TOP3A cDNA ORF clone, Homo sapiens(human)

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu04195 NM_004618.5
Latest version!
Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu49926 XM_011524001.2
Latest version!
Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu49925 NM_001320759.2
Latest version!
Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu104463 XM_024450903.1
Latest version!
Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu04195 NM_004618.4 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$399.50-$559.30
$799.00
OHu49925 NM_001320759.1 Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu04195
    Clone ID Related Accession (Same CDS sequence) NM_004618.5 , NM_004618.4
    Accession Version NM_004618.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, BC051748.1, AF086042.1, AC127537.8 and AW294300.1. On Nov 23, 2018 this sequence version replaced NM_004618.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.82081.1, SRR1803611.50754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321105.10/ ENSP00000321636.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
    TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC
    GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG
    AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG
    CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC
    CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT
    ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG
    ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG
    AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG
    TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT
    TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG
    GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC
    GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT
    CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC
    AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC
    AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG
    GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC
    AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC
    GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG
    TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT
    CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG
    CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC
    CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT
    AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG
    GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG
    GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG
    TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG
    TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG
    GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG
    GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT
    AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC
    GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC
    CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC
    CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA
    CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG
    CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA
    GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT
    GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC
    CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC
    CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC
    ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC
    GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG
    GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA
    CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004609.1
    CDS217..3222
    Translation

    Target ORF information:

    RefSeq Version NM_004618.5
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004618.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
    TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC
    GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG
    AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG
    CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC
    CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT
    ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG
    ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG
    AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG
    TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT
    TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG
    GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC
    GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT
    CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC
    AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC
    AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG
    GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC
    AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC
    GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG
    TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT
    CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG
    CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC
    CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT
    AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG
    GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG
    GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG
    TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG
    TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG
    GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG
    GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT
    AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC
    GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC
    CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC
    CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA
    CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG
    CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA
    GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT
    GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC
    CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC
    CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC
    ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC
    GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG
    GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA
    CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu04195
    Clone ID Related Accession (Same CDS sequence) NM_004618.5 , NM_004618.4
    Accession Version NM_004618.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3006bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, BC051748.1, AF086042.1, AC127537.8 and AW294300.1. On Mar 4, 2016 this sequence version replaced NM_004618.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.82081.1, SRR1803611.50754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
    TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC
    GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG
    AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG
    CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC
    CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT
    ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG
    ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG
    AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG
    TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT
    TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG
    GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC
    GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT
    CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC
    AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC
    AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG
    GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC
    AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC
    GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG
    TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT
    CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG
    CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC
    CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT
    AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG
    GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG
    GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG
    TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG
    TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG
    GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG
    GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT
    AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC
    GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC
    CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC
    CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA
    CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG
    CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA
    GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT
    GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC
    CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC
    CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC
    ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC
    GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG
    GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA
    CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004609.1
    CDS315..3320
    Translation

    Target ORF information:

    RefSeq Version NM_004618.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004618.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGATCTTTC CTGTCGCCCG CTACGCGCTC CGGTGGCTGC GACGGCCCGA AGACCGTGCC 
    TTTTCCCGCG CCGCCATGGA GATGGCCCTC CGAGGCGTGC GGAAAGTCCT CTGTGTGGCC
    GAAAAAAACG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CAAACGGTCG CATGAGGCGG
    AGAGAAGGAC TTTCAAAATT CAACAAGATC TATGAATTTG ATTATCATCT GTATGGCCAG
    AATGTTACCA TGGTAATGAC TTCAGTTTCT GGACATTTAC TGGCTCATGA TTTCCAGATG
    CAGTTTCGAA AATGGCAGAG CTGCAACCCT CTTGTCCTCT TTGAAGCAGA AATTGAAAAG
    TACTGCCCAG AGAATTTTGT AGACATCAAG AAAACTTTGG AACGAGAGAC TCGCCAGTGC
    CAGGCTCTGG TGATCTGGAC TGACTGTGAT AGAGAAGGCG AAAACATCGG GTTTGAGATT
    ATCCACGTGT GTAAGGCTGT AAAGCCCAAT CTGCAGGTGT TGCGAGCCCG ATTCTCTGAG
    ATCACACCCC ATGCCGTCAG GACAGCTTGT GAAAACCTGA CCGAGCCTGA TCAGAGGGTG
    AGCGATGCTG TGGATGTGAG GCAGGAGCTG GACCTGAGGA TTGGAGCTGC CTTTACTAGG
    TTCCAGACCC TGCGGCTTCA GAGGATTTTT CCTGAGGTGC TGGCAGAGCA GCTCATCAGT
    TACGGCAGCT GCCAGTTCCC CACACTGGGC TTTGTGGTGG AGCGGTTCAA AGCCATTCAG
    GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTATC
    GTAGAATTCA ACTGGAAAAG GCATCGACTC TTTAACCACA CGGCTTGCCT AGTTCTCTAT
    CAGTTGTGTG TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAGATCTAA GCCCAAGAGC
    AAGTGGCGGC CTCAAGCCTT GGACACTGTG GAGCTTGAGA AGCTGGCTTC TCGAAAGTTG
    AGAATAAATG CTAAAGAAAC CATGAGGATT GCTGAGAAGC TCTACACTCA AGGGTACATC
    AGCTATCCCC GAACAGAAAC AAACATTTTT CCCAGAGACT TAAACCTGAC GGTGTTGGTG
    GAACAGCAGA CCCCCGATCC ACGCTGGGGG GCCTTTGCCC AGAGCATTCT AGAGCGGGGT
    GGTCCCACCC CACGCAATGG GAACAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACC
    AAATACACCA ACAACTTACA GGGAGATGAA CAGCGACTGT ACGAGTTTAT TGTTCGCCAT
    TTCCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAGA CCACAGTGGA GATCGACATC
    GCTCAGGAAC GCTTTGTGGC CCATGGCCTC ATGATTCTGG CCCGAAACTA TCTGGATGTG
    TATCCATATG ATCACTGGAG TGACAAGATC CTCCCTGTCT ATGAGCAAGG ATCCCACTTT
    CAGCCCAGCA CCGTGGAGAT GGTGGACGGG GAGACCAGCC CACCCAAGCT GCTCACCGAG
    GCCGACCTCA TTGCCCTCAT GGAGAAGCAT GGCATTGGTA CGGATGCCAC TCATGCGGAG
    CACATCGAGA CCATCAAAGC CCGGATGTAC GTGGGCCTCA CCCCAGACAA GCGGTTCCTC
    CCTGGGCACC TGGGCATGGG ACTTGTGGAA GGTTATGATT CCATGGGCTA TGAAATGTCT
    AAGCCTGACC TCCGGGCTGA ACTGGAAGCT GATCTGAAGC TGATCTGTGA TGGCAAAAAG
    GACAAATTTG TGGTTCTAAG GCAGCAAGTG CAGAAATACA AGCAGGTTTT CATTGAAGCG
    GTGGCTAAAG CAAAGAAATT GGACGAGGCC TTGGCCCAGT ACTTTGGGAA TGGGACAGAG
    TTGGCCCAGC AAGAAGATAT CTACCCAGCC ATGCCAGAGC CCATCAGGAA GTGCCCACAG
    TGCAACAAGG ACATGGTCCT TAAGACCAAG AAGAATGGCG GGTTCTACCT CAGCTGCATG
    GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG CTTCCTGACT CGGTGCTGGA GGCCAGCAGG
    GACAGCAGTG TGTGTCCAGT TTGTCAGCCA CACCCTGTGT ACAGGTTAAA GTTAAAGTTT
    AAGCGCGGTA GCCTTCCCCC GACCATGCCT CTGGAGTTTG TTTGCTGCAT CGGCGGATGC
    GACGACACCC TGAGGGAGAT CCTGGACCTG AGATTTTCAG GGGGCCCCCC CAGGGCTAGC
    CAGCCCTCTG GCCGCCTGCA GGCTAACCAG TCCCTGAACA GGATGGACAA CAGCCAGCAC
    CCCCAGCCTG CTGACAGCAG ACAGACTGGG TCCTCAAAGG CTCTGGCCCA GACCCTCCCA
    CCACCCACGG CTGCTGGTGA AAGCAATTCT GTGACCTGCA ACTGTGGCCA GGAGGCTGTG
    CTGCTCACTG TCCGTAAGGA GGGCCCCAAC CGGGGCCGGC AGTTCTTTAA GTGCAACGGA
    GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC AGCCCCAATC CGGGAGCAGG AGGGCCTCCT
    GCCTTGGCAT ATAGACCCCT GGGCGCCTCC CTGGGATGCC CACCAGGCCC AGGGATCCAC
    CTAGGTGGGT TTGGCAACCC TGGTGATGGC AGTGGTAGTG GCACATCCTG CCTTTGCAGC
    CAGCCCTCCG TCACACGGAC TGTGCAGAAG GATGGACCCA ACAAGGGGCG CCAGTTCCAC
    ACATGTGCCA AGCCGAGAGA GCAGCAGTGT GGCTTTTTCC AGTGGGTCGA TGAGAACACC
    GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG ACAGGAGACA GAGGAAGAAC CCTGGAGTCG
    GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT TCCTCAGACA TGGGGTCCAC AGCAAAGAAA
    CCCCGGAAAT GCAGCCTTTG CCACCAGCCT GGACACACCC GTCCCTTTTG TCCTCAGAAC
