TOP3A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP3A
Entrez Gene ID	7156
Full Name	DNA topoisomerase III alpha
Synonyms	TOP3,ZGRF7
General protein information	Preferred Names DNA topoisomerase III alpha Names DNA topoisomerase 3-alpha topo III-alpha topoisomerase (DNA) III alpha zinc finger, GRF-type containing 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p11.2
Summary	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016].
Disorder MIM:	601243

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320759.1	NP_001307688.1	DNA topoisomerase 3-alpha isoform 2
XM_024450903.1	XP_024306671.1	DNA topoisomerase 3-alpha isoform X1
XM_011524001.2	XP_011522303.1	DNA topoisomerase 3-alpha isoform X2
NM_004618.5	NP_004609.1	DNA topoisomerase 3-alpha isoform 1
NM_004618.4	NP_004609.1	DNA topoisomerase 3-alpha isoform 1
NM_001320759.2	NP_001307688.1	DNA topoisomerase 3-alpha isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	top3a	XP_688695.4
Pan troglodytes (chimpanzee)	TOP3A	XP_511323.2
	TOP3A	XP_001095437.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010028	XP_319175.3
Caenorhabditis elegans (roundworm)	top-3	NP_499558.1
Xenopus tropicalis (tropical clawed frog)	top3a	XP_002935889.1
Homo sapiens (human)	TOP3A	NP_004609.1
Bos taurus (cattle)	TOP3A	NP_001071443.1
Mus musculus (house mouse)	Top3a	NP_033436.1
Rattus norvegicus (Norway rat)	Top3a	XP_006246613.1
Gallus gallus (chicken)	TOP3A	NP_001025807.1
Arabidopsis thaliana (thale cress)	TOP3A	NP_201197.1
Canis lupus familiaris (dog)	TOP3A	XP_546656.2
Drosophila melanogaster (fruit fly)	Top3alpha	NP_523602.2
Oryza sativa (rice)	Os03g0165000	NP_001173278.1

Gene Ontology
Bibliography

Related articles in PubMed

Topoisomerase 3? Is Required for Decatenation and Segregation of Human mtDNA.
Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson NG, Larsson E, Falkenberg M, Taylor RW, Griffith JD, Gustafsson CM
Molecular cell69(1)9-23.e6(2018 Jan)

Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III? and RMI1.
Bocquet N, Bizard AH, Abdulrahman W, Larsen NB, Faty M, Cavadini S, Bunker RD, Kowalczykowski SC, Cejka P, Hickson ID, Thom? NH
Nature structural & molecular biology21(3)261-8(2014 Mar)

DNA topoisomerase III alpha regulates p53-mediated tumor suppression.
Hsieh MY, Fan JR, Chang HW, Chen HC, Shen TL, Teng SC, Yeh YH, Li TK
Clinical cancer research : an official journal of the American Association for Cancer Research20(6)1489-501(2014 Mar)

Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.
Daley JM, Chiba T, Xue X, Niu H, Sung P
Nucleic acids research42(17)11083-91(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hTOP3alpha interacts with p53, regulates p53 and p21 expression, and contributes to the p53-mediated tumor suppression
Title: DNA topoisomerase III alpha regulates p53-mediated tumor suppression.

The results show that Topo IIIalpha stimulates DNA unwinding by BLM in a manner that is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalpha-RMI1-RMI2 complex.
Title: Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.

MPS1 protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III alpha-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis
Title: Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III ?-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis.

Study found a correlation between topoisomerase IIIalpha immunoexpression and oral squamous cell carcinoma (OSCC) cancer stem cell immunophenotypes. However, results did not show a prognostic significance of topoisomerases in OSCC>
Title: Topoisomerase expression in oral squamous cell carcinoma: relationship with cancer stem cells profiles and lymph node metastasis.

TOP3A, a new partner gene fused to MLL in an adult patient with de novo acute myeloid leukaemia.
Title: TOP3A, a new partner gene fused to MLL in an adult patient with de novo acute myeloid leukaemia.

