTGGGCCTTC CCAGCATCTC 
CAGGCAGCTG GCTCTGGTAT TCAGAATCAG AACGGACATC CCACCCTGCC TAGCAATTCA 
GTAACACAGG GGGCTGCTCT CAATCACCTC TCCTCTCACA CTGCTACCTC AGGTGGACAA 
CAAGGCATTA CCTTAACCAA AGAGAGCAAG CCTTCAGGAA ACATATTGAC GGTGCCTGAA 
ACAAGCAGGC ACACTGGAGA GACACCTAAC AGCACTGCCA GTGTCGAGGG ACTTCCTAAT 
CATGTCCATC AGATGACGGC AGATGCTGTT TGCAGTCCTA GCCATGGAGA TTCTAAGTCA 
CCAGGTTTAC TAAGTTCAGA CAATCCTCAG CTCTCTGCCT TGTTGATGGG AAAAGCCAAT 
AACAATGTGG GTACTGGAAC CTGTGACAAA GTCAATAACA TCCACCCAGC TGTTCATACA 
AAGACTGATA ACTCTGTTGC CTCTTCACCA TCTTCAGCCA TTTCAACAGC AACACCTTCT 
CCAAAATCCA CTGAGCAGAC AACCACAAAC AGTGTTACCA GCCTTAACAG CCCTCACAGT 
GGGCTACACA CAATTAATGG AGAAGGGATG GAAGAATCTC AGAGCCCCAT GAAAACAGAT 
CTGCTTCTGG TTAACCACAA ACCTAGTCCA CAGATCATAC CATCAATGTC TGTGTCCATA 
TACCCCAGCT CAGCAGAAGT TCTGAAGGCA TGCAGGAATC TAGGTAAAAA TGGCTTATCT 
AACAGTAGCA TTTTGTTGGA TAAATGTCCA CCTCCAAGAC CACCATCTTC ACCATACCCT 
CCCTTGCCAA AGGACAAGTT GAATCCACCT ACACCTAGTA TTTACTTGGA AAATAAACGT 
GATGCTTTCT TTCCTCCATT ACATCAATTT TGTACAAATC CGAACAACCC TGTTACAGTA 
ATACGTGGCC TTGCTGGAGC TCTTAAGTTA GACCTGGGAC TTTTCTCTAC TAAAACTTTG 
GTGGAAGCTA ACAATGAACA TATGGTAGAA GTGAGGACAC AGTTGTTGCA GCCAGCAGAT 
GAAAACTGGG ATCCCACTGG AACAAAGAAA ATCTGGCATT GTGAAAGTAA TAGATCTCAT 
ACTACAATTG CTAAATATGC ACAGTACCAG GCCTCCTCAT TCCAGGAATC ATTGAGAGAA 
GAAAATGAAA AAAGAAGTCA TCATAAAGAC CACTCAGATA GTGAATCTAC ATCGTCAGAT 
AATTCTGGGA GGAGGAGGAA AGGACCCTTT AAAACCATAA AGTTTGGGAC CAATATTGAC 
CTATCTGATG ACAAAAAGTG GAAGTTGCAG CTACATGAGC TGACTAAACT TCCTGCTTTT 
GTGCGTGTCG TATCAGCAGG AAATCTTCTA AGCCATGTTG GTCATACCAT ATTGGGCATG 
AACACAGTTC AACTATACAT GAAAGTTCCA GGGAGCAGAA CACCAGGTCA TCAGGAAAAT 
AACAACTTCT GTTCAGTTAA CATAAATATT GGCCCAGGTG ACTGTGAATG GTTTGTTGTT 
CCTGAAGGTT ACTGGGGTGT TCTGAATGAC TTCTGTGAAA AAAATAATTT GAATTTCCTA 
ATGGGTTCTT GGTGGCCCAA TCTTGAAGAT CTTTATGAAG CAAATGTTCC AGTGTATAGG 
TTTATTCAGC GACCTGGAGA TTTGGTCTGG ATAAATGCAG GCACTGTTCA TTGGGTTCAG 
GCTATTGGCT GGTGCAACAA CATTGCTTGG AATGTTGGTC CACTTACAGC CTGCCAGTAT 
AAATTGGCAG TGGAACGGTA CGAATGGAAC AAATTGCAAA GTGTGAAGTC AATAGTACCC 
ATGGTTCATC TTTCCTGGAA TATGGCACGA AATATCAAGG TCTCAGATCC AAAGCTTTTT 
GAAATGATTA AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543961.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542263.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu61252
Clone ID Related Accession (Same CDS sequence)	XM_011543961.2
Accession Version	XM_011543961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG 
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542263.1
CDS	378..4769
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543961.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGCATTTGG AACAGCTCCG CGCAAATAGA AATAATTTAA ATCCAGCACA GAAACTGATG 
CTGGAACAGC TGGAAAGTCA GTTTGTCTTA ATGCAACAAC ACCAAATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543962.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542264.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.

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CloneID	OHu61253
Clone ID Related Accession (Same CDS sequence)	XM_011543962.2
Accession Version	XM_011543962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC 
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT 
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG 
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC 
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT 
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA 
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC 
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG 
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA 
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG 
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC 
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA 
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC 
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT 
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT 
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC 
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT 
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG 
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGATAT TTTCATTGTC TCCGAATTTT 
AGAGCTGAAA AGTGCCTTAG AGATCATCTA GTTCAACCTC TCCGTTCAAA TGGAGAACCT 
GAGCCACTAA GATTCACAGG AGAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542264.1
CDS	378..4748
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543962.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543962.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC 
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT 
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG 
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC 
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT 
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA 
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC 
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG 
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA 
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG 
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC 
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA 
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC 
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT 
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT 
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC 
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT 
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG 
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGATAT TTTCATTGTC TCCGAATTTT 
AGAGCTGAAA AGTGCCTTAG AGATCATCTA GTTCAACCTC TCCGTTCAAA TGGAGAACCT 
GAGCCACTAA GATTCACAGG AGAGTATTGT CTTCTAAGAA CTCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTCATTGC TGCAGGAAAA GAGATTATAT GGCATGGGCG GACAAAAGAA 
GAACCAGCTC ATTACTGTAG CATTTGTGAA GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCACGAAA GACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGCGGAAACT TGGAAAACTT TGTGGTGCTA GAACAGTACA AAATGGAGGA CCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543963.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542265.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.

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CloneID	OHu61254
Clone ID Related Accession (Same CDS sequence)	XM_011543963.2 , XM_011543963.3
Accession Version	XM_011543963.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA 
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA 
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT 
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG 
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT 
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG 
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA 
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542265.1
CDS	378..4712
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543963.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543963.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA 
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA 
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT 
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG 
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT 
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG 
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA 
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543963.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542265.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu61254
Clone ID Related Accession (Same CDS sequence)	XM_011543963.2 , XM_011543963.3
Accession Version	XM_011543963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA 
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA 
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT 
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG 
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT 
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG 
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA 
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542265.1
CDS	371..4705
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543963.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543963.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCATT TGGAACAGCT CCGCGCAAAT AGAAATAATT TAAATCCAGC ACAGAAACTG 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAAC AACACCAAAT GAGACCAACA 
GGAGTTGCAC AGGTACGATC TACTGGAATT CCTAATGGGC CAACAGCTGA CTCATCACTG 
CCTACAAACT CAGTCTCTGG CCAGCAGCCA CAGCTTGCTC TGACCAGAGT GCCTAGCGTC 
TCTCAGCCTG GAGTCCGTCC TGCCTGCCCT GGGCAGCCTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACGCTGGGAA GTACAGACAC TATTTTGATA 
GGCAATAATC ATATAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACTCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCGCAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC AGAGTTCACA TTCGGCAGGT 
CCTAATGGTG AACGACCTCT CTCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA CGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ATATTGACGG TGCCTGAAAC AAGCAGGCAC 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTCGAGGGAC TTCCTAATCA TGTCCATCAG 
ATGACGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCCAATAA CAATGTGGGT 
ACTGGAACCT GTGACAAAGT CAATAACATC CACCCAGCTG TTCATACAAA GACTGATAAC 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCAACAGCAA CACCTTCTCC AAAATCCACT 
GAGCAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTACACACA 
ATTAATGGAG AAGGGATGGA AGAATCTCAG AGCCCCATGA AAACAGATCT GCTTCTGGTT 
AACCACAAAC CTAGTCCACA GATCATACCA TCAATGTCTG TGTCCATATA CCCCAGCTCA 
GCAGAAGTTC TGAAGGCATG CAGGAATCTA GGTAAAAATG GCTTATCTAA CAGTAGCATT 
TTGTTGGATA AATGTCCACC TCCAAGACCA CCATCTTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TACTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAATCCG AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAGTTAGA CCTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTGCAGC CAGCAGATGA AAACTGGGAT 
CCCACTGGAA CAAAGAAAAT CTGGCATTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCAC AGTACCAGGC CTCCTCATTC CAGGAATCAT TGAGAGAAGA AAATGAAAAA 
AGAAGTCATC ATAAAGACCA CTCAGATAGT GAATCTACAT CGTCAGATAA TTCTGGGAGG 
AGGAGGAAAG GACCCTTTAA AACCATAAAG TTTGGGACCA ATATTGACCT ATCTGATGAC 
AAAAAGTGGA AGTTGCAGCT ACATGAGCTG ACTAAACTTC CTGCTTTTGT GCGTGTCGTA 
TCAGCAGGAA ATCTTCTAAG CCATGTTGGT CATACCATAT TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG GAGCAGAACA CCAGGTCATC AGGAAAATAA CAACTTCTGT 
TCAGTTAACA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGTTAC 
TGGGGTGTTC TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCCTAAT GGGTTCTTGG 
TGGCCCAATC TTGAAGATCT TTATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATT TGGTCTGGAT AAATGCAGGC ACTGTTCATT GGGTTCAGGC TATTGGCTGG 
TGCAACAACA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GCCAGTATAA ATTGGCAGTG 
GAACGGTACG AATGGAACAA ATTGCAAAGT GTGAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTC TCAGATCCAA AGCTTTTTGA AATGATTAAG 
ATATTTTCAT TGTCTCCGAA TTTTAGAGCT GAAAAGTGCC TTAGAGATCA TCTAGTTCAA 
CCTCTCCGTT CAAATGGAGA ACCTGAGCCA CTAAGATTCA CAGGAGAGTA TTGTCTTCTA 
AGAACTCTGA AGCAATGTCA GACATTGAGG GAAGCTCTCA TTGCTGCAGG AAAAGAGATT 
ATATGGCATG GGCGGACAAA AGAAGAACCA GCTCATTACT GTAGCATTTG TGAAGTGGAG 
GTTTTTGATC TGCTTTTTGT CACTAATGAG AGTAATTCAC GAAAGACCTA CATAGTACAT 
TGCCAAGATT GTGCACGAAA AACAAGCGGA AACTTGGAAA ACTTTGTGGT GCTAGAACAG 
TACAAAATGG AGGACCTGAT GCAAGTCTAT GACCAATTTA CATTAGCTCC TCCATTACCA 
TCCGCCTCAT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543964.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542266.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu61255
Clone ID Related Accession (Same CDS sequence)	XM_011543964.2 , XM_011543964.3
Accession Version	XM_011543964.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT 
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA 
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG 
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA 
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC 
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA 
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG 
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542266.1
CDS	378..4691
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543964.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543964.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT 
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA 
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG 
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA 
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC 
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA 
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG 
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543964.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542266.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.

