TRIO gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011514107.2	XP_011512409.1	triple functional domain protein isoform X2
XM_011514108.1	XP_011512410.1	triple functional domain protein isoform X3
XM_011514109.3	XP_011512411.1	triple functional domain protein isoform X4
NM_007118.4	NP_009049.2	triple functional domain protein
XM_011514110.3	XP_011512412.1	triple functional domain protein isoform X5
NM_007118.3	NP_009049.2	triple functional domain protein
NM_007118.2	NP_009049.2	triple functional domain protein
XM_017009802.1	XP_016865291.1	triple functional domain protein isoform X6
XM_011514110.2	XP_011512412.1	triple functional domain protein isoform X5
XM_011514109.2	XP_011512411.1	triple functional domain protein isoform X4
XM_017009801.1	XP_016865290.1	triple functional domain protein isoform X1
XM_017009803.1	XP_016865292.1	triple functional domain protein isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25435	NM_007118.4 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu61136	XM_011514107.2 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu61137	XM_011514108.1 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu61138	XM_011514109.3 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu61139	XM_011514110.3 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu96041	XM_017009801.1 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu96042	XM_017009802.1 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu96043	XM_017009803.1 Latest version!	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OHu25435	NM_007118.3	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu25435	NM_007118.2	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu61139	XM_011514110.2	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu61138	XM_011514109.2	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

TRIO ( NM_007118.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009049.2 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25435
Clone ID Related Accession (Same CDS sequence)	NM_007118.4 , NM_007118.3 , NM_007118.2
Accession Version	NM_007118.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	triple functional domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010419.6, BF058117.1, BC035585.1, U42390.1, AI478154.1, BQ691537.1, AF091395.1, BC017268.2, AK131423.1 and BQ184284.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_007118.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000344204.9/ ENSP00000339299.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009049.2
CDS	385..9678
Translation	MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	NM_007118.4
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007118.4

ORF Insert Sequence:

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( NM_007118.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009049.2 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.

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CloneID	OHu25435
Clone ID Related Accession (Same CDS sequence)	NM_007118.4 , NM_007118.3 , NM_007118.2
Accession Version	NM_007118.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	triple functional domain protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF058117.1, BC035585.1, U42390.1, AI478154.1, BQ691537.1, AF091395.1, BC017268.2, AK131423.1 and BQ184284.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2015 this sequence version replaced NM_007118.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009049.2
CDS	25..9318
Translation	MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	NM_007118.3
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007118.3

ORF Insert Sequence:

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( NM_007118.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_009049.2 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), mRNA.

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CloneID	OHu25435
Clone ID Related Accession (Same CDS sequence)	NM_007118.4 , NM_007118.3 , NM_007118.2
Accession Version	NM_007118.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	triple functional domain protein
Comment

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_009049.2

Translation

MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	NM_007118.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007118.2

ORF Insert Sequence:

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATGGCTGACC AGGGTCGCCT CCTGGACTGC 
GTGGTGCGAT GGGGAAGCCT CACTGAAGGG AAGATCAGGG CGCACCTGGG GGAGGTTCTG 
GAAGCTGTCC GGTACCTGCA CAACTGCAGG ATAGCACACC TGGACCTAAA GCCTGAGAAT 
ATCCTGGTGG ATGAGAGTTT AGCCAAGCCA ACCATCAAAC TGGCTGACTT TGGAGATGCT 
GTTCAGCTCA ACACGACCTA CTACATCCAC CAGTTACTGG GGAACCCTGA ATTCGCAGCC 
CCTGAAATCA TCCTCGGGAA CCCTGTCTCC CTGACCTCGG ATACGTGGAG TGTTGGAGTG 
CTCACATACG TACTTCTTAG TGGCGTGTCC CCCTTCCTGG ATGACAGTGT GGAAGAGACC 
TGCCTGAACA TTTGCCGCTT AGACTTTAGC TTCCCAGATG ACTACTTTAA AGGAGTGAGC 
CAGAAGGCCA AGGAGTTCGT GTGCTTCCTC CTGCAGGAGG ACCCCGCCAA GCGTCCCTCG 
GCTGCGCTGG CCCTCCAGGA GCAGTGGCTG CAGGCCGGCA ACGGCAGAAG CACGGGCGTC 
CTCGACACGT CCAGACTGAC TTCCTTCATT GAGCGGCGCA AACACCAGAA TGATGTTCGA 
CCTATCCGTA GCATTAAAAA CTTTCTGCAG AGCAGGCTTC TGCCTAGAGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514107.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512409.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

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CloneID	OHu61136
Clone ID Related Accession (Same CDS sequence)	XM_011514107.2
Accession Version	XM_011514107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514107.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGGC GCGGGCTTGG TAGGGCAGCA CGAGGCTGCC GTTCCTCTTG CCCTTCCTAC 
TTGGAGAGAA TCAGATGGCG TGCAGTGTGG ACATGGTTTC GAAAAAACGA TGAAATGAAA 
GCTATGGATG TTTTACCAAT TTTGAAGGAA AAAGTTGCAT ACCTTTCAGG TGGGAGAGAT 
AAACGTGGAG GTCCCATTTT AACGTTTCCG GCCCGCAGCA ATCATGACAG AATACGACAG 
GAGGATCTCA GGAGACTCAT TTCCTATCTA GCCTGTATTC CCAGCGAGGA GGTCTGCAAG 
CGTGGCTTCA CGGTGATCGT GGACATGCGT GGGTCCAAGT GGGACTCCAT CAAGCCCCTT 
CTGAAGATCC TGCAGGAGTC CTTCCCCTGC TGCATCCATG TGGCCCTGAT CATCAAGCCA 
GACAACTTCT GGCAGAAACA GAGGACTAAT TTTGGCAGTT CTAAATTTGA ATTTGAGACA 
AATATGGTCT CTTTAGAAGG CCTTACCAAA GTAGTTGATC CTTCTCAGCT AACTCCTGAG 
TTTGATGGCT GCCTGGAATA CAACCACGAA GAATGGATTG AAATCAGAGT TGCTTTTGAA 
GACTACATTA GCAATGCCAC CCACATGCTG TCTCGGCTGG AGGAACTTCA GGACATCCTA 
GCTAAGAAGG AGCTGCCTCA GGATTTAGAG GGGGCTCGGA ATATGATCGA GGAACATTCT 
CAGCTGAAGA AGAAGGTGAT TAAGGCCCCC ATCGAGGACC TGGATTTGGA GGGACAGAAG 
CTGCTTCAGA GGATACAGAG CAGTGAAAGC TTTCCCAAAA AGAACTCAGG CTCAGGCAAT 
GCGGACCTGC AGAACCTCTT GCCCAAGGTG TCCACCATGC TGGACCGGCT GCACTCGACA 
CGGCAGCATC TGCACCAGAT GTGGCATGTG AGGAAGCTGA AGCTGGACCA GTGCTTCCAG 
CTGAGGCTGT TTGAACAGGA TGCTGAGAAG ATGTTTGACT GGATCACACA CAACAAAGGC 
CTGTTTCTAA ACAGCTACAC AGAGATTGGG ACCAGCCACC CTCATGCCAT GGAGCTTCAG 
ACGCAGCACA ATCACTTTGC CATGAACTGT ATGAACGTGT ATGTAAATAT AAACCGCATC 
ATGTCGGTGG CCAATCGTCT GGTGGAGTCT GGCCACTATG CCTCGCAGCA GATCAGGCAG 
ATCGCGAGTC AGCTGGAGCA GGAGTGGAAG GCGTTTGCGG CAGCCCTGGA TGAGCGGAGC 
ACCTTGCTGG ACATGTCCTC CATTTTCCAC CAGAAGGCCG AAAAGTATAT GAGCAACGTG 
GATTCATGGT GTAAAGCTTG CGGTGAGGTA GACCTTCCCT CAGAGCTGCA GGACCTAGAA 
GATGCCATTC ATCACCACCA GGGAATATAT GAACATATCA CTCTTGCTTA TTCTGAGGTC 
AGCCAAGATG GGAAGTCGCT CCTTGACAAG CTCCAGCGGC CCTTGACTCC CGGCAGCTCC 
GATTCCCTGA CAGCCTCTGC CAACTACTCC AAGGCCGTGC ACCATGTCCT GGATGTCATC 
CACGAGGTGC TGCACCACCA GCGGCAGCTG GAGAACATCT GGCAACACCG CAAGGTCCGG 
CTGCATCAGA GGCTGCAGCT GTGTGTTTTC CAGCAGGACG TTCAGCAGGT GCTAGACTGG 
ATCGAGAACC ACGGAGAAGC ATTTCTGAGC AAACATACAG GTGTGGGGAA ATCTCTTCAT 
CGGGCCAGAG CATTGCAGAA ACGTCATGAA GATTTTGAAG AAGTGGCACA GAACACATAC 
ACCAATGCGG ATAAATTACT GGAAGCAGCA GAACAGCTGG CTCAGACTGG GGAATGTGAC 
CCCGAAGAGA TTTATCAGGC TGCCCATCAG CTGGAAGACC GGATTCAAGA TTTCGTTCGG 
CGTGTTGAGC AGCGAAAGAT CCTACTGGAC ATGTCAGTGT CCTTTCACAC CCATGTGAAA 
GAGCTGTGGA CGTGGCTGGA GGAGCTGCAG AAGGAGCTGC TGGACGACGT GTATGCCGAG 
TCGGTGGAGG CCGTGCAGGA CCTCATCAAG CGCTTTGGCC AGCAGCAGCA GACCACCCTG 
CAGGTGACTG TCAACGTGAT CAAGGAAGGG GAGGACCTCA TCCAGCAGCT CAGGGACTCT 
GCCATCTCCA GTAACAAGAC CCCCCACAAC AGCTCCATCA ACCACATTGA GACGGTGCTG 
CAGCAGCTGG ACGAGGCGCA GTCGCAGATG GAGGAGCTCT TCCAGGAGCG CAAGATCAAG 
CTGGAGCTCT TCCTGCAGCT GCGCATCTTC GAGAGGGACG CCATCGACAT TATCTCAGAC 
CTCGAGTCTT GGAATGATGA GCTTTCTCAG CAAATGAATG ACTTCGACAC AGAAGATCTC 
ACGATTGCAG AGCAGCGCCT CCAGCACCAT GCAGACAAAG CCTTGACCAT GAACAACTTG 
ACTTTTGACG TCATCCACCA AGGGCAAGAT CTTCTGCAGT ATGTCAATGA GGTCCAGGCC 
TCTGGTGTGG AGCTGCTGTG TGATAGAGAT GTAGACATGG CAACTCGGGT CCAGGACCTG 
CTGGAGTTTC TTCATGAAAA ACAGCAGGAA TTGGATTTAG CCGCAGAGCA GCATCGGAAA 
CACCTGGAGC AGTGCGTGCA GCTGCGCCAC CTGCAGGCAG AAGTGAAACA GGTGCTGGGT 
TGGATCCGCA ACGGAGAGTC CATGTTAAAT GCCGGACTTA TCACAGCCAG CTCGTTACAA 
GAGGCAGAGC AGCTCCAGCG AGAGCACGAG CAGTTCCAGC ATGCCATTGA GAAAACACAT 
CAGAGCGCGC TGCAGGTGCA GCAGAAGGCA GAAGCCATGC TACAGGCCAA CCACTACGAC 
ATGGACATGA TCCGGGACTG CGCCGAGAAG GTGGCGTCTC ACTGGCAACA GCTCATGCTC 
AAGATGGAAG ATCGCCTCAA GCTCGTCAAC GCCTCTGTCG CTTTCTACAA AACCTCAGAG 
CAGGTCTGCA GCGTCCTCGA GAGCCTGGAA CAGGAGTACA AGAGAGAAGA AGACTGGTGT 
GGCGGGGCGG ATAAGCTGGG CCCAAACTCT GAGACGGACC ACGTGACGCC CATGATCAGC 
AAGCACCTGG AGCAGAAGGA GGCATTCCTG AAGGCTTGCA CCCTTGCTCG GAGGAATGCA 
GACGTCTTCC TGAAATACCT GCACAGGAAC AGCGTGAACA TGCCAGGAAT GGTGACGCAC 
ATCAAAGCTC CTGAACAGCA AGTGAAAAAT ATCTTGAATG AACTCTTCCA ACGGGAGAAC 
AGGGTATTGC ATTACTGGAC CATGAGGAAG AGACGGCTGG ACCAGTGTCA GCAGTACGTG 
GTCTTTGAGA GGAGTGCCAA GCAGGCTTTG GAATGGATCC ATGACAATGG CGAGTTCTAC 
CTTTCCACAC ACACCTCCAC GGGCTCCAGT ATACAGCACA CCCAGGAGCT CCTGAAAGAG 
CACGAGGAGT TCCAGATAAC TGCAAAGCAA ACCAAAGAGA GAGTGAAGCT ATTGATACAG 
CTGGCTGATG GCTTTTGTGA AAAAGGGCAT GCCCATGCGG CAGAGATAAA AAAATGTGTT 
ACTGCTGTGG ATAAGAGGTA CAGAGATTTC TCTCTGCGGA TGGAGAAGTA CAGGACCTCT 
TTGGAAAAAG CCCTGGGGAT TTCTTCAGAT TCCAACAAAT CGAGTAAAAG TCTCCAGCTA 
GATATCATTC CAGCCAGTAT CCCTGGCTCA GAGGTGAAAC TTCGAGATGC TGCTCATGAA 
CTTAATGAAG AGAAGCGGAA ATCTGCCCGC AGGAAAGAGT TCATAATGGC TGAGCTCATT 
CAAACTGAAA AGGCTTATGT AAGAGACCTC CGGGAATGTA TGGATACGTA CCTGTGGGAA 
ATGACCAGTG GCGTGGAAGA GATTCCACCT GGCATTGTAA ACAAAGAACT CATCATCTTC 
GGAAACATGC AAGAAATCTA CGAATTTCAT AATAACATAT TCCTAAAGGA GCTGGAAAAA 
TATGAACAGT TGCCAGAGGA TGTTGGACAT TGTTTTGTTA CTTGGGCAGA CAAGTTTCAG 
ATGTATGTCA CATATTGCAA AAATAAGCCT GATTCTACTC AGCTGATATT GGAACATGCA 
GGGTCCTATT TTGACGAGAT ACAGCAGCGA CATGGATTAG CCAATTCCAT TTCTTCCTAC 
CTTATTAAAC CAGTTCAGCG AATAACGAAG TATCAGCTCC TTTTAAAAGA GCTGCTGACG 
TGCTGTGAGG AAGGAAAGGG AGAGATTAAA GATGGCCTGG AGGTGATGCT CAGCGTGCCG 
AAGCGAGCCA ATGATGCCAT GCACCTCAGC ATGCTGGAAG GGTTTGATGA AAACATTGAG 
TCTCAGGGAG AACTCATCCT ACAGGAATCC TTCCAAGTGT GGGACCCAAA AACCTTAATT 
CGAAAGGGTC GAGAACGGCA TCTCTTCCTT TTTGAAATGT CCTTAGTATT TAGTAAAGAA 
GTGAAAGATT CCAGTGGGAG AAGCAAGTAC CTTTATAAAA GCAAATTGTT TACCTCAGAG 
TTGGGTGTCA CAGAACATGT TGAAGGAGAC CCTTGCAAAT TTGCACTGTG GGTGGGGAGA 
ACACCAACTT CAGATAATAA AATTGTCCTT AAGGCTTCCA GCATAGAGAA CAAGCAGGAC 
TGGATAAAGC ATATCCGCGA AGTCATCCAG GAGCGGACGA TCCACCTGAA GGGAGCCCTG 
AAGGAGCCCA TTCACATCCC TAAGACCGCT CCCGCCACAA GACAGAAGGG AAGGAGGGAT 
GGAGAGGATC TGGACAGCCA AGGAGACGGC AGCAGCCAGC CTGATACGAT TTCCATCGCC 
TCACGGACGT CTCAGAACAC GCTGGACAGC GATAAGCTCT CTGGTGGCTG TGAGCTGACA 
GTGGTGATCC ATGACTTCAC CGCTTGCAAC AGCAACGAGC TGACCATCCG ACGGGGCCAG 
ACCGTGGAAG TTCTGGAGCG GCCGCATGAC AAGCCTGACT GGTGTCTGGT GCGGACAACT 
GACCGCTCCC CAGCGGCAGA AGGCCTGGTC CCCTGTGGTT CACTGTGCAT CGCCCACTCC 
AGAAGTAGCA TGGAAATGGA GGGCATCTTC AACCACAAAG ACTCGCTCTC CGTCTCCAGC 
AATGACGCCA GTCCACCCGC ATCCGTGGCT TCCCTCCAGC CCCACATGAT CGGGGCCCAG 
AGCTCGCCGG GCCCCAAGCG GCCGGGCAAC ACCCTGCGCA AGTGGCTCAC CAGCCCCGTG 
CGGCGGCTCA GCAGCGGCAA GGCCGACGGG CACGTGAAGA AGCTGGCGCA CAAGCACAAG 
AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC GCCGGCTCGC AGAAGGACTC CGACGACAGT 
GCGGCCACCC CGCAGGACGA GACGGTCGAG GAGAGAGGCC GGAACGAGGG CCTGAGCAGC 
GGTACTCTCT CCAAATCCTC CTCCTCGGGG ATGCAGAGCT GTGGAGAAGA GGAAGGCGAG 
GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA CCCATGGCCA TCCAGCAGCA CAGCCTCCTC 
CAGCCAGACT CACAGGATGA CAAGGCCTCT TCTCGGTTAT TAGTCCGCCC CACCAGCTCC 
GAAACACCGA GTGCAGCCGA GCTCGTCAGT GCAATTGAGG AACTCGTGAA AAGCAAGATG 
GCACTGGAGG ATCGCCCCAG CTCACTCCTT GTTGACCAGG GAGATAGTAG CAGCCCTTCC 
TTCAACCCTT CGGATAATTC CCTTCTCTCT TCCTCCTCGC CCATTGATGA GATGGAAGAA 
AGGAAATCCA GCTCTTTAAA GAGAAGACAC TACGTTTTGC AAGAACTAGT GGAGACAGAG 
CGTGACTATG TGCGGGACCT TGGCTATGTG GTTGAGGGCT ACATGGCACT TATGAAAGAA 
GATGGTGTTC CTGATGACAT GAAAGGAAAA GACAAAATTG TGTTCGGCAA CATCCATCAG 
ATTTACGACT GGCACAGAGA CTTTTTTTTA GGAGAGTTAG AGAAGTGCCT TGAAGATCCA 
GAAAAACTAG GATCCCTTTT TGTTAAACAC GAGAGAAGGT TGCACATGTA CATAGCTTAT 
TGTCAAAATA AACCAAAGTC TGAGCACATT GTCTCAGAAT ACATTGATAC CTTTTTTGAG 
GACTTAAAGC AGCGTCTTGG CCACAGGTTA CAGCTCACAG ATCTGTTGAT CAAACCAGTG 
CAGAGAATCA TGAAGTATCA GCTGTTACTG AAGGACTTCC TCAAGTATTC CAAAAAGGCC 
AGCCTGGATA CATCAGAATT AGAGAGAGCT GTGGAAGTCA TGTGCATAGT ACCCAGGCGG 
TGCAACGACA TGATGAACGT GGGGCGGCTG CAAGGATTCG ACGGGAAAAT CGTTGCCCAG 
GGTAAACTGC TCTTGCAGGA CACATTCTTG GTCACAGACC AAGATGCAGG ACTTCTGCCT 
CGCTGCAGAG AGAGGCGCAT CTTCCTCTTT GAGCAGATCG TCATATTCAG CGAACCACTT 
GATAAAAAGA AGGGCTTCTC CATGCCGGGA TTCCTGTTTA AGAACAGTAT CAAGGTGAGT 
TGCCTTTGCC TGGAGGAAAA TGTGGAAAAT GATCCCTGTA AATTTGCTCT GACATCGAGG 
ACGGGTGACG TGGTAGAGAC CTTCATTTTG CATTCATCTA GTCCAAGTGT CCGGCAAACT 
TGGATCCATG AAATCAACCA AATTTTAGAA AACCAGCGCA ATTTTTTAAA TGCCTTGACA 
TCGCCAATCG AGTACCAGAG GAACCACAGC GGGGGCGGCG GCGGCGGCGG CAGCGGGGGC 
AGCGGCGGGG GTGGGGGCAG CGGCGGCGGC GGGGCCCCCA GTGGCGGCAG CGGCCACAGT 
GGCGGCCCCA GCAGCTGCGG CGGCGCCCCC AGCACGAGCA GGAGCCGGCC CTCCCGGATC 
CCCCAGCCTG TCCGACACCA CCCCCCCGTG CTGGTCTCCT CTGCAGCCTC GAGCCAGGCA 
GAGGCAGACA AGATGTCAGG TACGTCCACC CCCGGGCCCT CCCTGCCTCC CCCTGGCGCG 
GCCCCCGAGG CCGGCCCCAG CGCGCCCAGC AGGCGGCCCC CCGGCGCGGA CGCCGAGGGG 
TCCGAGCGAG AAGCGGAGCC GATCCCCAAG ATGAAGGTGC TGGAGAGCCC CAGGAAAGGC 
GCCGCGAACG CCTCGGGGTC GAGCCCAGAC GCCCCCGCCA AGGACGCGCG CGCTAGCCTG 
GGCACCCTGC CGCTTGGGAA GCCCCGGGCC GGGGCCGCTT CGCCGCTGAA CTCGCCGCTC 
TCCAGCGCGG TCCCTTCTCT CGGCAAGGAG CCCTTCCCCC CCAGCAGCCC CCTGCAGAAG 
GGGGGCTCCT TCTGGAGCTC CATCCCCGCC TCCCCCGCCA GCCGACCCGG CTCCTTCACC 
TTCCCGGGGG ACAGCGACTC CCTCCAGCGG CAGACACCCC GCCACGCGGC CCCTGGCAAG 
GATACTGACC GCATGAGCAC GTGCTCCTCG GCCAGCGAGC AGTCCGTGCA GTCCACCCAG 
AGCAACGGGA GTGAAAGCAG CAGCAGTAGC AACATCTCCA CCATGTTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATCAAC GTCTACCAAG GAGAGGTCGT TCAAATTCTG 
GCCAGCAACC AGCAGAACAT GTTTCTGGTG TTCCGAGCCG CCACTGACCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCAGG CTTTGTCCTG GGCCACACCA GTGCAGTCAT CGTGGAGAAC 
CCGGACGGGA CTCTCAAGAA GTCAACATCT TGGCACACAG CACTCCGTTT AAGGAAAAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGT TAGAGAACGG TTATCGGAAG 
TCACGGGAAG GACTCAGCAA CAAGGTATCT GTGAAGCTTC TCAATCCCAA CTACATTTAT 
GACGTTCCCC CAGAATTCGT CATTCCATTG AGTGAGGTCA CGTGTGAGAC AGGGGAGACC 
GTTGTTCTTA GATGTCGAGT CTGTGGCCGC CCCAAAGCCT CAATTACCTG GAAGGGCCCT 
GAACACAACA CCTTGAACAA CGATGGTCAC TACAGCATCT CCTACAGTGA CCTGGGAGAG 
GCCACGCTGA AGATTGTGGG CGTGACCACG GAAGATGACG GCATCTACAC GTGCATCGCT 
GTCAATGACA TGGGTTCAGC CTCATCGTCG GCCAGCCTGA GGGTCCTAGG TCCAGGGATG 
GATGGGATCA TGGTGACCTG GAAAGACAAC TTTGACTCCT TCTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTCTC TGTCGTTAAG AAATGTGATC AGAAAGGAAC CAAGCGAGCA 
GTGGCCACTA AGTTTGTGAA CAAGAAGTTG ATGAAGCGCG ACCAGGTCAC CCATGAGCTT 
GGCATCCTGC AGAGCCTCCA GCACCCCCTG CTTGTCGGCC TCCTCGACAC CTTTGAGACC 
CCCACCAGCT ACATCCTGGT CTTAGAAATG GCTGACCAGG GTCGCCTCCT GGACTGCGTG 
GTGCGATGGG GAAGCCTCAC TGAAGGGAAG ATCAGGGCGC ACCTGGGGGA GGTTCTGGAA 
GCTGTCCGGT ACCTGCACAA CTGCAGGATA GCACACCTGG ACCTAAAGCC TGAGAATATC 
CTGGTGGATG AGAGTTTAGC CAAGCCAACC ATCAAACTGG CTGACTTTGG AGATGCTGTT 
CAGCTCAACA CGACCTACTA CATCCACCAG TTACTGGGGA ACCCTGAATT CGCAGCCCCT 
GAAATCATCC TCGGGAACCC TGTCTCCCTG ACCTCGGATA CGTGGAGTGT TGGAGTGCTC 
ACATACGTAC TTCTTAGTGG CGTGTCCCCC TTCCTGGATG ACAGTGTGGA AGAGACCTGC 
CTGAACATTT GCCGCTTAGA CTTTAGCTTC CCAGATGACT ACTTTAAAGG AGTGAGCCAG 
AAGGCCAAGG AGTTCGTGTG CTTCCTCCTG CAGGAGGACC CCGCCAAGCG TCCCTCGGCT 
GCGCTGGCCC TCCAGGAGCA GTGGCTGCAG GCCGGCAACG GCAGAAGCAC GGGCGTCCTC 
GACACGTCCA GACTGACTTC CTTCATTGAG CGGCGCAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT TCTGCAGAGC AGGCTTCTGC CTAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512409.1
CDS	663..9893
Translation	MLRRGLGRAARGCRSSCPSYLERIRWRAVWTWFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514107.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514107.2