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04195	NM_004618.5 Latest version!	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu49926	XM_011524001.2 Latest version!	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu49925	NM_001320759.2 Latest version!	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu104463	XM_024450903.1 Latest version!	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu04195	NM_004618.4	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu49925	NM_001320759.1	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP3A ( NM_004618.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004609.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04195
Clone ID Related Accession (Same CDS sequence)	NM_004618.5 , NM_004618.4
Accession Version	NM_004618.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, BC051748.1, AF086042.1, AC127537.8 and AW294300.1. On Nov 23, 2018 this sequence version replaced NM_004618.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.82081.1, SRR1803611.50754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321105.10/ ENSP00000321636.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC 
GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG 
AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG 
CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG 
TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC 
CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT 
ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG 
ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG 
AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG 
TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT 
TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC 
GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT 
CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC 
AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG 
AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG 
GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT 
GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC 
AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT 
TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC 
GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT 
CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG 
GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG 
CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC 
CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT 
AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG 
GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG 
GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG 
TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG 
TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG 
GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG 
GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT 
AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC 
GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC 
CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC 
CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA 
CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG 
CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA 
GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT 
GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC 
CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC 
CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC 
ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC 
GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG 
GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA 
CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004609.1
CDS	217..3222
Translation	MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	NM_004618.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004618.5

ORF Insert Sequence:

ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC 
GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG 
AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG 
CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG 
TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC 
CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT 
ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG 
ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG 
AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG 
TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT 
TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC 
GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT 
CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC 
AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG 
AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG 
GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT 
GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC 
AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT 
TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC 
GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT 
CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG 
GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG 
CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC 
CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT 
AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG 
GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG 
GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG 
TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG 
TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG 
GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG 
GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT 
AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC 
GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC 
CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC 
CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA 
CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG 
CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA 
GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT 
GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC 
CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC 
CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC 
ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC 
GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG 
GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA 
CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3A ( NM_004618.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004609.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04195
Clone ID Related Accession (Same CDS sequence)	NM_004618.5 , NM_004618.4
Accession Version	NM_004618.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, BC051748.1, AF086042.1, AC127537.8 and AW294300.1. On Mar 4, 2016 this sequence version replaced NM_004618.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.82081.1, SRR1803611.50754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC 
GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG 
AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG 
CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG 
TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC 
CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT 
ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG 
ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG 
AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG 
TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT 
TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC 
GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT 
CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC 
AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG 
AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG 
GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT 
GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC 
AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT 
TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC 
GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT 
CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG 
GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG 
CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC 
CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT 
AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG 
GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG 
GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG 
TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG 
TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG 
GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG 
GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT 
AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC 
GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC 
CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC 
CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA 
CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG 
CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA 
GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT 
GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC 
CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC 
CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC 
ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC 
GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG 
GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA 
CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004609.1
CDS	315..3320
Translation	MIFPVARYALRWLRRPEDRAFSRAAMEMALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLYGQNVTMVMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	NM_004618.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004618.4

ORF Insert Sequence:

ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC 
GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG 
AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG 
CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG 
TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC 
CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT 
ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG 
ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG 
AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG 
TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT 
TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC 
GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT 
CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC 
AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG 
AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG 
GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT 
GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC 
AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT 
TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC 
GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT 
CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG 
GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG 
CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC 
CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT 
AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG 
GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG 
GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG 
TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG 
TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG 
GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG 
GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT 
AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC 
GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC 
CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC 
CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA 
CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG 
CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA 
GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT 
GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC 
CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC 
CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC 
ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC 
GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG 
GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA 
CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3A ( XM_011524001.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522303.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X3, mRNA.