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CloneID	OHu61255
Clone ID Related Accession (Same CDS sequence)	XM_011543964.2 , XM_011543964.3
Accession Version	XM_011543964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT 
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA 
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG 
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA 
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC 
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA 
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG 
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542266.1
CDS	371..4684
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543964.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543964.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCATTT GGAACAGCTC 
CGCGCAAATA GAAATAATTT AAATCCAGCA CAGAAACTGA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAACA ACACCAAATG AGACCAACAG GAGTTGCACA GGTACGATCT 
ACTGGAATTC CTAATGGGCC AACAGCTGAC TCATCACTGC CTACAAACTC AGTCTCTGGC 
CAGCAGCCAC AGCTTGCTCT GACCAGAGTG CCTAGCGTCT CTCAGCCTGG AGTCCGTCCT 
GCCTGCCCTG GGCAGCCTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CGCTGGGAAG TACAGACACT ATTTTGATAG GCAATAATCA TATAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACTCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CGCAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCA GAGTTCACAT TCGGCAGGTC CTAATGGTGA ACGACCTCTC 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAC 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA TATTGACGGT GCCTGAAACA AGCAGGCACA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TCGAGGGACT TCCTAATCAT GTCCATCAGA TGACGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCCAATAAC AATGTGGGTA CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAGCTGT TCATACAAAG ACTGATAACT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CAACAGCAAC ACCTTCTCCA AAATCCACTG AGCAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTACACACAA TTAATGGAGA AGGGATGGAA 
GAATCTCAGA GCCCCATGAA AACAGATCTG CTTCTGGTTA ACCACAAACC TAGTCCACAG 
ATCATACCAT CAATGTCTGT GTCCATATAC CCCAGCTCAG CAGAAGTTCT GAAGGCATGC 
AGGAATCTAG GTAAAAATGG CTTATCTAAC AGTAGCATTT TGTTGGATAA ATGTCCACCT 
CCAAGACCAC CATCTTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ACTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAATCCGA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAGTTAGAC 
CTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTGCAGCC AGCAGATGAA AACTGGGATC CCACTGGAAC AAAGAAAATC 
TGGCATTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCACA GTACCAGGCC 
TCCTCATTCC AGGAATCATT GAGAGAAGAA AATGAAAAAA GAAGTCATCA TAAAGACCAC 
TCAGATAGTG AATCTACATC GTCAGATAAT TCTGGGAGGA GGAGGAAAGG ACCCTTTAAA 
ACCATAAAGT TTGGGACCAA TATTGACCTA TCTGATGACA AAAAGTGGAA GTTGCAGCTA 
CATGAGCTGA CTAAACTTCC TGCTTTTGTG CGTGTCGTAT CAGCAGGAAA TCTTCTAAGC 
CATGTTGGTC ATACCATATT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGG 
AGCAGAACAC CAGGTCATCA GGAAAATAAC AACTTCTGTT CAGTTAACAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGTTACT GGGGTGTTCT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCCTAATG GGTTCTTGGT GGCCCAATCT TGAAGATCTT 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATTT GGTCTGGATA 
AATGCAGGCA CTGTTCATTG GGTTCAGGCT ATTGGCTGGT GCAACAACAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG CCAGTATAAA TTGGCAGTGG AACGGTACGA ATGGAACAAA 
TTGCAAAGTG TGAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTCT CAGATCCAAA GCTTTTTGAA ATGATTAAGA TATTTTCATT GTCTCCGAAT 
TTTAGAGCTG AAAAGTGCCT TAGAGATCAT CTAGTTCAAC CTCTCCGTTC AAATGGAGAA 
CCTGAGCCAC TAAGATTCAC AGGAGAGTAT TGTCTTCTAA GAACTCTGAA GCAATGTCAG 
ACATTGAGGG AAGCTCTCAT TGCTGCAGGA AAAGAGATTA TATGGCATGG GCGGACAAAA 
GAAGAACCAG CTCATTACTG TAGCATTTGT GAAGTGGAGG TTTTTGATCT GCTTTTTGTC 
ACTAATGAGA GTAATTCACG AAAGACCTAC ATAGTACATT GCCAAGATTG TGCACGAAAA 
ACAAGCGGAA ACTTGGAAAA CTTTGTGGTG CTAGAACAGT ACAAAATGGA GGACCTGATG 
CAAGTCTATG ACCAATTTAC ATTAGCTCCT CCATTACCAT CCGCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543965.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542267.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X9, mRNA.

