MRPL49 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL49
Entrez Gene ID	740
Full Name	mitochondrial ribosomal protein L49
Synonyms	C11orf4,L49mt,MRP-L49,NOF,NOF1
General protein information	Preferred Names mitochondrial ribosomal protein L49 Names 39S ribosomal protein L49, mitochondrial mitochondrial large ribosomal subunit protein mL49 neighbor of FAU next to FAU
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011].
Disorder MIM:	606866

mRNA and Protein(s)

mRNA	Protein	Name
NM_004927.4	NP_004918.1	39S ribosomal protein L49, mitochondrial
NM_004927.3	NP_004918.1	39S ribosomal protein L49, mitochondrial

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC558658	XP_686994.2
Homo sapiens (human)	MRPL49	NP_004918.1
Pan troglodytes (chimpanzee)	MRPL49	XP_522058.2
Mus musculus (house mouse)	Mrpl49	NP_080522.1
	MRPL49	XP_001114460.1
Canis lupus familiaris (dog)	MRPL49	XP_540868.3
Bos taurus (cattle)	MRPL49	NP_001014871.1
Rattus norvegicus (Norway rat)	Mrpl49	NP_001041348.1
Gallus gallus (chicken)	MRPL49	XP_003643553.2
Xenopus tropicalis (tropical clawed frog)	mrpl49	XP_002937533.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Properties of the C-terminal tail of human mitochondrial inner membrane protein Oxa1L and its interactions with mammalian mitochondrial ribosomes.
Haque ME, Elmore KB, Tripathy A, Koc H, Koc EC, Spremulli LL
The Journal of biological chemistry285(36)28353-62(2010 Sep)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL49 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL49 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55793	NM_004927.4 Latest version!	Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55793	NM_004927.3	Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL49 ( NM_004927.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004918.1 Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55793
Clone ID Related Accession (Same CDS sequence)	NM_004927.4 , NM_004927.3
Accession Version	NM_004927.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	39S ribosomal protein L49, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG250495.1, BC004378.1 and AP003068.3. On May 17, 2019 this sequence version replaced NM_004927.3. Transcript Variant: This variant (1) is the predominant transcript and it encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.160957.1, SRR1660805.13441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000279242.7/ ENSP00000279242.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTA CCATGTTCCG GGCTACGCTG CGGGGATGGA GAACCGGTGT CCAGCGGGGC 
TGCGGGCTAC GGCTGTTGAG CCAGACCCAG GGCCCTCCAG ATTACCCCAG GTTTGTGGAG 
TCTGTGGATG AATATCAGTT TGTGGAGCGC CTGTTACCGG CTACCAGGAT CCCAGATCCC 
CCAAAGCATG AACATTATCC TACCCCTAGT GGCTGGCAGC CTCCCAGAGA CCCCCCACCC 
AACCTGCCTT ACTTTGTACG ACGCTCTCGG ATGCACAACA TCCCCGTCTA CAAGGACATC 
ACGCATGGCA ACCGGCAGAT GACTGTGATC CGGAAAGTGG AAGGGGACAT CTGGGCCCTG 
CAGAAAGACG TGGAAGATTT TCTGAGCCCG CTGCTGGGGA AGACACCTGT CACCCAGGTC 
AATGAGGTGA CAGGTACCCT ACGGATCAAG GGCTACTTTG ACCAGGAGCT TAAAGCCTGG 
CTCTTGGAGA AAGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004918.1
CDS	27..527
Translation	MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF

Target ORF information:

RefSeq Version	NM_004927.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004927.4

ORF Insert Sequence:

ATGGCAGCTA CCATGTTCCG GGCTACGCTG CGGGGATGGA GAACCGGTGT CCAGCGGGGC 
TGCGGGCTAC GGCTGTTGAG CCAGACCCAG GGCCCTCCAG ATTACCCCAG GTTTGTGGAG 
TCTGTGGATG AATATCAGTT TGTGGAGCGC CTGTTACCGG CTACCAGGAT CCCAGATCCC 
CCAAAGCATG AACATTATCC TACCCCTAGT GGCTGGCAGC CTCCCAGAGA CCCCCCACCC 
AACCTGCCTT ACTTTGTACG ACGCTCTCGG ATGCACAACA TCCCCGTCTA CAAGGACATC 
ACGCATGGCA ACCGGCAGAT GACTGTGATC CGGAAAGTGG AAGGGGACAT CTGGGCCCTG 
CAGAAAGACG TGGAAGATTT TCTGAGCCCG CTGCTGGGGA AGACACCTGT CACCCAGGTC 
AATGAGGTGA CAGGTACCCT ACGGATCAAG GGCTACTTTG ACCAGGAGCT TAAAGCCTGG 
CTCTTGGAGA AAGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL49 ( NM_004927.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004918.1 Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55793
Clone ID Related Accession (Same CDS sequence)	NM_004927.4 , NM_004927.3
Accession Version	NM_004927.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	39S ribosomal protein L49, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA158218.1, BG250495.1 and BC004378.1. On Nov 17, 2010 this sequence version replaced NM_004927.2. Transcript Variant: This variant (1) is the predominant transcript and it encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: BC004378.1, SRR1660809.207971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTA CCATGTTCCG GGCTACGCTG CGGGGATGGA GAACCGGTGT CCAGCGGGGC 
TGCGGGCTAC GGCTGTTGAG CCAGACCCAG GGCCCTCCAG ATTACCCCAG GTTTGTGGAG 
TCTGTGGATG AATATCAGTT TGTGGAGCGC CTGTTACCGG CTACCAGGAT CCCAGATCCC 
CCAAAGCATG AACATTATCC TACCCCTAGT GGCTGGCAGC CTCCCAGAGA CCCCCCACCC 
AACCTGCCTT ACTTTGTACG ACGCTCTCGG ATGCACAACA TCCCCGTCTA CAAGGACATC 
ACGCATGGCA ACCGGCAGAT GACTGTGATC CGGAAAGTGG AAGGGGACAT CTGGGCCCTG 
CAGAAAGACG TGGAAGATTT TCTGAGCCCG CTGCTGGGGA AGACACCTGT CACCCAGGTC 
AATGAGGTGA CAGGTACCCT ACGGATCAAG GGCTACTTTG ACCAGGAGCT TAAAGCCTGG 
CTCTTGGAGA AAGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004918.1
CDS	104..604
Translation	MAATMFRATLRGWRTGVQRGCGLRLLSQTQGPPDYPRFVESVDEYQFVERLLPATRIPDPPKHEHYPTPSGWQPPRDPPPNLPYFVRRSRMHNIPVYKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLEKGF

Target ORF information:

RefSeq Version	NM_004927.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L49 (MRPL49), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004927.3

ORF Insert Sequence:

ATGGCAGCTA CCATGTTCCG GGCTACGCTG CGGGGATGGA GAACCGGTGT CCAGCGGGGC 
TGCGGGCTAC GGCTGTTGAG CCAGACCCAG GGCCCTCCAG ATTACCCCAG GTTTGTGGAG 
TCTGTGGATG AATATCAGTT TGTGGAGCGC CTGTTACCGG CTACCAGGAT CCCAGATCCC 
CCAAAGCATG AACATTATCC TACCCCTAGT GGCTGGCAGC CTCCCAGAGA CCCCCCACCC 
AACCTGCCTT ACTTTGTACG ACGCTCTCGG ATGCACAACA TCCCCGTCTA CAAGGACATC 
ACGCATGGCA ACCGGCAGAT GACTGTGATC CGGAAAGTGG AAGGGGACAT CTGGGCCCTG 
CAGAAAGACG TGGAAGATTT TCTGAGCCCG CTGCTGGGGA AGACACCTGT CACCCAGGTC 
AATGAGGTGA CAGGTACCCT ACGGATCAAG GGCTACTTTG ACCAGGAGCT TAAAGCCTGG 
CTCTTGGAGA AAGGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Properties of the C-terminal tail of human mitochondrial inner membrane protein Oxa1L and its interactions with mammalian mitochondrial ribosomes.
The Journal of biological chemistry285(36)28353-62(2010 Sep)
Haque ME,Elmore KB,Tripathy A,Koc H,Koc EC,Spremulli LL

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MRPL49 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報