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CloneID	OHu49926
Clone ID Related Accession (Same CDS sequence)	XM_011524001.2
Accession Version	XM_011524001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTGG CCCGAAACTA TCTGGATGTG TATCCATATG ATCACTGGAG TGACAAGATC 
CTCCCTGTCT ATGAGCAAGG ATCCCACTTT CAGCCCAGCA CCGTGGAGAT GGTGGACGGG 
GAGACCAGCC CACCCAAGCT GCTCACCGAG GCCGACCTCA TTGCCCTCAT GGAGAAGCAT 
GGCATTGGTA CGGATGCCAC TCATGCGGAG CACATCGAGA CCATCAAAGC CCGGATGTAC 
GTGGGCCTCA CCCCAGACAA GCGGTTCCTC CCTGGGCACC TGGGCATGGG ACTTGTGGAA 
GGTTATGATT CCATGGGCTA TGAAATGTCT AAGCCTGACC TCCGGGCTGA ACTGGAAGCT 
GATCTGAAGC TGATCTGTGA TGGCAAAAAG GACAAATTTG TGGTTCTAAG GCAGCAAGTG 
CAGAAATACA AGCAGGTTTT CATTGAAGCG GTGGCTAAAG CAAAGAAATT GGACGAGGCC 
TTGGCCCAGT ACTTTGGGAA TGGGACAGAG TTGGCCCAGC AAGAAGATAT CTACCCAGCC 
ATGCCAGAGC CCATCAGGAA GTGCCCACAG TGCAACAAGG ACATGGTCCT TAAGACCAAG 
AAGAATGGCG GGTTCTACCT CAGCTGCATG GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG 
CTTCCTGACT CGGTGCTGGA GGCCAGCAGG GACAGCAGTG TGTGTCCAGT TTGTCAGCCA 
CACCCTGTGT ACAGGTTAAA GTTAAAGTTT AAGCGCGGTA GCCTTCCCCC GACCATGCCT 
CTGGAGTTTG TTTGCTGCAT CGGCGGATGC GACGACACCC TGAGGGAGAT CCTGGACCTG 
AGATTTTCAG GGGGCCCCCC CAGGGCTAGC CAGCCCTCTG GCCGCCTGCA GGCTAACCAG 
TCCCTGAACA GGATGGACAA CAGCCAGCAC CCCCAGCCTG CTGACAGCAG ACAGACTGGG 
TCCTCAAAGG CTCTGGCCCA GACCCTCCCA CCACCCACGG CTGCTGGTGA AAGCAATTCT 
GTGACCTGCA ACTGTGGCCA GGAGGCTGTG CTGCTCACTG TCCGTAAGGA GGGCCCCAAC 
CGGGGCCGGC AGTTCTTTAA GTGCAACGGA GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC 
AGCCCCAATC CGGGAGCAGG AGGGCCTCCT GCCTTGGCAT ATAGACCCCT GGGCGCCTCC 
CTGGGATGCC CACCAGGCCC AGGGATCCAC CTAGGTGGGT TTGGCAACCC TGGTGATGGC 
AGTGGTAGTG GCACATCCTG CCTTTGCAGC CAGCCCTCCG TCACACGGAC TGTGCAGAAG 
GATGGACCCA ACAAGGGGCG CCAGTTCCAC ACATGTGCCA AGCCGAGAGA GCAGCAGTGT 
GGCTTTTTCC AGTGGGTCGA TGAGAACACC GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG 
ACAGGAGACA GAGGAAGAAC CCTGGAGTCG GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT 
TCCTCAGACA TGGGGTCCAC AGCAAAGAAA CCCCGGAAAT GCAGCCTTTG CCACCAGCCT 
GGACACACCC GTCCCTTTTG TCCTCAGAAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522303.1
CDS	816..2411
Translation	MILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	XM_011524001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524001.2

ORF Insert Sequence:

ATGATTCTGG CCCGAAACTA TCTGGATGTG TATCCATATG ATCACTGGAG TGACAAGATC 
CTCCCTGTCT ATGAGCAAGG ATCCCACTTT CAGCCCAGCA CCGTGGAGAT GGTGGACGGG 
GAGACCAGCC CACCCAAGCT GCTCACCGAG GCCGACCTCA TTGCCCTCAT GGAGAAGCAT 
GGCATTGGTA CGGATGCCAC TCATGCGGAG CACATCGAGA CCATCAAAGC CCGGATGTAC 
GTGGGCCTCA CCCCAGACAA GCGGTTCCTC CCTGGGCACC TGGGCATGGG ACTTGTGGAA 
GGTTATGATT CCATGGGCTA TGAAATGTCT AAGCCTGACC TCCGGGCTGA ACTGGAAGCT 
GATCTGAAGC TGATCTGTGA TGGCAAAAAG GACAAATTTG TGGTTCTAAG GCAGCAAGTG 
CAGAAATACA AGCAGGTTTT CATTGAAGCG GTGGCTAAAG CAAAGAAATT GGACGAGGCC 
TTGGCCCAGT ACTTTGGGAA TGGGACAGAG TTGGCCCAGC AAGAAGATAT CTACCCAGCC 
ATGCCAGAGC CCATCAGGAA GTGCCCACAG TGCAACAAGG ACATGGTCCT TAAGACCAAG 
AAGAATGGCG GGTTCTACCT CAGCTGCATG GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG 
CTTCCTGACT CGGTGCTGGA GGCCAGCAGG GACAGCAGTG TGTGTCCAGT TTGTCAGCCA 
CACCCTGTGT ACAGGTTAAA GTTAAAGTTT AAGCGCGGTA GCCTTCCCCC GACCATGCCT 
CTGGAGTTTG TTTGCTGCAT CGGCGGATGC GACGACACCC TGAGGGAGAT CCTGGACCTG 
AGATTTTCAG GGGGCCCCCC CAGGGCTAGC CAGCCCTCTG GCCGCCTGCA GGCTAACCAG 
TCCCTGAACA GGATGGACAA CAGCCAGCAC CCCCAGCCTG CTGACAGCAG ACAGACTGGG 
TCCTCAAAGG CTCTGGCCCA GACCCTCCCA CCACCCACGG CTGCTGGTGA AAGCAATTCT 
GTGACCTGCA ACTGTGGCCA GGAGGCTGTG CTGCTCACTG TCCGTAAGGA GGGCCCCAAC 
CGGGGCCGGC AGTTCTTTAA GTGCAACGGA GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC 
AGCCCCAATC CGGGAGCAGG AGGGCCTCCT GCCTTGGCAT ATAGACCCCT GGGCGCCTCC 
CTGGGATGCC CACCAGGCCC AGGGATCCAC CTAGGTGGGT TTGGCAACCC TGGTGATGGC 
AGTGGTAGTG GCACATCCTG CCTTTGCAGC CAGCCCTCCG TCACACGGAC TGTGCAGAAG 
GATGGACCCA ACAAGGGGCG CCAGTTCCAC ACATGTGCCA AGCCGAGAGA GCAGCAGTGT 
GGCTTTTTCC AGTGGGTCGA TGAGAACACC GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG 
ACAGGAGACA GAGGAAGAAC CCTGGAGTCG GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT 
TCCTCAGACA TGGGGTCCAC AGCAAAGAAA CCCCGGAAAT GCAGCCTTTG CCACCAGCCT 
GGACACACCC GTCCCTTTTG TCCTCAGAAC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3A ( NM_001320759.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307688.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

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CloneID	OHu49925
Clone ID Related Accession (Same CDS sequence)	NM_001320759.1 , NM_001320759.2
Accession Version	NM_001320759.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, AK296437.1, BY996313.1, AF086042.1, AC127537.8 and AW294300.1. On Mar 4, 2016 this sequence version replaced XM_005256776.2. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, resulting in a novel 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.112571.1, SRR1660805.222873.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA 
GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC 
ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA 
GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG 
CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA 
GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA 
GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG 
TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC 
CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT 
TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA 
TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG 
GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC 
ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC 
CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC 
ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT 
CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG 
TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA 
GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA 
AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG 
CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC 
AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT 
GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA 
GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG 
GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC 
TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG 
GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT 
GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC 
AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC 
TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG 
CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG 
TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC 
TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC 
CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG 
GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG 
GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT 
GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC 
TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA 
GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA 
GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA 
TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG 
GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG 
GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA 
AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG 
TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC 
TTTTGTCCTC AGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307688.1
CDS	526..3246
Translation	MNLIIICMARQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	NM_001320759.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320759.1