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CloneID	OHu61256
Clone ID Related Accession (Same CDS sequence)	XM_011543965.2
Accession Version	XM_011543965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543965.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542267.1
CDS	378..4667
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543965.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGATATT TTCATTGTCT CCGAATTTTA GAGCTGAAAA GTGCCTTAGA 
GATCATCTAG TTCAACCTCT CCGTTCAAAT GGAGAACCTG AGCCACTAAG ATTCACAGGA 
GAGTATTGTC TTCTAAGAAC TCTGAAGCAA TGTCAGACAT TGAGGGAAGC TCTCATTGCT 
GCAGGAAAAG AGATTATATG GCATGGGCGG ACAAAAGAAG AACCAGCTCA TTACTGTAGC 
ATTTGTGAAG TGGAGGTTTT TGATCTGCTT TTTGTCACTA ATGAGAGTAA TTCACGAAAG 
ACCTACATAG TACATTGCCA AGATTGTGCA CGAAAAACAA GCGGAAACTT GGAAAACTTT 
GTGGTGCTAG AACAGTACAA AATGGAGGAC CTGATGCAAG TCTATGACCA ATTTACATTA 
GCTCCTCCAT TACCATCCGC CTCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543966.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542268.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu61257
Clone ID Related Accession (Same CDS sequence)	XM_011543966.2
Accession Version	XM_011543966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGATGAGACC AACAGGAGTT 
GCACAGGTAC GATCTACTGG AATTCCTAAT GGGCCAACAG CTGACTCATC ACTGCCTACA 
AACTCAGTCT CTGGCCAGCA GCCACAGCTT GCTCTGACCA GAGTGCCTAG CGTCTCTCAG 
CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG CCTTTGGCCA ATGGACCCTT TTCTGCAGGC 
CATGTTCCCT GTAGCACATC AAGAACGCTG GGAAGTACAG ACACTATTTT GATAGGCAAT 
AATCATATAA CAGGAAGTGG AAGTAATGGA AACGTGCCTT ACCTGCAGCG AAACGCACTC 
ACTCTACCTC ATAACCGCAC AAACCTGACC AGCAGCGCAG AGGAGCCGTG GAAAAACCAA 
CTATCTAACT CCACTCAGGG GCTTCACAAA GGTCAGAGTT CACATTCGGC AGGTCCTAAT 
GGTGAACGAC CTCTCTCTTC CACTGGGCCT TCCCAGCATC TCCAGGCAGC TGGCTCTGGT 
ATTCAGAATC AGAACGGACA TCCCACCCTG CCTAGCAATT CAGTAACACA GGGGGCTGCT 
CTCAATCACC TCTCCTCTCA CACTGCTACC TCAGGTGGAC AACAAGGCAT TACCTTAACC 
AAAGAGAGCA AGCCTTCAGG AAACATATTG ACGGTGCCTG AAACAAGCAG GCACACTGGA 
GAGACACCTA ACAGCACTGC CAGTGTCGAG GGACTTCCTA ATCATGTCCA TCAGATGACG 
GCAGATGCTG TTTGCAGTCC TAGCCATGGA GATTCTAAGT CACCAGGTTT ACTAAGTTCA 
GACAATCCTC AGCTCTCTGC CTTGTTGATG GGAAAAGCCA ATAACAATGT GGGTACTGGA 
ACCTGTGACA AAGTCAATAA CATCCACCCA GCTGTTCATA CAAAGACTGA TAACTCTGTT 
GCCTCTTCAC CATCTTCAGC CATTTCAACA GCAACACCTT CTCCAAAATC CACTGAGCAG 
ACAACCACAA ACAGTGTTAC CAGCCTTAAC AGCCCTCACA GTGGGCTACA CACAATTAAT 
GGAGAAGGGA TGGAAGAATC TCAGAGCCCC ATGAAAACAG ATCTGCTTCT GGTTAACCAC 
AAACCTAGTC CACAGATCAT ACCATCAATG TCTGTGTCCA TATACCCCAG CTCAGCAGAA 
GTTCTGAAGG CATGCAGGAA TCTAGGTAAA AATGGCTTAT CTAACAGTAG CATTTTGTTG 
GATAAATGTC CACCTCCAAG ACCACCATCT TCACCATACC CTCCCTTGCC AAAGGACAAG 
TTGAATCCAC CTACACCTAG TATTTACTTG GAAAATAAAC GTGATGCTTT CTTTCCTCCA 
TTACATCAAT TTTGTACAAA TCCGAACAAC CCTGTTACAG TAATACGTGG CCTTGCTGGA 
GCTCTTAAGT TAGACCTGGG ACTTTTCTCT ACTAAAACTT TGGTGGAAGC TAACAATGAA 
CATATGGTAG AAGTGAGGAC ACAGTTGTTG CAGCCAGCAG ATGAAAACTG GGATCCCACT 
GGAACAAAGA AAATCTGGCA TTGTGAAAGT AATAGATCTC ATACTACAAT TGCTAAATAT 
GCACAGTACC AGGCCTCCTC ATTCCAGGAA TCATTGAGAG AAGAAAATGA AAAAAGAAGT 
CATCATAAAG ACCACTCAGA TAGTGAATCT ACATCGTCAG ATAATTCTGG GAGGAGGAGG 
AAAGGACCCT TTAAAACCAT AAAGTTTGGG ACCAATATTG ACCTATCTGA TGACAAAAAG 
TGGAAGTTGC AGCTACATGA GCTGACTAAA CTTCCTGCTT TTGTGCGTGT CGTATCAGCA 
GGAAATCTTC TAAGCCATGT TGGTCATACC ATATTGGGCA TGAACACAGT TCAACTATAC 
ATGAAAGTTC CAGGGAGCAG AACACCAGGT CATCAGGAAA ATAACAACTT CTGTTCAGTT 
AACATAAATA TTGGCCCAGG TGACTGTGAA TGGTTTGTTG TTCCTGAAGG TTACTGGGGT 
GTTCTGAATG ACTTCTGTGA AAAAAATAAT TTGAATTTCC TAATGGGTTC TTGGTGGCCC 
AATCTTGAAG ATCTTTATGA AGCAAATGTT CCAGTGTATA GGTTTATTCA GCGACCTGGA 
GATTTGGTCT GGATAAATGC AGGCACTGTT CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCTT GGAATGTTGG TCCACTTACA GCCTGCCAGT ATAAATTGGC AGTGGAACGG 
TACGAATGGA ACAAATTGCA AAGTGTGAAG TCAATAGTAC CCATGGTTCA TCTTTCCTGG 
AATATGGCAC GAAATATCAA GGTCTCAGAT CCAAAGCTTT TTGAAATGAT TAAGATATTT 
TCATTGTCTC CGAATTTTAG AGCTGAAAAG TGCCTTAGAG ATCATCTAGT TCAACCTCTC 
CGTTCAAATG GAGAACCTGA GCCACTAAGA TTCACAGGAG AGTATTGTCT TCTAAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT 
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA 
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542268.1
CDS	378..4646
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543966.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543966.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGATGAGACC AACAGGAGTT 
GCACAGGTAC GATCTACTGG AATTCCTAAT GGGCCAACAG CTGACTCATC ACTGCCTACA 
AACTCAGTCT CTGGCCAGCA GCCACAGCTT GCTCTGACCA GAGTGCCTAG CGTCTCTCAG 
CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG CCTTTGGCCA ATGGACCCTT TTCTGCAGGC 
CATGTTCCCT GTAGCACATC AAGAACGCTG GGAAGTACAG ACACTATTTT GATAGGCAAT 
AATCATATAA CAGGAAGTGG AAGTAATGGA AACGTGCCTT ACCTGCAGCG AAACGCACTC 
ACTCTACCTC ATAACCGCAC AAACCTGACC AGCAGCGCAG AGGAGCCGTG GAAAAACCAA 
CTATCTAACT CCACTCAGGG GCTTCACAAA GGTCAGAGTT CACATTCGGC AGGTCCTAAT 
GGTGAACGAC CTCTCTCTTC CACTGGGCCT TCCCAGCATC TCCAGGCAGC TGGCTCTGGT 
ATTCAGAATC AGAACGGACA TCCCACCCTG CCTAGCAATT CAGTAACACA GGGGGCTGCT 
CTCAATCACC TCTCCTCTCA CACTGCTACC TCAGGTGGAC AACAAGGCAT TACCTTAACC 
AAAGAGAGCA AGCCTTCAGG AAACATATTG ACGGTGCCTG AAACAAGCAG GCACACTGGA 
GAGACACCTA ACAGCACTGC CAGTGTCGAG GGACTTCCTA ATCATGTCCA TCAGATGACG 
GCAGATGCTG TTTGCAGTCC TAGCCATGGA GATTCTAAGT CACCAGGTTT ACTAAGTTCA 
GACAATCCTC AGCTCTCTGC CTTGTTGATG GGAAAAGCCA ATAACAATGT GGGTACTGGA 
ACCTGTGACA AAGTCAATAA CATCCACCCA GCTGTTCATA CAAAGACTGA TAACTCTGTT 
GCCTCTTCAC CATCTTCAGC CATTTCAACA GCAACACCTT CTCCAAAATC CACTGAGCAG 
ACAACCACAA ACAGTGTTAC CAGCCTTAAC AGCCCTCACA GTGGGCTACA CACAATTAAT 
GGAGAAGGGA TGGAAGAATC TCAGAGCCCC ATGAAAACAG ATCTGCTTCT GGTTAACCAC 
AAACCTAGTC CACAGATCAT ACCATCAATG TCTGTGTCCA TATACCCCAG CTCAGCAGAA 
GTTCTGAAGG CATGCAGGAA TCTAGGTAAA AATGGCTTAT CTAACAGTAG CATTTTGTTG 
GATAAATGTC CACCTCCAAG ACCACCATCT TCACCATACC CTCCCTTGCC AAAGGACAAG 
TTGAATCCAC CTACACCTAG TATTTACTTG GAAAATAAAC GTGATGCTTT CTTTCCTCCA 
TTACATCAAT TTTGTACAAA TCCGAACAAC CCTGTTACAG TAATACGTGG CCTTGCTGGA 
GCTCTTAAGT TAGACCTGGG ACTTTTCTCT ACTAAAACTT TGGTGGAAGC TAACAATGAA 
CATATGGTAG AAGTGAGGAC ACAGTTGTTG CAGCCAGCAG ATGAAAACTG GGATCCCACT 
GGAACAAAGA AAATCTGGCA TTGTGAAAGT AATAGATCTC ATACTACAAT TGCTAAATAT 
GCACAGTACC AGGCCTCCTC ATTCCAGGAA TCATTGAGAG AAGAAAATGA AAAAAGAAGT 
CATCATAAAG ACCACTCAGA TAGTGAATCT ACATCGTCAG ATAATTCTGG GAGGAGGAGG 
AAAGGACCCT TTAAAACCAT AAAGTTTGGG ACCAATATTG ACCTATCTGA TGACAAAAAG 
TGGAAGTTGC AGCTACATGA GCTGACTAAA CTTCCTGCTT TTGTGCGTGT CGTATCAGCA 
GGAAATCTTC TAAGCCATGT TGGTCATACC ATATTGGGCA TGAACACAGT TCAACTATAC 
ATGAAAGTTC CAGGGAGCAG AACACCAGGT CATCAGGAAA ATAACAACTT CTGTTCAGTT 
AACATAAATA TTGGCCCAGG TGACTGTGAA TGGTTTGTTG TTCCTGAAGG TTACTGGGGT 
GTTCTGAATG ACTTCTGTGA AAAAAATAAT TTGAATTTCC TAATGGGTTC TTGGTGGCCC 
AATCTTGAAG ATCTTTATGA AGCAAATGTT CCAGTGTATA GGTTTATTCA GCGACCTGGA 
GATTTGGTCT GGATAAATGC AGGCACTGTT CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCTT GGAATGTTGG TCCACTTACA GCCTGCCAGT ATAAATTGGC AGTGGAACGG 
TACGAATGGA ACAAATTGCA AAGTGTGAAG TCAATAGTAC CCATGGTTCA TCTTTCCTGG 
AATATGGCAC GAAATATCAA GGTCTCAGAT CCAAAGCTTT TTGAAATGAT TAAGATATTT 
TCATTGTCTC CGAATTTTAG AGCTGAAAAG TGCCTTAGAG ATCATCTAGT TCAACCTCTC 
CGTTCAAATG GAGAACCTGA GCCACTAAGA TTCACAGGAG AGTATTGTCT TCTAAGAACT 
CTGAAGCAAT GTCAGACATT GAGGGAAGCT CTCATTGCTG CAGGAAAAGA GATTATATGG 
CATGGGCGGA CAAAAGAAGA ACCAGCTCAT TACTGTAGCA TTTGTGAAGT GGAGGTTTTT 
GATCTGCTTT TTGTCACTAA TGAGAGTAAT TCACGAAAGA CCTACATAGT ACATTGCCAA 
GATTGTGCAC GAAAAACAAG CGGAAACTTG GAAAACTTTG TGGTGCTAGA ACAGTACAAA 
ATGGAGGACC TGATGCAAGT CTATGACCAA TTTACATTAG CTCCTCCATT ACCATCCGCC 
TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543967.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542269.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu61258
Clone ID Related Accession (Same CDS sequence)	XM_011543967.2
Accession Version	XM_011543967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC 
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT 
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG 
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC 
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT 
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA 
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC 
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG 
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA 
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG 
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC 
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA 
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC 
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT 
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT 
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC 
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT 
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG 
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTAAG AACTCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTCATT GCTGCAGGAA AAGAGATTAT ATGGCATGGG 
CGGACAAAAG AAGAACCAGC TCATTACTGT AGCATTTGTG AAGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCACGA AAGACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGCGGAAA CTTGGAAAAC TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GACCTGATGC AAGTCTATGA CCAATTTACA TTAGCTCCTC CATTACCATC CGCCTCATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542269.1
CDS	378..4640
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543967.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543967.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCTCAGTTG 
TGTAACCTTC CACAAGGTAG TCTACAGAAT AAAACTAAAT TACTTCCTAG TATTGAGGAG 