ORF Insert Sequence:

ATGTTAAGGC GCGGGCTTGG TAGGGCAGCA CGAGGCTGCC GTTCCTCTTG CCCTTCCTAC 
TTGGAGAGAA TCAGATGGCG TGCAGTGTGG ACATGGTTTC GAAAAAACGA TGAAATGAAA 
GCTATGGATG TTTTACCAAT TTTGAAGGAA AAAGTTGCAT ACCTTTCAGG TGGGAGAGAT 
AAACGTGGAG GTCCCATTTT AACGTTTCCG GCCCGCAGCA ATCATGACAG AATACGACAG 
GAGGATCTCA GGAGACTCAT TTCCTATCTA GCCTGTATTC CCAGCGAGGA GGTCTGCAAG 
CGTGGCTTCA CGGTGATCGT GGACATGCGT GGGTCCAAGT GGGACTCCAT CAAGCCCCTT 
CTGAAGATCC TGCAGGAGTC CTTCCCCTGC TGCATCCATG TGGCCCTGAT CATCAAGCCA 
GACAACTTCT GGCAGAAACA GAGGACTAAT TTTGGCAGTT CTAAATTTGA ATTTGAGACA 
AATATGGTCT CTTTAGAAGG CCTTACCAAA GTAGTTGATC CTTCTCAGCT AACTCCTGAG 
TTTGATGGCT GCCTGGAATA CAACCACGAA GAATGGATTG AAATCAGAGT TGCTTTTGAA 
GACTACATTA GCAATGCCAC CCACATGCTG TCTCGGCTGG AGGAACTTCA GGACATCCTA 
GCTAAGAAGG AGCTGCCTCA GGATTTAGAG GGGGCTCGGA ATATGATCGA GGAACATTCT 
CAGCTGAAGA AGAAGGTGAT TAAGGCCCCC ATCGAGGACC TGGATTTGGA GGGACAGAAG 
CTGCTTCAGA GGATACAGAG CAGTGAAAGC TTTCCCAAAA AGAACTCAGG CTCAGGCAAT 
GCGGACCTGC AGAACCTCTT GCCCAAGGTG TCCACCATGC TGGACCGGCT GCACTCGACA 
CGGCAGCATC TGCACCAGAT GTGGCATGTG AGGAAGCTGA AGCTGGACCA GTGCTTCCAG 
CTGAGGCTGT TTGAACAGGA TGCTGAGAAG ATGTTTGACT GGATCACACA CAACAAAGGC 
CTGTTTCTAA ACAGCTACAC AGAGATTGGG ACCAGCCACC CTCATGCCAT GGAGCTTCAG 
ACGCAGCACA ATCACTTTGC CATGAACTGT ATGAACGTGT ATGTAAATAT AAACCGCATC 
ATGTCGGTGG CCAATCGTCT GGTGGAGTCT GGCCACTATG CCTCGCAGCA GATCAGGCAG 
ATCGCGAGTC AGCTGGAGCA GGAGTGGAAG GCGTTTGCGG CAGCCCTGGA TGAGCGGAGC 
ACCTTGCTGG ACATGTCCTC CATTTTCCAC CAGAAGGCCG AAAAGTATAT GAGCAACGTG 
GATTCATGGT GTAAAGCTTG CGGTGAGGTA GACCTTCCCT CAGAGCTGCA GGACCTAGAA 
GATGCCATTC ATCACCACCA GGGAATATAT GAACATATCA CTCTTGCTTA TTCTGAGGTC 
AGCCAAGATG GGAAGTCGCT CCTTGACAAG CTCCAGCGGC CCTTGACTCC CGGCAGCTCC 
GATTCCCTGA CAGCCTCTGC CAACTACTCC AAGGCCGTGC ACCATGTCCT GGATGTCATC 
CACGAGGTGC TGCACCACCA GCGGCAGCTG GAGAACATCT GGCAACACCG CAAGGTCCGG 
CTGCATCAGA GGCTGCAGCT GTGTGTTTTC CAGCAGGACG TTCAGCAGGT GCTAGACTGG 
ATCGAGAACC ACGGAGAAGC ATTTCTGAGC AAACATACAG GTGTGGGGAA ATCTCTTCAT 
CGGGCCAGAG CATTGCAGAA ACGTCATGAA GATTTTGAAG AAGTGGCACA GAACACATAC 
ACCAATGCGG ATAAATTACT GGAAGCAGCA GAACAGCTGG CTCAGACTGG GGAATGTGAC 
CCCGAAGAGA TTTATCAGGC TGCCCATCAG CTGGAAGACC GGATTCAAGA TTTCGTTCGG 
CGTGTTGAGC AGCGAAAGAT CCTACTGGAC ATGTCAGTGT CCTTTCACAC CCATGTGAAA 
GAGCTGTGGA CGTGGCTGGA GGAGCTGCAG AAGGAGCTGC TGGACGACGT GTATGCCGAG 
TCGGTGGAGG CCGTGCAGGA CCTCATCAAG CGCTTTGGCC AGCAGCAGCA GACCACCCTG 
CAGGTGACTG TCAACGTGAT CAAGGAAGGG GAGGACCTCA TCCAGCAGCT CAGGGACTCT 
GCCATCTCCA GTAACAAGAC CCCCCACAAC AGCTCCATCA ACCACATTGA GACGGTGCTG 
CAGCAGCTGG ACGAGGCGCA GTCGCAGATG GAGGAGCTCT TCCAGGAGCG CAAGATCAAG 
CTGGAGCTCT TCCTGCAGCT GCGCATCTTC GAGAGGGACG CCATCGACAT TATCTCAGAC 
CTCGAGTCTT GGAATGATGA GCTTTCTCAG CAAATGAATG ACTTCGACAC AGAAGATCTC 
ACGATTGCAG AGCAGCGCCT CCAGCACCAT GCAGACAAAG CCTTGACCAT GAACAACTTG 
ACTTTTGACG TCATCCACCA AGGGCAAGAT CTTCTGCAGT ATGTCAATGA GGTCCAGGCC 
TCTGGTGTGG AGCTGCTGTG TGATAGAGAT GTAGACATGG CAACTCGGGT CCAGGACCTG 
CTGGAGTTTC TTCATGAAAA ACAGCAGGAA TTGGATTTAG CCGCAGAGCA GCATCGGAAA 
CACCTGGAGC AGTGCGTGCA GCTGCGCCAC CTGCAGGCAG AAGTGAAACA GGTGCTGGGT 
TGGATCCGCA ACGGAGAGTC CATGTTAAAT GCCGGACTTA TCACAGCCAG CTCGTTACAA 
GAGGCAGAGC AGCTCCAGCG AGAGCACGAG CAGTTCCAGC ATGCCATTGA GAAAACACAT 
CAGAGCGCGC TGCAGGTGCA GCAGAAGGCA GAAGCCATGC TACAGGCCAA CCACTACGAC 
ATGGACATGA TCCGGGACTG CGCCGAGAAG GTGGCGTCTC ACTGGCAACA GCTCATGCTC 
AAGATGGAAG ATCGCCTCAA GCTCGTCAAC GCCTCTGTCG CTTTCTACAA AACCTCAGAG 
CAGGTCTGCA GCGTCCTCGA GAGCCTGGAA CAGGAGTACA AGAGAGAAGA AGACTGGTGT 
GGCGGGGCGG ATAAGCTGGG CCCAAACTCT GAGACGGACC ACGTGACGCC CATGATCAGC 
AAGCACCTGG AGCAGAAGGA GGCATTCCTG AAGGCTTGCA CCCTTGCTCG GAGGAATGCA 
GACGTCTTCC TGAAATACCT GCACAGGAAC AGCGTGAACA TGCCAGGAAT GGTGACGCAC 
ATCAAAGCTC CTGAACAGCA AGTGAAAAAT ATCTTGAATG AACTCTTCCA ACGGGAGAAC 
AGGGTATTGC ATTACTGGAC CATGAGGAAG AGACGGCTGG ACCAGTGTCA GCAGTACGTG 
GTCTTTGAGA GGAGTGCCAA GCAGGCTTTG GAATGGATCC ATGACAATGG CGAGTTCTAC 
CTTTCCACAC ACACCTCCAC GGGCTCCAGT ATACAGCACA CCCAGGAGCT CCTGAAAGAG 
CACGAGGAGT TCCAGATAAC TGCAAAGCAA ACCAAAGAGA GAGTGAAGCT ATTGATACAG 
CTGGCTGATG GCTTTTGTGA AAAAGGGCAT GCCCATGCGG CAGAGATAAA AAAATGTGTT 
ACTGCTGTGG ATAAGAGGTA CAGAGATTTC TCTCTGCGGA TGGAGAAGTA CAGGACCTCT 
TTGGAAAAAG CCCTGGGGAT TTCTTCAGAT TCCAACAAAT CGAGTAAAAG TCTCCAGCTA 
GATATCATTC CAGCCAGTAT CCCTGGCTCA GAGGTGAAAC TTCGAGATGC TGCTCATGAA 
CTTAATGAAG AGAAGCGGAA ATCTGCCCGC AGGAAAGAGT TCATAATGGC TGAGCTCATT 
CAAACTGAAA AGGCTTATGT AAGAGACCTC CGGGAATGTA TGGATACGTA CCTGTGGGAA 
ATGACCAGTG GCGTGGAAGA GATTCCACCT GGCATTGTAA ACAAAGAACT CATCATCTTC 
GGAAACATGC AAGAAATCTA CGAATTTCAT AATAACATAT TCCTAAAGGA GCTGGAAAAA 
TATGAACAGT TGCCAGAGGA TGTTGGACAT TGTTTTGTTA CTTGGGCAGA CAAGTTTCAG 
ATGTATGTCA CATATTGCAA AAATAAGCCT GATTCTACTC AGCTGATATT GGAACATGCA 
GGGTCCTATT TTGACGAGAT ACAGCAGCGA CATGGATTAG CCAATTCCAT TTCTTCCTAC 
CTTATTAAAC CAGTTCAGCG AATAACGAAG TATCAGCTCC TTTTAAAAGA GCTGCTGACG 
TGCTGTGAGG AAGGAAAGGG AGAGATTAAA GATGGCCTGG AGGTGATGCT CAGCGTGCCG 
AAGCGAGCCA ATGATGCCAT GCACCTCAGC ATGCTGGAAG GGTTTGATGA AAACATTGAG 
TCTCAGGGAG AACTCATCCT ACAGGAATCC TTCCAAGTGT GGGACCCAAA AACCTTAATT 
CGAAAGGGTC GAGAACGGCA TCTCTTCCTT TTTGAAATGT CCTTAGTATT TAGTAAAGAA 
GTGAAAGATT CCAGTGGGAG AAGCAAGTAC CTTTATAAAA GCAAATTGTT TACCTCAGAG 
TTGGGTGTCA CAGAACATGT TGAAGGAGAC CCTTGCAAAT TTGCACTGTG GGTGGGGAGA 
ACACCAACTT CAGATAATAA AATTGTCCTT AAGGCTTCCA GCATAGAGAA CAAGCAGGAC 
TGGATAAAGC ATATCCGCGA AGTCATCCAG GAGCGGACGA TCCACCTGAA GGGAGCCCTG 
AAGGAGCCCA TTCACATCCC TAAGACCGCT CCCGCCACAA GACAGAAGGG AAGGAGGGAT 
GGAGAGGATC TGGACAGCCA AGGAGACGGC AGCAGCCAGC CTGATACGAT TTCCATCGCC 
TCACGGACGT CTCAGAACAC GCTGGACAGC GATAAGCTCT CTGGTGGCTG TGAGCTGACA 
GTGGTGATCC ATGACTTCAC CGCTTGCAAC AGCAACGAGC TGACCATCCG ACGGGGCCAG 
ACCGTGGAAG TTCTGGAGCG GCCGCATGAC AAGCCTGACT GGTGTCTGGT GCGGACAACT 
GACCGCTCCC CAGCGGCAGA AGGCCTGGTC CCCTGTGGTT CACTGTGCAT CGCCCACTCC 
AGAAGTAGCA TGGAAATGGA GGGCATCTTC AACCACAAAG ACTCGCTCTC CGTCTCCAGC 
AATGACGCCA GTCCACCCGC ATCCGTGGCT TCCCTCCAGC CCCACATGAT CGGGGCCCAG 
AGCTCGCCGG GCCCCAAGCG GCCGGGCAAC ACCCTGCGCA AGTGGCTCAC CAGCCCCGTG 
CGGCGGCTCA GCAGCGGCAA GGCCGACGGG CACGTGAAGA AGCTGGCGCA CAAGCACAAG 
AAGAGCCGCG AGGTCCGCAA GAGCGCCGAC GCCGGCTCGC AGAAGGACTC CGACGACAGT 
GCGGCCACCC CGCAGGACGA GACGGTCGAG GAGAGAGGCC GGAACGAGGG CCTGAGCAGC 
GGTACTCTCT CCAAATCCTC CTCCTCGGGG ATGCAGAGCT GTGGAGAAGA GGAAGGCGAG 
GAGGGGGCCG ACGCCGTGCC CCTGCCGCCA CCCATGGCCA TCCAGCAGCA CAGCCTCCTC 
CAGCCAGACT CACAGGATGA CAAGGCCTCT TCTCGGTTAT TAGTCCGCCC CACCAGCTCC 
GAAACACCGA GTGCAGCCGA GCTCGTCAGT GCAATTGAGG AACTCGTGAA AAGCAAGATG 
GCACTGGAGG ATCGCCCCAG CTCACTCCTT GTTGACCAGG GAGATAGTAG CAGCCCTTCC 
TTCAACCCTT CGGATAATTC CCTTCTCTCT TCCTCCTCGC CCATTGATGA GATGGAAGAA 
AGGAAATCCA GCTCTTTAAA GAGAAGACAC TACGTTTTGC AAGAACTAGT GGAGACAGAG 
CGTGACTATG TGCGGGACCT TGGCTATGTG GTTGAGGGCT ACATGGCACT TATGAAAGAA 
GATGGTGTTC CTGATGACAT GAAAGGAAAA GACAAAATTG TGTTCGGCAA CATCCATCAG 
ATTTACGACT GGCACAGAGA CTTTTTTTTA GGAGAGTTAG AGAAGTGCCT TGAAGATCCA 
GAAAAACTAG GATCCCTTTT TGTTAAACAC GAGAGAAGGT TGCACATGTA CATAGCTTAT 
TGTCAAAATA AACCAAAGTC TGAGCACATT GTCTCAGAAT ACATTGATAC CTTTTTTGAG 
GACTTAAAGC AGCGTCTTGG CCACAGGTTA CAGCTCACAG ATCTGTTGAT CAAACCAGTG 
CAGAGAATCA TGAAGTATCA GCTGTTACTG AAGGACTTCC TCAAGTATTC CAAAAAGGCC 
AGCCTGGATA CATCAGAATT AGAGAGAGCT GTGGAAGTCA TGTGCATAGT ACCCAGGCGG 
TGCAACGACA TGATGAACGT GGGGCGGCTG CAAGGATTCG ACGGGAAAAT CGTTGCCCAG 
GGTAAACTGC TCTTGCAGGA CACATTCTTG GTCACAGACC AAGATGCAGG ACTTCTGCCT 
CGCTGCAGAG AGAGGCGCAT CTTCCTCTTT GAGCAGATCG TCATATTCAG CGAACCACTT 
GATAAAAAGA AGGGCTTCTC CATGCCGGGA TTCCTGTTTA AGAACAGTAT CAAGGTGAGT 
TGCCTTTGCC TGGAGGAAAA TGTGGAAAAT GATCCCTGTA AATTTGCTCT GACATCGAGG 
ACGGGTGACG TGGTAGAGAC CTTCATTTTG CATTCATCTA GTCCAAGTGT CCGGCAAACT 
TGGATCCATG AAATCAACCA AATTTTAGAA AACCAGCGCA ATTTTTTAAA TGCCTTGACA 
TCGCCAATCG AGTACCAGAG GAACCACAGC GGGGGCGGCG GCGGCGGCGG CAGCGGGGGC 
AGCGGCGGGG GTGGGGGCAG CGGCGGCGGC GGGGCCCCCA GTGGCGGCAG CGGCCACAGT 
GGCGGCCCCA GCAGCTGCGG CGGCGCCCCC AGCACGAGCA GGAGCCGGCC CTCCCGGATC 
CCCCAGCCTG TCCGACACCA CCCCCCCGTG CTGGTCTCCT CTGCAGCCTC GAGCCAGGCA 
GAGGCAGACA AGATGTCAGG TACGTCCACC CCCGGGCCCT CCCTGCCTCC CCCTGGCGCG 
GCCCCCGAGG CCGGCCCCAG CGCGCCCAGC AGGCGGCCCC CCGGCGCGGA CGCCGAGGGG 
TCCGAGCGAG AAGCGGAGCC GATCCCCAAG ATGAAGGTGC TGGAGAGCCC CAGGAAAGGC 
GCCGCGAACG CCTCGGGGTC GAGCCCAGAC GCCCCCGCCA AGGACGCGCG CGCTAGCCTG 
GGCACCCTGC CGCTTGGGAA GCCCCGGGCC GGGGCCGCTT CGCCGCTGAA CTCGCCGCTC 
TCCAGCGCGG TCCCTTCTCT CGGCAAGGAG CCCTTCCCCC CCAGCAGCCC CCTGCAGAAG 
GGGGGCTCCT TCTGGAGCTC CATCCCCGCC TCCCCCGCCA GCCGACCCGG CTCCTTCACC 
TTCCCGGGGG ACAGCGACTC CCTCCAGCGG CAGACACCCC GCCACGCGGC CCCTGGCAAG 
GATACTGACC GCATGAGCAC GTGCTCCTCG GCCAGCGAGC AGTCCGTGCA GTCCACCCAG 
AGCAACGGGA GTGAAAGCAG CAGCAGTAGC AACATCTCCA CCATGTTGGT GACACACGAT 
TACACGGCAG TGAAGGAGGA TGAGATCAAC GTCTACCAAG GAGAGGTCGT TCAAATTCTG 
GCCAGCAACC AGCAGAACAT GTTTCTGGTG TTCCGAGCCG CCACTGACCA GTGCCCCGCA 
GCTGAGGGCT GGATTCCAGG CTTTGTCCTG GGCCACACCA GTGCAGTCAT CGTGGAGAAC 
CCGGACGGGA CTCTCAAGAA GTCAACATCT TGGCACACAG CACTCCGTTT AAGGAAAAAA 
TCTGAGAAAA AAGATAAAGA CGGCAAAAGG GAAGGCAAGT TAGAGAACGG TTATCGGAAG 
TCACGGGAAG GACTCAGCAA CAAGGTATCT GTGAAGCTTC TCAATCCCAA CTACATTTAT 
GACGTTCCCC CAGAATTCGT CATTCCATTG AGTGAGGTCA CGTGTGAGAC AGGGGAGACC 
GTTGTTCTTA GATGTCGAGT CTGTGGCCGC CCCAAAGCCT CAATTACCTG GAAGGGCCCT 
GAACACAACA CCTTGAACAA CGATGGTCAC TACAGCATCT CCTACAGTGA CCTGGGAGAG 
GCCACGCTGA AGATTGTGGG CGTGACCACG GAAGATGACG GCATCTACAC GTGCATCGCT 
GTCAATGACA TGGGTTCAGC CTCATCGTCG GCCAGCCTGA GGGTCCTAGG TCCAGGGATG 
GATGGGATCA TGGTGACCTG GAAAGACAAC TTTGACTCCT TCTACAGTGA AGTGGCTGAG 
CTTGGCAGGG GCAGATTCTC TGTCGTTAAG AAATGTGATC AGAAAGGAAC CAAGCGAGCA 
GTGGCCACTA AGTTTGTGAA CAAGAAGTTG ATGAAGCGCG ACCAGGTCAC CCATGAGCTT 
GGCATCCTGC AGAGCCTCCA GCACCCCCTG CTTGTCGGCC TCCTCGACAC CTTTGAGACC 
CCCACCAGCT ACATCCTGGT CTTAGAAATG GCTGACCAGG GTCGCCTCCT GGACTGCGTG 
GTGCGATGGG GAAGCCTCAC TGAAGGGAAG ATCAGGGCGC ACCTGGGGGA GGTTCTGGAA 
GCTGTCCGGT ACCTGCACAA CTGCAGGATA GCACACCTGG ACCTAAAGCC TGAGAATATC 
CTGGTGGATG AGAGTTTAGC CAAGCCAACC ATCAAACTGG CTGACTTTGG AGATGCTGTT 
CAGCTCAACA CGACCTACTA CATCCACCAG TTACTGGGGA ACCCTGAATT CGCAGCCCCT 
GAAATCATCC TCGGGAACCC TGTCTCCCTG ACCTCGGATA CGTGGAGTGT TGGAGTGCTC 
ACATACGTAC TTCTTAGTGG CGTGTCCCCC TTCCTGGATG ACAGTGTGGA AGAGACCTGC 
CTGAACATTT GCCGCTTAGA CTTTAGCTTC CCAGATGACT ACTTTAAAGG AGTGAGCCAG 
AAGGCCAAGG AGTTCGTGTG CTTCCTCCTG CAGGAGGACC CCGCCAAGCG TCCCTCGGCT 
GCGCTGGCCC TCCAGGAGCA GTGGCTGCAG GCCGGCAACG GCAGAAGCAC GGGCGTCCTC 
GACACGTCCA GACTGACTTC CTTCATTGAG CGGCGCAAAC ACCAGAATGA TGTTCGACCT 
ATCCGTAGCA TTAAAAACTT TCTGCAGAGC AGGCTTCTGC CTAGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514108.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512410.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

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CloneID	OHu61137
Clone ID Related Accession (Same CDS sequence)	XM_011514108.1
Accession Version	XM_011514108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACTC CGGGTTGGAG AGCGGAGGAG GGCGGGCCAG GGTTTCGAAA AAACGATGAA 
ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512410.1
CDS	16..9192
Translation	MQTPGWRAEEGGPGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514108.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514108.1

ORF Insert Sequence:

ATGCAGACTC CGGGTTGGAG AGCGGAGGAG GGCGGGCCAG GGTTTCGAAA AAACGATGAA 
ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514109.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512411.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