ORF Insert Sequence:

ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA 
GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC 
ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA 
GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG 
CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA 
GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA 
GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG 
TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC 
CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT 
TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA 
TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG 
GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC 
ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC 
CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC 
ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT 
CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG 
TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA 
GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA 
AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG 
CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC 
AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT 
GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA 
GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG 
GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC 
TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG 
GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT 
GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC 
AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC 
TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG 
CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG 
TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC 
TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC 
CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG 
GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG 
GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT 
GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC 
TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA 
GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA 
GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA 
TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG 
GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG 
GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA 
AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG 
TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC 
TTTTGTCCTC AGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3A ( NM_001320759.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307688.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

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CloneID	OHu49925
Clone ID Related Accession (Same CDS sequence)	NM_001320759.1 , NM_001320759.2
Accession Version	NM_001320759.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, AK296437.1, BY996313.1, AF086042.1, AC127537.8 and AW294300.1. On May 31, 2019 this sequence version replaced NM_001320759.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, resulting in a novel 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.222873.1, SRR1803612.112571.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA 
GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC 
ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA 
GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG 
CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA 
GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA 
GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG 
TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC 
CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT 
TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA 
TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG 
GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC 
ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC 
CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC 
ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT 
CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG 
TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA 
GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA 
AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG 
CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC 
AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT 
GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA 
GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG 
GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC 
TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG 
GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT 
GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC 
AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC 
TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG 
CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG 
TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC 
TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC 
CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG 
GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG 
GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT 
GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC 
TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA 
GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA 
GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA 
TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG 
GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG 
GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA 
AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG 
TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC 
TTTTGTCCTC AGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307688.1
CDS	428..3148
Translation	MNLIIICMARQSCNPLVLFEAEIEKYCPENFVDIKKTLERETRQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGIVEFNWKRHRLFNHTACLVLYQLCVEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	NM_001320759.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320759.2

ORF Insert Sequence:

ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA 
GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC 
ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA 
GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG 
CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA 
GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA 
GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG 
TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC 
CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT 
TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA 
TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG 
GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC 
ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC 
CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC 
ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT 
CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG 
TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA 
GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA 
AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG 
CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC 
AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT 
GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA 
GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG 
GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC 
TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG 
GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT 
GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC 
AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC 
TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG 
CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG 
TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC 
TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC 
CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG 
GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG 
GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT 
GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC 
TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA 
GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA 
GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA 
TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG 
GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG 
GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA 
AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG 
TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC 
TTTTGTCCTC AGAACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP3A ( XM_024450903.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306671.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104463
Clone ID Related Accession (Same CDS sequence)	XM_024450903.1
Accession Version	XM_024450903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA topoisomerase 3-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTG TGGTAGAGGT CAGATCTAAG CCCAAGAGCA AGTGGCGGCC TCAAGCCTTG 
GACACTGTGG AGCTTGAGAA GCTGGCTTCT CGAAAGTTGA GAATAAATGC TAAAGAAACC 
ATGAGGATTG CTGAGAAGCT CTACACTCAA GGGTACATCA GCTATCCCCG AACAGAAACA 
AACATTTTTC CCAGAGACTT AAACCTGACG GTGTTGGTGG AACAGCAGAC CCCCGATCCA 
CGCTGGGGGG CCTTTGCCCA GAGCATTCTA GAGCGGGGTG GTCCCACCCC ACGCAATGGG 
AACAAGTCTG ACCAAGCTCA CCCTCCCATT CACCCCACCA AATACACCAA CAACTTACAG 
GGAGATGAAC AGCGACTGTA CGAGTTTATT GTTCGCCATT TCCTGGCTTG CTGCTCCCAG 
GATGCTCAGG GGCAGGAGAC CACAGTGGAG ATCGACATCG CTCAGGAACG CTTTGTGGCC 
CATGGCCTCA TGATTCTGGC CCGAAACTAT CTGGATGTGT ATCCATATGA TCACTGGAGT 
GACAAGATCC TCCCTGTCTA TGAGCAAGGA TCCCACTTTC AGCCCAGCAC CGTGGAGATG 
GTGGACGGGG AGACCAGCCC ACCCAAGCTG CTCACCGAGG CCGACCTCAT TGCCCTCATG 
GAGAAGCATG GCATTGGTAC GGATGCCACT CATGCGGAGC ACATCGAGAC CATCAAAGCC 
CGGATGTACG TGGGCCTCAC CCCAGACAAG CGGTTCCTCC CTGGGCACCT GGGCATGGGA 
CTTGTGGAAG GTTATGATTC CATGGGCTAT GAAATGTCTA AGCCTGACCT CCGGGCTGAA 
CTGGAAGCTG ATCTGAAGCT GATCTGTGAT GGCAAAAAGG ACAAATTTGT GGTTCTAAGG 
CAGCAAGTGC AGAAATACAA GCAGGTTTTC ATTGAAGCGG TGGCTAAAGC AAAGAAATTG 
GACGAGGCCT TGGCCCAGTA CTTTGGGAAT GGGACAGAGT TGGCCCAGCA AGAAGATATC 
TACCCAGCCA TGCCAGAGCC CATCAGGAAG TGCCCACAGT GCAACAAGGA CATGGTCCTT 
AAGACCAAGA AGAATGGCGG GTTCTACCTC AGCTGCATGG GTTTCCCAGA GTGTCGCTCA 
GCTGTGTGGC TTCCTGACTC GGTGCTGGAG GCCAGCAGGG ACAGCAGTGT GTGTCCAGTT 
TGTCAGCCAC ACCCTGTGTA CAGGTTAAAG TTAAAGTTTA AGCGCGGTAG CCTTCCCCCG 
ACCATGCCTC TGGAGTTTGT TTGCTGCATC GGCGGATGCG ACGACACCCT GAGGGAGATC 
CTGGACCTGA GATTTTCAGG GGGCCCCCCC AGGGCTAGCC AGCCCTCTGG CCGCCTGCAG 
GCTAACCAGT CCCTGAACAG GATGGACAAC AGCCAGCACC CCCAGCCTGC TGACAGCAGA 
CAGACTGGGT CCTCAAAGGC TCTGGCCCAG ACCCTCCCAC CACCCACGGC TGCTGGTGAA 
AGCAATTCTG TGACCTGCAA CTGTGGCCAG GAGGCTGTGC TGCTCACTGT CCGTAAGGAG 
GGCCCCAACC GGGGCCGGCA GTTCTTTAAG TGCAACGGAG GTAGCTGCAA CTTCTTCCTG 
TGGGCAGACA GCCCCAATCC GGGAGCAGGA GGGCCTCCTG CCTTGGCATA TAGACCCCTG 
GGCGCCTCCC TGGGATGCCC ACCAGGCCCA GGGATCCACC TAGGTGGGTT TGGCAACCCT 
GGTGATGGCA GTGGTAGTGG CACATCCTGC CTTTGCAGCC AGCCCTCCGT CACACGGACT 
GTGCAGAAGG ATGGACCCAA CAAGGGGCGC CAGTTCCACA CATGTGCCAA GCCGAGAGAG 
CAGCAGTGTG GCTTTTTCCA GTGGGTCGAT GAGAACACCG CTCCAGGGAC TTCTGGAGCC 
CCGTCCTGGA CAGGAGACAG AGGAAGAACC CTGGAGTCGG AAGCCAGAAG CAAAAGGCCC 
CGGGCCAGTT CCTCAGACAT GGGGTCCACA GCAAAGAAAC CCCGGAAATG CAGCCTTTGC 
CACCAGCCTG GACACACCCG TCCCTTTTGT CCTCAGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306671.1
CDS	1167..3251
Translation	MATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPRDLNLTVLVEQQTPDPRWGAFAQSILERGGPTPRNGNKSDQAHPPIHPTKYTNNLQGDEQRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLMILARNYLDVYPYDHWSDKILPVYEQGSHFQPSTVEMVDGETSPPKLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTPDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICDGKKDKFVVLRQQVQKYKQVFIEAVAKAKKLDEALAQYFGNGTELAQQEDIYPAMPEPIRKCPQCNKDMVLKTKKNGGFYLSCMGFPECRSAVWLPDSVLEASRDSSVCPVCQPHPVYRLKLKFKRGSLPPTMPLEFVCCIGGCDDTLREILDLRFSGGPPRASQPSGRLQANQSLNRMDNSQHPQPADSRQTGSSKALAQTLPPPTAAGESNSVTCNCGQEAVLLTVRKEGPNRGRQFFKCNGGSCNFFLWADSPNPGAGGPPALAYRPLGASLGCPPGPGIHLGGFGNPGDGSGSGTSCLCSQPSVTRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENTAPGTSGAPSWTGDRGRTLESEARSKRPRASSSDMGSTAKKPRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	XM_024450903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450903.1

