TOP1 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP1
Entrez Gene ID	7150
Full Name	DNA topoisomerase I
Synonyms	TOPI
General protein information	Preferred Names DNA topoisomerase I Names DNA topoisomerase 1 topoisomerase (DNA) I type I DNA topoisomerase
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 20 Map Location: 20q12
Summary	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008].
Disorder MIM:	126420
Disorder Html:	DNA topoisomerase I, camptothecin-resistant (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003286.4	NP_003277.1	DNA topoisomerase 1
NM_003286.3	NP_003277.1	DNA topoisomerase 1
NM_003286.2	NP_003277.1	DNA topoisomerase 1

Pathways from BioSystems

Homologies

	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Neurospora crassa	NCU09118	XP_958033.2
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1

Gene Ontology

The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27246	NM_003286.4 Latest version!	Homo sapiens DNA topoisomerase I (TOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
View Old Accession Versions >>
OHu27246	NM_003286.3	Homo sapiens DNA topoisomerase I (TOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
OHu27246	NM_003286.2	Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$265.30 $379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP1 ( NM_003286.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003277.1 Homo sapiens DNA topoisomerase I (TOP1), mRNA.

Price & Availability↑

CloneID	OHu27246
Clone ID Related Accession (Same CDS sequence)	NM_003286.4 , NM_003286.3 , NM_003286.2
Accession Version	NM_003286.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	DNA topoisomerase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA690829.1, AK292943.1 and BC000943.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003286.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U07806.1, SRR1660803.104236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361337.3/ ENSP00000354522.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003277.1
CDS	247..2544
Translation	MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

Target ORF information:

RefSeq Version	NM_003286.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase I (TOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003286.4

ORF Insert Sequence:

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP1 ( NM_003286.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003277.1 Homo sapiens DNA topoisomerase I (TOP1), mRNA.

Price & Availability↑

CloneID	OHu27246
Clone ID Related Accession (Same CDS sequence)	NM_003286.4 , NM_003286.3 , NM_003286.2
Accession Version	NM_003286.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	DNA topoisomerase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA690829.1, AK292943.1 and BC000943.2. This sequence is a reference standard in the RefSeqGene project. On Sep 26, 2016 this sequence version replaced NM_003286.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U07806.1, SRR1803611.24870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003277.1
CDS	251..2548
Translation	MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

Target ORF information:

RefSeq Version	NM_003286.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA topoisomerase I (TOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003286.3

ORF Insert Sequence:

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP1 ( NM_003286.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003277.1 Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.

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CloneID	OHu27246
Clone ID Related Accession (Same CDS sequence)	NM_003286.4 , NM_003286.3 , NM_003286.2
Accession Version	NM_003286.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	DNA topoisomerase 1
Comment

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_003277.1

Translation

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

Target ORF information:

RefSeq Version	NM_003286.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003286.2

ORF Insert Sequence:

ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG 
GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA 
CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA 
CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA 
AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT 
GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA 
GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG 
AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT 
AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA 
CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA 
GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT 
TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT 
GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA 
GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT 
GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC 
AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC 
TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT 
TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG 
GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG 
TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC 
CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC 
TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG 
TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC 
TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC 
ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT 
CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG 
GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC 
GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC 
ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG 
CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC 
CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT 
GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT 
GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG 
AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG 
CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA 
CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT 
GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT 
GAAGACTATG AGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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050-3174-7589(受注専用)

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TOP1 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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