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CloneID	OHu61138
Clone ID Related Accession (Same CDS sequence)	XM_011514109.3 , XM_011514109.2
Accession Version	XM_011514109.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514109.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG GGTTTCGAAA AAACGATGAA ATGAAAGCTA TGGATGTTTT ACCAATTTTG 
AAGGAAAAAG TTGCATACCT TTCAGGTGGG AGAGATAAAC GTGGAGGTCC CATTTTAACG 
TTTCCGGCCC GCAGCAATCA TGACAGAATA CGACAGGAGG ATCTCAGGAG ACTCATTTCC 
TATCTAGCCT GTATTCCCAG CGAGGAGGTC TGCAAGCGTG GCTTCACGGT GATCGTGGAC 
ATGCGTGGGT CCAAGTGGGA CTCCATCAAG CCCCTTCTGA AGATCCTGCA GGAGTCCTTC 
CCCTGCTGCA TCCATGTGGC CCTGATCATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACTAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAATA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTTG ATGGCTGCCT GGAATACAAC 
CACGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCCACCCAC 
ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT 
TTAGAGGGGG CTCGGAATAT GATCGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAG 
GCCCCCATCG AGGACCTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT 
GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA GGCAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTGGA CCGGCTGCAC TCGACACGGC AGCATCTGCA CCAGATGTGG 
CATGTGAGGA AGCTGAAGCT GGACCAGTGC TTCCAGCTGA GGCTGTTTGA ACAGGATGCT 
GAGAAGATGT TTGACTGGAT CACACACAAC AAAGGCCTGT TTCTAAACAG CTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCCATGGAG CTTCAGACGC AGCACAATCA CTTTGCCATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATCATGT CGGTGGCCAA TCGTCTGGTG 
GAGTCTGGCC ACTATGCCTC GCAGCAGATC AGGCAGATCG CGAGTCAGCT GGAGCAGGAG 
TGGAAGGCGT TTGCGGCAGC CCTGGATGAG CGGAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCCGAAAA GTATATGAGC AACGTGGATT CATGGTGTAA AGCTTGCGGT 
GAGGTAGACC TTCCCTCAGA GCTGCAGGAC CTAGAAGATG CCATTCATCA CCACCAGGGA 
ATATATGAAC ATATCACTCT TGCTTATTCT GAGGTCAGCC AAGATGGGAA GTCGCTCCTT 
GACAAGCTCC AGCGGCCCTT GACTCCCGGC AGCTCCGATT CCCTGACAGC CTCTGCCAAC 
TACTCCAAGG CCGTGCACCA TGTCCTGGAT GTCATCCACG AGGTGCTGCA CCACCAGCGG 
CAGCTGGAGA ACATCTGGCA ACACCGCAAG GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA GCAGGTGCTA GACTGGATCG AGAACCACGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCATT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT GGCACAGAAC ACATACACCA ATGCGGATAA ATTACTGGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAA TGTGACCCCG AAGAGATTTA TCAGGCTGCC 
CATCAGCTGG AAGACCGGAT TCAAGATTTC GTTCGGCGTG TTGAGCAGCG AAAGATCCTA 
CTGGACATGT CAGTGTCCTT TCACACCCAT GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
CTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCCGAGTCGG TGGAGGCCGT GCAGGACCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACCCTGCAGG TGACTGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATCCA GCAGCTCAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CACAACAGCT CCATCAACCA CATTGAGACG GTGCTGCAGC AGCTGGACGA GGCGCAGTCG 
CAGATGGAGG AGCTCTTCCA GGAGCGCAAG ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGACATTATC TCAGACCTCG AGTCTTGGAA TGATGAGCTT 
TCTCAGCAAA TGAATGACTT CGACACAGAA GATCTCACGA TTGCAGAGCA GCGCCTCCAG 
CACCATGCAG ACAAAGCCTT GACCATGAAC AACTTGACTT TTGACGTCAT CCACCAAGGG 
CAAGATCTTC TGCAGTATGT CAATGAGGTC CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT 
AGAGATGTAG ACATGGCAAC TCGGGTCCAG GACCTGCTGG AGTTTCTTCA TGAAAAACAG 
CAGGAATTGG ATTTAGCCGC AGAGCAGCAT CGGAAACACC TGGAGCAGTG CGTGCAGCTG 
CGCCACCTGC AGGCAGAAGT GAAACAGGTG CTGGGTTGGA TCCGCAACGG AGAGTCCATG 
TTAAATGCCG GACTTATCAC AGCCAGCTCG TTACAAGAGG CAGAGCAGCT CCAGCGAGAG 
CACGAGCAGT TCCAGCATGC CATTGAGAAA ACACATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCAGAAG CCATGCTACA GGCCAACCAC TACGACATGG ACATGATCCG GGACTGCGCC 
GAGAAGGTGG CGTCTCACTG GCAACAGCTC ATGCTCAAGA TGGAAGATCG CCTCAAGCTC 
GTCAACGCCT CTGTCGCTTT CTACAAAACC TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC 
CTGGAACAGG AGTACAAGAG AGAAGAAGAC TGGTGTGGCG GGGCGGATAA GCTGGGCCCA 
AACTCTGAGA CGGACCACGT GACGCCCATG ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA 
TTCCTGAAGG CTTGCACCCT TGCTCGGAGG AATGCAGACG TCTTCCTGAA ATACCTGCAC 
AGGAACAGCG TGAACATGCC AGGAATGGTG ACGCACATCA AAGCTCCTGA ACAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TATTGCATTA CTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGTCAGCAG TACGTGGTCT TTGAGAGGAG TGCCAAGCAG 
GCTTTGGAAT GGATCCATGA CAATGGCGAG TTCTACCTTT CCACACACAC CTCCACGGGC 
TCCAGTATAC AGCACACCCA GGAGCTCCTG AAAGAGCACG AGGAGTTCCA GATAACTGCA 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA GATAAAAAAA TGTGTTACTG CTGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAGTACAGG ACCTCTTTGG AAAAAGCCCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTC CAGCTAGATA TCATTCCAGC CAGTATCCCT 
GGCTCAGAGG TGAAACTTCG AGATGCTGCT CATGAACTTA ATGAAGAGAA GCGGAAATCT 
GCCCGCAGGA AAGAGTTCAT AATGGCTGAG CTCATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTCCGGG AATGTATGGA TACGTACCTG TGGGAAATGA CCAGTGGCGT GGAAGAGATT 
CCACCTGGCA TTGTAAACAA AGAACTCATC ATCTTCGGAA ACATGCAAGA AATCTACGAA 
TTTCATAATA ACATATTCCT AAAGGAGCTG GAAAAATATG AACAGTTGCC AGAGGATGTT 
GGACATTGTT TTGTTACTTG GGCAGACAAG TTTCAGATGT ATGTCACATA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT GATATTGGAA CATGCAGGGT CCTATTTTGA CGAGATACAG 
CAGCGACATG GATTAGCCAA TTCCATTTCT TCCTACCTTA TTAAACCAGT TCAGCGAATA 
ACGAAGTATC AGCTCCTTTT AAAAGAGCTG CTGACGTGCT GTGAGGAAGG AAAGGGAGAG 
ATTAAAGATG GCCTGGAGGT GATGCTCAGC GTGCCGAAGC GAGCCAATGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAACT CATCCTACAG 
GAATCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AGGGTCGAGA ACGGCATCTC 
TTCCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTGA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ATAAAAGCAA ATTGTTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGAGACCCTT GCAAATTTGC ACTGTGGGTG GGGAGAACAC CAACTTCAGA TAATAAAATT 
GTCCTTAAGG CTTCCAGCAT AGAGAACAAG CAGGACTGGA TAAAGCATAT CCGCGAAGTC 
ATCCAGGAGC GGACGATCCA CCTGAAGGGA GCCCTGAAGG AGCCCATTCA CATCCCTAAG 
ACCGCTCCCG CCACAAGACA GAAGGGAAGG AGGGATGGAG AGGATCTGGA CAGCCAAGGA 
GACGGCAGCA GCCAGCCTGA TACGATTTCC ATCGCCTCAC GGACGTCTCA GAACACGCTG 
GACAGCGATA AGCTCTCTGG TGGCTGTGAG CTGACAGTGG TGATCCATGA CTTCACCGCT 
TGCAACAGCA ACGAGCTGAC CATCCGACGG GGCCAGACCG TGGAAGTTCT GGAGCGGCCG 
CATGACAAGC CTGACTGGTG TCTGGTGCGG ACAACTGACC GCTCCCCAGC GGCAGAAGGC 
CTGGTCCCCT GTGGTTCACT GTGCATCGCC CACTCCAGAA GTAGCATGGA AATGGAGGGC 
ATCTTCAACC ACAAAGACTC GCTCTCCGTC TCCAGCAATG ACGCCAGTCC ACCCGCATCC 
GTGGCTTCCC TCCAGCCCCA CATGATCGGG GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG 
GGCAACACCC TGCGCAAGTG GCTCACCAGC CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC 
GACGGGCACG TGAAGAAGCT GGCGCACAAG CACAAGAAGA GCCGCGAGGT CCGCAAGAGC 
GCCGACGCCG GCTCGCAGAA GGACTCCGAC GACAGTGCGG CCACCCCGCA GGACGAGACG 
GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC 
TCGGGGATGC AGAGCTGTGG AGAAGAGGAA GGCGAGGAGG GGGCCGACGC CGTGCCCCTG 
CCGCCACCCA TGGCCATCCA GCAGCACAGC CTCCTCCAGC CAGACTCACA GGATGACAAG 
GCCTCTTCTC GGTTATTAGT CCGCCCCACC AGCTCCGAAA CACCGAGTGC AGCCGAGCTC 
GTCAGTGCAA TTGAGGAACT CGTGAAAAGC AAGATGGCAC TGGAGGATCG CCCCAGCTCA 
CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC CCTTCCTTCA ACCCTTCGGA TAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGACACTACG TTTTGCAAGA ACTAGTGGAG ACAGAGCGTG ACTATGTGCG GGACCTTGGC 
TATGTGGTTG AGGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAA 
GGAAAAGACA AAATTGTGTT CGGCAACATC CATCAGATTT ACGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA GATCCAGAAA AACTAGGATC CCTTTTTGTT 
AAACACGAGA GAAGGTTGCA CATGTACATA GCTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAGCG TCTTGGCCAC 
AGGTTACAGC TCACAGATCT GTTGATCAAA CCAGTGCAGA GAATCATGAA GTATCAGCTG 
TTACTGAAGG ACTTCCTCAA GTATTCCAAA AAGGCCAGCC TGGATACATC AGAATTAGAG 
AGAGCTGTGG AAGTCATGTG CATAGTACCC AGGCGGTGCA ACGACATGAT GAACGTGGGG 
CGGCTGCAAG GATTCGACGG GAAAATCGTT GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTCTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAGAGAGAG GCGCATCTTC 
CTCTTTGAGC AGATCGTCAT ATTCAGCGAA CCACTTGATA AAAAGAAGGG CTTCTCCATG 
CCGGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CCTGTAAATT TGCTCTGACA TCGAGGACGG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCTAGTCC AAGTGTCCGG CAAACTTGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCGC CAATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCGG CGGCGGCAGC GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC 
GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC CACAGTGGCG GCCCCAGCAG CTGCGGCGGC 
GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC CGGATCCCCC AGCCTGTCCG ACACCACCCC 
CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG 
TCCACCCCCG GGCCCTCCCT GCCTCCCCCT GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG 
CCCAGCAGGC GGCCCCCCGG CGCGGACGCC GAGGGGTCCG AGCGAGAAGC GGAGCCGATC 
CCCAAGATGA AGGTGCTGGA GAGCCCCAGG AAAGGCGCCG CGAACGCCTC GGGGTCGAGC 
CCAGACGCCC CCGCCAAGGA CGCGCGCGCT AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC 
CGGGCCGGGG CCGCTTCGCC GCTGAACTCG CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC 
AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG CAGAAGGGGG GCTCCTTCTG GAGCTCCATC 
CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC TTCACCTTCC CGGGGGACAG CGACTCCCTC 
CAGCGGCAGA CACCCCGCCA CGCGGCCCCT GGCAAGGATA CTGACCGCAT GAGCACGTGC 
TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC 
AGTAGCAACA TCTCCACCAT GTTGGTGACA CACGATTACA CGGCAGTGAA GGAGGATGAG 
ATCAACGTCT ACCAAGGAGA GGTCGTTCAA ATTCTGGCCA GCAACCAGCA GAACATGTTT 
CTGGTGTTCC GAGCCGCCAC TGACCAGTGC CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT 
GTCCTGGGCC ACACCAGTGC AGTCATCGTG GAGAACCCGG ACGGGACTCT CAAGAAGTCA 
ACATCTTGGC ACACAGCACT CCGTTTAAGG AAAAAATCTG AGAAAAAAGA TAAAGACGGC 
AAAAGGGAAG GCAAGTTAGA GAACGGTTAT CGGAAGTCAC GGGAAGGACT CAGCAACAAG 
GTATCTGTGA AGCTTCTCAA TCCCAACTAC ATTTATGACG TTCCCCCAGA ATTCGTCATT 
CCATTGAGTG AGGTCACGTG TGAGACAGGG GAGACCGTTG TTCTTAGATG TCGAGTCTGT 
GGCCGCCCCA AAGCCTCAAT TACCTGGAAG GGCCCTGAAC ACAACACCTT GAACAACGAT 
GGTCACTACA GCATCTCCTA CAGTGACCTG GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG 
ACCACGGAAG ATGACGGCAT CTACACGTGC ATCGCTGTCA ATGACATGGG TTCAGCCTCA 
TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA GGGATGGATG GGATCATGGT GACCTGGAAA 
GACAACTTTG ACTCCTTCTA CAGTGAAGTG GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC 
GTTAAGAAAT GTGATCAGAA AGGAACCAAG CGAGCAGTGG CCACTAAGTT TGTGAACAAG 
AAGTTGATGA AGCGCGACCA GGTCACCCAT GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC 
CCCCTGCTTG TCGGCCTCCT CGACACCTTT GAGACCCCCA CCAGCTACAT CCTGGTCTTA 
GAAATGGCTG ACCAGGGTCG CCTCCTGGAC TGCGTGGTGC GATGGGGAAG CCTCACTGAA 
GGGAAGATCA GGGCGCACCT GGGGGAGGTT CTGGAAGCTG TCCGGTACCT GCACAACTGC 
AGGATAGCAC ACCTGGACCT AAAGCCTGAG AATATCCTGG TGGATGAGAG TTTAGCCAAG 
CCAACCATCA AACTGGCTGA CTTTGGAGAT GCTGTTCAGC TCAACACGAC CTACTACATC 
CACCAGTTAC TGGGGAACCC TGAATTCGCA GCCCCTGAAA TCATCCTCGG GAACCCTGTC 
TCCCTGACCT CGGATACGTG GAGTGTTGGA GTGCTCACAT ACGTACTTCT TAGTGGCGTG 
TCCCCCTTCC TGGATGACAG TGTGGAAGAG ACCTGCCTGA ACATTTGCCG CTTAGACTTT 
AGCTTCCCAG ATGACTACTT TAAAGGAGTG AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC 
CTCCTGCAGG AGGACCCCGC CAAGCGTCCC TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG 
CTGCAGGCCG GCAACGGCAG AAGCACGGGC GTCCTCGACA CGTCCAGACT GACTTCCTTC 
ATTGAGCGGC GCAAACACCA GAATGATGTT CGACCTATCC GTAGCATTAA AAACTTTCTG 
CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512411.1
CDS	7372..16518
Translation	MAEGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514109.3
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514109.3

ORF Insert Sequence:

ATGGCAGAAG GGTTTCGAAA AAACGATGAA ATGAAAGCTA TGGATGTTTT ACCAATTTTG 
AAGGAAAAAG TTGCATACCT TTCAGGTGGG AGAGATAAAC GTGGAGGTCC CATTTTAACG 
TTTCCGGCCC GCAGCAATCA TGACAGAATA CGACAGGAGG ATCTCAGGAG ACTCATTTCC 
TATCTAGCCT GTATTCCCAG CGAGGAGGTC TGCAAGCGTG GCTTCACGGT GATCGTGGAC 
ATGCGTGGGT CCAAGTGGGA CTCCATCAAG CCCCTTCTGA AGATCCTGCA GGAGTCCTTC 
CCCTGCTGCA TCCATGTGGC CCTGATCATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACTAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAATA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTTG ATGGCTGCCT GGAATACAAC 
CACGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCCACCCAC 
ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT 
TTAGAGGGGG CTCGGAATAT GATCGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAG 
GCCCCCATCG AGGACCTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT 
GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA GGCAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTGGA CCGGCTGCAC TCGACACGGC AGCATCTGCA CCAGATGTGG 
CATGTGAGGA AGCTGAAGCT GGACCAGTGC TTCCAGCTGA GGCTGTTTGA ACAGGATGCT 
GAGAAGATGT TTGACTGGAT CACACACAAC AAAGGCCTGT TTCTAAACAG CTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCCATGGAG CTTCAGACGC AGCACAATCA CTTTGCCATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATCATGT CGGTGGCCAA TCGTCTGGTG 
GAGTCTGGCC ACTATGCCTC GCAGCAGATC AGGCAGATCG CGAGTCAGCT GGAGCAGGAG 
TGGAAGGCGT TTGCGGCAGC CCTGGATGAG CGGAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCCGAAAA GTATATGAGC AACGTGGATT CATGGTGTAA AGCTTGCGGT 
GAGGTAGACC TTCCCTCAGA GCTGCAGGAC CTAGAAGATG CCATTCATCA CCACCAGGGA 
ATATATGAAC ATATCACTCT TGCTTATTCT GAGGTCAGCC AAGATGGGAA GTCGCTCCTT 
GACAAGCTCC AGCGGCCCTT GACTCCCGGC AGCTCCGATT CCCTGACAGC CTCTGCCAAC 
TACTCCAAGG CCGTGCACCA TGTCCTGGAT GTCATCCACG AGGTGCTGCA CCACCAGCGG 
CAGCTGGAGA ACATCTGGCA ACACCGCAAG GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA GCAGGTGCTA GACTGGATCG AGAACCACGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCATT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT GGCACAGAAC ACATACACCA ATGCGGATAA ATTACTGGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAA TGTGACCCCG AAGAGATTTA TCAGGCTGCC 
CATCAGCTGG AAGACCGGAT TCAAGATTTC GTTCGGCGTG TTGAGCAGCG AAAGATCCTA 
CTGGACATGT CAGTGTCCTT TCACACCCAT GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
CTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCCGAGTCGG TGGAGGCCGT GCAGGACCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACCCTGCAGG TGACTGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATCCA GCAGCTCAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CACAACAGCT CCATCAACCA CATTGAGACG GTGCTGCAGC AGCTGGACGA GGCGCAGTCG 
CAGATGGAGG AGCTCTTCCA GGAGCGCAAG ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGACATTATC TCAGACCTCG AGTCTTGGAA TGATGAGCTT 
TCTCAGCAAA TGAATGACTT CGACACAGAA GATCTCACGA TTGCAGAGCA GCGCCTCCAG 
CACCATGCAG ACAAAGCCTT GACCATGAAC AACTTGACTT TTGACGTCAT CCACCAAGGG 
CAAGATCTTC TGCAGTATGT CAATGAGGTC CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT 
AGAGATGTAG ACATGGCAAC TCGGGTCCAG GACCTGCTGG AGTTTCTTCA TGAAAAACAG 
CAGGAATTGG ATTTAGCCGC AGAGCAGCAT CGGAAACACC TGGAGCAGTG CGTGCAGCTG 
CGCCACCTGC AGGCAGAAGT GAAACAGGTG CTGGGTTGGA TCCGCAACGG AGAGTCCATG 
TTAAATGCCG GACTTATCAC AGCCAGCTCG TTACAAGAGG CAGAGCAGCT CCAGCGAGAG 
CACGAGCAGT TCCAGCATGC CATTGAGAAA ACACATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCAGAAG CCATGCTACA GGCCAACCAC TACGACATGG ACATGATCCG GGACTGCGCC 
GAGAAGGTGG CGTCTCACTG GCAACAGCTC ATGCTCAAGA TGGAAGATCG CCTCAAGCTC 
GTCAACGCCT CTGTCGCTTT CTACAAAACC TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC 
CTGGAACAGG AGTACAAGAG AGAAGAAGAC TGGTGTGGCG GGGCGGATAA GCTGGGCCCA 
AACTCTGAGA CGGACCACGT GACGCCCATG ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA 
TTCCTGAAGG CTTGCACCCT TGCTCGGAGG AATGCAGACG TCTTCCTGAA ATACCTGCAC 
AGGAACAGCG TGAACATGCC AGGAATGGTG ACGCACATCA AAGCTCCTGA ACAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TATTGCATTA CTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGTCAGCAG TACGTGGTCT TTGAGAGGAG TGCCAAGCAG 
GCTTTGGAAT GGATCCATGA CAATGGCGAG TTCTACCTTT CCACACACAC CTCCACGGGC 
TCCAGTATAC AGCACACCCA GGAGCTCCTG AAAGAGCACG AGGAGTTCCA GATAACTGCA 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA GATAAAAAAA TGTGTTACTG CTGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAGTACAGG ACCTCTTTGG AAAAAGCCCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTC CAGCTAGATA TCATTCCAGC CAGTATCCCT 
GGCTCAGAGG TGAAACTTCG AGATGCTGCT CATGAACTTA ATGAAGAGAA GCGGAAATCT 
GCCCGCAGGA AAGAGTTCAT AATGGCTGAG CTCATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTCCGGG AATGTATGGA TACGTACCTG TGGGAAATGA CCAGTGGCGT GGAAGAGATT 
CCACCTGGCA TTGTAAACAA AGAACTCATC ATCTTCGGAA ACATGCAAGA AATCTACGAA 
TTTCATAATA ACATATTCCT AAAGGAGCTG GAAAAATATG AACAGTTGCC AGAGGATGTT 
GGACATTGTT TTGTTACTTG GGCAGACAAG TTTCAGATGT ATGTCACATA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT GATATTGGAA CATGCAGGGT CCTATTTTGA CGAGATACAG 
CAGCGACATG GATTAGCCAA TTCCATTTCT TCCTACCTTA TTAAACCAGT TCAGCGAATA 
ACGAAGTATC AGCTCCTTTT AAAAGAGCTG CTGACGTGCT GTGAGGAAGG AAAGGGAGAG 
ATTAAAGATG GCCTGGAGGT GATGCTCAGC GTGCCGAAGC GAGCCAATGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAACT CATCCTACAG 
GAATCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AGGGTCGAGA ACGGCATCTC 
TTCCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTGA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ATAAAAGCAA ATTGTTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGAGACCCTT GCAAATTTGC ACTGTGGGTG GGGAGAACAC CAACTTCAGA TAATAAAATT 
GTCCTTAAGG CTTCCAGCAT AGAGAACAAG CAGGACTGGA TAAAGCATAT CCGCGAAGTC 
ATCCAGGAGC GGACGATCCA CCTGAAGGGA GCCCTGAAGG AGCCCATTCA CATCCCTAAG 
ACCGCTCCCG CCACAAGACA GAAGGGAAGG AGGGATGGAG AGGATCTGGA CAGCCAAGGA 
GACGGCAGCA GCCAGCCTGA TACGATTTCC ATCGCCTCAC GGACGTCTCA GAACACGCTG 
GACAGCGATA AGCTCTCTGG TGGCTGTGAG CTGACAGTGG TGATCCATGA CTTCACCGCT 
TGCAACAGCA ACGAGCTGAC CATCCGACGG GGCCAGACCG TGGAAGTTCT GGAGCGGCCG 
CATGACAAGC CTGACTGGTG TCTGGTGCGG ACAACTGACC GCTCCCCAGC GGCAGAAGGC 
CTGGTCCCCT GTGGTTCACT GTGCATCGCC CACTCCAGAA GTAGCATGGA AATGGAGGGC 
ATCTTCAACC ACAAAGACTC GCTCTCCGTC TCCAGCAATG ACGCCAGTCC ACCCGCATCC 
GTGGCTTCCC TCCAGCCCCA CATGATCGGG GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG 
GGCAACACCC TGCGCAAGTG GCTCACCAGC CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC 
GACGGGCACG TGAAGAAGCT GGCGCACAAG CACAAGAAGA GCCGCGAGGT CCGCAAGAGC 
GCCGACGCCG GCTCGCAGAA GGACTCCGAC GACAGTGCGG CCACCCCGCA GGACGAGACG 
GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC 
TCGGGGATGC AGAGCTGTGG AGAAGAGGAA GGCGAGGAGG GGGCCGACGC CGTGCCCCTG 
CCGCCACCCA TGGCCATCCA GCAGCACAGC CTCCTCCAGC CAGACTCACA GGATGACAAG 
GCCTCTTCTC GGTTATTAGT CCGCCCCACC AGCTCCGAAA CACCGAGTGC AGCCGAGCTC 
GTCAGTGCAA TTGAGGAACT CGTGAAAAGC AAGATGGCAC TGGAGGATCG CCCCAGCTCA 
CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC CCTTCCTTCA ACCCTTCGGA TAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGACACTACG TTTTGCAAGA ACTAGTGGAG ACAGAGCGTG ACTATGTGCG GGACCTTGGC 
TATGTGGTTG AGGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAA 
GGAAAAGACA AAATTGTGTT CGGCAACATC CATCAGATTT ACGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA GATCCAGAAA AACTAGGATC CCTTTTTGTT 
AAACACGAGA GAAGGTTGCA CATGTACATA GCTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAGCG TCTTGGCCAC 
AGGTTACAGC TCACAGATCT GTTGATCAAA CCAGTGCAGA GAATCATGAA GTATCAGCTG 
TTACTGAAGG ACTTCCTCAA GTATTCCAAA AAGGCCAGCC TGGATACATC AGAATTAGAG 
AGAGCTGTGG AAGTCATGTG CATAGTACCC AGGCGGTGCA ACGACATGAT GAACGTGGGG 
CGGCTGCAAG GATTCGACGG GAAAATCGTT GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTCTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAGAGAGAG GCGCATCTTC 
CTCTTTGAGC AGATCGTCAT ATTCAGCGAA CCACTTGATA AAAAGAAGGG CTTCTCCATG 
CCGGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CCTGTAAATT TGCTCTGACA TCGAGGACGG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCTAGTCC AAGTGTCCGG CAAACTTGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCGC CAATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCGG CGGCGGCAGC GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC 
GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC CACAGTGGCG GCCCCAGCAG CTGCGGCGGC 
GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC CGGATCCCCC AGCCTGTCCG ACACCACCCC 
CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG 
TCCACCCCCG GGCCCTCCCT GCCTCCCCCT GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG 
CCCAGCAGGC GGCCCCCCGG CGCGGACGCC GAGGGGTCCG AGCGAGAAGC GGAGCCGATC 
CCCAAGATGA AGGTGCTGGA GAGCCCCAGG AAAGGCGCCG CGAACGCCTC GGGGTCGAGC 
CCAGACGCCC CCGCCAAGGA CGCGCGCGCT AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC 
CGGGCCGGGG CCGCTTCGCC GCTGAACTCG CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC 
AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG CAGAAGGGGG GCTCCTTCTG GAGCTCCATC 
CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC TTCACCTTCC CGGGGGACAG CGACTCCCTC 
CAGCGGCAGA CACCCCGCCA CGCGGCCCCT GGCAAGGATA CTGACCGCAT GAGCACGTGC 
TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC 
AGTAGCAACA TCTCCACCAT GTTGGTGACA CACGATTACA CGGCAGTGAA GGAGGATGAG 
ATCAACGTCT ACCAAGGAGA GGTCGTTCAA ATTCTGGCCA GCAACCAGCA GAACATGTTT 
CTGGTGTTCC GAGCCGCCAC TGACCAGTGC CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT 
GTCCTGGGCC ACACCAGTGC AGTCATCGTG GAGAACCCGG ACGGGACTCT CAAGAAGTCA 
ACATCTTGGC ACACAGCACT CCGTTTAAGG AAAAAATCTG AGAAAAAAGA TAAAGACGGC 
AAAAGGGAAG GCAAGTTAGA GAACGGTTAT CGGAAGTCAC GGGAAGGACT CAGCAACAAG 
GTATCTGTGA AGCTTCTCAA TCCCAACTAC ATTTATGACG TTCCCCCAGA ATTCGTCATT 
CCATTGAGTG AGGTCACGTG TGAGACAGGG GAGACCGTTG TTCTTAGATG TCGAGTCTGT 
GGCCGCCCCA AAGCCTCAAT TACCTGGAAG GGCCCTGAAC ACAACACCTT GAACAACGAT 
GGTCACTACA GCATCTCCTA CAGTGACCTG GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG 
ACCACGGAAG ATGACGGCAT CTACACGTGC ATCGCTGTCA ATGACATGGG TTCAGCCTCA 
TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA GGGATGGATG GGATCATGGT GACCTGGAAA 
GACAACTTTG ACTCCTTCTA CAGTGAAGTG GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC 
GTTAAGAAAT GTGATCAGAA AGGAACCAAG CGAGCAGTGG CCACTAAGTT TGTGAACAAG 
AAGTTGATGA AGCGCGACCA GGTCACCCAT GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC 
CCCCTGCTTG TCGGCCTCCT CGACACCTTT GAGACCCCCA CCAGCTACAT CCTGGTCTTA 
GAAATGGCTG ACCAGGGTCG CCTCCTGGAC TGCGTGGTGC GATGGGGAAG CCTCACTGAA 
GGGAAGATCA GGGCGCACCT GGGGGAGGTT CTGGAAGCTG TCCGGTACCT GCACAACTGC 
AGGATAGCAC ACCTGGACCT AAAGCCTGAG AATATCCTGG TGGATGAGAG TTTAGCCAAG 
CCAACCATCA AACTGGCTGA CTTTGGAGAT GCTGTTCAGC TCAACACGAC CTACTACATC 
CACCAGTTAC TGGGGAACCC TGAATTCGCA GCCCCTGAAA TCATCCTCGG GAACCCTGTC 
TCCCTGACCT CGGATACGTG GAGTGTTGGA GTGCTCACAT ACGTACTTCT TAGTGGCGTG 
TCCCCCTTCC TGGATGACAG TGTGGAAGAG ACCTGCCTGA ACATTTGCCG CTTAGACTTT 
AGCTTCCCAG ATGACTACTT TAAAGGAGTG AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC 
CTCCTGCAGG AGGACCCCGC CAAGCGTCCC TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG 
CTGCAGGCCG GCAACGGCAG AAGCACGGGC GTCCTCGACA CGTCCAGACT GACTTCCTTC 
ATTGAGCGGC GCAAACACCA GAATGATGTT CGACCTATCC GTAGCATTAA AAACTTTCTG 
CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514109.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512411.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