ORF Insert Sequence:

ATGGCAACTG TGGTAGAGGT CAGATCTAAG CCCAAGAGCA AGTGGCGGCC TCAAGCCTTG 
GACACTGTGG AGCTTGAGAA GCTGGCTTCT CGAAAGTTGA GAATAAATGC TAAAGAAACC 
ATGAGGATTG CTGAGAAGCT CTACACTCAA GGGTACATCA GCTATCCCCG AACAGAAACA 
AACATTTTTC CCAGAGACTT AAACCTGACG GTGTTGGTGG AACAGCAGAC CCCCGATCCA 
CGCTGGGGGG CCTTTGCCCA GAGCATTCTA GAGCGGGGTG GTCCCACCCC ACGCAATGGG 
AACAAGTCTG ACCAAGCTCA CCCTCCCATT CACCCCACCA AATACACCAA CAACTTACAG 
GGAGATGAAC AGCGACTGTA CGAGTTTATT GTTCGCCATT TCCTGGCTTG CTGCTCCCAG 
GATGCTCAGG GGCAGGAGAC CACAGTGGAG ATCGACATCG CTCAGGAACG CTTTGTGGCC 
CATGGCCTCA TGATTCTGGC CCGAAACTAT CTGGATGTGT ATCCATATGA TCACTGGAGT 
GACAAGATCC TCCCTGTCTA TGAGCAAGGA TCCCACTTTC AGCCCAGCAC CGTGGAGATG 
GTGGACGGGG AGACCAGCCC ACCCAAGCTG CTCACCGAGG CCGACCTCAT TGCCCTCATG 
GAGAAGCATG GCATTGGTAC GGATGCCACT CATGCGGAGC ACATCGAGAC CATCAAAGCC 
CGGATGTACG TGGGCCTCAC CCCAGACAAG CGGTTCCTCC CTGGGCACCT GGGCATGGGA 
CTTGTGGAAG GTTATGATTC CATGGGCTAT GAAATGTCTA AGCCTGACCT CCGGGCTGAA 
CTGGAAGCTG ATCTGAAGCT GATCTGTGAT GGCAAAAAGG ACAAATTTGT GGTTCTAAGG 
CAGCAAGTGC AGAAATACAA GCAGGTTTTC ATTGAAGCGG TGGCTAAAGC AAAGAAATTG 
GACGAGGCCT TGGCCCAGTA CTTTGGGAAT GGGACAGAGT TGGCCCAGCA AGAAGATATC 
TACCCAGCCA TGCCAGAGCC CATCAGGAAG TGCCCACAGT GCAACAAGGA CATGGTCCTT 
AAGACCAAGA AGAATGGCGG GTTCTACCTC AGCTGCATGG GTTTCCCAGA GTGTCGCTCA 
GCTGTGTGGC TTCCTGACTC GGTGCTGGAG GCCAGCAGGG ACAGCAGTGT GTGTCCAGTT 
TGTCAGCCAC ACCCTGTGTA CAGGTTAAAG TTAAAGTTTA AGCGCGGTAG CCTTCCCCCG 
ACCATGCCTC TGGAGTTTGT TTGCTGCATC GGCGGATGCG ACGACACCCT GAGGGAGATC 
CTGGACCTGA GATTTTCAGG GGGCCCCCCC AGGGCTAGCC AGCCCTCTGG CCGCCTGCAG 
GCTAACCAGT CCCTGAACAG GATGGACAAC AGCCAGCACC CCCAGCCTGC TGACAGCAGA 
CAGACTGGGT CCTCAAAGGC TCTGGCCCAG ACCCTCCCAC CACCCACGGC TGCTGGTGAA 
AGCAATTCTG TGACCTGCAA CTGTGGCCAG GAGGCTGTGC TGCTCACTGT CCGTAAGGAG 
GGCCCCAACC GGGGCCGGCA GTTCTTTAAG TGCAACGGAG GTAGCTGCAA CTTCTTCCTG 
TGGGCAGACA GCCCCAATCC GGGAGCAGGA GGGCCTCCTG CCTTGGCATA TAGACCCCTG 
GGCGCCTCCC TGGGATGCCC ACCAGGCCCA GGGATCCACC TAGGTGGGTT TGGCAACCCT 
GGTGATGGCA GTGGTAGTGG CACATCCTGC CTTTGCAGCC AGCCCTCCGT CACACGGACT 
GTGCAGAAGG ATGGACCCAA CAAGGGGCGC CAGTTCCACA CATGTGCCAA GCCGAGAGAG 
CAGCAGTGTG GCTTTTTCCA GTGGGTCGAT GAGAACACCG CTCCAGGGAC TTCTGGAGCC 
CCGTCCTGGA CAGGAGACAG AGGAAGAACC CTGGAGTCGG AAGCCAGAAG CAAAAGGCCC 
CGGGCCAGTT CCTCAGACAT GGGGTCCACA GCAAAGAAAC CCCGGAAATG CAGCCTTTGC 
CACCAGCCTG GACACACCCG TCCCTTTTGT CCTCAGAACA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Topoisomerase 3? Is Required for Decatenation and Segregation of Human mtDNA.
Molecular cell69(1)9-23.e6(2018 Jan)
Nicholls TJ,Nadalutti CA,Motori E,Sommerville EW,Gorman GS,Basu S,Hoberg E,Turnbull DM,Chinnery PF,Larsson NG,Larsson E,Falkenberg M,Taylor RW,Griffith JD,Gustafsson CM

Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III? and RMI1.
Nature structural & molecular biology21(3)261-8(2014 Mar)
Bocquet N,Bizard AH,Abdulrahman W,Larsen NB,Faty M,Cavadini S,Bunker RD,Kowalczykowski SC,Cejka P,Hickson ID,Thom? NH

DNA topoisomerase III alpha regulates p53-mediated tumor suppression.
Clinical cancer research : an official journal of the American Association for Cancer Research20(6)1489-501(2014 Mar)
Hsieh MY,Fan JR,Chang HW,Chen HC,Shen TL,Teng SC,Yeh YH,Li TK

Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.
Nucleic acids research42(17)11083-91(2014)
Daley JM,Chiba T,Xue X,Niu H,Sung P

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TOP3A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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