GCGTGGAGCC TACCAATTCC CGCAGAGCTT ACCTCCAGGC AGGGTGCCAT GAACACAGCA 
CAGCAGAATA CTTCTGACAA TTGGAGTGGT GGACATGCTG TGTCACATCC TCCAGTACAG 
CAACAAGCTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCATTTGGA ACAGCTCCGC 
GCAAATAGAA ATAATTTAAA TCCAGCACAG AAACTGATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAACAACA CCAAATGAGA CCAACAGGAG TTGCACAGGT ACGATCTACT 
GGAATTCCTA ATGGGCCAAC AGCTGACTCA TCACTGCCTA CAAACTCAGT CTCTGGCCAG 
CAGCCACAGC TTGCTCTGAC CAGAGTGCCT AGCGTCTCTC AGCCTGGAGT CCGTCCTGCC 
TGCCCTGGGC AGCCTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACGC TGGGAAGTAC AGACACTATT TTGATAGGCA ATAATCATAT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC TCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCGC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCAGAG TTCACATTCG GCAGGTCCTA ATGGTGAACG ACCTCTCTCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAACGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACATAT TGACGGTGCC TGAAACAAGC AGGCACACTG GAGAGACACC TAACAGCACT 
GCCAGTGTCG AGGGACTTCC TAATCATGTC CATCAGATGA CGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC CAATAACAAT GTGGGTACTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAGCTGTTCA TACAAAGACT GATAACTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCAA CAGCAACACC TTCTCCAAAA TCCACTGAGC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTA CACACAATTA ATGGAGAAGG GATGGAAGAA 
TCTCAGAGCC CCATGAAAAC AGATCTGCTT CTGGTTAACC ACAAACCTAG TCCACAGATC 
ATACCATCAA TGTCTGTGTC CATATACCCC AGCTCAGCAG AAGTTCTGAA GGCATGCAGG 
AATCTAGGTA AAAATGGCTT ATCTAACAGT AGCATTTTGT TGGATAAATG TCCACCTCCA 
AGACCACCAT CTTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTACT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AATCCGAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA GTTAGACCTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TGCAGCCAGC AGATGAAAAC TGGGATCCCA CTGGAACAAA GAAAATCTGG 
CATTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCACAGTA CCAGGCCTCC 
TCATTCCAGG AATCATTGAG AGAAGAAAAT GAAAAAAGAA GTCATCATAA AGACCACTCA 
GATAGTGAAT CTACATCGTC AGATAATTCT GGGAGGAGGA GGAAAGGACC CTTTAAAACC 
ATAAAGTTTG GGACCAATAT TGACCTATCT GATGACAAAA AGTGGAAGTT GCAGCTACAT 
GAGCTGACTA AACTTCCTGC TTTTGTGCGT GTCGTATCAG CAGGAAATCT TCTAAGCCAT 
GTTGGTCATA CCATATTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGGAGC 
AGAACACCAG GTCATCAGGA AAATAACAAC TTCTGTTCAG TTAACATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGTTACTGGG GTGTTCTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CCTAATGGGT TCTTGGTGGC CCAATCTTGA AGATCTTTAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATTTGGT CTGGATAAAT 
GCAGGCACTG TTCATTGGGT TCAGGCTATT GGCTGGTGCA ACAACATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGCCA GTATAAATTG GCAGTGGAAC GGTACGAATG GAACAAATTG 
CAAAGTGTGA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTCTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTAAG AACTCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTCATT GCTGCAGGAA AAGAGATTAT ATGGCATGGG 
CGGACAAAAG AAGAACCAGC TCATTACTGT AGCATTTGTG AAGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCACGA AAGACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGCGGAAA CTTGGAAAAC TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GACCTGATGC AAGTCTATGA CCAATTTACA TTAGCTCCTC CATTACCATC CGCCTCATCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543968.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542270.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.

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CloneID	OHu61259
Clone ID Related Accession (Same CDS sequence)	XM_011543968.2
Accession Version	XM_011543968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT 
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA 
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT 
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA 
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG 
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT 
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT 
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA 
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC 
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC 
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT 
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC 
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT 
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA 
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG 
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA 
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT 
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT 
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA 
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC 
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG 
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT 
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT 
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA 
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA 
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT 
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT 
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG 
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT 
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA 
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA 
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC 
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT 
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA 
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT 
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG 
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG 
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA 
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT 
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC 
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA 
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA 
AAGCTTTTTG AAATGATTAA GATATTTTCA TTGTCTCCGA ATTTTAGAGC TGAAAAGTGC 
CTTAGAGATC ATCTAGTTCA ACCTCTCCGT TCAAATGGAG AACCTGAGCC ACTAAGATTC 
ACAGGAGAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC 
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC 
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA 
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA 
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT 
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542270.1
CDS	378..4613
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543968.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543968.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT 
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA 
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT 
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA 
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG 
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT 
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT 
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA 
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC 
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC 
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT 
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC 
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT 
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA 
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG 
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA 
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT 
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT 
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA 
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC 
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG 
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT 
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT 
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA 
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA 
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT 
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT 
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG 
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT 
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA 
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA 
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC 
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT 
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA 
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT 
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG 
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG 
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA 
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT 
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC 
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA 
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA 
AAGCTTTTTG AAATGATTAA GATATTTTCA TTGTCTCCGA ATTTTAGAGC TGAAAAGTGC 
CTTAGAGATC ATCTAGTTCA ACCTCTCCGT TCAAATGGAG AACCTGAGCC ACTAAGATTC 
ACAGGAGAGT ATTGTCTTCT AAGAACTCTG AAGCAATGTC AGACATTGAG GGAAGCTCTC 
ATTGCTGCAG GAAAAGAGAT TATATGGCAT GGGCGGACAA AAGAAGAACC AGCTCATTAC 
TGTAGCATTT GTGAAGTGGA GGTTTTTGAT CTGCTTTTTG TCACTAATGA GAGTAATTCA 
CGAAAGACCT ACATAGTACA TTGCCAAGAT TGTGCACGAA AAACAAGCGG AAACTTGGAA 
AACTTTGTGG TGCTAGAACA GTACAAAATG GAGGACCTGA TGCAAGTCTA TGACCAATTT 
ACATTAGCTC CTCCATTACC ATCCGCCTCA TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543969.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542271.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.