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CloneID	OHu61138
Clone ID Related Accession (Same CDS sequence)	XM_011514109.3 , XM_011514109.2
Accession Version	XM_011514109.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG GGTTTCGAAA AAACGATGAA ATGAAAGCTA TGGATGTTTT ACCAATTTTG 
AAGGAAAAAG TTGCATACCT TTCAGGTGGG AGAGATAAAC GTGGAGGTCC CATTTTAACG 
TTTCCGGCCC GCAGCAATCA TGACAGAATA CGACAGGAGG ATCTCAGGAG ACTCATTTCC 
TATCTAGCCT GTATTCCCAG CGAGGAGGTC TGCAAGCGTG GCTTCACGGT GATCGTGGAC 
ATGCGTGGGT CCAAGTGGGA CTCCATCAAG CCCCTTCTGA AGATCCTGCA GGAGTCCTTC 
CCCTGCTGCA TCCATGTGGC CCTGATCATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACTAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAATA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTTG ATGGCTGCCT GGAATACAAC 
CACGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCCACCCAC 
ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT 
TTAGAGGGGG CTCGGAATAT GATCGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAG 
GCCCCCATCG AGGACCTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT 
GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA GGCAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTGGA CCGGCTGCAC TCGACACGGC AGCATCTGCA CCAGATGTGG 
CATGTGAGGA AGCTGAAGCT GGACCAGTGC TTCCAGCTGA GGCTGTTTGA ACAGGATGCT 
GAGAAGATGT TTGACTGGAT CACACACAAC AAAGGCCTGT TTCTAAACAG CTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCCATGGAG CTTCAGACGC AGCACAATCA CTTTGCCATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATCATGT CGGTGGCCAA TCGTCTGGTG 
GAGTCTGGCC ACTATGCCTC GCAGCAGATC AGGCAGATCG CGAGTCAGCT GGAGCAGGAG 
TGGAAGGCGT TTGCGGCAGC CCTGGATGAG CGGAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCCGAAAA GTATATGAGC AACGTGGATT CATGGTGTAA AGCTTGCGGT 
GAGGTAGACC TTCCCTCAGA GCTGCAGGAC CTAGAAGATG CCATTCATCA CCACCAGGGA 
ATATATGAAC ATATCACTCT TGCTTATTCT GAGGTCAGCC AAGATGGGAA GTCGCTCCTT 
GACAAGCTCC AGCGGCCCTT GACTCCCGGC AGCTCCGATT CCCTGACAGC CTCTGCCAAC 
TACTCCAAGG CCGTGCACCA TGTCCTGGAT GTCATCCACG AGGTGCTGCA CCACCAGCGG 
CAGCTGGAGA ACATCTGGCA ACACCGCAAG GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA GCAGGTGCTA GACTGGATCG AGAACCACGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCATT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT GGCACAGAAC ACATACACCA ATGCGGATAA ATTACTGGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAA TGTGACCCCG AAGAGATTTA TCAGGCTGCC 
CATCAGCTGG AAGACCGGAT TCAAGATTTC GTTCGGCGTG TTGAGCAGCG AAAGATCCTA 
CTGGACATGT CAGTGTCCTT TCACACCCAT GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
CTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCCGAGTCGG TGGAGGCCGT GCAGGACCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACCCTGCAGG TGACTGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATCCA GCAGCTCAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CACAACAGCT CCATCAACCA CATTGAGACG GTGCTGCAGC AGCTGGACGA GGCGCAGTCG 
CAGATGGAGG AGCTCTTCCA GGAGCGCAAG ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGACATTATC TCAGACCTCG AGTCTTGGAA TGATGAGCTT 
TCTCAGCAAA TGAATGACTT CGACACAGAA GATCTCACGA TTGCAGAGCA GCGCCTCCAG 
CACCATGCAG ACAAAGCCTT GACCATGAAC AACTTGACTT TTGACGTCAT CCACCAAGGG 
CAAGATCTTC TGCAGTATGT CAATGAGGTC CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT 
AGAGATGTAG ACATGGCAAC TCGGGTCCAG GACCTGCTGG AGTTTCTTCA TGAAAAACAG 
CAGGAATTGG ATTTAGCCGC AGAGCAGCAT CGGAAACACC TGGAGCAGTG CGTGCAGCTG 
CGCCACCTGC AGGCAGAAGT GAAACAGGTG CTGGGTTGGA TCCGCAACGG AGAGTCCATG 
TTAAATGCCG GACTTATCAC AGCCAGCTCG TTACAAGAGG CAGAGCAGCT CCAGCGAGAG 
CACGAGCAGT TCCAGCATGC CATTGAGAAA ACACATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCAGAAG CCATGCTACA GGCCAACCAC TACGACATGG ACATGATCCG GGACTGCGCC 
GAGAAGGTGG CGTCTCACTG GCAACAGCTC ATGCTCAAGA TGGAAGATCG CCTCAAGCTC 
GTCAACGCCT CTGTCGCTTT CTACAAAACC TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC 
CTGGAACAGG AGTACAAGAG AGAAGAAGAC TGGTGTGGCG GGGCGGATAA GCTGGGCCCA 
AACTCTGAGA CGGACCACGT GACGCCCATG ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA 
TTCCTGAAGG CTTGCACCCT TGCTCGGAGG AATGCAGACG TCTTCCTGAA ATACCTGCAC 
AGGAACAGCG TGAACATGCC AGGAATGGTG ACGCACATCA AAGCTCCTGA ACAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TATTGCATTA CTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGTCAGCAG TACGTGGTCT TTGAGAGGAG TGCCAAGCAG 
GCTTTGGAAT GGATCCATGA CAATGGCGAG TTCTACCTTT CCACACACAC CTCCACGGGC 
TCCAGTATAC AGCACACCCA GGAGCTCCTG AAAGAGCACG AGGAGTTCCA GATAACTGCA 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA GATAAAAAAA TGTGTTACTG CTGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAGTACAGG ACCTCTTTGG AAAAAGCCCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTC CAGCTAGATA TCATTCCAGC CAGTATCCCT 
GGCTCAGAGG TGAAACTTCG AGATGCTGCT CATGAACTTA ATGAAGAGAA GCGGAAATCT 
GCCCGCAGGA AAGAGTTCAT AATGGCTGAG CTCATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTCCGGG AATGTATGGA TACGTACCTG TGGGAAATGA CCAGTGGCGT GGAAGAGATT 
CCACCTGGCA TTGTAAACAA AGAACTCATC ATCTTCGGAA ACATGCAAGA AATCTACGAA 
TTTCATAATA ACATATTCCT AAAGGAGCTG GAAAAATATG AACAGTTGCC AGAGGATGTT 
GGACATTGTT TTGTTACTTG GGCAGACAAG TTTCAGATGT ATGTCACATA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT GATATTGGAA CATGCAGGGT CCTATTTTGA CGAGATACAG 
CAGCGACATG GATTAGCCAA TTCCATTTCT TCCTACCTTA TTAAACCAGT TCAGCGAATA 
ACGAAGTATC AGCTCCTTTT AAAAGAGCTG CTGACGTGCT GTGAGGAAGG AAAGGGAGAG 
ATTAAAGATG GCCTGGAGGT GATGCTCAGC GTGCCGAAGC GAGCCAATGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAACT CATCCTACAG 
GAATCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AGGGTCGAGA ACGGCATCTC 
TTCCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTGA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ATAAAAGCAA ATTGTTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGAGACCCTT GCAAATTTGC ACTGTGGGTG GGGAGAACAC CAACTTCAGA TAATAAAATT 
GTCCTTAAGG CTTCCAGCAT AGAGAACAAG CAGGACTGGA TAAAGCATAT CCGCGAAGTC 
ATCCAGGAGC GGACGATCCA CCTGAAGGGA GCCCTGAAGG AGCCCATTCA CATCCCTAAG 
ACCGCTCCCG CCACAAGACA GAAGGGAAGG AGGGATGGAG AGGATCTGGA CAGCCAAGGA 
GACGGCAGCA GCCAGCCTGA TACGATTTCC ATCGCCTCAC GGACGTCTCA GAACACGCTG 
GACAGCGATA AGCTCTCTGG TGGCTGTGAG CTGACAGTGG TGATCCATGA CTTCACCGCT 
TGCAACAGCA ACGAGCTGAC CATCCGACGG GGCCAGACCG TGGAAGTTCT GGAGCGGCCG 
CATGACAAGC CTGACTGGTG TCTGGTGCGG ACAACTGACC GCTCCCCAGC GGCAGAAGGC 
CTGGTCCCCT GTGGTTCACT GTGCATCGCC CACTCCAGAA GTAGCATGGA AATGGAGGGC 
ATCTTCAACC ACAAAGACTC GCTCTCCGTC TCCAGCAATG ACGCCAGTCC ACCCGCATCC 
GTGGCTTCCC TCCAGCCCCA CATGATCGGG GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG 
GGCAACACCC TGCGCAAGTG GCTCACCAGC CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC 
GACGGGCACG TGAAGAAGCT GGCGCACAAG CACAAGAAGA GCCGCGAGGT CCGCAAGAGC 
GCCGACGCCG GCTCGCAGAA GGACTCCGAC GACAGTGCGG CCACCCCGCA GGACGAGACG 
GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC 
TCGGGGATGC AGAGCTGTGG AGAAGAGGAA GGCGAGGAGG GGGCCGACGC CGTGCCCCTG 
CCGCCACCCA TGGCCATCCA GCAGCACAGC CTCCTCCAGC CAGACTCACA GGATGACAAG 
GCCTCTTCTC GGTTATTAGT CCGCCCCACC AGCTCCGAAA CACCGAGTGC AGCCGAGCTC 
GTCAGTGCAA TTGAGGAACT CGTGAAAAGC AAGATGGCAC TGGAGGATCG CCCCAGCTCA 
CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC CCTTCCTTCA ACCCTTCGGA TAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGACACTACG TTTTGCAAGA ACTAGTGGAG ACAGAGCGTG ACTATGTGCG GGACCTTGGC 
TATGTGGTTG AGGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAA 
GGAAAAGACA AAATTGTGTT CGGCAACATC CATCAGATTT ACGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA GATCCAGAAA AACTAGGATC CCTTTTTGTT 
AAACACGAGA GAAGGTTGCA CATGTACATA GCTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAGCG TCTTGGCCAC 
AGGTTACAGC TCACAGATCT GTTGATCAAA CCAGTGCAGA GAATCATGAA GTATCAGCTG 
TTACTGAAGG ACTTCCTCAA GTATTCCAAA AAGGCCAGCC TGGATACATC AGAATTAGAG 
AGAGCTGTGG AAGTCATGTG CATAGTACCC AGGCGGTGCA ACGACATGAT GAACGTGGGG 
CGGCTGCAAG GATTCGACGG GAAAATCGTT GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTCTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAGAGAGAG GCGCATCTTC 
CTCTTTGAGC AGATCGTCAT ATTCAGCGAA CCACTTGATA AAAAGAAGGG CTTCTCCATG 
CCGGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CCTGTAAATT TGCTCTGACA TCGAGGACGG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCTAGTCC AAGTGTCCGG CAAACTTGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCGC CAATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCGG CGGCGGCAGC GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC 
GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC CACAGTGGCG GCCCCAGCAG CTGCGGCGGC 
GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC CGGATCCCCC AGCCTGTCCG ACACCACCCC 
CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG 
TCCACCCCCG GGCCCTCCCT GCCTCCCCCT GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG 
CCCAGCAGGC GGCCCCCCGG CGCGGACGCC GAGGGGTCCG AGCGAGAAGC GGAGCCGATC 
CCCAAGATGA AGGTGCTGGA GAGCCCCAGG AAAGGCGCCG CGAACGCCTC GGGGTCGAGC 
CCAGACGCCC CCGCCAAGGA CGCGCGCGCT AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC 
CGGGCCGGGG CCGCTTCGCC GCTGAACTCG CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC 
AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG CAGAAGGGGG GCTCCTTCTG GAGCTCCATC 
CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC TTCACCTTCC CGGGGGACAG CGACTCCCTC 
CAGCGGCAGA CACCCCGCCA CGCGGCCCCT GGCAAGGATA CTGACCGCAT GAGCACGTGC 
TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC 
AGTAGCAACA TCTCCACCAT GTTGGTGACA CACGATTACA CGGCAGTGAA GGAGGATGAG 
ATCAACGTCT ACCAAGGAGA GGTCGTTCAA ATTCTGGCCA GCAACCAGCA GAACATGTTT 
CTGGTGTTCC GAGCCGCCAC TGACCAGTGC CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT 
GTCCTGGGCC ACACCAGTGC AGTCATCGTG GAGAACCCGG ACGGGACTCT CAAGAAGTCA 
ACATCTTGGC ACACAGCACT CCGTTTAAGG AAAAAATCTG AGAAAAAAGA TAAAGACGGC 
AAAAGGGAAG GCAAGTTAGA GAACGGTTAT CGGAAGTCAC GGGAAGGACT CAGCAACAAG 
GTATCTGTGA AGCTTCTCAA TCCCAACTAC ATTTATGACG TTCCCCCAGA ATTCGTCATT 
CCATTGAGTG AGGTCACGTG TGAGACAGGG GAGACCGTTG TTCTTAGATG TCGAGTCTGT 
GGCCGCCCCA AAGCCTCAAT TACCTGGAAG GGCCCTGAAC ACAACACCTT GAACAACGAT 
GGTCACTACA GCATCTCCTA CAGTGACCTG GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG 
ACCACGGAAG ATGACGGCAT CTACACGTGC ATCGCTGTCA ATGACATGGG TTCAGCCTCA 
TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA GGGATGGATG GGATCATGGT GACCTGGAAA 
GACAACTTTG ACTCCTTCTA CAGTGAAGTG GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC 
GTTAAGAAAT GTGATCAGAA AGGAACCAAG CGAGCAGTGG CCACTAAGTT TGTGAACAAG 
AAGTTGATGA AGCGCGACCA GGTCACCCAT GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC 
CCCCTGCTTG TCGGCCTCCT CGACACCTTT GAGACCCCCA CCAGCTACAT CCTGGTCTTA 
GAAATGGCTG ACCAGGGTCG CCTCCTGGAC TGCGTGGTGC GATGGGGAAG CCTCACTGAA 
GGGAAGATCA GGGCGCACCT GGGGGAGGTT CTGGAAGCTG TCCGGTACCT GCACAACTGC 
AGGATAGCAC ACCTGGACCT AAAGCCTGAG AATATCCTGG TGGATGAGAG TTTAGCCAAG 
CCAACCATCA AACTGGCTGA CTTTGGAGAT GCTGTTCAGC TCAACACGAC CTACTACATC 
CACCAGTTAC TGGGGAACCC TGAATTCGCA GCCCCTGAAA TCATCCTCGG GAACCCTGTC 
TCCCTGACCT CGGATACGTG GAGTGTTGGA GTGCTCACAT ACGTACTTCT TAGTGGCGTG 
TCCCCCTTCC TGGATGACAG TGTGGAAGAG ACCTGCCTGA ACATTTGCCG CTTAGACTTT 
AGCTTCCCAG ATGACTACTT TAAAGGAGTG AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC 
CTCCTGCAGG AGGACCCCGC CAAGCGTCCC TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG 
CTGCAGGCCG GCAACGGCAG AAGCACGGGC GTCCTCGACA CGTCCAGACT GACTTCCTTC 
ATTGAGCGGC GCAAACACCA GAATGATGTT CGACCTATCC GTAGCATTAA AAACTTTCTG 
CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512411.1
CDS	3824..12970
Translation	MAEGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514109.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514109.2

ORF Insert Sequence:

ATGGCAGAAG GGTTTCGAAA AAACGATGAA ATGAAAGCTA TGGATGTTTT ACCAATTTTG 
AAGGAAAAAG TTGCATACCT TTCAGGTGGG AGAGATAAAC GTGGAGGTCC CATTTTAACG 
TTTCCGGCCC GCAGCAATCA TGACAGAATA CGACAGGAGG ATCTCAGGAG ACTCATTTCC 
TATCTAGCCT GTATTCCCAG CGAGGAGGTC TGCAAGCGTG GCTTCACGGT GATCGTGGAC 
ATGCGTGGGT CCAAGTGGGA CTCCATCAAG CCCCTTCTGA AGATCCTGCA GGAGTCCTTC 
CCCTGCTGCA TCCATGTGGC CCTGATCATC AAGCCAGACA ACTTCTGGCA GAAACAGAGG 
ACTAATTTTG GCAGTTCTAA ATTTGAATTT GAGACAAATA TGGTCTCTTT AGAAGGCCTT 
ACCAAAGTAG TTGATCCTTC TCAGCTAACT CCTGAGTTTG ATGGCTGCCT GGAATACAAC 
CACGAAGAAT GGATTGAAAT CAGAGTTGCT TTTGAAGACT ACATTAGCAA TGCCACCCAC 
ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT 
TTAGAGGGGG CTCGGAATAT GATCGAGGAA CATTCTCAGC TGAAGAAGAA GGTGATTAAG 
GCCCCCATCG AGGACCTGGA TTTGGAGGGA CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT 
GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA GGCAATGCGG ACCTGCAGAA CCTCTTGCCC 
AAGGTGTCCA CCATGCTGGA CCGGCTGCAC TCGACACGGC AGCATCTGCA CCAGATGTGG 
CATGTGAGGA AGCTGAAGCT GGACCAGTGC TTCCAGCTGA GGCTGTTTGA ACAGGATGCT 
GAGAAGATGT TTGACTGGAT CACACACAAC AAAGGCCTGT TTCTAAACAG CTACACAGAG 
ATTGGGACCA GCCACCCTCA TGCCATGGAG CTTCAGACGC AGCACAATCA CTTTGCCATG 
AACTGTATGA ACGTGTATGT AAATATAAAC CGCATCATGT CGGTGGCCAA TCGTCTGGTG 
GAGTCTGGCC ACTATGCCTC GCAGCAGATC AGGCAGATCG CGAGTCAGCT GGAGCAGGAG 
TGGAAGGCGT TTGCGGCAGC CCTGGATGAG CGGAGCACCT TGCTGGACAT GTCCTCCATT 
TTCCACCAGA AGGCCGAAAA GTATATGAGC AACGTGGATT CATGGTGTAA AGCTTGCGGT 
GAGGTAGACC TTCCCTCAGA GCTGCAGGAC CTAGAAGATG CCATTCATCA CCACCAGGGA 
ATATATGAAC ATATCACTCT TGCTTATTCT GAGGTCAGCC AAGATGGGAA GTCGCTCCTT 
GACAAGCTCC AGCGGCCCTT GACTCCCGGC AGCTCCGATT CCCTGACAGC CTCTGCCAAC 
TACTCCAAGG CCGTGCACCA TGTCCTGGAT GTCATCCACG AGGTGCTGCA CCACCAGCGG 
CAGCTGGAGA ACATCTGGCA ACACCGCAAG GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT 
GTTTTCCAGC AGGACGTTCA GCAGGTGCTA GACTGGATCG AGAACCACGG AGAAGCATTT 
CTGAGCAAAC ATACAGGTGT GGGGAAATCT CTTCATCGGG CCAGAGCATT GCAGAAACGT 
CATGAAGATT TTGAAGAAGT GGCACAGAAC ACATACACCA ATGCGGATAA ATTACTGGAA 
GCAGCAGAAC AGCTGGCTCA GACTGGGGAA TGTGACCCCG AAGAGATTTA TCAGGCTGCC 
CATCAGCTGG AAGACCGGAT TCAAGATTTC GTTCGGCGTG TTGAGCAGCG AAAGATCCTA 
CTGGACATGT CAGTGTCCTT TCACACCCAT GTGAAAGAGC TGTGGACGTG GCTGGAGGAG 
CTGCAGAAGG AGCTGCTGGA CGACGTGTAT GCCGAGTCGG TGGAGGCCGT GCAGGACCTC 
ATCAAGCGCT TTGGCCAGCA GCAGCAGACC ACCCTGCAGG TGACTGTCAA CGTGATCAAG 
GAAGGGGAGG ACCTCATCCA GCAGCTCAGG GACTCTGCCA TCTCCAGTAA CAAGACCCCC 
CACAACAGCT CCATCAACCA CATTGAGACG GTGCTGCAGC AGCTGGACGA GGCGCAGTCG 
CAGATGGAGG AGCTCTTCCA GGAGCGCAAG ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC 
ATCTTCGAGA GGGACGCCAT CGACATTATC TCAGACCTCG AGTCTTGGAA TGATGAGCTT 
TCTCAGCAAA TGAATGACTT CGACACAGAA GATCTCACGA TTGCAGAGCA GCGCCTCCAG 
CACCATGCAG ACAAAGCCTT GACCATGAAC AACTTGACTT TTGACGTCAT CCACCAAGGG 
CAAGATCTTC TGCAGTATGT CAATGAGGTC CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT 
AGAGATGTAG ACATGGCAAC TCGGGTCCAG GACCTGCTGG AGTTTCTTCA TGAAAAACAG 
CAGGAATTGG ATTTAGCCGC AGAGCAGCAT CGGAAACACC TGGAGCAGTG CGTGCAGCTG 
CGCCACCTGC AGGCAGAAGT GAAACAGGTG CTGGGTTGGA TCCGCAACGG AGAGTCCATG 
TTAAATGCCG GACTTATCAC AGCCAGCTCG TTACAAGAGG CAGAGCAGCT CCAGCGAGAG 
CACGAGCAGT TCCAGCATGC CATTGAGAAA ACACATCAGA GCGCGCTGCA GGTGCAGCAG 
AAGGCAGAAG CCATGCTACA GGCCAACCAC TACGACATGG ACATGATCCG GGACTGCGCC 
GAGAAGGTGG CGTCTCACTG GCAACAGCTC ATGCTCAAGA TGGAAGATCG CCTCAAGCTC 
GTCAACGCCT CTGTCGCTTT CTACAAAACC TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC 
CTGGAACAGG AGTACAAGAG AGAAGAAGAC TGGTGTGGCG GGGCGGATAA GCTGGGCCCA 
AACTCTGAGA CGGACCACGT GACGCCCATG ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA 
TTCCTGAAGG CTTGCACCCT TGCTCGGAGG AATGCAGACG TCTTCCTGAA ATACCTGCAC 
AGGAACAGCG TGAACATGCC AGGAATGGTG ACGCACATCA AAGCTCCTGA ACAGCAAGTG 
AAAAATATCT TGAATGAACT CTTCCAACGG GAGAACAGGG TATTGCATTA CTGGACCATG 
AGGAAGAGAC GGCTGGACCA GTGTCAGCAG TACGTGGTCT TTGAGAGGAG TGCCAAGCAG 
GCTTTGGAAT GGATCCATGA CAATGGCGAG TTCTACCTTT CCACACACAC CTCCACGGGC 
TCCAGTATAC AGCACACCCA GGAGCTCCTG AAAGAGCACG AGGAGTTCCA GATAACTGCA 
AAGCAAACCA AAGAGAGAGT GAAGCTATTG ATACAGCTGG CTGATGGCTT TTGTGAAAAA 
GGGCATGCCC ATGCGGCAGA GATAAAAAAA TGTGTTACTG CTGTGGATAA GAGGTACAGA 
GATTTCTCTC TGCGGATGGA GAAGTACAGG ACCTCTTTGG AAAAAGCCCT GGGGATTTCT 
TCAGATTCCA ACAAATCGAG TAAAAGTCTC CAGCTAGATA TCATTCCAGC CAGTATCCCT 
GGCTCAGAGG TGAAACTTCG AGATGCTGCT CATGAACTTA ATGAAGAGAA GCGGAAATCT 
GCCCGCAGGA AAGAGTTCAT AATGGCTGAG CTCATTCAAA CTGAAAAGGC TTATGTAAGA 
GACCTCCGGG AATGTATGGA TACGTACCTG TGGGAAATGA CCAGTGGCGT GGAAGAGATT 
CCACCTGGCA TTGTAAACAA AGAACTCATC ATCTTCGGAA ACATGCAAGA AATCTACGAA 
TTTCATAATA ACATATTCCT AAAGGAGCTG GAAAAATATG AACAGTTGCC AGAGGATGTT 
GGACATTGTT TTGTTACTTG GGCAGACAAG TTTCAGATGT ATGTCACATA TTGCAAAAAT 
AAGCCTGATT CTACTCAGCT GATATTGGAA CATGCAGGGT CCTATTTTGA CGAGATACAG 
CAGCGACATG GATTAGCCAA TTCCATTTCT TCCTACCTTA TTAAACCAGT TCAGCGAATA 
ACGAAGTATC AGCTCCTTTT AAAAGAGCTG CTGACGTGCT GTGAGGAAGG AAAGGGAGAG 
ATTAAAGATG GCCTGGAGGT GATGCTCAGC GTGCCGAAGC GAGCCAATGA TGCCATGCAC 
CTCAGCATGC TGGAAGGGTT TGATGAAAAC ATTGAGTCTC AGGGAGAACT CATCCTACAG 
GAATCCTTCC AAGTGTGGGA CCCAAAAACC TTAATTCGAA AGGGTCGAGA ACGGCATCTC 
TTCCTTTTTG AAATGTCCTT AGTATTTAGT AAAGAAGTGA AAGATTCCAG TGGGAGAAGC 
AAGTACCTTT ATAAAAGCAA ATTGTTTACC TCAGAGTTGG GTGTCACAGA ACATGTTGAA 
GGAGACCCTT GCAAATTTGC ACTGTGGGTG GGGAGAACAC CAACTTCAGA TAATAAAATT 
GTCCTTAAGG CTTCCAGCAT AGAGAACAAG CAGGACTGGA TAAAGCATAT CCGCGAAGTC 
ATCCAGGAGC GGACGATCCA CCTGAAGGGA GCCCTGAAGG AGCCCATTCA CATCCCTAAG 
ACCGCTCCCG CCACAAGACA GAAGGGAAGG AGGGATGGAG AGGATCTGGA CAGCCAAGGA 
GACGGCAGCA GCCAGCCTGA TACGATTTCC ATCGCCTCAC GGACGTCTCA GAACACGCTG 
GACAGCGATA AGCTCTCTGG TGGCTGTGAG CTGACAGTGG TGATCCATGA CTTCACCGCT 
TGCAACAGCA ACGAGCTGAC CATCCGACGG GGCCAGACCG TGGAAGTTCT GGAGCGGCCG 
CATGACAAGC CTGACTGGTG TCTGGTGCGG ACAACTGACC GCTCCCCAGC GGCAGAAGGC 
CTGGTCCCCT GTGGTTCACT GTGCATCGCC CACTCCAGAA GTAGCATGGA AATGGAGGGC 
ATCTTCAACC ACAAAGACTC GCTCTCCGTC TCCAGCAATG ACGCCAGTCC ACCCGCATCC 
GTGGCTTCCC TCCAGCCCCA CATGATCGGG GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG 
GGCAACACCC TGCGCAAGTG GCTCACCAGC CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC 
GACGGGCACG TGAAGAAGCT GGCGCACAAG CACAAGAAGA GCCGCGAGGT CCGCAAGAGC 
GCCGACGCCG GCTCGCAGAA GGACTCCGAC GACAGTGCGG CCACCCCGCA GGACGAGACG 
GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC 
TCGGGGATGC AGAGCTGTGG AGAAGAGGAA GGCGAGGAGG GGGCCGACGC CGTGCCCCTG 
CCGCCACCCA TGGCCATCCA GCAGCACAGC CTCCTCCAGC CAGACTCACA GGATGACAAG 
GCCTCTTCTC GGTTATTAGT CCGCCCCACC AGCTCCGAAA CACCGAGTGC AGCCGAGCTC 
GTCAGTGCAA TTGAGGAACT CGTGAAAAGC AAGATGGCAC TGGAGGATCG CCCCAGCTCA 
CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC CCTTCCTTCA ACCCTTCGGA TAATTCCCTT 
CTCTCTTCCT CCTCGCCCAT TGATGAGATG GAAGAAAGGA AATCCAGCTC TTTAAAGAGA 
AGACACTACG TTTTGCAAGA ACTAGTGGAG ACAGAGCGTG ACTATGTGCG GGACCTTGGC 
TATGTGGTTG AGGGCTACAT GGCACTTATG AAAGAAGATG GTGTTCCTGA TGACATGAAA 
GGAAAAGACA AAATTGTGTT CGGCAACATC CATCAGATTT ACGACTGGCA CAGAGACTTT 
TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA GATCCAGAAA AACTAGGATC CCTTTTTGTT 
AAACACGAGA GAAGGTTGCA CATGTACATA GCTTATTGTC AAAATAAACC AAAGTCTGAG 
CACATTGTCT CAGAATACAT TGATACCTTT TTTGAGGACT TAAAGCAGCG TCTTGGCCAC 
AGGTTACAGC TCACAGATCT GTTGATCAAA CCAGTGCAGA GAATCATGAA GTATCAGCTG 
TTACTGAAGG ACTTCCTCAA GTATTCCAAA AAGGCCAGCC TGGATACATC AGAATTAGAG 
AGAGCTGTGG AAGTCATGTG CATAGTACCC AGGCGGTGCA ACGACATGAT GAACGTGGGG 
CGGCTGCAAG GATTCGACGG GAAAATCGTT GCCCAGGGTA AACTGCTCTT GCAGGACACA 
TTCTTGGTCA CAGACCAAGA TGCAGGACTT CTGCCTCGCT GCAGAGAGAG GCGCATCTTC 
CTCTTTGAGC AGATCGTCAT ATTCAGCGAA CCACTTGATA AAAAGAAGGG CTTCTCCATG 
CCGGGATTCC TGTTTAAGAA CAGTATCAAG GTGAGTTGCC TTTGCCTGGA GGAAAATGTG 
GAAAATGATC CCTGTAAATT TGCTCTGACA TCGAGGACGG GTGACGTGGT AGAGACCTTC 
ATTTTGCATT CATCTAGTCC AAGTGTCCGG CAAACTTGGA TCCATGAAAT CAACCAAATT 
TTAGAAAACC AGCGCAATTT TTTAAATGCC TTGACATCGC CAATCGAGTA CCAGAGGAAC 
CACAGCGGGG GCGGCGGCGG CGGCGGCAGC GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC 
GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC CACAGTGGCG GCCCCAGCAG CTGCGGCGGC 
GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC CGGATCCCCC AGCCTGTCCG ACACCACCCC 
CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC CAGGCAGAGG CAGACAAGAT GTCAGGTACG 
TCCACCCCCG GGCCCTCCCT GCCTCCCCCT GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG 
CCCAGCAGGC GGCCCCCCGG CGCGGACGCC GAGGGGTCCG AGCGAGAAGC GGAGCCGATC 
CCCAAGATGA AGGTGCTGGA GAGCCCCAGG AAAGGCGCCG CGAACGCCTC GGGGTCGAGC 
CCAGACGCCC CCGCCAAGGA CGCGCGCGCT AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC 
CGGGCCGGGG CCGCTTCGCC GCTGAACTCG CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC 
AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG CAGAAGGGGG GCTCCTTCTG GAGCTCCATC 
CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC TTCACCTTCC CGGGGGACAG CGACTCCCTC 
CAGCGGCAGA CACCCCGCCA CGCGGCCCCT GGCAAGGATA CTGACCGCAT GAGCACGTGC 
TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC 
AGTAGCAACA TCTCCACCAT GTTGGTGACA CACGATTACA CGGCAGTGAA GGAGGATGAG 
ATCAACGTCT ACCAAGGAGA GGTCGTTCAA ATTCTGGCCA GCAACCAGCA GAACATGTTT 
CTGGTGTTCC GAGCCGCCAC TGACCAGTGC CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT 
GTCCTGGGCC ACACCAGTGC AGTCATCGTG GAGAACCCGG ACGGGACTCT CAAGAAGTCA 
ACATCTTGGC ACACAGCACT CCGTTTAAGG AAAAAATCTG AGAAAAAAGA TAAAGACGGC 
AAAAGGGAAG GCAAGTTAGA GAACGGTTAT CGGAAGTCAC GGGAAGGACT CAGCAACAAG 
GTATCTGTGA AGCTTCTCAA TCCCAACTAC ATTTATGACG TTCCCCCAGA ATTCGTCATT 
CCATTGAGTG AGGTCACGTG TGAGACAGGG GAGACCGTTG TTCTTAGATG TCGAGTCTGT 
GGCCGCCCCA AAGCCTCAAT TACCTGGAAG GGCCCTGAAC ACAACACCTT GAACAACGAT 
GGTCACTACA GCATCTCCTA CAGTGACCTG GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG 
ACCACGGAAG ATGACGGCAT CTACACGTGC ATCGCTGTCA ATGACATGGG TTCAGCCTCA 
TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA GGGATGGATG GGATCATGGT GACCTGGAAA 
GACAACTTTG ACTCCTTCTA CAGTGAAGTG GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC 
GTTAAGAAAT GTGATCAGAA AGGAACCAAG CGAGCAGTGG CCACTAAGTT TGTGAACAAG 
AAGTTGATGA AGCGCGACCA GGTCACCCAT GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC 
CCCCTGCTTG TCGGCCTCCT CGACACCTTT GAGACCCCCA CCAGCTACAT CCTGGTCTTA 
GAAATGGCTG ACCAGGGTCG CCTCCTGGAC TGCGTGGTGC GATGGGGAAG CCTCACTGAA 
GGGAAGATCA GGGCGCACCT GGGGGAGGTT CTGGAAGCTG TCCGGTACCT GCACAACTGC 
AGGATAGCAC ACCTGGACCT AAAGCCTGAG AATATCCTGG TGGATGAGAG TTTAGCCAAG 
CCAACCATCA AACTGGCTGA CTTTGGAGAT GCTGTTCAGC TCAACACGAC CTACTACATC 
CACCAGTTAC TGGGGAACCC TGAATTCGCA GCCCCTGAAA TCATCCTCGG GAACCCTGTC 
TCCCTGACCT CGGATACGTG GAGTGTTGGA GTGCTCACAT ACGTACTTCT TAGTGGCGTG 
TCCCCCTTCC TGGATGACAG TGTGGAAGAG ACCTGCCTGA ACATTTGCCG CTTAGACTTT 
AGCTTCCCAG ATGACTACTT TAAAGGAGTG AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC 
CTCCTGCAGG AGGACCCCGC CAAGCGTCCC TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG 
CTGCAGGCCG GCAACGGCAG AAGCACGGGC GTCCTCGACA CGTCCAGACT GACTTCCTTC 
ATTGAGCGGC GCAAACACCA GAATGATGTT CGACCTATCC GTAGCATTAA AAACTTTCTG 
CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514110.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512412.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.

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CloneID	OHu61139
Clone ID Related Accession (Same CDS sequence)	XM_011514110.3 , XM_011514110.2
Accession Version	XM_011514110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514110.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512412.1
CDS	313..9429
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514110.3
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514110.3

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_011514110.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512412.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.

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CloneID	OHu61139
Clone ID Related Accession (Same CDS sequence)	XM_011514110.3 , XM_011514110.2
Accession Version	XM_011514110.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512412.1
CDS	312..9428
Translation	MKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_011514110.2
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514110.2

ORF Insert Sequence:

ATGAAAGCTA TGGATGTTTT ACCAATTTTG AAGGAAAAAG TTGCATACCT TTCAGGTGGG 
AGAGATAAAC GTGGAGGTCC CATTTTAACG TTTCCGGCCC GCAGCAATCA TGACAGAATA 
CGACAGGAGG ATCTCAGGAG ACTCATTTCC TATCTAGCCT GTATTCCCAG CGAGGAGGTC 
TGCAAGCGTG GCTTCACGGT GATCGTGGAC ATGCGTGGGT CCAAGTGGGA CTCCATCAAG 
CCCCTTCTGA AGATCCTGCA GGAGTCCTTC CCCTGCTGCA TCCATGTGGC CCTGATCATC 
AAGCCAGACA ACTTCTGGCA GAAACAGAGG ACTAATTTTG GCAGTTCTAA ATTTGAATTT 
GAGACAAATA TGGTCTCTTT AGAAGGCCTT ACCAAAGTAG TTGATCCTTC TCAGCTAACT 
CCTGAGTTTG ATGGCTGCCT GGAATACAAC CACGAAGAAT GGATTGAAAT CAGAGTTGCT 
TTTGAAGACT ACATTAGCAA TGCCACCCAC ATGCTGTCTC GGCTGGAGGA ACTTCAGGAC 
ATCCTAGCTA AGAAGGAGCT GCCTCAGGAT TTAGAGGGGG CTCGGAATAT GATCGAGGAA 
CATTCTCAGC TGAAGAAGAA GGTGATTAAG GCCCCCATCG AGGACCTGGA TTTGGAGGGA 
CAGAAGCTGC TTCAGAGGAT ACAGAGCAGT GAAAGCTTTC CCAAAAAGAA CTCAGGCTCA 
GGCAATGCGG ACCTGCAGAA CCTCTTGCCC AAGGTGTCCA CCATGCTGGA CCGGCTGCAC 
TCGACACGGC AGCATCTGCA CCAGATGTGG CATGTGAGGA AGCTGAAGCT GGACCAGTGC 
TTCCAGCTGA GGCTGTTTGA ACAGGATGCT GAGAAGATGT TTGACTGGAT CACACACAAC 
AAAGGCCTGT TTCTAAACAG CTACACAGAG ATTGGGACCA GCCACCCTCA TGCCATGGAG 
CTTCAGACGC AGCACAATCA CTTTGCCATG AACTGTATGA ACGTGTATGT AAATATAAAC 
CGCATCATGT CGGTGGCCAA TCGTCTGGTG GAGTCTGGCC ACTATGCCTC GCAGCAGATC 
AGGCAGATCG CGAGTCAGCT GGAGCAGGAG TGGAAGGCGT TTGCGGCAGC CCTGGATGAG 
CGGAGCACCT TGCTGGACAT GTCCTCCATT TTCCACCAGA AGGCCGAAAA GTATATGAGC 
AACGTGGATT CATGGTGTAA AGCTTGCGGT GAGGTAGACC TTCCCTCAGA GCTGCAGGAC 
CTAGAAGATG CCATTCATCA CCACCAGGGA ATATATGAAC ATATCACTCT TGCTTATTCT 
GAGGTCAGCC AAGATGGGAA GTCGCTCCTT GACAAGCTCC AGCGGCCCTT GACTCCCGGC 
AGCTCCGATT CCCTGACAGC CTCTGCCAAC TACTCCAAGG CCGTGCACCA TGTCCTGGAT 
GTCATCCACG AGGTGCTGCA CCACCAGCGG CAGCTGGAGA ACATCTGGCA ACACCGCAAG 
GTCCGGCTGC ATCAGAGGCT GCAGCTGTGT GTTTTCCAGC AGGACGTTCA GCAGGTGCTA 
GACTGGATCG AGAACCACGG AGAAGCATTT CTGAGCAAAC ATACAGGTGT GGGGAAATCT 
CTTCATCGGG CCAGAGCATT GCAGAAACGT CATGAAGATT TTGAAGAAGT GGCACAGAAC 
ACATACACCA ATGCGGATAA ATTACTGGAA GCAGCAGAAC AGCTGGCTCA GACTGGGGAA 
TGTGACCCCG AAGAGATTTA TCAGGCTGCC CATCAGCTGG AAGACCGGAT TCAAGATTTC 
GTTCGGCGTG TTGAGCAGCG AAAGATCCTA CTGGACATGT CAGTGTCCTT TCACACCCAT 
GTGAAAGAGC TGTGGACGTG GCTGGAGGAG CTGCAGAAGG AGCTGCTGGA CGACGTGTAT 
GCCGAGTCGG TGGAGGCCGT GCAGGACCTC ATCAAGCGCT TTGGCCAGCA GCAGCAGACC 
ACCCTGCAGG TGACTGTCAA CGTGATCAAG GAAGGGGAGG ACCTCATCCA GCAGCTCAGG 
GACTCTGCCA TCTCCAGTAA CAAGACCCCC CACAACAGCT CCATCAACCA CATTGAGACG 
GTGCTGCAGC AGCTGGACGA GGCGCAGTCG CAGATGGAGG AGCTCTTCCA GGAGCGCAAG 
ATCAAGCTGG AGCTCTTCCT GCAGCTGCGC ATCTTCGAGA GGGACGCCAT CGACATTATC 
TCAGACCTCG AGTCTTGGAA TGATGAGCTT TCTCAGCAAA TGAATGACTT CGACACAGAA 
GATCTCACGA TTGCAGAGCA GCGCCTCCAG CACCATGCAG ACAAAGCCTT GACCATGAAC 
AACTTGACTT TTGACGTCAT CCACCAAGGG CAAGATCTTC TGCAGTATGT CAATGAGGTC 
CAGGCCTCTG GTGTGGAGCT GCTGTGTGAT AGAGATGTAG ACATGGCAAC TCGGGTCCAG 
GACCTGCTGG AGTTTCTTCA TGAAAAACAG CAGGAATTGG ATTTAGCCGC AGAGCAGCAT 
CGGAAACACC TGGAGCAGTG CGTGCAGCTG CGCCACCTGC AGGCAGAAGT GAAACAGGTG 
CTGGGTTGGA TCCGCAACGG AGAGTCCATG TTAAATGCCG GACTTATCAC AGCCAGCTCG 
TTACAAGAGG CAGAGCAGCT CCAGCGAGAG CACGAGCAGT TCCAGCATGC CATTGAGAAA 
ACACATCAGA GCGCGCTGCA GGTGCAGCAG AAGGCAGAAG CCATGCTACA GGCCAACCAC 
TACGACATGG ACATGATCCG GGACTGCGCC GAGAAGGTGG CGTCTCACTG GCAACAGCTC 
ATGCTCAAGA TGGAAGATCG CCTCAAGCTC GTCAACGCCT CTGTCGCTTT CTACAAAACC 
TCAGAGCAGG TCTGCAGCGT CCTCGAGAGC CTGGAACAGG AGTACAAGAG AGAAGAAGAC 
TGGTGTGGCG GGGCGGATAA GCTGGGCCCA AACTCTGAGA CGGACCACGT GACGCCCATG 
ATCAGCAAGC ACCTGGAGCA GAAGGAGGCA TTCCTGAAGG CTTGCACCCT TGCTCGGAGG 
AATGCAGACG TCTTCCTGAA ATACCTGCAC AGGAACAGCG TGAACATGCC AGGAATGGTG 
ACGCACATCA AAGCTCCTGA ACAGCAAGTG AAAAATATCT TGAATGAACT CTTCCAACGG 
GAGAACAGGG TATTGCATTA CTGGACCATG AGGAAGAGAC GGCTGGACCA GTGTCAGCAG 
TACGTGGTCT TTGAGAGGAG TGCCAAGCAG GCTTTGGAAT GGATCCATGA CAATGGCGAG 
TTCTACCTTT CCACACACAC CTCCACGGGC TCCAGTATAC AGCACACCCA GGAGCTCCTG 
AAAGAGCACG AGGAGTTCCA GATAACTGCA AAGCAAACCA AAGAGAGAGT GAAGCTATTG 
ATACAGCTGG CTGATGGCTT TTGTGAAAAA GGGCATGCCC ATGCGGCAGA GATAAAAAAA 
TGTGTTACTG CTGTGGATAA GAGGTACAGA GATTTCTCTC TGCGGATGGA GAAGTACAGG 
ACCTCTTTGG AAAAAGCCCT GGGGATTTCT TCAGATTCCA ACAAATCGAG TAAAAGTCTC 
CAGCTAGATA TCATTCCAGC CAGTATCCCT GGCTCAGAGG TGAAACTTCG AGATGCTGCT 
CATGAACTTA ATGAAGAGAA GCGGAAATCT GCCCGCAGGA AAGAGTTCAT AATGGCTGAG 
CTCATTCAAA CTGAAAAGGC TTATGTAAGA GACCTCCGGG AATGTATGGA TACGTACCTG 
TGGGAAATGA CCAGTGGCGT GGAAGAGATT CCACCTGGCA TTGTAAACAA AGAACTCATC 
ATCTTCGGAA ACATGCAAGA AATCTACGAA TTTCATAATA ACATATTCCT AAAGGAGCTG 
GAAAAATATG AACAGTTGCC AGAGGATGTT GGACATTGTT TTGTTACTTG GGCAGACAAG 
TTTCAGATGT ATGTCACATA TTGCAAAAAT AAGCCTGATT CTACTCAGCT GATATTGGAA 
CATGCAGGGT CCTATTTTGA CGAGATACAG CAGCGACATG GATTAGCCAA TTCCATTTCT 
TCCTACCTTA TTAAACCAGT TCAGCGAATA ACGAAGTATC AGCTCCTTTT AAAAGAGCTG 
CTGACGTGCT GTGAGGAAGG AAAGGGAGAG ATTAAAGATG GCCTGGAGGT GATGCTCAGC 
GTGCCGAAGC GAGCCAATGA TGCCATGCAC CTCAGCATGC TGGAAGGGTT TGATGAAAAC 
ATTGAGTCTC AGGGAGAACT CATCCTACAG GAATCCTTCC AAGTGTGGGA CCCAAAAACC 
TTAATTCGAA AGGGTCGAGA ACGGCATCTC TTCCTTTTTG AAATGTCCTT AGTATTTAGT 
AAAGAAGTGA AAGATTCCAG TGGGAGAAGC AAGTACCTTT ATAAAAGCAA ATTGTTTACC 
TCAGAGTTGG GTGTCACAGA ACATGTTGAA GGAGACCCTT GCAAATTTGC ACTGTGGGTG 
GGGAGAACAC CAACTTCAGA TAATAAAATT GTCCTTAAGG CTTCCAGCAT AGAGAACAAG 
CAGGACTGGA TAAAGCATAT CCGCGAAGTC ATCCAGGAGC GGACGATCCA CCTGAAGGGA 
GCCCTGAAGG AGCCCATTCA CATCCCTAAG ACCGCTCCCG CCACAAGACA GAAGGGAAGG 
AGGGATGGAG AGGATCTGGA CAGCCAAGGA GACGGCAGCA GCCAGCCTGA TACGATTTCC 
ATCGCCTCAC GGACGTCTCA GAACACGCTG GACAGCGATA AGCTCTCTGG TGGCTGTGAG 
CTGACAGTGG TGATCCATGA CTTCACCGCT TGCAACAGCA ACGAGCTGAC CATCCGACGG 
GGCCAGACCG TGGAAGTTCT GGAGCGGCCG CATGACAAGC CTGACTGGTG TCTGGTGCGG 
ACAACTGACC GCTCCCCAGC GGCAGAAGGC CTGGTCCCCT GTGGTTCACT GTGCATCGCC 
CACTCCAGAA GTAGCATGGA AATGGAGGGC ATCTTCAACC ACAAAGACTC GCTCTCCGTC 
TCCAGCAATG ACGCCAGTCC ACCCGCATCC GTGGCTTCCC TCCAGCCCCA CATGATCGGG 
GCCCAGAGCT CGCCGGGCCC CAAGCGGCCG GGCAACACCC TGCGCAAGTG GCTCACCAGC 
CCCGTGCGGC GGCTCAGCAG CGGCAAGGCC GACGGGCACG TGAAGAAGCT GGCGCACAAG 
CACAAGAAGA GCCGCGAGGT CCGCAAGAGC GCCGACGCCG GCTCGCAGAA GGACTCCGAC 
GACAGTGCGG CCACCCCGCA GGACGAGACG GTCGAGGAGA GAGGCCGGAA CGAGGGCCTG 
AGCAGCGGTA CTCTCTCCAA ATCCTCCTCC TCGGGGATGC AGAGCTGTGG AGAAGAGGAA 
GGCGAGGAGG GGGCCGACGC CGTGCCCCTG CCGCCACCCA TGGCCATCCA GCAGCACAGC 
CTCCTCCAGC CAGACTCACA GGATGACAAG GCCTCTTCTC GGTTATTAGT CCGCCCCACC 
AGCTCCGAAA CACCGAGTGC AGCCGAGCTC GTCAGTGCAA TTGAGGAACT CGTGAAAAGC 
AAGATGGCAC TGGAGGATCG CCCCAGCTCA CTCCTTGTTG ACCAGGGAGA TAGTAGCAGC 
CCTTCCTTCA ACCCTTCGGA TAATTCCCTT CTCTCTTCCT CCTCGCCCAT TGATGAGATG 
GAAGAAAGGA AATCCAGCTC TTTAAAGAGA AGACACTACG TTTTGCAAGA ACTAGTGGAG 
ACAGAGCGTG ACTATGTGCG GGACCTTGGC TATGTGGTTG AGGGCTACAT GGCACTTATG 
AAAGAAGATG GTGTTCCTGA TGACATGAAA GGAAAAGACA AAATTGTGTT CGGCAACATC 
CATCAGATTT ACGACTGGCA CAGAGACTTT TTTTTAGGAG AGTTAGAGAA GTGCCTTGAA 
GATCCAGAAA AACTAGGATC CCTTTTTGTT AAACACGAGA GAAGGTTGCA CATGTACATA 
GCTTATTGTC AAAATAAACC AAAGTCTGAG CACATTGTCT CAGAATACAT TGATACCTTT 
TTTGAGGACT TAAAGCAGCG TCTTGGCCAC AGGTTACAGC TCACAGATCT GTTGATCAAA 
CCAGTGCAGA GAATCATGAA GTATCAGCTG TTACTGAAGG ACTTCCTCAA GTATTCCAAA 
AAGGCCAGCC TGGATACATC AGAATTAGAG AGAGCTGTGG AAGTCATGTG CATAGTACCC 
AGGCGGTGCA ACGACATGAT GAACGTGGGG CGGCTGCAAG GATTCGACGG GAAAATCGTT 
GCCCAGGGTA AACTGCTCTT GCAGGACACA TTCTTGGTCA CAGACCAAGA TGCAGGACTT 
CTGCCTCGCT GCAGAGAGAG GCGCATCTTC CTCTTTGAGC AGATCGTCAT ATTCAGCGAA 
CCACTTGATA AAAAGAAGGG CTTCTCCATG CCGGGATTCC TGTTTAAGAA CAGTATCAAG 
GTGAGTTGCC TTTGCCTGGA GGAAAATGTG GAAAATGATC CCTGTAAATT TGCTCTGACA 
TCGAGGACGG GTGACGTGGT AGAGACCTTC ATTTTGCATT CATCTAGTCC AAGTGTCCGG 
CAAACTTGGA TCCATGAAAT CAACCAAATT TTAGAAAACC AGCGCAATTT TTTAAATGCC 
TTGACATCGC CAATCGAGTA CCAGAGGAAC CACAGCGGGG GCGGCGGCGG CGGCGGCAGC 
GGGGGCAGCG GCGGGGGTGG GGGCAGCGGC GGCGGCGGGG CCCCCAGTGG CGGCAGCGGC 
CACAGTGGCG GCCCCAGCAG CTGCGGCGGC GCCCCCAGCA CGAGCAGGAG CCGGCCCTCC 
CGGATCCCCC AGCCTGTCCG ACACCACCCC CCCGTGCTGG TCTCCTCTGC AGCCTCGAGC 
CAGGCAGAGG CAGACAAGAT GTCAGGTACG TCCACCCCCG GGCCCTCCCT GCCTCCCCCT 
GGCGCGGCCC CCGAGGCCGG CCCCAGCGCG CCCAGCAGGC GGCCCCCCGG CGCGGACGCC 
GAGGGGTCCG AGCGAGAAGC GGAGCCGATC CCCAAGATGA AGGTGCTGGA GAGCCCCAGG 
AAAGGCGCCG CGAACGCCTC GGGGTCGAGC CCAGACGCCC CCGCCAAGGA CGCGCGCGCT 
AGCCTGGGCA CCCTGCCGCT TGGGAAGCCC CGGGCCGGGG CCGCTTCGCC GCTGAACTCG 
CCGCTCTCCA GCGCGGTCCC TTCTCTCGGC AAGGAGCCCT TCCCCCCCAG CAGCCCCCTG 
CAGAAGGGGG GCTCCTTCTG GAGCTCCATC CCCGCCTCCC CCGCCAGCCG ACCCGGCTCC 
TTCACCTTCC CGGGGGACAG CGACTCCCTC CAGCGGCAGA CACCCCGCCA CGCGGCCCCT 
GGCAAGGATA CTGACCGCAT GAGCACGTGC TCCTCGGCCA GCGAGCAGTC CGTGCAGTCC 
ACCCAGAGCA ACGGGAGTGA AAGCAGCAGC AGTAGCAACA TCTCCACCAT GTTGGTGACA 
CACGATTACA CGGCAGTGAA GGAGGATGAG ATCAACGTCT ACCAAGGAGA GGTCGTTCAA 
ATTCTGGCCA GCAACCAGCA GAACATGTTT CTGGTGTTCC GAGCCGCCAC TGACCAGTGC 
CCCGCAGCTG AGGGCTGGAT TCCAGGCTTT GTCCTGGGCC ACACCAGTGC AGTCATCGTG 
GAGAACCCGG ACGGGACTCT CAAGAAGTCA ACATCTTGGC ACACAGCACT CCGTTTAAGG 
AAAAAATCTG AGAAAAAAGA TAAAGACGGC AAAAGGGAAG GCAAGTTAGA GAACGGTTAT 
CGGAAGTCAC GGGAAGGACT CAGCAACAAG GTATCTGTGA AGCTTCTCAA TCCCAACTAC 
ATTTATGACG TTCCCCCAGA ATTCGTCATT CCATTGAGTG AGGTCACGTG TGAGACAGGG 
GAGACCGTTG TTCTTAGATG TCGAGTCTGT GGCCGCCCCA AAGCCTCAAT TACCTGGAAG 
GGCCCTGAAC ACAACACCTT GAACAACGAT GGTCACTACA GCATCTCCTA CAGTGACCTG 
GGAGAGGCCA CGCTGAAGAT TGTGGGCGTG ACCACGGAAG ATGACGGCAT CTACACGTGC 
ATCGCTGTCA ATGACATGGG TTCAGCCTCA TCGTCGGCCA GCCTGAGGGT CCTAGGTCCA 
GGGATGGATG GGATCATGGT GACCTGGAAA GACAACTTTG ACTCCTTCTA CAGTGAAGTG 
GCTGAGCTTG GCAGGGGCAG ATTCTCTGTC GTTAAGAAAT GTGATCAGAA AGGAACCAAG 
CGAGCAGTGG CCACTAAGTT TGTGAACAAG AAGTTGATGA AGCGCGACCA GGTCACCCAT 
GAGCTTGGCA TCCTGCAGAG CCTCCAGCAC CCCCTGCTTG TCGGCCTCCT CGACACCTTT 
GAGACCCCCA CCAGCTACAT CCTGGTCTTA GAAATGGCTG ACCAGGGTCG CCTCCTGGAC 
TGCGTGGTGC GATGGGGAAG CCTCACTGAA GGGAAGATCA GGGCGCACCT GGGGGAGGTT 
CTGGAAGCTG TCCGGTACCT GCACAACTGC AGGATAGCAC ACCTGGACCT AAAGCCTGAG 
AATATCCTGG TGGATGAGAG TTTAGCCAAG CCAACCATCA AACTGGCTGA CTTTGGAGAT 
GCTGTTCAGC TCAACACGAC CTACTACATC CACCAGTTAC TGGGGAACCC TGAATTCGCA 
GCCCCTGAAA TCATCCTCGG GAACCCTGTC TCCCTGACCT CGGATACGTG GAGTGTTGGA 
GTGCTCACAT ACGTACTTCT TAGTGGCGTG TCCCCCTTCC TGGATGACAG TGTGGAAGAG 
ACCTGCCTGA ACATTTGCCG CTTAGACTTT AGCTTCCCAG ATGACTACTT TAAAGGAGTG 
AGCCAGAAGG CCAAGGAGTT CGTGTGCTTC CTCCTGCAGG AGGACCCCGC CAAGCGTCCC 
TCGGCTGCGC TGGCCCTCCA GGAGCAGTGG CTGCAGGCCG GCAACGGCAG AAGCACGGGC 
GTCCTCGACA CGTCCAGACT GACTTCCTTC ATTGAGCGGC GCAAACACCA GAATGATGTT 
CGACCTATCC GTAGCATTAA AAACTTTCTG CAGAGCAGGC TTCTGCCTAG AGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_017009801.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865290.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

