UBE2G1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2G1
Entrez Gene ID	7326
Full Name	ubiquitin conjugating enzyme E2 G1
Synonyms	E217K,UBC7,UBE2G
General protein information	Preferred Names ubiquitin conjugating enzyme E2 G1 Names ubiquitin-conjugating enzyme E2 G1 E2 ubiquitin-conjugating enzyme G1 ubiquitin carrier protein G1 ubiquitin conjugating enzyme E2G 1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7) ubiquitin-protein ligase G1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008].
Disorder MIM:	601569

mRNA and Protein(s)

mRNA	Protein	Name
NM_003342.4	NP_003333.1	ubiquitin-conjugating enzyme E2 G1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2G1	XP_001174528.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002251	XP_307933.4
Bos taurus (cattle)	UBE2G1	NP_001075927.1
Rattus norvegicus (Norway rat)	Ube2g1	NP_073181.1
Homo sapiens (human)	UBE2G1	NP_003333.1
Canis lupus familiaris (dog)	UBE2G1	XP_005620370.1
Arabidopsis thaliana (thale cress)	UBC13	NP_566884.1
Oryza sativa (rice)	Os01g0839700	NP_001044755.1
Xenopus tropicalis (tropical clawed frog)	ube2g1	NP_989051.1
	LOC721528	XP_001117702.2
Gallus gallus (chicken)	UBE2G1	NP_001074352.1
Danio rerio (zebrafish)	ube2g1a	NP_956157.1
Drosophila melanogaster (fruit fly)	CG40045	NP_001036640.1
Schizosaccharomyces pombe (fission yeast)	ubc15	NP_596465.1
Arabidopsis thaliana (thale cress)	UBC7	NP_568902.2
Mus musculus (house mouse)	Ube2g1	NP_080261.2
Oryza sativa (rice)	Os05g0460200	NP_001055755.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities.
Choi YS, Lee YJ, Lee SY, Shi L, Ha JH, Cheong HK, Cheong C, Cohen RE, Ryu KS
The Journal of biological chemistry290(4)2251-63(2015 Jan)

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Shibata E, Abbas T, Huang X, Wohlschlegel JA, Dutta A
Molecular and cellular biology31(15)3136-45(2011 Aug)

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
David Y, Ziv T, Admon A, Navon A
The Journal of biological chemistry285(12)8595-604(2010 Mar)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities
Title: Differential ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities.

study reports that UBCH8 and UBE2G1 and UBE2G2 cooperate with CRL4Cdt2 in promoting the polyubiquitylation and subsequent degradation of p21 and Cdt1, respectively
Title: Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.

In endoplasmic reticulum, involved in the degradation of type 2 iodothyronine selenodeiodinase
Title: Endoplasmic reticulum-associated degradation of the human type 2 iodothyronine deiodinase (D2) is mediated via an association between mammalian UBC7 and the carboxyl region of D2.

The following UBE2G1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2G1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu03300	NM_003342.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 G1 (UBE2G1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2G1 ( NM_003342.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003333.1 Homo sapiens ubiquitin conjugating enzyme E2 G1 (UBE2G1), mRNA.

Price & Availability↑

CloneID	OHu03300
Clone ID Related Accession (Same CDS sequence)	NM_003342.4
Accession Version	NM_003342.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	ubiquitin-conjugating enzyme E2 G1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601205.1, BG703127.1, BC002775.2, AC087742.9 and AI493944.1. On Sep 21, 2005 this sequence version replaced NM_003342.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.168454.1, SRR1660809.259419.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGGAGC TGCAGTCGGC ACTGCTACTG CGAAGACAGC TGGCAGAACT CAACAAAAAT 
CCAGTGGAAG GCTTTTCTGC AGGTTTAATA GATGACAATG ATCTCTACCG ATGGGAAGTC 
CTTATTATTG GCCCTCCAGA TACACTTTAT GAAGGTGGTG TTTTTAAGGC TCATCTTACT 
TTCCCAAAAG ATTATCCCCT CCGACCTCCT AAAATGAAAT TCATTACAGA AATCTGGCAC 
CCAAATGTTG ATAAAAATGG TGATGTGTGC ATTTCTATTC TTCATGAGCC TGGGGAAGAT 
AAGTATGGTT ATGAAAAGCC AGAGGAACGC TGGCTCCCTA TCCACACTGT GGAAACCATC 
ATGATTAGTG TCATTTCTAT GCTGGCAGAC CCTAATGGAG ACTCACCTGC TAATGTTGAT 
GCTGCGAAAG AATGGAGGGA AGATAGAAAT GGAGAATTTA AAAGAAAAGT TGCCCGCTGT 
GTAAGAAAAA GCCAAGAGAC TGCTTTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003333.1
CDS	359..871
Translation	MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQETAFE

Target ORF information:

RefSeq Version	NM_003342.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 G1 (UBE2G1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003342.4

ORF Insert Sequence:

ATGACGGAGC TGCAGTCGGC ACTGCTACTG CGAAGACAGC TGGCAGAACT CAACAAAAAT 
CCAGTGGAAG GCTTTTCTGC AGGTTTAATA GATGACAATG ATCTCTACCG ATGGGAAGTC 
CTTATTATTG GCCCTCCAGA TACACTTTAT GAAGGTGGTG TTTTTAAGGC TCATCTTACT 
TTCCCAAAAG ATTATCCCCT CCGACCTCCT AAAATGAAAT TCATTACAGA AATCTGGCAC 
CCAAATGTTG ATAAAAATGG TGATGTGTGC ATTTCTATTC TTCATGAGCC TGGGGAAGAT 
AAGTATGGTT ATGAAAAGCC AGAGGAACGC TGGCTCCCTA TCCACACTGT GGAAACCATC 
ATGATTAGTG TCATTTCTAT GCTGGCAGAC CCTAATGGAG ACTCACCTGC TAATGTTGAT 
GCTGCGAAAG AATGGAGGGA AGATAGAAAT GGAGAATTTA AAAGAAAAGT TGCCCGCTGT 
GTAAGAAAAA GCCAAGAGAC TGCTTTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential ubiquitin binding by the acidic loops of Ube2g1 and Ube2r1 enzymes distinguishes their Lys-48-ubiquitylation activities.
The Journal of biological chemistry290(4)2251-63(2015 Jan)
Choi YS,Lee YJ,Lee SY,Shi L,Ha JH,Cheong HK,Cheong C,Cohen RE,Ryu KS

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.
Molecular and cellular biology31(15)3136-45(2011 Aug)
Shibata E,Abbas T,Huang X,Wohlschlegel JA,Dutta A

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
The Journal of biological chemistry285(12)8595-604(2010 Mar)
David Y,Ziv T,Admon A,Navon A

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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UBE2G1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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