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CloneID	OHu61260
Clone ID Related Accession (Same CDS sequence)	XM_011543969.2 , XM_011543969.3
Accession Version	XM_011543969.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT 
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA 
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT 
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT 
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA 
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC 
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA 
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542271.1
CDS	378..4610
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543969.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543969.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT 
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA 
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT 
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT 
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA 
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC 
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA 
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543969.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542271.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu61260
Clone ID Related Accession (Same CDS sequence)	XM_011543969.2 , XM_011543969.3
Accession Version	XM_011543969.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT 
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA 
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT 
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT 
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA 
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC 
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA 
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542271.1
CDS	371..4603
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543969.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543969.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCATGT 
AAACCTCATC ATCCAAATAC TGAACCTGTA TTAGGCCTCA GTCAAACACC AATTTCACAG 
CAATCCTTGC CACTACACAT GATTCCTTCT AGCCAAGTAG ATGACCTGTC CAGTCCTGCC 
AAGAGGAAAA GAACATCTAG TCCAACAAAG AATACTTCTG ACAATTGGAG TGGTGGACAT 
GCTGTGTCAC ATCCTCCAGT ACAGCAACAA GCTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGATGA GACCAACAGG AGTTGCACAG GTACGATCTA CTGGAATTCC TAATGGGCCA 
ACAGCTGACT CATCACTGCC TACAAACTCA GTCTCTGGCC AGCAGCCACA GCTTGCTCTG 
ACCAGAGTGC CTAGCGTCTC TCAGCCTGGA GTCCGTCCTG CCTGCCCTGG GCAGCCTTTG 
GCCAATGGAC CCTTTTCTGC AGGCCATGTT CCCTGTAGCA CATCAAGAAC GCTGGGAAGT 
ACAGACACTA TTTTGATAGG CAATAATCAT ATAACAGGAA GTGGAAGTAA TGGAAACGTG 
CCTTACCTGC AGCGAAACGC ACTCACTCTA CCTCATAACC GCACAAACCT GACCAGCAGC 
GCAGAGGAGC CGTGGAAAAA CCAACTATCT AACTCCACTC AGGGGCTTCA CAAAGGTCAG 
AGTTCACATT CGGCAGGTCC TAATGGTGAA CGACCTCTCT CTTCCACTGG GCCTTCCCAG 
CATCTCCAGG CAGCTGGCTC TGGTATTCAG AATCAGAACG GACATCCCAC CCTGCCTAGC 
AATTCAGTAA CACAGGGGGC TGCTCTCAAT CACCTCTCCT CTCACACTGC TACCTCAGGT 
GGACAACAAG GCATTACCTT AACCAAAGAG AGCAAGCCTT CAGGAAACAT ATTGACGGTG 
CCTGAAACAA GCAGGCACAC TGGAGAGACA CCTAACAGCA CTGCCAGTGT CGAGGGACTT 
CCTAATCATG TCCATCAGAT GACGGCAGAT GCTGTTTGCA GTCCTAGCCA TGGAGATTCT 
AAGTCACCAG GTTTACTAAG TTCAGACAAT CCTCAGCTCT CTGCCTTGTT GATGGGAAAA 
GCCAATAACA ATGTGGGTAC TGGAACCTGT GACAAAGTCA ATAACATCCA CCCAGCTGTT 
CATACAAAGA CTGATAACTC TGTTGCCTCT TCACCATCTT CAGCCATTTC AACAGCAACA 
CCTTCTCCAA AATCCACTGA GCAGACAACC ACAAACAGTG TTACCAGCCT TAACAGCCCT 
CACAGTGGGC TACACACAAT TAATGGAGAA GGGATGGAAG AATCTCAGAG CCCCATGAAA 
ACAGATCTGC TTCTGGTTAA CCACAAACCT AGTCCACAGA TCATACCATC AATGTCTGTG 
TCCATATACC CCAGCTCAGC AGAAGTTCTG AAGGCATGCA GGAATCTAGG TAAAAATGGC 
TTATCTAACA GTAGCATTTT GTTGGATAAA TGTCCACCTC CAAGACCACC ATCTTCACCA 
TACCCTCCCT TGCCAAAGGA CAAGTTGAAT CCACCTACAC CTAGTATTTA CTTGGAAAAT 
AAACGTGATG CTTTCTTTCC TCCATTACAT CAATTTTGTA CAAATCCGAA CAACCCTGTT 
ACAGTAATAC GTGGCCTTGC TGGAGCTCTT AAGTTAGACC TGGGACTTTT CTCTACTAAA 
ACTTTGGTGG AAGCTAACAA TGAACATATG GTAGAAGTGA GGACACAGTT GTTGCAGCCA 
GCAGATGAAA ACTGGGATCC CACTGGAACA AAGAAAATCT GGCATTGTGA AAGTAATAGA 
TCTCATACTA CAATTGCTAA ATATGCACAG TACCAGGCCT CCTCATTCCA GGAATCATTG 
AGAGAAGAAA ATGAAAAAAG AAGTCATCAT AAAGACCACT CAGATAGTGA ATCTACATCG 
TCAGATAATT CTGGGAGGAG GAGGAAAGGA CCCTTTAAAA CCATAAAGTT TGGGACCAAT 
ATTGACCTAT CTGATGACAA AAAGTGGAAG TTGCAGCTAC ATGAGCTGAC TAAACTTCCT 
GCTTTTGTGC GTGTCGTATC AGCAGGAAAT CTTCTAAGCC ATGTTGGTCA TACCATATTG 
GGCATGAACA CAGTTCAACT ATACATGAAA GTTCCAGGGA GCAGAACACC AGGTCATCAG 
GAAAATAACA ACTTCTGTTC AGTTAACATA AATATTGGCC CAGGTGACTG TGAATGGTTT 
GTTGTTCCTG AAGGTTACTG GGGTGTTCTG AATGACTTCT GTGAAAAAAA TAATTTGAAT 
TTCCTAATGG GTTCTTGGTG GCCCAATCTT GAAGATCTTT ATGAAGCAAA TGTTCCAGTG 
TATAGGTTTA TTCAGCGACC TGGAGATTTG GTCTGGATAA ATGCAGGCAC TGTTCATTGG 
GTTCAGGCTA TTGGCTGGTG CAACAACATT GCTTGGAATG TTGGTCCACT TACAGCCTGC 
CAGTATAAAT TGGCAGTGGA ACGGTACGAA TGGAACAAAT TGCAAAGTGT GAAGTCAATA 
GTACCCATGG TTCATCTTTC CTGGAATATG GCACGAAATA TCAAGGTCTC AGATCCAAAG 
CTTTTTGAAA TGATTAAGAT ATTTTCATTG TCTCCGAATT TTAGAGCTGA AAAGTGCCTT 
AGAGATCATC TAGTTCAACC TCTCCGTTCA AATGGAGAAC CTGAGCCACT AAGATTCACA 
GGAGAGTATT GTCTTCTAAG AACTCTGAAG CAATGTCAGA CATTGAGGGA AGCTCTCATT 
GCTGCAGGAA AAGAGATTAT ATGGCATGGG CGGACAAAAG AAGAACCAGC TCATTACTGT 
AGCATTTGTG AAGTGGAGGT TTTTGATCTG CTTTTTGTCA CTAATGAGAG TAATTCACGA 
AAGACCTACA TAGTACATTG CCAAGATTGT GCACGAAAAA CAAGCGGAAA CTTGGAAAAC 
TTTGTGGTGC TAGAACAGTA CAAAATGGAG GACCTGATGC AAGTCTATGA CCAATTTACA 
TTAGCTCCTC CATTACCATC CGCCTCATCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543970.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542272.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu61261
Clone ID Related Accession (Same CDS sequence)	XM_011543970.2 , XM_011543970.3
Accession Version	XM_011543970.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542272.1
CDS	378..4589
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543970.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543970.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543970.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542272.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.