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49926
    Clone ID Related Accession (Same CDS sequence) XM_011524001.2
    Accession Version XM_011524001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATTCTGG CCCGAAACTA TCTGGATGTG TATCCATATG ATCACTGGAG TGACAAGATC 
    CTCCCTGTCT ATGAGCAAGG ATCCCACTTT CAGCCCAGCA CCGTGGAGAT GGTGGACGGG
    GAGACCAGCC CACCCAAGCT GCTCACCGAG GCCGACCTCA TTGCCCTCAT GGAGAAGCAT
    GGCATTGGTA CGGATGCCAC TCATGCGGAG CACATCGAGA CCATCAAAGC CCGGATGTAC
    GTGGGCCTCA CCCCAGACAA GCGGTTCCTC CCTGGGCACC TGGGCATGGG ACTTGTGGAA
    GGTTATGATT CCATGGGCTA TGAAATGTCT AAGCCTGACC TCCGGGCTGA ACTGGAAGCT
    GATCTGAAGC TGATCTGTGA TGGCAAAAAG GACAAATTTG TGGTTCTAAG GCAGCAAGTG
    CAGAAATACA AGCAGGTTTT CATTGAAGCG GTGGCTAAAG CAAAGAAATT GGACGAGGCC
    TTGGCCCAGT ACTTTGGGAA TGGGACAGAG TTGGCCCAGC AAGAAGATAT CTACCCAGCC
    ATGCCAGAGC CCATCAGGAA GTGCCCACAG TGCAACAAGG ACATGGTCCT TAAGACCAAG
    AAGAATGGCG GGTTCTACCT CAGCTGCATG GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG
    CTTCCTGACT CGGTGCTGGA GGCCAGCAGG GACAGCAGTG TGTGTCCAGT TTGTCAGCCA
    CACCCTGTGT ACAGGTTAAA GTTAAAGTTT AAGCGCGGTA GCCTTCCCCC GACCATGCCT
    CTGGAGTTTG TTTGCTGCAT CGGCGGATGC GACGACACCC TGAGGGAGAT CCTGGACCTG
    AGATTTTCAG GGGGCCCCCC CAGGGCTAGC CAGCCCTCTG GCCGCCTGCA GGCTAACCAG
    TCCCTGAACA GGATGGACAA CAGCCAGCAC CCCCAGCCTG CTGACAGCAG ACAGACTGGG
    TCCTCAAAGG CTCTGGCCCA GACCCTCCCA CCACCCACGG CTGCTGGTGA AAGCAATTCT
    GTGACCTGCA ACTGTGGCCA GGAGGCTGTG CTGCTCACTG TCCGTAAGGA GGGCCCCAAC
    CGGGGCCGGC AGTTCTTTAA GTGCAACGGA GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC
    AGCCCCAATC CGGGAGCAGG AGGGCCTCCT GCCTTGGCAT ATAGACCCCT GGGCGCCTCC
    CTGGGATGCC CACCAGGCCC AGGGATCCAC CTAGGTGGGT TTGGCAACCC TGGTGATGGC
    AGTGGTAGTG GCACATCCTG CCTTTGCAGC CAGCCCTCCG TCACACGGAC TGTGCAGAAG
    GATGGACCCA ACAAGGGGCG CCAGTTCCAC ACATGTGCCA AGCCGAGAGA GCAGCAGTGT
    GGCTTTTTCC AGTGGGTCGA TGAGAACACC GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG
    ACAGGAGACA GAGGAAGAAC CCTGGAGTCG GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT
    TCCTCAGACA TGGGGTCCAC AGCAAAGAAA CCCCGGAAAT GCAGCCTTTG CCACCAGCCT
    GGACACACCC GTCCCTTTTG TCCTCAGAAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522303.1
    CDS816..2411
    Translation

    Target ORF information:

    RefSeq Version XM_011524001.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATTCTGG CCCGAAACTA TCTGGATGTG TATCCATATG ATCACTGGAG TGACAAGATC 
    CTCCCTGTCT ATGAGCAAGG ATCCCACTTT CAGCCCAGCA CCGTGGAGAT GGTGGACGGG
    GAGACCAGCC CACCCAAGCT GCTCACCGAG GCCGACCTCA TTGCCCTCAT GGAGAAGCAT
    GGCATTGGTA CGGATGCCAC TCATGCGGAG CACATCGAGA CCATCAAAGC CCGGATGTAC
    GTGGGCCTCA CCCCAGACAA GCGGTTCCTC CCTGGGCACC TGGGCATGGG ACTTGTGGAA
    GGTTATGATT CCATGGGCTA TGAAATGTCT AAGCCTGACC TCCGGGCTGA ACTGGAAGCT
    GATCTGAAGC TGATCTGTGA TGGCAAAAAG GACAAATTTG TGGTTCTAAG GCAGCAAGTG
    CAGAAATACA AGCAGGTTTT CATTGAAGCG GTGGCTAAAG CAAAGAAATT GGACGAGGCC
    TTGGCCCAGT ACTTTGGGAA TGGGACAGAG TTGGCCCAGC AAGAAGATAT CTACCCAGCC
    ATGCCAGAGC CCATCAGGAA GTGCCCACAG TGCAACAAGG ACATGGTCCT TAAGACCAAG
    AAGAATGGCG GGTTCTACCT CAGCTGCATG GGTTTCCCAG AGTGTCGCTC AGCTGTGTGG
    CTTCCTGACT CGGTGCTGGA GGCCAGCAGG GACAGCAGTG TGTGTCCAGT TTGTCAGCCA
    CACCCTGTGT ACAGGTTAAA GTTAAAGTTT AAGCGCGGTA GCCTTCCCCC GACCATGCCT
    CTGGAGTTTG TTTGCTGCAT CGGCGGATGC GACGACACCC TGAGGGAGAT CCTGGACCTG
    AGATTTTCAG GGGGCCCCCC CAGGGCTAGC CAGCCCTCTG GCCGCCTGCA GGCTAACCAG
    TCCCTGAACA GGATGGACAA CAGCCAGCAC CCCCAGCCTG CTGACAGCAG ACAGACTGGG
    TCCTCAAAGG CTCTGGCCCA GACCCTCCCA CCACCCACGG CTGCTGGTGA AAGCAATTCT
    GTGACCTGCA ACTGTGGCCA GGAGGCTGTG CTGCTCACTG TCCGTAAGGA GGGCCCCAAC
    CGGGGCCGGC AGTTCTTTAA GTGCAACGGA GGTAGCTGCA ACTTCTTCCT GTGGGCAGAC
    AGCCCCAATC CGGGAGCAGG AGGGCCTCCT GCCTTGGCAT ATAGACCCCT GGGCGCCTCC
    CTGGGATGCC CACCAGGCCC AGGGATCCAC CTAGGTGGGT TTGGCAACCC TGGTGATGGC
    AGTGGTAGTG GCACATCCTG CCTTTGCAGC CAGCCCTCCG TCACACGGAC TGTGCAGAAG
    GATGGACCCA ACAAGGGGCG CCAGTTCCAC ACATGTGCCA AGCCGAGAGA GCAGCAGTGT
    GGCTTTTTCC AGTGGGTCGA TGAGAACACC GCTCCAGGGA CTTCTGGAGC CCCGTCCTGG
    ACAGGAGACA GAGGAAGAAC CCTGGAGTCG GAAGCCAGAA GCAAAAGGCC CCGGGCCAGT
    TCCTCAGACA TGGGGTCCAC AGCAAAGAAA CCCCGGAAAT GCAGCCTTTG CCACCAGCCT
    GGACACACCC GTCCCTTTTG TCCTCAGAAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49925
    Clone ID Related Accession (Same CDS sequence) NM_001320759.1 , NM_001320759.2