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CloneID	OHu96041
Clone ID Related Accession (Same CDS sequence)	XM_017009801.1
Accession Version	XM_017009801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGCTT CTCAATCCCA ACTACATTTA TGACGTTCCC 
CCAGAATTCG TCATTCCATT GAGTGAGGTC ACGTGTGAGA CAGGGGAGAC CGTTGTTCTT 
AGATGTCGAG TCTGTGGCCG CCCCAAAGCC TCAATTACCT GGAAGGGCCC TGAACACAAC 
ACCTTGAACA ACGATGGTCA CTACAGCATC TCCTACAGTG ACCTGGGAGA GGCCACGCTG 
AAGATTGTGG GCGTGACCAC GGAAGATGAC GGCATCTACA CGTGCATCGC TGTCAATGAC 
ATGGGTTCAG CCTCATCGTC GGCCAGCCTG AGGGTCCTAG GTCCAGGGAT GGATGGGATC 
ATGGTGACCT GGAAAGACAA CTTTGACTCC TTCTACAGTG AAGTGGCTGA GCTTGGCAGG 
GGCAGATTCT CTGTCGTTAA GAAATGTGAT CAGAAAGGAA CCAAGCGAGC AGTGGCCACT 
AAGTTTGTGA ACAAGAAGTT GATGAAGCGC GACCAGGTCA CCCATGAGCT TGGCATCCTG 
CAGAGCCTCC AGCACCCCCT GCTTGTCGGC CTCCTCGACA CCTTTGAGAC CCCCACCAGC 
TACATCCTGG TCTTAGAAAT GGCTGACCAG GGTCGCCTCC TGGACTGCGT GGTGCGATGG 
GGAAGCCTCA CTGAAGGGAA GATCAGGGCG CACCTGGGGG AGGTTCTGGA AGCTGTCCGG 
TACCTGCACA ACTGCAGGAT AGCACACCTG GACCTAAAGC CTGAGAATAT CCTGGTGGAT 
GAGAGTTTAG CCAAGCCAAC CATCAAACTG GCTGACTTTG GAGATGCTGT TCAGCTCAAC 
ACGACCTACT ACATCCACCA GTTACTGGGG AACCCTGAAT TCGCAGCCCC TGAAATCATC 
CTCGGGAACC CTGTCTCCCT GACCTCGGAT ACGTGGAGTG TTGGAGTGCT CACATACGTA 
CTTCTTAGTG GCGTGTCCCC CTTCCTGGAT GACAGTGTGG AAGAGACCTG CCTGAACATT 
TGCCGCTTAG ACTTTAGCTT CCCAGATGAC TACTTTAAAG GAGTGAGCCA GAAGGCCAAG 
GAGTTCGTGT GCTTCCTCCT GCAGGAGGAC CCCGCCAAGC GTCCCTCGGC TGCGCTGGCC 
CTCCAGGAGC AGTGGCTGCA GGCCGGCAAC GGCAGAAGCA CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAATG ATGTTCGACC TATCCGTAGC 
ATTAAAAACT TTCTGCAGAG CAGGCTTCTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865290.1
CDS	377..9658
Translation	MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_017009801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009801.1

ORF Insert Sequence:

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGCTT CTCAATCCCA ACTACATTTA TGACGTTCCC 
CCAGAATTCG TCATTCCATT GAGTGAGGTC ACGTGTGAGA CAGGGGAGAC CGTTGTTCTT 
AGATGTCGAG TCTGTGGCCG CCCCAAAGCC TCAATTACCT GGAAGGGCCC TGAACACAAC 
ACCTTGAACA ACGATGGTCA CTACAGCATC TCCTACAGTG ACCTGGGAGA GGCCACGCTG 
AAGATTGTGG GCGTGACCAC GGAAGATGAC GGCATCTACA CGTGCATCGC TGTCAATGAC 
ATGGGTTCAG CCTCATCGTC GGCCAGCCTG AGGGTCCTAG GTCCAGGGAT GGATGGGATC 
ATGGTGACCT GGAAAGACAA CTTTGACTCC TTCTACAGTG AAGTGGCTGA GCTTGGCAGG 
GGCAGATTCT CTGTCGTTAA GAAATGTGAT CAGAAAGGAA CCAAGCGAGC AGTGGCCACT 
AAGTTTGTGA ACAAGAAGTT GATGAAGCGC GACCAGGTCA CCCATGAGCT TGGCATCCTG 
CAGAGCCTCC AGCACCCCCT GCTTGTCGGC CTCCTCGACA CCTTTGAGAC CCCCACCAGC 
TACATCCTGG TCTTAGAAAT GGCTGACCAG GGTCGCCTCC TGGACTGCGT GGTGCGATGG 
GGAAGCCTCA CTGAAGGGAA GATCAGGGCG CACCTGGGGG AGGTTCTGGA AGCTGTCCGG 
TACCTGCACA ACTGCAGGAT AGCACACCTG GACCTAAAGC CTGAGAATAT CCTGGTGGAT 
GAGAGTTTAG CCAAGCCAAC CATCAAACTG GCTGACTTTG GAGATGCTGT TCAGCTCAAC 
ACGACCTACT ACATCCACCA GTTACTGGGG AACCCTGAAT TCGCAGCCCC TGAAATCATC 
CTCGGGAACC CTGTCTCCCT GACCTCGGAT ACGTGGAGTG TTGGAGTGCT CACATACGTA 
CTTCTTAGTG GCGTGTCCCC CTTCCTGGAT GACAGTGTGG AAGAGACCTG CCTGAACATT 
TGCCGCTTAG ACTTTAGCTT CCCAGATGAC TACTTTAAAG GAGTGAGCCA GAAGGCCAAG 
GAGTTCGTGT GCTTCCTCCT GCAGGAGGAC CCCGCCAAGC GTCCCTCGGC TGCGCTGGCC 
CTCCAGGAGC AGTGGCTGCA GGCCGGCAAC GGCAGAAGCA CGGGCGTCCT CGACACGTCC 
AGACTGACTT CCTTCATTGA GCGGCGCAAA CACCAGAATG ATGTTCGACC TATCCGTAGC 
ATTAAAAACT TTCTGCAGAG CAGGCTTCTG CCTAGAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_017009802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865291.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X6, mRNA.

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CloneID	OHu96042
Clone ID Related Accession (Same CDS sequence)	XM_017009802.1
Accession Version	XM_017009802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATCCTGAGAA TATCCTGGTG GATGAGAGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865291.1
CDS	377..9019
Translation	MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFETNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDILAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIRQIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDLEDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEILRISWWMRV

Target ORF information:

RefSeq Version	XM_017009802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009802.1

ORF Insert Sequence:

ATGAGCGGCA GCAGCGGCGG AGCCGCCGCC CCCGCCGCGT CCTCCGGCCC CGCCGCGGCG 
GCCAGCGCGG CTGGCTCGGG CTGCGGGGGC GGTGCCGGCG AGGGGGCAGA GGAGGCGGCC 
AAGGACCTGG CCGACATCGC GGCCTTCTTC CGATCCGGGT TTCGAAAAAA CGATGAAATG 
AAAGCTATGG ATGTTTTACC AATTTTGAAG GAAAAAGTTG CATACCTTTC AGGTGGGAGA 
GATAAACGTG GAGGTCCCAT TTTAACGTTT CCGGCCCGCA GCAATCATGA CAGAATACGA 
CAGGAGGATC TCAGGAGACT CATTTCCTAT CTAGCCTGTA TTCCCAGCGA GGAGGTCTGC 
AAGCGTGGCT TCACGGTGAT CGTGGACATG CGTGGGTCCA AGTGGGACTC CATCAAGCCC 
CTTCTGAAGA TCCTGCAGGA GTCCTTCCCC TGCTGCATCC ATGTGGCCCT GATCATCAAG 
CCAGACAACT TCTGGCAGAA ACAGAGGACT AATTTTGGCA GTTCTAAATT TGAATTTGAG 
ACAAATATGG TCTCTTTAGA AGGCCTTACC AAAGTAGTTG ATCCTTCTCA GCTAACTCCT 
GAGTTTGATG GCTGCCTGGA ATACAACCAC GAAGAATGGA TTGAAATCAG AGTTGCTTTT 
GAAGACTACA TTAGCAATGC CACCCACATG CTGTCTCGGC TGGAGGAACT TCAGGACATC 
CTAGCTAAGA AGGAGCTGCC TCAGGATTTA GAGGGGGCTC GGAATATGAT CGAGGAACAT 
TCTCAGCTGA AGAAGAAGGT GATTAAGGCC CCCATCGAGG ACCTGGATTT GGAGGGACAG 
AAGCTGCTTC AGAGGATACA GAGCAGTGAA AGCTTTCCCA AAAAGAACTC AGGCTCAGGC 
AATGCGGACC TGCAGAACCT CTTGCCCAAG GTGTCCACCA TGCTGGACCG GCTGCACTCG 
ACACGGCAGC ATCTGCACCA GATGTGGCAT GTGAGGAAGC TGAAGCTGGA CCAGTGCTTC 
CAGCTGAGGC TGTTTGAACA GGATGCTGAG AAGATGTTTG ACTGGATCAC ACACAACAAA 
GGCCTGTTTC TAAACAGCTA CACAGAGATT GGGACCAGCC ACCCTCATGC CATGGAGCTT 
CAGACGCAGC ACAATCACTT TGCCATGAAC TGTATGAACG TGTATGTAAA TATAAACCGC 
ATCATGTCGG TGGCCAATCG TCTGGTGGAG TCTGGCCACT ATGCCTCGCA GCAGATCAGG 
CAGATCGCGA GTCAGCTGGA GCAGGAGTGG AAGGCGTTTG CGGCAGCCCT GGATGAGCGG 
AGCACCTTGC TGGACATGTC CTCCATTTTC CACCAGAAGG CCGAAAAGTA TATGAGCAAC 
GTGGATTCAT GGTGTAAAGC TTGCGGTGAG GTAGACCTTC CCTCAGAGCT GCAGGACCTA 
GAAGATGCCA TTCATCACCA CCAGGGAATA TATGAACATA TCACTCTTGC TTATTCTGAG 
GTCAGCCAAG ATGGGAAGTC GCTCCTTGAC AAGCTCCAGC GGCCCTTGAC TCCCGGCAGC 
TCCGATTCCC TGACAGCCTC TGCCAACTAC TCCAAGGCCG TGCACCATGT CCTGGATGTC 
ATCCACGAGG TGCTGCACCA CCAGCGGCAG CTGGAGAACA TCTGGCAACA CCGCAAGGTC 
CGGCTGCATC AGAGGCTGCA GCTGTGTGTT TTCCAGCAGG ACGTTCAGCA GGTGCTAGAC 
TGGATCGAGA ACCACGGAGA AGCATTTCTG AGCAAACATA CAGGTGTGGG GAAATCTCTT 
CATCGGGCCA GAGCATTGCA GAAACGTCAT GAAGATTTTG AAGAAGTGGC ACAGAACACA 
TACACCAATG CGGATAAATT ACTGGAAGCA GCAGAACAGC TGGCTCAGAC TGGGGAATGT 
GACCCCGAAG AGATTTATCA GGCTGCCCAT CAGCTGGAAG ACCGGATTCA AGATTTCGTT 
CGGCGTGTTG AGCAGCGAAA GATCCTACTG GACATGTCAG TGTCCTTTCA CACCCATGTG 
AAAGAGCTGT GGACGTGGCT GGAGGAGCTG CAGAAGGAGC TGCTGGACGA CGTGTATGCC 
GAGTCGGTGG AGGCCGTGCA GGACCTCATC AAGCGCTTTG GCCAGCAGCA GCAGACCACC 
CTGCAGGTGA CTGTCAACGT GATCAAGGAA GGGGAGGACC TCATCCAGCA GCTCAGGGAC 
TCTGCCATCT CCAGTAACAA GACCCCCCAC AACAGCTCCA TCAACCACAT TGAGACGGTG 
CTGCAGCAGC TGGACGAGGC GCAGTCGCAG ATGGAGGAGC TCTTCCAGGA GCGCAAGATC 
AAGCTGGAGC TCTTCCTGCA GCTGCGCATC TTCGAGAGGG ACGCCATCGA CATTATCTCA 
GACCTCGAGT CTTGGAATGA TGAGCTTTCT CAGCAAATGA ATGACTTCGA CACAGAAGAT 
CTCACGATTG CAGAGCAGCG CCTCCAGCAC CATGCAGACA AAGCCTTGAC CATGAACAAC 
TTGACTTTTG ACGTCATCCA CCAAGGGCAA GATCTTCTGC AGTATGTCAA TGAGGTCCAG 
GCCTCTGGTG TGGAGCTGCT GTGTGATAGA GATGTAGACA TGGCAACTCG GGTCCAGGAC 
CTGCTGGAGT TTCTTCATGA AAAACAGCAG GAATTGGATT TAGCCGCAGA GCAGCATCGG 
AAACACCTGG AGCAGTGCGT GCAGCTGCGC CACCTGCAGG CAGAAGTGAA ACAGGTGCTG 
GGTTGGATCC GCAACGGAGA GTCCATGTTA AATGCCGGAC TTATCACAGC CAGCTCGTTA 
CAAGAGGCAG AGCAGCTCCA GCGAGAGCAC GAGCAGTTCC AGCATGCCAT TGAGAAAACA 
CATCAGAGCG CGCTGCAGGT GCAGCAGAAG GCAGAAGCCA TGCTACAGGC CAACCACTAC 
GACATGGACA TGATCCGGGA CTGCGCCGAG AAGGTGGCGT CTCACTGGCA ACAGCTCATG 
CTCAAGATGG AAGATCGCCT CAAGCTCGTC AACGCCTCTG TCGCTTTCTA CAAAACCTCA 
GAGCAGGTCT GCAGCGTCCT CGAGAGCCTG GAACAGGAGT ACAAGAGAGA AGAAGACTGG 
TGTGGCGGGG CGGATAAGCT GGGCCCAAAC TCTGAGACGG ACCACGTGAC GCCCATGATC 
AGCAAGCACC TGGAGCAGAA GGAGGCATTC CTGAAGGCTT GCACCCTTGC TCGGAGGAAT 
GCAGACGTCT TCCTGAAATA CCTGCACAGG AACAGCGTGA ACATGCCAGG AATGGTGACG 
CACATCAAAG CTCCTGAACA GCAAGTGAAA AATATCTTGA ATGAACTCTT CCAACGGGAG 
AACAGGGTAT TGCATTACTG GACCATGAGG AAGAGACGGC TGGACCAGTG TCAGCAGTAC 
GTGGTCTTTG AGAGGAGTGC CAAGCAGGCT TTGGAATGGA TCCATGACAA TGGCGAGTTC 
TACCTTTCCA CACACACCTC CACGGGCTCC AGTATACAGC ACACCCAGGA GCTCCTGAAA 
GAGCACGAGG AGTTCCAGAT AACTGCAAAG CAAACCAAAG AGAGAGTGAA GCTATTGATA 
CAGCTGGCTG ATGGCTTTTG TGAAAAAGGG CATGCCCATG CGGCAGAGAT AAAAAAATGT 
GTTACTGCTG TGGATAAGAG GTACAGAGAT TTCTCTCTGC GGATGGAGAA GTACAGGACC 
TCTTTGGAAA AAGCCCTGGG GATTTCTTCA GATTCCAACA AATCGAGTAA AAGTCTCCAG 
CTAGATATCA TTCCAGCCAG TATCCCTGGC TCAGAGGTGA AACTTCGAGA TGCTGCTCAT 
GAACTTAATG AAGAGAAGCG GAAATCTGCC CGCAGGAAAG AGTTCATAAT GGCTGAGCTC 
ATTCAAACTG AAAAGGCTTA TGTAAGAGAC CTCCGGGAAT GTATGGATAC GTACCTGTGG 
GAAATGACCA GTGGCGTGGA AGAGATTCCA CCTGGCATTG TAAACAAAGA ACTCATCATC 
TTCGGAAACA TGCAAGAAAT CTACGAATTT CATAATAACA TATTCCTAAA GGAGCTGGAA 
AAATATGAAC AGTTGCCAGA GGATGTTGGA CATTGTTTTG TTACTTGGGC AGACAAGTTT 
CAGATGTATG TCACATATTG CAAAAATAAG CCTGATTCTA CTCAGCTGAT ATTGGAACAT 
GCAGGGTCCT ATTTTGACGA GATACAGCAG CGACATGGAT TAGCCAATTC CATTTCTTCC 
TACCTTATTA AACCAGTTCA GCGAATAACG AAGTATCAGC TCCTTTTAAA AGAGCTGCTG 
ACGTGCTGTG AGGAAGGAAA GGGAGAGATT AAAGATGGCC TGGAGGTGAT GCTCAGCGTG 
CCGAAGCGAG CCAATGATGC CATGCACCTC AGCATGCTGG AAGGGTTTGA TGAAAACATT 
GAGTCTCAGG GAGAACTCAT CCTACAGGAA TCCTTCCAAG TGTGGGACCC AAAAACCTTA 
ATTCGAAAGG GTCGAGAACG GCATCTCTTC CTTTTTGAAA TGTCCTTAGT ATTTAGTAAA 
GAAGTGAAAG ATTCCAGTGG GAGAAGCAAG TACCTTTATA AAAGCAAATT GTTTACCTCA 
GAGTTGGGTG TCACAGAACA TGTTGAAGGA GACCCTTGCA AATTTGCACT GTGGGTGGGG 
AGAACACCAA CTTCAGATAA TAAAATTGTC CTTAAGGCTT CCAGCATAGA GAACAAGCAG 
GACTGGATAA AGCATATCCG CGAAGTCATC CAGGAGCGGA CGATCCACCT GAAGGGAGCC 
CTGAAGGAGC CCATTCACAT CCCTAAGACC GCTCCCGCCA CAAGACAGAA GGGAAGGAGG 
GATGGAGAGG ATCTGGACAG CCAAGGAGAC GGCAGCAGCC AGCCTGATAC GATTTCCATC 
GCCTCACGGA CGTCTCAGAA CACGCTGGAC AGCGATAAGC TCTCTGGTGG CTGTGAGCTG 
ACAGTGGTGA TCCATGACTT CACCGCTTGC AACAGCAACG AGCTGACCAT CCGACGGGGC 
CAGACCGTGG AAGTTCTGGA GCGGCCGCAT GACAAGCCTG ACTGGTGTCT GGTGCGGACA 
ACTGACCGCT CCCCAGCGGC AGAAGGCCTG GTCCCCTGTG GTTCACTGTG CATCGCCCAC 
TCCAGAAGTA GCATGGAAAT GGAGGGCATC TTCAACCACA AAGACTCGCT CTCCGTCTCC 
AGCAATGACG CCAGTCCACC CGCATCCGTG GCTTCCCTCC AGCCCCACAT GATCGGGGCC 
CAGAGCTCGC CGGGCCCCAA GCGGCCGGGC AACACCCTGC GCAAGTGGCT CACCAGCCCC 
GTGCGGCGGC TCAGCAGCGG CAAGGCCGAC GGGCACGTGA AGAAGCTGGC GCACAAGCAC 
AAGAAGAGCC GCGAGGTCCG CAAGAGCGCC GACGCCGGCT CGCAGAAGGA CTCCGACGAC 
AGTGCGGCCA CCCCGCAGGA CGAGACGGTC GAGGAGAGAG GCCGGAACGA GGGCCTGAGC 
AGCGGTACTC TCTCCAAATC CTCCTCCTCG GGGATGCAGA GCTGTGGAGA AGAGGAAGGC 
GAGGAGGGGG CCGACGCCGT GCCCCTGCCG CCACCCATGG CCATCCAGCA GCACAGCCTC 
CTCCAGCCAG ACTCACAGGA TGACAAGGCC TCTTCTCGGT TATTAGTCCG CCCCACCAGC 
TCCGAAACAC CGAGTGCAGC CGAGCTCGTC AGTGCAATTG AGGAACTCGT GAAAAGCAAG 
ATGGCACTGG AGGATCGCCC CAGCTCACTC CTTGTTGACC AGGGAGATAG TAGCAGCCCT 
TCCTTCAACC CTTCGGATAA TTCCCTTCTC TCTTCCTCCT CGCCCATTGA TGAGATGGAA 
GAAAGGAAAT CCAGCTCTTT AAAGAGAAGA CACTACGTTT TGCAAGAACT AGTGGAGACA 
GAGCGTGACT ATGTGCGGGA CCTTGGCTAT GTGGTTGAGG GCTACATGGC ACTTATGAAA 
GAAGATGGTG TTCCTGATGA CATGAAAGGA AAAGACAAAA TTGTGTTCGG CAACATCCAT 
CAGATTTACG ACTGGCACAG AGACTTTTTT TTAGGAGAGT TAGAGAAGTG CCTTGAAGAT 
CCAGAAAAAC TAGGATCCCT TTTTGTTAAA CACGAGAGAA GGTTGCACAT GTACATAGCT 
TATTGTCAAA ATAAACCAAA GTCTGAGCAC ATTGTCTCAG AATACATTGA TACCTTTTTT 
GAGGACTTAA AGCAGCGTCT TGGCCACAGG TTACAGCTCA CAGATCTGTT GATCAAACCA 
GTGCAGAGAA TCATGAAGTA TCAGCTGTTA CTGAAGGACT TCCTCAAGTA TTCCAAAAAG 
GCCAGCCTGG ATACATCAGA ATTAGAGAGA GCTGTGGAAG TCATGTGCAT AGTACCCAGG 
CGGTGCAACG ACATGATGAA CGTGGGGCGG CTGCAAGGAT TCGACGGGAA AATCGTTGCC 
CAGGGTAAAC TGCTCTTGCA GGACACATTC TTGGTCACAG ACCAAGATGC AGGACTTCTG 
CCTCGCTGCA GAGAGAGGCG CATCTTCCTC TTTGAGCAGA TCGTCATATT CAGCGAACCA 
CTTGATAAAA AGAAGGGCTT CTCCATGCCG GGATTCCTGT TTAAGAACAG TATCAAGGTG 
AGTTGCCTTT GCCTGGAGGA AAATGTGGAA AATGATCCCT GTAAATTTGC TCTGACATCG 
AGGACGGGTG ACGTGGTAGA GACCTTCATT TTGCATTCAT CTAGTCCAAG TGTCCGGCAA 
ACTTGGATCC ATGAAATCAA CCAAATTTTA GAAAACCAGC GCAATTTTTT AAATGCCTTG 
ACATCGCCAA TCGAGTACCA GAGGAACCAC AGCGGGGGCG GCGGCGGCGG CGGCAGCGGG 
GGCAGCGGCG GGGGTGGGGG CAGCGGCGGC GGCGGGGCCC CCAGTGGCGG CAGCGGCCAC 
AGTGGCGGCC CCAGCAGCTG CGGCGGCGCC CCCAGCACGA GCAGGAGCCG GCCCTCCCGG 
ATCCCCCAGC CTGTCCGACA CCACCCCCCC GTGCTGGTCT CCTCTGCAGC CTCGAGCCAG 
GCAGAGGCAG ACAAGATGTC AGGTACGTCC ACCCCCGGGC CCTCCCTGCC TCCCCCTGGC 
GCGGCCCCCG AGGCCGGCCC CAGCGCGCCC AGCAGGCGGC CCCCCGGCGC GGACGCCGAG 
GGGTCCGAGC GAGAAGCGGA GCCGATCCCC AAGATGAAGG TGCTGGAGAG CCCCAGGAAA 
GGCGCCGCGA ACGCCTCGGG GTCGAGCCCA GACGCCCCCG CCAAGGACGC GCGCGCTAGC 
CTGGGCACCC TGCCGCTTGG GAAGCCCCGG GCCGGGGCCG CTTCGCCGCT GAACTCGCCG 
CTCTCCAGCG CGGTCCCTTC TCTCGGCAAG GAGCCCTTCC CCCCCAGCAG CCCCCTGCAG 
AAGGGGGGCT CCTTCTGGAG CTCCATCCCC GCCTCCCCCG CCAGCCGACC CGGCTCCTTC 
ACCTTCCCGG GGGACAGCGA CTCCCTCCAG CGGCAGACAC CCCGCCACGC GGCCCCTGGC 
AAGGATACTG ACCGCATGAG CACGTGCTCC TCGGCCAGCG AGCAGTCCGT GCAGTCCACC 
CAGAGCAACG GGAGTGAAAG CAGCAGCAGT AGCAACATCT CCACCATGTT GGTGACACAC 
GATTACACGG CAGTGAAGGA GGATGAGATC AACGTCTACC AAGGAGAGGT CGTTCAAATT 
CTGGCCAGCA ACCAGCAGAA CATGTTTCTG GTGTTCCGAG CCGCCACTGA CCAGTGCCCC 
GCAGCTGAGG GCTGGATTCC AGGCTTTGTC CTGGGCCACA CCAGTGCAGT CATCGTGGAG 
AACCCGGACG GGACTCTCAA GAAGTCAACA TCTTGGCACA CAGCACTCCG TTTAAGGAAA 
AAATCTGAGA AAAAAGATAA AGACGGCAAA AGGGAAGGCA AGTTAGAGAA CGGTTATCGG 
AAGTCACGGG AAGGACTCAG CAACAAGGTA TCTGTGAAGC TTCTCAATCC CAACTACATT 
TATGACGTTC CCCCAGAATT CGTCATTCCA TTGAGTGAGG TCACGTGTGA GACAGGGGAG 
ACCGTTGTTC TTAGATGTCG AGTCTGTGGC CGCCCCAAAG CCTCAATTAC CTGGAAGGGC 
CCTGAACACA ACACCTTGAA CAACGATGGT CACTACAGCA TCTCCTACAG TGACCTGGGA 
GAGGCCACGC TGAAGATTGT GGGCGTGACC ACGGAAGATG ACGGCATCTA CACGTGCATC 
GCTGTCAATG ACATGGGTTC AGCCTCATCG TCGGCCAGCC TGAGGGTCCT AGGTCCAGGG 
ATGGATGGGA TCATGGTGAC CTGGAAAGAC AACTTTGACT CCTTCTACAG TGAAGTGGCT 
GAGCTTGGCA GGGGCAGATT CTCTGTCGTT AAGAAATGTG ATCAGAAAGG AACCAAGCGA 
GCAGTGGCCA CTAAGTTTGT GAACAAGAAG TTGATGAAGC GCGACCAGGT CACCCATGAG 
CTTGGCATCC TGCAGAGCCT CCAGCACCCC CTGCTTGTCG GCCTCCTCGA CACCTTTGAG 
ACCCCCACCA GCTACATCCT GGTCTTAGAA ATCCTGAGAA TATCCTGGTG GATGAGAGTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIO ( XM_017009803.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865292.1 Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X7, mRNA.

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CloneID	OHu96043
Clone ID Related Accession (Same CDS sequence)	XM_017009803.1
Accession Version	XM_017009803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	triple functional domain protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGT ATCTGTCGTT GGGAACACAC ATGACCTTTC AGGAGGTCAG CCAAGATGGG 
AAGTCGCTCC TTGACAAGCT CCAGCGGCCC TTGACTCCCG GCAGCTCCGA TTCCCTGACA 
GCCTCTGCCA ACTACTCCAA GGCCGTGCAC CATGTCCTGG ATGTCATCCA CGAGGTGCTG 
CACCACCAGC GGCAGCTGGA GAACATCTGG CAACACCGCA AGGTCCGGCT GCATCAGAGG 
CTGCAGCTGT GTGTTTTCCA GCAGGACGTT CAGCAGGTGC TAGACTGGAT CGAGAACCAC 
GGAGAAGCAT TTCTGAGCAA ACATACAGGT GTGGGGAAAT CTCTTCATCG GGCCAGAGCA 
TTGCAGAAAC GTCATGAAGA TTTTGAAGAA GTGGCACAGA ACACATACAC CAATGCGGAT 
AAATTACTGG AAGCAGCAGA ACAGCTGGCT CAGACTGGGG AATGTGACCC CGAAGAGATT 
TATCAGGCTG CCCATCAGCT GGAAGACCGG ATTCAAGATT TCGTTCGGCG TGTTGAGCAG 
CGAAAGATCC TACTGGACAT GTCAGTGTCC TTTCACACCC ATGTGAAAGA GCTGTGGACG 
TGGCTGGAGG AGCTGCAGAA GGAGCTGCTG GACGACGTGT ATGCCGAGTC GGTGGAGGCC 
GTGCAGGACC TCATCAAGCG CTTTGGCCAG CAGCAGCAGA CCACCCTGCA GGTGACTGTC 
AACGTGATCA AGGAAGGGGA GGACCTCATC CAGCAGCTCA GGGACTCTGC CATCTCCAGT 
AACAAGACCC CCCACAACAG CTCCATCAAC CACATTGAGA CGGTGCTGCA GCAGCTGGAC 
GAGGCGCAGT CGCAGATGGA GGAGCTCTTC CAGGAGCGCA AGATCAAGCT GGAGCTCTTC 
CTGCAGCTGC GCATCTTCGA GAGGGACGCC ATCGACATTA TCTCAGACCT CGAGTCTTGG 
AATGATGAGC TTTCTCAGCA AATGAATGAC TTCGACACAG AAGATCTCAC GATTGCAGAG 
CAGCGCCTCC AGCACCATGC AGACAAAGCC TTGACCATGA ACAACTTGAC TTTTGACGTC 
ATCCACCAAG GGCAAGATCT TCTGCAGTAT GTCAATGAGG TCCAGGCCTC TGGTGTGGAG 
CTGCTGTGTG ATAGAGATGT AGACATGGCA ACTCGGGTCC AGGACCTGCT GGAGTTTCTT 
CATGAAAAAC AGCAGGAATT GGATTTAGCC GCAGAGCAGC ATCGGAAACA CCTGGAGCAG 
TGCGTGCAGC TGCGCCACCT GCAGGCAGAA GTGAAACAGG TGCTGGGTTG GATCCGCAAC 
GGAGAGTCCA TGTTAAATGC CGGACTTATC ACAGCCAGCT CGTTACAAGA GGCAGAGCAG 
CTCCAGCGAG AGCACGAGCA GTTCCAGCAT GCCATTGAGA AAACACATCA GAGCGCGCTG 
CAGGTGCAGC AGAAGGCAGA AGCCATGCTA CAGGCCAACC ACTACGACAT GGACATGATC 
CGGGACTGCG CCGAGAAGGT GGCGTCTCAC TGGCAACAGC TCATGCTCAA GATGGAAGAT 
CGCCTCAAGC TCGTCAACGC CTCTGTCGCT TTCTACAAAA CCTCAGAGCA GGTCTGCAGC 
GTCCTCGAGA GCCTGGAACA GGAGTACAAG AGAGAAGAAG ACTGGTGTGG CGGGGCGGAT 
AAGCTGGGCC CAAACTCTGA GACGGACCAC GTGACGCCCA TGATCAGCAA GCACCTGGAG 
CAGAAGGAGG CATTCCTGAA GGCTTGCACC CTTGCTCGGA GGAATGCAGA CGTCTTCCTG 
AAATACCTGC ACAGGAACAG CGTGAACATG CCAGGAATGG TGACGCACAT CAAAGCTCCT 
GAACAGCAAG TGAAAAATAT CTTGAATGAA CTCTTCCAAC GGGAGAACAG GGTATTGCAT 
TACTGGACCA TGAGGAAGAG ACGGCTGGAC CAGTGTCAGC AGTACGTGGT CTTTGAGAGG 
AGTGCCAAGC AGGCTTTGGA ATGGATCCAT GACAATGGCG AGTTCTACCT TTCCACACAC 
ACCTCCACGG GCTCCAGTAT ACAGCACACC CAGGAGCTCC TGAAAGAGCA CGAGGAGTTC 
CAGATAACTG CAAAGCAAAC CAAAGAGAGA GTGAAGCTAT TGATACAGCT GGCTGATGGC 
TTTTGTGAAA AAGGGCATGC CCATGCGGCA GAGATAAAAA AATGTGTTAC TGCTGTGGAT 
AAGAGGTACA GAGATTTCTC TCTGCGGATG GAGAAGTACA GGACCTCTTT GGAAAAAGCC 
CTGGGGATTT CTTCAGATTC CAACAAATCG AGTAAAAGTC TCCAGCTAGA TATCATTCCA 
GCCAGTATCC CTGGCTCAGA GGTGAAACTT CGAGATGCTG CTCATGAACT TAATGAAGAG 
AAGCGGAAAT CTGCCCGCAG GAAAGAGTTC ATAATGGCTG AGCTCATTCA AACTGAAAAG 
GCTTATGTAA GAGACCTCCG GGAATGTATG GATACGTACC TGTGGGAAAT GACCAGTGGC 
GTGGAAGAGA TTCCACCTGG CATTGTAAAC AAAGAACTCA TCATCTTCGG AAACATGCAA 
GAAATCTACG AATTTCATAA TAACATATTC CTAAAGGAGC TGGAAAAATA TGAACAGTTG 
CCAGAGGATG TTGGACATTG TTTTGTTACT TGGGCAGACA AGTTTCAGAT GTATGTCACA 
TATTGCAAAA ATAAGCCTGA TTCTACTCAG CTGATATTGG AACATGCAGG GTCCTATTTT 
GACGAGATAC AGCAGCGACA TGGATTAGCC AATTCCATTT CTTCCTACCT TATTAAACCA 
GTTCAGCGAA TAACGAAGTA TCAGCTCCTT TTAAAAGAGC TGCTGACGTG CTGTGAGGAA 
GGAAAGGGAG AGATTAAAGA TGGCCTGGAG GTGATGCTCA GCGTGCCGAA GCGAGCCAAT 
GATGCCATGC ACCTCAGCAT GCTGGAAGGG TTTGATGAAA ACATTGAGTC TCAGGGAGAA 
CTCATCCTAC AGGAATCCTT CCAAGTGTGG GACCCAAAAA CCTTAATTCG AAAGGGTCGA 
GAACGGCATC TCTTCCTTTT TGAAATGTCC TTAGTATTTA GTAAAGAAGT GAAAGATTCC 
AGTGGGAGAA GCAAGTACCT TTATAAAAGC AAATTGTTTA CCTCAGAGTT GGGTGTCACA 
GAACATGTTG AAGGAGACCC TTGCAAATTT GCACTGTGGG TGGGGAGAAC ACCAACTTCA 
GATAATAAAA TTGTCCTTAA GGCTTCCAGC ATAGAGAACA AGCAGGACTG GATAAAGCAT 
ATCCGCGAAG TCATCCAGGA GCGGACGATC CACCTGAAGG GAGCCCTGAA GGAGCCCATT 
CACATCCCTA AGACCGCTCC CGCCACAAGA CAGAAGGGAA GGAGGGATGG AGAGGATCTG 
GACAGCCAAG GAGACGGCAG CAGCCAGCCT GATACGATTT CCATCGCCTC ACGGACGTCT 
CAGAACACGC TGGACAGCGA TAAGCTCTCT GGTGGCTGTG AGCTGACAGT GGTGATCCAT 
GACTTCACCG CTTGCAACAG CAACGAGCTG ACCATCCGAC GGGGCCAGAC CGTGGAAGTT 
CTGGAGCGGC CGCATGACAA GCCTGACTGG TGTCTGGTGC GGACAACTGA CCGCTCCCCA 
GCGGCAGAAG GCCTGGTCCC CTGTGGTTCA CTGTGCATCG CCCACTCCAG AAGTAGCATG 
GAAATGGAGG GCATCTTCAA CCACAAAGAC TCGCTCTCCG TCTCCAGCAA TGACGCCAGT 
CCACCCGCAT CCGTGGCTTC CCTCCAGCCC CACATGATCG GGGCCCAGAG CTCGCCGGGC 
CCCAAGCGGC CGGGCAACAC CCTGCGCAAG TGGCTCACCA GCCCCGTGCG GCGGCTCAGC 
AGCGGCAAGG CCGACGGGCA CGTGAAGAAG CTGGCGCACA AGCACAAGAA GAGCCGCGAG 
GTCCGCAAGA GCGCCGACGC CGGCTCGCAG AAGGACTCCG ACGACAGTGC GGCCACCCCG 
CAGGACGAGA CGGTCGAGGA GAGAGGCCGG AACGAGGGCC TGAGCAGCGG TACTCTCTCC 
AAATCCTCCT CCTCGGGGAT GCAGAGCTGT GGAGAAGAGG AAGGCGAGGA GGGGGCCGAC 
GCCGTGCCCC TGCCGCCACC CATGGCCATC CAGCAGCACA GCCTCCTCCA GCCAGACTCA 
CAGGATGACA AGGCCTCTTC TCGGTTATTA GTCCGCCCCA CCAGCTCCGA AACACCGAGT 
GCAGCCGAGC TCGTCAGTGC AATTGAGGAA CTCGTGAAAA GCAAGATGGC ACTGGAGGAT 
CGCCCCAGCT CACTCCTTGT TGACCAGGGA GATAGTAGCA GCCCTTCCTT CAACCCTTCG 
GATAATTCCC TTCTCTCTTC CTCCTCGCCC ATTGATGAGA TGGAAGAAAG GAAATCCAGC 
TCTTTAAAGA GAAGACACTA CGTTTTGCAA GAACTAGTGG AGACAGAGCG TGACTATGTG 
CGGGACCTTG GCTATGTGGT TGAGGGCTAC ATGGCACTTA TGAAAGAAGA TGGTGTTCCT 
GATGACATGA AAGGAAAAGA CAAAATTGTG TTCGGCAACA TCCATCAGAT TTACGACTGG 
CACAGAGACT TTTTTTTAGG AGAGTTAGAG AAGTGCCTTG AAGATCCAGA AAAACTAGGA 
TCCCTTTTTG TTAAACACGA GAGAAGGTTG CACATGTACA TAGCTTATTG TCAAAATAAA 
CCAAAGTCTG AGCACATTGT CTCAGAATAC ATTGATACCT TTTTTGAGGA CTTAAAGCAG 
CGTCTTGGCC ACAGGTTACA GCTCACAGAT CTGTTGATCA AACCAGTGCA GAGAATCATG 
AAGTATCAGC TGTTACTGAA GGACTTCCTC AAGTATTCCA AAAAGGCCAG CCTGGATACA 
TCAGAATTAG AGAGAGCTGT GGAAGTCATG TGCATAGTAC CCAGGCGGTG CAACGACATG 
ATGAACGTGG GGCGGCTGCA AGGATTCGAC GGGAAAATCG TTGCCCAGGG TAAACTGCTC 
TTGCAGGACA CATTCTTGGT CACAGACCAA GATGCAGGAC TTCTGCCTCG CTGCAGAGAG 
AGGCGCATCT TCCTCTTTGA GCAGATCGTC ATATTCAGCG AACCACTTGA TAAAAAGAAG 
GGCTTCTCCA TGCCGGGATT CCTGTTTAAG AACAGTATCA AGGTGAGTTG CCTTTGCCTG 
GAGGAAAATG TGGAAAATGA TCCCTGTAAA TTTGCTCTGA CATCGAGGAC GGGTGACGTG 
GTAGAGACCT TCATTTTGCA TTCATCTAGT CCAAGTGTCC GGCAAACTTG GATCCATGAA 
ATCAACCAAA TTTTAGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC GCCAATCGAG 
TACCAGAGGA ACCACAGCGG GGGCGGCGGC GGCGGCGGCA GCGGGGGCAG CGGCGGGGGT 
GGGGGCAGCG GCGGCGGCGG GGCCCCCAGT GGCGGCAGCG GCCACAGTGG CGGCCCCAGC 
AGCTGCGGCG GCGCCCCCAG CACGAGCAGG AGCCGGCCCT CCCGGATCCC CCAGCCTGTC 
CGACACCACC CCCCCGTGCT GGTCTCCTCT GCAGCCTCGA GCCAGGCAGA GGCAGACAAG 
ATGTCAGGTA CGTCCACCCC CGGGCCCTCC CTGCCTCCCC CTGGCGCGGC CCCCGAGGCC 
GGCCCCAGCG CGCCCAGCAG GCGGCCCCCC GGCGCGGACG CCGAGGGGTC CGAGCGAGAA 
GCGGAGCCGA TCCCCAAGAT GAAGGTGCTG GAGAGCCCCA GGAAAGGCGC CGCGAACGCC 
TCGGGGTCGA GCCCAGACGC CCCCGCCAAG GACGCGCGCG CTAGCCTGGG CACCCTGCCG 
CTTGGGAAGC CCCGGGCCGG GGCCGCTTCG CCGCTGAACT CGCCGCTCTC CAGCGCGGTC 
CCTTCTCTCG GCAAGGAGCC CTTCCCCCCC AGCAGCCCCC TGCAGAAGGG GGGCTCCTTC 
TGGAGCTCCA TCCCCGCCTC CCCCGCCAGC CGACCCGGCT CCTTCACCTT CCCGGGGGAC 
AGCGACTCCC TCCAGCGGCA GACACCCCGC CACGCGGCCC CTGGCAAGGA TACTGACCGC 
ATGAGCACGT GCTCCTCGGC CAGCGAGCAG TCCGTGCAGT CCACCCAGAG CAACGGGAGT 
GAAAGCAGCA GCAGTAGCAA CATCTCCACC ATGTTGGTGA CACACGATTA CACGGCAGTG 
AAGGAGGATG AGATCAACGT CTACCAAGGA GAGGTCGTTC AAATTCTGGC CAGCAACCAG 
CAGAACATGT TTCTGGTGTT CCGAGCCGCC ACTGACCAGT GCCCCGCAGC TGAGGGCTGG 
ATTCCAGGCT TTGTCCTGGG CCACACCAGT GCAGTCATCG TGGAGAACCC GGACGGGACT 
CTCAAGAAGT CAACATCTTG GCACACAGCA CTCCGTTTAA GGAAAAAATC TGAGAAAAAA 
GATAAAGACG GCAAAAGGGA AGGCAAGTTA GAGAACGGTT ATCGGAAGTC ACGGGAAGGA 
CTCAGCAACA AGGTATCTGT GAAGCTTCTC AATCCCAACT ACATTTATGA CGTTCCCCCA 
GAATTCGTCA TTCCATTGAG TGAGGTCACG TGTGAGACAG GGGAGACCGT TGTTCTTAGA 
TGTCGAGTCT GTGGCCGCCC CAAAGCCTCA ATTACCTGGA AGGGCCCTGA ACACAACACC 
TTGAACAACG ATGGTCACTA CAGCATCTCC TACAGTGACC TGGGAGAGGC CACGCTGAAG 
ATTGTGGGCG TGACCACGGA AGATGACGGC ATCTACACGT GCATCGCTGT CAATGACATG 
GGTTCAGCCT CATCGTCGGC CAGCCTGAGG GTCCTAGGTC CAGGGATGGA TGGGATCATG 
GTGACCTGGA AAGACAACTT TGACTCCTTC TACAGTGAAG TGGCTGAGCT TGGCAGGGGC 
AGATTCTCTG TCGTTAAGAA ATGTGATCAG AAAGGAACCA AGCGAGCAGT GGCCACTAAG 
TTTGTGAACA AGAAGTTGAT GAAGCGCGAC CAGGTCACCC ATGAGCTTGG CATCCTGCAG 
AGCCTCCAGC ACCCCCTGCT TGTCGGCCTC CTCGACACCT TTGAGACCCC CACCAGCTAC 
ATCCTGGTCT TAGAAATGGC TGACCAGGGT CGCCTCCTGG ACTGCGTGGT GCGATGGGGA 
AGCCTCACTG AAGGGAAGAT CAGGGCGCAC CTGGGGGAGG TTCTGGAAGC TGTCCGGTAC 
CTGCACAACT GCAGGATAGC ACACCTGGAC CTAAAGCCTG AGAATATCCT GGTGGATGAG 
AGTTTAGCCA AGCCAACCAT CAAACTGGCT GACTTTGGAG ATGCTGTTCA GCTCAACACG 
ACCTACTACA TCCACCAGTT ACTGGGGAAC CCTGAATTCG CAGCCCCTGA AATCATCCTC 
GGGAACCCTG TCTCCCTGAC CTCGGATACG TGGAGTGTTG GAGTGCTCAC ATACGTACTT 
CTTAGTGGCG TGTCCCCCTT CCTGGATGAC AGTGTGGAAG AGACCTGCCT GAACATTTGC 
CGCTTAGACT TTAGCTTCCC AGATGACTAC TTTAAAGGAG TGAGCCAGAA GGCCAAGGAG 
TTCGTGTGCT TCCTCCTGCA GGAGGACCCC GCCAAGCGTC CCTCGGCTGC GCTGGCCCTC 
CAGGAGCAGT GGCTGCAGGC CGGCAACGGC AGAAGCACGG GCGTCCTCGA CACGTCCAGA 
CTGACTTCCT TCATTGAGCG GCGCAAACAC CAGAATGATG TTCGACCTAT CCGTAGCATT 
AAAAACTTTC TGCAGAGCAG GCTTCTGCCT AGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865292.1
CDS	101..7939
Translation	MGKYLSLGTHMTFQEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQLDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVGRTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRGQTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVSSNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKHKKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEGEEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVETERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLEDPEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKPVQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVAQGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSRIPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAEGSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSPLSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPGKDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQILASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV

Target ORF information:

RefSeq Version	XM_017009803.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trio Rho guanine nucleotide exchange factor (TRIO), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009803.1

ORF Insert Sequence:

ATGGGAAAGT ATCTGTCGTT GGGAACACAC ATGACCTTTC AGGAGGTCAG CCAAGATGGG 
AAGTCGCTCC TTGACAAGCT CCAGCGGCCC TTGACTCCCG GCAGCTCCGA TTCCCTGACA 
GCCTCTGCCA ACTACTCCAA GGCCGTGCAC CATGTCCTGG ATGTCATCCA CGAGGTGCTG 
CACCACCAGC GGCAGCTGGA GAACATCTGG CAACACCGCA AGGTCCGGCT GCATCAGAGG 
CTGCAGCTGT GTGTTTTCCA GCAGGACGTT CAGCAGGTGC TAGACTGGAT CGAGAACCAC 
GGAGAAGCAT TTCTGAGCAA ACATACAGGT GTGGGGAAAT CTCTTCATCG GGCCAGAGCA 
TTGCAGAAAC GTCATGAAGA TTTTGAAGAA GTGGCACAGA ACACATACAC CAATGCGGAT 
AAATTACTGG AAGCAGCAGA ACAGCTGGCT CAGACTGGGG AATGTGACCC CGAAGAGATT 
TATCAGGCTG CCCATCAGCT GGAAGACCGG ATTCAAGATT TCGTTCGGCG TGTTGAGCAG 
CGAAAGATCC TACTGGACAT GTCAGTGTCC TTTCACACCC ATGTGAAAGA GCTGTGGACG 
TGGCTGGAGG AGCTGCAGAA GGAGCTGCTG GACGACGTGT ATGCCGAGTC GGTGGAGGCC 
GTGCAGGACC TCATCAAGCG CTTTGGCCAG CAGCAGCAGA CCACCCTGCA GGTGACTGTC 
AACGTGATCA AGGAAGGGGA GGACCTCATC CAGCAGCTCA GGGACTCTGC CATCTCCAGT 
AACAAGACCC CCCACAACAG CTCCATCAAC CACATTGAGA CGGTGCTGCA GCAGCTGGAC 
GAGGCGCAGT CGCAGATGGA GGAGCTCTTC CAGGAGCGCA AGATCAAGCT GGAGCTCTTC 
CTGCAGCTGC GCATCTTCGA GAGGGACGCC ATCGACATTA TCTCAGACCT CGAGTCTTGG 
AATGATGAGC TTTCTCAGCA AATGAATGAC TTCGACACAG AAGATCTCAC GATTGCAGAG 
CAGCGCCTCC AGCACCATGC AGACAAAGCC TTGACCATGA ACAACTTGAC TTTTGACGTC 
ATCCACCAAG GGCAAGATCT TCTGCAGTAT GTCAATGAGG TCCAGGCCTC TGGTGTGGAG 
CTGCTGTGTG ATAGAGATGT AGACATGGCA ACTCGGGTCC AGGACCTGCT GGAGTTTCTT 
CATGAAAAAC AGCAGGAATT GGATTTAGCC GCAGAGCAGC ATCGGAAACA CCTGGAGCAG 
TGCGTGCAGC TGCGCCACCT GCAGGCAGAA GTGAAACAGG TGCTGGGTTG GATCCGCAAC 
GGAGAGTCCA TGTTAAATGC CGGACTTATC ACAGCCAGCT CGTTACAAGA GGCAGAGCAG 
CTCCAGCGAG AGCACGAGCA GTTCCAGCAT GCCATTGAGA AAACACATCA GAGCGCGCTG 
CAGGTGCAGC AGAAGGCAGA AGCCATGCTA CAGGCCAACC ACTACGACAT GGACATGATC 
CGGGACTGCG CCGAGAAGGT GGCGTCTCAC TGGCAACAGC TCATGCTCAA GATGGAAGAT 
CGCCTCAAGC TCGTCAACGC CTCTGTCGCT TTCTACAAAA CCTCAGAGCA GGTCTGCAGC 
GTCCTCGAGA GCCTGGAACA GGAGTACAAG AGAGAAGAAG ACTGGTGTGG CGGGGCGGAT 
AAGCTGGGCC CAAACTCTGA GACGGACCAC GTGACGCCCA TGATCAGCAA GCACCTGGAG 
CAGAAGGAGG CATTCCTGAA GGCTTGCACC CTTGCTCGGA GGAATGCAGA CGTCTTCCTG 
AAATACCTGC ACAGGAACAG CGTGAACATG CCAGGAATGG TGACGCACAT CAAAGCTCCT 
GAACAGCAAG TGAAAAATAT CTTGAATGAA CTCTTCCAAC GGGAGAACAG GGTATTGCAT 
TACTGGACCA TGAGGAAGAG ACGGCTGGAC CAGTGTCAGC AGTACGTGGT CTTTGAGAGG 
AGTGCCAAGC AGGCTTTGGA ATGGATCCAT GACAATGGCG AGTTCTACCT TTCCACACAC 
ACCTCCACGG GCTCCAGTAT ACAGCACACC CAGGAGCTCC TGAAAGAGCA CGAGGAGTTC 
CAGATAACTG CAAAGCAAAC CAAAGAGAGA GTGAAGCTAT TGATACAGCT GGCTGATGGC 
TTTTGTGAAA AAGGGCATGC CCATGCGGCA GAGATAAAAA AATGTGTTAC TGCTGTGGAT 
AAGAGGTACA GAGATTTCTC TCTGCGGATG GAGAAGTACA GGACCTCTTT GGAAAAAGCC 
CTGGGGATTT CTTCAGATTC CAACAAATCG AGTAAAAGTC TCCAGCTAGA TATCATTCCA 
GCCAGTATCC CTGGCTCAGA GGTGAAACTT CGAGATGCTG CTCATGAACT TAATGAAGAG 
AAGCGGAAAT CTGCCCGCAG GAAAGAGTTC ATAATGGCTG AGCTCATTCA AACTGAAAAG 
GCTTATGTAA GAGACCTCCG GGAATGTATG GATACGTACC TGTGGGAAAT GACCAGTGGC 
GTGGAAGAGA TTCCACCTGG CATTGTAAAC AAAGAACTCA TCATCTTCGG AAACATGCAA 
GAAATCTACG AATTTCATAA TAACATATTC CTAAAGGAGC TGGAAAAATA TGAACAGTTG 
CCAGAGGATG TTGGACATTG TTTTGTTACT TGGGCAGACA AGTTTCAGAT GTATGTCACA 
TATTGCAAAA ATAAGCCTGA TTCTACTCAG CTGATATTGG AACATGCAGG GTCCTATTTT 
GACGAGATAC AGCAGCGACA TGGATTAGCC AATTCCATTT CTTCCTACCT TATTAAACCA 
GTTCAGCGAA TAACGAAGTA TCAGCTCCTT TTAAAAGAGC TGCTGACGTG CTGTGAGGAA 
GGAAAGGGAG AGATTAAAGA TGGCCTGGAG GTGATGCTCA GCGTGCCGAA GCGAGCCAAT 
GATGCCATGC ACCTCAGCAT GCTGGAAGGG TTTGATGAAA ACATTGAGTC TCAGGGAGAA 
CTCATCCTAC AGGAATCCTT CCAAGTGTGG GACCCAAAAA CCTTAATTCG AAAGGGTCGA 
GAACGGCATC TCTTCCTTTT TGAAATGTCC TTAGTATTTA GTAAAGAAGT GAAAGATTCC 
AGTGGGAGAA GCAAGTACCT TTATAAAAGC AAATTGTTTA CCTCAGAGTT GGGTGTCACA 
GAACATGTTG AAGGAGACCC TTGCAAATTT GCACTGTGGG TGGGGAGAAC ACCAACTTCA 
GATAATAAAA TTGTCCTTAA GGCTTCCAGC ATAGAGAACA AGCAGGACTG GATAAAGCAT 
ATCCGCGAAG TCATCCAGGA GCGGACGATC CACCTGAAGG GAGCCCTGAA GGAGCCCATT 
CACATCCCTA AGACCGCTCC CGCCACAAGA CAGAAGGGAA GGAGGGATGG AGAGGATCTG 
GACAGCCAAG GAGACGGCAG CAGCCAGCCT GATACGATTT CCATCGCCTC ACGGACGTCT 
CAGAACACGC TGGACAGCGA TAAGCTCTCT GGTGGCTGTG AGCTGACAGT GGTGATCCAT 
GACTTCACCG CTTGCAACAG CAACGAGCTG ACCATCCGAC GGGGCCAGAC CGTGGAAGTT 
CTGGAGCGGC CGCATGACAA GCCTGACTGG TGTCTGGTGC GGACAACTGA CCGCTCCCCA 
GCGGCAGAAG GCCTGGTCCC CTGTGGTTCA CTGTGCATCG CCCACTCCAG AAGTAGCATG 
GAAATGGAGG GCATCTTCAA CCACAAAGAC TCGCTCTCCG TCTCCAGCAA TGACGCCAGT 
CCACCCGCAT CCGTGGCTTC CCTCCAGCCC CACATGATCG GGGCCCAGAG CTCGCCGGGC 
CCCAAGCGGC CGGGCAACAC CCTGCGCAAG TGGCTCACCA GCCCCGTGCG GCGGCTCAGC 
AGCGGCAAGG CCGACGGGCA CGTGAAGAAG CTGGCGCACA AGCACAAGAA GAGCCGCGAG 
GTCCGCAAGA GCGCCGACGC CGGCTCGCAG AAGGACTCCG ACGACAGTGC GGCCACCCCG 
CAGGACGAGA CGGTCGAGGA GAGAGGCCGG AACGAGGGCC TGAGCAGCGG TACTCTCTCC 
AAATCCTCCT CCTCGGGGAT GCAGAGCTGT GGAGAAGAGG AAGGCGAGGA GGGGGCCGAC 
GCCGTGCCCC TGCCGCCACC CATGGCCATC CAGCAGCACA GCCTCCTCCA GCCAGACTCA 
CAGGATGACA AGGCCTCTTC TCGGTTATTA GTCCGCCCCA CCAGCTCCGA AACACCGAGT 
GCAGCCGAGC TCGTCAGTGC AATTGAGGAA CTCGTGAAAA GCAAGATGGC ACTGGAGGAT 
CGCCCCAGCT CACTCCTTGT TGACCAGGGA GATAGTAGCA GCCCTTCCTT CAACCCTTCG 
GATAATTCCC TTCTCTCTTC CTCCTCGCCC ATTGATGAGA TGGAAGAAAG GAAATCCAGC 
TCTTTAAAGA GAAGACACTA CGTTTTGCAA GAACTAGTGG AGACAGAGCG TGACTATGTG 
CGGGACCTTG GCTATGTGGT TGAGGGCTAC ATGGCACTTA TGAAAGAAGA TGGTGTTCCT 
GATGACATGA AAGGAAAAGA CAAAATTGTG TTCGGCAACA TCCATCAGAT TTACGACTGG 
CACAGAGACT TTTTTTTAGG AGAGTTAGAG AAGTGCCTTG AAGATCCAGA AAAACTAGGA 
TCCCTTTTTG TTAAACACGA GAGAAGGTTG CACATGTACA TAGCTTATTG TCAAAATAAA 
CCAAAGTCTG AGCACATTGT CTCAGAATAC ATTGATACCT TTTTTGAGGA CTTAAAGCAG 
CGTCTTGGCC ACAGGTTACA GCTCACAGAT CTGTTGATCA AACCAGTGCA GAGAATCATG 
AAGTATCAGC TGTTACTGAA GGACTTCCTC AAGTATTCCA AAAAGGCCAG CCTGGATACA 
TCAGAATTAG AGAGAGCTGT GGAAGTCATG TGCATAGTAC CCAGGCGGTG CAACGACATG 
ATGAACGTGG GGCGGCTGCA AGGATTCGAC GGGAAAATCG TTGCCCAGGG TAAACTGCTC 
TTGCAGGACA CATTCTTGGT CACAGACCAA GATGCAGGAC TTCTGCCTCG CTGCAGAGAG 
AGGCGCATCT TCCTCTTTGA GCAGATCGTC ATATTCAGCG AACCACTTGA TAAAAAGAAG 
GGCTTCTCCA TGCCGGGATT CCTGTTTAAG AACAGTATCA AGGTGAGTTG CCTTTGCCTG 
GAGGAAAATG TGGAAAATGA TCCCTGTAAA TTTGCTCTGA CATCGAGGAC GGGTGACGTG 
GTAGAGACCT TCATTTTGCA TTCATCTAGT CCAAGTGTCC GGCAAACTTG GATCCATGAA 
ATCAACCAAA TTTTAGAAAA CCAGCGCAAT TTTTTAAATG CCTTGACATC GCCAATCGAG 
TACCAGAGGA ACCACAGCGG GGGCGGCGGC GGCGGCGGCA GCGGGGGCAG CGGCGGGGGT 
GGGGGCAGCG GCGGCGGCGG GGCCCCCAGT GGCGGCAGCG GCCACAGTGG CGGCCCCAGC 
AGCTGCGGCG GCGCCCCCAG CACGAGCAGG AGCCGGCCCT CCCGGATCCC CCAGCCTGTC 
CGACACCACC CCCCCGTGCT GGTCTCCTCT GCAGCCTCGA GCCAGGCAGA GGCAGACAAG 
ATGTCAGGTA CGTCCACCCC CGGGCCCTCC CTGCCTCCCC CTGGCGCGGC CCCCGAGGCC 
GGCCCCAGCG CGCCCAGCAG GCGGCCCCCC GGCGCGGACG CCGAGGGGTC CGAGCGAGAA 
GCGGAGCCGA TCCCCAAGAT GAAGGTGCTG GAGAGCCCCA GGAAAGGCGC CGCGAACGCC 
TCGGGGTCGA GCCCAGACGC CCCCGCCAAG GACGCGCGCG CTAGCCTGGG CACCCTGCCG 
CTTGGGAAGC CCCGGGCCGG GGCCGCTTCG CCGCTGAACT CGCCGCTCTC CAGCGCGGTC 
CCTTCTCTCG GCAAGGAGCC CTTCCCCCCC AGCAGCCCCC TGCAGAAGGG GGGCTCCTTC 
TGGAGCTCCA TCCCCGCCTC CCCCGCCAGC CGACCCGGCT CCTTCACCTT CCCGGGGGAC 
AGCGACTCCC TCCAGCGGCA GACACCCCGC CACGCGGCCC CTGGCAAGGA TACTGACCGC 
ATGAGCACGT GCTCCTCGGC CAGCGAGCAG TCCGTGCAGT CCACCCAGAG CAACGGGAGT 
GAAAGCAGCA GCAGTAGCAA CATCTCCACC ATGTTGGTGA CACACGATTA CACGGCAGTG 
AAGGAGGATG AGATCAACGT CTACCAAGGA GAGGTCGTTC AAATTCTGGC CAGCAACCAG 
CAGAACATGT TTCTGGTGTT CCGAGCCGCC ACTGACCAGT GCCCCGCAGC TGAGGGCTGG 
ATTCCAGGCT TTGTCCTGGG CCACACCAGT GCAGTCATCG TGGAGAACCC GGACGGGACT 
CTCAAGAAGT CAACATCTTG GCACACAGCA CTCCGTTTAA GGAAAAAATC TGAGAAAAAA 
GATAAAGACG GCAAAAGGGA AGGCAAGTTA GAGAACGGTT ATCGGAAGTC ACGGGAAGGA 
CTCAGCAACA AGGTATCTGT GAAGCTTCTC AATCCCAACT ACATTTATGA CGTTCCCCCA 
GAATTCGTCA TTCCATTGAG TGAGGTCACG TGTGAGACAG GGGAGACCGT TGTTCTTAGA 
TGTCGAGTCT GTGGCCGCCC CAAAGCCTCA ATTACCTGGA AGGGCCCTGA ACACAACACC 
TTGAACAACG ATGGTCACTA CAGCATCTCC TACAGTGACC TGGGAGAGGC CACGCTGAAG 
ATTGTGGGCG TGACCACGGA AGATGACGGC ATCTACACGT GCATCGCTGT CAATGACATG 
GGTTCAGCCT CATCGTCGGC CAGCCTGAGG GTCCTAGGTC CAGGGATGGA TGGGATCATG 
GTGACCTGGA AAGACAACTT TGACTCCTTC TACAGTGAAG TGGCTGAGCT TGGCAGGGGC 
AGATTCTCTG TCGTTAAGAA ATGTGATCAG AAAGGAACCA AGCGAGCAGT GGCCACTAAG 
TTTGTGAACA AGAAGTTGAT GAAGCGCGAC CAGGTCACCC ATGAGCTTGG CATCCTGCAG 
AGCCTCCAGC ACCCCCTGCT TGTCGGCCTC CTCGACACCT TTGAGACCCC CACCAGCTAC 
ATCCTGGTCT TAGAAATGGC TGACCAGGGT CGCCTCCTGG ACTGCGTGGT GCGATGGGGA 
AGCCTCACTG AAGGGAAGAT CAGGGCGCAC CTGGGGGAGG TTCTGGAAGC TGTCCGGTAC 
CTGCACAACT GCAGGATAGC ACACCTGGAC CTAAAGCCTG AGAATATCCT GGTGGATGAG 
AGTTTAGCCA AGCCAACCAT CAAACTGGCT GACTTTGGAG ATGCTGTTCA GCTCAACACG 
ACCTACTACA TCCACCAGTT ACTGGGGAAC CCTGAATTCG CAGCCCCTGA AATCATCCTC 
GGGAACCCTG TCTCCCTGAC CTCGGATACG TGGAGTGTTG GAGTGCTCAC ATACGTACTT 
CTTAGTGGCG TGTCCCCCTT CCTGGATGAC AGTGTGGAAG AGACCTGCCT GAACATTTGC 
CGCTTAGACT TTAGCTTCCC AGATGACTAC TTTAAAGGAG TGAGCCAGAA GGCCAAGGAG 
TTCGTGTGCT TCCTCCTGCA GGAGGACCCC GCCAAGCGTC CCTCGGCTGC GCTGGCCCTC 
CAGGAGCAGT GGCTGCAGGC CGGCAACGGC AGAAGCACGG GCGTCCTCGA CACGTCCAGA 
CTGACTTCCT TCATTGAGCG GCGCAAACAC CAGAATGATG TTCGACCTAT CCGTAGCATT 
AAAAACTTTC TGCAGAGCAG GCTTCTGCCT AGAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIO
Entrez Gene ID	7204
Full Name	trio Rho guanine nucleotide exchange factor
Synonyms	ARHGEF23,MEBAS,MRD44,tgat
General protein information	Preferred Names trio Rho guanine nucleotide exchange factor Names triple functional domain protein PTPRF-interacting protein triple functional domain (PTPRF interacting)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p15.2
Summary	This gene encodes a large protein that functions as a GDP to GTP exchange factor. This protein promotes the reorganization of the actin cytoskeleton, thereby playing a role in cell migration and growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015].
Disorder MIM:	601893
Disorder Html:	Mental retardation, autosomal dominant 44, 617061 (3)

Homo sapiens (human)	TRIO	NP_009049.2
	TRIO	XP_002804369.1
Canis lupus familiaris (dog)	TRIO	XP_005639720.1
Mus musculus (house mouse)	Trio	NP_001074771.1
Danio rerio (zebrafish)	trioa	NP_001097996.1
Rattus norvegicus (Norway rat)	Trio	XP_003749274.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006107	XP_316167.4
Gallus gallus (chicken)	TRIO	XP_419004.4
Pan troglodytes (chimpanzee)	TRIO	XP_517637.3
Caenorhabditis elegans (roundworm)	unc-73	NP_001021496.1
Xenopus tropicalis (tropical clawed frog)	trio	NP_001123393.1
Drosophila melanogaster (fruit fly)	trio	NP_651960.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIO cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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