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CloneID	OHu61261
Clone ID Related Accession (Same CDS sequence)	XM_011543970.2 , XM_011543970.3
Accession Version	XM_011543970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542272.1
CDS	371..4582
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543970.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543970.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGGCTCAG 
TTGTGTAACC TTCCACAAGG TAGTCTACAG AATAAAACTA AATTACTTCC TAGTATTGAG 
GAGGCGTGGA GCCTACCAAT TCCCGCAGAG CTTACCTCCA GGCAGGGTGC CATGAACACA 
GCACAGCAGA ATACTTCTGA CAATTGGAGT GGTGGACATG CTGTGTCACA TCCTCCAGTA 
CAGCAACAAG CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGATGAG ACCAACAGGA 
GTTGCACAGG TACGATCTAC TGGAATTCCT AATGGGCCAA CAGCTGACTC ATCACTGCCT 
ACAAACTCAG TCTCTGGCCA GCAGCCACAG CTTGCTCTGA CCAGAGTGCC TAGCGTCTCT 
CAGCCTGGAG TCCGTCCTGC CTGCCCTGGG CAGCCTTTGG CCAATGGACC CTTTTCTGCA 
GGCCATGTTC CCTGTAGCAC ATCAAGAACG CTGGGAAGTA CAGACACTAT TTTGATAGGC 
AATAATCATA TAACAGGAAG TGGAAGTAAT GGAAACGTGC CTTACCTGCA GCGAAACGCA 
CTCACTCTAC CTCATAACCG CACAAACCTG ACCAGCAGCG CAGAGGAGCC GTGGAAAAAC 
CAACTATCTA ACTCCACTCA GGGGCTTCAC AAAGGTCAGA GTTCACATTC GGCAGGTCCT 
AATGGTGAAC GACCTCTCTC TTCCACTGGG CCTTCCCAGC ATCTCCAGGC AGCTGGCTCT 
GGTATTCAGA ATCAGAACGG ACATCCCACC CTGCCTAGCA ATTCAGTAAC ACAGGGGGCT 
GCTCTCAATC ACCTCTCCTC TCACACTGCT ACCTCAGGTG GACAACAAGG CATTACCTTA 
ACCAAAGAGA GCAAGCCTTC AGGAAACATA TTGACGGTGC CTGAAACAAG CAGGCACACT 
GGAGAGACAC CTAACAGCAC TGCCAGTGTC GAGGGACTTC CTAATCATGT CCATCAGATG 
ACGGCAGATG CTGTTTGCAG TCCTAGCCAT GGAGATTCTA AGTCACCAGG TTTACTAAGT 
TCAGACAATC CTCAGCTCTC TGCCTTGTTG ATGGGAAAAG CCAATAACAA TGTGGGTACT 
GGAACCTGTG ACAAAGTCAA TAACATCCAC CCAGCTGTTC ATACAAAGAC TGATAACTCT 
GTTGCCTCTT CACCATCTTC AGCCATTTCA ACAGCAACAC CTTCTCCAAA ATCCACTGAG 
CAGACAACCA CAAACAGTGT TACCAGCCTT AACAGCCCTC ACAGTGGGCT ACACACAATT 
AATGGAGAAG GGATGGAAGA ATCTCAGAGC CCCATGAAAA CAGATCTGCT TCTGGTTAAC 
CACAAACCTA GTCCACAGAT CATACCATCA ATGTCTGTGT CCATATACCC CAGCTCAGCA 
GAAGTTCTGA AGGCATGCAG GAATCTAGGT AAAAATGGCT TATCTAACAG TAGCATTTTG 
TTGGATAAAT GTCCACCTCC AAGACCACCA TCTTCACCAT ACCCTCCCTT GCCAAAGGAC 
AAGTTGAATC CACCTACACC TAGTATTTAC TTGGAAAATA AACGTGATGC TTTCTTTCCT 
CCATTACATC AATTTTGTAC AAATCCGAAC AACCCTGTTA CAGTAATACG TGGCCTTGCT 
GGAGCTCTTA AGTTAGACCT GGGACTTTTC TCTACTAAAA CTTTGGTGGA AGCTAACAAT 
GAACATATGG TAGAAGTGAG GACACAGTTG TTGCAGCCAG CAGATGAAAA CTGGGATCCC 
ACTGGAACAA AGAAAATCTG GCATTGTGAA AGTAATAGAT CTCATACTAC AATTGCTAAA 
TATGCACAGT ACCAGGCCTC CTCATTCCAG GAATCATTGA GAGAAGAAAA TGAAAAAAGA 
AGTCATCATA AAGACCACTC AGATAGTGAA TCTACATCGT CAGATAATTC TGGGAGGAGG 
AGGAAAGGAC CCTTTAAAAC CATAAAGTTT GGGACCAATA TTGACCTATC TGATGACAAA 
AAGTGGAAGT TGCAGCTACA TGAGCTGACT AAACTTCCTG CTTTTGTGCG TGTCGTATCA 
GCAGGAAATC TTCTAAGCCA TGTTGGTCAT ACCATATTGG GCATGAACAC AGTTCAACTA 
TACATGAAAG TTCCAGGGAG CAGAACACCA GGTCATCAGG AAAATAACAA CTTCTGTTCA 
GTTAACATAA ATATTGGCCC AGGTGACTGT GAATGGTTTG TTGTTCCTGA AGGTTACTGG 
GGTGTTCTGA ATGACTTCTG TGAAAAAAAT AATTTGAATT TCCTAATGGG TTCTTGGTGG 
CCCAATCTTG AAGATCTTTA TGAAGCAAAT GTTCCAGTGT ATAGGTTTAT TCAGCGACCT 
GGAGATTTGG TCTGGATAAA TGCAGGCACT GTTCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CTTGGAATGT TGGTCCACTT ACAGCCTGCC AGTATAAATT GGCAGTGGAA 
CGGTACGAAT GGAACAAATT GCAAAGTGTG AAGTCAATAG TACCCATGGT TCATCTTTCC 
TGGAATATGG CACGAAATAT CAAGGTCTCA GATCCAAAGC TTTTTGAAAT GATTAAGATA 
TTTTCATTGT CTCCGAATTT TAGAGCTGAA AAGTGCCTTA GAGATCATCT AGTTCAACCT 
CTCCGTTCAA ATGGAGAACC TGAGCCACTA AGATTCACAG GAGAGTATTG TCTTCTAAGA 
ACTCTGAAGC AATGTCAGAC ATTGAGGGAA GCTCTCATTG CTGCAGGAAA AGAGATTATA 
TGGCATGGGC GGACAAAAGA AGAACCAGCT CATTACTGTA GCATTTGTGA AGTGGAGGTT 
TTTGATCTGC TTTTTGTCAC TAATGAGAGT AATTCACGAA AGACCTACAT AGTACATTGC 
CAAGATTGTG CACGAAAAAC AAGCGGAAAC TTGGAAAACT TTGTGGTGCT AGAACAGTAC 
AAAATGGAGG ACCTGATGCA AGTCTATGAC CAATTTACAT TAGCTCCTCC ATTACCATCC 
GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543971.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542273.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X18, mRNA.

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CloneID	OHu61262
Clone ID Related Accession (Same CDS sequence)	XM_011543971.2
Accession Version	XM_011543971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542273.1
CDS	378..4559
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPSSQVDDLSSPAKRKRTSSPTKNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543971.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543971.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GGCATGTAAA 
CCTCATCATC CAAATACTGA ACCTGTATTA GGCCTCAGTC AAACACCAAT TTCACAGCAA 
TCCTTGCCAC TACACATGAT TCCTTCTAGC CAAGTAGATG ACCTGTCCAG TCCTGCCAAG 
AGGAAAAGAA CATCTAGTCC AACAAAGAAT ACTTCTGACA ATTGGAGTGG TGGACATGCT 
GTGTCACATC CTCCAGTACA GCAACAAGCT CATTCATGGT GTTTGACACC ACAGAAATTA 
CAGATGAGAC CAACAGGAGT TGCACAGGTA CGATCTACTG GAATTCCTAA TGGGCCAACA 
GCTGACTCAT CACTGCCTAC AAACTCAGTC TCTGGCCAGC AGCCACAGCT TGCTCTGACC 
AGAGTGCCTA GCGTCTCTCA GCCTGGAGTC CGTCCTGCCT GCCCTGGGCA GCCTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACGCT GGGAAGTACA 
GACACTATTT TGATAGGCAA TAATCATATA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACT CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCGCA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCAGAGT 
TCACATTCGG CAGGTCCTAA TGGTGAACGA CCTCTCTCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAACGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACATATT GACGGTGCCT 
GAAACAAGCA GGCACACTGG AGAGACACCT AACAGCACTG CCAGTGTCGA GGGACTTCCT 
AATCATGTCC ATCAGATGAC GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCC 
AATAACAATG TGGGTACTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AGCTGTTCAT 
ACAAAGACTG ATAACTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCAAC AGCAACACCT 
TCTCCAAAAT CCACTGAGCA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTAC ACACAATTAA TGGAGAAGGG ATGGAAGAAT CTCAGAGCCC CATGAAAACA 
GATCTGCTTC TGGTTAACCA CAAACCTAGT CCACAGATCA TACCATCAAT GTCTGTGTCC 
ATATACCCCA GCTCAGCAGA AGTTCTGAAG GCATGCAGGA ATCTAGGTAA AAATGGCTTA 
TCTAACAGTA GCATTTTGTT GGATAAATGT CCACCTCCAA GACCACCATC TTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTACTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ATCCGAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAG TTAGACCTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT GCAGCCAGCA 
GATGAAAACT GGGATCCCAC TGGAACAAAG AAAATCTGGC ATTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCACAGTAC CAGGCCTCCT CATTCCAGGA ATCATTGAGA 
GAAGAAAATG AAAAAAGAAG TCATCATAAA GACCACTCAG ATAGTGAATC TACATCGTCA 
GATAATTCTG GGAGGAGGAG GAAAGGACCC TTTAAAACCA TAAAGTTTGG GACCAATATT 
GACCTATCTG ATGACAAAAA GTGGAAGTTG CAGCTACATG AGCTGACTAA ACTTCCTGCT 
TTTGTGCGTG TCGTATCAGC AGGAAATCTT CTAAGCCATG TTGGTCATAC CATATTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGGAGCA GAACACCAGG TCATCAGGAA 
AATAACAACT TCTGTTCAGT TAACATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GTTACTGGGG TGTTCTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
CTAATGGGTT CTTGGTGGCC CAATCTTGAA GATCTTTATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATTTGGTC TGGATAAATG CAGGCACTGT TCATTGGGTT 
CAGGCTATTG GCTGGTGCAA CAACATTGCT TGGAATGTTG GTCCACTTAC AGCCTGCCAG 
TATAAATTGG CAGTGGAACG GTACGAATGG AACAAATTGC AAAGTGTGAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTCTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTAAGA ACTCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTCATTG CTGCAGGAAA AGAGATTATA TGGCATGGGC GGACAAAAGA AGAACCAGCT 
CATTACTGTA GCATTTGTGA AGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCACGAA AGACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGCGGAAAC 
TTGGAAAACT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ACCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542274.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu61263
Clone ID Related Accession (Same CDS sequence)	XM_011543972.2 , XM_011543972.3
Accession Version	XM_011543972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG 
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA 
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542274.1
CDS	378..4556
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543972.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG 
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA 
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543972.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542274.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.