    Accession Version NM_001320759.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, AK296437.1, BY996313.1, AF086042.1, AC127537.8 and AW294300.1. On Mar 4, 2016 this sequence version replaced XM_005256776.2. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, resulting in a novel 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.112571.1, SRR1660805.222873.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA
    GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC
    ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA
    GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG
    CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA
    GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA
    GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG
    TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC
    CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT
    TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA
    TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG
    GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC
    ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC
    CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC
    ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT
    CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG
    TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA
    GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA
    AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG
    CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC
    AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT
    GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA
    GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG
    GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC
    TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG
    GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT
    GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC
    AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC
    TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG
    CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG
    TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC
    TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC
    CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG
    GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG
    GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT
    GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC
    TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA
    GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA
    GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA
    TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG
    GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG
    GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA
    AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG
    TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC
    TTTTGTCCTC AGAACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307688.1
    CDS526..3246
    Translation

    Target ORF information:

    RefSeq Version NM_001320759.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001320759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA
    GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC
    ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA
    GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG
    CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA
    GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA
    GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG
    TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC
    CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT
    TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA
    TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG
    GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC
    ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC
    CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC
    ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT
    CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG
    TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA
    GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA
    AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG
    CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC
    AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT
    GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA
    GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG
    GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC
    TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG
    GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT
    GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC
    AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC
    TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG
    CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG
    TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC
    TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC
    CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG
    GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG
    GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT
    GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC
    TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA
    GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA
    GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA
    TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG
    GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG
    GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA
    AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG
    TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC
    TTTTGTCCTC AGAACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49925
    Clone ID Related Accession (Same CDS sequence) NM_001320759.1 , NM_001320759.2
    Accession Version NM_001320759.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM462184.1, AK296437.1, BY996313.1, AF086042.1, AC127537.8 and AW294300.1. On May 31, 2019 this sequence version replaced NM_001320759.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, resulting in a novel 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.222873.1, SRR1803612.112571.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA
    GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC
    ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA
    GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG
    CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA
    GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA
    GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG
    TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC
    CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT
    TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA
    TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG
    GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC
    ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC
    CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC
    ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT
    CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG
    TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA
    GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA
    AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG
    CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC
    AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT
    GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA
    GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG
    GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC
    TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG
    GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT
    GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC
    AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC
    TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG
    CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG
    TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC
    TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC
    CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG
    GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG
    GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT
    GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC
    TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA
    GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA
    GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA
    TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG
    GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG
    GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA
    AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG
    TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC
    TTTTGTCCTC AGAACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307688.1
    CDS428..3148
    Translation

    Target ORF information:

    RefSeq Version NM_001320759.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001320759.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAATTTGA TTATCATCTG TATGGCCAGG CAGAGCTGCA ACCCTCTTGT CCTCTTTGAA 
    GCAGAAATTG AAAAGTACTG CCCAGAGAAT TTTGTAGACA TCAAGAAAAC TTTGGAACGA
    GAGACTCGCC AGTGCCAGGC TCTGGTGATC TGGACTGACT GTGATAGAGA AGGCGAAAAC
    ATCGGGTTTG AGATTATCCA CGTGTGTAAG GCTGTAAAGC CCAATCTGCA GGTGTTGCGA
    GCCCGATTCT CTGAGATCAC ACCCCATGCC GTCAGGACAG CTTGTGAAAA CCTGACCGAG
    CCTGATCAGA GGGTGAGCGA TGCTGTGGAT GTGAGGCAGG AGCTGGACCT GAGGATTGGA
    GCTGCCTTTA CTAGGTTCCA GACCCTGCGG CTTCAGAGGA TTTTTCCTGA GGTGCTGGCA
    GAGCAGCTCA TCAGTTACGG CAGCTGCCAG TTCCCCACAC TGGGCTTTGT GGTGGAGCGG
    TTCAAAGCCA TTCAGGCTTT TGTACCAGAA ATCTTCCACA GAATTAAAGT AACTCATGAC
    CACAAAGATG GTATCGTAGA ATTCAACTGG AAAAGGCATC GACTCTTTAA CCACACGGCT
    TGCCTAGTTC TCTATCAGTT GTGTGTGGAG GATCCCATGG CAACTGTGGT AGAGGTCAGA
    TCTAAGCCCA AGAGCAAGTG GCGGCCTCAA GCCTTGGACA CTGTGGAGCT TGAGAAGCTG
    GCTTCTCGAA AGTTGAGAAT AAATGCTAAA GAAACCATGA GGATTGCTGA GAAGCTCTAC
    ACTCAAGGGT ACATCAGCTA TCCCCGAACA GAAACAAACA TTTTTCCCAG AGACTTAAAC
    CTGACGGTGT TGGTGGAACA GCAGACCCCC GATCCACGCT GGGGGGCCTT TGCCCAGAGC
    ATTCTAGAGC GGGGTGGTCC CACCCCACGC AATGGGAACA AGTCTGACCA AGCTCACCCT
    CCCATTCACC CCACCAAATA CACCAACAAC TTACAGGGAG ATGAACAGCG ACTGTACGAG
    TTTATTGTTC GCCATTTCCT GGCTTGCTGC TCCCAGGATG CTCAGGGGCA GGAGACCACA
    GTGGAGATCG ACATCGCTCA GGAACGCTTT GTGGCCCATG GCCTCATGAT TCTGGCCCGA
    AACTATCTGG ATGTGTATCC ATATGATCAC TGGAGTGACA AGATCCTCCC TGTCTATGAG
    CAAGGATCCC ACTTTCAGCC CAGCACCGTG GAGATGGTGG ACGGGGAGAC CAGCCCACCC
    AAGCTGCTCA CCGAGGCCGA CCTCATTGCC CTCATGGAGA AGCATGGCAT TGGTACGGAT
    GCCACTCATG CGGAGCACAT CGAGACCATC AAAGCCCGGA TGTACGTGGG CCTCACCCCA
    GACAAGCGGT TCCTCCCTGG GCACCTGGGC ATGGGACTTG TGGAAGGTTA TGATTCCATG
    GGCTATGAAA TGTCTAAGCC TGACCTCCGG GCTGAACTGG AAGCTGATCT GAAGCTGATC
    TGTGATGGCA AAAAGGACAA ATTTGTGGTT CTAAGGCAGC AAGTGCAGAA ATACAAGCAG
    GTTTTCATTG AAGCGGTGGC TAAAGCAAAG AAATTGGACG AGGCCTTGGC CCAGTACTTT
    GGGAATGGGA CAGAGTTGGC CCAGCAAGAA GATATCTACC CAGCCATGCC AGAGCCCATC
    AGGAAGTGCC CACAGTGCAA CAAGGACATG GTCCTTAAGA CCAAGAAGAA TGGCGGGTTC
    TACCTCAGCT GCATGGGTTT CCCAGAGTGT CGCTCAGCTG TGTGGCTTCC TGACTCGGTG
    CTGGAGGCCA GCAGGGACAG CAGTGTGTGT CCAGTTTGTC AGCCACACCC TGTGTACAGG
    TTAAAGTTAA AGTTTAAGCG CGGTAGCCTT CCCCCGACCA TGCCTCTGGA GTTTGTTTGC
    TGCATCGGCG GATGCGACGA CACCCTGAGG GAGATCCTGG ACCTGAGATT TTCAGGGGGC
    CCCCCCAGGG CTAGCCAGCC CTCTGGCCGC CTGCAGGCTA ACCAGTCCCT GAACAGGATG
    GACAACAGCC AGCACCCCCA GCCTGCTGAC AGCAGACAGA CTGGGTCCTC AAAGGCTCTG
    GCCCAGACCC TCCCACCACC CACGGCTGCT GGTGAAAGCA ATTCTGTGAC CTGCAACTGT
    GGCCAGGAGG CTGTGCTGCT CACTGTCCGT AAGGAGGGCC CCAACCGGGG CCGGCAGTTC
    TTTAAGTGCA ACGGAGGTAG CTGCAACTTC TTCCTGTGGG CAGACAGCCC CAATCCGGGA
    GCAGGAGGGC CTCCTGCCTT GGCATATAGA CCCCTGGGCG CCTCCCTGGG ATGCCCACCA
    GGCCCAGGGA TCCACCTAGG TGGGTTTGGC AACCCTGGTG ATGGCAGTGG TAGTGGCACA
    TCCTGCCTTT GCAGCCAGCC CTCCGTCACA CGGACTGTGC AGAAGGATGG ACCCAACAAG
    GGGCGCCAGT TCCACACATG TGCCAAGCCG AGAGAGCAGC AGTGTGGCTT TTTCCAGTGG
    GTCGATGAGA ACACCGCTCC AGGGACTTCT GGAGCCCCGT CCTGGACAGG AGACAGAGGA
    AGAACCCTGG AGTCGGAAGC CAGAAGCAAA AGGCCCCGGG CCAGTTCCTC AGACATGGGG
    TCCACAGCAA AGAAACCCCG GAAATGCAGC CTTTGCCACC AGCCTGGACA CACCCGTCCC
    TTTTGTCCTC AGAACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu104463
    Clone ID Related Accession (Same CDS sequence) XM_024450903.1
    Accession Version XM_024450903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA topoisomerase 3-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAACTG TGGTAGAGGT CAGATCTAAG CCCAAGAGCA AGTGGCGGCC TCAAGCCTTG 
    GACACTGTGG AGCTTGAGAA GCTGGCTTCT CGAAAGTTGA GAATAAATGC TAAAGAAACC
    ATGAGGATTG CTGAGAAGCT CTACACTCAA GGGTACATCA GCTATCCCCG AACAGAAACA
    AACATTTTTC CCAGAGACTT AAACCTGACG GTGTTGGTGG AACAGCAGAC CCCCGATCCA
    CGCTGGGGGG CCTTTGCCCA GAGCATTCTA GAGCGGGGTG GTCCCACCCC ACGCAATGGG
    AACAAGTCTG ACCAAGCTCA CCCTCCCATT CACCCCACCA AATACACCAA CAACTTACAG
    GGAGATGAAC AGCGACTGTA CGAGTTTATT GTTCGCCATT TCCTGGCTTG CTGCTCCCAG
    GATGCTCAGG GGCAGGAGAC CACAGTGGAG ATCGACATCG CTCAGGAACG CTTTGTGGCC
    CATGGCCTCA TGATTCTGGC CCGAAACTAT CTGGATGTGT ATCCATATGA TCACTGGAGT
    GACAAGATCC TCCCTGTCTA TGAGCAAGGA TCCCACTTTC AGCCCAGCAC CGTGGAGATG
    GTGGACGGGG AGACCAGCCC ACCCAAGCTG CTCACCGAGG CCGACCTCAT TGCCCTCATG
    GAGAAGCATG GCATTGGTAC GGATGCCACT CATGCGGAGC ACATCGAGAC CATCAAAGCC
    CGGATGTACG TGGGCCTCAC CCCAGACAAG CGGTTCCTCC CTGGGCACCT GGGCATGGGA
    CTTGTGGAAG GTTATGATTC CATGGGCTAT GAAATGTCTA AGCCTGACCT CCGGGCTGAA
    CTGGAAGCTG ATCTGAAGCT GATCTGTGAT GGCAAAAAGG ACAAATTTGT GGTTCTAAGG
    CAGCAAGTGC AGAAATACAA GCAGGTTTTC ATTGAAGCGG TGGCTAAAGC AAAGAAATTG
    GACGAGGCCT TGGCCCAGTA CTTTGGGAAT GGGACAGAGT TGGCCCAGCA AGAAGATATC
    TACCCAGCCA TGCCAGAGCC CATCAGGAAG TGCCCACAGT GCAACAAGGA CATGGTCCTT
    AAGACCAAGA AGAATGGCGG GTTCTACCTC AGCTGCATGG GTTTCCCAGA GTGTCGCTCA
    GCTGTGTGGC TTCCTGACTC GGTGCTGGAG GCCAGCAGGG ACAGCAGTGT GTGTCCAGTT
    TGTCAGCCAC ACCCTGTGTA CAGGTTAAAG TTAAAGTTTA AGCGCGGTAG CCTTCCCCCG
    ACCATGCCTC TGGAGTTTGT TTGCTGCATC GGCGGATGCG ACGACACCCT GAGGGAGATC
    CTGGACCTGA GATTTTCAGG GGGCCCCCCC AGGGCTAGCC AGCCCTCTGG CCGCCTGCAG
    GCTAACCAGT CCCTGAACAG GATGGACAAC AGCCAGCACC CCCAGCCTGC TGACAGCAGA
    CAGACTGGGT CCTCAAAGGC TCTGGCCCAG ACCCTCCCAC CACCCACGGC TGCTGGTGAA
    AGCAATTCTG TGACCTGCAA CTGTGGCCAG GAGGCTGTGC TGCTCACTGT CCGTAAGGAG
    GGCCCCAACC GGGGCCGGCA GTTCTTTAAG TGCAACGGAG GTAGCTGCAA CTTCTTCCTG
    TGGGCAGACA GCCCCAATCC GGGAGCAGGA GGGCCTCCTG CCTTGGCATA TAGACCCCTG
    GGCGCCTCCC TGGGATGCCC ACCAGGCCCA GGGATCCACC TAGGTGGGTT TGGCAACCCT
    GGTGATGGCA GTGGTAGTGG CACATCCTGC CTTTGCAGCC AGCCCTCCGT CACACGGACT
    GTGCAGAAGG ATGGACCCAA CAAGGGGCGC CAGTTCCACA CATGTGCCAA GCCGAGAGAG
    CAGCAGTGTG GCTTTTTCCA GTGGGTCGAT GAGAACACCG CTCCAGGGAC TTCTGGAGCC
    CCGTCCTGGA CAGGAGACAG AGGAAGAACC CTGGAGTCGG AAGCCAGAAG CAAAAGGCCC
    CGGGCCAGTT CCTCAGACAT GGGGTCCACA GCAAAGAAAC CCCGGAAATG CAGCCTTTGC
    CACCAGCCTG GACACACCCG TCCCTTTTGT CCTCAGAACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024306671.1
    CDS1167..3251
    Translation

    Target ORF information:

    RefSeq Version XM_024450903.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase III alpha (TOP3A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024450903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCAACTG TGGTAGAGGT CAGATCTAAG CCCAAGAGCA AGTGGCGGCC TCAAGCCTTG 
    GACACTGTGG AGCTTGAGAA GCTGGCTTCT CGAAAGTTGA GAATAAATGC TAAAGAAACC
    ATGAGGATTG CTGAGAAGCT CTACACTCAA GGGTACATCA GCTATCCCCG AACAGAAACA
    AACATTTTTC CCAGAGACTT AAACCTGACG GTGTTGGTGG AACAGCAGAC CCCCGATCCA
    CGCTGGGGGG CCTTTGCCCA GAGCATTCTA GAGCGGGGTG GTCCCACCCC ACGCAATGGG
    AACAAGTCTG ACCAAGCTCA CCCTCCCATT CACCCCACCA AATACACCAA CAACTTACAG
    GGAGATGAAC AGCGACTGTA CGAGTTTATT GTTCGCCATT TCCTGGCTTG CTGCTCCCAG
    GATGCTCAGG GGCAGGAGAC CACAGTGGAG ATCGACATCG CTCAGGAACG CTTTGTGGCC
    CATGGCCTCA TGATTCTGGC CCGAAACTAT CTGGATGTGT ATCCATATGA TCACTGGAGT
    GACAAGATCC TCCCTGTCTA TGAGCAAGGA TCCCACTTTC AGCCCAGCAC CGTGGAGATG
    GTGGACGGGG AGACCAGCCC ACCCAAGCTG CTCACCGAGG CCGACCTCAT TGCCCTCATG
    GAGAAGCATG GCATTGGTAC GGATGCCACT CATGCGGAGC ACATCGAGAC CATCAAAGCC
    CGGATGTACG TGGGCCTCAC CCCAGACAAG CGGTTCCTCC CTGGGCACCT GGGCATGGGA
    CTTGTGGAAG GTTATGATTC CATGGGCTAT GAAATGTCTA AGCCTGACCT CCGGGCTGAA
    CTGGAAGCTG ATCTGAAGCT GATCTGTGAT GGCAAAAAGG ACAAATTTGT GGTTCTAAGG
    CAGCAAGTGC AGAAATACAA GCAGGTTTTC ATTGAAGCGG TGGCTAAAGC AAAGAAATTG
    GACGAGGCCT TGGCCCAGTA CTTTGGGAAT GGGACAGAGT TGGCCCAGCA AGAAGATATC
    TACCCAGCCA TGCCAGAGCC CATCAGGAAG TGCCCACAGT GCAACAAGGA CATGGTCCTT
    AAGACCAAGA AGAATGGCGG GTTCTACCTC AGCTGCATGG GTTTCCCAGA GTGTCGCTCA
    GCTGTGTGGC TTCCTGACTC GGTGCTGGAG GCCAGCAGGG ACAGCAGTGT GTGTCCAGTT
    TGTCAGCCAC ACCCTGTGTA CAGGTTAAAG TTAAAGTTTA AGCGCGGTAG CCTTCCCCCG
    ACCATGCCTC TGGAGTTTGT TTGCTGCATC GGCGGATGCG ACGACACCCT GAGGGAGATC
    CTGGACCTGA GATTTTCAGG GGGCCCCCCC AGGGCTAGCC AGCCCTCTGG CCGCCTGCAG
    GCTAACCAGT CCCTGAACAG GATGGACAAC AGCCAGCACC CCCAGCCTGC TGACAGCAGA
    CAGACTGGGT CCTCAAAGGC TCTGGCCCAG ACCCTCCCAC CACCCACGGC TGCTGGTGAA
    AGCAATTCTG TGACCTGCAA CTGTGGCCAG GAGGCTGTGC TGCTCACTGT CCGTAAGGAG
    GGCCCCAACC GGGGCCGGCA GTTCTTTAAG TGCAACGGAG GTAGCTGCAA CTTCTTCCTG
    TGGGCAGACA GCCCCAATCC GGGAGCAGGA GGGCCTCCTG CCTTGGCATA TAGACCCCTG
    GGCGCCTCCC TGGGATGCCC ACCAGGCCCA GGGATCCACC TAGGTGGGTT TGGCAACCCT
    GGTGATGGCA GTGGTAGTGG CACATCCTGC CTTTGCAGCC AGCCCTCCGT CACACGGACT
    GTGCAGAAGG ATGGACCCAA CAAGGGGCGC CAGTTCCACA CATGTGCCAA GCCGAGAGAG
    CAGCAGTGTG GCTTTTTCCA GTGGGTCGAT GAGAACACCG CTCCAGGGAC TTCTGGAGCC
    CCGTCCTGGA CAGGAGACAG AGGAAGAACC CTGGAGTCGG AAGCCAGAAG CAAAAGGCCC
    CGGGCCAGTT CCTCAGACAT GGGGTCCACA GCAAAGAAAC CCCGGAAATG CAGCCTTTGC
    CACCAGCCTG GACACACCCG TCCCTTTTGT CCTCAGAACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.