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CloneID	OHu61263
Clone ID Related Accession (Same CDS sequence)	XM_011543972.2 , XM_011543972.3
Accession Version	XM_011543972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG 
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA 
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542274.1
CDS	371..4549
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKIFSLSPNFRAEKCLRDHLVQPLRSNGEPEPLRFTGEYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543972.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543972.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGAATGCT GCCTTTTTAT ATGGTCTTGG TTTGGTCTAC 
TTCCATTATA ATGCATTTCA GTGGGCAATT AAAGCATTTC AGGAGGTGCT TTATGTTGAT 
CCCAGCTTTT GTCGAGCCAA GGAAATTCAT TTACGACTTG GGCTTATGTT CAAAGTGAAC 
ACAGACTATG AGTCTAGTTT AAAGCATTTT CAGTTAGCTT TGGTTGACTG TAATCCCTGC 
ACTTTGTCCA ATGCTGAAAT TCAATTTCAC ATTGCCCACT TATATGAAAC CCAGAGGAAA 
TATCATTCTG CAAAAGAAGC TTATGAACAA CTTTTGCAGA CAGAGAATCT TTCTGCACAA 
GTAAAAGCAA CTGTCTTACA ACAGTTAGGT TGGATGCATC ACACTGTAGA TCTCCTGGGA 
GATAAAGCCA CCAAGGAAAG CTATGCTATT CAGTATCTCC AAAAGTCCTT GGAAGCAGAT 
CCTAATTCTG GCCAGTCCTG GTATTTCCTC GGAAGGTGCT ATTCAAGTAT TGGGAAAGTT 
CAGGATGCCT TTATATCTTA CAGGCAGTCT ATTGATAAAT CAGAAGCAAG TGCAGATACA 
TGGTGTTCAA TAGGTGTGCT ATATCAGCAG CAAAATCAGC CCATGGATGC TTTACAGGCC 
TATATTTGTG CTGTACAATT GGACCATGGC CATGCTGCAG CCTGGATGGA CCTAGGCACT 
CTCTATGAAT CCTGCAACCA GCCTCAGGAT GCCATTAAAT GCTACTTAAA TGCAACTAGA 
AGCAAAAGTT GTAGTAATAC CTCTGCACTT GCAGCACGAA TTAAGTATTT ACAGAATACT 
TCTGACAATT GGAGTGGTGG ACATGCTGTG TCACATCCTC CAGTACAGCA ACAAGCTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CATTTGGAAC AGCTCCGCGC AAATAGAAAT 
AATTTAAATC CAGCACAGAA ACTGATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAACAACACC AAATGAGACC AACAGGAGTT GCACAGGTAC GATCTACTGG AATTCCTAAT 
GGGCCAACAG CTGACTCATC ACTGCCTACA AACTCAGTCT CTGGCCAGCA GCCACAGCTT 
GCTCTGACCA GAGTGCCTAG CGTCTCTCAG CCTGGAGTCC GTCCTGCCTG CCCTGGGCAG 
CCTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACGCTG 
GGAAGTACAG ACACTATTTT GATAGGCAAT AATCATATAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACTC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCGCAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCAGAGTT CACATTCGGC AGGTCCTAAT GGTGAACGAC CTCTCTCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAACGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACATATTG 
ACGGTGCCTG AAACAAGCAG GCACACTGGA GAGACACCTA ACAGCACTGC CAGTGTCGAG 
GGACTTCCTA ATCATGTCCA TCAGATGACG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCCA ATAACAATGT GGGTACTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
GCTGTTCATA CAAAGACTGA TAACTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCAACA 
GCAACACCTT CTCCAAAATC CACTGAGCAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTACA CACAATTAAT GGAGAAGGGA TGGAAGAATC TCAGAGCCCC 
ATGAAAACAG ATCTGCTTCT GGTTAACCAC AAACCTAGTC CACAGATCAT ACCATCAATG 
TCTGTGTCCA TATACCCCAG CTCAGCAGAA GTTCTGAAGG CATGCAGGAA TCTAGGTAAA 
AATGGCTTAT CTAACAGTAG CATTTTGTTG GATAAATGTC CACCTCCAAG ACCACCATCT 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTACTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA TCCGAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAGT TAGACCTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTG 
CAGCCAGCAG ATGAAAACTG GGATCCCACT GGAACAAAGA AAATCTGGCA TTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCACAGTACC AGGCCTCCTC ATTCCAGGAA 
TCATTGAGAG AAGAAAATGA AAAAAGAAGT CATCATAAAG ACCACTCAGA TAGTGAATCT 
ACATCGTCAG ATAATTCTGG GAGGAGGAGG AAAGGACCCT TTAAAACCAT AAAGTTTGGG 
ACCAATATTG ACCTATCTGA TGACAAAAAG TGGAAGTTGC AGCTACATGA GCTGACTAAA 
CTTCCTGCTT TTGTGCGTGT CGTATCAGCA GGAAATCTTC TAAGCCATGT TGGTCATACC 
ATATTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGGAGCAG AACACCAGGT 
CATCAGGAAA ATAACAACTT CTGTTCAGTT AACATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG TTACTGGGGT GTTCTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCC TAATGGGTTC TTGGTGGCCC AATCTTGAAG ATCTTTATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATTTGGTCT GGATAAATGC AGGCACTGTT 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGCCAGT ATAAATTGGC AGTGGAACGG TACGAATGGA ACAAATTGCA AAGTGTGAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTCTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGATATTT TCATTGTCTC CGAATTTTAG AGCTGAAAAG 
TGCCTTAGAG ATCATCTAGT TCAACCTCTC CGTTCAAATG GAGAACCTGA GCCACTAAGA 
TTCACAGGAG AGTATTGTCT TCTAAGAACT CTGAAGCAAT GTCAGACATT GAGGGAAGCT 
CTCATTGCTG CAGGAAAAGA GATTATATGG CATGGGCGGA CAAAAGAAGA ACCAGCTCAT 
TACTGTAGCA TTTGTGAAGT GGAGGTTTTT GATCTGCTTT TTGTCACTAA TGAGAGTAAT 
TCACGAAAGA CCTACATAGT ACATTGCCAA GATTGTGCAC GAAAAACAAG CGGAAACTTG 
GAAAACTTTG TGGTGCTAGA ACAGTACAAA ATGGAGGACC TGATGCAAGT CTATGACCAA 
TTTACATTAG CTCCTCCATT ACCATCCGCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM6A ( XM_011543973.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542275.1 Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X20, mRNA.

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CloneID	OHu61264
Clone ID Related Accession (Same CDS sequence)	XM_011543973.2
Accession Version	XM_011543973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysine-specific demethylase 6A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT 
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA 
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT 
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA 
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG 
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT 
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT 
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA 
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC 
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC 
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT 
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC 
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT 
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA 
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG 
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA 
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT 
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT 
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA 
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC 
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG 
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT 
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT 
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA 
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA 
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT 
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT 
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG 
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT 
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA 
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA 
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC 
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT 
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA 
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT 
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG 
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG 
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA 
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT 
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC 
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA 
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA 
AAGCTTTTTG AAATGATTAA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG 
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA 
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT 
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC 
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC 
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542275.1
CDS	378..4505
Translation	MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKMRNPWIQRAHCIPLDQYTTNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKSCSNTSALAARIKYLQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLPTNSVSGQQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTSRTLGSTDTILIGNNHITGSGSNGNVPYLQRNALTLPHNRTNLTSSAEEPWKNQLSNSTQGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPMKTDLLLVNHKPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_011543973.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lysine demethylase 6A (KDM6A), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543973.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCTG CCGCCGCCGC TTTCGGTGAT 
GAGGAAAAGA AAATGGCGGC GGGAAAAGCG AGCGGCGAGA GCGAGGAGGC GTCCCCCAGC 
CTGACAGCCG AGGAGAGGGA GGCGCTCGGC GGACTGGACA GCCGCCTCTT TGGGTTCGTG 
AGATTTCATG AAGATGGCGC CAGGACGAAG GCCCTACTGG GCAAGGCTGT TCGCTGCTAT 
GAATCTCTAA TCTTAAAAGC TGAAGGAAAA GTGGAGTCTG ATTTCTTTTG TCAATTAGGT 
CACTTCAACC TCTTATTGGA AGATTATCCA AAAGCATTAT CTGCATACCA GAGGTACTAC 
AGTTTACAGT CTGACTACTG GAAGATGCGG AACCCATGGA TACAAAGGGC TCACTGTATT 
CCATTGGATC AATATACCAC AAATGCTGCC TTTTTATATG GTCTTGGTTT GGTCTACTTC 
CATTATAATG CATTTCAGTG GGCAATTAAA GCATTTCAGG AGGTGCTTTA TGTTGATCCC 
AGCTTTTGTC GAGCCAAGGA AATTCATTTA CGACTTGGGC TTATGTTCAA AGTGAACACA 
GACTATGAGT CTAGTTTAAA GCATTTTCAG TTAGCTTTGG TTGACTGTAA TCCCTGCACT 
TTGTCCAATG CTGAAATTCA ATTTCACATT GCCCACTTAT ATGAAACCCA GAGGAAATAT 
CATTCTGCAA AAGAAGCTTA TGAACAACTT TTGCAGACAG AGAATCTTTC TGCACAAGTA 
AAAGCAACTG TCTTACAACA GTTAGGTTGG ATGCATCACA CTGTAGATCT CCTGGGAGAT 
AAAGCCACCA AGGAAAGCTA TGCTATTCAG TATCTCCAAA AGTCCTTGGA AGCAGATCCT 
AATTCTGGCC AGTCCTGGTA TTTCCTCGGA AGGTGCTATT CAAGTATTGG GAAAGTTCAG 
GATGCCTTTA TATCTTACAG GCAGTCTATT GATAAATCAG AAGCAAGTGC AGATACATGG 
TGTTCAATAG GTGTGCTATA TCAGCAGCAA AATCAGCCCA TGGATGCTTT ACAGGCCTAT 
ATTTGTGCTG TACAATTGGA CCATGGCCAT GCTGCAGCCT GGATGGACCT AGGCACTCTC 
TATGAATCCT GCAACCAGCC TCAGGATGCC ATTAAATGCT ACTTAAATGC AACTAGAAGC 
AAAAGTTGTA GTAATACCTC TGCACTTGCA GCACGAATTA AGTATTTACA GAATACTTCT 
GACAATTGGA GTGGTGGACA TGCTGTGTCA CATCCTCCAG TACAGCAACA AGCTCATTCA 
TGGTGTTTGA CACCACAGAA ATTACAGCAT TTGGAACAGC TCCGCGCAAA TAGAAATAAT 
TTAAATCCAG CACAGAAACT GATGCTGGAA CAGCTGGAAA GTCAGTTTGT CTTAATGCAA 
CAACACCAAA TGAGACCAAC AGGAGTTGCA CAGGTACGAT CTACTGGAAT TCCTAATGGG 
CCAACAGCTG ACTCATCACT GCCTACAAAC TCAGTCTCTG GCCAGCAGCC ACAGCTTGCT 
CTGACCAGAG TGCCTAGCGT CTCTCAGCCT GGAGTCCGTC CTGCCTGCCC TGGGCAGCCT 
TTGGCCAATG GACCCTTTTC TGCAGGCCAT GTTCCCTGTA GCACATCAAG AACGCTGGGA 
AGTACAGACA CTATTTTGAT AGGCAATAAT CATATAACAG GAAGTGGAAG TAATGGAAAC 
GTGCCTTACC TGCAGCGAAA CGCACTCACT CTACCTCATA ACCGCACAAA CCTGACCAGC 
AGCGCAGAGG AGCCGTGGAA AAACCAACTA TCTAACTCCA CTCAGGGGCT TCACAAAGGT 
CAGAGTTCAC ATTCGGCAGG TCCTAATGGT GAACGACCTC TCTCTTCCAC TGGGCCTTCC 
CAGCATCTCC AGGCAGCTGG CTCTGGTATT CAGAATCAGA ACGGACATCC CACCCTGCCT 
AGCAATTCAG TAACACAGGG GGCTGCTCTC AATCACCTCT CCTCTCACAC TGCTACCTCA 
GGTGGACAAC AAGGCATTAC CTTAACCAAA GAGAGCAAGC CTTCAGGAAA CATATTGACG 
GTGCCTGAAA CAAGCAGGCA CACTGGAGAG ACACCTAACA GCACTGCCAG TGTCGAGGGA 
CTTCCTAATC ATGTCCATCA GATGACGGCA GATGCTGTTT GCAGTCCTAG CCATGGAGAT 
TCTAAGTCAC CAGGTTTACT AAGTTCAGAC AATCCTCAGC TCTCTGCCTT GTTGATGGGA 
AAAGCCAATA ACAATGTGGG TACTGGAACC TGTGACAAAG TCAATAACAT CCACCCAGCT 
GTTCATACAA AGACTGATAA CTCTGTTGCC TCTTCACCAT CTTCAGCCAT TTCAACAGCA 
ACACCTTCTC CAAAATCCAC TGAGCAGACA ACCACAAACA GTGTTACCAG CCTTAACAGC 
CCTCACAGTG GGCTACACAC AATTAATGGA GAAGGGATGG AAGAATCTCA GAGCCCCATG 
AAAACAGATC TGCTTCTGGT TAACCACAAA CCTAGTCCAC AGATCATACC ATCAATGTCT 
GTGTCCATAT ACCCCAGCTC AGCAGAAGTT CTGAAGGCAT GCAGGAATCT AGGTAAAAAT 
GGCTTATCTA ACAGTAGCAT TTTGTTGGAT AAATGTCCAC CTCCAAGACC ACCATCTTCA 
CCATACCCTC CCTTGCCAAA GGACAAGTTG AATCCACCTA CACCTAGTAT TTACTTGGAA 
AATAAACGTG ATGCTTTCTT TCCTCCATTA CATCAATTTT GTACAAATCC GAACAACCCT 
GTTACAGTAA TACGTGGCCT TGCTGGAGCT CTTAAGTTAG ACCTGGGACT TTTCTCTACT 
AAAACTTTGG TGGAAGCTAA CAATGAACAT ATGGTAGAAG TGAGGACACA GTTGTTGCAG 
CCAGCAGATG AAAACTGGGA TCCCACTGGA ACAAAGAAAA TCTGGCATTG TGAAAGTAAT 
AGATCTCATA CTACAATTGC TAAATATGCA CAGTACCAGG CCTCCTCATT CCAGGAATCA 
TTGAGAGAAG AAAATGAAAA AAGAAGTCAT CATAAAGACC ACTCAGATAG TGAATCTACA 
TCGTCAGATA ATTCTGGGAG GAGGAGGAAA GGACCCTTTA AAACCATAAA GTTTGGGACC 
AATATTGACC TATCTGATGA CAAAAAGTGG AAGTTGCAGC TACATGAGCT GACTAAACTT 
CCTGCTTTTG TGCGTGTCGT ATCAGCAGGA AATCTTCTAA GCCATGTTGG TCATACCATA 
TTGGGCATGA ACACAGTTCA ACTATACATG AAAGTTCCAG GGAGCAGAAC ACCAGGTCAT 
CAGGAAAATA ACAACTTCTG TTCAGTTAAC ATAAATATTG GCCCAGGTGA CTGTGAATGG 
TTTGTTGTTC CTGAAGGTTA CTGGGGTGTT CTGAATGACT TCTGTGAAAA AAATAATTTG 
AATTTCCTAA TGGGTTCTTG GTGGCCCAAT CTTGAAGATC TTTATGAAGC AAATGTTCCA 
GTGTATAGGT TTATTCAGCG ACCTGGAGAT TTGGTCTGGA TAAATGCAGG CACTGTTCAT 
TGGGTTCAGG CTATTGGCTG GTGCAACAAC ATTGCTTGGA ATGTTGGTCC ACTTACAGCC 
TGCCAGTATA AATTGGCAGT GGAACGGTAC GAATGGAACA AATTGCAAAG TGTGAAGTCA 
ATAGTACCCA TGGTTCATCT TTCCTGGAAT ATGGCACGAA ATATCAAGGT CTCAGATCCA 
AAGCTTTTTG AAATGATTAA GTATTGTCTT CTAAGAACTC TGAAGCAATG TCAGACATTG 
AGGGAAGCTC TCATTGCTGC AGGAAAAGAG ATTATATGGC ATGGGCGGAC AAAAGAAGAA 
CCAGCTCATT ACTGTAGCAT TTGTGAAGTG GAGGTTTTTG ATCTGCTTTT TGTCACTAAT 
GAGAGTAATT CACGAAAGAC CTACATAGTA CATTGCCAAG ATTGTGCACG AAAAACAAGC 
GGAAACTTGG AAAACTTTGT GGTGCTAGAA CAGTACAAAA TGGAGGACCT GATGCAAGTC 
TATGACCAAT TTACATTAGC TCCTCCATTA CCATCCGCCT CATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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KDM6A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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